FvH4_5g28070

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015440
Physical Location & Seq
Forward (+)
19293929 .. 19295240
1312 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28070.t1

Sequence Viewer

Length: 1029 bp
ATGTCTAATGAACCCTCCACCACCACCTCCTCCATTGCTGGAGACACCACCACGGCAACCACAGACGACACCATCCCTGAAGACACCACCAACACCAAACCCTCAACCTCATTAGACTCATCCTCGTCCTCCAAATCCCAATCAGCAGCCATCCAATCCCTCTCCGCCATCCTCCCCTCCTTGTCATCATCCTCCACCGCCCGCTCCCTCCTCCACGACCCCCAAACATCCTCCAAAATCTCCTCCCTCCTCAAACAACCCGACTCCGGCGCCGGCGACAACAACCTCTGCCGCTGGCTCTACGACACCTTCCAGTCCTCCGATCCCGACCTCCAGCTCCTCGTCCTCCGCTTCCTCCCCCTCGTCGCCGGCCTCTACCTCTCCCGTGTCCCCCTCCGCATCCCCCTCGCCGGCTTCGAGGCAGTCCTCTTGGCCCTCTATGCTCACGAGACTGCCTCCCGGGGCGGCCAGTCCGTCACCGTCAACGTCCCTGACTTGTCCCACACCAGCCTCTACCACCACGAAACCAAGACTGCCAACGCCGTAAAGAGCCACGCCACCGGGCTCAACCTGGCCGTGATATCACCCAGCTTGGAACCTCACGGCACCATCAGATCCACACGCAGGGCTAGGATCGTCGGCGTCGCACTGGAACTGTACTACACCAAAGTATATGAGATACCGGTCGAGTCCAAGATAGACTTCTGCGAGTTTTGTAAGGTTTGGGCGGGGGGTTACAGTAGTACTGAGGAGGAGGGGATGATGAGGAGGGTTTCGTTGCCATGCGAGCTGATGCAACCAGTTCTGAGGATTTTAGGGCACTGCCTCTTGTGCCCTAATCAGAACAAGAAGCTCACAGAGAAGGCCAGTGAAGCGTGTACGAGTTTATATGCCAGGTCCATGCATGACGTCAATCCCAAGGCAATTCTGGCTATTGGGAGTTTGGTTAGGCTAGCAGACTCCACGCCCAAGCAGGATGACCTTTTCGATCCAACTGACCTTCACAAATCTAGAGTCATCTCTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

343

Amino Acids

37.01

Weight (kDa)

6.2

Isoelectric Point (pI)

58.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 63 - 319 1.1e-44 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G21050
fragaria_vesca FvH4_5g28070
malus_domestica MD11G1179900.v1.1
prunus_persica Prupe.6G113000_v2.0.a1 Prupe.6G152400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220241
rosa_laevigata RLG00000002247
rosa_multiflora Rmu_co8248515.1_g000001 Rmu_sc0001136.1_g000021
rosa_roxburghii Rroxscaffold_3G00239690
rosa_rugosa Rorug07G0191400
rosa_samantha Rh7AG333500 Rh7BG323700 Rh7CG351100 Rh7DG329800
rosa_wichuraiana Rw7G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 912
AccB1I GGYRCC 2 cut(s) 269, 605
AccBSI CCGCTC 1 cut(s) 204
AciI CCGC 8 cut(s) 165, 198, 202, 292, 349, 397, 465, 728
AclWI GGATC 4 cut(s) 317, 609, 641, 983
AcoI YGGCCR 2 cut(s) 466, 573
AcuI CTGAAG 1 cut(s) 99
AcyI GRCGYC 3 cut(s) 270, 642, 909
AfaI GTAC 3 cut(s) 659, 745, 880
AfiI CCNNNNNNNGG 3 cut(s) 266, 410, 624
AgeI ACCGGT 1 cut(s) 682
AjnI CCWGG 2 cut(s) 570, 893
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AluBI AGCT 4 cut(s) 337, 591, 790, 853
AluI AGCT 4 cut(s) 337, 591, 790, 853
Alw26I GTCTC 2 cut(s) 36, 443
AlwI GGATC 4 cut(s) 317, 609, 641, 983
Ama87I CYCGRG 1 cut(s) 459
AoxI GGCC 5 cut(s) 370, 432, 466, 573, 864
ApeKI GCWGC 1 cut(s) 146
ArsI GACNNNNNNTTYG 1 cut(s) 999
AsiGI ACCGGT 1 cut(s) 682
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 2 cut(s) 433, 897
AsuC2I CCSGG 3 cut(s) 460, 461, 562
AsuHPI GGTGA 2 cut(s) 469, 576
AsuNHI GCTAGC 1 cut(s) 952
AvaI CYCGRG 1 cut(s) 459
AvaII GGWCC 1 cut(s) 897
BaeGI GKGCMC 2 cut(s) 822, 836
BanI GGYRCC 2 cut(s) 269, 605
BanII GRGCYC 1 cut(s) 567
BauI CACGAG 1 cut(s) 446
BbsI GAAGAC 1 cut(s) 87
BbvI GCAGC 1 cut(s) 158
BccI CCATC 4 cut(s) 80, 158, 176, 617
BceAI ACGGC 4 cut(s) 69, 527, 560, 619
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BciT130I CCWGG 2 cut(s) 572, 895
BcnI CCSGG 3 cut(s) 460, 461, 562
BcoDI GTCTC 2 cut(s) 36, 443
BfaI CTAG 4 cut(s) 630, 953, 1011, 1027
BfoI RGCGCY 1 cut(s) 273
BisI GCNGC 3 cut(s) 147, 292, 466
BlsI GCNGC 3 cut(s) 148, 293, 467
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 5 cut(s) 460, 461, 562, 572, 895
Bme18I GGWCC 1 cut(s) 897
BmeT110I CYCGRG 1 cut(s) 459
BmgT120I GGNCC 2 cut(s) 433, 897
BmiI GGNNCC 3 cut(s) 271, 597, 607
BmrFI CCNGG 5 cut(s) 460, 461, 562, 572, 895
BmsI GCATC 2 cut(s) 408, 783
BmtI GCTAGC 1 cut(s) 956
BpiI GAAGAC 1 cut(s) 87
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 2 cut(s) 60, 317
BpuMI CCSGG 3 cut(s) 460, 461, 562
BsaHI GRCGYC 3 cut(s) 270, 642, 909
BsaJI CCNNGG 4 cut(s) 51, 459, 460, 918
BsaWI WCCGGW 1 cut(s) 682
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 410, 624
Bse118I RCCGGY 4 cut(s) 272, 368, 410, 682
Bse1I ACTGG 5 cut(s) 313, 469, 654, 800, 867
Bse3DI GCAATG 1 cut(s) 33
BseBI CCWGG 2 cut(s) 572, 895
BseDI CCNNGG 4 cut(s) 51, 459, 460, 918
BseGI GGATG 9 cut(s) 72, 119, 150, 168, 188, 227, 399, 765, 982
BseLI CCNNNNNNNGG 3 cut(s) 266, 410, 624
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 2 cut(s) 738, 797
BseNI ACTGG 5 cut(s) 313, 469, 654, 800, 867
BseRI GAGGAG 8 cut(s) 19, 200, 232, 239, 329, 764, 767, 781
BseSI GKGCMC 2 cut(s) 822, 836
BseXI GCAGC 1 cut(s) 158
BseYI CCCAGC 1 cut(s) 587
Bsh1285I CGRYCG 1 cut(s) 687
BshFI GGCC 5 cut(s) 372, 434, 468, 575, 866
BshNI GGYRCC 2 cut(s) 269, 605
BshTI ACCGGT 1 cut(s) 682
BsiEI CGRYCG 1 cut(s) 687
BsiHKCI CYCGRG 1 cut(s) 459
BsiSI CCGG 7 cut(s) 267, 273, 369, 411, 460, 561, 683
BslFI GGGAC 3 cut(s) 374, 473, 484
BslI CCNNNNNNNGG 3 cut(s) 266, 410, 624
BsmAI GTCTC 2 cut(s) 36, 443
BsmFI GGGAC 3 cut(s) 374, 473, 484
BsnI GGCC 5 cut(s) 372, 434, 468, 575, 866
BsoBI CYCGRG 1 cut(s) 459
Bsp1286I GDGCHC 3 cut(s) 567, 822, 836
Bsp143I GATC 4 cut(s) 322, 614, 633, 988
BspACI CCGC 8 cut(s) 165, 198, 202, 292, 349, 397, 465, 728
BspANI GGCC 5 cut(s) 372, 434, 468, 575, 866
BspCNI CTCAG 2 cut(s) 739, 798
BspLI GGNNCC 3 cut(s) 271, 597, 607
BspOI GCTAGC 1 cut(s) 956
BspPI GGATC 4 cut(s) 317, 609, 641, 983
BspT107I GGYRCC 2 cut(s) 269, 605
BsrBI CCGCTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 33
BsrFI RCCGGY 4 cut(s) 272, 368, 410, 682
BsrI ACTGG 5 cut(s) 313, 469, 654, 800, 867
BssAI RCCGGY 4 cut(s) 272, 368, 410, 682
BssECI CCNNGG 4 cut(s) 51, 459, 460, 918
BssMI GATC 4 cut(s) 322, 614, 633, 988
BssNI GRCGYC 3 cut(s) 270, 642, 909
BssSI CACGAG 1 cut(s) 446
BssT1I CCWWGG 1 cut(s) 918
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 2 cut(s) 572, 895
Bst4CI ACNGT 3 cut(s) 481, 657, 740
BstACI GRCGYC 3 cut(s) 270, 642, 909
BstC8I GCNNGC 7 cut(s) 202, 274, 296, 370, 412, 788, 954
BstDEI CTNAG 2 cut(s) 747, 806
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 9 cut(s) 72, 119, 150, 168, 188, 227, 399, 765, 982
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 4 cut(s) 325, 617, 636, 991
BstMAI GTCTC 2 cut(s) 36, 443
BstMBI GATC 4 cut(s) 322, 614, 633, 988
BstMCI CGRYCG 1 cut(s) 687
BstMWI GCNNNNNNNGC 5 cut(s) 440, 787, 831, 872, 929
BstNI CCWGG 2 cut(s) 572, 895
BstSCI CCNGG 5 cut(s) 458, 459, 560, 570, 893
BstSLI GKGCMC 2 cut(s) 822, 836
BstV1I GCAGC 1 cut(s) 158
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 614
BstYI RGATCY 1 cut(s) 614
BsuRI GGCC 5 cut(s) 372, 434, 468, 575, 866
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 9 cut(s) 72, 119, 150, 168, 188, 227, 399, 765, 982
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 3 cut(s) 647, 820, 874
Cac8I GCNNGC 7 cut(s) 202, 274, 296, 370, 412, 788, 954
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 4 cut(s) 272, 368, 410, 682
Cfr13I GGNCC 2 cut(s) 433, 897
Cfr9I CCCGGG 1 cut(s) 459
CseI GACGC 1 cut(s) 631
Csp6I GTAC 3 cut(s) 658, 744, 879
CspAI ACCGGT 1 cut(s) 682
CviAII CATG 3 cut(s) 783, 901, 905
CviQI GTAC 3 cut(s) 658, 744, 879
DdeI CTNAG 2 cut(s) 747, 806
DinI GGCGCC 1 cut(s) 271
DpnI GATC 4 cut(s) 324, 616, 635, 990
DpnII GATC 4 cut(s) 322, 614, 633, 988
EaeI YGGCCR 2 cut(s) 466, 573
EciI GGCGGA 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 918
Eco24I GRGCYC 1 cut(s) 567
Eco32I GATATC 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 897
Eco57I CTGAAG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 459
EcoRII CCWGG 2 cut(s) 570, 893
EcoRV GATATC 1 cut(s) 582
EcoT14I CCWWGG 1 cut(s) 918
EcoT22I ATGCAT 1 cut(s) 906
EcoT38I GRGCYC 1 cut(s) 567
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 918
FaeI CATG 3 cut(s) 786, 904, 908
FaiI YATR 8 cut(s) 441, 673, 675, 784, 889, 891, 902, 906
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FaqI GGGAC 3 cut(s) 374, 473, 484
FatI CATG 3 cut(s) 782, 900, 904
FauI CCCGC 2 cut(s) 209, 721
Fnu4HI GCNGC 3 cut(s) 147, 292, 466
FokI GGATG 9 cut(s) 59, 106, 137, 155, 175, 214, 386, 772, 989
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 3 cut(s) 147, 292, 466
FspBI CTAG 4 cut(s) 630, 953, 1011, 1027
GlaI GCGC 1 cut(s) 271
GluI GCNGC 3 cut(s) 147, 292, 466
GsaI CCCAGC 1 cut(s) 591
GsuI CTGGAG 2 cut(s) 60, 317
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 5 cut(s) 372, 434, 468, 575, 866
HapII CCGG 7 cut(s) 267, 273, 369, 411, 460, 561, 683
HgaI GACGC 1 cut(s) 631
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 3 cut(s) 270, 642, 909
Hin1II CATG 3 cut(s) 786, 904, 908
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 5 cut(s) 116, 263, 689, 959, 1014
HpaII CCGG 7 cut(s) 267, 273, 369, 411, 460, 561, 683
HphI GGTGA 2 cut(s) 469, 576
Hpy166II GTNNAC 2 cut(s) 484, 879
Hpy188I TCNGA 4 cut(s) 322, 614, 807, 843
Hpy188III TCNNGA 3 cut(s) 326, 446, 1011
Hpy8I GTNNAC 2 cut(s) 484, 879
Hpy99I CGWCG 3 cut(s) 368, 641, 647
HpyAV CCTTC 3 cut(s) 319, 856, 1010
HpyCH4III ACNGT 3 cut(s) 481, 657, 740
HpyCH4IV ACGT 2 cut(s) 486, 909
HpyCH4V TGCA 2 cut(s) 796, 904
HpyF10VI GCNNNNNNNGC 5 cut(s) 440, 787, 831, 872, 929
HpyF3I CTNAG 2 cut(s) 747, 806
HpySE526I ACGT 2 cut(s) 486, 909
Hsp92I GRCGYC 3 cut(s) 270, 642, 909
Hsp92II CATG 3 cut(s) 786, 904, 908
HspAI GCGC 1 cut(s) 270
KasI GGCGCC 1 cut(s) 269
KroI GCCGGC 3 cut(s) 272, 368, 410
KroNI GCCGGC 3 cut(s) 274, 370, 412
Kzo9I GATC 4 cut(s) 322, 614, 633, 988
LmnI GCTCC 2 cut(s) 209, 342
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 2 cut(s) 408, 783
MaeI CTAG 4 cut(s) 630, 953, 1011, 1027
MaeII ACGT 2 cut(s) 486, 909
MaeIII GTNAC 2 cut(s) 475, 734
MalI GATC 4 cut(s) 324, 616, 635, 990
MbiI CCGCTC 1 cut(s) 204
MboI GATC 4 cut(s) 322, 614, 633, 988
MboII GAAGA 1 cut(s) 92
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 3 cut(s) 567, 822, 836
MluCI AATT 1 cut(s) 924
Mly113I GGCGCC 1 cut(s) 270
MlyI GAGTC 5 cut(s) 110, 257, 698, 953, 1023
MmeI TCCRAC 1 cut(s) 1016
Mph1103I ATGCAT 1 cut(s) 906
MreI CGCCGGCG 1 cut(s) 272
MroNI GCCGGC 3 cut(s) 272, 368, 410
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 7 cut(s) 267, 273, 369, 411, 460, 561, 683
MspR9I CCNGG 5 cut(s) 460, 461, 562, 572, 895
MvaI CCWGG 2 cut(s) 572, 895
MwoI GCNNNNNNNGC 5 cut(s) 440, 787, 831, 872, 929
NaeI GCCGGC 3 cut(s) 274, 370, 412
NarI GGCGCC 1 cut(s) 270
NciI CCSGG 3 cut(s) 460, 461, 562
NdeII GATC 4 cut(s) 322, 614, 633, 988
NgoMIV GCCGGC 3 cut(s) 272, 368, 410
NheI GCTAGC 1 cut(s) 952
NlaIII CATG 3 cut(s) 786, 904, 908
NlaIV GGNNCC 3 cut(s) 271, 597, 607
NmuCI GTSAC 1 cut(s) 475
NsiI ATGCAT 1 cut(s) 906
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PdiI GCCGGC 3 cut(s) 274, 370, 412
PinAI ACCGGT 1 cut(s) 682
PkrI GCNGC 3 cut(s) 148, 293, 467
PleI GAGTC 5 cut(s) 110, 257, 697, 953, 1022
PluTI GGCGCC 1 cut(s) 273
PpsI GAGTC 5 cut(s) 110, 257, 697, 953, 1022
Psp6I CCWGG 2 cut(s) 570, 893
PspFI CCCAGC 1 cut(s) 587
PspGI CCWGG 2 cut(s) 570, 893
PspN4I GGNNCC 3 cut(s) 271, 597, 607
PspPI GGNCC 2 cut(s) 433, 897
PsuI RGATCY 1 cut(s) 614
RsaI GTAC 3 cut(s) 659, 745, 880
RsaNI GTAC 3 cut(s) 658, 744, 879
SatI GCNGC 3 cut(s) 147, 292, 466
Sau3AI GATC 4 cut(s) 322, 614, 633, 988
Sau96I GGNCC 2 cut(s) 433, 897
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 5 cut(s) 110, 257, 698, 953, 1023
ScrFI CCNGG 5 cut(s) 460, 461, 562, 572, 895
SduI GDGCHC 3 cut(s) 567, 822, 836
SfaNI GCATC 2 cut(s) 408, 783
SfoI GGCGCC 1 cut(s) 271
SgrAI CRCCGGYG 1 cut(s) 272
SinI GGWCC 1 cut(s) 897
SmaI CCCGGG 1 cut(s) 461
Sse9I AATT 1 cut(s) 924
SsiI CCGC 8 cut(s) 165, 198, 202, 292, 349, 397, 465, 728
SspDI GGCGCC 1 cut(s) 269
SspMI CTAG 4 cut(s) 630, 953, 1011, 1027
StyD4I CCNGG 5 cut(s) 458, 459, 560, 570, 893
StyI CCWWGG 1 cut(s) 918
TaaI ACNGT 3 cut(s) 481, 657, 740
TaiI ACGT 2 cut(s) 489, 912
TaqI TCGA 3 cut(s) 417, 687, 987
TasI AATT 1 cut(s) 924
TatI WGTACW 2 cut(s) 657, 743
TauI GCSGC 2 cut(s) 294, 468
TscAI CASTG 3 cut(s) 654, 827, 874
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 1 cut(s) 24
TspGWI ACGGA 1 cut(s) 463
TspMI CCCGGG 1 cut(s) 459
TspRI CASTG 3 cut(s) 654, 827, 874
VpaK11BI GGWCC 1 cut(s) 897
XbaI TCTAGA 1 cut(s) 1010
XcmI CCANNNNNNNNNTGG 1 cut(s) 925
XmaI CCCGGG 1 cut(s) 459
XspI CTAG 4 cut(s) 630, 953, 1011, 1027
ZraI GACGTC 1 cut(s) 910
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.