Rw7G029860

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006792
Physical Location & Seq
Forward (+)
41830190 .. 41838212
8023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G029860.1

Sequence Viewer

Length: 1977 bp
ATGGAGGTGTCGGCACTCGGTGTTGGTGTCCAACATGTCCTCTGCTGCAACTCCGTTCCATTTATTCCCTCAGTGACGCCATGGAGCACAAGACTGAAGACTTCTCATGTTTGTTGTGCCACAACTCTTGACAAAGAGCCCCTCCGCCGGCAGCCTCCCAATTCTGACACTAGGAGGGTCACAAGGCCTCACTCCAAGCAATATTTGGCTAGACAATCGGCAATTCTTCAAGTCCAACAGTCCTCTGACTTGGAATCTGCTCTTACAAGATTAGGTGGAAGTTTGAAGGTTCAAGACTTGAATGCCATAATACGCCATTTTGGGATGCTGAAAAGATGGCATGATCTTTCCCAGCTCTTTGAATGGATGCAACAGAATGGAAAAATTAGTGCTTCATCATATAGCAGCTATATAAAATTCATGGGGAAGAGCCTCAATCCCGTAAAAGCACTAGAAATTTACAATAGCATTCAAGATGAATCAACCAAAAAGAACGTTCACATATGCAATTCGGTTCTTGGTAGTCTGGTCAGGAGTGGCAAGTTTGATGGCAGTATTAAGTTATTTCACCAGATGAAGCAGGATGGCTTAACACCAGATGCTGTTACCTATAGTACGCTGCTTGCAGGTTGCATCAAATTCAAACACGGTTATTCGAAGGCATTAGAGCTAGTTCAAGAATTACAGAATAATGAGCTGCAGATGGACAGGGTAATATATGGTACACTTTTGGCTATCTGTGCTTCAAATAATAAATGGGAGGAAGCAGAAAGTTATTTTAAGCAGATGAAGGATGAAGGTCATATACCAAATGAATTCCATTACAGCTCTTTGCTCAATGCTTACTCTATCAGCGGGAACTACAAAAAGGCTGATGATTTGGTGCAAGATATGAAATCTGCAGGGTTAGTACCTAATAAGGTTATTTTGACAACTTTATTGAAGGCTTATGTCAGAGGTGGGTTGTTTGAAAAATCAAGAGAATTATTAGCCGAACTGGAAGCTTTGGGATATGCTGAAGATGAGATGCCATACTGTCTATTGATGGATGCCCTTGCTAAGGCTGGGCATATACAAGATGCTAAATTGGTTTTTGATGAAATGAAGGAGAAATCTGTACGATCAGATGGATATTCCTACAGTATCATGATTTCTGCTTTCTGCCGAGGTGGGCTTGTAGATGATGCAAAGCAGTTGGCTAAGGATTTTGAAAGAACTTATGACAAATATGATTTGGTTATGTTGAATACAATGATCTGTGCCTATTGCAGAGCAGGTGAAATGGATAGTGTAATGGAAATGCTGAGAAAAATGGATGAATTAAAAATCATACCTGATAATAATACCTTCCATATTCTAATAAAGTACTTCTGTAAGGAGAAGTTATACATGCTTGCTTATAAGACCATGGAGGACATGCACAACAAAGGCTACCCATCAGATGAGGAACTTTGTTCCTCCTTAATGTTTCATCTTGGTAAGATAAGAGCATATTCAGAAGCATACTCTATTTACAATATGCTAAGGTATAGTAAGCGAACCATGTGCAAGGCCCTTCATGAGAAGATTCTACACATTCTAGTAGCAGGAAGGCTTCTCAAAGATGCTTATGTAGTAGTCAAGGATAATCCGGGGTTGATTTCAAAGCCTGCTACAATGAAATTTGCAACTGCATTTATGAAATTGGGAAATATCAACTTGATAAATGATGTTTTGAAGGCAATTGATGGTTCTGGTATCAAGATTGATCAGGGAATATTTCAGATGGCCATATCACGTTATATTTCGGACCCTGACAAGAAGGACCTGCTTCTTCAGTTACTACAGTGGATGCCGGGACAAGGTTATACTGTGGATTCATCTACAAGAAACCTAATACTGAAAAACTCTCACTTATTTGGTCGCCATCACATTGCCGAGATGCTATCAAAGCAGCATATGATATCAAAAGCTTCAAAATCTACTAAAGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

658

Amino Acids

74.57

Weight (kDa)

8.87

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 147 - 263 8.3e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 164 - 213 7.5e-13 PPR repeat family
PPR_long PF17177 218 - 357 3e-12 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 224 - 282 3.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 236 - 282 1.5e-08 PPR repeat family
PPR PF01535 238 - 267 9.4e-06 PPR repeat
PPR_2 PF13041 270 - 318 1.5e-07 PPR repeat family
PPR_2 PF13041 347 - 389 3.6e-11 PPR repeat family
PPR_1 PF12854 376 - 399 7.9e-06 PPR repeat
PPR_2 PF13041 411 - 459 5.5e-11 PPR repeat family
PPR PF01535 413 - 441 5.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1401
AarI CACCTGC 1 cut(s) 1265
Acc36I ACCTGC 3 cut(s) 617, 1265, 1815
AciI CCGC 2 cut(s) 145, 855
AclI AACGTT 1 cut(s) 495
AcoI YGGCCR 1 cut(s) 1767
AcsI RAATTY 5 cut(s) 416, 456, 638, 815, 1661
AcuI CTGAAG 3 cut(s) 116, 1038, 1799
AcyI GRCGYC 1 cut(s) 77
AdeI CACNNNGTG 1 cut(s) 20
AfaI GTAC 5 cut(s) 616, 724, 912, 1119, 1367
AfiI CCNNNNNNNGG 3 cut(s) 147, 1060, 1841
AflIII ACRYGT 1 cut(s) 34
AluBI AGCT 7 cut(s) 355, 408, 670, 697, 828, 1004, 1952
AluI AGCT 7 cut(s) 355, 408, 670, 697, 828, 1004, 1952
Alw21I GWGCWC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 602
AoxI GGCC 3 cut(s) 185, 1551, 1767
ApeKI GCWGC 6 cut(s) 45, 151, 405, 619, 697, 1933
ApoI RAATTY 5 cut(s) 416, 456, 638, 815, 1661
AspS9I GGNCC 3 cut(s) 1552, 1789, 1804
AsuC2I CCSGG 2 cut(s) 1632, 1836
AsuHPI GGTGA 2 cut(s) 560, 1289
AsuII TTCGAA 1 cut(s) 656
AvaII GGWCC 2 cut(s) 1789, 1804
BalI TGGCCA 1 cut(s) 1769
BanII GRGCYC 1 cut(s) 141
BarI GAAGNNNNNNTAC 2 cut(s) 789, 821
BbsI GAAGAC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 6 cut(s) 32, 163, 417, 606, 684, 1945
BcgI CGANNNNNNTGC 2 cut(s) 1527, 1561
BclI TGATCA 1 cut(s) 1747
BcnI CCSGG 2 cut(s) 1632, 1836
BfaI CTAG 5 cut(s) 171, 210, 452, 671, 1580
BfmI CTRYAG 5 cut(s) 610, 698, 900, 1138, 1823
BfuAI ACCTGC 3 cut(s) 617, 1265, 1815
BisI GCNGC 6 cut(s) 46, 152, 406, 620, 698, 1934
BlsI GCNGC 6 cut(s) 47, 153, 407, 621, 699, 1935
BmcAI AGTACT 1 cut(s) 1367
Bme1390I CCNGG 2 cut(s) 1632, 1836
Bme18I GGWCC 2 cut(s) 1789, 1804
BmgT120I GGNCC 3 cut(s) 1552, 1789, 1804
BmiI GGNNCC 1 cut(s) 1791
BmrFI CCNGG 2 cut(s) 1632, 1836
BpiI GAAGAC 1 cut(s) 104
Bpu10I CCTNAGC 3 cut(s) 1059, 1200, 1523
Bpu14I TTCGAA 1 cut(s) 656
BpuMI CCSGG 2 cut(s) 1632, 1836
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 80, 1165, 1407, 1631
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 1060, 1841
Bse118I RCCGGY 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 1002
Bse3DI GCAATG 1 cut(s) 1911
BseDI CCNNGG 4 cut(s) 80, 1165, 1407, 1631
BseGI GGATG 7 cut(s) 330, 372, 589, 799, 1054, 1321, 1836
BseLI CCNNNNNNNGG 3 cut(s) 147, 1060, 1841
BseMI GCAATG 1 cut(s) 1911
BseMII CTCAG 2 cut(s) 84, 1295
BseNI ACTGG 1 cut(s) 1002
BseXI GCAGC 6 cut(s) 32, 163, 417, 606, 684, 1945
BseYI CCCAGC 2 cut(s) 351, 1064
BshFI GGCC 3 cut(s) 187, 1553, 1769
BsiHKAI GWGCWC 1 cut(s) 89
BsiSI CCGG 3 cut(s) 148, 1631, 1835
BslFI GGGAC 1 cut(s) 1851
BslI CCNNNNNNNGG 3 cut(s) 147, 1060, 1841
BsmFI GGGAC 1 cut(s) 1851
BsmI GAATGC 2 cut(s) 307, 468
BsnI GGCC 3 cut(s) 187, 1553, 1769
Bsp119I TTCGAA 1 cut(s) 656
Bsp1286I GDGCHC 2 cut(s) 89, 141
Bsp143I GATC 4 cut(s) 343, 1121, 1254, 1747
Bsp19I CCATGG 2 cut(s) 80, 1407
BspACI CCGC 2 cut(s) 145, 855
BspANI GGCC 3 cut(s) 187, 1553, 1769
BspCNI CTCAG 2 cut(s) 83, 1296
BspHI TCATGA 2 cut(s) 1146, 1558
BspLI GGNNCC 1 cut(s) 1791
BspMAI CTGCAG 2 cut(s) 702, 904
BspMI ACCTGC 3 cut(s) 617, 1265, 1815
BspQI GCTCTTC 1 cut(s) 422
BspT104I TTCGAA 1 cut(s) 656
BsrDI GCAATG 1 cut(s) 1911
BsrFI RCCGGY 1 cut(s) 147
BsrI ACTGG 1 cut(s) 1002
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 4 cut(s) 80, 1165, 1407, 1631
BssMI GATC 4 cut(s) 343, 1121, 1254, 1747
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 2 cut(s) 80, 1407
Bst4CI ACNGT 6 cut(s) 240, 650, 1037, 1142, 1827, 1852
Bst6I CTCTTC 1 cut(s) 422
BstACI GRCGYC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 656
BstC8I GCNNGC 4 cut(s) 149, 624, 1395, 1650
BstDEI CTNAG 5 cut(s) 70, 1059, 1200, 1304, 1523
BstDSI CCRYGG 2 cut(s) 80, 1407
BstENI CCTNNNNNAGG 1 cut(s) 1058
BstF5I GGATG 7 cut(s) 330, 372, 589, 799, 1054, 1321, 1836
BstKTI GATC 4 cut(s) 346, 1124, 1257, 1750
BstMBI GATC 4 cut(s) 343, 1121, 1254, 1747
BstMWI GCNNNNNNNGC 2 cut(s) 740, 1930
BstNSI RCATGY 3 cut(s) 38, 1393, 1420
BstSCI CCNGG 2 cut(s) 1630, 1834
BstSFI CTRYAG 5 cut(s) 610, 698, 900, 1138, 1823
BstV1I GCAGC 6 cut(s) 32, 163, 417, 606, 684, 1945
BstV2I GAAGAC 1 cut(s) 104
BsuRI GGCC 3 cut(s) 187, 1553, 1769
BtgI CCRYGG 2 cut(s) 80, 1407
BtsCI GGATG 7 cut(s) 330, 372, 589, 799, 1054, 1321, 1836
BtsIMutI CAGTG 2 cut(s) 78, 1832
BveI ACCTGC 3 cut(s) 617, 1265, 1815
Cac8I GCNNGC 4 cut(s) 149, 624, 1395, 1650
CaiI CAGNNNCTG 1 cut(s) 602
CciI TCATGA 2 cut(s) 1146, 1558
Cfr10I RCCGGY 1 cut(s) 147
Cfr13I GGNCC 3 cut(s) 1552, 1789, 1804
CseI GACGC 1 cut(s) 85
Csp6I GTAC 5 cut(s) 615, 723, 911, 1118, 1366
CspCI CAANNNNNGTGG 2 cut(s) 109, 144
CviQI GTAC 5 cut(s) 615, 723, 911, 1118, 1366
DdeI CTNAG 5 cut(s) 70, 1059, 1200, 1304, 1523
DpnI GATC 4 cut(s) 345, 1123, 1256, 1749
DpnII GATC 4 cut(s) 343, 1121, 1254, 1747
DraIII CACNNNGTG 1 cut(s) 20
EaeI YGGCCR 1 cut(s) 1767
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
EciI GGCGGA 1 cut(s) 134
Eco130I CCWWGG 2 cut(s) 80, 1407
Eco147I AGGCCT 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 141
Eco32I GATATC 1 cut(s) 1944
Eco47I GGWCC 2 cut(s) 1789, 1804
Eco57I CTGAAG 3 cut(s) 116, 1038, 1799
EcoNI CCTNNNNNAGG 1 cut(s) 1058
EcoO109I RGGNCCY 2 cut(s) 1552, 1804
EcoRI GAATTC 1 cut(s) 815
EcoRV GATATC 1 cut(s) 1944
EcoT14I CCWWGG 2 cut(s) 80, 1407
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 80, 1407
FaqI GGGAC 1 cut(s) 1851
FauI CCCGC 1 cut(s) 848
FauNDI CATATG 2 cut(s) 503, 1938
FbaI TGATCA 1 cut(s) 1747
Fnu4HI GCNGC 6 cut(s) 46, 152, 406, 620, 698, 1934
FokI GGATG 7 cut(s) 337, 379, 596, 806, 1061, 1328, 1843
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 6 cut(s) 46, 152, 406, 620, 698, 1934
FspBI CTAG 5 cut(s) 171, 210, 452, 671, 1580
GluI GCNGC 6 cut(s) 46, 152, 406, 620, 698, 1934
GsaI CCCAGC 2 cut(s) 355, 1068
HaeIII GGCC 3 cut(s) 187, 1553, 1769
HapII CCGG 3 cut(s) 148, 1631, 1835
HgaI GACGC 1 cut(s) 85
Hin1I GRCGYC 1 cut(s) 77
HindIII AAGCTT 2 cut(s) 1002, 1950
HinfI GANTC 4 cut(s) 254, 479, 1567, 1856
HpaII CCGG 3 cut(s) 148, 1631, 1835
HphI GGTGA 2 cut(s) 560, 1289
Hpy166II GTNNAC 2 cut(s) 499, 725
Hpy188I TCNGA 8 cut(s) 166, 247, 956, 1126, 1441, 1498, 1764, 1789
Hpy188III TCNNGA 9 cut(s) 128, 293, 473, 532, 677, 978, 1147, 1559, 1741
Hpy8I GTNNAC 2 cut(s) 499, 725
HpyCH4III ACNGT 6 cut(s) 240, 650, 1037, 1142, 1827, 1852
HpyCH4IV ACGT 2 cut(s) 495, 1777
HpyF10VI GCNNNNNNNGC 2 cut(s) 740, 1930
HpyF3I CTNAG 5 cut(s) 70, 1059, 1200, 1304, 1523
HpySE526I ACGT 2 cut(s) 495, 1777
Hsp92I GRCGYC 1 cut(s) 77
KroI GCCGGC 1 cut(s) 147
KroNI GCCGGC 1 cut(s) 149
Ksp22I TGATCA 1 cut(s) 1747
Kzo9I GATC 4 cut(s) 343, 1121, 1254, 1747
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 6 cut(s) 32, 163, 417, 606, 684, 1945
MaeI CTAG 5 cut(s) 171, 210, 452, 671, 1580
MaeII ACGT 2 cut(s) 495, 1777
MaeIII GTNAC 4 cut(s) 73, 178, 604, 1818
MalI GATC 4 cut(s) 345, 1123, 1256, 1749
MboI GATC 4 cut(s) 343, 1121, 1254, 1747
MboII GAAGA 6 cut(s) 109, 218, 439, 1031, 1576, 1805
MfeI CAATTG 1 cut(s) 1722
MhlI GDGCHC 2 cut(s) 89, 141
MlsI TGGCCA 1 cut(s) 1769
MluNI TGGCCA 1 cut(s) 1769
MmeI TCCRAC 2 cut(s) 55, 259
Mox20I TGGCCA 1 cut(s) 1769
MroNI GCCGGC 1 cut(s) 147
MscI TGGCCA 1 cut(s) 1769
MseI TTAA 5 cut(s) 558, 590, 780, 1322, 1463
Msp20I TGGCCA 1 cut(s) 1769
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 3 cut(s) 148, 1631, 1835
MspR9I CCNGG 2 cut(s) 1632, 1836
MunI CAATTG 1 cut(s) 1722
Mva1269I GAATGC 2 cut(s) 307, 468
MwoI GCNNNNNNNGC 2 cut(s) 740, 1930
NaeI GCCGGC 1 cut(s) 149
NciI CCSGG 2 cut(s) 1632, 1836
NcoI CCATGG 2 cut(s) 80, 1407
NdeI CATATG 2 cut(s) 503, 1938
NdeII GATC 4 cut(s) 343, 1121, 1254, 1747
NgoMIV GCCGGC 1 cut(s) 147
NlaIV GGNNCC 1 cut(s) 1791
NmeAIII GCCGAG 2 cut(s) 1190, 1942
NmuCI GTSAC 2 cut(s) 73, 178
NspI RCATGY 3 cut(s) 38, 1393, 1420
NspV TTCGAA 1 cut(s) 656
PagI TCATGA 2 cut(s) 1146, 1558
PaqCI CACCTGC 1 cut(s) 1265
PceI AGGCCT 1 cut(s) 187
PciI ACATGT 1 cut(s) 34
PciSI GCTCTTC 1 cut(s) 422
PctI GAATGC 2 cut(s) 307, 468
PdiI GCCGGC 1 cut(s) 149
PfeI GAWTC 4 cut(s) 254, 479, 1567, 1856
PkrI GCNGC 6 cut(s) 47, 153, 407, 621, 699, 1935
PpuMI RGGWCCY 1 cut(s) 1804
PscI ACATGT 1 cut(s) 34
PsiI TTATAA 1 cut(s) 1401
Psp1406I AACGTT 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 1804
PspFI CCCAGC 2 cut(s) 351, 1064
PspN4I GGNNCC 1 cut(s) 1791
PspPI GGNCC 3 cut(s) 1552, 1789, 1804
PspPPI RGGWCCY 1 cut(s) 1804
PstI CTGCAG 2 cut(s) 702, 904
PstNI CAGNNNCTG 1 cut(s) 602
RsaI GTAC 5 cut(s) 616, 724, 912, 1119, 1367
RsaNI GTAC 5 cut(s) 615, 723, 911, 1118, 1366
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 5 cut(s) 558, 590, 780, 1322, 1463
SatI GCNGC 6 cut(s) 46, 152, 406, 620, 698, 1934
Sau3AI GATC 4 cut(s) 343, 1121, 1254, 1747
Sau96I GGNCC 3 cut(s) 1552, 1789, 1804
ScaI AGTACT 1 cut(s) 1367
ScrFI CCNGG 2 cut(s) 1632, 1836
SduI GDGCHC 2 cut(s) 89, 141
SfcI CTRYAG 5 cut(s) 610, 698, 900, 1138, 1823
SfuI TTCGAA 1 cut(s) 656
SinI GGWCC 2 cut(s) 1789, 1804
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 2 cut(s) 145, 855
SspI AATATT 2 cut(s) 203, 1758
SspMI CTAG 5 cut(s) 171, 210, 452, 671, 1580
StuI AGGCCT 1 cut(s) 187
StyD4I CCNGG 2 cut(s) 1630, 1834
StyI CCWWGG 2 cut(s) 80, 1407
TaaI ACNGT 6 cut(s) 240, 650, 1037, 1142, 1827, 1852
TaiI ACGT 2 cut(s) 498, 1780
TaqI TCGA 1 cut(s) 656
TatI WGTACW 1 cut(s) 1365
TfiI GAWTC 4 cut(s) 254, 479, 1567, 1856
Tru1I TTAA 5 cut(s) 558, 590, 780, 1322, 1463
Tru9I TTAA 5 cut(s) 558, 590, 780, 1322, 1463
TscAI CASTG 2 cut(s) 78, 1832
TseFI GTSAC 2 cut(s) 73, 178
TseI GCWGC 6 cut(s) 45, 151, 405, 619, 697, 1933
Tsp45I GTSAC 2 cut(s) 73, 178
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 2 cut(s) 78, 1832
VpaK11BI GGWCC 2 cut(s) 1789, 1804
XagI CCTNNNNNAGG 1 cut(s) 1058
XapI RAATTY 5 cut(s) 416, 456, 638, 815, 1661
XceI RCATGY 3 cut(s) 38, 1393, 1420
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XspI CTAG 5 cut(s) 171, 210, 452, 671, 1580
ZrmI AGTACT 1 cut(s) 1367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.