Rh7CG315300

TLR4 regulator and MIR-interacting MSAP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0011086
Physical Location & Seq
Forward (+)
33653275 .. 33655333
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG315300.1

Sequence Viewer

Length: 546 bp
ATGCTCGCGACCGTGATTCTGATATTCACCATCTTCACCGTCGCTTCATCCATCGACGACAAATGCGCCGCCTGCAATGCCGTCGCGGAGGAGCTAGAGTGGGGACTTTCCAAAGAAAAGCCCAGAAACCACTTGGATATGAGACACCGGTTGGACTCCCAAGGTCAACGGCGAGGAAAGGTAATCGACTACAGAGTGAGTGAGCTCAGAGTTGTTGAACTCCTCGATGGACTTTGTGATAAGATGCAGGATTACACTCTTCGCAAGATAGATTCAAACAGACAAGAGTGGATCAAAGTGGATAATTGGGATAACCTCACAACAGATAAACAAGAAGCTAGAGCATATTCAAAGGATATATCAACTTACTGTGGAAGGTTACTTGAGGAAACAGAAGATGAGATTGCAGAATTGATAAAGAAAGGATCTCTTGGAGTAGGAGATGTAAGCAAGGTTCTATGTCAAGATTTGAGCAAACACTGCAGCAAAACAAGTGCTTCACATGAGGAAAATGGTTCTAATGGTGGATCAGATGGAGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3456

Protein Analysis

181

Amino Acids

20.35

Weight (kDa)

5.09

Isoelectric Point (pI)

29.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3456 PF11938 21 - 161 3.5e-37 TLR4 regulator and MIR-interacting MSAP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42480 AT1G42480 AT1G42480
fragaria_vesca FvH4_5g25650 FvH4_5g25650
malus_domestica MD06G1006600.v1.1
prunus_persica Prupe.5G008600_v2.0.a1 Prupe.5G008700_v2.0.a1
pyrus_communis pycom06g00580
rosa_chinensis RchiOBHm_Chr7g0215941
rosa_laevigata RLG00000002605
rosa_multiflora Rmu_co8213036.1_g000001 Rmu_sc0030911.1_g000001
rosa_roxburghii Rroxscaffold_3G00243760
rosa_rugosa Rorug07G0157200
rosa_samantha Rh7AG295200 Rh7BG287200 Rh7CG315300 Rh7DG297900
rosa_wichuraiana Rw7G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 8, 86
AciI CCGC 2 cut(s) 69, 86
AclWI GGATC 3 cut(s) 299, 433, 535
AgeI ACCGGT 1 cut(s) 147
AgsI TTSAA 3 cut(s) 218, 276, 351
AluBI AGCT 3 cut(s) 94, 205, 338
AluI AGCT 3 cut(s) 94, 205, 338
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 136
AlwI GGATC 3 cut(s) 299, 433, 535
ApeKI GCWGC 1 cut(s) 483
AsiGI ACCGGT 1 cut(s) 147
AspLEI GCGC 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 19, 28
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 495
BccI CCATC 4 cut(s) 38, 59, 221, 527
BceAI ACGGC 2 cut(s) 65, 185
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 2 cut(s) 95, 339
BfmI CTRYAG 2 cut(s) 190, 481
BisI GCNGC 2 cut(s) 69, 484
BlsI GCNGC 2 cut(s) 70, 485
BmsI GCATC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 147
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bse118I RCCGGY 1 cut(s) 147
Bse3DI GCAATG 1 cut(s) 82
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 1 cut(s) 220
BseRI GAGGAG 2 cut(s) 104, 212
BseXI GCAGC 1 cut(s) 495
Bsh1236I CGCG 2 cut(s) 8, 86
Bsh1285I CGRYCG 1 cut(s) 12
BshTI ACCGGT 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 117
BsmAI GTCTC 1 cut(s) 136
BsmFI GGGAC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 3 cut(s) 291, 425, 527
Bsp68I TCGCGA 1 cut(s) 8
BspACI CCGC 2 cut(s) 69, 86
BspCNI CTCAG 1 cut(s) 219
BspFNI CGCG 2 cut(s) 8, 86
BspMAI CTGCAG 1 cut(s) 485
BspPI GGATC 3 cut(s) 299, 433, 535
BsrDI GCAATG 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 147
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 3 cut(s) 291, 425, 527
BssT1I CCWWGG 1 cut(s) 160
Bst4CI ACNGT 3 cut(s) 13, 40, 371
Bst6I CTCTTC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 6, 73
BstDEI CTNAG 1 cut(s) 206
BstF5I GGATG 1 cut(s) 47
BstFNI CGCG 2 cut(s) 8, 86
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 3 cut(s) 294, 428, 530
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 3 cut(s) 291, 425, 527
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 72, 77, 480
BstSFI CTRYAG 2 cut(s) 190, 481
BstUI CGCG 2 cut(s) 8, 86
BstV1I GCAGC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
BtsCI GGATG 1 cut(s) 47
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 1 cut(s) 478
BtuMI TCGCGA 1 cut(s) 8
Cac8I GCNNGC 2 cut(s) 6, 73
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 1 cut(s) 147
CspAI ACCGGT 1 cut(s) 147
CviAII CATG 1 cut(s) 503
CviJI RGCY 4 cut(s) 94, 121, 205, 338
CviKI_1 RGCY 4 cut(s) 94, 121, 205, 338
DdeI CTNAG 1 cut(s) 206
DpnI GATC 3 cut(s) 293, 427, 529
DpnII GATC 3 cut(s) 291, 425, 527
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 1 cut(s) 506
FaiI YATR 5 cut(s) 140, 346, 359, 460, 504
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FaqI GGGAC 1 cut(s) 117
FatI CATG 1 cut(s) 502
Fnu4HI GCNGC 2 cut(s) 69, 484
FokI GGATG 1 cut(s) 34
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 2 cut(s) 69, 484
FspBI CTAG 2 cut(s) 95, 339
GlaI GCGC 1 cut(s) 67
GluI GCNGC 2 cut(s) 69, 484
HapII CCGG 1 cut(s) 148
HhaI GCGC 1 cut(s) 68
Hin1II CATG 1 cut(s) 506
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 167
HindII GTYRAC 1 cut(s) 167
HinfI GANTC 3 cut(s) 16, 155, 272
HpaII CCGG 1 cut(s) 148
HphI GGTGA 2 cut(s) 19, 28
Hpy166II GTNNAC 1 cut(s) 167
Hpy188I TCNGA 4 cut(s) 21, 209, 532, 545
Hpy188III TCNNGA 2 cut(s) 7, 464
Hpy8I GTNNAC 1 cut(s) 167
Hpy99I CGWCG 3 cut(s) 44, 59, 86
HpyAV CCTTC 1 cut(s) 369
HpyCH4III ACNGT 3 cut(s) 13, 40, 371
HpyCH4V TGCA 4 cut(s) 75, 247, 407, 483
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 77, 480
HpyF3I CTNAG 1 cut(s) 206
Hsp92II CATG 1 cut(s) 506
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 3 cut(s) 291, 425, 527
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 4 cut(s) 85, 136, 161, 233
Lsp1109I GCAGC 1 cut(s) 495
LweI GCATC 1 cut(s) 234
MaeI CTAG 2 cut(s) 95, 339
MaeIII GTNAC 1 cut(s) 378
MalI GATC 3 cut(s) 293, 427, 529
MboI GATC 3 cut(s) 291, 425, 527
MboII GAAGA 3 cut(s) 25, 251, 407
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 2 cut(s) 304, 410
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 6 cut(s) 82, 167, 233, 326, 379, 499
MspI CCGG 1 cut(s) 148
MvnI CGCG 2 cut(s) 8, 86
MwoI GCNNNNNNNGC 3 cut(s) 72, 77, 480
NdeII GATC 3 cut(s) 291, 425, 527
NlaIII CATG 1 cut(s) 506
NruI TCGCGA 1 cut(s) 8
PfeI GAWTC 2 cut(s) 16, 272
PinAI ACCGGT 1 cut(s) 147
PkrI GCNGC 2 cut(s) 70, 485
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 207
PsrI GAACNNNNNNTAC 2 cut(s) 438, 470
PstI CTGCAG 1 cut(s) 485
PsuI RGATCY 1 cut(s) 425
RruI TCGCGA 1 cut(s) 8
SacI GAGCTC 1 cut(s) 207
SatI GCNGC 2 cut(s) 69, 484
Sau3AI GATC 3 cut(s) 291, 425, 527
SchI GAGTC 1 cut(s) 149
SduI GDGCHC 1 cut(s) 207
SetI ASST 8 cut(s) 96, 166, 183, 207, 318, 340, 380, 456
SfaNI GCATC 1 cut(s) 234
SfcI CTRYAG 2 cut(s) 190, 481
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 2 cut(s) 304, 410
SsiI CCGC 2 cut(s) 69, 86
SspMI CTAG 2 cut(s) 95, 339
SstI GAGCTC 1 cut(s) 207
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 3 cut(s) 13, 40, 371
TaqI TCGA 3 cut(s) 54, 186, 225
TasI AATT 2 cut(s) 304, 410
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 16, 272
TscAI CASTG 1 cut(s) 485
TseI GCWGC 1 cut(s) 483
TspDTI ATGAA 1 cut(s) 36
TspRI CASTG 1 cut(s) 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XspI CTAG 2 cut(s) 95, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.