MD08G1235600.v1.1

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015884
Physical Location & Seq
Reverse (-)
30308629 .. 30310393
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1235600.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGGGGGATCGTCCACGTTCAGCCTCGACGTCGGATCTCTACAACATGCTCGGAATCTCGAATATTTGCAAAGGATACAAATCCTTTGTTATGAAATTGGCCCCCAACAATAAGAATGGAGAATCCAAGTTTACGGACAATCACGACACCAACAAGGAGGACGAAAATTTTACGATTAGTTATCCAACGACACCGGTAGGCAGTTTCGAACGAAGTGTTGATGATAACTTCCTCTCCCGCCGAAGTCTTTCAAGAACAACGAGTAGAAGATCTAAGACACCTACACCGATGAGCTTCTCAAGATGTGCAAGCCGGAGCACCTCTCCGACATCCTCATCATTATCGAGAATTATGAGTCGAAGAAATGCCTCTGAGACCGAAATCTCTGCCCCTATTTCGAGAAATAGGGGTCGAAGGAGCACTTCAGAGATTGACATTCCTTCCCTTACATCAAGAAATATGAGCCGGAGGCACCCATCTGAGACTGAAATACCTTTACCATCACCCACCGCCGCTTCAGCACCCACCACTCCATCACCGGTCTCTCCATCGCCAACCTCTCAATCATCAAACGCCAGTAAATCAGGCACGTCAGATCAACCGGAGTCTTTATCAAGAAGCGTGAGCAGGAGGAGCACAACCCCCATTGTGTTCTCTCGAACAACTGCAAGAAAGAAACCTCTTCCGATCGAGAAGAAGCTGGCATTCACACTTGAAGAGTTGTGTCACGGATGTTTAAAAAAGATCAAGATCACAAGAGATGTAATCAACAATGCCGGAATTATTGTCCAAGAAGAGGAAACACTGAAAATAAACGTGCAGCCTGGATGGAGGAAAGGAACGAAGATTACATTTGAAGCGAAAGGTGATGAGAAACCAGGGTACCTCCCAGCCGACATAGTCTTCTTGATAGATGAAAAGAGGCACAATCTGTTTAAAAGATCAGGCCGCGACGATTTGGAAATCGCTGTTGAGATCCCTCTGGCAGATGCACTGGGAGGCTGCTCATTTCCAGTTCCTCTGCTAGGAGGAGACAAAATGAGACTTTCATTAGACGACATCGTAAATCATGGCTATGAAAAAGTCATTCCAGGACAGGGAATGCCAAAGCTTAAAGAGCCAACAAAGAGAGGCGACCTCCGGATCACATGTCTCGTAAATTTTCCAACACAGTTGAGTGACGAGAACCTAGCAGAAGCAGTTCAAATTCTACAAGATTGTTCTTATGATTAA

Protein Analysis

411

Amino Acids

45.23

Weight (kDa)

9.35

Isoelectric Point (pI)

69.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 230 - 389 1.8e-34 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11040
fragaria_vesca FvH4_5g29110
malus_domestica MD08G1235600.v1.1 MD15G1427300.v1.1
prunus_persica Prupe.1G571300_v2.0.a1
pyrus_communis pycom15g37880
rosa_chinensis RchiOBHm_Chr7g0221771
rosa_laevigata RLG00000002125
rosa_roxburghii Rroxscaffold_3G00237740
rosa_rugosa Rorug07G0203200
rosa_samantha Rh7AG344100 Rh7BG334800 Rh7CG361500 Rh7DG341900
rosa_wichuraiana Rw7G029180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 32
Acc65I GGTACC 1 cut(s) 882
AccB1I GGYRCC 2 cut(s) 471, 882
AccII CGCG 1 cut(s) 951
AccIII TCCGGA 1 cut(s) 1140
AciI CCGC 4 cut(s) 238, 510, 513, 949
AclWI GGATC 4 cut(s) 15, 42, 970, 1151
AcsI RAATTY 3 cut(s) 166, 1159, 1206
AcuI CTGAAG 2 cut(s) 408, 501
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 1 cut(s) 884
AfiI CCNNNNNNNGG 3 cut(s) 796, 1025, 1097
AflIII ACRYGT 1 cut(s) 1148
AgeI ACCGGT 2 cut(s) 193, 538
AgsI TTSAA 4 cut(s) 252, 716, 857, 1205
AjiI CACGTC 1 cut(s) 591
AjnI CCWGG 3 cut(s) 823, 877, 1090
AluBI AGCT 3 cut(s) 294, 700, 1111
AluI AGCT 3 cut(s) 294, 700, 1111
Alw21I GWGCWC 3 cut(s) 320, 422, 638
Alw26I GTCTC 6 cut(s) 368, 476, 547, 1026, 1036, 1157
AlwI GGATC 4 cut(s) 15, 42, 970, 1151
Aor13HI TCCGGA 1 cut(s) 1140
AoxI GGCC 2 cut(s) 99, 946
ApeKI GCWGC 2 cut(s) 820, 1002
ApoI RAATTY 3 cut(s) 166, 1159, 1206
AsiGI ACCGGT 2 cut(s) 193, 538
Asp700I GAANNNNTTC 2 cut(s) 247, 1200
Asp718I GGTACC 1 cut(s) 882
AspS9I GGNCC 1 cut(s) 100
AsuHPI GGTGA 3 cut(s) 495, 528, 878
AsuII TTCGAA 1 cut(s) 207
BaeI ACNNNNGTAYC 2 cut(s) 866, 899
BanI GGYRCC 2 cut(s) 471, 882
BbsI GAAGAC 1 cut(s) 895
Bbv12I GWGCWC 3 cut(s) 320, 422, 638
BbvI GCAGC 2 cut(s) 832, 989
BccI CCATC 5 cut(s) 484, 508, 541, 556, 822
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 3 cut(s) 825, 879, 1092
BciVI GTATCC 1 cut(s) 68
BcoDI GTCTC 6 cut(s) 368, 476, 547, 1026, 1036, 1157
BfaI CTAG 2 cut(s) 1025, 1190
BfuI GTATCC 1 cut(s) 68
BglII AGATCT 1 cut(s) 269
BisI GCNGC 4 cut(s) 513, 821, 949, 1003
BlsI GCNGC 4 cut(s) 514, 822, 950, 1004
Bme1390I CCNGG 3 cut(s) 825, 879, 1092
BmgBI CACGTC 1 cut(s) 591
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 3 cut(s) 102, 473, 884
BmrFI CCNGG 3 cut(s) 825, 879, 1092
BmrI ACTGGG 1 cut(s) 1004
BmsI GCATC 1 cut(s) 979
BmuI ACTGGG 1 cut(s) 1004
BpiI GAAGAC 1 cut(s) 895
BplI GAGNNNNNCTC 4 cut(s) 307, 339, 1122, 1154
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 283
BsaBI GATNNNNATC 2 cut(s) 79, 749
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 2 cut(s) 368, 547
BsaJI CCNNGG 1 cut(s) 878
BsaWI WCCGGW 4 cut(s) 193, 538, 601, 1140
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 796, 1025, 1097
Bse118I RCCGGY 2 cut(s) 193, 538
Bse1I ACTGG 3 cut(s) 576, 999, 1013
Bse8I GATNNNNATC 2 cut(s) 79, 749
BseAI TCCGGA 1 cut(s) 1140
BseBI CCWGG 3 cut(s) 825, 879, 1092
BseDI CCNNGG 1 cut(s) 878
BseGI GGATG 3 cut(s) 329, 737, 833
BseJI GATNNNNATC 2 cut(s) 79, 749
BseLI CCNNNNNNNGG 3 cut(s) 796, 1025, 1097
BseMII CTCAG 2 cut(s) 363, 471
BseNI ACTGG 3 cut(s) 576, 999, 1013
BseRI GAGGAG 2 cut(s) 646, 1044
BseXI GCAGC 2 cut(s) 832, 989
BseYI CCCAGC 1 cut(s) 889
BsgI GTGCAG 1 cut(s) 839
Bsh1236I CGCG 1 cut(s) 951
Bsh1285I CGRYCG 1 cut(s) 690
BshFI GGCC 2 cut(s) 101, 948
BshNI GGYRCC 2 cut(s) 471, 882
BshTI ACCGGT 2 cut(s) 193, 538
BsiEI CGRYCG 1 cut(s) 690
BsiHKAI GWGCWC 3 cut(s) 320, 422, 638
BsiSI CCGG 7 cut(s) 194, 313, 466, 539, 602, 777, 1141
BslI CCNNNNNNNGG 3 cut(s) 796, 1025, 1097
BsmAI GTCTC 6 cut(s) 368, 476, 547, 1026, 1036, 1157
BsmI GAATGC 2 cut(s) 704, 1107
BsnI GGCC 2 cut(s) 101, 948
Bso31I GGTCTC 2 cut(s) 368, 547
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 3 cut(s) 320, 422, 638
Bsp13I TCCGGA 1 cut(s) 1140
BspACI CCGC 4 cut(s) 238, 510, 513, 949
BspANI GGCC 2 cut(s) 101, 948
BspCNI CTCAG 2 cut(s) 364, 472
BspEI TCCGGA 1 cut(s) 1140
BspFNI CGCG 1 cut(s) 951
BspLI GGNNCC 3 cut(s) 102, 473, 884
BspPI GGATC 4 cut(s) 15, 42, 970, 1151
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 2 cut(s) 471, 882
BspTNI GGTCTC 2 cut(s) 368, 547
BsrFI RCCGGY 2 cut(s) 193, 538
BsrI ACTGG 3 cut(s) 576, 999, 1013
BssAI RCCGGY 2 cut(s) 193, 538
BssECI CCNNGG 1 cut(s) 878
BssNI GRCGYC 1 cut(s) 29
Bst2UI CCWGG 3 cut(s) 825, 879, 1092
Bst4CI ACNGT 1 cut(s) 1173
Bst6I CTCTTC 3 cut(s) 687, 711, 789
BstACI GRCGYC 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 310, 702
BstDEI CTNAG 3 cut(s) 273, 372, 480
BstENI CCTNNNNNAGG 1 cut(s) 1023
BstF5I GGATG 3 cut(s) 329, 737, 833
BstFNI CGCG 1 cut(s) 951
BstMAI GTCTC 6 cut(s) 368, 476, 547, 1026, 1036, 1157
BstMCI CGRYCG 1 cut(s) 690
BstMWI GCNNNNNNNGC 3 cut(s) 518, 633, 1117
BstNI CCWGG 3 cut(s) 825, 879, 1092
BstNSI RCATGY 2 cut(s) 49, 1152
BstSCI CCNGG 3 cut(s) 823, 877, 1090
BstUI CGCG 1 cut(s) 951
BstV1I GCAGC 2 cut(s) 832, 989
BstV2I GAAGAC 1 cut(s) 895
BstX2I RGATCY 3 cut(s) 34, 269, 975
BstYI RGATCY 3 cut(s) 34, 269, 975
BsuI GTATCC 1 cut(s) 68
BsuRI GGCC 2 cut(s) 101, 948
BtgZI GCGATG 1 cut(s) 534
BtrI CACGTC 1 cut(s) 591
BtsCI GGATG 3 cut(s) 329, 737, 833
BtsIMutI CAGTG 2 cut(s) 803, 992
Cac8I GCNNGC 2 cut(s) 310, 702
Cfr10I RCCGGY 2 cut(s) 193, 538
Cfr13I GGNCC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 883
CspAI ACCGGT 2 cut(s) 193, 538
CviAII CATG 3 cut(s) 46, 1070, 1149
CviQI GTAC 1 cut(s) 883
DdeI CTNAG 3 cut(s) 273, 372, 480
DraI TTTAAA 2 cut(s) 738, 937
Eam1104I CTCTTC 3 cut(s) 687, 711, 789
EarI CTCTTC 3 cut(s) 687, 711, 789
Eco31I GGTCTC 2 cut(s) 368, 547
Eco57I CTGAAG 2 cut(s) 408, 501
EcoNI CCTNNNNNAGG 1 cut(s) 1023
EcoRII CCWGG 3 cut(s) 823, 877, 1090
FaeI CATG 3 cut(s) 49, 1073, 1152
FaiI YATR 9 cut(s) 47, 92, 353, 461, 899, 1071, 1077, 1150, 1227
FalI AAGNNNNNCTT 2 cut(s) 406, 438
FatI CATG 3 cut(s) 45, 1069, 1148
FauI CCCGC 1 cut(s) 245
Fnu4HI GCNGC 4 cut(s) 513, 821, 949, 1003
FokI GGATG 3 cut(s) 316, 744, 840
Fsp4HI GCNGC 4 cut(s) 513, 821, 949, 1003
FspBI CTAG 2 cut(s) 1025, 1190
GluI GCNGC 4 cut(s) 513, 821, 949, 1003
GsaI CCCAGC 1 cut(s) 893
HaeIII GGCC 2 cut(s) 101, 948
HapII CCGG 7 cut(s) 194, 313, 466, 539, 602, 777, 1141
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 3 cut(s) 49, 1073, 1152
HindIII AAGCTT 1 cut(s) 1109
HinfI GANTC 4 cut(s) 54, 122, 355, 605
HpaII CCGG 7 cut(s) 194, 313, 466, 539, 602, 777, 1141
HphI GGTGA 3 cut(s) 495, 528, 878
Hpy166II GTNNAC 2 cut(s) 14, 132
Hpy188I TCNGA 8 cut(s) 34, 53, 327, 373, 427, 481, 595, 687
Hpy8I GTNNAC 2 cut(s) 14, 132
Hpy99I CGWCG 3 cut(s) 31, 34, 956
HpyAV CCTTC 2 cut(s) 408, 450
HpyCH4III ACNGT 1 cut(s) 1173
HpyCH4IV ACGT 4 cut(s) 16, 29, 590, 816
HpyCH4V TGCA 5 cut(s) 69, 308, 668, 820, 992
HpyF10VI GCNNNNNNNGC 3 cut(s) 518, 633, 1117
HpyF3I CTNAG 3 cut(s) 273, 372, 480
HpySE526I ACGT 4 cut(s) 16, 29, 590, 816
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 3 cut(s) 49, 1073, 1152
Kpn2I TCCGGA 1 cut(s) 1140
KpnI GGTACC 1 cut(s) 886
LmnI GCTCC 3 cut(s) 315, 417, 633
Lsp1109I GCAGC 2 cut(s) 832, 989
LweI GCATC 1 cut(s) 979
MaeI CTAG 2 cut(s) 1025, 1190
MaeII ACGT 4 cut(s) 16, 29, 590, 816
MaeIII GTNAC 2 cut(s) 725, 1178
MboII GAAGA 8 cut(s) 279, 372, 674, 706, 728, 806, 856, 895
MflI RGATCY 3 cut(s) 34, 269, 975
MhlI GDGCHC 3 cut(s) 320, 422, 638
MluCI AATT 6 cut(s) 95, 166, 348, 780, 1159, 1206
MlyI GAGTC 2 cut(s) 364, 614
MmeI TCCRAC 4 cut(s) 12, 209, 350, 1190
MroI TCCGGA 1 cut(s) 1140
MroXI GAANNNNTTC 2 cut(s) 247, 1200
MseI TTAA 4 cut(s) 737, 936, 1113, 1231
MslI CAYNNNNRTG 1 cut(s) 1074
MspI CCGG 7 cut(s) 194, 313, 466, 539, 602, 777, 1141
MspR9I CCNGG 3 cut(s) 825, 879, 1092
Mva1269I GAATGC 2 cut(s) 704, 1107
MvaI CCWGG 3 cut(s) 825, 879, 1092
MvnI CGCG 1 cut(s) 951
MwoI GCNNNNNNNGC 3 cut(s) 518, 633, 1117
NlaIII CATG 3 cut(s) 49, 1073, 1152
NlaIV GGNNCC 3 cut(s) 102, 473, 884
NmuCI GTSAC 2 cut(s) 725, 1178
NspI RCATGY 2 cut(s) 49, 1152
NspV TTCGAA 1 cut(s) 207
PciI ACATGT 1 cut(s) 1148
PctI GAATGC 2 cut(s) 704, 1107
PdmI GAANNNNTTC 2 cut(s) 247, 1200
PfeI GAWTC 2 cut(s) 54, 122
PflFI GACNNNGTC 1 cut(s) 899
PfoI TCCNGGA 1 cut(s) 1090
PinAI ACCGGT 2 cut(s) 193, 538
PkrI GCNGC 4 cut(s) 514, 822, 950, 1004
Ple19I CGATCG 1 cut(s) 690
PleI GAGTC 2 cut(s) 363, 613
PpsI GAGTC 2 cut(s) 363, 613
PscI ACATGT 1 cut(s) 1148
Psp6I CCWGG 3 cut(s) 823, 877, 1090
PspFI CCCAGC 1 cut(s) 889
PspGI CCWGG 3 cut(s) 823, 877, 1090
PspN4I GGNNCC 3 cut(s) 102, 473, 884
PspPI GGNCC 1 cut(s) 100
PsrI GAACNNNNNNTAC 2 cut(s) 832, 864
PsuI RGATCY 3 cut(s) 34, 269, 975
PsyI GACNNNGTC 1 cut(s) 899
PvuI CGATCG 1 cut(s) 690
RsaI GTAC 1 cut(s) 884
RsaNI GTAC 1 cut(s) 883
RseI CAYNNNNRTG 1 cut(s) 1074
SaqAI TTAA 4 cut(s) 737, 936, 1113, 1231
SatI GCNGC 4 cut(s) 513, 821, 949, 1003
Sau96I GGNCC 1 cut(s) 100
SchI GAGTC 2 cut(s) 364, 614
ScrFI CCNGG 3 cut(s) 825, 879, 1092
SduI GDGCHC 3 cut(s) 320, 422, 638
SfaNI GCATC 1 cut(s) 979
SfuI TTCGAA 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 1074
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
Sse9I AATT 6 cut(s) 95, 166, 348, 780, 1159, 1206
SsiI CCGC 4 cut(s) 238, 510, 513, 949
SspI AATATT 1 cut(s) 64
SspMI CTAG 2 cut(s) 1025, 1190
StyD4I CCNGG 3 cut(s) 823, 877, 1090
TaaI ACNGT 1 cut(s) 1173
TaiI ACGT 4 cut(s) 19, 32, 593, 819
TaqI TCGA 9 cut(s) 26, 59, 207, 344, 358, 398, 412, 658, 690
TaqII GACCGA 1 cut(s) 392
TasI AATT 6 cut(s) 95, 166, 348, 780, 1159, 1206
TauI GCSGC 2 cut(s) 515, 951
TfiI GAWTC 2 cut(s) 54, 122
Tru1I TTAA 4 cut(s) 737, 936, 1113, 1231
Tru9I TTAA 4 cut(s) 737, 936, 1113, 1231
TscAI CASTG 2 cut(s) 810, 999
TseFI GTSAC 2 cut(s) 725, 1178
TseI GCWGC 2 cut(s) 820, 1002
Tsp45I GTSAC 2 cut(s) 725, 1178
TspDTI ATGAA 4 cut(s) 107, 930, 1038, 1092
TspGWI ACGGA 2 cut(s) 149, 744
TspRI CASTG 2 cut(s) 810, 999
Tth111I GACNNNGTC 1 cut(s) 899
XagI CCTNNNNNAGG 1 cut(s) 1023
XapI RAATTY 3 cut(s) 166, 1159, 1206
XceI RCATGY 2 cut(s) 49, 1152
XmnI GAANNNNTTC 2 cut(s) 247, 1200
XspI CTAG 2 cut(s) 1025, 1190
ZraI GACGTC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.