Rorug07G0200400

Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0015388
Physical Location & Seq
Reverse (-)
17385668 .. 17386780
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0200400.1

Sequence Viewer

Length: 1113 bp
ATGCATGCACTTTTACCAGCTGATAATCTCCTCAAGCCCTACATTCCCCTGTCGTCCAGGGTTGGGGCGACCCGGCTCACCGTCCAATGCTCCGACTCTCTCAACTACCACAACTCCGTCTCCGACTGGCAGAGCTCCTGCGCCATTCTCGCCAGCAAGGTCGTCTCCTCCAAGCCTGAACAATCTGCTGGAACCATCGCCGACCTCTCGCCGAAGCCTATGCACGGCGCACAGCTGCGCGTGGCTTACCAGGGAGTTCCCGGCGCTTACTCGGAGGCCGCGGCCGGTAAAGCCTACCCAAACTGCGAGGCCATTCCCTGCGATCAATTCGAAGATGCTTTCCAGGCCGTGGAGCTCTGGATCGCCGACCGGGCCGTTTTGCCAATTGAAAACTCCCTCGGCGGCTCCATCCACCGGAATTACGATCTTCTCCTCCGCCACAGGCTCCACATTGTCGGCGAGGTCCAGTTACTCGTCCACCACTGCCTCTTAGCACTCCCGGGGGTTCAAAAAGACTGCTTGACCCGAGTCATCTCCCACTCTCAGGCCCTGGCCCAGTGCGAGCTCACTCTCACCAAGCTCGGCCTCAACGTCGAGCGCGAAGCCGTTGACAACACCGCCGGGGCCGCCGAGTTCATCGCCTCCAACAACCTTCGGGACACCGCCGCAATCGCCAGCGCACGAGCCGCCGATATCTACGGCCTCAACATCCTCGCTGACGGTATTCAGGACGACTCGAGCAACGTGACCCGGTTTGTGATGCTTGCCCGGGAGCCCATAGTTCCCCGGACGGACCGGCCCTTCAAGACCAGTATCGTCTTCGCTCACGACAAAGGGACGTCGGTGCTGTTCAAGGTGCTATCGGCGTTCGCCTTCAGGAACATCAGCTTGACCAAGATCGAGTCGCGGCCAAACCGCAACCGTCCGATCAGGCTGGTGAACGACTCGAACGAAGGGCGCTTCGAGTACTTGTTCTACGTCGACTTCGAGGCGTCAATGGCCGAGGTTAGGGCTCAGAATGCTTTGGAGGAGGTCCAGGAGTTCACGTCTTTCTTGAGGGTCCTTGGTAGCTACCCCAGGGATATGATCCCCTTGGTGTCCTTCGCGGGGTGA

Protein Analysis

370

Amino Acids

40.58

Weight (kDa)

6.07

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDT PF00800 81 - 258 1e-57 Prephenate dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10950
fragaria_vesca FvH4_5g28830
malus_domestica MD08G1236600.v1.1 MD15G1428600.v1.1
prunus_persica Prupe.1G573400_v2.0.a1 Prupe.1G573400_v2.0.a1
pyrus_communis pycom08g20490 pycom15g37960
rosa_chinensis RchiOBHm_Chr7g0221061
rosa_laevigata RLG00000002178
rosa_multiflora Rmu_sc0002105.1_g000001 Rmu_sc0012763.1_g000003
rosa_roxburghii Rroxscaffold_3G00238770
rosa_rugosa Rorug07G0200400
rosa_samantha Rh7AG339800
rosa_wichuraiana Rw7G028910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 842
AccB7I CCANNNNNTGG 1 cut(s) 349
AccI GTMKAC 1 cut(s) 981
AccII CGCG 5 cut(s) 240, 281, 600, 907, 1106
AclWI GGATC 2 cut(s) 368, 1081
AcoI YGGCCR 3 cut(s) 282, 908, 999
AcuI CTGAAG 1 cut(s) 859
AcyI GRCGYC 2 cut(s) 839, 992
AfaI GTAC 1 cut(s) 968
AfiI CCNNNNNNNGG 7 cut(s) 63, 224, 349, 414, 544, 1008, 1107
AgsI TTSAA 4 cut(s) 389, 509, 805, 853
AjiI CACGTC 1 cut(s) 1047
AjnI CCWGG 6 cut(s) 56, 249, 342, 549, 1035, 1076
AluBI AGCT 8 cut(s) 20, 135, 235, 355, 565, 580, 888, 1071
AluI AGCT 8 cut(s) 20, 135, 235, 355, 565, 580, 888, 1071
Alw21I GWGCWC 3 cut(s) 137, 357, 567
Alw26I GTCTC 2 cut(s) 124, 169
AlwI GGATC 2 cut(s) 368, 1081
AlwNI CAGNNNCTG 1 cut(s) 550
Ama87I CYCGRG 4 cut(s) 499, 525, 736, 768
ApeKI GCWGC 1 cut(s) 235
AspLEI GCGC 7 cut(s) 143, 230, 240, 266, 600, 680, 960
AspS9I GGNCC 9 cut(s) 372, 463, 547, 553, 624, 793, 798, 1033, 1060
AsuHPI GGTGA 3 cut(s) 70, 565, 949
AsuII TTCGAA 1 cut(s) 330
AvaI CYCGRG 4 cut(s) 499, 525, 736, 768
AvaII GGWCC 4 cut(s) 463, 793, 1033, 1060
BanII GRGCYC 5 cut(s) 137, 357, 567, 777, 1015
BauI CACGAG 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 811
Bbv12I GWGCWC 3 cut(s) 137, 357, 567
BbvI GCAGC 1 cut(s) 222
BccI CCATC 2 cut(s) 203, 416
BceAI ACGGC 5 cut(s) 241, 332, 359, 590, 715
BcgI CGANNNNNNTGC 2 cut(s) 202, 236
BciT130I CCWGG 6 cut(s) 58, 251, 344, 551, 1037, 1078
BcoDI GTCTC 2 cut(s) 124, 169
BfoI RGCGCY 2 cut(s) 267, 961
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 8 cut(s) 236, 279, 282, 403, 627, 666, 687, 908
BlsI GCNGC 8 cut(s) 237, 280, 283, 404, 628, 667, 688, 909
BmcAI AGTACT 1 cut(s) 968
Bme18I GGWCC 4 cut(s) 463, 793, 1033, 1060
BmeT110I CYCGRG 4 cut(s) 499, 525, 736, 768
BmgBI CACGTC 1 cut(s) 1047
BmgT120I GGNCC 9 cut(s) 372, 463, 547, 553, 624, 793, 798, 1033, 1060
BmiI GGNNCC 6 cut(s) 193, 406, 446, 625, 774, 1061
BmrI ACTGGG 1 cut(s) 550
BmsI GCATC 2 cut(s) 325, 750
BmuI ACTGGG 1 cut(s) 550
BoxI GACNNNNGTC 1 cut(s) 527
BpiI GAAGAC 1 cut(s) 811
Bpu14I TTCGAA 1 cut(s) 330
BpuEI CTTGAG 2 cut(s) 17, 1075
BsaHI GRCGYC 2 cut(s) 839, 992
BsaWI WCCGGW 1 cut(s) 414
BsaXI ACNNNNNCTCC 6 cut(s) 98, 104, 128, 134, 1031, 1061
Bsc4I CCNNNNNNNGG 7 cut(s) 63, 224, 349, 414, 544, 1008, 1107
Bse118I RCCGGY 2 cut(s) 284, 795
Bse1I ACTGG 4 cut(s) 131, 466, 556, 810
BseBI CCWGG 6 cut(s) 58, 251, 344, 551, 1037, 1078
BseGI GGATG 2 cut(s) 408, 708
BseLI CCNNNNNNNGG 7 cut(s) 63, 224, 349, 414, 544, 1008, 1107
BseMII CTCAG 2 cut(s) 557, 1028
BseNI ACTGG 4 cut(s) 131, 466, 556, 810
BseRI GAGGAG 4 cut(s) 20, 157, 422, 1043
BseX3I CGGCCG 1 cut(s) 282
BseXI GCAGC 1 cut(s) 222
Bsh1236I CGCG 5 cut(s) 240, 281, 600, 907, 1106
Bsh1285I CGRYCG 2 cut(s) 285, 370
BsiEI CGRYCG 2 cut(s) 285, 370
BsiHKAI GWGCWC 3 cut(s) 137, 357, 567
BsiHKCI CYCGRG 4 cut(s) 499, 525, 736, 768
BslFI GGGAC 2 cut(s) 671, 850
BslI CCNNNNNNNGG 7 cut(s) 63, 224, 349, 414, 544, 1008, 1107
BsmAI GTCTC 2 cut(s) 124, 169
BsmBI CGTCTC 2 cut(s) 124, 169
BsmFI GGGAC 2 cut(s) 671, 850
BsmI GAATGC 1 cut(s) 1024
BsoBI CYCGRG 4 cut(s) 499, 525, 736, 768
Bsp119I TTCGAA 1 cut(s) 330
Bsp1286I GDGCHC 5 cut(s) 137, 357, 567, 777, 1015
Bsp143I GATC 6 cut(s) 322, 360, 424, 897, 927, 1086
BspCNI CTCAG 2 cut(s) 556, 1027
BspFNI CGCG 5 cut(s) 240, 281, 600, 907, 1106
BspLI GGNNCC 6 cut(s) 193, 406, 446, 625, 774, 1061
BspPI GGATC 2 cut(s) 368, 1081
BspT104I TTCGAA 1 cut(s) 330
BsrFI RCCGGY 2 cut(s) 284, 795
BsrI ACTGG 4 cut(s) 131, 466, 556, 810
BssAI RCCGGY 2 cut(s) 284, 795
BssMI GATC 6 cut(s) 322, 360, 424, 897, 927, 1086
BssNI GRCGYC 2 cut(s) 839, 992
BssSI CACGAG 1 cut(s) 681
BssT1I CCWWGG 2 cut(s) 1063, 1092
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 6 cut(s) 58, 251, 344, 551, 1037, 1078
Bst4CI ACNGT 3 cut(s) 82, 722, 923
BstACI GRCGYC 2 cut(s) 839, 992
BstBI TTCGAA 1 cut(s) 330
BstC8I GCNNGC 5 cut(s) 6, 154, 563, 676, 765
BstDEI CTNAG 3 cut(s) 490, 543, 1014
BstDSI CCRYGG 2 cut(s) 279, 348
BstF5I GGATG 2 cut(s) 408, 708
BstFNI CGCG 5 cut(s) 240, 281, 600, 907, 1106
BstH2I RGCGCY 2 cut(s) 267, 961
BstHHI GCGC 7 cut(s) 143, 230, 240, 266, 600, 680, 960
BstKTI GATC 6 cut(s) 325, 363, 427, 900, 930, 1089
BstMAI GTCTC 2 cut(s) 124, 169
BstMBI GATC 6 cut(s) 322, 360, 424, 897, 927, 1086
BstMCI CGRYCG 2 cut(s) 285, 370
BstMWI GCNNNNNNNGC 9 cut(s) 149, 290, 344, 371, 626, 671, 686, 998, 1019
BstNI CCWGG 6 cut(s) 58, 251, 344, 551, 1037, 1078
BstNSI RCATGY 1 cut(s) 8
BstPAI GACNNNNGTC 1 cut(s) 527
BstUI CGCG 5 cut(s) 240, 281, 600, 907, 1106
BstV1I GCAGC 1 cut(s) 222
BstV2I GAAGAC 1 cut(s) 811
BstZI CGGCCG 1 cut(s) 282
BtgI CCRYGG 2 cut(s) 279, 348
BtgZI GCGATG 2 cut(s) 181, 622
BtrI CACGTC 1 cut(s) 1047
BtsCI GGATG 2 cut(s) 408, 708
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 2 cut(s) 481, 563
Cac8I GCNNGC 5 cut(s) 6, 154, 563, 676, 765
CaiI CAGNNNCTG 1 cut(s) 550
CfoI GCGC 7 cut(s) 143, 230, 240, 266, 600, 680, 960
Cfr10I RCCGGY 2 cut(s) 284, 795
Cfr13I GGNCC 9 cut(s) 372, 463, 547, 553, 624, 793, 798, 1033, 1060
Cfr42I CCGCGG 1 cut(s) 282
Cfr9I CCCGGG 2 cut(s) 499, 768
CpoI CGGWCCG 1 cut(s) 793
CseI GACGC 1 cut(s) 981
Csp6I GTAC 1 cut(s) 967
CspI CGGWCCG 1 cut(s) 793
CviAII CATG 1 cut(s) 5
CviQI GTAC 1 cut(s) 967
DdeI CTNAG 3 cut(s) 490, 543, 1014
DpnI GATC 6 cut(s) 324, 362, 426, 899, 929, 1088
DpnII GATC 6 cut(s) 322, 360, 424, 897, 927, 1086
EaeI YGGCCR 3 cut(s) 282, 908, 999
EagI CGGCCG 1 cut(s) 282
EciI GGCGGA 1 cut(s) 425
Ecl136II GAGCTC 3 cut(s) 135, 355, 565
EclXI CGGCCG 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 1063, 1092
Eco24I GRGCYC 5 cut(s) 137, 357, 567, 777, 1015
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 4 cut(s) 463, 793, 1033, 1060
Eco52I CGGCCG 1 cut(s) 282
Eco53kI GAGCTC 3 cut(s) 135, 355, 565
Eco57I CTGAAG 1 cut(s) 859
Eco88I CYCGRG 4 cut(s) 499, 525, 736, 768
EcoICRI GAGCTC 3 cut(s) 135, 355, 565
EcoO109I RGGNCCY 2 cut(s) 547, 1060
EcoRII CCWGG 6 cut(s) 56, 249, 342, 549, 1035, 1076
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 2 cut(s) 1063, 1092
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 5 cut(s) 137, 357, 567, 777, 1015
ErhI CCWWGG 2 cut(s) 1063, 1092
Esp3I CGTCTC 2 cut(s) 124, 169
FaeI CATG 1 cut(s) 8
FaiI YATR 4 cut(s) 6, 221, 779, 1085
FaqI GGGAC 2 cut(s) 671, 850
FatI CATG 1 cut(s) 4
FauI CCCGC 1 cut(s) 1099
FblI GTMKAC 1 cut(s) 981
Fnu4HI GCNGC 8 cut(s) 236, 279, 282, 403, 627, 666, 687, 908
FokI GGATG 2 cut(s) 395, 695
FriOI GRGCYC 5 cut(s) 137, 357, 567, 777, 1015
Fsp4HI GCNGC 8 cut(s) 236, 279, 282, 403, 627, 666, 687, 908
GlaI GCGC 7 cut(s) 142, 229, 239, 265, 599, 679, 959
GluI GCNGC 8 cut(s) 236, 279, 282, 403, 627, 666, 687, 908
HaeII RGCGCY 2 cut(s) 267, 961
HgaI GACGC 1 cut(s) 981
HhaI GCGC 7 cut(s) 143, 230, 240, 266, 600, 680, 960
Hin1I GRCGYC 2 cut(s) 839, 992
Hin1II CATG 1 cut(s) 8
Hin6I GCGC 7 cut(s) 141, 228, 238, 264, 598, 678, 958
HinP1I GCGC 7 cut(s) 141, 228, 238, 264, 598, 678, 958
HincII GTYRAC 2 cut(s) 610, 982
HindII GTYRAC 2 cut(s) 610, 982
HinfI GANTC 5 cut(s) 95, 528, 734, 902, 944
HphI GGTGA 3 cut(s) 70, 565, 949
Hpy166II GTNNAC 5 cut(s) 478, 610, 940, 982, 1044
Hpy188I TCNGA 5 cut(s) 94, 124, 274, 927, 1017
Hpy188III TCNNGA 7 cut(s) 358, 656, 728, 805, 827, 877, 1054
Hpy8I GTNNAC 5 cut(s) 478, 610, 940, 982, 1044
Hpy99I CGWCG 3 cut(s) 596, 844, 983
HpyAV CCTTC 5 cut(s) 662, 811, 883, 947, 1111
HpyCH4III ACNGT 3 cut(s) 82, 722, 923
HpyCH4IV ACGT 5 cut(s) 591, 744, 839, 978, 1046
HpyCH4V TGCA 3 cut(s) 4, 8, 223
HpyF10VI GCNNNNNNNGC 9 cut(s) 149, 290, 344, 371, 626, 671, 686, 998, 1019
HpyF3I CTNAG 3 cut(s) 490, 543, 1014
HpySE526I ACGT 5 cut(s) 591, 744, 839, 978, 1046
Hsp92I GRCGYC 2 cut(s) 839, 992
Hsp92II CATG 1 cut(s) 8
HspAI GCGC 7 cut(s) 141, 228, 238, 264, 598, 678, 958
KspI CCGCGG 1 cut(s) 282
Kzo9I GATC 6 cut(s) 322, 360, 424, 897, 927, 1086
LmnI GCTCC 6 cut(s) 95, 140, 352, 410, 450, 772
Lsp1109I GCAGC 1 cut(s) 222
LweI GCATC 2 cut(s) 325, 750
MaeII ACGT 5 cut(s) 591, 744, 839, 978, 1046
MaeIII GTNAC 2 cut(s) 468, 745
MalI GATC 6 cut(s) 324, 362, 426, 899, 929, 1088
MboI GATC 6 cut(s) 322, 360, 424, 897, 927, 1086
MboII GAAGA 3 cut(s) 344, 419, 811
MfeI CAATTG 1 cut(s) 384
MhlI GDGCHC 5 cut(s) 137, 357, 567, 777, 1015
MluCI AATT 3 cut(s) 326, 384, 418
MlyI GAGTC 5 cut(s) 89, 537, 728, 911, 938
MmeI TCCRAC 3 cut(s) 117, 147, 669
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 3 cut(s) 20, 235, 281
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 1024
MvaI CCWGG 6 cut(s) 58, 251, 344, 551, 1037, 1078
MvnI CGCG 5 cut(s) 240, 281, 600, 907, 1106
MwoI GCNNNNNNNGC 9 cut(s) 149, 290, 344, 371, 626, 671, 686, 998, 1019
NdeII GATC 6 cut(s) 322, 360, 424, 897, 927, 1086
NlaIII CATG 1 cut(s) 8
NlaIV GGNNCC 6 cut(s) 193, 406, 446, 625, 774, 1061
NmeAIII GCCGAG 4 cut(s) 378, 561, 655, 1027
NmuCI GTSAC 1 cut(s) 745
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
NspV TTCGAA 1 cut(s) 330
PaeI GCATGC 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 736
PasI CCCWGGG 1 cut(s) 1077
PcsI WCGNNNNNNNCGW 4 cut(s) 588, 597, 948, 984
PctI GAATGC 1 cut(s) 1024
PflMI CCANNNNNTGG 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 1035
PkrI GCNGC 8 cut(s) 237, 280, 283, 404, 628, 667, 688, 909
PleI GAGTC 5 cut(s) 89, 536, 728, 910, 938
PpsI GAGTC 5 cut(s) 89, 536, 728, 910, 938
PpuMI RGGWCCY 1 cut(s) 1060
PshAI GACNNNNGTC 1 cut(s) 527
Psp124BI GAGCTC 3 cut(s) 137, 357, 567
Psp5II RGGWCCY 1 cut(s) 1060
Psp6I CCWGG 6 cut(s) 56, 249, 342, 549, 1035, 1076
PspGI CCWGG 6 cut(s) 56, 249, 342, 549, 1035, 1076
PspN4I GGNNCC 6 cut(s) 193, 406, 446, 625, 774, 1061
PspPI GGNCC 9 cut(s) 372, 463, 547, 553, 624, 793, 798, 1033, 1060
PspPPI RGGWCCY 1 cut(s) 1060
PspXI VCTCGAGB 1 cut(s) 736
PstNI CAGNNNCTG 1 cut(s) 550
PvuII CAGCTG 2 cut(s) 20, 235
RsaI GTAC 1 cut(s) 968
RsaNI GTAC 1 cut(s) 967
Rsr2I CGGWCCG 1 cut(s) 793
RsrII CGGWCCG 1 cut(s) 793
SacI GAGCTC 3 cut(s) 137, 357, 567
SacII CCGCGG 1 cut(s) 282
SalI GTCGAC 1 cut(s) 980
SatI GCNGC 8 cut(s) 236, 279, 282, 403, 627, 666, 687, 908
Sau3AI GATC 6 cut(s) 322, 360, 424, 897, 927, 1086
Sau96I GGNCC 9 cut(s) 372, 463, 547, 553, 624, 793, 798, 1033, 1060
ScaI AGTACT 1 cut(s) 968
SchI GAGTC 5 cut(s) 89, 537, 728, 911, 938
SduI GDGCHC 5 cut(s) 137, 357, 567, 777, 1015
SfaNI GCATC 2 cut(s) 325, 750
Sfr274I CTCGAG 1 cut(s) 736
Sfr303I CCGCGG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 330
SgrBI CCGCGG 1 cut(s) 282
SinI GGWCC 4 cut(s) 463, 793, 1033, 1060
SlaI CTCGAG 1 cut(s) 736
SmaI CCCGGG 2 cut(s) 501, 770
SmlI CTYRAG 3 cut(s) 32, 736, 1054
SmoI CTYRAG 3 cut(s) 32, 736, 1054
SphI GCATGC 1 cut(s) 8
Sse9I AATT 3 cut(s) 326, 384, 418
SstI GAGCTC 3 cut(s) 137, 357, 567
StyI CCWWGG 2 cut(s) 1063, 1092
TaaI ACNGT 3 cut(s) 82, 722, 923
TaiI ACGT 5 cut(s) 594, 747, 842, 981, 1049
TaqI TCGA 8 cut(s) 330, 594, 737, 900, 947, 963, 981, 987
TasI AATT 3 cut(s) 326, 384, 418
TatI WGTACW 1 cut(s) 966
TauI GCSGC 7 cut(s) 281, 284, 405, 629, 668, 689, 910
TscAI CASTG 2 cut(s) 488, 563
TseFI GTSAC 1 cut(s) 745
TseI GCWGC 1 cut(s) 235
Tsp45I GTSAC 1 cut(s) 745
TspDTI ATGAA 1 cut(s) 625
TspGWI ACGGA 2 cut(s) 106, 806
TspMI CCCGGG 2 cut(s) 499, 768
TspRI CASTG 2 cut(s) 488, 563
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 4 cut(s) 463, 793, 1033, 1060
XceI RCATGY 1 cut(s) 8
XhoI CTCGAG 1 cut(s) 736
XmaI CCCGGG 2 cut(s) 499, 768
XmiI GTMKAC 1 cut(s) 981
ZraI GACGTC 1 cut(s) 840
ZrmI AGTACT 1 cut(s) 968
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.