Prupe.6G152400_v2.0.a1

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0015440
Physical Location & Seq
Reverse (-)
13258945 .. 13260447
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G152400.1

Sequence Viewer

Length: 1101 bp
ATGTCCAACGACCCCTCCACTTCATCCATCCTAGAAGATGATCAAGATACCATCAATTCCACCACCACCTCCACCATCACCCCAACCCCAACAACTACCACTTCAGCCTCAGCCTCAGCTTCATCACATAACTCATCCCTCTCCAAATGCCAATCAGCAGTTCAATCCCTCTCAACCATTCTCCCCTCTCTCCCTCCTTCCCTCTCCTCCTCCTCCAACCCCGCCCACACCCTCCTCCATGACCACCACACAGCCTCCCACATCTCTTCCCTTCTCCGCCAACCCGACTCCGGCGCAGGCGACAACAACCTCTGCCGCTGGCTCTACGACACCTTCCAATCCTCCGATCCCGACCTCCAACTCCTCGTCCTTCGCTTCCTTCCCATCATCGCGGGCATCTACATCTCCCGTGCTGCCCTCCGGGTACCCCTAGCTGGCTTTGAGGCAGTCCTCTTAGCCCTCTACGCCCACGAAACCGCAACACGTTCTGGCCACTCCATAACCGTCAGTGTCCCCGACTTGTCTCATCCCAGTCTCTACCACGAAAGCAAAGCACCCACTAAAAACAATTCCACAGGCCTAAATTTGGCTGTCATATCTCCAAGTTTGGAACCCTATGGTACAGTGAGGTCCACAAGGAGGGCTAGGATTGTTGGGGTGGCACTGGAGTTGTACTACAGCAAGATACATGAAATGCCGGTGCAATCAAAGATTGAATTTTGCGAGTTTTGCAGGGTTTGGGCTGGCCAAGACGGGGAAATGTACAAGGAGATGACTGGTAGTAGCTGTAGCAGTAGGAGTGAAAGCAAAGAAGAGGATGGAAATGGAGAAAAGGCGGGGAGCATTCCTTTGCCATGGGAATTAATGCAACCGGTTTTCAGGATTTTGGGTCACTGCCTTTTGTGTCCAAACCAAAACAAGGAGTTGGTTGATAAGGGCAGTGAGGCTTGTAGGAGTTTGTATGCCAGGTCTATGCATGATATCAATCCCAAAGCTATTTTGGTCACTGGGAGTCTTCTTAGGCTCACTAAGCAAGCATTGGACACTAACGATGATTTTGATCCAACTGAGATTCCTAAATCTCGAATTATTAGTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

367

Amino Acids

39.75

Weight (kDa)

5.89

Isoelectric Point (pI)

60.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G21050
fragaria_vesca FvH4_5g28070
malus_domestica MD11G1179900.v1.1
prunus_persica Prupe.6G113000_v2.0.a1 Prupe.6G152400_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0220241
rosa_laevigata RLG00000002247
rosa_multiflora Rmu_co8248515.1_g000001 Rmu_sc0001136.1_g000021
rosa_roxburghii Rroxscaffold_3G00239690
rosa_rugosa Rorug07G0191400
rosa_samantha Rh7AG333500 Rh7BG323700 Rh7CG351100 Rh7DG329800
rosa_wichuraiana Rw7G028130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 424
AccB1I GGYRCC 1 cut(s) 424
AccII CGCG 1 cut(s) 392
AciI CCGC 6 cut(s) 222, 277, 316, 392, 477, 836
AclWI GGATC 2 cut(s) 341, 1055
AcoI YGGCCR 2 cut(s) 490, 745
AcsI RAATTY 2 cut(s) 583, 716
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 4 cut(s) 426, 622, 674, 764
AfiI CCNNNNNNNGG 6 cut(s) 290, 434, 586, 639, 754, 919
AflIII ACRYGT 1 cut(s) 482
AgeI ACCGGT 1 cut(s) 871
AgsI TTSAA 2 cut(s) 164, 716
AjnI CCWGG 1 cut(s) 965
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AluBI AGCT 4 cut(s) 119, 434, 786, 995
AluI AGCT 4 cut(s) 119, 434, 786, 995
Alw26I GTCTC 2 cut(s) 528, 539
AlwI GGATC 2 cut(s) 341, 1055
AoxI GGCC 3 cut(s) 490, 577, 745
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 2 cut(s) 583, 716
AseI ATTAAT 1 cut(s) 863
AsiGI ACCGGT 1 cut(s) 871
Asp718I GGTACC 1 cut(s) 424
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 630
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 1 cut(s) 70
AvaII GGWCC 1 cut(s) 630
BalI TGGCCA 2 cut(s) 492, 747
BanI GGYRCC 1 cut(s) 424
BbsI GAAGAC 1 cut(s) 1007
BbvCI CCTCAGC 2 cut(s) 109, 115
BbvI GCAGC 1 cut(s) 400
BccI CCATC 5 cut(s) 35, 59, 83, 392, 812
BciT130I CCWGG 1 cut(s) 967
BclI TGATCA 1 cut(s) 40
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 528, 539
BfaI CTAG 4 cut(s) 32, 431, 645, 1099
BfmI CTRYAG 2 cut(s) 676, 787
BisI GCNGC 2 cut(s) 316, 414
BlsI GCNGC 2 cut(s) 317, 415
Bme1390I CCNGG 2 cut(s) 422, 967
Bme18I GGWCC 1 cut(s) 630
BmgT120I GGNCC 1 cut(s) 630
BmiI GGNNCC 2 cut(s) 426, 612
BmrFI CCNGG 2 cut(s) 422, 967
BmrI ACTGGG 2 cut(s) 525, 1017
BmsI GCATC 1 cut(s) 405
BmuI ACTGGG 2 cut(s) 525, 1017
BpiI GAAGAC 1 cut(s) 1007
BpmI CTGGAG 1 cut(s) 686
Bpu10I CCTNAGC 2 cut(s) 109, 115
BpuMI CCSGG 1 cut(s) 422
BsaBI GATNNNNATC 2 cut(s) 984, 1059
BsaJI CCNNGG 1 cut(s) 854
BsaWI WCCGGW 1 cut(s) 871
BsaXI ACNNNNNCTCC 3 cut(s) 29, 239, 269
Bsc4I CCNNNNNNNGG 6 cut(s) 290, 434, 586, 639, 754, 919
Bse118I RCCGGY 2 cut(s) 697, 871
Bse1I ACTGG 4 cut(s) 531, 669, 781, 1012
Bse8I GATNNNNATC 2 cut(s) 984, 1059
BseBI CCWGG 1 cut(s) 967
BseDI CCNNGG 1 cut(s) 854
BseGI GGATG 5 cut(s) 23, 27, 134, 526, 823
BseJI GATNNNNATC 2 cut(s) 984, 1059
BseLI CCNNNNNNNGG 6 cut(s) 290, 434, 586, 639, 754, 919
BseMII CTCAG 3 cut(s) 123, 129, 1059
BseNI ACTGG 4 cut(s) 531, 669, 781, 1012
BseRI GAGGAG 5 cut(s) 196, 199, 202, 224, 353
BseXI GCAGC 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 392
BshFI GGCC 3 cut(s) 492, 579, 747
BshNI GGYRCC 1 cut(s) 424
BshTI ACCGGT 1 cut(s) 871
BsiSI CCGG 4 cut(s) 291, 421, 698, 872
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 6 cut(s) 290, 434, 586, 639, 754, 919
BsmAI GTCTC 2 cut(s) 528, 539
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 843
BsnI GGCC 3 cut(s) 492, 579, 747
Bsp1407I TGTACA 1 cut(s) 762
Bsp143I GATC 3 cut(s) 40, 346, 1060
Bsp19I CCATGG 1 cut(s) 854
BspACI CCGC 6 cut(s) 222, 277, 316, 392, 477, 836
BspANI GGCC 3 cut(s) 492, 579, 747
BspCNI CTCAG 3 cut(s) 122, 128, 1060
BspFNI CGCG 1 cut(s) 392
BspLI GGNNCC 2 cut(s) 426, 612
BspPI GGATC 2 cut(s) 341, 1055
BspT107I GGYRCC 1 cut(s) 424
BsrFI RCCGGY 2 cut(s) 697, 871
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 4 cut(s) 531, 669, 781, 1012
BssAI RCCGGY 2 cut(s) 697, 871
BssECI CCNNGG 1 cut(s) 854
BssMI GATC 3 cut(s) 40, 346, 1060
BssT1I CCWWGG 1 cut(s) 854
Bst2UI CCWGG 1 cut(s) 967
Bst4CI ACNGT 2 cut(s) 505, 625
Bst6I CTCTTC 2 cut(s) 271, 807
BstAUI TGTACA 1 cut(s) 762
BstC8I GCNNGC 6 cut(s) 298, 320, 394, 436, 745, 1035
BstDEI CTNAG 6 cut(s) 109, 115, 454, 1019, 1029, 1068
BstDSI CCRYGG 1 cut(s) 854
BstF5I GGATG 5 cut(s) 23, 27, 134, 526, 823
BstFNI CGCG 1 cut(s) 392
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 3 cut(s) 43, 349, 1063
BstMAI GTCTC 2 cut(s) 528, 539
BstMBI GATC 3 cut(s) 40, 346, 1060
BstMWI GCNNNNNNNGC 3 cut(s) 464, 729, 1030
BstNI CCWGG 1 cut(s) 967
BstSCI CCNGG 2 cut(s) 420, 965
BstSFI CTRYAG 2 cut(s) 676, 787
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 1 cut(s) 400
BstV2I GAAGAC 1 cut(s) 1007
BsuRI GGCC 3 cut(s) 492, 579, 747
BtgI CCRYGG 1 cut(s) 854
BtgZI GCGATG 1 cut(s) 373
BtsCI GGATG 5 cut(s) 23, 27, 134, 526, 823
BtsI GCAGTG 2 cut(s) 892, 946
BtsIMutI CAGTG 6 cut(s) 514, 630, 662, 892, 946, 1005
Cac8I GCNNGC 6 cut(s) 298, 320, 394, 436, 745, 1035
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 2 cut(s) 697, 871
Cfr13I GGNCC 1 cut(s) 630
Csp6I GTAC 4 cut(s) 425, 621, 673, 763
CspAI ACCGGT 1 cut(s) 871
CviAII CATG 4 cut(s) 239, 689, 855, 977
CviQI GTAC 4 cut(s) 425, 621, 673, 763
DdeI CTNAG 6 cut(s) 109, 115, 454, 1019, 1029, 1068
DpnI GATC 3 cut(s) 42, 348, 1062
DpnII GATC 3 cut(s) 40, 346, 1060
EaeI YGGCCR 2 cut(s) 490, 745
Eam1104I CTCTTC 2 cut(s) 271, 807
EarI CTCTTC 2 cut(s) 271, 807
EciI GGCGGA 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 854
Eco147I AGGCCT 1 cut(s) 579
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 1 cut(s) 630
Eco57I CTGAAG 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 965
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 1 cut(s) 854
EcoT22I ATGCAT 1 cut(s) 978
ErhI CCWWGG 1 cut(s) 854
FaeI CATG 4 cut(s) 242, 692, 858, 980
FaqI GGGAC 1 cut(s) 497
FatI CATG 4 cut(s) 238, 688, 854, 976
FauI CCCGC 3 cut(s) 229, 385, 829
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 2 cut(s) 316, 414
FokI GGATG 5 cut(s) 10, 14, 121, 513, 830
Fsp4HI GCNGC 2 cut(s) 316, 414
FspBI CTAG 4 cut(s) 32, 431, 645, 1099
GlaI GCGC 1 cut(s) 295
GluI GCNGC 2 cut(s) 316, 414
GsuI CTGGAG 1 cut(s) 686
HaeIII GGCC 3 cut(s) 492, 579, 747
HapII CCGG 4 cut(s) 291, 421, 698, 872
HhaI GCGC 1 cut(s) 296
Hin1II CATG 4 cut(s) 242, 692, 858, 980
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HinfI GANTC 3 cut(s) 287, 1012, 1072
HpaII CCGG 4 cut(s) 291, 421, 698, 872
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 1 cut(s) 633
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 4 cut(s) 44, 350, 880, 1083
Hpy8I GTNNAC 1 cut(s) 633
HpyAV CCTTC 5 cut(s) 207, 281, 343, 380, 389
HpyCH4III ACNGT 2 cut(s) 505, 625
HpyCH4IV ACGT 1 cut(s) 484
HpyCH4V TGCA 4 cut(s) 703, 732, 868, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 464, 729, 1030
HpyF3I CTNAG 6 cut(s) 109, 115, 454, 1019, 1029, 1068
HpySE526I ACGT 1 cut(s) 484
Hsp92II CATG 4 cut(s) 242, 692, 858, 980
HspAI GCGC 1 cut(s) 294
KpnI GGTACC 1 cut(s) 428
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 3 cut(s) 40, 346, 1060
LmnI GCTCC 1 cut(s) 840
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 1 cut(s) 405
MaeI CTAG 4 cut(s) 32, 431, 645, 1099
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 2 cut(s) 890, 1003
MalI GATC 3 cut(s) 42, 348, 1062
MboI GATC 3 cut(s) 40, 346, 1060
MboII GAAGA 4 cut(s) 47, 258, 824, 1007
MlsI TGGCCA 2 cut(s) 492, 747
MluCI AATT 6 cut(s) 55, 568, 583, 716, 860, 1086
MluNI TGGCCA 2 cut(s) 492, 747
MlyI GAGTC 2 cut(s) 281, 1021
MmeI TCCRAC 4 cut(s) 30, 240, 382, 1088
Mox20I TGGCCA 2 cut(s) 492, 747
Mph1103I ATGCAT 1 cut(s) 978
MscI TGGCCA 2 cut(s) 492, 747
MseI TTAA 1 cut(s) 863
Msp20I TGGCCA 2 cut(s) 492, 747
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 4 cut(s) 291, 421, 698, 872
MspR9I CCNGG 2 cut(s) 422, 967
Mva1269I GAATGC 1 cut(s) 843
MvaI CCWGG 1 cut(s) 967
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 3 cut(s) 464, 729, 1030
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 1 cut(s) 854
NdeII GATC 3 cut(s) 40, 346, 1060
NlaIII CATG 4 cut(s) 242, 692, 858, 980
NlaIV GGNNCC 2 cut(s) 426, 612
NmuCI GTSAC 2 cut(s) 890, 1003
NsiI ATGCAT 1 cut(s) 978
PceI AGGCCT 1 cut(s) 579
PctI GAATGC 1 cut(s) 843
PfeI GAWTC 1 cut(s) 1072
PinAI ACCGGT 1 cut(s) 871
PkrI GCNGC 2 cut(s) 317, 415
PleI GAGTC 2 cut(s) 281, 1020
PpsI GAGTC 2 cut(s) 281, 1020
PshBI ATTAAT 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 965
PspGI CCWGG 1 cut(s) 965
PspN4I GGNNCC 2 cut(s) 426, 612
PspPI GGNCC 1 cut(s) 630
RsaI GTAC 4 cut(s) 426, 622, 674, 764
RsaNI GTAC 4 cut(s) 425, 621, 673, 763
SaqAI TTAA 1 cut(s) 863
SatI GCNGC 2 cut(s) 316, 414
Sau3AI GATC 3 cut(s) 40, 346, 1060
Sau96I GGNCC 1 cut(s) 630
SchI GAGTC 2 cut(s) 281, 1021
ScrFI CCNGG 2 cut(s) 422, 967
SfaNI GCATC 1 cut(s) 405
SfcI CTRYAG 2 cut(s) 676, 787
SinI GGWCC 1 cut(s) 630
Sse9I AATT 6 cut(s) 55, 568, 583, 716, 860, 1086
SseBI AGGCCT 1 cut(s) 579
SsiI CCGC 6 cut(s) 222, 277, 316, 392, 477, 836
SspMI CTAG 4 cut(s) 32, 431, 645, 1099
StuI AGGCCT 1 cut(s) 579
StyD4I CCNGG 2 cut(s) 420, 965
StyI CCWWGG 1 cut(s) 854
TaaI ACNGT 2 cut(s) 505, 625
TaiI ACGT 1 cut(s) 487
TaqI TCGA 1 cut(s) 1084
TasI AATT 6 cut(s) 55, 568, 583, 716, 860, 1086
TatI WGTACW 2 cut(s) 672, 762
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 1072
Tru1I TTAA 1 cut(s) 863
Tru9I TTAA 1 cut(s) 863
TscAI CASTG 6 cut(s) 514, 630, 669, 899, 946, 1012
TseFI GTSAC 2 cut(s) 890, 1003
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 2 cut(s) 890, 1003
TspDTI ATGAA 3 cut(s) 12, 111, 705
TspRI CASTG 6 cut(s) 514, 630, 669, 899, 946, 1012
VpaK11BI GGWCC 1 cut(s) 630
VspI ATTAAT 1 cut(s) 863
XapI RAATTY 2 cut(s) 583, 716
XcmI CCANNNNNNNNNTGG 1 cut(s) 997
XspI CTAG 4 cut(s) 32, 431, 645, 1099
Zsp2I ATGCAT 1 cut(s) 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.