Rw7G029180

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015884
Physical Location & Seq
Forward (+)
40319676 .. 40321246
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G029180.1

Sequence Viewer

Length: 1248 bp
ATGCTCGGAATATCGAATATTTGCAAGGGATACAAATCCTTTTTATTGAAATGGAACGCCCAAAACAAGCATTCTCGTTCGATTTCAGAAAGTGATGACACGTCCAAGAAAGATGCGAATGATTTTATAATTAGTTATCCATCAACACCAGTAGGTGGTTTGAATCATACGCACAGCATTGATGATAGCTTACTCATGCGTCGAACTCTTTCGAGGAATAAAAGTAGCAGAAGATGCAAGACCCCTACGCCAAGGTCCTTCTCAAGAAGTATGAGCAGGAGCACCACCCCGAATTCATTATCGAGCATTTCAAGTCGTAGGAACCCCTCCGAGACCGATATCCTTGCCTCTATAACAAGAAATCTGAGTCGGGAGGCCGAAATTGCTGCCTCTATATCAAGGAATATAAGTAGGAATATGAGCAGCAATAAAAGCCGCAATTTGAGCAGGAATGCGAGCAGCAGACACCCGTCTGAGTCGGAATTACCCTCTCCATCACCTGCATCTCCACCGCCCGCCACTCCAACACCGGCCTCACCTTCACCTGCCGCCTCTCCATCTACCGCTCGATCGCCTCATACCAGTAAAACAACAGGCACATCTGAAACGGACCCTACCACACCTCTATCAAGAACTCCGAGCAGGAGGAGCACCACCCCCATTGTGTTCTCTCAGACAACTGCTAGAAGGAAACCTCCCCCGGTTGAGAAGAAGCTTGAATTCACACTCGAGGAGCTGTGTCATGGATGCGTGAAGAAGATCCAGGTTACTAGAGATGTCATAAACAATGCCGGGATTATTGTGAAAGAAGAGGAAGTACTGAAAATAAACGTGCAGCCAGGTTGGAGGAAAGGAACCAAGATTACATTCGAAGGGAAAGGGGATGAGAAACCAGGGTACCTTCCAGCCGATATCATCTTCTTGATAGATGAAAAGAGGCATCCTTTGTTCAAAAGAGCAGGCCGGGACGATTTGGAAATCGCTGTTGAGATTCCACTGGCGGATGCATTGGGAGGCTGCTCGTTTCCAGCTCCTTTGTTAGGAGGTGAAACAATGAGTCTGTCATTCGACGAAATTATACATCATGGTTATGAAAAGGTCATTGAAGGACAAGGAATGCCAAAGCTTAAAGAACCAACAAAGAGAGGTGACCTCCGCATTACTTGTCTCGTTAATTTTCCCAAAAAATTGAGCGACGAGCAACGAGCAGAAGCTGTTTCAATTTTACAAGATTGTTCTTATCAGTAA

Protein Analysis

415

Amino Acids

45.6

Weight (kDa)

9.58

Isoelectric Point (pI)

71.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 236 - 394 9.8e-35 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11040
fragaria_vesca FvH4_5g29110
malus_domestica MD08G1235600.v1.1 MD15G1427300.v1.1
prunus_persica Prupe.1G571300_v2.0.a1
pyrus_communis pycom15g37880
rosa_chinensis RchiOBHm_Chr7g0221771
rosa_laevigata RLG00000002125
rosa_roxburghii Rroxscaffold_3G00237740
rosa_rugosa Rorug07G0203200
rosa_samantha Rh7AG344100 Rh7BG334800 Rh7CG361500 Rh7DG341900
rosa_wichuraiana Rw7G029180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
AarI CACCTGC 2 cut(s) 508, 553
Acc36I ACCTGC 2 cut(s) 508, 553
Acc65I GGTACC 1 cut(s) 897
AccB1I GGYRCC 1 cut(s) 897
AccB7I CCANNNNNTGG 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 566
AciI CCGC 7 cut(s) 436, 512, 516, 549, 564, 1001, 1156
AclWI GGATC 1 cut(s) 754
AcsI RAATTY 2 cut(s) 292, 719
AfaI GTAC 2 cut(s) 819, 899
AfiI CCNNNNNNNGG 2 cut(s) 155, 1040
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 7 cut(s) 49, 163, 312, 719, 952, 1106, 1221
AjiI CACGTC 1 cut(s) 102
AjnI CCWGG 3 cut(s) 762, 838, 892
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
AluBI AGCT 6 cut(s) 189, 715, 736, 1031, 1126, 1214
AluI AGCT 6 cut(s) 189, 715, 736, 1031, 1126, 1214
Alw21I GWGCWC 2 cut(s) 284, 653
Alw26I GTCTC 2 cut(s) 326, 1172
AlwI GGATC 1 cut(s) 754
AlwNI CAGNNNCTG 1 cut(s) 1214
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 3 cut(s) 375, 531, 961
ApeKI GCWGC 5 cut(s) 386, 423, 459, 835, 1017
ApoI RAATTY 2 cut(s) 292, 719
Asp700I GAANNNNTTC 1 cut(s) 208
Asp718I GGTACC 1 cut(s) 897
AspS9I GGNCC 2 cut(s) 255, 610
AsuC2I CCSGG 3 cut(s) 701, 793, 965
AsuHPI GGTGA 5 cut(s) 489, 528, 534, 1058, 1160
AsuII TTCGAA 1 cut(s) 870
AvaI CYCGRG 1 cut(s) 728
AvaII GGWCC 2 cut(s) 255, 610
BaeI ACNNNNGTAYC 2 cut(s) 881, 914
BanI GGYRCC 1 cut(s) 897
BarI GAAGNNNNNNTAC 2 cut(s) 801, 833
Bbv12I GWGCWC 2 cut(s) 284, 653
BbvI GCAGC 5 cut(s) 373, 435, 471, 847, 1004
BccI CCATC 3 cut(s) 148, 502, 565
BcgI CGANNNNNNTGC 4 cut(s) 326, 360, 368, 402
BciT130I CCWGG 3 cut(s) 764, 840, 894
BciVI GTATCC 1 cut(s) 23
BcnI CCSGG 3 cut(s) 701, 793, 965
BcoDI GTCTC 2 cut(s) 326, 1172
BfaI CTAG 2 cut(s) 684, 771
BfuAI ACCTGC 2 cut(s) 508, 553
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 7 cut(s) 387, 424, 436, 460, 549, 836, 1018
BlsI GCNGC 7 cut(s) 388, 425, 437, 461, 550, 837, 1019
BmcAI AGTACT 1 cut(s) 819
Bme1390I CCNGG 6 cut(s) 701, 764, 793, 840, 894, 965
Bme18I GGWCC 2 cut(s) 255, 610
BmeT110I CYCGRG 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 255, 610
BmiI GGNNCC 4 cut(s) 323, 612, 856, 899
BmrFI CCNGG 6 cut(s) 701, 764, 793, 840, 894, 965
BmsI GCATC 6 cut(s) 103, 224, 512, 737, 949, 994
BoxI GACNNNNGTC 1 cut(s) 469
BplI GAGNNNNNCTC 2 cut(s) 1137, 1169
Bpu14I TTCGAA 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 247
BpuMI CCSGG 3 cut(s) 701, 793, 965
BsaBI GATNNNNATC 1 cut(s) 34
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 251, 699, 893
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 1040
Bse118I RCCGGY 1 cut(s) 529
Bse1I ACTGG 3 cut(s) 149, 582, 1002
Bse8I GATNNNNATC 1 cut(s) 34
BseBI CCWGG 3 cut(s) 764, 840, 894
BseDI CCNNGG 3 cut(s) 251, 699, 893
BseGI GGATG 4 cut(s) 752, 889, 940, 1009
BseJI GATNNNNATC 1 cut(s) 34
BseLI CCNNNNNNNGG 2 cut(s) 155, 1040
BseMII CTCAG 3 cut(s) 356, 465, 686
BseNI ACTGG 3 cut(s) 149, 582, 1002
BseRI GAGGAG 2 cut(s) 661, 746
BseXI GCAGC 5 cut(s) 373, 435, 471, 847, 1004
BsgI GTGCAG 1 cut(s) 854
Bsh1285I CGRYCG 1 cut(s) 572
BshFI GGCC 3 cut(s) 377, 533, 963
BshNI GGYRCC 1 cut(s) 897
BsiEI CGRYCG 1 cut(s) 572
BsiHKAI GWGCWC 2 cut(s) 284, 653
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 4 cut(s) 530, 701, 792, 964
BslFI GGGAC 1 cut(s) 980
BslI CCNNNNNNNGG 2 cut(s) 155, 1040
BsmAI GTCTC 2 cut(s) 326, 1172
BsmFI GGGAC 1 cut(s) 980
BsmI GAATGC 3 cut(s) 70, 457, 1122
BsnI GGCC 3 cut(s) 377, 533, 963
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 728
Bsp119I TTCGAA 1 cut(s) 870
Bsp1286I GDGCHC 2 cut(s) 284, 653
Bsp143I GATC 2 cut(s) 569, 759
BspACI CCGC 7 cut(s) 436, 512, 516, 549, 564, 1001, 1156
BspANI GGCC 3 cut(s) 377, 533, 963
BspCNI CTCAG 3 cut(s) 357, 466, 685
BspLI GGNNCC 4 cut(s) 323, 612, 856, 899
BspMI ACCTGC 2 cut(s) 508, 553
BspPI GGATC 1 cut(s) 754
BspT104I TTCGAA 1 cut(s) 870
BspT107I GGYRCC 1 cut(s) 897
BspTNI GGTCTC 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 566
BsrFI RCCGGY 1 cut(s) 529
BsrI ACTGG 3 cut(s) 149, 582, 1002
BssAI RCCGGY 1 cut(s) 529
BssECI CCNNGG 3 cut(s) 251, 699, 893
BssMI GATC 2 cut(s) 569, 759
BssT1I CCWWGG 1 cut(s) 251
Bst2UI CCWGG 3 cut(s) 764, 840, 894
Bst6I CTCTTC 1 cut(s) 804
BstAPI GCANNNNNTGC 1 cut(s) 234
BstBI TTCGAA 1 cut(s) 870
BstC8I GCNNGC 3 cut(s) 457, 516, 961
BstDEI CTNAG 3 cut(s) 365, 474, 672
BstEII GGTNACC 1 cut(s) 1148
BstENI CCTNNNNNAGG 1 cut(s) 1038
BstF5I GGATG 4 cut(s) 752, 889, 940, 1009
BstKTI GATC 2 cut(s) 572, 762
BstMAI GTCTC 2 cut(s) 326, 1172
BstMBI GATC 2 cut(s) 569, 759
BstMCI CGRYCG 1 cut(s) 572
BstMWI GCNNNNNNNGC 5 cut(s) 234, 383, 432, 444, 648
BstNI CCWGG 3 cut(s) 764, 840, 894
BstPAI GACNNNNGTC 1 cut(s) 469
BstPI GGTNACC 1 cut(s) 1148
BstSCI CCNGG 6 cut(s) 699, 762, 791, 838, 892, 963
BstV1I GCAGC 5 cut(s) 373, 435, 471, 847, 1004
BstX2I RGATCY 1 cut(s) 759
BstYI RGATCY 1 cut(s) 759
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 3 cut(s) 377, 533, 963
BtrI CACGTC 1 cut(s) 102
BtsCI GGATG 4 cut(s) 752, 889, 940, 1009
BtsIMutI CAGTG 1 cut(s) 995
BveI ACCTGC 2 cut(s) 508, 553
Cac8I GCNNGC 3 cut(s) 457, 516, 961
CaiI CAGNNNCTG 1 cut(s) 1214
Cfr10I RCCGGY 1 cut(s) 529
Cfr13I GGNCC 2 cut(s) 255, 610
CseI GACGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 818, 898
CspCI CAANNNNNGTGG 2 cut(s) 643, 678
CviAII CATG 3 cut(s) 196, 743, 1085
CviQI GTAC 2 cut(s) 818, 898
DdeI CTNAG 3 cut(s) 365, 474, 672
DpnI GATC 2 cut(s) 571, 761
DpnII GATC 2 cut(s) 569, 759
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
EciI GGCGGA 1 cut(s) 1016
Eco130I CCWWGG 1 cut(s) 251
Eco31I GGTCTC 1 cut(s) 326
Eco32I GATATC 2 cut(s) 340, 913
Eco47I GGWCC 2 cut(s) 255, 610
Eco88I CYCGRG 1 cut(s) 728
Eco91I GGTNACC 1 cut(s) 1148
EcoNI CCTNNNNNAGG 1 cut(s) 1038
EcoO109I RGGNCCY 1 cut(s) 255
EcoO65I GGTNACC 1 cut(s) 1148
EcoRI GAATTC 2 cut(s) 292, 719
EcoRII CCWGG 3 cut(s) 762, 838, 892
EcoRV GATATC 2 cut(s) 340, 913
EcoT14I CCWWGG 1 cut(s) 251
EcoT22I ATGCAT 1 cut(s) 1009
ErhI CCWWGG 1 cut(s) 251
FaeI CATG 3 cut(s) 199, 746, 1088
FaqI GGGAC 1 cut(s) 980
FatI CATG 3 cut(s) 195, 742, 1084
FauI CCCGC 1 cut(s) 523
Fnu4HI GCNGC 7 cut(s) 387, 424, 436, 460, 549, 836, 1018
FokI GGATG 4 cut(s) 759, 896, 927, 1016
Fsp4HI GCNGC 7 cut(s) 387, 424, 436, 460, 549, 836, 1018
FspBI CTAG 2 cut(s) 684, 771
GluI GCNGC 7 cut(s) 387, 424, 436, 460, 549, 836, 1018
HaeIII GGCC 3 cut(s) 377, 533, 963
HapII CCGG 4 cut(s) 530, 701, 792, 964
HgaI GACGC 1 cut(s) 188
Hin1II CATG 3 cut(s) 199, 746, 1088
HindIII AAGCTT 2 cut(s) 713, 1124
HinfI GANTC 5 cut(s) 163, 367, 476, 991, 1057
HpaII CCGG 4 cut(s) 530, 701, 792, 964
HphI GGTGA 5 cut(s) 489, 528, 534, 1058, 1160
Hpy188I TCNGA 9 cut(s) 8, 88, 331, 366, 475, 481, 604, 639, 675
Hpy188III TCNNGA 4 cut(s) 264, 371, 630, 922
Hpy99I CGWCG 3 cut(s) 204, 1073, 1199
HpyAV CCTTC 6 cut(s) 268, 549, 681, 866, 911, 1100
HpyCH4IV ACGT 2 cut(s) 101, 831
HpyCH4V TGCA 5 cut(s) 24, 237, 503, 835, 1007
HpyF10VI GCNNNNNNNGC 5 cut(s) 234, 383, 432, 444, 648
HpyF3I CTNAG 3 cut(s) 365, 474, 672
HpySE526I ACGT 2 cut(s) 101, 831
Hsp92II CATG 3 cut(s) 199, 746, 1088
KpnI GGTACC 1 cut(s) 901
Kzo9I GATC 2 cut(s) 569, 759
LmnI GCTCC 4 cut(s) 279, 648, 733, 1036
Lsp1109I GCAGC 5 cut(s) 373, 435, 471, 847, 1004
LweI GCATC 6 cut(s) 103, 224, 512, 737, 949, 994
MaeI CTAG 2 cut(s) 684, 771
MaeII ACGT 2 cut(s) 101, 831
MaeIII GTNAC 2 cut(s) 766, 1148
MalI GATC 2 cut(s) 571, 761
MbiI CCGCTC 1 cut(s) 566
MboI GATC 2 cut(s) 569, 759
MboII GAAGA 6 cut(s) 243, 721, 766, 769, 821, 910
MflI RGATCY 1 cut(s) 759
MhlI GDGCHC 2 cut(s) 284, 653
MlyI GAGTC 3 cut(s) 376, 485, 1066
MmeI TCCRAC 3 cut(s) 459, 548, 824
Mph1103I ATGCAT 1 cut(s) 1009
MroXI GAANNNNTTC 1 cut(s) 208
MseI TTAA 2 cut(s) 1128, 1173
MslI CAYNNNNRTG 1 cut(s) 1089
MspI CCGG 4 cut(s) 530, 701, 792, 964
MspR9I CCNGG 6 cut(s) 701, 764, 793, 840, 894, 965
Mva1269I GAATGC 3 cut(s) 70, 457, 1122
MvaI CCWGG 3 cut(s) 764, 840, 894
MwoI GCNNNNNNNGC 5 cut(s) 234, 383, 432, 444, 648
NciI CCSGG 3 cut(s) 701, 793, 965
NdeII GATC 2 cut(s) 569, 759
NlaIII CATG 3 cut(s) 199, 746, 1088
NlaIV GGNNCC 4 cut(s) 323, 612, 856, 899
NmuCI GTSAC 1 cut(s) 1148
NsiI ATGCAT 1 cut(s) 1009
NspV TTCGAA 1 cut(s) 870
PaeR7I CTCGAG 1 cut(s) 728
PaqCI CACCTGC 2 cut(s) 508, 553
PctI GAATGC 3 cut(s) 70, 457, 1122
PdmI GAANNNNTTC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 163, 991
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 7 cut(s) 388, 425, 437, 461, 550, 837, 1019
Ple19I CGATCG 1 cut(s) 572
PleI GAGTC 3 cut(s) 375, 484, 1065
PpsI GAGTC 3 cut(s) 375, 484, 1065
PpuMI RGGWCCY 1 cut(s) 255
PshAI GACNNNNGTC 1 cut(s) 469
PsiI TTATAA 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 255
Psp6I CCWGG 3 cut(s) 762, 838, 892
PspEI GGTNACC 1 cut(s) 1148
PspGI CCWGG 3 cut(s) 762, 838, 892
PspN4I GGNNCC 4 cut(s) 323, 612, 856, 899
PspPI GGNCC 2 cut(s) 255, 610
PspPPI RGGWCCY 1 cut(s) 255
PspXI VCTCGAGB 1 cut(s) 728
PsrI GAACNNNNNNTAC 2 cut(s) 847, 879
PstNI CAGNNNCTG 1 cut(s) 1214
PsuI RGATCY 1 cut(s) 759
PvuI CGATCG 1 cut(s) 572
RsaI GTAC 2 cut(s) 819, 899
RsaNI GTAC 2 cut(s) 818, 898
RseI CAYNNNNRTG 1 cut(s) 1089
SaqAI TTAA 2 cut(s) 1128, 1173
SatI GCNGC 7 cut(s) 387, 424, 436, 460, 549, 836, 1018
Sau3AI GATC 2 cut(s) 569, 759
Sau96I GGNCC 2 cut(s) 255, 610
ScaI AGTACT 1 cut(s) 819
SchI GAGTC 3 cut(s) 376, 485, 1066
ScrFI CCNGG 6 cut(s) 701, 764, 793, 840, 894, 965
SduI GDGCHC 2 cut(s) 284, 653
SfaNI GCATC 6 cut(s) 103, 224, 512, 737, 949, 994
Sfr274I CTCGAG 1 cut(s) 728
SfuI TTCGAA 1 cut(s) 870
SinI GGWCC 2 cut(s) 255, 610
SlaI CTCGAG 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 1089
SmlI CTYRAG 2 cut(s) 262, 728
SmoI CTYRAG 2 cut(s) 262, 728
SsiI CCGC 7 cut(s) 436, 512, 516, 549, 564, 1001, 1156
SspI AATATT 1 cut(s) 19
SspMI CTAG 2 cut(s) 684, 771
StyD4I CCNGG 6 cut(s) 699, 762, 791, 838, 892, 963
StyI CCWWGG 1 cut(s) 251
TaiI ACGT 2 cut(s) 104, 834
TaqI TCGA 9 cut(s) 14, 80, 202, 212, 302, 568, 729, 870, 1068
TaqII GACCGA 1 cut(s) 350
TatI WGTACW 1 cut(s) 817
TauI GCSGC 2 cut(s) 438, 551
TfiI GAWTC 2 cut(s) 163, 991
Tru1I TTAA 2 cut(s) 1128, 1173
Tru9I TTAA 2 cut(s) 1128, 1173
TscAI CASTG 1 cut(s) 1002
TseFI GTSAC 1 cut(s) 1148
TseI GCWGC 5 cut(s) 386, 423, 459, 835, 1017
Tsp45I GTSAC 1 cut(s) 1148
TspDTI ATGAA 3 cut(s) 285, 945, 1107
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 1 cut(s) 1002
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 2 cut(s) 255, 610
XagI CCTNNNNNAGG 1 cut(s) 1038
XapI RAATTY 2 cut(s) 292, 719
XhoI CTCGAG 1 cut(s) 728
XmnI GAANNNNTTC 1 cut(s) 208
XspI CTAG 2 cut(s) 684, 771
ZrmI AGTACT 1 cut(s) 819
Zsp2I ATGCAT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.