FvH4_5g29110

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015884
Physical Location & Seq
Forward (+)
20196018 .. 20198415
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29110.t1

Sequence Viewer

Length: 1245 bp
ATGCTCGGAATATCCAACATTTGCAAGGGATACAAATCCCTTGTTATGAAATGGAACACCCAACACAAGCATTCTGCCTCTATGGACAGTAATGAAACATCCAGGGAGAAAGATGAGAATGATTTTATAATTAGTTATCCATCGACACCAGTAGGTGGTGGTTTGCATCATACGCACAGCATTGATGATAGCTTACTCTCCCGTCGAACTCTTTCGAGGAATCAGAGTAGCAGAAGATGCAAGACCCCTACGCCAAGGTCCTTCTCCAGAAGTGTGAGCCGGAGCACCACCTCATCATCGGCAATTACAAGTCGTAGGAACCCATCCGAGACCGATATCCTTGCCTCTATAACAAGAAATCTGAGTCGGGAGGCTGAACTTGCTGCCTCTATATCAAGGAATATAAGTAGGAACATGAGTAGCAATGCAAGCTGCAGTTTGAACAGGACTAAGAGCCAGAATAATGGGAGCAGGCGAAACCCGTCTGAGTCTGATTTACCCTCTCCATCACCTTTATCTCCACCGCGCAGCTCCCCACCGGCCTCACCTTCACCTGCCGCCTCTCCATCTACCGCTCGATCACCTCGTACCAGTAAAACATCTGAAACGGACCGTACACCACTCACAAGAACCCCGAGCAGGAGGAGCACCACCCCCATTGTGTTCTCTCAGACAACGGCGAGAAGGAAACCTCCCCCGGTTGAGAAGAAGCTTGAATTCACACTCGAAGAGTTGTGTCATGGATGCGTAAAGAAGATCAAGGTTACTAAAGATGTCATCAACCATGCAGGGATTATTGTGAAAGAAGAGGAAATGCTGAAAATAAATGTGCAGCCAGGTTGGAGGAAAGGAACCAAGATTACTTTCGAAGGGAAAGGGGATGAGAAACCAGGGTACCTTCCAGCTGATATCATCTTCTTGATAGATGAAAAGAGGCATCATTTGTTCAAAAGAGCAGGCCGGGACGATTTGGAAATCGCTGTTGAGATTCCGCTGGCTGATGCATTGGGAGGCTGCTCGTTTCCAGCTCCCTTGCTAGGAGGTCAAAAAATGAATCTGTCATTCGACCACATCATACATCATGGTTATGAAAAGGTCATTGAAGGACAAGGGATGCCAAGGCTTAAAGAACCAAAAAAGAGAGGTGACCTCCGCATTACTTGTCTCGTTAAATTTCCCAAAAAATTGAGCAACGAGCAACGAGCAGAAGCTGTTAGAATTTTACAAGATTGTTCTTATCAGTAA

Protein Analysis

415

Amino Acids

45.91

Weight (kDa)

9.89

Isoelectric Point (pI)

71.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 235 - 393 2.5e-34 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11040
fragaria_vesca FvH4_5g29110
malus_domestica MD08G1235600.v1.1 MD15G1427300.v1.1
prunus_persica Prupe.1G571300_v2.0.a1
pyrus_communis pycom15g37880
rosa_chinensis RchiOBHm_Chr7g0221771
rosa_laevigata RLG00000002125
rosa_roxburghii Rroxscaffold_3G00237740
rosa_rugosa Rorug07G0203200
rosa_samantha Rh7AG344100 Rh7BG334800 Rh7CG361500 Rh7DG341900
rosa_wichuraiana Rw7G029180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
AarI CACCTGC 1 cut(s) 562
Acc36I ACCTGC 1 cut(s) 562
Acc65I GGTACC 1 cut(s) 894
AccB1I GGYRCC 1 cut(s) 894
AccB7I CCANNNNNTGG 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 575
AccII CGCG 1 cut(s) 526
AciI CCGC 5 cut(s) 524, 558, 573, 992, 1153
AcsI RAATTY 3 cut(s) 716, 1172, 1218
AfaI GTAC 3 cut(s) 589, 616, 896
AfiI CCNNNNNNNGG 3 cut(s) 155, 639, 1037
AgsI TTSAA 4 cut(s) 442, 716, 949, 1103
AjnI CCWGG 3 cut(s) 101, 835, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AluBI AGCT 7 cut(s) 192, 432, 531, 712, 905, 1028, 1211
AluI AGCT 7 cut(s) 192, 432, 531, 712, 905, 1028, 1211
Alw21I GWGCWC 2 cut(s) 287, 650
Alw26I GTCTC 2 cut(s) 323, 1169
AlwNI CAGNNNCTG 1 cut(s) 1211
Ama87I CYCGRG 1 cut(s) 634
AoxI GGCC 2 cut(s) 540, 958
ApeKI GCWGC 5 cut(s) 383, 432, 528, 832, 1014
ApoI RAATTY 3 cut(s) 716, 1172, 1218
Asp700I GAANNNNTTC 1 cut(s) 211
Asp718I GGTACC 1 cut(s) 894
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 2 cut(s) 258, 610
AsuC2I CCSGG 2 cut(s) 698, 962
AsuHPI GGTGA 5 cut(s) 501, 537, 543, 573, 1157
AsuII TTCGAA 1 cut(s) 867
AvaI CYCGRG 1 cut(s) 634
AvaII GGWCC 2 cut(s) 258, 610
BaeI ACNNNNGTAYC 2 cut(s) 878, 911
BanI GGYRCC 1 cut(s) 894
Bbv12I GWGCWC 2 cut(s) 287, 650
BbvI GCAGC 5 cut(s) 370, 419, 540, 844, 1001
BccI CCATC 4 cut(s) 148, 331, 514, 574
BceAI ACGGC 1 cut(s) 693
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 3 cut(s) 103, 837, 891
BciVI GTATCC 1 cut(s) 23
BcnI CCSGG 2 cut(s) 698, 962
BcoDI GTCTC 2 cut(s) 323, 1169
BfaI CTAG 1 cut(s) 1037
BfmI CTRYAG 1 cut(s) 433
BfuAI ACCTGC 1 cut(s) 562
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 6 cut(s) 384, 433, 529, 558, 833, 1015
BlsI GCNGC 6 cut(s) 385, 434, 530, 559, 834, 1016
Bme1390I CCNGG 5 cut(s) 103, 698, 837, 891, 962
Bme18I GGWCC 2 cut(s) 258, 610
BmeT110I CYCGRG 1 cut(s) 634
BmgT120I GGNCC 2 cut(s) 258, 610
BmiI GGNNCC 3 cut(s) 320, 853, 896
BmrFI CCNGG 5 cut(s) 103, 698, 837, 891, 962
BmsI GCATC 6 cut(s) 175, 227, 734, 946, 991, 1104
BplI GAGNNNNNCTC 2 cut(s) 1134, 1166
BpmI CTGGAG 1 cut(s) 250
Bpu14I TTCGAA 1 cut(s) 867
BpuMI CCSGG 2 cut(s) 698, 962
BsaBI GATNNNNATC 1 cut(s) 34
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 5 cut(s) 102, 254, 696, 890, 1118
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 639, 1037
Bse118I RCCGGY 1 cut(s) 538
Bse1I ACTGG 2 cut(s) 149, 591
Bse3DI GCAATG 1 cut(s) 430
Bse8I GATNNNNATC 1 cut(s) 34
BseBI CCWGG 3 cut(s) 103, 837, 891
BseDI CCNNGG 5 cut(s) 102, 254, 696, 890, 1118
BseGI GGATG 5 cut(s) 98, 323, 749, 886, 1119
BseJI GATNNNNATC 1 cut(s) 34
BseLI CCNNNNNNNGG 3 cut(s) 155, 639, 1037
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 3 cut(s) 353, 477, 683
BseNI ACTGG 2 cut(s) 149, 591
BseRI GAGGAG 1 cut(s) 658
BseXI GCAGC 5 cut(s) 370, 419, 540, 844, 1001
BsgI GTGCAG 1 cut(s) 851
Bsh1236I CGCG 1 cut(s) 526
BshFI GGCC 2 cut(s) 542, 960
BshNI GGYRCC 1 cut(s) 894
BsiHKAI GWGCWC 2 cut(s) 287, 650
BsiHKCI CYCGRG 1 cut(s) 634
BsiSI CCGG 4 cut(s) 280, 539, 698, 961
BslFI GGGAC 1 cut(s) 977
BslI CCNNNNNNNGG 3 cut(s) 155, 639, 1037
BsmAI GTCTC 2 cut(s) 323, 1169
BsmFI GGGAC 1 cut(s) 977
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 2 cut(s) 542, 960
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 634
Bsp119I TTCGAA 1 cut(s) 867
Bsp1286I GDGCHC 2 cut(s) 287, 650
Bsp143I GATC 2 cut(s) 578, 756
BspACI CCGC 5 cut(s) 524, 558, 573, 992, 1153
BspANI GGCC 2 cut(s) 542, 960
BspCNI CTCAG 3 cut(s) 354, 478, 682
BspFNI CGCG 1 cut(s) 526
BspLI GGNNCC 3 cut(s) 320, 853, 896
BspMAI CTGCAG 1 cut(s) 437
BspMI ACCTGC 1 cut(s) 562
BspT104I TTCGAA 1 cut(s) 867
BspT107I GGYRCC 1 cut(s) 894
BspTNI GGTCTC 1 cut(s) 323
BsrBI CCGCTC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 430
BsrFI RCCGGY 1 cut(s) 538
BsrI ACTGG 2 cut(s) 149, 591
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 5 cut(s) 102, 254, 696, 890, 1118
BssMI GATC 2 cut(s) 578, 756
BssT1I CCWWGG 2 cut(s) 254, 1118
Bst2UI CCWGG 3 cut(s) 103, 837, 891
Bst4CI ACNGT 2 cut(s) 89, 614
Bst6I CTCTTC 2 cut(s) 723, 801
BstAPI GCANNNNNTGC 1 cut(s) 237
BstBI TTCGAA 1 cut(s) 867
BstC8I GCNNGC 4 cut(s) 430, 473, 958, 996
BstDEI CTNAG 4 cut(s) 362, 450, 486, 669
BstEII GGTNACC 1 cut(s) 1145
BstF5I GGATG 5 cut(s) 98, 323, 749, 886, 1119
BstFNI CGCG 1 cut(s) 526
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 2 cut(s) 581, 759
BstMAI GTCTC 2 cut(s) 323, 1169
BstMBI GATC 2 cut(s) 578, 756
BstMWI GCNNNNNNNGC 5 cut(s) 172, 237, 380, 429, 645
BstNI CCWGG 3 cut(s) 103, 837, 891
BstPI GGTNACC 1 cut(s) 1145
BstSCI CCNGG 5 cut(s) 101, 696, 835, 889, 960
BstSFI CTRYAG 1 cut(s) 433
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 5 cut(s) 370, 419, 540, 844, 1001
BstXI CCANNNNNNTGG 1 cut(s) 464
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 542, 960
BtsCI GGATG 5 cut(s) 98, 323, 749, 886, 1119
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 4 cut(s) 430, 473, 958, 996
CaiI CAGNNNCTG 1 cut(s) 1211
CfoI GCGC 1 cut(s) 528
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 2 cut(s) 258, 610
CpoI CGGWCCG 1 cut(s) 610
Csp6I GTAC 3 cut(s) 588, 615, 895
CspCI CAANNNNNGTGG 2 cut(s) 640, 675
CspI CGGWCCG 1 cut(s) 610
CviAII CATG 4 cut(s) 415, 740, 785, 1082
CviQI GTAC 3 cut(s) 588, 615, 895
DdeI CTNAG 4 cut(s) 362, 450, 486, 669
DpnI GATC 2 cut(s) 580, 758
DpnII GATC 2 cut(s) 578, 756
Eam1104I CTCTTC 2 cut(s) 723, 801
EarI CTCTTC 2 cut(s) 723, 801
Eco130I CCWWGG 2 cut(s) 254, 1118
Eco31I GGTCTC 1 cut(s) 323
Eco32I GATATC 2 cut(s) 337, 910
Eco47I GGWCC 2 cut(s) 258, 610
Eco88I CYCGRG 1 cut(s) 634
Eco91I GGTNACC 1 cut(s) 1145
EcoO109I RGGNCCY 1 cut(s) 258
EcoO65I GGTNACC 1 cut(s) 1145
EcoRI GAATTC 1 cut(s) 716
EcoRII CCWGG 3 cut(s) 101, 835, 889
EcoRV GATATC 2 cut(s) 337, 910
EcoT14I CCWWGG 2 cut(s) 254, 1118
EcoT22I ATGCAT 1 cut(s) 1006
ErhI CCWWGG 2 cut(s) 254, 1118
FaeI CATG 4 cut(s) 418, 743, 788, 1085
FaqI GGGAC 1 cut(s) 977
FatI CATG 4 cut(s) 414, 739, 784, 1081
Fnu4HI GCNGC 6 cut(s) 384, 433, 529, 558, 833, 1015
FokI GGATG 5 cut(s) 85, 310, 756, 893, 1126
Fsp4HI GCNGC 6 cut(s) 384, 433, 529, 558, 833, 1015
FspBI CTAG 1 cut(s) 1037
GlaI GCGC 1 cut(s) 527
GluI GCNGC 6 cut(s) 384, 433, 529, 558, 833, 1015
GsuI CTGGAG 1 cut(s) 250
HaeIII GGCC 2 cut(s) 542, 960
HapII CCGG 4 cut(s) 280, 539, 698, 961
HhaI GCGC 1 cut(s) 528
Hin1II CATG 4 cut(s) 418, 743, 788, 1085
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 5 cut(s) 220, 364, 488, 988, 1054
HpaII CCGG 4 cut(s) 280, 539, 698, 961
HphI GGTGA 5 cut(s) 501, 537, 543, 573, 1157
Hpy166II GTNNAC 1 cut(s) 617
Hpy188I TCNGA 8 cut(s) 8, 225, 328, 363, 487, 493, 604, 672
Hpy188III TCNNGA 3 cut(s) 267, 368, 919
Hpy8I GTNNAC 1 cut(s) 617
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 6 cut(s) 271, 558, 678, 863, 908, 1097
HpyCH4III ACNGT 2 cut(s) 89, 614
HpyCH4V TGCA 8 cut(s) 24, 166, 240, 428, 435, 788, 832, 1004
HpyF10VI GCNNNNNNNGC 5 cut(s) 172, 237, 380, 429, 645
HpyF3I CTNAG 4 cut(s) 362, 450, 486, 669
Hsp92II CATG 4 cut(s) 418, 743, 788, 1085
HspAI GCGC 1 cut(s) 526
KpnI GGTACC 1 cut(s) 898
Kzo9I GATC 2 cut(s) 578, 756
LmnI GCTCC 5 cut(s) 282, 468, 536, 645, 1033
Lsp1109I GCAGC 5 cut(s) 370, 419, 540, 844, 1001
LweI GCATC 6 cut(s) 175, 227, 734, 946, 991, 1104
MaeI CTAG 1 cut(s) 1037
MaeIII GTNAC 2 cut(s) 763, 1145
MalI GATC 2 cut(s) 580, 758
MbiI CCGCTC 1 cut(s) 575
MboI GATC 2 cut(s) 578, 756
MboII GAAGA 6 cut(s) 246, 718, 740, 766, 818, 907
MhlI GDGCHC 2 cut(s) 287, 650
MluCI AATT 6 cut(s) 129, 303, 716, 1172, 1184, 1218
MlyI GAGTC 2 cut(s) 373, 497
MmeI TCCRAC 2 cut(s) 39, 821
Mph1103I ATGCAT 1 cut(s) 1006
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 2 cut(s) 1125, 1170
MslI CAYNNNNRTG 1 cut(s) 1086
MspA1I CMGCKG 2 cut(s) 905, 994
MspI CCGG 4 cut(s) 280, 539, 698, 961
MspR9I CCNGG 5 cut(s) 103, 698, 837, 891, 962
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 3 cut(s) 103, 837, 891
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 5 cut(s) 172, 237, 380, 429, 645
NciI CCSGG 2 cut(s) 698, 962
NdeII GATC 2 cut(s) 578, 756
NlaIII CATG 4 cut(s) 418, 743, 788, 1085
NlaIV GGNNCC 3 cut(s) 320, 853, 896
NmuCI GTSAC 1 cut(s) 1145
NsiI ATGCAT 1 cut(s) 1006
NspV TTCGAA 1 cut(s) 867
PaqCI CACCTGC 1 cut(s) 562
PcsI WCGNNNNNNNCGW 1 cut(s) 583
PctI GAATGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 3 cut(s) 220, 988, 1054
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 6 cut(s) 385, 434, 530, 559, 834, 1016
PleI GAGTC 2 cut(s) 372, 496
PpsI GAGTC 2 cut(s) 372, 496
PpuMI RGGWCCY 1 cut(s) 258
PsiI TTATAA 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 101, 835, 889
PspEI GGTNACC 1 cut(s) 1145
PspGI CCWGG 3 cut(s) 101, 835, 889
PspN4I GGNNCC 3 cut(s) 320, 853, 896
PspPI GGNCC 2 cut(s) 258, 610
PspPPI RGGWCCY 1 cut(s) 258
PsrI GAACNNNNNNTAC 2 cut(s) 844, 876
PstI CTGCAG 1 cut(s) 437
PstNI CAGNNNCTG 1 cut(s) 1211
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 3 cut(s) 589, 616, 896
RsaNI GTAC 3 cut(s) 588, 615, 895
RseI CAYNNNNRTG 1 cut(s) 1086
Rsr2I CGGWCCG 1 cut(s) 610
RsrII CGGWCCG 1 cut(s) 610
SaqAI TTAA 2 cut(s) 1125, 1170
SatI GCNGC 6 cut(s) 384, 433, 529, 558, 833, 1015
Sau3AI GATC 2 cut(s) 578, 756
Sau96I GGNCC 2 cut(s) 258, 610
SchI GAGTC 2 cut(s) 373, 497
ScrFI CCNGG 5 cut(s) 103, 698, 837, 891, 962
SduI GDGCHC 2 cut(s) 287, 650
SfaNI GCATC 6 cut(s) 175, 227, 734, 946, 991, 1104
SfcI CTRYAG 1 cut(s) 433
SfuI TTCGAA 1 cut(s) 867
SinI GGWCC 2 cut(s) 258, 610
SmiMI CAYNNNNRTG 1 cut(s) 1086
Sse9I AATT 6 cut(s) 129, 303, 716, 1172, 1184, 1218
SsiI CCGC 5 cut(s) 524, 558, 573, 992, 1153
SspMI CTAG 1 cut(s) 1037
StyD4I CCNGG 5 cut(s) 101, 696, 835, 889, 960
StyI CCWWGG 2 cut(s) 254, 1118
TaaI ACNGT 2 cut(s) 89, 614
TaqI TCGA 7 cut(s) 143, 205, 215, 577, 726, 867, 1065
TaqII GACCGA 1 cut(s) 347
TasI AATT 6 cut(s) 129, 303, 716, 1172, 1184, 1218
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 3 cut(s) 220, 988, 1054
Tru1I TTAA 2 cut(s) 1125, 1170
Tru9I TTAA 2 cut(s) 1125, 1170
TseFI GTSAC 1 cut(s) 1145
TseI GCWGC 5 cut(s) 383, 432, 528, 832, 1014
Tsp45I GTSAC 1 cut(s) 1145
TspDTI ATGAA 5 cut(s) 62, 108, 942, 1067, 1104
TspGWI ACGGA 1 cut(s) 623
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 2 cut(s) 258, 610
XapI RAATTY 3 cut(s) 716, 1172, 1218
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 1 cut(s) 1037
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.