FvH4_5g28830

Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015388
Physical Location & Seq
Reverse (-)
19947931 .. 19953321
5391 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28830.t1

Sequence Viewer

Length: 1797 bp
ATGTTCTCCACCGTCGGATCTCTCTCACTCTTCACCTTCGCCTTCGCCTTCGCCTTCGCCTTCGCCTTCGCCTGCCTTCTCCTCTCCCCCGCCTCCGCCTCCGAGCCGGATCACAAGTATCAAGCAGAAGAAGCAGTTACCCTTTGGGTAAACAAAGTTGGCCCTTATAACAATCCACAAGAAACCTACAATTACTACAGTCTCCCGTTCTGTCGTCCATCTGATAATGCTGCTCACAAATGGGGTGGTCTTGGTGAAGTCTTGGGTGGAAATGAACTTATTGATAGCCAGATTGACATAAAGTTTGGAAAAAATGTGGATAGGGCTACCATTTGTCAGCTTGAACTTGATGATCATAAGGTTAAACAATTCAAGGATGCAATTGAGAATAGTTACTGGTTTGAGTTTTTCATGGATGATCTGCCTTTATGGGGCTTTGTTGGTGAGTTGCACCAGGATAAGAACAGTGAAAATGGCAAACATGTTCTTTTCACACACAAAAATATTATTATCAAGTATAATAAAGATCAGATAATTCATGTCAATCTCACTCAAGATAGTCCAAAGCCGCTGGAAGCGGGGAAGAAATTGGATCTGACGTACTCTGTCAAATGGATTCCAACCCCTATCACCTTTGCACGTCGCTTTGATGTATATTTGGACTACCCATTTTTCGAGCACCAAATCCATTGGTTCTCCATTTTCAATTCGTTCATGATGGTTATATTCCTTACTGGTTTGGTGTCAATGATATTGATGCGCACTCTTCGGAATGACTATGCAAAATATGCTCGGGAAGATGATGACCTAGAGACCCTGGAAAGAGATGTTAGTGAAGAGTCTGGCTGGAAACTTGTGCATGGGGATGTTTTTCGTCCTCCTCGGAGTTTGGTTATTCTTTCAGCTGTAGTTGGTACTGGAGCTCAGTTGGCGTTACTTGTTCTCCTTGTCATTTTACTGGCAATTGTTGGGATGTTGTATGTCGGGAGAGGTGCAATTGTTACAACTTTCATAGTGTGTTACGCTCTCACATCTTTCATTTCTGGTTATGTAAGTGGTGGGATGTATTCACGGCATGGGGGTAAAAACTGGATAAAGTCAATGATTCTCACAGCATCTTTATTTCCATTTATGTGCTTTGGAATTGGGTTCGTCTTGAATACAATTGCCATATTCTATGGGTCTCTAGCAGCTATTCCATTCGGCACTATGGTGGTTGTCTTTGTCATTTGGGGTTTCATCTCATTTCCTTTAGCGCTCCTTGGTACGGTTGTTGGAAGAAACTGGAGTGGTGCTCCTAATAATCCTTGTCGTGTCAAAACCATTCCTCGTCCAATTCCTGAGAAAAAATGGTACTTGACACCATCTGTGGTGTCGATGATGGGAGGGCTTTTACCCTTTGGAAGCATATTCATTGAAATGTACTTTGTCTTCACGTCCTTCTGGAATTACAAGGTGTACTATGTCTATGGCTTTATGCTGCTTGTGTTTCTGATTCTCATCATTGTTACTGTATGCGTGACGATTGTGGGGACCTACTTCTTGCTTAATGCTGAGAATTACCACTGGCAATGGACTTCTTTCTTTTCTGCTGCATCAACGGCTGTCTATGTGTACTTGTATTCAGTATATTACTACTATGCAAAGACCAAGATGTCAGGTTTCTTCCAGACAAGCTTCTATTTTGGATACACTTTGATGTTTTGCCTTGGCTTAGGGATCCTCTGTGGTGCTGTTGGATATTTGGGTTCAAATTTGTTTGTGAGGAGGATCTACAGAAACATCAAATGCGACTAG

Protein Analysis

599

Amino Acids

68.0

Weight (kDa)

7.91

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 62 - 555 2.5e-181 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10950
fragaria_vesca FvH4_5g28830
malus_domestica MD08G1236600.v1.1 MD15G1428600.v1.1
prunus_persica Prupe.1G573400_v2.0.a1 Prupe.1G573400_v2.0.a1
pyrus_communis pycom08g20490 pycom15g37960
rosa_chinensis RchiOBHm_Chr7g0221061
rosa_laevigata RLG00000002178
rosa_multiflora Rmu_sc0002105.1_g000001 Rmu_sc0012763.1_g000003
rosa_roxburghii Rroxscaffold_3G00238770
rosa_rugosa Rorug07G0200400
rosa_samantha Rh7AG339800
rosa_wichuraiana Rw7G028910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 168
AasI GACNNNNNNGTC 1 cut(s) 1654
Acc16I TGCGCA 1 cut(s) 761
AciI CCGC 4 cut(s) 90, 96, 569, 578
AclWI GGATC 6 cut(s) 25, 117, 600, 1714, 1727, 1778
AcsI RAATTY 1 cut(s) 1753
AfaI GTAC 7 cut(s) 602, 916, 1267, 1355, 1424, 1460, 1616
AfeI AGCGCT 1 cut(s) 1257
AfiI CCNNNNNNNGG 2 cut(s) 431, 1267
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 6 cut(s) 344, 373, 706, 1159, 1418, 1752
AjiI CACGTC 2 cut(s) 641, 1437
AjnI CCWGG 2 cut(s) 453, 816
AleI CACNNNNGTG 1 cut(s) 1211
AluBI AGCT 5 cut(s) 340, 905, 923, 1193, 1677
AluI AGCT 5 cut(s) 340, 905, 923, 1193, 1677
Alw21I GWGCWC 3 cut(s) 681, 925, 1297
Alw26I GTCTC 3 cut(s) 206, 806, 1188
AlwI GGATC 6 cut(s) 25, 117, 600, 1714, 1727, 1778
Ama87I CYCGRG 1 cut(s) 792
Aor51HI AGCGCT 1 cut(s) 1257
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 4 cut(s) 230, 1190, 1480, 1592
ApoI RAATTY 1 cut(s) 1753
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspLEI GCGC 2 cut(s) 762, 1258
AspS9I GGNCC 2 cut(s) 161, 1533
AsuHPI GGTGA 4 cut(s) 25, 266, 455, 622
AvaI CYCGRG 1 cut(s) 792
AvaII GGWCC 1 cut(s) 1533
BaeI ACNNNNGTAYC 2 cut(s) 1257, 1290
BamHI GGATCC 1 cut(s) 1719
BanII GRGCYC 1 cut(s) 925
BarI GAAGNNNNNNTAC 2 cut(s) 1415, 1447
BbsI GAAGAC 1 cut(s) 1423
Bbv12I GWGCWC 3 cut(s) 681, 925, 1297
BbvI GCAGC 4 cut(s) 217, 1202, 1467, 1579
BccI CCATC 4 cut(s) 226, 712, 1372, 1375
BceAI ACGGC 2 cut(s) 1088, 1617
BciT130I CCWGG 2 cut(s) 455, 818
BciVI GTATCC 1 cut(s) 1682
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 3 cut(s) 206, 806, 1188
BfaI CTAG 3 cut(s) 809, 1187, 1795
BfmI CTRYAG 3 cut(s) 196, 906, 1774
BfoI RGCGCY 1 cut(s) 1259
BfuI GTATCC 1 cut(s) 1682
BisI GCNGC 5 cut(s) 231, 569, 1191, 1481, 1593
BlsI GCNGC 5 cut(s) 232, 570, 1192, 1482, 1594
Bme1390I CCNGG 2 cut(s) 455, 818
Bme18I GGWCC 1 cut(s) 1533
BmeT110I CYCGRG 1 cut(s) 792
BmgBI CACGTC 2 cut(s) 641, 1437
BmgT120I GGNCC 2 cut(s) 161, 1533
BmiI GGNNCC 2 cut(s) 1534, 1721
BmrFI CCNGG 2 cut(s) 455, 818
BmsI GCATC 4 cut(s) 367, 747, 1124, 1604
BpiI GAAGAC 1 cut(s) 1423
BplI GAGNNNNNCTC 2 cut(s) 1279, 1311
BpmI CTGGAG 2 cut(s) 939, 1306
Bpu10I CCTNAGC 1 cut(s) 1714
BpuEI CTTGAG 1 cut(s) 537
BsaBI GATNNNNATC 1 cut(s) 1499
BsaI GGTCTC 2 cut(s) 806, 1188
BsaJI CCNNGG 4 cut(s) 816, 881, 1261, 1708
BsaXI ACNNNNNCTCC 2 cut(s) 927, 957
Bsc4I CCNNNNNNNGG 2 cut(s) 431, 1267
Bse1I ACTGG 7 cut(s) 401, 739, 922, 963, 1094, 1289, 1571
Bse3DI GCAATG 1 cut(s) 1577
Bse8I GATNNNNATC 1 cut(s) 1499
BseBI CCWGG 2 cut(s) 455, 818
BseDI CCNNGG 4 cut(s) 816, 881, 1261, 1708
BseGI GGATG 5 cut(s) 382, 421, 871, 978, 1068
BseJI GATNNNNATC 1 cut(s) 1499
BseLI CCNNNNNNNGG 2 cut(s) 431, 1267
BseMI GCAATG 1 cut(s) 1577
BseMII CTCAG 3 cut(s) 938, 1332, 1545
BseNI ACTGG 7 cut(s) 401, 739, 922, 963, 1094, 1289, 1571
BseRI GAGGAG 3 cut(s) 71, 870, 1780
BseXI GCAGC 4 cut(s) 217, 1202, 1467, 1579
BshFI GGCC 1 cut(s) 162
BsiHKAI GWGCWC 3 cut(s) 681, 925, 1297
BsiHKCI CYCGRG 1 cut(s) 792
BsiSI CCGG 1 cut(s) 107
BslFI GGGAC 1 cut(s) 1546
BslI CCNNNNNNNGG 2 cut(s) 431, 1267
BsmAI GTCTC 3 cut(s) 206, 806, 1188
BsmFI GGGAC 1 cut(s) 1546
BsnI GGCC 1 cut(s) 162
Bso31I GGTCTC 2 cut(s) 806, 1188
BsoBI CYCGRG 1 cut(s) 792
Bsp1286I GDGCHC 3 cut(s) 681, 925, 1297
Bsp143I GATC 8 cut(s) 17, 109, 352, 418, 526, 592, 1719, 1770
BspACI CCGC 4 cut(s) 90, 96, 569, 578
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 3 cut(s) 937, 1333, 1546
BspHI TCATGA 1 cut(s) 714
BspLI GGNNCC 2 cut(s) 1534, 1721
BspPI GGATC 6 cut(s) 25, 117, 600, 1714, 1727, 1778
BspTNI GGTCTC 2 cut(s) 806, 1188
BsrDI GCAATG 1 cut(s) 1577
BsrI ACTGG 7 cut(s) 401, 739, 922, 963, 1094, 1289, 1571
BssECI CCNNGG 4 cut(s) 816, 881, 1261, 1708
BssMI GATC 8 cut(s) 17, 109, 352, 418, 526, 592, 1719, 1770
BssT1I CCWWGG 2 cut(s) 1261, 1708
Bst2UI CCWGG 2 cut(s) 455, 818
Bst4CI ACNGT 5 cut(s) 13, 200, 467, 1270, 1513
Bst6I CTCTTC 3 cut(s) 35, 771, 831
BstAPI GCANNNNNTGC 1 cut(s) 788
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 4 cut(s) 924, 1341, 1554, 1714
BstF5I GGATG 5 cut(s) 382, 421, 871, 978, 1068
BstH2I RGCGCY 1 cut(s) 1259
BstHHI GCGC 2 cut(s) 762, 1258
BstKTI GATC 8 cut(s) 20, 112, 355, 421, 529, 595, 1722, 1773
BstMAI GTCTC 3 cut(s) 206, 806, 1188
BstMBI GATC 8 cut(s) 17, 109, 352, 418, 526, 592, 1719, 1770
BstMWI GCNNNNNNNGC 4 cut(s) 131, 788, 929, 1601
BstNI CCWGG 2 cut(s) 455, 818
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 453, 816
BstSFI CTRYAG 3 cut(s) 196, 906, 1774
BstV1I GCAGC 4 cut(s) 217, 1202, 1467, 1579
BstV2I GAAGAC 1 cut(s) 1423
BstX2I RGATCY 4 cut(s) 17, 592, 1719, 1770
BstYI RGATCY 4 cut(s) 17, 592, 1719, 1770
BsuI GTATCC 1 cut(s) 1682
BsuRI GGCC 1 cut(s) 162
BtrI CACGTC 2 cut(s) 641, 1437
BtsCI GGATG 5 cut(s) 382, 421, 871, 978, 1068
BtsIMutI CAGTG 2 cut(s) 472, 1564
Cac8I GCNNGC 1 cut(s) 73
CciI TCATGA 1 cut(s) 714
CfoI GCGC 2 cut(s) 762, 1258
Cfr13I GGNCC 2 cut(s) 161, 1533
Csp6I GTAC 7 cut(s) 601, 915, 1266, 1354, 1423, 1459, 1615
CspCI CAANNNNNGTGG 2 cut(s) 226, 261
CviAII CATG 6 cut(s) 412, 482, 539, 715, 860, 1076
CviQI GTAC 7 cut(s) 601, 915, 1266, 1354, 1423, 1459, 1615
DdeI CTNAG 4 cut(s) 924, 1341, 1554, 1714
DpnI GATC 8 cut(s) 19, 111, 354, 420, 528, 594, 1721, 1772
DpnII GATC 8 cut(s) 17, 109, 352, 418, 526, 592, 1719, 1770
DrdI GACNNNNNNGTC 1 cut(s) 1654
DseDI GACNNNNNNGTC 1 cut(s) 1654
Eam1104I CTCTTC 3 cut(s) 35, 771, 831
EarI CTCTTC 3 cut(s) 35, 771, 831
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 923
Eco130I CCWWGG 2 cut(s) 1261, 1708
Eco24I GRGCYC 1 cut(s) 925
Eco31I GGTCTC 2 cut(s) 806, 1188
Eco47I GGWCC 1 cut(s) 1533
Eco47III AGCGCT 1 cut(s) 1257
Eco53kI GAGCTC 1 cut(s) 923
Eco88I CYCGRG 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 923
EcoO109I RGGNCCY 1 cut(s) 1533
EcoRII CCWGG 2 cut(s) 453, 816
EcoT14I CCWWGG 2 cut(s) 1261, 1708
EcoT38I GRGCYC 1 cut(s) 925
ErhI CCWWGG 2 cut(s) 1261, 1708
FaeI CATG 6 cut(s) 415, 485, 542, 718, 863, 1079
FaqI GGGAC 1 cut(s) 1546
FatI CATG 6 cut(s) 411, 481, 538, 714, 859, 1075
FauI CCCGC 2 cut(s) 97, 571
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 5 cut(s) 231, 569, 1191, 1481, 1593
FokI GGATG 5 cut(s) 389, 428, 878, 985, 1075
FriOI GRGCYC 1 cut(s) 925
Fsp4HI GCNGC 5 cut(s) 231, 569, 1191, 1481, 1593
FspAI RTGCGCAY 1 cut(s) 761
FspBI CTAG 3 cut(s) 809, 1187, 1795
FspI TGCGCA 1 cut(s) 761
GlaI GCGC 2 cut(s) 761, 1257
GluI GCNGC 5 cut(s) 231, 569, 1191, 1481, 1593
GsuI CTGGAG 2 cut(s) 939, 1306
HaeII RGCGCY 1 cut(s) 1259
HaeIII GGCC 1 cut(s) 162
HapII CCGG 1 cut(s) 107
HhaI GCGC 2 cut(s) 762, 1258
Hin1II CATG 6 cut(s) 415, 485, 542, 718, 863, 1079
Hin6I GCGC 2 cut(s) 760, 1256
HinP1I GCGC 2 cut(s) 760, 1256
HindIII AAGCTT 1 cut(s) 1675
HinfI GANTC 4 cut(s) 616, 839, 1105, 1495
HpaII CCGG 1 cut(s) 107
HphI GGTGA 4 cut(s) 25, 266, 455, 622
Hpy166II GTNNAC 3 cut(s) 151, 1459, 1615
Hpy188I TCNGA 8 cut(s) 17, 103, 223, 531, 597, 771, 885, 1494
Hpy188III TCNNGA 8 cut(s) 554, 715, 794, 985, 1156, 1340, 1444, 1669
Hpy8I GTNNAC 3 cut(s) 151, 1459, 1615
Hpy99I CGWCG 2 cut(s) 17, 645
HpyAV CCTTC 8 cut(s) 46, 52, 58, 64, 70, 76, 86, 1450
HpyCH4III ACNGT 5 cut(s) 13, 200, 467, 1270, 1513
HpyCH4IV ACGT 3 cut(s) 599, 640, 1436
HpyCH4V TGCA 8 cut(s) 380, 451, 638, 782, 859, 995, 1595, 1643
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 788, 929, 1601
HpyF3I CTNAG 4 cut(s) 924, 1341, 1554, 1714
HpySE526I ACGT 3 cut(s) 599, 640, 1436
Hsp92II CATG 6 cut(s) 415, 485, 542, 718, 863, 1079
HspAI GCGC 2 cut(s) 760, 1256
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 8 cut(s) 17, 109, 352, 418, 526, 592, 1719, 1770
LmnI GCTCC 3 cut(s) 920, 1263, 1300
Lsp1109I GCAGC 4 cut(s) 217, 1202, 1467, 1579
LweI GCATC 4 cut(s) 367, 747, 1124, 1604
MaeI CTAG 3 cut(s) 809, 1187, 1795
MaeII ACGT 3 cut(s) 599, 640, 1436
MaeIII GTNAC 7 cut(s) 136, 392, 933, 1000, 1019, 1507, 1519
MalI GATC 8 cut(s) 19, 111, 354, 420, 528, 594, 1721, 1772
MboI GATC 8 cut(s) 17, 109, 352, 418, 526, 592, 1719, 1770
MboII GAAGA 9 cut(s) 22, 140, 595, 758, 809, 848, 1290, 1423, 1657
MfeI CAATTG 4 cut(s) 381, 963, 996, 1164
MflI RGATCY 4 cut(s) 17, 592, 1719, 1770
MhlI GDGCHC 3 cut(s) 681, 925, 1297
MlyI GAGTC 1 cut(s) 848
MmeI TCCRAC 3 cut(s) 644, 1255, 1717
MseI TTAA 2 cut(s) 363, 1548
MslI CAYNNNNRTG 5 cut(s) 864, 1132, 1211, 1418, 1697
MspA1I CMGCKG 2 cut(s) 571, 905
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 2 cut(s) 455, 818
MunI CAATTG 4 cut(s) 381, 963, 996, 1164
MvaI CCWGG 2 cut(s) 455, 818
MwoI GCNNNNNNNGC 4 cut(s) 131, 788, 929, 1601
NdeII GATC 8 cut(s) 17, 109, 352, 418, 526, 592, 1719, 1770
NlaIII CATG 6 cut(s) 415, 485, 542, 718, 863, 1079
NlaIV GGNNCC 2 cut(s) 1534, 1721
NmuCI GTSAC 1 cut(s) 1519
NsbI TGCGCA 1 cut(s) 761
NspI RCATGY 1 cut(s) 485
OliI CACNNNNGTG 1 cut(s) 1211
PagI TCATGA 1 cut(s) 714
PciI ACATGT 1 cut(s) 481
PfeI GAWTC 3 cut(s) 616, 1105, 1495
PkrI GCNGC 5 cut(s) 232, 570, 1192, 1482, 1594
PleI GAGTC 1 cut(s) 847
PpsI GAGTC 1 cut(s) 847
PpuMI RGGWCCY 1 cut(s) 1533
PscI ACATGT 1 cut(s) 481
PsiI TTATAA 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 925
Psp5II RGGWCCY 1 cut(s) 1533
Psp6I CCWGG 2 cut(s) 453, 816
PspGI CCWGG 2 cut(s) 453, 816
PspN4I GGNNCC 2 cut(s) 1534, 1721
PspPI GGNCC 2 cut(s) 161, 1533
PspPPI RGGWCCY 1 cut(s) 1533
PsuI RGATCY 4 cut(s) 17, 592, 1719, 1770
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 7 cut(s) 602, 916, 1267, 1355, 1424, 1460, 1616
RsaNI GTAC 7 cut(s) 601, 915, 1266, 1354, 1423, 1459, 1615
RseI CAYNNNNRTG 5 cut(s) 864, 1132, 1211, 1418, 1697
SacI GAGCTC 1 cut(s) 925
SaqAI TTAA 2 cut(s) 363, 1548
SatI GCNGC 5 cut(s) 231, 569, 1191, 1481, 1593
Sau3AI GATC 8 cut(s) 17, 109, 352, 418, 526, 592, 1719, 1770
Sau96I GGNCC 2 cut(s) 161, 1533
SchI GAGTC 1 cut(s) 848
ScrFI CCNGG 2 cut(s) 455, 818
SduI GDGCHC 3 cut(s) 681, 925, 1297
SfaNI GCATC 4 cut(s) 367, 747, 1124, 1604
SfcI CTRYAG 3 cut(s) 196, 906, 1774
SinI GGWCC 1 cut(s) 1533
SmiMI CAYNNNNRTG 5 cut(s) 864, 1132, 1211, 1418, 1697
SmlI CTYRAG 1 cut(s) 552
SmoI CTYRAG 1 cut(s) 552
SsiI CCGC 4 cut(s) 90, 96, 569, 578
SspI AATATT 1 cut(s) 505
SspMI CTAG 3 cut(s) 809, 1187, 1795
SstI GAGCTC 1 cut(s) 925
StyD4I CCNGG 2 cut(s) 453, 816
StyI CCWWGG 2 cut(s) 1261, 1708
TaaI ACNGT 5 cut(s) 13, 200, 467, 1270, 1513
TaiI ACGT 3 cut(s) 602, 643, 1439
TaqI TCGA 2 cut(s) 675, 1376
TatI WGTACW 3 cut(s) 1422, 1458, 1614
TauI GCSGC 1 cut(s) 571
TfiI GAWTC 3 cut(s) 616, 1105, 1495
Tru1I TTAA 2 cut(s) 363, 1548
Tru9I TTAA 2 cut(s) 363, 1548
TscAI CASTG 2 cut(s) 472, 1571
TseFI GTSAC 1 cut(s) 1519
TseI GCWGC 4 cut(s) 230, 1190, 1480, 1592
Tsp45I GTSAC 1 cut(s) 1519
TspDTI ATGAA 8 cut(s) 288, 400, 527, 703, 1000, 1027, 1228, 1402
TspRI CASTG 2 cut(s) 472, 1571
VpaK11BI GGWCC 1 cut(s) 1533
XapI RAATTY 1 cut(s) 1753
XceI RCATGY 1 cut(s) 485
XspI CTAG 3 cut(s) 809, 1187, 1795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.