MD15G1428600.v1.1

Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015388
Physical Location & Seq
Forward (+)
52927570 .. 52933916
6347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1428600.v1.1.491

Sequence Viewer

Length: 1941 bp
ATGCGTTCCGAAGTCTATCTTTTGAGAAGACTTTGGAGAGTAATTGAGTCGAGTCCGAGTGGCATCAGCCTCAGCCTCTGCCTCCTCTGCCTCTGGGAGAGATCACAACACAAAACATTACACCACCTTCTCACGCTCACCGTCCAGATCCCACCCTCGCCGGCGATCGCCATGTTCTCCTCCGTCCGATCTCTCTCACCCCTCCTCCTCTTCGTCTTCTTCCTCCTCGCTCCCGCCTTCGCCTCCGAGTCAGATCACAAGTATAAAACAGAAGAATCAGTTACCCTTTGGGTGAATAAAGTTGGGCCCTACAATAATCCACAAGAAACATACAATTACTACAGTCTTCCTTTCTGTCATCAATCTGAAAATGCTGCTCACAAATGGGGTGGTCTTGGTGAAGTCCTGGGTGGAAATGAGCTGATTGATAGCCAGATTGAGATAAAGTTTCAAAAAAATGTGGACAGGGCTACCATTTGCCAACTTGAACTTGACGATCAAAAGGTTAAACACTTCAAGGATGCAATCGAGAATAACTACTGGTTTGAATTTTTCATGGATGATCTGCCTTTATGGGGCTTTGTTGGTGAGTTGCACCCGGACAAGAACAGTGAAAATGGCAAGCATGTCCTTTACACGCATAAAAATATTATTGTTAAATACAATAAAGATCAGATCATTCATGTGAATCTCACTCAAGATAGTCCAAAGCCGTTGGAAGCAGGGAAGAAATTGGACCTAACTTACTCTGTCAAATGGATTGCAACCACTATCACATTTGCACGTCGATTTGATGTCTATTTGGACTACCCTTTTTTTGAGCACCAAATACATTGGTTCTCGATTTTCAATTCATTCATGATGGTTATTTTCCTTACTGGTTTGGTGTCAATGATATTGATGCGGACTCTTAGGAATGACTATGCAAAGTATGCTCGGGAAGACGACGATCTGGAGACTCTGGAAAGAGATGTCAGTGAAGAGTCTGGGTGGAAACTTGTGCATGGGGATGTTTTTCGGCCTTCTCGAAATTTGGTTATTCTTTCAGCCGTTGTTGGCACGGGCGCTCAGCTAGCGTTGCTTGTTCTTCTCGTCATCTTACTGGCAATTGTTGGGATGTTGTATGTTGGGAGAGGTGCAATTGTTACAACTTTCATAGTGTGTTACGCCCTTACATCTTTCATTTCTGGTTATGTAAGTGGTGGGATGTATTCAAGACATGGTGGTAAAAATTGGATAAAGTCAATGATACTCACAGCATCTCTATTTCCATTTATGTGCTTTGGGATTGGGTTCATCTTGAACACAATTGCTATATTTTACGGGTCACTGGCTGCTATTCCTTTTGGTACAATGGTGGTTGTTTTTGTGATTTGGGCATTCATCTCTTTTCCTCTAGCACTTCTTGGTACAGTTGTTGGAAGAAACTGGAGTGGTGCTCCTAACAATCCTTGTCGTGTGAAAACCATTCCTCGTCCTATTCCTGAGAAGAAATGGTATTTAACCCCATCTGTAGTCTCATTGATGGGAGGACTTCTGCCCTTTGGAAGCATATTTATCGAAATGTATTTTGTCTTCACATCCTTCTGGAATTACAAGGTGTATTATGTCTATGGCTTTATGCTGCTTGTTTTTCTGATTCTCATCATTGTTACTGTGTGCGTGACAATTGTGGGGACATACTTCTTGCTTAATGCTGAGAACTACCACTGGCAATGGACTTCTTTCTTTTCTGCTGCATCAACAGCTGTCTACGTGTACTTGTATTCTATATATTACTACTATGCAAAGACCAAGATGTCGGGTTTCTTCCAGACCAGCTTCTATTTTGGATACACTTTGATGTTTTGCCTTGGCTTAGGAATCCTTTGCGGAGCTGTTGGATATCTGGGTTCTAATTTGTTTGTAAGGAGGATCTACAGAAACATCAAGTGCGACTAG

Protein Analysis

647

Amino Acids

73.91

Weight (kDa)

8.54

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 110 - 603 6.4e-180 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10950
fragaria_vesca FvH4_5g28830
malus_domestica MD08G1236600.v1.1 MD15G1428600.v1.1
prunus_persica Prupe.1G573400_v2.0.a1 Prupe.1G573400_v2.0.a1
pyrus_communis pycom08g20490 pycom15g37960
rosa_chinensis RchiOBHm_Chr7g0221061
rosa_laevigata RLG00000002178
rosa_multiflora Rmu_sc0002105.1_g000001 Rmu_sc0012763.1_g000003
rosa_roxburghii Rroxscaffold_3G00238770
rosa_rugosa Rorug07G0200400
rosa_samantha Rh7AG339800
rosa_wichuraiana Rw7G028910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1798
AccI GTMKAC 1 cut(s) 1752
AciI CCGC 3 cut(s) 234, 904, 1872
AclWI GGATC 2 cut(s) 142, 1922
AcsI RAATTY 2 cut(s) 548, 1030
AfaI GTAC 3 cut(s) 1351, 1411, 1760
AfiI CCNNNNNNNGG 1 cut(s) 575
AflIII ACRYGT 1 cut(s) 1755
AgsI TTSAA 7 cut(s) 452, 488, 517, 548, 850, 1215, 1303
AjiI CACGTC 1 cut(s) 785
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 5 cut(s) 421, 1072, 1748, 1821, 1877
AluI AGCT 5 cut(s) 421, 1072, 1748, 1821, 1877
Alw21I GWGCWC 2 cut(s) 825, 1441
Alw26I GTCTC 2 cut(s) 950, 1522
AlwI GGATC 2 cut(s) 142, 1922
AlwNI CAGNNNCTG 1 cut(s) 78
Ama87I CYCGRG 1 cut(s) 936
AoxI GGCC 2 cut(s) 305, 1019
ApaI GGGCCC 1 cut(s) 309
ApeKI GCWGC 4 cut(s) 374, 1334, 1624, 1736
ApoI RAATTY 2 cut(s) 548, 1030
AsiSI GCGATCGC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 1067
AspS9I GGNCC 3 cut(s) 305, 306, 736
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 5 cut(s) 130, 189, 304, 410, 599
AsuNHI GCTAGC 1 cut(s) 1072
AvaI CYCGRG 1 cut(s) 936
AvaII GGWCC 1 cut(s) 736
BaeGI GKGCMC 1 cut(s) 309
BaeI ACNNNNGTAYC 4 cut(s) 1341, 1374, 1401, 1434
BanII GRGCYC 1 cut(s) 309
BarI GAAGNNNNNNTAC 2 cut(s) 1559, 1591
BbsI GAAGAC 5 cut(s) 34, 208, 338, 948, 1567
Bbv12I GWGCWC 2 cut(s) 825, 1441
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 4 cut(s) 361, 1321, 1611, 1723
BccI CCATC 3 cut(s) 856, 1516, 1519
BceAI ACGGC 2 cut(s) 697, 1034
BcgI CGANNNNNNTGC 2 cut(s) 1540, 1574
BciT130I CCWGG 1 cut(s) 407
BciVI GTATCC 1 cut(s) 1826
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 2 cut(s) 950, 1522
BfaI CTAG 3 cut(s) 1073, 1397, 1939
BfmI CTRYAG 3 cut(s) 340, 1512, 1918
BfoI RGCGCY 1 cut(s) 1068
BfuI GTATCC 1 cut(s) 1826
BisI GCNGC 4 cut(s) 375, 1335, 1625, 1737
BlpI GCTNAGC 1 cut(s) 1068
BlsI GCNGC 4 cut(s) 376, 1336, 1626, 1738
Bme1390I CCNGG 2 cut(s) 407, 599
Bme18I GGWCC 1 cut(s) 736
BmeT110I CYCGRG 1 cut(s) 936
BmgBI CACGTC 1 cut(s) 785
BmgT120I GGNCC 3 cut(s) 305, 306, 736
BmiI GGNNCC 1 cut(s) 307
BmrFI CCNGG 2 cut(s) 407, 599
BmsI GCATC 5 cut(s) 72, 511, 891, 1268, 1748
BmtI GCTAGC 1 cut(s) 1076
BpiI GAAGAC 5 cut(s) 34, 208, 338, 948, 1567
BplI GAGNNNNNCTC 2 cut(s) 1423, 1455
BpmI CTGGAG 2 cut(s) 974, 1450
Bpu10I CCTNAGC 2 cut(s) 71, 1858
Bpu1102I GCTNAGC 1 cut(s) 1068
BpuEI CTTGAG 1 cut(s) 681
BpuMI CCSGG 1 cut(s) 599
BsaAI YACGTR 1 cut(s) 1756
BsaBI GATNNNNATC 1 cut(s) 1643
BsaJI CCNNGG 2 cut(s) 406, 1852
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 6 cut(s) 545, 883, 1107, 1335, 1433, 1715
Bse3DI GCAATG 1 cut(s) 1721
Bse8I GATNNNNATC 1 cut(s) 1643
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 2 cut(s) 406, 1852
BseGI GGATG 6 cut(s) 526, 565, 1015, 1122, 1212, 1580
BseJI GATNNNNATC 1 cut(s) 1643
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMI GCAATG 1 cut(s) 1721
BseMII CTCAG 4 cut(s) 85, 1082, 1476, 1689
BseNI ACTGG 6 cut(s) 545, 883, 1107, 1335, 1433, 1715
BseRI GAGGAG 5 cut(s) 74, 169, 194, 197, 215
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 4 cut(s) 361, 1321, 1611, 1723
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 307, 1021
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 2 cut(s) 825, 1441
BsiHKCI CYCGRG 1 cut(s) 936
BsiSI CCGG 2 cut(s) 161, 599
BslFI GGGAC 1 cut(s) 1690
BslI CCNNNNNNNGG 1 cut(s) 575
BsmAI GTCTC 2 cut(s) 950, 1522
BsmFI GGGAC 1 cut(s) 1690
BsmI GAATGC 1 cut(s) 1379
BsnI GGCC 2 cut(s) 307, 1021
BsoBI CYCGRG 1 cut(s) 936
Bsp120I GGGCCC 1 cut(s) 305
Bsp1286I GDGCHC 3 cut(s) 309, 825, 1441
Bsp1720I GCTNAGC 1 cut(s) 1068
BspACI CCGC 3 cut(s) 234, 904, 1872
BspANI GGCC 2 cut(s) 307, 1021
BspCNI CTCAG 4 cut(s) 84, 1081, 1477, 1690
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 1 cut(s) 307
BspOI GCTAGC 1 cut(s) 1076
BspPI GGATC 2 cut(s) 142, 1922
BsrDI GCAATG 1 cut(s) 1721
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 6 cut(s) 545, 883, 1107, 1335, 1433, 1715
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 406, 1852
BssT1I CCWWGG 1 cut(s) 1852
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 5 cut(s) 142, 344, 611, 1414, 1657
Bst6I CTCTTC 2 cut(s) 215, 975
BstAPI GCANNNNNTGC 1 cut(s) 932
BstBAI YACGTR 1 cut(s) 1756
BstC8I GCNNGC 3 cut(s) 162, 623, 1074
BstDEI CTNAG 6 cut(s) 71, 911, 1068, 1485, 1698, 1858
BstF5I GGATG 6 cut(s) 526, 565, 1015, 1122, 1212, 1580
BstH2I RGCGCY 1 cut(s) 1068
BstHHI GCGC 1 cut(s) 1067
BstMAI GTCTC 2 cut(s) 950, 1522
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 5 cut(s) 87, 932, 1073, 1078, 1745
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 629
BstSCI CCNGG 2 cut(s) 405, 597
BstSFI CTRYAG 3 cut(s) 340, 1512, 1918
BstSLI GKGCMC 1 cut(s) 309
BstV1I GCAGC 4 cut(s) 361, 1321, 1611, 1723
BstV2I GAAGAC 5 cut(s) 34, 208, 338, 948, 1567
BstX2I RGATCY 2 cut(s) 147, 1914
BstYI RGATCY 2 cut(s) 147, 1914
BsuI GTATCC 1 cut(s) 1826
BsuRI GGCC 2 cut(s) 307, 1021
BtrI CACGTC 1 cut(s) 785
BtsCI GGATG 6 cut(s) 526, 565, 1015, 1122, 1212, 1580
BtsIMutI CAGTG 4 cut(s) 616, 982, 1328, 1708
Cac8I GCNNGC 3 cut(s) 162, 623, 1074
CaiI CAGNNNCTG 1 cut(s) 78
CciI TCATGA 1 cut(s) 858
CfoI GCGC 1 cut(s) 1067
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 3 cut(s) 305, 306, 736
Csp6I GTAC 3 cut(s) 1350, 1410, 1759
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 7 cut(s) 172, 556, 626, 683, 859, 1004, 1220
CviQI GTAC 3 cut(s) 1350, 1410, 1759
DdeI CTNAG 6 cut(s) 71, 911, 1068, 1485, 1698, 1858
DrdI GACNNNNNNGTC 1 cut(s) 1798
DseDI GACNNNNNNGTC 1 cut(s) 1798
Eam1104I CTCTTC 2 cut(s) 215, 975
EarI CTCTTC 2 cut(s) 215, 975
Eco130I CCWWGG 1 cut(s) 1852
Eco24I GRGCYC 1 cut(s) 309
Eco32I GATATC 1 cut(s) 1886
Eco47I GGWCC 1 cut(s) 736
Eco88I CYCGRG 1 cut(s) 936
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 405
EcoRV GATATC 1 cut(s) 1886
EcoT14I CCWWGG 1 cut(s) 1852
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 1852
FaeI CATG 7 cut(s) 175, 559, 629, 686, 862, 1007, 1223
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 1 cut(s) 1690
FatI CATG 7 cut(s) 171, 555, 625, 682, 858, 1003, 1219
FauI CCCGC 1 cut(s) 241
FblI GTMKAC 1 cut(s) 1752
Fnu4HI GCNGC 4 cut(s) 375, 1335, 1625, 1737
FokI GGATG 6 cut(s) 533, 572, 1022, 1129, 1219, 1567
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 4 cut(s) 375, 1335, 1625, 1737
FspBI CTAG 3 cut(s) 1073, 1397, 1939
GlaI GCGC 1 cut(s) 1066
GluI GCNGC 4 cut(s) 375, 1335, 1625, 1737
GsuI CTGGAG 2 cut(s) 974, 1450
HaeII RGCGCY 1 cut(s) 1068
HaeIII GGCC 2 cut(s) 307, 1021
HapII CCGG 2 cut(s) 161, 599
HhaI GCGC 1 cut(s) 1067
Hin1II CATG 7 cut(s) 175, 559, 629, 686, 862, 1007, 1223
Hin6I GCGC 1 cut(s) 1065
HinP1I GCGC 1 cut(s) 1065
HpaII CCGG 2 cut(s) 161, 599
HphI GGTGA 5 cut(s) 130, 189, 304, 410, 599
Hpy166II GTNNAC 3 cut(s) 463, 1753, 1759
Hpy188I TCNGA 8 cut(s) 10, 57, 188, 247, 253, 367, 675, 1638
Hpy8I GTNNAC 3 cut(s) 463, 1753, 1759
Hpy99I CGWCG 2 cut(s) 789, 950
HpyAV CCTTC 4 cut(s) 137, 247, 1032, 1594
HpyCH4III ACNGT 5 cut(s) 142, 344, 611, 1414, 1657
HpyCH4IV ACGT 2 cut(s) 784, 1755
HpyCH4V TGCA 9 cut(s) 524, 595, 764, 782, 926, 1003, 1139, 1739, 1787
HpyF10VI GCNNNNNNNGC 5 cut(s) 87, 932, 1073, 1078, 1745
HpyF3I CTNAG 6 cut(s) 71, 911, 1068, 1485, 1698, 1858
HpySE526I ACGT 2 cut(s) 784, 1755
Hsp92II CATG 7 cut(s) 175, 559, 629, 686, 862, 1007, 1223
HspAI GCGC 1 cut(s) 1065
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
LmnI GCTCC 3 cut(s) 235, 1444, 1874
Lsp1109I GCAGC 4 cut(s) 361, 1321, 1611, 1723
LweI GCATC 5 cut(s) 72, 511, 891, 1268, 1748
MaeI CTAG 3 cut(s) 1073, 1397, 1939
MaeII ACGT 2 cut(s) 784, 1755
MaeIII GTNAC 6 cut(s) 280, 1144, 1163, 1326, 1651, 1663
MfeI CAATTG 4 cut(s) 1107, 1140, 1308, 1668
MflI RGATCY 2 cut(s) 147, 1914
MhlI GDGCHC 3 cut(s) 309, 825, 1441
MlyI GAGTC 6 cut(s) 56, 61, 257, 901, 952, 992
MmeI TCCRAC 3 cut(s) 696, 1399, 1861
MreI CGCCGGCG 1 cut(s) 160
MroNI GCCGGC 1 cut(s) 160
MseI TTAA 4 cut(s) 507, 657, 1502, 1692
MslI CAYNNNNRTG 4 cut(s) 683, 1008, 1276, 1841
MspA1I CMGCKG 1 cut(s) 1748
MspI CCGG 2 cut(s) 161, 599
MspR9I CCNGG 2 cut(s) 407, 599
MunI CAATTG 4 cut(s) 1107, 1140, 1308, 1668
Mva1269I GAATGC 1 cut(s) 1379
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 5 cut(s) 87, 932, 1073, 1078, 1745
NaeI GCCGGC 1 cut(s) 162
NciI CCSGG 1 cut(s) 599
NgoMIV GCCGGC 1 cut(s) 160
NheI GCTAGC 1 cut(s) 1072
NlaIII CATG 7 cut(s) 175, 559, 629, 686, 862, 1007, 1223
NlaIV GGNNCC 1 cut(s) 307
NmuCI GTSAC 2 cut(s) 1326, 1663
NspI RCATGY 1 cut(s) 629
PagI TCATGA 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 1024
PctI GAATGC 1 cut(s) 1379
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 4 cut(s) 275, 688, 1639, 1863
PkrI GCNGC 4 cut(s) 376, 1336, 1626, 1738
Ple19I CGATCG 1 cut(s) 168
PleI GAGTC 6 cut(s) 55, 60, 256, 901, 952, 991
PpsI GAGTC 6 cut(s) 55, 60, 256, 901, 952, 991
Ppu21I YACGTR 1 cut(s) 1756
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 1 cut(s) 307
PspOMI GGGCCC 1 cut(s) 305
PspPI GGNCC 3 cut(s) 305, 306, 736
PstNI CAGNNNCTG 1 cut(s) 78
PsuI RGATCY 2 cut(s) 147, 1914
PvuI CGATCG 1 cut(s) 168
PvuII CAGCTG 1 cut(s) 1748
RgaI GCGATCGC 1 cut(s) 168
RsaI GTAC 3 cut(s) 1351, 1411, 1760
RsaNI GTAC 3 cut(s) 1350, 1410, 1759
RseI CAYNNNNRTG 4 cut(s) 683, 1008, 1276, 1841
SaqAI TTAA 4 cut(s) 507, 657, 1502, 1692
SatI GCNGC 4 cut(s) 375, 1335, 1625, 1737
Sau96I GGNCC 3 cut(s) 305, 306, 736
SchI GAGTC 6 cut(s) 56, 61, 257, 901, 952, 992
ScrFI CCNGG 2 cut(s) 407, 599
SduI GDGCHC 3 cut(s) 309, 825, 1441
SfaAI GCGATCGC 1 cut(s) 168
SfaNI GCATC 5 cut(s) 72, 511, 891, 1268, 1748
SfcI CTRYAG 3 cut(s) 340, 1512, 1918
SgfI GCGATCGC 1 cut(s) 168
SgrAI CRCCGGYG 1 cut(s) 160
SinI GGWCC 1 cut(s) 736
SmiMI CAYNNNNRTG 4 cut(s) 683, 1008, 1276, 1841
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
SsiI CCGC 3 cut(s) 234, 904, 1872
SspI AATATT 1 cut(s) 649
SspMI CTAG 3 cut(s) 1073, 1397, 1939
StyD4I CCNGG 2 cut(s) 405, 597
StyI CCWWGG 1 cut(s) 1852
TaaI ACNGT 5 cut(s) 142, 344, 611, 1414, 1657
TaiI ACGT 2 cut(s) 787, 1758
TaqI TCGA 6 cut(s) 50, 528, 787, 842, 1027, 1560
TatI WGTACW 1 cut(s) 1758
TfiI GAWTC 4 cut(s) 275, 688, 1639, 1863
Tru1I TTAA 4 cut(s) 507, 657, 1502, 1692
Tru9I TTAA 4 cut(s) 507, 657, 1502, 1692
TscAI CASTG 4 cut(s) 616, 982, 1335, 1715
TseFI GTSAC 2 cut(s) 1326, 1663
TseI GCWGC 4 cut(s) 374, 1334, 1624, 1736
Tsp45I GTSAC 2 cut(s) 1326, 1663
TspDTI ATGAA 8 cut(s) 544, 671, 843, 847, 1144, 1171, 1285, 1372
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 4 cut(s) 616, 982, 1335, 1715
VpaK11BI GGWCC 1 cut(s) 736
XapI RAATTY 2 cut(s) 548, 1030
XceI RCATGY 1 cut(s) 629
XmiI GTMKAC 1 cut(s) 1752
XspI CTAG 3 cut(s) 1073, 1397, 1939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.