RLG00000002247

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015440
Physical Location & Seq
Reverse (-)
30554112 .. 30555254
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002247

Sequence Viewer

Length: 1143 bp
ATGTCTAACGATCCCTCCACCACCACCTCATCCATTGCTGGAGACACCACCACGGCAACCACAGACGATACCATCAGCACCGCCACCACCATCCCAGAAGACACCACCAGTTCGATCGCCCTAACCTCCACCACCAAAGCCTCAACCTCATTAGACTCCTCCTCATCCTCAACCTCCTCCAAGCCCCAAGCAGCAGCAATCCAATCCCTCTCCGCCATCCTCCCCTCCCTCTCCTCCACCTCCCACACCACCGCCCGTTCCCTCCTCCACGACCCCCACACATCCTCCCAAATCTCCTCCCTCCTCAAACAACCCGACTCTGGCGCCGGCGACAACAACCTCTGCCGCTGGCTCTACGACACCTTCCAGTCCTCCGATCCCGACCTCCAGCTCCTCGTCCTCCGCTTCCTCCCCCTCGTCGCTGGTCTCTACCTCTCCCGCGTCCCCCTCCGCATCCCCCTCGCCGGCTTCGAGGCCGTCCTCTTGGCCCTCTACGCTCACGAGACCGCCTCCCGCGACGGCCATTCCGTAACCGTCAACGTTCCCGACGTGTCCCACCCCAGCCTCTACCACGAAACCAAGGCCGCCGCCGTCAAGAGCAACGCCACCGAGCTCAACTTGGCCGTTATATCACCCAGCTTGGAACCTCACGGCACCGTCAGATCCACACGGAGGGCTAGGATCGTCGGGGTGGCGCTGGAATTGTACTACACCAAAATATACGAAATGCCAGTCGAGTCCAAGATCGATTTCTGCGAGTTTTGTAAGGTTTGGGCTGGTCAGGACGGAGAAATGTACAAACAGGACGTGGAGAAGAGTAGTGGAAACAAAATAAACGAGGGAAAGGAGGAGGGCAGTTTGGGGAATGGGATGGGGAGGGTTCCCTTGCCATGCGAATTGCTGCAACCAGTTTTGAGGATTTTAGGGCACTGCCTTTTGTGCCCTAATCAGAACAAGAAGCTCACAGATAAGGCGAGCGAAGCGTGTAGGAGTCTGTATGCGAGGTCCATGCATGATATTAATCCCAAGGCAATTTTGGCTACTGGGAGTTTGCTTAGGCTAGCTAACATGGCTTTCGACTCCACCAAGCAAGACCTTTTTGATCCAACTGACGTTCCCAAATCTAGAGTCATCTCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

381

Amino Acids

40.86

Weight (kDa)

5.94

Isoelectric Point (pI)

54.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hyccin PF09790 81 - 359 4e-47 Hyccin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G21050
fragaria_vesca FvH4_5g28070
malus_domestica MD11G1179900.v1.1
prunus_persica Prupe.6G113000_v2.0.a1 Prupe.6G152400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220241
rosa_laevigata RLG00000002247
rosa_multiflora Rmu_co8248515.1_g000001 Rmu_sc0001136.1_g000021
rosa_roxburghii Rroxscaffold_3G00239690
rosa_rugosa Rorug07G0191400
rosa_samantha Rh7AG333500 Rh7BG323700 Rh7CG351100 Rh7DG329800
rosa_wichuraiana Rw7G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 323, 653
AccII CGCG 2 cut(s) 441, 516
AclI AACGTT 1 cut(s) 540
AclWI GGATC 5 cut(s) 5, 371, 657, 689, 1097
AcoI YGGCCR 2 cut(s) 520, 621
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 2 cut(s) 707, 797
AfiI CCNNNNNNNGG 3 cut(s) 320, 464, 672
AflIII ACRYGT 1 cut(s) 549
AjiI CACGTC 2 cut(s) 550, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AluBI AGCT 5 cut(s) 391, 613, 639, 961, 1064
AluI AGCT 5 cut(s) 391, 613, 639, 961, 1064
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 3 cut(s) 36, 431, 497
AlwI GGATC 5 cut(s) 5, 371, 657, 689, 1097
AoxI GGCC 5 cut(s) 474, 486, 520, 582, 621
ApeKI GCWGC 3 cut(s) 191, 194, 901
ArsI GACNNNNNNTTYG 3 cut(s) 717, 749, 1113
AseI ATTAAT 1 cut(s) 1020
AspLEI GCGC 2 cut(s) 326, 697
AspS9I GGNCC 2 cut(s) 487, 1005
AsuHPI GGTGA 1 cut(s) 624
AsuNHI GCTAGC 1 cut(s) 1060
AvaII GGWCC 1 cut(s) 1005
BaeGI GKGCMC 2 cut(s) 930, 944
BanI GGYRCC 2 cut(s) 323, 653
BanII GRGCYC 1 cut(s) 615
BauI CACGAG 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 615
BbvI GCAGC 3 cut(s) 203, 206, 888
BccI CCATC 4 cut(s) 80, 98, 224, 865
BceAI ACGGC 6 cut(s) 69, 461, 535, 575, 608, 667
BcgI CGANNNNNNTGC 4 cut(s) 442, 476, 991, 1025
BcoDI GTCTC 3 cut(s) 36, 431, 497
BfaI CTAG 3 cut(s) 678, 1061, 1125
BfoI RGCGCY 2 cut(s) 327, 698
BisI GCNGC 6 cut(s) 192, 195, 346, 585, 588, 902
BlsI GCNGC 6 cut(s) 193, 196, 347, 586, 589, 903
Bme18I GGWCC 1 cut(s) 1005
BmgBI CACGTC 2 cut(s) 550, 808
BmgT120I GGNCC 2 cut(s) 487, 1005
BmiI GGNNCC 4 cut(s) 325, 645, 655, 882
BmrI ACTGGG 1 cut(s) 1053
BmsI GCATC 1 cut(s) 462
BmtI GCTAGC 1 cut(s) 1064
BmuI ACTGGG 1 cut(s) 1053
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 2 cut(s) 60, 371
Bpu10I CCTNAGC 1 cut(s) 1055
Bsa29I ATCGAT 1 cut(s) 747
BsaBI GATNNNNATC 1 cut(s) 1020
BsaHI GRCGYC 1 cut(s) 324
BsaI GGTCTC 2 cut(s) 431, 497
BsaJI CCNNGG 3 cut(s) 51, 579, 1026
BsaXI ACNNNNNCTCC 3 cut(s) 29, 269, 299
Bsc4I CCNNNNNNNGG 3 cut(s) 320, 464, 672
Bse118I RCCGGY 2 cut(s) 326, 464
Bse1I ACTGG 5 cut(s) 108, 367, 731, 908, 1048
Bse3DI GCAATG 1 cut(s) 33
Bse8I GATNNNNATC 1 cut(s) 1020
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 3 cut(s) 51, 579, 1026
BseGI GGATG 7 cut(s) 29, 90, 164, 216, 281, 453, 876
BseJI GATNNNNATC 1 cut(s) 1020
BseLI CCNNNNNNNGG 3 cut(s) 320, 464, 672
BseMI GCAATG 1 cut(s) 33
BseNI ACTGG 5 cut(s) 108, 367, 731, 908, 1048
BseRI GAGGAG 9 cut(s) 148, 151, 166, 223, 254, 286, 293, 383, 863
BseSI GKGCMC 2 cut(s) 930, 944
BseXI GCAGC 3 cut(s) 203, 206, 888
BseYI CCCAGC 2 cut(s) 560, 635
Bsh1236I CGCG 2 cut(s) 441, 516
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 5 cut(s) 476, 488, 522, 584, 623
BshNI GGYRCC 2 cut(s) 323, 653
BshVI ATCGAT 1 cut(s) 747
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 615
BsiSI CCGG 2 cut(s) 327, 465
BslFI GGGAC 2 cut(s) 428, 538
BslI CCNNNNNNNGG 3 cut(s) 320, 464, 672
BsmAI GTCTC 3 cut(s) 36, 431, 497
BsmFI GGGAC 2 cut(s) 428, 538
BsnI GGCC 5 cut(s) 476, 488, 522, 584, 623
Bso31I GGTCTC 2 cut(s) 431, 497
Bsp1286I GDGCHC 3 cut(s) 615, 930, 944
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 7 cut(s) 10, 114, 376, 662, 681, 744, 1102
BspANI GGCC 5 cut(s) 476, 488, 522, 584, 623
BspDI ATCGAT 1 cut(s) 747
BspFNI CGCG 2 cut(s) 441, 516
BspLI GGNNCC 4 cut(s) 325, 645, 655, 882
BspOI GCTAGC 1 cut(s) 1064
BspPI GGATC 5 cut(s) 5, 371, 657, 689, 1097
BspT107I GGYRCC 2 cut(s) 323, 653
BspTNI GGTCTC 2 cut(s) 431, 497
BsrDI GCAATG 1 cut(s) 33
BsrFI RCCGGY 2 cut(s) 326, 464
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 5 cut(s) 108, 367, 731, 908, 1048
BssAI RCCGGY 2 cut(s) 326, 464
BssECI CCNNGG 3 cut(s) 51, 579, 1026
BssMI GATC 7 cut(s) 10, 114, 376, 662, 681, 744, 1102
BssNI GRCGYC 1 cut(s) 324
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 2 cut(s) 579, 1026
Bst2BI CACGAG 1 cut(s) 500
Bst4CI ACNGT 2 cut(s) 535, 658
Bst6I CTCTTC 1 cut(s) 809
BstACI GRCGYC 1 cut(s) 324
BstAUI TGTACA 1 cut(s) 795
BstC8I GCNNGC 5 cut(s) 328, 350, 466, 976, 1062
BstDEI CTNAG 1 cut(s) 1055
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 7 cut(s) 29, 90, 164, 216, 281, 453, 876
BstFNI CGCG 2 cut(s) 441, 516
BstH2I RGCGCY 2 cut(s) 327, 698
BstHHI GCGC 2 cut(s) 326, 697
BstKTI GATC 7 cut(s) 13, 117, 379, 665, 684, 747, 1105
BstMAI GTCTC 3 cut(s) 36, 431, 497
BstMBI GATC 7 cut(s) 10, 114, 376, 662, 681, 744, 1102
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 5 cut(s) 494, 939, 980, 1037, 1070
BstSLI GKGCMC 2 cut(s) 930, 944
BstUI CGCG 2 cut(s) 441, 516
BstV1I GCAGC 3 cut(s) 203, 206, 888
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
Bsu15I ATCGAT 1 cut(s) 747
BsuRI GGCC 5 cut(s) 476, 488, 522, 584, 623
BsuTUI ATCGAT 1 cut(s) 747
BtgI CCRYGG 1 cut(s) 51
BtrI CACGTC 2 cut(s) 550, 808
BtsCI GGATG 7 cut(s) 29, 90, 164, 216, 281, 453, 876
BtsI GCAGTG 1 cut(s) 928
BtsIMutI CAGTG 1 cut(s) 928
Cac8I GCNNGC 5 cut(s) 328, 350, 466, 976, 1062
CfoI GCGC 2 cut(s) 326, 697
Cfr10I RCCGGY 2 cut(s) 326, 464
Cfr13I GGNCC 2 cut(s) 487, 1005
ClaI ATCGAT 1 cut(s) 747
CseI GACGC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 706, 796
CviAII CATG 4 cut(s) 891, 1009, 1013, 1069
CviQI GTAC 2 cut(s) 706, 796
DdeI CTNAG 1 cut(s) 1055
DinI GGCGCC 1 cut(s) 325
DpnI GATC 7 cut(s) 12, 116, 378, 664, 683, 746, 1104
DpnII GATC 7 cut(s) 10, 114, 376, 662, 681, 744, 1102
EaeI YGGCCR 2 cut(s) 520, 621
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
EciI GGCGGA 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 613
Eco130I CCWWGG 2 cut(s) 579, 1026
Eco24I GRGCYC 1 cut(s) 615
Eco31I GGTCTC 2 cut(s) 431, 497
Eco47I GGWCC 1 cut(s) 1005
Eco53kI GAGCTC 1 cut(s) 613
EcoICRI GAGCTC 1 cut(s) 613
EcoT14I CCWWGG 2 cut(s) 579, 1026
EcoT22I ATGCAT 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 615
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 579, 1026
FaeI CATG 4 cut(s) 894, 1012, 1016, 1072
FaiI YATR 8 cut(s) 629, 721, 892, 999, 1010, 1014, 1070, 1139
FaqI GGGAC 2 cut(s) 428, 538
FatI CATG 4 cut(s) 890, 1008, 1012, 1068
FauI CCCGC 2 cut(s) 446, 521
Fnu4HI GCNGC 6 cut(s) 192, 195, 346, 585, 588, 902
FokI GGATG 7 cut(s) 16, 77, 151, 203, 268, 440, 883
FriOI GRGCYC 1 cut(s) 615
Fsp4HI GCNGC 6 cut(s) 192, 195, 346, 585, 588, 902
FspBI CTAG 3 cut(s) 678, 1061, 1125
GlaI GCGC 2 cut(s) 325, 696
GluI GCNGC 6 cut(s) 192, 195, 346, 585, 588, 902
GsaI CCCAGC 2 cut(s) 564, 639
GsuI CTGGAG 2 cut(s) 60, 371
HaeII RGCGCY 2 cut(s) 327, 698
HaeIII GGCC 5 cut(s) 476, 488, 522, 584, 623
HapII CCGG 2 cut(s) 327, 465
HgaI GACGC 1 cut(s) 430
HhaI GCGC 2 cut(s) 326, 697
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 4 cut(s) 894, 1012, 1016, 1072
Hin6I GCGC 2 cut(s) 324, 695
HinP1I GCGC 2 cut(s) 324, 695
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 6 cut(s) 155, 317, 737, 991, 1079, 1128
HpaII CCGG 2 cut(s) 327, 465
HphI GGTGA 1 cut(s) 624
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 3 cut(s) 376, 662, 951
Hpy188III TCNNGA 6 cut(s) 380, 500, 545, 595, 782, 1125
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 4 cut(s) 422, 521, 551, 689
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 2 cut(s) 535, 658
HpyCH4IV ACGT 4 cut(s) 540, 549, 807, 1113
HpyCH4V TGCA 2 cut(s) 904, 1012
HpyF10VI GCNNNNNNNGC 5 cut(s) 494, 939, 980, 1037, 1070
HpyF3I CTNAG 1 cut(s) 1055
HpySE526I ACGT 4 cut(s) 540, 549, 807, 1113
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 4 cut(s) 894, 1012, 1016, 1072
HspAI GCGC 2 cut(s) 324, 695
KasI GGCGCC 1 cut(s) 323
KroI GCCGGC 2 cut(s) 326, 464
KroNI GCCGGC 2 cut(s) 328, 466
Kzo9I GATC 7 cut(s) 10, 114, 376, 662, 681, 744, 1102
LmnI GCTCC 1 cut(s) 396
Lsp1109I GCAGC 3 cut(s) 203, 206, 888
LweI GCATC 1 cut(s) 462
MaeI CTAG 3 cut(s) 678, 1061, 1125
MaeII ACGT 4 cut(s) 540, 549, 807, 1113
MaeIII GTNAC 1 cut(s) 529
MalI GATC 7 cut(s) 12, 116, 378, 664, 683, 746, 1104
MboI GATC 7 cut(s) 10, 114, 376, 662, 681, 744, 1102
MboII GAAGA 2 cut(s) 110, 826
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 3 cut(s) 615, 930, 944
MluCI AATT 3 cut(s) 701, 896, 1032
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 6 cut(s) 149, 311, 746, 1000, 1073, 1137
MmeI TCCRAC 1 cut(s) 1130
Mph1103I ATGCAT 1 cut(s) 1014
MreI CGCCGGCG 1 cut(s) 326
MroNI GCCGGC 2 cut(s) 326, 464
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 1 cut(s) 1136
MspA1I CMGCKG 1 cut(s) 348
MspI CCGG 2 cut(s) 327, 465
MvnI CGCG 2 cut(s) 441, 516
MwoI GCNNNNNNNGC 5 cut(s) 494, 939, 980, 1037, 1070
NaeI GCCGGC 2 cut(s) 328, 466
NarI GGCGCC 1 cut(s) 324
NdeII GATC 7 cut(s) 10, 114, 376, 662, 681, 744, 1102
NgoMIV GCCGGC 2 cut(s) 326, 464
NheI GCTAGC 1 cut(s) 1060
NlaIII CATG 4 cut(s) 894, 1012, 1016, 1072
NlaIV GGNNCC 4 cut(s) 325, 645, 655, 882
NsiI ATGCAT 1 cut(s) 1014
PcsI WCGNNNNNNNCGW 4 cut(s) 468, 525, 546, 753
PdiI GCCGGC 2 cut(s) 328, 466
PkrI GCNGC 6 cut(s) 193, 196, 347, 586, 589, 903
Ple19I CGATCG 1 cut(s) 117
PleI GAGTC 6 cut(s) 149, 311, 745, 999, 1073, 1136
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 6 cut(s) 149, 311, 745, 999, 1073, 1136
PshBI ATTAAT 1 cut(s) 1020
Psp124BI GAGCTC 1 cut(s) 615
Psp1406I AACGTT 1 cut(s) 540
PspFI CCCAGC 2 cut(s) 560, 635
PspN4I GGNNCC 4 cut(s) 325, 645, 655, 882
PspPI GGNCC 2 cut(s) 487, 1005
PsuI RGATCY 1 cut(s) 662
PvuI CGATCG 1 cut(s) 117
RsaI GTAC 2 cut(s) 707, 797
RsaNI GTAC 2 cut(s) 706, 796
RseI CAYNNNNRTG 1 cut(s) 1136
SacI GAGCTC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 6 cut(s) 192, 195, 346, 585, 588, 902
Sau3AI GATC 7 cut(s) 10, 114, 376, 662, 681, 744, 1102
Sau96I GGNCC 2 cut(s) 487, 1005
SchI GAGTC 6 cut(s) 149, 311, 746, 1000, 1073, 1137
SduI GDGCHC 3 cut(s) 615, 930, 944
SfaNI GCATC 1 cut(s) 462
SfoI GGCGCC 1 cut(s) 325
SgrAI CRCCGGYG 1 cut(s) 326
SinI GGWCC 1 cut(s) 1005
SmiMI CAYNNNNRTG 1 cut(s) 1136
Sse9I AATT 3 cut(s) 701, 896, 1032
SspDI GGCGCC 1 cut(s) 323
SspMI CTAG 3 cut(s) 678, 1061, 1125
SstI GAGCTC 1 cut(s) 615
StyI CCWWGG 2 cut(s) 579, 1026
TaaI ACNGT 2 cut(s) 535, 658
TaiI ACGT 4 cut(s) 543, 552, 810, 1116
TaqI TCGA 5 cut(s) 113, 471, 735, 747, 1077
TasI AATT 3 cut(s) 701, 896, 1032
TatI WGTACW 2 cut(s) 705, 795
TauI GCSGC 3 cut(s) 348, 587, 590
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 1 cut(s) 935
TseI GCWGC 3 cut(s) 191, 194, 901
TspGWI ACGGA 3 cut(s) 517, 685, 801
TspRI CASTG 1 cut(s) 935
VpaK11BI GGWCC 1 cut(s) 1005
VspI ATTAAT 1 cut(s) 1020
XbaI TCTAGA 1 cut(s) 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 1033
XspI CTAG 3 cut(s) 678, 1061, 1125
Zsp2I ATGCAT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.