FvH4_5g29340

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006792
Physical Location & Seq
Reverse (-)
20326385 .. 20335417
9033 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29340.t3

Sequence Viewer

Length: 1125 bp
ATGGAGGTGTCGGCACTCTGCCGAAACTCTGTTCCATTTGTTCTCCCAGTGCCACCTTGGAGCACAAGAGTGAAGACGTCTCAGGTCTCTTGTGCCACAACTCTTGACAACCAGCCCCTCCGGCATGACAGCAGGAGGGTCACAAGGCCTCACTCCAAGCAATATCTAGCTAGACAATCTGCAATTCTTCAAGTCCAGCACTCCTCTGACTTAGAATCAGCTCTTACAAGATTAGGTGGAAGTTTGAACGTTCAAGACTTGAATGCCATAATACGTCATTTTGGGATGCTGAAAAGATGGCATGATCTTTCCCAGCTCTTTGAATGGATGCAACAGAATGGAAAAGTTAGTGCATCGTCCTATAGTAGCTATATAAAATTCATGGGAAAGAGCCTCAATCCCGTAAAAGCACTAGAAATTTACAATAGCATTCAAGATGAATCAACTAAAAAGAATGTTCACATATGCAATTCGGTTCTTGGTAGTCTGGTCAGGAGTGGCAAGTTTGATGGCAGTATTAAGTTATTTCACCAGATGAAGCAGGATGGCTTAACACCAGATGCTGTCACCTATAGTACGCTTCTTGCAGGTTGCATCAAATTCAAACATGGTTATTCTAAGGCATTAGAGCTGGTTCAAGAATTACAGAATAATGAGCTGCAGATGGACAGTGTAATATATGGTACACTTTTGGCTATCTGTGCTTCAAATAATAAATGGGAAGAAGCAGAAAGTTATTTCAAGCAAATGAAGGATGAAGGCCATTTACCAAACGAATTCCATTACAGCTCTTTGCTCAATGCTTACTCTATAAGTGGGAACTACAAAAAGGCTGATGATGTGGTGCAAGATATGAAATCTGCAGGGTTAGTACCTAATAAGGTTACTTTGACAACTTTATTGAAGGCTTATGTCAGAGGTGGGTTGTTTGAAAAATCAAGAGAATTACTAACCGAACTGGAAGCTTTGGGATATGCTGAAGATGAGATGCCATACTGTATATTGATGGATGCCTTTGCTAAGGCTGGCCGTATAGAAGATGCTAAATTGGTTTTTGATGAAATAAAGGAGAAATCTGTACGATCAGGTATCTTTCAAATATTACCATATTCAAACCCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

375

Amino Acids

42.08

Weight (kDa)

8.36

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 151 - 200 7.3e-13 PPR repeat family
PPR_long PF17177 205 - 345 6.1e-14 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 211 - 269 1.9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 223 - 269 2.3e-09 PPR repeat family
PPR PF01535 225 - 254 9.1e-06 PPR repeat
TPR_24 PF23276 231 - 347 8.9e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 257 - 305 3.9e-10 PPR repeat family
PPR_3 PF13812 260 - 305 4.7e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 332 - 376 2.6e-10 PPR repeat family
PPR_1 PF12854 363 - 386 7.7e-06 PPR repeat
PPR_2 PF13041 398 - 446 2e-11 PPR repeat family
PPR PF01535 400 - 428 5.7e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 578
AclI AACGTT 1 cut(s) 249
AcoI YGGCCR 1 cut(s) 1027
AcsI RAATTY 4 cut(s) 377, 417, 599, 776
AcuI CTGAAG 1 cut(s) 999
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 577, 685, 873, 1080
AleI CACNNNNGTG 1 cut(s) 68
AluBI AGCT 8 cut(s) 170, 221, 316, 369, 631, 658, 789, 965
AluI AGCT 8 cut(s) 170, 221, 316, 369, 631, 658, 789, 965
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 2 cut(s) 84, 91
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 3 cut(s) 146, 760, 1027
ApeKI GCWGC 1 cut(s) 658
ApoI RAATTY 4 cut(s) 377, 417, 599, 776
AsuHPI GGTGA 2 cut(s) 521, 559
BarI GAAGNNNNNNTAC 2 cut(s) 750, 782
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 1 cut(s) 645
BccI CCATC 5 cut(s) 291, 503, 539, 658, 1000
BceAI ACGGC 1 cut(s) 1014
BcoDI GTCTC 2 cut(s) 84, 91
BfaI CTAG 3 cut(s) 167, 171, 413
BfmI CTRYAG 4 cut(s) 361, 571, 659, 861
BfuAI ACCTGC 1 cut(s) 578
BglI GCCNNNNNGGC 1 cut(s) 121
BisI GCNGC 1 cut(s) 659
BlsI GCNGC 1 cut(s) 660
BmrI ACTGGG 1 cut(s) 41
BmsI GCATC 8 cut(s) 276, 318, 362, 550, 603, 978, 1000, 1030
BmuI ACTGGG 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 80
Bpu10I CCTNAGC 1 cut(s) 1020
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 1 cut(s) 56
Bse1I ACTGG 2 cut(s) 47, 963
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 5 cut(s) 291, 333, 550, 760, 1015
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 2 cut(s) 47, 963
BseRI GAGGAG 1 cut(s) 193
BseXI GCAGC 1 cut(s) 645
BseYI CCCAGC 1 cut(s) 312
BshFI GGCC 3 cut(s) 148, 762, 1029
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 1 cut(s) 121
BsmAI GTCTC 2 cut(s) 84, 91
BsmBI CGTCTC 1 cut(s) 84
BsmI GAATGC 2 cut(s) 268, 429
BsnI GGCC 3 cut(s) 148, 762, 1029
Bso31I GGTCTC 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 2 cut(s) 304, 1082
BspANI GGCC 3 cut(s) 148, 762, 1029
BspCNI CTCAG 1 cut(s) 94
BspMAI CTGCAG 2 cut(s) 663, 865
BspMI ACCTGC 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 91
BsrI ACTGG 2 cut(s) 47, 963
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 2 cut(s) 304, 1082
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 56
Bst4CI ACNGT 2 cut(s) 671, 998
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 1027
BstDEI CTNAG 5 cut(s) 81, 211, 618, 1020, 1119
BstF5I GGATG 5 cut(s) 291, 333, 550, 760, 1015
BstKTI GATC 2 cut(s) 307, 1085
BstMAI GTCTC 2 cut(s) 84, 91
BstMBI GATC 2 cut(s) 304, 1082
BstMWI GCNNNNNNNGC 2 cut(s) 121, 701
BstSFI CTRYAG 4 cut(s) 361, 571, 659, 861
BstV1I GCAGC 1 cut(s) 645
BstV2I GAAGAC 1 cut(s) 80
BsuRI GGCC 3 cut(s) 148, 762, 1029
BtsCI GGATG 5 cut(s) 291, 333, 550, 760, 1015
BtsIMutI CAGTG 2 cut(s) 54, 676
BveI ACCTGC 1 cut(s) 578
Cac8I GCNNGC 1 cut(s) 1027
CaiI CAGNNNCTG 1 cut(s) 563
Csp6I GTAC 4 cut(s) 576, 684, 872, 1079
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviAII CATG 4 cut(s) 125, 302, 382, 608
CviQI GTAC 4 cut(s) 576, 684, 872, 1079
DdeI CTNAG 5 cut(s) 81, 211, 618, 1020, 1119
DpnI GATC 2 cut(s) 306, 1084
DpnII GATC 2 cut(s) 304, 1082
EaeI YGGCCR 1 cut(s) 1027
Eco130I CCWWGG 1 cut(s) 56
Eco147I AGGCCT 1 cut(s) 148
Eco31I GGTCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 999
EcoRI GAATTC 1 cut(s) 776
EcoT14I CCWWGG 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 56
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 4 cut(s) 128, 305, 385, 611
FatI CATG 4 cut(s) 124, 301, 381, 607
FauNDI CATATG 1 cut(s) 464
Fnu4HI GCNGC 1 cut(s) 659
FokI GGATG 5 cut(s) 298, 340, 557, 767, 1022
Fsp4HI GCNGC 1 cut(s) 659
FspBI CTAG 3 cut(s) 167, 171, 413
GluI GCNGC 1 cut(s) 659
GsaI CCCAGC 1 cut(s) 316
HaeIII GGCC 3 cut(s) 148, 762, 1029
HapII CCGG 1 cut(s) 121
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 4 cut(s) 128, 305, 385, 611
HindIII AAGCTT 1 cut(s) 963
HinfI GANTC 2 cut(s) 215, 440
HpaII CCGG 1 cut(s) 121
HphI GGTGA 2 cut(s) 521, 559
Hpy166II GTNNAC 2 cut(s) 460, 686
Hpy188I TCNGA 2 cut(s) 208, 917
Hpy188III TCNNGA 6 cut(s) 104, 254, 434, 493, 638, 939
Hpy8I GTNNAC 2 cut(s) 460, 686
HpyAV CCTTC 3 cut(s) 745, 752, 898
HpyCH4III ACNGT 2 cut(s) 671, 998
HpyCH4IV ACGT 3 cut(s) 77, 249, 274
HpyCH4V TGCA 9 cut(s) 182, 331, 353, 468, 587, 594, 661, 847, 863
HpyF10VI GCNNNNNNNGC 2 cut(s) 121, 701
HpyF3I CTNAG 5 cut(s) 81, 211, 618, 1020, 1119
HpySE526I ACGT 3 cut(s) 77, 249, 274
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 4 cut(s) 128, 305, 385, 611
Kzo9I GATC 2 cut(s) 304, 1082
LmnI GCTCC 1 cut(s) 60
Lsp1109I GCAGC 1 cut(s) 645
LweI GCATC 8 cut(s) 276, 318, 362, 550, 603, 978, 1000, 1030
MaeI CTAG 3 cut(s) 167, 171, 413
MaeII ACGT 3 cut(s) 77, 249, 274
MaeIII GTNAC 3 cut(s) 139, 565, 883
MalI GATC 2 cut(s) 306, 1084
MboI GATC 2 cut(s) 304, 1082
MboII GAAGA 5 cut(s) 85, 179, 734, 992, 1049
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 9 cut(s) 183, 377, 417, 469, 599, 641, 776, 944, 1046
MnlI CCTC 6 cut(s) 128, 129, 159, 214, 404, 911
MseI TTAA 2 cut(s) 519, 551
MslI CAYNNNNRTG 1 cut(s) 68
MspI CCGG 1 cut(s) 121
Mva1269I GAATGC 2 cut(s) 268, 429
MwoI GCNNNNNNNGC 2 cut(s) 121, 701
NdeI CATATG 1 cut(s) 464
NdeII GATC 2 cut(s) 304, 1082
NlaIII CATG 4 cut(s) 128, 305, 385, 611
NmuCI GTSAC 2 cut(s) 139, 565
OliI CACNNNNGTG 1 cut(s) 68
PceI AGGCCT 1 cut(s) 148
PctI GAATGC 2 cut(s) 268, 429
PfeI GAWTC 2 cut(s) 215, 440
PkrI GCNGC 1 cut(s) 660
Psp1406I AACGTT 1 cut(s) 249
PspFI CCCAGC 1 cut(s) 312
PstI CTGCAG 2 cut(s) 663, 865
PstNI CAGNNNCTG 1 cut(s) 563
RsaI GTAC 4 cut(s) 577, 685, 873, 1080
RsaNI GTAC 4 cut(s) 576, 684, 872, 1079
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 2 cut(s) 519, 551
SatI GCNGC 1 cut(s) 659
Sau3AI GATC 2 cut(s) 304, 1082
SduI GDGCHC 1 cut(s) 65
SfaNI GCATC 8 cut(s) 276, 318, 362, 550, 603, 978, 1000, 1030
SfcI CTRYAG 4 cut(s) 361, 571, 659, 861
SmiMI CAYNNNNRTG 1 cut(s) 68
Sse9I AATT 9 cut(s) 183, 377, 417, 469, 599, 641, 776, 944, 1046
SseBI AGGCCT 1 cut(s) 148
SspI AATATT 1 cut(s) 1101
SspMI CTAG 3 cut(s) 167, 171, 413
StuI AGGCCT 1 cut(s) 148
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 2 cut(s) 671, 998
TaiI ACGT 3 cut(s) 80, 252, 277
TasI AATT 9 cut(s) 183, 377, 417, 469, 599, 641, 776, 944, 1046
TfiI GAWTC 2 cut(s) 215, 440
Tru1I TTAA 2 cut(s) 519, 551
Tru9I TTAA 2 cut(s) 519, 551
TscAI CASTG 2 cut(s) 54, 676
TseFI GTSAC 2 cut(s) 139, 565
TseI GCWGC 1 cut(s) 658
Tsp45I GTSAC 2 cut(s) 139, 565
TspDTI ATGAA 7 cut(s) 370, 453, 551, 764, 771, 869, 1074
TspRI CASTG 2 cut(s) 54, 676
XapI RAATTY 4 cut(s) 377, 417, 599, 776
XcmI CCANNNNNNNNNTGG 1 cut(s) 54
XspI CTAG 3 cut(s) 167, 171, 413
ZraI GACGTC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.