RLG00000002125

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015884
Physical Location & Seq
Reverse (-)
25896314 .. 25897887
1574 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002125

Sequence Viewer

Length: 1251 bp
ATGCTCGGAATATCGAATATTTGCAAGGGATACAAATCCTTTTTATTGAAATGGAACACCCAAAACAAGCATTCTCGTTCGATTTCAGAAAGTGATGACACGTCCAAGGCGAAAGATGAGAATGATTTTATAATTAGTTATCCATCAACACCAATAGGTGGTTTGAATCATACGCACAGCATTGATGATAGCTTACTCATGCGTCGAACTCTTTCGAGGAATAAAAGTAGCAGAAGATGCAAGACCCCTACGCCAAGGTCCTTCTCAAGAAGTATGAGCCGGAGCACCACCCCGAATTCATTATCGAGCATTTCAAGTCGTAGGAACCCCTCCGAGACCGATATCCTTGCCTCTATAACAAGAAATCTGAGTCGGGAGGCCGAAATTGCTGCCTCTGTATCAAGGAATATAAAAAGGAATATGAGCAGCAATAAAAGCCGCAATTTGAGCAGGAATGCGAGCAGCAGACACCCGTCTGAGTCGGAATTACCCTCTCCATCACCTGCATCTCCACCGCCCGCCTCTCCAACACCGGCCTCACCTTCACCTGCCGCCTCTCCATCTACCGCTCGATCGCCTCATACCAGTAAAACAACAGGCACATCTGAAACGGACCCTACCACACCTCTATCAAGAACTCCGAGCAGGAGGAGCACCACCCCCATTGTGTTCTCTCAGACAACTGCTAGAAGGAAACCTCCCCCGGTTGAGAAGAAGCTTGAATTCACACTCGAGGAGTTGTGTCATGGATGCGTGAAGAAGATCCAGGTTACTAGAGATGTCATAAACAATGCCGGGATTATTGTGAAAGAAGAGGAAGTACTGAAAATAAACGTGCAGCCAGGTTGGAGGAAAGGAACCAAGATTACATTCGAAGGGAAAGGGGATGAGAAACCAGGGTACCTTCCAGCCGATATCATCTTCTTGATAGATGAAAAGAGGCATCATTTGTTCAAAAGAGCAGGCCGGGACGATTTGGAAATCGCTGTTGAGATTCCACTGGCAGATGCATTGGGAGGCTGCTCGTTTCCAGCTCCTTTGTTAGGAGGTGAAACAATGAGTCTGTCATTCGACGAAATTATACATCATGGTTATGAAAAGGTCATTGAAGGACAAGGAATGCCAAAGCTTAAAGAACCAACAAAGAGAGGTGACCTCCGCATTACTTGTCTCGTTGATTTTCCCAAAAAATTGAGCGACGAGCAACGAGCAGAAGCTGTTACAATTTTACAAGATTGTTCTTATCAGTAA

Protein Analysis

417

Amino Acids

45.84

Weight (kDa)

9.51

Isoelectric Point (pI)

72.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 237 - 395 3.4e-34 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11040
fragaria_vesca FvH4_5g29110
malus_domestica MD08G1235600.v1.1 MD15G1427300.v1.1
prunus_persica Prupe.1G571300_v2.0.a1
pyrus_communis pycom15g37880
rosa_chinensis RchiOBHm_Chr7g0221771
rosa_laevigata RLG00000002125
rosa_roxburghii Rroxscaffold_3G00237740
rosa_rugosa Rorug07G0203200
rosa_samantha Rh7AG344100 Rh7BG334800 Rh7CG361500 Rh7DG341900
rosa_wichuraiana Rw7G029180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AarI CACCTGC 2 cut(s) 511, 556
Acc36I ACCTGC 2 cut(s) 511, 556
Acc65I GGTACC 1 cut(s) 900
AccB1I GGYRCC 1 cut(s) 900
AccB7I CCANNNNNTGG 1 cut(s) 158
AccBSI CCGCTC 1 cut(s) 569
AciI CCGC 6 cut(s) 439, 515, 519, 552, 567, 1159
AclWI GGATC 1 cut(s) 757
AcsI RAATTY 2 cut(s) 295, 722
AfaI GTAC 2 cut(s) 822, 902
AfiI CCNNNNNNNGG 2 cut(s) 158, 1043
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 6 cut(s) 49, 166, 315, 722, 955, 1109
AjiI CACGTC 1 cut(s) 102
AjnI CCWGG 3 cut(s) 765, 841, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
AluBI AGCT 5 cut(s) 192, 718, 1034, 1129, 1217
AluI AGCT 5 cut(s) 192, 718, 1034, 1129, 1217
Alw21I GWGCWC 2 cut(s) 287, 656
Alw26I GTCTC 2 cut(s) 329, 1175
AlwI GGATC 1 cut(s) 757
AlwNI CAGNNNCTG 1 cut(s) 1217
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 3 cut(s) 378, 534, 964
ApeKI GCWGC 5 cut(s) 389, 426, 462, 838, 1020
ApoI RAATTY 2 cut(s) 295, 722
Asp700I GAANNNNTTC 1 cut(s) 211
Asp718I GGTACC 1 cut(s) 900
AspS9I GGNCC 2 cut(s) 258, 613
AsuC2I CCSGG 3 cut(s) 704, 796, 968
AsuHPI GGTGA 5 cut(s) 492, 531, 537, 1061, 1163
AsuII TTCGAA 1 cut(s) 873
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 2 cut(s) 258, 613
BaeI ACNNNNGTAYC 2 cut(s) 884, 917
BanI GGYRCC 1 cut(s) 900
BarI GAAGNNNNNNTAC 2 cut(s) 804, 836
Bbv12I GWGCWC 2 cut(s) 287, 656
BbvI GCAGC 5 cut(s) 376, 438, 474, 850, 1007
BccI CCATC 3 cut(s) 151, 505, 568
BcgI CGANNNNNNTGC 4 cut(s) 329, 363, 371, 405
BciT130I CCWGG 3 cut(s) 767, 843, 897
BciVI GTATCC 1 cut(s) 23
BcnI CCSGG 3 cut(s) 704, 796, 968
BcoDI GTCTC 2 cut(s) 329, 1175
BfaI CTAG 2 cut(s) 687, 774
BfuAI ACCTGC 2 cut(s) 511, 556
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 7 cut(s) 390, 427, 439, 463, 552, 839, 1021
BlsI GCNGC 7 cut(s) 391, 428, 440, 464, 553, 840, 1022
BmcAI AGTACT 1 cut(s) 822
Bme1390I CCNGG 6 cut(s) 704, 767, 796, 843, 897, 968
Bme18I GGWCC 2 cut(s) 258, 613
BmeT110I CYCGRG 1 cut(s) 731
BmgBI CACGTC 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 258, 613
BmiI GGNNCC 4 cut(s) 326, 615, 859, 902
BmrFI CCNGG 6 cut(s) 704, 767, 796, 843, 897, 968
BmsI GCATC 5 cut(s) 227, 515, 740, 952, 997
BoxI GACNNNNGTC 1 cut(s) 472
BplI GAGNNNNNCTC 2 cut(s) 1140, 1172
Bpu14I TTCGAA 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 250
BpuMI CCSGG 3 cut(s) 704, 796, 968
BsaBI GATNNNNATC 1 cut(s) 34
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 4 cut(s) 105, 254, 702, 896
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 1043
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 2 cut(s) 585, 1005
Bse8I GATNNNNATC 1 cut(s) 34
BseBI CCWGG 3 cut(s) 767, 843, 897
BseDI CCNNGG 4 cut(s) 105, 254, 702, 896
BseGI GGATG 2 cut(s) 755, 892
BseJI GATNNNNATC 1 cut(s) 34
BseLI CCNNNNNNNGG 2 cut(s) 158, 1043
BseMII CTCAG 3 cut(s) 359, 468, 689
BseNI ACTGG 2 cut(s) 585, 1005
BseRI GAGGAG 2 cut(s) 664, 749
BseXI GCAGC 5 cut(s) 376, 438, 474, 850, 1007
BsgI GTGCAG 1 cut(s) 857
Bsh1285I CGRYCG 1 cut(s) 575
BshFI GGCC 3 cut(s) 380, 536, 966
BshNI GGYRCC 1 cut(s) 900
BsiEI CGRYCG 1 cut(s) 575
BsiHKAI GWGCWC 2 cut(s) 287, 656
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 5 cut(s) 280, 533, 704, 795, 967
BslFI GGGAC 1 cut(s) 983
BslI CCNNNNNNNGG 2 cut(s) 158, 1043
BsmAI GTCTC 2 cut(s) 329, 1175
BsmFI GGGAC 1 cut(s) 983
BsmI GAATGC 3 cut(s) 70, 460, 1125
BsnI GGCC 3 cut(s) 380, 536, 966
Bso31I GGTCTC 1 cut(s) 329
BsoBI CYCGRG 1 cut(s) 731
Bsp119I TTCGAA 1 cut(s) 873
Bsp1286I GDGCHC 2 cut(s) 287, 656
Bsp143I GATC 2 cut(s) 572, 762
BspACI CCGC 6 cut(s) 439, 515, 519, 552, 567, 1159
BspANI GGCC 3 cut(s) 380, 536, 966
BspCNI CTCAG 3 cut(s) 360, 469, 688
BspLI GGNNCC 4 cut(s) 326, 615, 859, 902
BspMI ACCTGC 2 cut(s) 511, 556
BspPI GGATC 1 cut(s) 757
BspT104I TTCGAA 1 cut(s) 873
BspT107I GGYRCC 1 cut(s) 900
BspTNI GGTCTC 1 cut(s) 329
BsrBI CCGCTC 1 cut(s) 569
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 2 cut(s) 585, 1005
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 4 cut(s) 105, 254, 702, 896
BssMI GATC 2 cut(s) 572, 762
BssT1I CCWWGG 2 cut(s) 105, 254
Bst2UI CCWGG 3 cut(s) 767, 843, 897
Bst6I CTCTTC 1 cut(s) 807
BstAPI GCANNNNNTGC 1 cut(s) 237
BstBI TTCGAA 1 cut(s) 873
BstC8I GCNNGC 3 cut(s) 460, 519, 964
BstDEI CTNAG 3 cut(s) 368, 477, 675
BstEII GGTNACC 1 cut(s) 1151
BstENI CCTNNNNNAGG 1 cut(s) 1041
BstF5I GGATG 2 cut(s) 755, 892
BstKTI GATC 2 cut(s) 575, 765
BstMAI GTCTC 2 cut(s) 329, 1175
BstMBI GATC 2 cut(s) 572, 762
BstMCI CGRYCG 1 cut(s) 575
BstMWI GCNNNNNNNGC 5 cut(s) 237, 386, 435, 447, 651
BstNI CCWGG 3 cut(s) 767, 843, 897
BstPAI GACNNNNGTC 1 cut(s) 472
BstPI GGTNACC 1 cut(s) 1151
BstSCI CCNGG 6 cut(s) 702, 765, 794, 841, 895, 966
BstV1I GCAGC 5 cut(s) 376, 438, 474, 850, 1007
BstX2I RGATCY 1 cut(s) 762
BstYI RGATCY 1 cut(s) 762
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 3 cut(s) 380, 536, 966
BtrI CACGTC 1 cut(s) 102
BtsCI GGATG 2 cut(s) 755, 892
BtsIMutI CAGTG 1 cut(s) 998
BveI ACCTGC 2 cut(s) 511, 556
Cac8I GCNNGC 3 cut(s) 460, 519, 964
CaiI CAGNNNCTG 1 cut(s) 1217
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 2 cut(s) 258, 613
CseI GACGC 1 cut(s) 191
Csp6I GTAC 2 cut(s) 821, 901
CspCI CAANNNNNGTGG 2 cut(s) 646, 681
CviAII CATG 3 cut(s) 199, 746, 1088
CviQI GTAC 2 cut(s) 821, 901
DdeI CTNAG 3 cut(s) 368, 477, 675
DpnI GATC 2 cut(s) 574, 764
DpnII GATC 2 cut(s) 572, 762
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
Eco130I CCWWGG 2 cut(s) 105, 254
Eco31I GGTCTC 1 cut(s) 329
Eco32I GATATC 2 cut(s) 343, 916
Eco47I GGWCC 2 cut(s) 258, 613
Eco88I CYCGRG 1 cut(s) 731
Eco91I GGTNACC 1 cut(s) 1151
EcoNI CCTNNNNNAGG 1 cut(s) 1041
EcoO109I RGGNCCY 1 cut(s) 258
EcoO65I GGTNACC 1 cut(s) 1151
EcoRI GAATTC 2 cut(s) 295, 722
EcoRII CCWGG 3 cut(s) 765, 841, 895
EcoRV GATATC 2 cut(s) 343, 916
EcoT14I CCWWGG 2 cut(s) 105, 254
EcoT22I ATGCAT 1 cut(s) 1012
ErhI CCWWGG 2 cut(s) 105, 254
FaeI CATG 3 cut(s) 202, 749, 1091
FaqI GGGAC 1 cut(s) 983
FatI CATG 3 cut(s) 198, 745, 1087
FauI CCCGC 1 cut(s) 526
Fnu4HI GCNGC 7 cut(s) 390, 427, 439, 463, 552, 839, 1021
FokI GGATG 2 cut(s) 762, 899
Fsp4HI GCNGC 7 cut(s) 390, 427, 439, 463, 552, 839, 1021
FspBI CTAG 2 cut(s) 687, 774
GluI GCNGC 7 cut(s) 390, 427, 439, 463, 552, 839, 1021
HaeIII GGCC 3 cut(s) 380, 536, 966
HapII CCGG 5 cut(s) 280, 533, 704, 795, 967
HgaI GACGC 1 cut(s) 191
Hin1II CATG 3 cut(s) 202, 749, 1091
HindIII AAGCTT 2 cut(s) 716, 1127
HinfI GANTC 5 cut(s) 166, 370, 479, 994, 1060
HpaII CCGG 5 cut(s) 280, 533, 704, 795, 967
HphI GGTGA 5 cut(s) 492, 531, 537, 1061, 1163
Hpy188I TCNGA 9 cut(s) 8, 88, 334, 369, 478, 484, 607, 642, 678
Hpy188III TCNNGA 4 cut(s) 267, 374, 633, 925
Hpy99I CGWCG 3 cut(s) 207, 1076, 1202
HpyAV CCTTC 6 cut(s) 271, 552, 684, 869, 914, 1103
HpyCH4IV ACGT 2 cut(s) 101, 834
HpyCH4V TGCA 5 cut(s) 24, 240, 506, 838, 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 237, 386, 435, 447, 651
HpyF3I CTNAG 3 cut(s) 368, 477, 675
HpySE526I ACGT 2 cut(s) 101, 834
Hsp92II CATG 3 cut(s) 202, 749, 1091
KpnI GGTACC 1 cut(s) 904
Kzo9I GATC 2 cut(s) 572, 762
LmnI GCTCC 3 cut(s) 282, 651, 1039
Lsp1109I GCAGC 5 cut(s) 376, 438, 474, 850, 1007
LweI GCATC 5 cut(s) 227, 515, 740, 952, 997
MaeI CTAG 2 cut(s) 687, 774
MaeII ACGT 2 cut(s) 101, 834
MaeIII GTNAC 3 cut(s) 769, 1151, 1219
MalI GATC 2 cut(s) 574, 764
MbiI CCGCTC 1 cut(s) 569
MboI GATC 2 cut(s) 572, 762
MboII GAAGA 6 cut(s) 246, 724, 769, 772, 824, 913
MflI RGATCY 1 cut(s) 762
MhlI GDGCHC 2 cut(s) 287, 656
MluCI AATT 9 cut(s) 132, 295, 384, 442, 485, 722, 1077, 1190, 1224
MlyI GAGTC 3 cut(s) 379, 488, 1069
MmeI TCCRAC 3 cut(s) 462, 551, 827
Mph1103I ATGCAT 1 cut(s) 1012
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 1 cut(s) 1131
MslI CAYNNNNRTG 1 cut(s) 1092
MspI CCGG 5 cut(s) 280, 533, 704, 795, 967
MspR9I CCNGG 6 cut(s) 704, 767, 796, 843, 897, 968
Mva1269I GAATGC 3 cut(s) 70, 460, 1125
MvaI CCWGG 3 cut(s) 767, 843, 897
MwoI GCNNNNNNNGC 5 cut(s) 237, 386, 435, 447, 651
NciI CCSGG 3 cut(s) 704, 796, 968
NdeII GATC 2 cut(s) 572, 762
NlaIII CATG 3 cut(s) 202, 749, 1091
NlaIV GGNNCC 4 cut(s) 326, 615, 859, 902
NmuCI GTSAC 1 cut(s) 1151
NsiI ATGCAT 1 cut(s) 1012
NspV TTCGAA 1 cut(s) 873
PaeR7I CTCGAG 1 cut(s) 731
PaqCI CACCTGC 2 cut(s) 511, 556
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 3 cut(s) 70, 460, 1125
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 166, 994
PflMI CCANNNNNTGG 1 cut(s) 158
PkrI GCNGC 7 cut(s) 391, 428, 440, 464, 553, 840, 1022
Ple19I CGATCG 1 cut(s) 575
PleI GAGTC 3 cut(s) 378, 487, 1068
PpsI GAGTC 3 cut(s) 378, 487, 1068
PpuMI RGGWCCY 1 cut(s) 258
PshAI GACNNNNGTC 1 cut(s) 472
PsiI TTATAA 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 765, 841, 895
PspEI GGTNACC 1 cut(s) 1151
PspGI CCWGG 3 cut(s) 765, 841, 895
PspN4I GGNNCC 4 cut(s) 326, 615, 859, 902
PspPI GGNCC 2 cut(s) 258, 613
PspPPI RGGWCCY 1 cut(s) 258
PspXI VCTCGAGB 1 cut(s) 731
PsrI GAACNNNNNNTAC 2 cut(s) 850, 882
PstNI CAGNNNCTG 1 cut(s) 1217
PsuI RGATCY 1 cut(s) 762
PvuI CGATCG 1 cut(s) 575
RsaI GTAC 2 cut(s) 822, 902
RsaNI GTAC 2 cut(s) 821, 901
RseI CAYNNNNRTG 1 cut(s) 1092
SaqAI TTAA 1 cut(s) 1131
SatI GCNGC 7 cut(s) 390, 427, 439, 463, 552, 839, 1021
Sau3AI GATC 2 cut(s) 572, 762
Sau96I GGNCC 2 cut(s) 258, 613
ScaI AGTACT 1 cut(s) 822
SchI GAGTC 3 cut(s) 379, 488, 1069
ScrFI CCNGG 6 cut(s) 704, 767, 796, 843, 897, 968
SduI GDGCHC 2 cut(s) 287, 656
SfaNI GCATC 5 cut(s) 227, 515, 740, 952, 997
Sfr274I CTCGAG 1 cut(s) 731
SfuI TTCGAA 1 cut(s) 873
SinI GGWCC 2 cut(s) 258, 613
SlaI CTCGAG 1 cut(s) 731
SmiMI CAYNNNNRTG 1 cut(s) 1092
SmlI CTYRAG 2 cut(s) 265, 731
SmoI CTYRAG 2 cut(s) 265, 731
Sse9I AATT 9 cut(s) 132, 295, 384, 442, 485, 722, 1077, 1190, 1224
SsiI CCGC 6 cut(s) 439, 515, 519, 552, 567, 1159
SspI AATATT 1 cut(s) 19
SspMI CTAG 2 cut(s) 687, 774
StyD4I CCNGG 6 cut(s) 702, 765, 794, 841, 895, 966
StyI CCWWGG 2 cut(s) 105, 254
TaiI ACGT 2 cut(s) 104, 837
TaqI TCGA 9 cut(s) 14, 80, 205, 215, 305, 571, 732, 873, 1071
TaqII GACCGA 1 cut(s) 353
TasI AATT 9 cut(s) 132, 295, 384, 442, 485, 722, 1077, 1190, 1224
TatI WGTACW 1 cut(s) 820
TauI GCSGC 2 cut(s) 441, 554
TfiI GAWTC 2 cut(s) 166, 994
Tru1I TTAA 1 cut(s) 1131
Tru9I TTAA 1 cut(s) 1131
TscAI CASTG 1 cut(s) 1005
TseFI GTSAC 1 cut(s) 1151
TseI GCWGC 5 cut(s) 389, 426, 462, 838, 1020
Tsp45I GTSAC 1 cut(s) 1151
TspDTI ATGAA 3 cut(s) 288, 948, 1110
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 1 cut(s) 1005
Van91I CCANNNNNTGG 1 cut(s) 158
VpaK11BI GGWCC 2 cut(s) 258, 613
XagI CCTNNNNNAGG 1 cut(s) 1041
XapI RAATTY 2 cut(s) 295, 722
XhoI CTCGAG 1 cut(s) 731
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 2 cut(s) 687, 774
ZrmI AGTACT 1 cut(s) 822
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.