MD15G1423900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006792
Physical Location & Seq
Forward (+)
52482973 .. 52491810
8838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1423900.v1.1.491

Sequence Viewer

Length: 1995 bp
ATGGAGGTGACGTCGGTACAGGGCGTCGCCGGCGGTGTCCAGCACGTCCTCTGCGGCCCCTCCTCGCCCATTTCTTCTCTATCCATTCTCTCTCCGACTCCATGGAGGAGCGCAAGAGCCAAGAATTCTCATCTCTGTTGTGCCACAACTCTTGTGGGGGAGGCTCCCAACAATGGCAGTAACAGGGTCCCGAAGCGTCACTCCAAGCACTACTTAGCCAGACAGTCTGCAATTGTTCAAGTCCAAGACTCCTCCGACTTGGGCTCCGCTCTCACAAGATTAGGTGGGAGTTTGACGGTTCAAGACTTGAATGCCATTATACGCCATTTGGGGACGAAGAAAAGATGGCATGATCTTTCCCAGCTCTTTGAATGGATGCTACAGAATGAAAAAGTCAGTGCATCATCCTATAGCAGCTATATAAAGTTCATGGGAAAGAGCCTCAATCCTGTAAAAGCACTAGAAATATACAATAACATTCAAGATGAATCAACCAAAAAGAATGTTCACATATGCAATTCTGTTCTTGGTAGTCTGATCAGGAGTGGCAAGTTTGATGTCAGCTTTAAGTTATTTGACCAGATGAAGCAGAATGGTTTAACGCCAGATGCTGTCACATATAGTACGCTGCTTGCAGGTTGCAGCAAACTCAAAAATGGTTATAATAAGGCATTAGAATTTGTTCAAGAATTGCAGCGTAATGAGCTGCAGATGGACAGTGTAATTTATGGTACACTTTTAGCTGTCTGTGCATCTAATAATAAATTGGAGGAAGCAGAAGGTTATTTTAAGAAGATGAAGGATGAGGGTCATTCACCAAATGTCTACCACTACAGCTCTTTGCTTAATGCGTACTCTATGAGAGGGAACTACAAGGAGGCTGATGTTTTGGTTCAAGATATGAAATCTGCAGGGTTAGTACCAAATAAGGTTATTTTGACAACTTTATTGAAGGTTTATGTCAGAGGGGGGCTGTTTGAAAAATCAAGAGAACTATTAGGTGAACTGGAAGCTTTGGGTTATGCTGAAGACGAGATGCCGTACTGTTTATTGATGGATGCCCTTGCGAAGGCTGGGCGAATACATGAAGCTAAGTTGGTTTTTCAAGAAATGAAGGAGAAGTCCGTCCGATCAGATGGCTATTCTTACAGTATCATGATTTCCGCTTTCTGCCGAGGTGGGCTTCTGGAAGATGCAAAGCAGCTGGCAAAGGATTTTGAAGGAACCCATGATAGATATGATTTAGTTATGTTGAATACAATGATCTGTGCCTATTGCAGAGCAGGCGATATGGATAGTGTAATGGAAATGATGAGAAAAATGGATGAACTAAAAATCACTCCTGATTATAATACCTTTCATATCCTCATAAAATACTTCTGCAAGGAGAAGTTATACCTTCTTGCTTATCAGACCATGGAGGACATGCACAACAAGGGCCACCAACCAGATGAGGAACTGTGTTCCTCCTTAATGTTTCTTCTTGGTAAGATAAGAGCATATTCAGAAGCATACTCTGTTTACAATATGCTGAGATATAGCAAGCGGACAATGTGCAAGGCCCTTCATGAGAAGATTCTGCACATTCTAGTAGCAGGACAGCTTCTCAGAGATGCTTATGTAGTAGTCAAGGACAATGCAGGGTTAATTTCTAAGCCTGCTATTAAAAAGTTTGCAACTGCCTTTATGAAGTTGGGAAACATCAACTTGATAAATGATGTTTTGAAGGCGGTTGATGCTTCTGGCTGTAAGATAGATCAGGGACTATTTCAGATGGCCATATCTCGCTATGTTTCACTCCCTGAGAAGAAGGACATGCTTTTTCAAATGCTACAGTGGATGCCAGGACAAGGTTATGTTGCTGATTCAGCTACAAGAAACGTGATACTGAAAAACTCTCACTTGTTTGGTCGCCAACAGATTGCGGAGATACTCTCAAAGCAGCATATGATTTCAAAAGCTTCAAAATCTCGCGAGAAGGAGAAAAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

665

Amino Acids

74.63

Weight (kDa)

9.0

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 151 - 266 1.3e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 156 - 214 3.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 167 - 216 8.2e-14 PPR repeat family
PPR_long PF17177 209 - 363 3.1e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 228 - 285 2.6e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 239 - 285 7.6e-10 PPR repeat family
PPR_3 PF13812 264 - 321 6.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 273 - 320 3.3e-07 PPR repeat family
PPR_2 PF13041 350 - 392 5e-11 PPR repeat family
PPR_3 PF13812 412 - 461 7.9e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 414 - 462 2.4e-10 PPR repeat family
PPR PF01535 416 - 444 7.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 663, 1350
AatII GACGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 626
AccBSI CCGCTC 1 cut(s) 269
AccI GTMKAC 1 cut(s) 825
AccII CGCG 1 cut(s) 1974
AciI CCGC 7 cut(s) 33, 54, 267, 1164, 1546, 1730, 1925
AcoI YGGCCR 1 cut(s) 1776
AcsI RAATTY 2 cut(s) 124, 677
AcuI CTGAAG 1 cut(s) 1047
AcyI GRCGYC 2 cut(s) 11, 24
AfaI GTAC 6 cut(s) 18, 625, 733, 854, 921, 1043
AfiI CCNNNNNNNGG 3 cut(s) 173, 1069, 1850
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 1 cut(s) 1843
AlwNI CAGNNNCTG 1 cut(s) 611
AoxI GGCC 4 cut(s) 55, 1438, 1560, 1776
ApeKI GCWGC 7 cut(s) 414, 628, 642, 694, 706, 1201, 1942
ApoI RAATTY 2 cut(s) 124, 677
Asp700I GAANNNNTTC 1 cut(s) 681
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 4 cut(s) 56, 187, 1438, 1561
AsuHPI GGTGA 3 cut(s) 19, 807, 1013
AvaII GGWCC 1 cut(s) 187
BalI TGGCCA 1 cut(s) 1778
BanII GRGCYC 1 cut(s) 266
BbsI GAAGAC 1 cut(s) 1035
BbvI GCAGC 7 cut(s) 426, 615, 654, 693, 706, 1213, 1954
BccI CCATC 5 cut(s) 339, 706, 1048, 1130, 1768
BceAI ACGGC 1 cut(s) 1024
BciT130I CCWGG 1 cut(s) 1845
BclI TGATCA 1 cut(s) 537
BfaI CTAG 2 cut(s) 461, 1589
BfmI CTRYAG 6 cut(s) 380, 409, 707, 832, 909, 1832
BfuAI ACCTGC 1 cut(s) 626
BisI GCNGC 8 cut(s) 55, 415, 629, 643, 695, 707, 1202, 1943
BlsI GCNGC 8 cut(s) 56, 416, 630, 644, 696, 708, 1203, 1944
Bme1390I CCNGG 1 cut(s) 1845
Bme18I GGWCC 1 cut(s) 187
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 4 cut(s) 56, 187, 1438, 1561
BmiI GGNNCC 6 cut(s) 58, 165, 188, 189, 265, 1225
BmrFI CCNGG 1 cut(s) 1845
BpiI GAAGAC 1 cut(s) 1035
BplI GAGNNNNNCTC 2 cut(s) 1919, 1951
BsaHI GRCGYC 2 cut(s) 11, 24
BsaJI CCNNGG 3 cut(s) 101, 1174, 1416
BsaXI ACNNNNNCTCC 3 cut(s) 26, 248, 278
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 1069, 1850
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 1011
BseBI CCWGG 1 cut(s) 1845
BseDI CCNNGG 3 cut(s) 101, 1174, 1416
BseGI GGATG 6 cut(s) 381, 404, 808, 1063, 1330, 1845
BseLI CCNNNNNNNGG 3 cut(s) 173, 1069, 1850
BseMII CTCAG 3 cut(s) 1523, 1621, 1794
BseNI ACTGG 1 cut(s) 1011
BseRI GAGGAG 3 cut(s) 52, 121, 241
BseXI GCAGC 7 cut(s) 426, 615, 654, 693, 706, 1213, 1954
BseYI CCCAGC 2 cut(s) 360, 1073
BsgI GTGCAG 1 cut(s) 1565
Bsh1236I CGCG 1 cut(s) 1974
BshFI GGCC 4 cut(s) 57, 1440, 1562, 1778
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 3 cut(s) 173, 346, 1776
BslI CCNNNNNNNGG 3 cut(s) 173, 1069, 1850
BsmFI GGGAC 3 cut(s) 173, 346, 1776
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 4 cut(s) 57, 1440, 1562, 1778
Bsp1286I GDGCHC 1 cut(s) 266
Bsp143I GATC 5 cut(s) 352, 537, 1130, 1263, 1756
Bsp19I CCATGG 2 cut(s) 101, 1416
Bsp68I TCGCGA 1 cut(s) 1974
BspACI CCGC 7 cut(s) 33, 54, 267, 1164, 1546, 1730, 1925
BspANI GGCC 4 cut(s) 57, 1440, 1562, 1778
BspCNI CTCAG 3 cut(s) 1524, 1620, 1795
BspFNI CGCG 1 cut(s) 1974
BspHI TCATGA 2 cut(s) 1155, 1567
BspLI GGNNCC 6 cut(s) 58, 165, 188, 189, 265, 1225
BspMAI CTGCAG 2 cut(s) 711, 913
BspMI ACCTGC 1 cut(s) 626
BsrBI CCGCTC 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 1 cut(s) 1011
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 3 cut(s) 101, 1174, 1416
BssMI GATC 5 cut(s) 352, 537, 1130, 1263, 1756
BssNI GRCGYC 2 cut(s) 11, 24
BssT1I CCWWGG 2 cut(s) 101, 1416
Bst2UI CCWGG 1 cut(s) 1845
Bst4CI ACNGT 7 cut(s) 225, 298, 719, 1046, 1151, 1461, 1836
BstACI GRCGYC 2 cut(s) 11, 24
BstC8I GCNNGC 6 cut(s) 31, 633, 1206, 1285, 1544, 1659
BstDEI CTNAG 6 cut(s) 214, 1092, 1532, 1607, 1653, 1803
BstDSI CCRYGG 2 cut(s) 101, 1416
BstENI CCTNNNNNAGG 1 cut(s) 1067
BstF5I GGATG 6 cut(s) 381, 404, 808, 1063, 1330, 1845
BstFNI CGCG 1 cut(s) 1974
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 5 cut(s) 355, 540, 1133, 1266, 1759
BstMBI GATC 5 cut(s) 352, 537, 1130, 1263, 1756
BstMWI GCNNNNNNNGC 6 cut(s) 30, 703, 749, 1284, 1736, 1868
BstNI CCWGG 1 cut(s) 1845
BstNSI RCATGY 2 cut(s) 1429, 1819
BstSCI CCNGG 1 cut(s) 1843
BstSFI CTRYAG 6 cut(s) 380, 409, 707, 832, 909, 1832
BstUI CGCG 1 cut(s) 1974
BstV1I GCAGC 7 cut(s) 426, 615, 654, 693, 706, 1213, 1954
BstV2I GAAGAC 1 cut(s) 1035
BsuRI GGCC 4 cut(s) 57, 1440, 1562, 1778
BtgI CCRYGG 2 cut(s) 101, 1416
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 6 cut(s) 381, 404, 808, 1063, 1330, 1845
BtsIMutI CAGTG 3 cut(s) 403, 724, 1841
BtuMI TCGCGA 1 cut(s) 1974
BveI ACCTGC 1 cut(s) 626
Cac8I GCNNGC 6 cut(s) 31, 633, 1206, 1285, 1544, 1659
CaiI CAGNNNCTG 1 cut(s) 611
CciI TCATGA 2 cut(s) 1155, 1567
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 4 cut(s) 56, 187, 1438, 1561
CseI GACGC 2 cut(s) 13, 185
Csp6I GTAC 6 cut(s) 17, 624, 732, 853, 920, 1042
CspCI CAANNNNNGTGG 4 cut(s) 133, 135, 168, 170
CviQI GTAC 6 cut(s) 17, 624, 732, 853, 920, 1042
DdeI CTNAG 6 cut(s) 214, 1092, 1532, 1607, 1653, 1803
DpnI GATC 5 cut(s) 354, 539, 1132, 1265, 1758
DpnII GATC 5 cut(s) 352, 537, 1130, 1263, 1756
EaeI YGGCCR 1 cut(s) 1776
Eco130I CCWWGG 2 cut(s) 101, 1416
Eco24I GRGCYC 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 1047
EcoNI CCTNNNNNAGG 1 cut(s) 1067
EcoO109I RGGNCCY 2 cut(s) 187, 1561
EcoRI GAATTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 1843
EcoT14I CCWWGG 2 cut(s) 101, 1416
EcoT38I GRGCYC 1 cut(s) 266
ErhI CCWWGG 2 cut(s) 101, 1416
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 3 cut(s) 173, 346, 1776
FauNDI CATATG 2 cut(s) 512, 1947
FbaI TGATCA 1 cut(s) 537
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 8 cut(s) 55, 415, 629, 643, 695, 707, 1202, 1943
FokI GGATG 6 cut(s) 388, 391, 815, 1070, 1337, 1852
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 8 cut(s) 55, 415, 629, 643, 695, 707, 1202, 1943
FspBI CTAG 2 cut(s) 461, 1589
GlaI GCGC 1 cut(s) 112
GluI GCNGC 8 cut(s) 55, 415, 629, 643, 695, 707, 1202, 1943
GsaI CCCAGC 2 cut(s) 364, 1077
HaeIII GGCC 4 cut(s) 57, 1440, 1562, 1778
HapII CCGG 1 cut(s) 30
HgaI GACGC 2 cut(s) 13, 185
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 2 cut(s) 11, 24
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HindIII AAGCTT 2 cut(s) 1011, 1959
HinfI GANTC 5 cut(s) 97, 248, 488, 1576, 1865
HpaII CCGG 1 cut(s) 30
HphI GGTGA 3 cut(s) 19, 807, 1013
Hpy166II GTNNAC 5 cut(s) 508, 734, 826, 1004, 1522
Hpy8I GTNNAC 5 cut(s) 508, 734, 826, 1004, 1522
Hpy99I CGWCG 2 cut(s) 16, 29
HpyCH4III ACNGT 7 cut(s) 225, 298, 719, 1046, 1151, 1461, 1836
HpyCH4IV ACGT 3 cut(s) 11, 45, 1881
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 703, 749, 1284, 1736, 1868
HpyF3I CTNAG 6 cut(s) 214, 1092, 1532, 1607, 1653, 1803
HpySE526I ACGT 3 cut(s) 11, 45, 1881
Hsp92I GRCGYC 2 cut(s) 11, 24
HspAI GCGC 1 cut(s) 111
KflI GGGWCCC 1 cut(s) 187
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 5 cut(s) 352, 537, 1130, 1263, 1756
LmnI GCTCC 3 cut(s) 108, 169, 269
Lsp1109I GCAGC 7 cut(s) 426, 615, 654, 693, 706, 1213, 1954
MaeI CTAG 2 cut(s) 461, 1589
MaeII ACGT 3 cut(s) 11, 45, 1881
MaeIII GTNAC 4 cut(s) 7, 179, 197, 613
MalI GATC 5 cut(s) 354, 539, 1132, 1265, 1758
MbiI CCGCTC 1 cut(s) 269
MboI GATC 5 cut(s) 352, 537, 1130, 1263, 1756
MboII GAAGA 8 cut(s) 66, 349, 805, 1040, 1202, 1472, 1585, 1819
MfeI CAATTG 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 266
MlsI TGGCCA 1 cut(s) 1778
MluCI AATT 8 cut(s) 124, 231, 517, 677, 689, 723, 764, 1647
MluNI TGGCCA 1 cut(s) 1778
MlyI GAGTC 2 cut(s) 91, 242
MmeI TCCRAC 2 cut(s) 119, 279
Mox20I TGGCCA 1 cut(s) 1778
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 681
MscI TGGCCA 1 cut(s) 1778
MseI TTAA 7 cut(s) 567, 599, 789, 846, 1472, 1646, 1665
Msp20I TGGCCA 1 cut(s) 1778
MspA1I CMGCKG 1 cut(s) 1204
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 1845
MunI CAATTG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 316
MvaI CCWGG 1 cut(s) 1845
MvnI CGCG 1 cut(s) 1974
MwoI GCNNNNNNNGC 6 cut(s) 30, 703, 749, 1284, 1736, 1868
NaeI GCCGGC 1 cut(s) 31
NcoI CCATGG 2 cut(s) 101, 1416
NdeI CATATG 2 cut(s) 512, 1947
NdeII GATC 5 cut(s) 352, 537, 1130, 1263, 1756
NgoMIV GCCGGC 1 cut(s) 29
NlaIV GGNNCC 6 cut(s) 58, 165, 188, 189, 265, 1225
NmeAIII GCCGAG 1 cut(s) 1199
NmuCI GTSAC 3 cut(s) 7, 197, 613
NruI TCGCGA 1 cut(s) 1974
NspI RCATGY 2 cut(s) 1429, 1819
PagI TCATGA 2 cut(s) 1155, 1567
PctI GAATGC 1 cut(s) 316
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 3 cut(s) 488, 1576, 1865
PkrI GCNGC 8 cut(s) 56, 416, 630, 644, 696, 708, 1203, 1944
PleI GAGTC 2 cut(s) 91, 242
PpsI GAGTC 2 cut(s) 91, 242
PpuMI RGGWCCY 1 cut(s) 187
PsiI TTATAA 2 cut(s) 663, 1350
Psp5II RGGWCCY 1 cut(s) 187
Psp6I CCWGG 1 cut(s) 1843
PspFI CCCAGC 2 cut(s) 360, 1073
PspGI CCWGG 1 cut(s) 1843
PspN4I GGNNCC 6 cut(s) 58, 165, 188, 189, 265, 1225
PspPI GGNCC 4 cut(s) 56, 187, 1438, 1561
PspPPI RGGWCCY 1 cut(s) 187
PstI CTGCAG 2 cut(s) 711, 913
PstNI CAGNNNCTG 1 cut(s) 611
PvuII CAGCTG 1 cut(s) 1204
RruI TCGCGA 1 cut(s) 1974
RsaI GTAC 6 cut(s) 18, 625, 733, 854, 921, 1043
RsaNI GTAC 6 cut(s) 17, 624, 732, 853, 920, 1042
SaqAI TTAA 7 cut(s) 567, 599, 789, 846, 1472, 1646, 1665
SatI GCNGC 8 cut(s) 55, 415, 629, 643, 695, 707, 1202, 1943
Sau3AI GATC 5 cut(s) 352, 537, 1130, 1263, 1756
Sau96I GGNCC 4 cut(s) 56, 187, 1438, 1561
SchI GAGTC 2 cut(s) 91, 242
ScrFI CCNGG 1 cut(s) 1845
SduI GDGCHC 1 cut(s) 266
SfcI CTRYAG 6 cut(s) 380, 409, 707, 832, 909, 1832
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 187
Sse9I AATT 8 cut(s) 124, 231, 517, 677, 689, 723, 764, 1647
SsiI CCGC 7 cut(s) 33, 54, 267, 1164, 1546, 1730, 1925
SspMI CTAG 2 cut(s) 461, 1589
StyD4I CCNGG 1 cut(s) 1843
StyI CCWWGG 2 cut(s) 101, 1416
TaaI ACNGT 7 cut(s) 225, 298, 719, 1046, 1151, 1461, 1836
TaiI ACGT 3 cut(s) 14, 48, 1884
TasI AATT 8 cut(s) 124, 231, 517, 677, 689, 723, 764, 1647
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 3 cut(s) 488, 1576, 1865
Tru1I TTAA 7 cut(s) 567, 599, 789, 846, 1472, 1646, 1665
Tru9I TTAA 7 cut(s) 567, 599, 789, 846, 1472, 1646, 1665
TscAI CASTG 3 cut(s) 403, 724, 1841
TseFI GTSAC 3 cut(s) 7, 197, 613
TseI GCWGC 7 cut(s) 414, 628, 642, 694, 706, 1201, 1942
Tsp45I GTSAC 3 cut(s) 7, 197, 613
TspGWI ACGGA 1 cut(s) 1114
TspRI CASTG 3 cut(s) 403, 724, 1841
VpaK11BI GGWCC 1 cut(s) 187
XagI CCTNNNNNAGG 1 cut(s) 1067
XapI RAATTY 2 cut(s) 124, 677
XceI RCATGY 2 cut(s) 1429, 1819
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmiI GTMKAC 1 cut(s) 825
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 2 cut(s) 461, 1589
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.