MD11G1179900.v1.1

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015440
Physical Location & Seq
Reverse (-)
21835328 .. 21836479
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1179900.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGTCGAACGATCCCTCCACCTCATCCACCGCAGAAGATCATCAAGACACCATCAGTTCCACCACCACCACCACCACCACCCCAACAATTACCACTTCTGCCTCATCAGACCACTCATCATCCCGCTCCAAAGCCCAATCAACAATCCAGTCCCTCTCAACCATCCTCCCCTCTCTCCCTCCTTCCCTCGCCTCCTCCTCCAACCCTTCCCGCTCCCTCCTCCATGACCACCACACCGCCTCCAACATCTCCTCCCTTCTCCGCCTACCCGACTCCGGTGCAGGCGACAACAACCTCTGCCGTTGGCTCTACGACACCTTCCAATCCTCCGATAACGACCTCCAACTTGTGGTCCTTCGCTTCCTTCCCATCATTGCTGGTGTCTACCTCTCCCGCGTGTCTCTTCGTATACCCATAGCCGGCTTTGAGGCTGTTCTGTTAGCCCTCTACGCCCACGAAACCACCACACGTGCTAGCCAGGCCATTACCGTCAACGTGCCTGACTTGTCTCACCCCAGCCTCTACCACGAAACAAAGGCACCCATTAAGAACAATTCGACAGGCCTAAACTTGGCAGTTATATCCCCCAGCTTGGAACCCTATGGCACAGTGAGGTCCACAAGGAGGGCCAGGATTGTTGGGGTTGCGCTTGAGTTGTACTACAGCAAGATATGTGAAATGCCGGTGCAGTCTAAAATCGAATTTTGTGAGTTTTGTAGGGTTTGGGCTGGTCAAGACGGCGAAATGTACAAGGAGATGACTGGTAGTAGTAGTGGTAGCAGCAGCACTAATAAACGTAAAGAAGAGGGTGGAAAGTGTGAAGTACAGGAGGATGGAAATGATGAGAATAAGGAAGGTGGTTCGGGAAAGGCTGCGGTGGGCGTGGGCATGGGGAGAATTCCACTGCCGTGGGAATTACTGCAACCGGTTTTGCGAATTTTGGGACACTGCCTTTTGTGTCCAAACCAAAACAAGGAGCTGATTGATAAGGCGAGCAAGGCTTGTACCAGTTTGTATGCGAGGTCTATGCATGATATCAATCCCAAGGCTATTTTGGCCACTAGGAGTCTGCTTAGGCTGACCAACACGGCGGCCAAAGATGATATTTTTGATCCAACTGAAATTCCTAAATCTCGAGTTATCAGTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

384

Amino Acids

41.46

Weight (kDa)

6.56

Isoelectric Point (pI)

61.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 65 - 367 5.2e-49 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G21050
fragaria_vesca FvH4_5g28070
malus_domestica MD11G1179900.v1.1
prunus_persica Prupe.6G113000_v2.0.a1 Prupe.6G152400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220241
rosa_laevigata RLG00000002247
rosa_multiflora Rmu_co8248515.1_g000001 Rmu_sc0001136.1_g000021
rosa_roxburghii Rroxscaffold_3G00239690
rosa_rugosa Rorug07G0191400
rosa_samantha Rh7AG333500 Rh7BG323700 Rh7CG351100 Rh7DG329800
rosa_wichuraiana Rw7G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 538
AccB7I CCANNNNNTGG 1 cut(s) 349
AccBSI CCGCTC 2 cut(s) 126, 213
AccI GTMKAC 2 cut(s) 384, 409
AccII CGCG 1 cut(s) 396
AciI CCGC 8 cut(s) 30, 124, 211, 237, 262, 394, 875, 1091
AclWI GGATC 2 cut(s) 5, 1106
AcoI YGGCCR 2 cut(s) 1056, 1092
AcsI RAATTY 4 cut(s) 701, 897, 936, 1122
AcvI CACGTG 1 cut(s) 470
AfaI GTAC 4 cut(s) 659, 749, 825, 1006
AfiI CCNNNNNNNGG 7 cut(s) 275, 349, 419, 571, 592, 624, 973
AflIII ACRYGT 1 cut(s) 467
AgeI ACCGGT 1 cut(s) 925
AjnI CCWGG 2 cut(s) 477, 629
AleI CACNNNNGTG 1 cut(s) 907
AloI GAACNNNNNNTCC 1 cut(s) 31
AluBI AGCT 2 cut(s) 591, 979
AluI AGCT 2 cut(s) 591, 979
Alw26I GTCTC 2 cut(s) 405, 513
AlwI GGATC 2 cut(s) 5, 1106
Ama87I CYCGRG 1 cut(s) 1134
AoxI GGCC 5 cut(s) 480, 562, 627, 1056, 1092
ApeKI GCWGC 3 cut(s) 780, 783, 872
ApoI RAATTY 4 cut(s) 701, 897, 936, 1122
AsiGI ACCGGT 1 cut(s) 925
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 3 cut(s) 352, 615, 627
AsuHPI GGTGA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 1134
AvaII GGWCC 2 cut(s) 352, 615
BalI TGGCCA 1 cut(s) 1058
BanI GGYRCC 1 cut(s) 538
BbrPI CACGTG 1 cut(s) 470
BbvI GCAGC 3 cut(s) 792, 795, 859
BccI CCATC 4 cut(s) 59, 170, 377, 827
BceAI ACGGC 4 cut(s) 285, 754, 892, 1104
BcgI CGANNNNNNTGC 4 cut(s) 260, 294, 1009, 1043
BciT130I CCWGG 2 cut(s) 479, 631
BcoDI GTCTC 2 cut(s) 405, 513
BfaI CTAG 3 cut(s) 474, 1062, 1150
BfmI CTRYAG 1 cut(s) 661
BisI GCNGC 4 cut(s) 781, 784, 873, 1092
BlsI GCNGC 4 cut(s) 782, 785, 874, 1093
Bme1390I CCNGG 2 cut(s) 479, 631
Bme18I GGWCC 2 cut(s) 352, 615
BmeT110I CYCGRG 1 cut(s) 1134
BmgT120I GGNCC 3 cut(s) 352, 615, 627
BmiI GGNNCC 2 cut(s) 540, 597
BmrFI CCNGG 2 cut(s) 479, 631
BmtI GCTAGC 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 1073
BpuEI CTTGAG 1 cut(s) 671
BsaAI YACGTR 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 1038
BsaJI CCNNGG 2 cut(s) 908, 1044
BsaWI WCCGGW 2 cut(s) 275, 925
BsaXI ACNNNNNCTCC 5 cut(s) 29, 224, 236, 254, 266
Bsc4I CCNNNNNNNGG 7 cut(s) 275, 349, 419, 571, 592, 624, 973
Bse118I RCCGGY 3 cut(s) 419, 682, 925
Bse1I ACTGG 3 cut(s) 148, 766, 1008
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 1038
BseBI CCWGG 2 cut(s) 479, 631
BseDI CCNNGG 2 cut(s) 908, 1044
BseGI GGATG 4 cut(s) 23, 119, 162, 838
BseJI GATNNNNATC 1 cut(s) 1038
BseLI CCNNNNNNNGG 7 cut(s) 275, 349, 419, 571, 592, 624, 973
BseMI GCAATG 1 cut(s) 372
BseNI ACTGG 3 cut(s) 148, 766, 1008
BseRI GAGGAG 4 cut(s) 184, 187, 209, 241
BseXI GCAGC 3 cut(s) 792, 795, 859
BseYI CCCAGC 2 cut(s) 515, 587
BsgI GTGCAG 2 cut(s) 300, 707
Bsh1236I CGCG 1 cut(s) 396
BshFI GGCC 5 cut(s) 482, 564, 629, 1058, 1094
BshNI GGYRCC 1 cut(s) 538
BshTI ACCGGT 1 cut(s) 925
BsiHKCI CYCGRG 1 cut(s) 1134
BsiSI CCGG 4 cut(s) 276, 420, 683, 926
BslFI GGGAC 2 cut(s) 136, 957
BslI CCNNNNNNNGG 7 cut(s) 275, 349, 419, 571, 592, 624, 973
BsmAI GTCTC 2 cut(s) 405, 513
BsmFI GGGAC 2 cut(s) 136, 957
BsnI GGCC 5 cut(s) 482, 564, 629, 1058, 1094
BsoBI CYCGRG 1 cut(s) 1134
Bsp1407I TGTACA 1 cut(s) 747
Bsp143I GATC 3 cut(s) 10, 37, 1111
BspACI CCGC 8 cut(s) 30, 124, 211, 237, 262, 394, 875, 1091
BspANI GGCC 5 cut(s) 482, 564, 629, 1058, 1094
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 2 cut(s) 540, 597
BspOI GCTAGC 1 cut(s) 477
BspPI GGATC 2 cut(s) 5, 1106
BspT107I GGYRCC 1 cut(s) 538
BsrBI CCGCTC 2 cut(s) 126, 213
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 3 cut(s) 419, 682, 925
BsrGI TGTACA 1 cut(s) 747
BsrI ACTGG 3 cut(s) 148, 766, 1008
BssAI RCCGGY 3 cut(s) 419, 682, 925
BssECI CCNNGG 2 cut(s) 908, 1044
BssMI GATC 3 cut(s) 10, 37, 1111
BssNAI GTATAC 1 cut(s) 410
BssT1I CCWWGG 1 cut(s) 1044
Bst1107I GTATAC 1 cut(s) 410
Bst2UI CCWGG 2 cut(s) 479, 631
Bst4CI ACNGT 2 cut(s) 490, 610
Bst6I CTCTTC 2 cut(s) 408, 798
BstAUI TGTACA 1 cut(s) 747
BstBAI YACGTR 1 cut(s) 470
BstC8I GCNNGC 4 cut(s) 283, 421, 475, 994
BstDEI CTNAG 1 cut(s) 1073
BstDSI CCRYGG 1 cut(s) 908
BstF5I GGATG 4 cut(s) 23, 119, 162, 838
BstFNI CGCG 1 cut(s) 396
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 3 cut(s) 13, 40, 1114
BstMAI GTCTC 2 cut(s) 405, 513
BstMBI GATC 3 cut(s) 10, 37, 1111
BstMWI GCNNNNNNNGC 4 cut(s) 449, 479, 998, 1055
BstNI CCWGG 2 cut(s) 479, 631
BstSCI CCNGG 2 cut(s) 477, 629
BstSFI CTRYAG 1 cut(s) 661
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 3 cut(s) 792, 795, 859
BstXI CCANNNNNNTGG 1 cut(s) 909
BstZ17I GTATAC 1 cut(s) 410
BsuRI GGCC 5 cut(s) 482, 564, 629, 1058, 1094
BtgI CCRYGG 1 cut(s) 908
BtsCI GGATG 4 cut(s) 23, 119, 162, 838
BtsI GCAGTG 2 cut(s) 902, 946
BtsIMutI CAGTG 3 cut(s) 615, 902, 946
Cac8I GCNNGC 4 cut(s) 283, 421, 475, 994
CfoI GCGC 1 cut(s) 649
Cfr10I RCCGGY 3 cut(s) 419, 682, 925
Cfr13I GGNCC 3 cut(s) 352, 615, 627
Csp6I GTAC 4 cut(s) 658, 748, 824, 1005
CspAI ACCGGT 1 cut(s) 925
CviAII CATG 3 cut(s) 224, 889, 1031
CviQI GTAC 4 cut(s) 658, 748, 824, 1005
DdeI CTNAG 1 cut(s) 1073
DpnI GATC 3 cut(s) 12, 39, 1113
DpnII GATC 3 cut(s) 10, 37, 1111
EaeI YGGCCR 2 cut(s) 1056, 1092
Eam1104I CTCTTC 2 cut(s) 408, 798
EarI CTCTTC 2 cut(s) 408, 798
EciI GGCGGA 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 1044
Eco147I AGGCCT 1 cut(s) 564
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 2 cut(s) 352, 615
Eco72I CACGTG 1 cut(s) 470
Eco88I CYCGRG 1 cut(s) 1134
EcoRI GAATTC 1 cut(s) 897
EcoRII CCWGG 2 cut(s) 477, 629
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 1 cut(s) 1044
EcoT22I ATGCAT 1 cut(s) 1032
ErhI CCWWGG 1 cut(s) 1044
FaeI CATG 3 cut(s) 227, 892, 1034
FaqI GGGAC 2 cut(s) 136, 957
FatI CATG 3 cut(s) 223, 888, 1030
FauI CCCGC 3 cut(s) 131, 218, 401
FblI GTMKAC 2 cut(s) 384, 409
Fnu4HI GCNGC 4 cut(s) 781, 784, 873, 1092
FokI GGATG 4 cut(s) 10, 106, 149, 845
Fsp4HI GCNGC 4 cut(s) 781, 784, 873, 1092
FspBI CTAG 3 cut(s) 474, 1062, 1150
GlaI GCGC 1 cut(s) 648
GluI GCNGC 4 cut(s) 781, 784, 873, 1092
GsaI CCCAGC 2 cut(s) 519, 591
HaeIII GGCC 5 cut(s) 482, 564, 629, 1058, 1094
HapII CCGG 4 cut(s) 276, 420, 683, 926
HhaI GCGC 1 cut(s) 649
Hin1II CATG 3 cut(s) 227, 892, 1034
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 2 cut(s) 272, 1066
HpaII CCGG 4 cut(s) 276, 420, 683, 926
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 4 cut(s) 385, 410, 493, 618
Hpy188I TCNGA 2 cut(s) 109, 331
Hpy188III TCNNGA 4 cut(s) 44, 734, 864, 1134
Hpy8I GTNNAC 4 cut(s) 385, 410, 493, 618
HpyAV CCTTC 7 cut(s) 192, 216, 266, 328, 365, 374, 848
HpyCH4III ACNGT 2 cut(s) 490, 610
HpyCH4IV ACGT 3 cut(s) 469, 495, 796
HpyCH4V TGCA 4 cut(s) 281, 688, 922, 1030
HpyF10VI GCNNNNNNNGC 4 cut(s) 449, 479, 998, 1055
HpyF3I CTNAG 1 cut(s) 1073
HpySE526I ACGT 3 cut(s) 469, 495, 796
Hsp92II CATG 3 cut(s) 227, 892, 1034
HspAI GCGC 1 cut(s) 647
KroI GCCGGC 1 cut(s) 419
KroNI GCCGGC 1 cut(s) 421
Kzo9I GATC 3 cut(s) 10, 37, 1111
LmnI GCTCC 3 cut(s) 131, 218, 976
Lsp1109I GCAGC 3 cut(s) 792, 795, 859
MaeI CTAG 3 cut(s) 474, 1062, 1150
MaeII ACGT 3 cut(s) 469, 495, 796
MalI GATC 3 cut(s) 12, 39, 1113
MbiI CCGCTC 2 cut(s) 126, 213
MboI GATC 3 cut(s) 10, 37, 1111
MboII GAAGA 3 cut(s) 47, 395, 815
MlsI TGGCCA 1 cut(s) 1058
MluCI AATT 7 cut(s) 87, 553, 701, 897, 914, 936, 1122
MluNI TGGCCA 1 cut(s) 1058
MlyI GAGTC 2 cut(s) 266, 1075
MmeI TCCRAC 4 cut(s) 225, 267, 367, 1139
Mox20I TGGCCA 1 cut(s) 1058
Mph1103I ATGCAT 1 cut(s) 1032
MroNI GCCGGC 1 cut(s) 419
MscI TGGCCA 1 cut(s) 1058
MseI TTAA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 907
Msp20I TGGCCA 1 cut(s) 1058
MspI CCGG 4 cut(s) 276, 420, 683, 926
MspR9I CCNGG 2 cut(s) 479, 631
MvaI CCWGG 2 cut(s) 479, 631
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 4 cut(s) 449, 479, 998, 1055
NaeI GCCGGC 1 cut(s) 421
NdeII GATC 3 cut(s) 10, 37, 1111
NgoMIV GCCGGC 1 cut(s) 419
NheI GCTAGC 1 cut(s) 473
NlaIII CATG 3 cut(s) 227, 892, 1034
NlaIV GGNNCC 2 cut(s) 540, 597
NsiI ATGCAT 1 cut(s) 1032
OliI CACNNNNGTG 1 cut(s) 907
PaeR7I CTCGAG 1 cut(s) 1134
PceI AGGCCT 1 cut(s) 564
PdiI GCCGGC 1 cut(s) 421
PflMI CCANNNNNTGG 1 cut(s) 349
PinAI ACCGGT 1 cut(s) 925
PkrI GCNGC 4 cut(s) 782, 785, 874, 1093
PleI GAGTC 2 cut(s) 266, 1074
PmaCI CACGTG 1 cut(s) 470
PmlI CACGTG 1 cut(s) 470
PpsI GAGTC 2 cut(s) 266, 1074
Ppu21I YACGTR 1 cut(s) 470
Psp6I CCWGG 2 cut(s) 477, 629
PspCI CACGTG 1 cut(s) 470
PspFI CCCAGC 2 cut(s) 515, 587
PspGI CCWGG 2 cut(s) 477, 629
PspN4I GGNNCC 2 cut(s) 540, 597
PspPI GGNCC 3 cut(s) 352, 615, 627
RsaI GTAC 4 cut(s) 659, 749, 825, 1006
RsaNI GTAC 4 cut(s) 658, 748, 824, 1005
RseI CAYNNNNRTG 1 cut(s) 907
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 4 cut(s) 781, 784, 873, 1092
Sau3AI GATC 3 cut(s) 10, 37, 1111
Sau96I GGNCC 3 cut(s) 352, 615, 627
SchI GAGTC 2 cut(s) 266, 1075
ScrFI CCNGG 2 cut(s) 479, 631
SfcI CTRYAG 1 cut(s) 661
Sfr274I CTCGAG 1 cut(s) 1134
SinI GGWCC 2 cut(s) 352, 615
SlaI CTCGAG 1 cut(s) 1134
SmiMI CAYNNNNRTG 1 cut(s) 907
SmlI CTYRAG 2 cut(s) 650, 1134
SmoI CTYRAG 2 cut(s) 650, 1134
Sse9I AATT 7 cut(s) 87, 553, 701, 897, 914, 936, 1122
SseBI AGGCCT 1 cut(s) 564
SsiI CCGC 8 cut(s) 30, 124, 211, 237, 262, 394, 875, 1091
SspMI CTAG 3 cut(s) 474, 1062, 1150
StuI AGGCCT 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 477, 629
StyI CCWWGG 1 cut(s) 1044
TaaI ACNGT 2 cut(s) 490, 610
TaiI ACGT 3 cut(s) 472, 498, 799
TaqI TCGA 4 cut(s) 5, 557, 699, 1135
TasI AATT 7 cut(s) 87, 553, 701, 897, 914, 936, 1122
TatI WGTACW 3 cut(s) 657, 747, 823
TauI GCSGC 1 cut(s) 1094
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 3 cut(s) 615, 909, 953
TseI GCWGC 3 cut(s) 780, 783, 872
TspRI CASTG 3 cut(s) 615, 909, 953
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 352, 615
XapI RAATTY 4 cut(s) 701, 897, 936, 1122
XcmI CCANNNNNNNNNTGG 1 cut(s) 1051
XhoI CTCGAG 1 cut(s) 1134
XmiI GTMKAC 2 cut(s) 384, 409
XspI CTAG 3 cut(s) 474, 1062, 1150
Zsp2I ATGCAT 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.