Rh7AG333500

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0015440
Physical Location & Seq
Forward (+)
38474333 .. 38479980
5648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG333500.1

Sequence Viewer

Length: 1161 bp
ATGTCTAACGATCCCTCCACCACCACCTCATCCATTGCTGGAGACACCACCACGGCAACCACAGACGATACCATCAGCACCGCCACCACCATCCCCGAAGACGCCACCAGTTCGACCGCCCTAACCTCCACCACCAAAGCCTCAACCTCATTAGACTCCTCCTCATCCTCAACCTCAACCTCCTCCAAGCCCCAAGCAGCAGCAATCCAATCCCTCTCCGCCATCCTCCCCTCCCTCTCCTCCACCTCCCACACCACCGCCCGTTCCCTCCTCCACGACCCCCGCACATCCTCCCAAATCTCCTCCCTCCTCAAACAACCCGACTCCGGCGCCGGCGACAACAACCTCTGCCGCTGGCTCTACGACACCTTCCAGTCCTCCGATCCCGACCTCCAGCTCCTCGTCCTCCGCTTCCTCCCCCTCGTCGCCGGCCTCTACCTCTCCCGCGTCCCCCTCCGCATCCCCCTCGCCGGCTTCGAGGCCGTCCTCCTGGCCCTCTACGCTCACGAGACCGCCTCCCGCGACGGTCATTCCGTCACCGTCAACGTTCCCGACGTGTCCCACCCCAGCCTCTACCACGAAACCAACGCCGCCGCCGTCAAGAGCAACGCCACCGGCCTCAACTTGACCGTTATATCACCCAGCTTGGAGCCTCACGGCACCGTCAGATCCACACGGAGGGCTAGGATCGTCGGGGTGGCGCTGGAGCTGTACTACACCAAAATATACGAAATGCCAGTCGAGTCCAAGATCGATTTCTGCGAGTTTTGTAAGGTTTGGGCTGGTCAGGACGGAGAAATGTACAAACAGGACGTGGAAAAGAGTAGTGGAAACAAAATAAACGAGGGAAAGGCGGAGGGTAGTTTGGGGAATAATGATAAGGAAGGGATGGGGAGGGTTCCCTTGCCATGCGAATTGCTGCAACCAGTTTTGAGGATTTTAGGGCACTGCCTTTTGTGCCCTAATCAGAACCAGAAGCTCACTGATAAGGCGAGTGAAGCGTGTAGGAGTCTGTATGCGAGGTCTATGCATGACATCAATCCCAAGGCAATTTTGGCTACTGGGAGTTTGCTTAGGCTAGCTAACATGGCTTTCGACTCCACCAAGCAAGACCTTTTTGATCCAACTGACGTTCCCAAATCTAGAGTCATCTCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

386

Amino Acids

41.4

Weight (kDa)

5.86

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 83 - 365 1.2e-47 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G21050
fragaria_vesca FvH4_5g28070
malus_domestica MD11G1179900.v1.1
prunus_persica Prupe.6G113000_v2.0.a1 Prupe.6G152400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220241
rosa_laevigata RLG00000002247
rosa_multiflora Rmu_co8248515.1_g000001 Rmu_sc0001136.1_g000021
rosa_roxburghii Rroxscaffold_3G00239690
rosa_rugosa Rorug07G0191400
rosa_samantha Rh7AG333500 Rh7BG323700 Rh7CG351100 Rh7DG329800
rosa_wichuraiana Rw7G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 329, 659
AccII CGCG 2 cut(s) 447, 522
AclI AACGTT 1 cut(s) 546
AclWI GGATC 5 cut(s) 5, 377, 663, 695, 1115
AcyI GRCGYC 2 cut(s) 102, 330
AfaI GTAC 2 cut(s) 713, 803
AfiI CCNNNNNNNGG 3 cut(s) 326, 470, 678
AflIII ACRYGT 1 cut(s) 555
AjiI CACGTC 2 cut(s) 556, 814
AjnI CCWGG 1 cut(s) 489
AjuI GAANNNNNNNTTGG 2 cut(s) 740, 772
AluBI AGCT 5 cut(s) 397, 645, 709, 979, 1082
AluI AGCT 5 cut(s) 397, 645, 709, 979, 1082
Alw26I GTCTC 2 cut(s) 36, 503
AlwI GGATC 5 cut(s) 5, 377, 663, 695, 1115
AoxI GGCC 4 cut(s) 430, 480, 492, 616
ApeKI GCWGC 3 cut(s) 197, 200, 919
ArsI GACNNNNNNTTYG 3 cut(s) 723, 755, 1131
AspLEI GCGC 2 cut(s) 332, 703
AspS9I GGNCC 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 529, 630
AsuNHI GCTAGC 1 cut(s) 1078
BaeGI GKGCMC 2 cut(s) 948, 962
BanI GGYRCC 2 cut(s) 329, 659
BauI CACGAG 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 3 cut(s) 209, 212, 906
BccI CCATC 4 cut(s) 80, 98, 230, 883
BceAI ACGGC 4 cut(s) 69, 467, 581, 673
BcgI CGANNNNNNTGC 4 cut(s) 448, 482, 1009, 1043
BciT130I CCWGG 1 cut(s) 491
BcoDI GTCTC 2 cut(s) 36, 503
BfaI CTAG 3 cut(s) 684, 1079, 1143
BfoI RGCGCY 2 cut(s) 333, 704
BisI GCNGC 6 cut(s) 198, 201, 352, 591, 594, 920
BlsI GCNGC 6 cut(s) 199, 202, 353, 592, 595, 921
Bme1390I CCNGG 1 cut(s) 491
BmgBI CACGTC 2 cut(s) 556, 814
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 4 cut(s) 331, 651, 661, 900
BmrFI CCNGG 1 cut(s) 491
BmrI ACTGGG 1 cut(s) 1071
BmsI GCATC 1 cut(s) 468
BmtI GCTAGC 1 cut(s) 1082
BmuI ACTGGG 1 cut(s) 1071
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpmI CTGGAG 3 cut(s) 60, 377, 725
Bpu10I CCTNAGC 1 cut(s) 1073
Bsa29I ATCGAT 1 cut(s) 753
BsaHI GRCGYC 2 cut(s) 102, 330
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 2 cut(s) 51, 1044
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 470, 678
Bse118I RCCGGY 4 cut(s) 332, 428, 470, 614
Bse1I ACTGG 5 cut(s) 108, 373, 737, 926, 1066
Bse3DI GCAATG 1 cut(s) 33
BseBI CCWGG 1 cut(s) 491
BseCI ATCGAT 1 cut(s) 753
BseDI CCNNGG 2 cut(s) 51, 1044
BseGI GGATG 7 cut(s) 29, 90, 164, 222, 287, 459, 894
BseLI CCNNNNNNNGG 3 cut(s) 326, 470, 678
BseMI GCAATG 1 cut(s) 33
BseNI ACTGG 5 cut(s) 108, 373, 737, 926, 1066
BseRI GAGGAG 8 cut(s) 148, 151, 172, 229, 260, 292, 299, 389
BseSI GKGCMC 2 cut(s) 948, 962
BseXI GCAGC 3 cut(s) 209, 212, 906
BseYI CCCAGC 2 cut(s) 566, 641
Bsh1236I CGCG 2 cut(s) 447, 522
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 4 cut(s) 432, 482, 494, 618
BshNI GGYRCC 2 cut(s) 329, 659
BshVI ATCGAT 1 cut(s) 753
BsiEI CGRYCG 1 cut(s) 117
BsiSI CCGG 5 cut(s) 327, 333, 429, 471, 615
BslFI GGGAC 2 cut(s) 434, 544
BslI CCNNNNNNNGG 3 cut(s) 326, 470, 678
BsmAI GTCTC 2 cut(s) 36, 503
BsmFI GGGAC 2 cut(s) 434, 544
BsnI GGCC 4 cut(s) 432, 482, 494, 618
Bso31I GGTCTC 1 cut(s) 503
Bsp1286I GDGCHC 2 cut(s) 948, 962
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 6 cut(s) 10, 382, 668, 687, 750, 1120
BspANI GGCC 4 cut(s) 432, 482, 494, 618
BspDI ATCGAT 1 cut(s) 753
BspFNI CGCG 2 cut(s) 447, 522
BspLI GGNNCC 4 cut(s) 331, 651, 661, 900
BspOI GCTAGC 1 cut(s) 1082
BspPI GGATC 5 cut(s) 5, 377, 663, 695, 1115
BspT107I GGYRCC 2 cut(s) 329, 659
BspTNI GGTCTC 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 33
BsrFI RCCGGY 4 cut(s) 332, 428, 470, 614
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 5 cut(s) 108, 373, 737, 926, 1066
BssAI RCCGGY 4 cut(s) 332, 428, 470, 614
BssECI CCNNGG 2 cut(s) 51, 1044
BssMI GATC 6 cut(s) 10, 382, 668, 687, 750, 1120
BssNI GRCGYC 2 cut(s) 102, 330
BssSI CACGAG 1 cut(s) 506
BssT1I CCWWGG 1 cut(s) 1044
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 491
Bst4CI ACNGT 4 cut(s) 527, 541, 631, 664
BstACI GRCGYC 2 cut(s) 102, 330
BstAUI TGTACA 1 cut(s) 801
BstC8I GCNNGC 5 cut(s) 334, 356, 430, 472, 1080
BstDEI CTNAG 1 cut(s) 1073
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 7 cut(s) 29, 90, 164, 222, 287, 459, 894
BstFNI CGCG 2 cut(s) 447, 522
BstH2I RGCGCY 2 cut(s) 333, 704
BstHHI GCGC 2 cut(s) 332, 703
BstKTI GATC 6 cut(s) 13, 385, 671, 690, 753, 1123
BstMAI GTCTC 2 cut(s) 36, 503
BstMBI GATC 6 cut(s) 10, 382, 668, 687, 750, 1120
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 5 cut(s) 500, 957, 998, 1055, 1088
BstNI CCWGG 1 cut(s) 491
BstSCI CCNGG 1 cut(s) 489
BstSLI GKGCMC 2 cut(s) 948, 962
BstUI CGCG 2 cut(s) 447, 522
BstV1I GCAGC 3 cut(s) 209, 212, 906
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
Bsu15I ATCGAT 1 cut(s) 753
BsuRI GGCC 4 cut(s) 432, 482, 494, 618
BsuTUI ATCGAT 1 cut(s) 753
BtgI CCRYGG 1 cut(s) 51
BtrI CACGTC 2 cut(s) 556, 814
BtsCI GGATG 7 cut(s) 29, 90, 164, 222, 287, 459, 894
BtsI GCAGTG 1 cut(s) 946
BtsIMutI CAGTG 2 cut(s) 946, 981
Cac8I GCNNGC 5 cut(s) 334, 356, 430, 472, 1080
CfoI GCGC 2 cut(s) 332, 703
Cfr10I RCCGGY 4 cut(s) 332, 428, 470, 614
Cfr13I GGNCC 1 cut(s) 493
ClaI ATCGAT 1 cut(s) 753
CseI GACGC 2 cut(s) 110, 436
Csp6I GTAC 2 cut(s) 712, 802
CviAII CATG 3 cut(s) 909, 1031, 1087
CviQI GTAC 2 cut(s) 712, 802
DdeI CTNAG 1 cut(s) 1073
DinI GGCGCC 1 cut(s) 331
DpnI GATC 6 cut(s) 12, 384, 670, 689, 752, 1122
DpnII GATC 6 cut(s) 10, 382, 668, 687, 750, 1120
EciI GGCGGA 2 cut(s) 208, 869
Eco130I CCWWGG 1 cut(s) 1044
Eco31I GGTCTC 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 1044
EcoT22I ATGCAT 1 cut(s) 1032
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 1044
FaeI CATG 3 cut(s) 912, 1034, 1090
FaiI YATR 8 cut(s) 635, 727, 910, 1017, 1028, 1032, 1088, 1157
FaqI GGGAC 2 cut(s) 434, 544
FatI CATG 3 cut(s) 908, 1030, 1086
FauI CCCGC 3 cut(s) 290, 452, 527
Fnu4HI GCNGC 6 cut(s) 198, 201, 352, 591, 594, 920
FokI GGATG 7 cut(s) 16, 77, 151, 209, 274, 446, 901
Fsp4HI GCNGC 6 cut(s) 198, 201, 352, 591, 594, 920
FspBI CTAG 3 cut(s) 684, 1079, 1143
GlaI GCGC 2 cut(s) 331, 702
GluI GCNGC 6 cut(s) 198, 201, 352, 591, 594, 920
GsaI CCCAGC 2 cut(s) 570, 645
GsuI CTGGAG 3 cut(s) 60, 377, 725
HaeII RGCGCY 2 cut(s) 333, 704
HaeIII GGCC 4 cut(s) 432, 482, 494, 618
HapII CCGG 5 cut(s) 327, 333, 429, 471, 615
HgaI GACGC 2 cut(s) 110, 436
HhaI GCGC 2 cut(s) 332, 703
Hin1I GRCGYC 2 cut(s) 102, 330
Hin1II CATG 3 cut(s) 912, 1034, 1090
Hin6I GCGC 2 cut(s) 330, 701
HinP1I GCGC 2 cut(s) 330, 701
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HinfI GANTC 6 cut(s) 155, 323, 743, 1009, 1097, 1146
HpaII CCGG 5 cut(s) 327, 333, 429, 471, 615
HphI GGTGA 2 cut(s) 529, 630
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 3 cut(s) 382, 668, 969
Hpy188III TCNNGA 6 cut(s) 386, 506, 551, 601, 788, 1143
Hpy8I GTNNAC 1 cut(s) 544
Hpy99I CGWCG 4 cut(s) 428, 527, 557, 695
HpyAV CCTTC 2 cut(s) 379, 878
HpyCH4III ACNGT 4 cut(s) 527, 541, 631, 664
HpyCH4IV ACGT 4 cut(s) 546, 555, 813, 1131
HpyCH4V TGCA 2 cut(s) 922, 1030
HpyF10VI GCNNNNNNNGC 5 cut(s) 500, 957, 998, 1055, 1088
HpyF3I CTNAG 1 cut(s) 1073
HpySE526I ACGT 4 cut(s) 546, 555, 813, 1131
Hsp92I GRCGYC 2 cut(s) 102, 330
Hsp92II CATG 3 cut(s) 912, 1034, 1090
HspAI GCGC 2 cut(s) 330, 701
KasI GGCGCC 1 cut(s) 329
KroI GCCGGC 3 cut(s) 332, 428, 470
KroNI GCCGGC 3 cut(s) 334, 430, 472
Kzo9I GATC 6 cut(s) 10, 382, 668, 687, 750, 1120
LmnI GCTCC 3 cut(s) 402, 649, 706
Lsp1109I GCAGC 3 cut(s) 209, 212, 906
LweI GCATC 1 cut(s) 468
MaeI CTAG 3 cut(s) 684, 1079, 1143
MaeII ACGT 4 cut(s) 546, 555, 813, 1131
MaeIII GTNAC 1 cut(s) 535
MalI GATC 6 cut(s) 12, 384, 670, 689, 752, 1122
MboI GATC 6 cut(s) 10, 382, 668, 687, 750, 1120
MboII GAAGA 1 cut(s) 110
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 2 cut(s) 948, 962
MluCI AATT 2 cut(s) 914, 1050
Mly113I GGCGCC 1 cut(s) 330
MlyI GAGTC 6 cut(s) 149, 317, 752, 1018, 1091, 1155
MmeI TCCRAC 1 cut(s) 1148
Mph1103I ATGCAT 1 cut(s) 1032
MreI CGCCGGCG 1 cut(s) 332
MroNI GCCGGC 3 cut(s) 332, 428, 470
MslI CAYNNNNRTG 1 cut(s) 1154
MspA1I CMGCKG 1 cut(s) 354
MspI CCGG 5 cut(s) 327, 333, 429, 471, 615
MspR9I CCNGG 1 cut(s) 491
MvaI CCWGG 1 cut(s) 491
MvnI CGCG 2 cut(s) 447, 522
MwoI GCNNNNNNNGC 5 cut(s) 500, 957, 998, 1055, 1088
NaeI GCCGGC 3 cut(s) 334, 430, 472
NarI GGCGCC 1 cut(s) 330
NdeII GATC 6 cut(s) 10, 382, 668, 687, 750, 1120
NgoMIV GCCGGC 3 cut(s) 332, 428, 470
NheI GCTAGC 1 cut(s) 1078
NlaIII CATG 3 cut(s) 912, 1034, 1090
NlaIV GGNNCC 4 cut(s) 331, 651, 661, 900
NmuCI GTSAC 1 cut(s) 535
NsiI ATGCAT 1 cut(s) 1032
PcsI WCGNNNNNNNCGW 5 cut(s) 474, 531, 552, 594, 759
PdiI GCCGGC 3 cut(s) 334, 430, 472
PkrI GCNGC 6 cut(s) 199, 202, 353, 592, 595, 921
PleI GAGTC 6 cut(s) 149, 317, 751, 1017, 1091, 1154
PluTI GGCGCC 1 cut(s) 333
PpsI GAGTC 6 cut(s) 149, 317, 751, 1017, 1091, 1154
Psp1406I AACGTT 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 489
PspFI CCCAGC 2 cut(s) 566, 641
PspGI CCWGG 1 cut(s) 489
PspN4I GGNNCC 4 cut(s) 331, 651, 661, 900
PspPI GGNCC 1 cut(s) 493
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 2 cut(s) 713, 803
RsaNI GTAC 2 cut(s) 712, 802
RseI CAYNNNNRTG 1 cut(s) 1154
SatI GCNGC 6 cut(s) 198, 201, 352, 591, 594, 920
Sau3AI GATC 6 cut(s) 10, 382, 668, 687, 750, 1120
Sau96I GGNCC 1 cut(s) 493
SchI GAGTC 6 cut(s) 149, 317, 752, 1018, 1091, 1155
ScrFI CCNGG 1 cut(s) 491
SduI GDGCHC 2 cut(s) 948, 962
SfaNI GCATC 1 cut(s) 468
SfoI GGCGCC 1 cut(s) 331
SgrAI CRCCGGYG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 1154
Sse9I AATT 2 cut(s) 914, 1050
SspDI GGCGCC 1 cut(s) 329
SspMI CTAG 3 cut(s) 684, 1079, 1143
StyD4I CCNGG 1 cut(s) 489
StyI CCWWGG 1 cut(s) 1044
TaaI ACNGT 4 cut(s) 527, 541, 631, 664
TaiI ACGT 4 cut(s) 549, 558, 816, 1134
TaqI TCGA 5 cut(s) 113, 477, 741, 753, 1095
TasI AATT 2 cut(s) 914, 1050
TatI WGTACW 2 cut(s) 711, 801
TauI GCSGC 3 cut(s) 354, 593, 596
TscAI CASTG 2 cut(s) 953, 988
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 3 cut(s) 197, 200, 919
Tsp45I GTSAC 1 cut(s) 535
TspGWI ACGGA 3 cut(s) 523, 691, 807
TspRI CASTG 2 cut(s) 953, 988
XbaI TCTAGA 1 cut(s) 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 1051
XspI CTAG 3 cut(s) 684, 1079, 1143
Zsp2I ATGCAT 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.