Rw7G025070

TLR4 regulator and MIR-interacting MSAP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011086
Physical Location & Seq
Forward (+)
31330953 .. 31333430
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025070.1

Sequence Viewer

Length: 558 bp
ATGGAAGGGTCAATGCTCGCGACCGTGATTCTGATATTCACCATCTTGACCGTCGCTTCTTCCATCGACGACAAATGCGCCGCCTGCAATGCCGTCGCGGAGGAGCTAGAGTGGGGACTTTCCAAAGAAAAGCCCAGAAACCACTTGGATATGAGACACCGGTTGGACTCCCAAGGTCAACGGCGAGGAAAGGTAATCGACTACAGAGTCAGTGAGCTCAGAGTTGTTGAACTCCTCGATGGACTTTGTGATAAGATGCAGGATTACACTCTTTGCAAGATAGATTCAAACAGACAAGAGTGGATCAAAGTGGATAATTGGGATAACCTCACAACAGATAAACAAGAAGCTAGAGCATATTCAAAGGATATATCAACTTACTGTGGAAGGTTACTTGAGGAAACAGAAGATGAGATTGCAGAATTGATAAAGAAAGGATCTCTTGGAGTAGGAGATGTAAGCAAGGTTCTATGTCAAGATTTGAGCAAACACTGCAGCAAAACAAGTGCTTCACATGAGGAAAATGTTTCTAATGGTGGATCAGATGGAGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3456

Protein Analysis

185

Amino Acids

20.71

Weight (kDa)

4.92

Isoelectric Point (pI)

29.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3456 PF11938 25 - 165 3.6e-36 TLR4 regulator and MIR-interacting MSAP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42480 AT1G42480 AT1G42480
fragaria_vesca FvH4_5g25650 FvH4_5g25650
malus_domestica MD06G1006600.v1.1
prunus_persica Prupe.5G008600_v2.0.a1 Prupe.5G008700_v2.0.a1
pyrus_communis pycom06g00580
rosa_chinensis RchiOBHm_Chr7g0215941
rosa_laevigata RLG00000002605
rosa_multiflora Rmu_co8213036.1_g000001 Rmu_sc0030911.1_g000001
rosa_roxburghii Rroxscaffold_3G00243760
rosa_rugosa Rorug07G0157200
rosa_samantha Rh7AG295200 Rh7BG287200 Rh7CG315300 Rh7DG297900
rosa_wichuraiana Rw7G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AccII CGCG 2 cut(s) 20, 98
AciI CCGC 2 cut(s) 81, 98
AclWI GGATC 3 cut(s) 311, 445, 547
AgeI ACCGGT 1 cut(s) 159
AgsI TTSAA 3 cut(s) 230, 288, 363
AluBI AGCT 3 cut(s) 106, 217, 350
AluI AGCT 3 cut(s) 106, 217, 350
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 3 cut(s) 311, 445, 547
ApeKI GCWGC 1 cut(s) 495
AsiGI ACCGGT 1 cut(s) 159
AspLEI GCGC 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 31
BanII GRGCYC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 507
BccI CCATC 4 cut(s) 50, 71, 233, 539
BceAI ACGGC 2 cut(s) 77, 197
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 2 cut(s) 107, 351
BfmI CTRYAG 2 cut(s) 202, 493
BisI GCNGC 2 cut(s) 81, 496
BlsI GCNGC 2 cut(s) 82, 497
BmsI GCATC 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 172
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bse118I RCCGGY 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 172
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 1 cut(s) 232
BseRI GAGGAG 2 cut(s) 116, 224
BseXI GCAGC 1 cut(s) 507
Bsh1236I CGCG 2 cut(s) 20, 98
Bsh1285I CGRYCG 1 cut(s) 24
BshTI ACCGGT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 160
BslFI GGGAC 1 cut(s) 129
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 3 cut(s) 303, 437, 539
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 2 cut(s) 81, 98
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 2 cut(s) 20, 98
BspMAI CTGCAG 1 cut(s) 497
BspPI GGATC 3 cut(s) 311, 445, 547
BsrDI GCAATG 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 3 cut(s) 303, 437, 539
BssT1I CCWWGG 1 cut(s) 172
Bst4CI ACNGT 3 cut(s) 25, 52, 383
BstAPI GCANNNNNTGC 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 18, 85
BstDEI CTNAG 1 cut(s) 218
BstFNI CGCG 2 cut(s) 20, 98
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 3 cut(s) 306, 440, 542
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 3 cut(s) 303, 437, 539
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 3 cut(s) 84, 89, 492
BstSFI CTRYAG 2 cut(s) 202, 493
BstUI CGCG 2 cut(s) 20, 98
BstV1I GCAGC 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 437
BstYI RGATCY 1 cut(s) 437
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 2 cut(s) 217, 490
BtuMI TCGCGA 1 cut(s) 20
Cac8I GCNNGC 2 cut(s) 18, 85
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 1 cut(s) 159
CspAI ACCGGT 1 cut(s) 159
CviAII CATG 1 cut(s) 515
CviJI RGCY 4 cut(s) 106, 133, 217, 350
CviKI_1 RGCY 4 cut(s) 106, 133, 217, 350
DdeI CTNAG 1 cut(s) 218
DpnI GATC 3 cut(s) 305, 439, 541
DpnII GATC 3 cut(s) 303, 437, 539
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 1 cut(s) 518
FaiI YATR 5 cut(s) 152, 358, 371, 472, 516
FalI AAGNNNNNCTT 2 cut(s) 426, 458
FaqI GGGAC 1 cut(s) 129
FatI CATG 1 cut(s) 514
Fnu4HI GCNGC 2 cut(s) 81, 496
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 2 cut(s) 81, 496
FspBI CTAG 2 cut(s) 107, 351
GlaI GCGC 1 cut(s) 79
GluI GCNGC 2 cut(s) 81, 496
HapII CCGG 1 cut(s) 160
HhaI GCGC 1 cut(s) 80
Hin1II CATG 1 cut(s) 518
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 179
HindII GTYRAC 1 cut(s) 179
HinfI GANTC 4 cut(s) 28, 167, 207, 284
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 179
Hpy188I TCNGA 4 cut(s) 33, 221, 544, 557
Hpy188III TCNNGA 3 cut(s) 19, 46, 476
Hpy8I GTNNAC 1 cut(s) 179
Hpy99I CGWCG 3 cut(s) 56, 71, 98
HpyAV CCTTC 1 cut(s) 381
HpyCH4III ACNGT 3 cut(s) 25, 52, 383
HpyCH4V TGCA 5 cut(s) 87, 259, 276, 419, 495
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 89, 492
HpyF3I CTNAG 1 cut(s) 218
Hsp92II CATG 1 cut(s) 518
HspAI GCGC 1 cut(s) 78
Kzo9I GATC 3 cut(s) 303, 437, 539
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 4 cut(s) 97, 148, 173, 245
Lsp1109I GCAGC 1 cut(s) 507
LweI GCATC 1 cut(s) 246
MaeI CTAG 2 cut(s) 107, 351
MaeIII GTNAC 1 cut(s) 390
MalI GATC 3 cut(s) 305, 439, 541
MboI GATC 3 cut(s) 303, 437, 539
MboII GAAGA 2 cut(s) 51, 419
MflI RGATCY 1 cut(s) 437
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 2 cut(s) 316, 422
MlyI GAGTC 2 cut(s) 161, 216
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 6 cut(s) 94, 179, 245, 338, 391, 511
MspI CCGG 1 cut(s) 160
MvnI CGCG 2 cut(s) 20, 98
MwoI GCNNNNNNNGC 3 cut(s) 84, 89, 492
NdeII GATC 3 cut(s) 303, 437, 539
NlaIII CATG 1 cut(s) 518
NruI TCGCGA 1 cut(s) 20
PfeI GAWTC 2 cut(s) 28, 284
PinAI ACCGGT 1 cut(s) 159
PkrI GCNGC 2 cut(s) 82, 497
PleI GAGTC 2 cut(s) 161, 215
PpsI GAGTC 2 cut(s) 161, 215
Psp124BI GAGCTC 1 cut(s) 219
PsrI GAACNNNNNNTAC 2 cut(s) 450, 482
PstI CTGCAG 1 cut(s) 497
PsuI RGATCY 1 cut(s) 437
RruI TCGCGA 1 cut(s) 20
SacI GAGCTC 1 cut(s) 219
SatI GCNGC 2 cut(s) 81, 496
Sau3AI GATC 3 cut(s) 303, 437, 539
SchI GAGTC 2 cut(s) 161, 216
SduI GDGCHC 1 cut(s) 219
SetI ASST 8 cut(s) 108, 178, 195, 219, 330, 352, 392, 468
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 2 cut(s) 202, 493
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 2 cut(s) 316, 422
SsiI CCGC 2 cut(s) 81, 98
SspMI CTAG 2 cut(s) 107, 351
SstI GAGCTC 1 cut(s) 219
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 3 cut(s) 25, 52, 383
TaqI TCGA 3 cut(s) 66, 198, 237
TasI AATT 2 cut(s) 316, 422
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 28, 284
TscAI CASTG 2 cut(s) 217, 497
TseI GCWGC 1 cut(s) 495
TspRI CASTG 2 cut(s) 217, 497
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XspI CTAG 2 cut(s) 107, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.