FvH4_5g30330

Cyclic pyranopterin monophosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002342
Physical Location & Seq
Forward (+)
21223245 .. 21228876
5632 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30330.t9

Sequence Viewer

Length: 1122 bp
ATGATGATGATGATGAAGCGTCATCCATTAAAGCTTCCCCACTTGCGCACACGCTTTGCAAATCTACATTCTCTCCTGTTATCCCCTTATGGAACTACTTCTTGTGCGAAGTTGCCGGAGGAACACAACCCTGTGTCGAATATGTTGATCGATTCCTTCGGAAGGCATCACACTTACTTGAGGATCTCCTTGACAGAGCGGTGCAATCTGCGATGTCAATACTGTATGCCAGCCGAGGGTGTCAGCCTCACTCCTAACTCTAACATTCTCTCACACACTGAGCTCCTTCGATTGGCTGGTCTGTTCGTAAGCTCCGGAGTGGATAAGATTCGTTTGACTGGAGGGGAGCCAACCGTGAGGAAAGATATCGAAGACATCTGCTTGCAGTTGTCTAACTTGAAGGGATTGAAGACCCTAGCTGTCACTACTAATGGCATTACTCTTGCAAGGAAACTTCCCAAGCTGAAAGAATGCGGCCTTAGTGCTGTCAATATTAGTTTAGACACCCTGGTCCCTGCAAAGTTTGAGTTTCTGACAAGGCGTAAAGGGCATGAAAGGGTTATGGACTCGATTAATGCTGCCATTGACCTCGGTTACAATCCTGTTAAGGTAAATTGCGTTGTAATGCGCGGGTTCAACGATGATGAGATTTGTGATTTTGTGGAGCTGACACGTGAAAAGCCAATTAACGTTCGATTCATTGAGTTCATGCCTTTTGATGGAAATGTATGGAATGTTAAGAAACTTGTACCCTACGCGGAAATGTTGGATAGAGTGGCAACACATTATACAGGCCTGAAAAGACTTCAGGATCACCCAACTGATACTGCCAAGAATTTCAGGATAGATGATTATCTTGGTACAGTTTCTTTTATTACATCAATGACTGAGCATTTTTGTGCTGGTTGCAATAGATTGCGACTCTTAGCTGATGGGAACTTCAAAGTTTGCTTGTTTGGTCCTTCAGAGGTCAGCTTAAGAGATCCCCTTAGGCAGGGTGCTGATGATCATGAACTAGCAGAAATTATTGGTTCAGCGGTGAAGAGGAAGAAAGCATCGCATGCTGGAATGTTTGACATTGCAAAGACAGCAAATAGGCCGATGATACATATCGGTGGCTAA

Protein Analysis

374

Amino Acids

41.84

Weight (kDa)

8.98

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 62 - 223 8.1e-34 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 509
Acc16I TGCGCA 1 cut(s) 47
AccBSI CCGCTC 1 cut(s) 199
AccII CGCG 2 cut(s) 630, 758
AccIII TCCGGA 1 cut(s) 314
AciI CCGC 5 cut(s) 199, 474, 630, 758, 1037
AclI AACGTT 1 cut(s) 690
AclWI GGATC 3 cut(s) 191, 819, 977
AcsI RAATTY 1 cut(s) 835
AcuI CTGAAG 2 cut(s) 791, 948
AcvI CACGTG 1 cut(s) 674
AfaI GTAC 2 cut(s) 750, 862
AfiI CCNNNNNNNGG 5 cut(s) 162, 236, 292, 719, 994
AflII CTTAAG 1 cut(s) 976
AflIII ACRYGT 1 cut(s) 671
AgsI TTSAA 4 cut(s) 400, 409, 637, 943
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 8 cut(s) 34, 283, 312, 419, 463, 667, 929, 975
AluI AGCT 8 cut(s) 34, 283, 312, 419, 463, 667, 929, 975
Alw21I GWGCWC 1 cut(s) 285
AlwI GGATC 3 cut(s) 191, 819, 977
Aor13HI TCCGGA 1 cut(s) 314
AoxI GGCC 3 cut(s) 475, 793, 1097
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 1 cut(s) 835
AseI ATTAAT 1 cut(s) 573
Asp700I GAANNNNTTC 1 cut(s) 97
AspLEI GCGC 2 cut(s) 48, 630
AspS9I GGNCC 2 cut(s) 511, 959
AsuHPI GGTGA 2 cut(s) 806, 1051
AvaII GGWCC 2 cut(s) 511, 959
AxyI CCTNAGG 1 cut(s) 989
BanII GRGCYC 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 674
BbsI GAAGAC 2 cut(s) 378, 416
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 565
BccI CCATC 2 cut(s) 713, 926
BciT130I CCWGG 1 cut(s) 509
BclI TGATCA 1 cut(s) 1006
BfaI CTAG 2 cut(s) 416, 1016
BfrI CTTAAG 1 cut(s) 976
BisI GCNGC 2 cut(s) 475, 579
BlsI GCNGC 2 cut(s) 476, 580
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 2 cut(s) 511, 959
BmgT120I GGNCC 2 cut(s) 511, 959
BmiI GGNNCC 2 cut(s) 348, 513
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 2 cut(s) 175, 1064
BpiI GAAGAC 2 cut(s) 378, 416
BpmI CTGGAG 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 199
Bsa29I ATCGAT 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 674
BsaBI GATNNNNATC 2 cut(s) 852, 1109
BsaJI CCNNGG 3 cut(s) 234, 507, 589
BsaWI WCCGGW 1 cut(s) 314
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 5 cut(s) 162, 236, 292, 719, 994
Bse1I ACTGG 1 cut(s) 343
Bse21I CCTNAGG 1 cut(s) 989
Bse3DI GCAATG 1 cut(s) 1077
Bse8I GATNNNNATC 2 cut(s) 852, 1109
BseAI TCCGGA 1 cut(s) 314
BseBI CCWGG 1 cut(s) 509
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 3 cut(s) 234, 507, 589
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 2 cut(s) 852, 1109
BseLI CCNNNNNNNGG 5 cut(s) 162, 236, 292, 719, 994
BseMI GCAATG 1 cut(s) 1077
BseMII CTCAG 2 cut(s) 270, 879
BseNI ACTGG 1 cut(s) 343
BseXI GCAGC 1 cut(s) 565
Bsh1236I CGCG 2 cut(s) 630, 758
BshFI GGCC 3 cut(s) 477, 795, 1099
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 2 cut(s) 116, 315
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 5 cut(s) 162, 236, 292, 719, 994
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 3 cut(s) 477, 795, 1099
Bsp1286I GDGCHC 1 cut(s) 285
Bsp13I TCCGGA 1 cut(s) 314
Bsp143I GATC 5 cut(s) 147, 183, 811, 982, 1006
BspACI CCGC 5 cut(s) 199, 474, 630, 758, 1037
BspANI GGCC 3 cut(s) 477, 795, 1099
BspCNI CTCAG 2 cut(s) 271, 880
BspDI ATCGAT 1 cut(s) 150
BspEI TCCGGA 1 cut(s) 314
BspFNI CGCG 2 cut(s) 630, 758
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 2 cut(s) 348, 513
BspPI GGATC 3 cut(s) 191, 819, 977
BspTI CTTAAG 1 cut(s) 976
BsrBI CCGCTC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 1077
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 3 cut(s) 234, 507, 589
BssMI GATC 5 cut(s) 147, 183, 811, 982, 1006
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 224, 355, 865
Bst6I CTCTTC 1 cut(s) 1037
BstAFI CTTAAG 1 cut(s) 976
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstBAI YACGTR 1 cut(s) 674
BstC8I GCNNGC 3 cut(s) 231, 383, 1062
BstDEI CTNAG 5 cut(s) 279, 479, 888, 925, 989
BstENI CCTNNNNNAGG 2 cut(s) 160, 992
BstF5I GGATG 1 cut(s) 22
BstFNI CGCG 2 cut(s) 630, 758
BstHHI GCGC 2 cut(s) 48, 630
BstKTI GATC 5 cut(s) 150, 186, 814, 985, 1009
BstMBI GATC 5 cut(s) 147, 183, 811, 982, 1006
BstMWI GCNNNNNNNGC 3 cut(s) 547, 1061, 1088
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 1 cut(s) 1064
BstSCI CCNGG 1 cut(s) 507
BstUI CGCG 2 cut(s) 630, 758
BstV1I GCAGC 1 cut(s) 565
BstV2I GAAGAC 2 cut(s) 378, 416
BstX2I RGATCY 2 cut(s) 183, 982
BstYI RGATCY 2 cut(s) 183, 982
Bsu15I ATCGAT 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 989
BsuRI GGCC 3 cut(s) 477, 795, 1099
BsuTUI ATCGAT 1 cut(s) 150
BtgZI GCGATG 2 cut(s) 226, 1041
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 3 cut(s) 231, 383, 1062
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 2 cut(s) 48, 630
Cfr13I GGNCC 2 cut(s) 511, 959
ClaI ATCGAT 1 cut(s) 150
CseI GACGC 1 cut(s) 8
Csp6I GTAC 2 cut(s) 749, 861
CviAII CATG 4 cut(s) 551, 709, 1010, 1061
CviQI GTAC 2 cut(s) 749, 861
DdeI CTNAG 5 cut(s) 279, 479, 888, 925, 989
DpnI GATC 5 cut(s) 149, 185, 813, 984, 1008
DpnII GATC 5 cut(s) 147, 183, 811, 982, 1006
DrdI GACNNNNNNGTC 1 cut(s) 509
DseDI GACNNNNNNGTC 1 cut(s) 509
Eam1104I CTCTTC 1 cut(s) 1037
EarI CTCTTC 1 cut(s) 1037
Ecl136II GAGCTC 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 795
Eco24I GRGCYC 1 cut(s) 285
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 2 cut(s) 511, 959
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 2 cut(s) 791, 948
Eco72I CACGTG 1 cut(s) 674
Eco81I CCTNAGG 1 cut(s) 989
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 2 cut(s) 160, 992
EcoRII CCWGG 1 cut(s) 507
EcoRV GATATC 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 285
FaeI CATG 4 cut(s) 554, 712, 1013, 1064
FaqI GGGAC 1 cut(s) 497
FatI CATG 4 cut(s) 550, 708, 1009, 1060
FauI CCCGC 1 cut(s) 623
FbaI TGATCA 1 cut(s) 1006
Fnu4HI GCNGC 2 cut(s) 475, 579
FokI GGATG 1 cut(s) 9
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 2 cut(s) 475, 579
FspBI CTAG 2 cut(s) 416, 1016
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 47, 629
GluI GCNGC 2 cut(s) 475, 579
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 3 cut(s) 477, 795, 1099
HapII CCGG 2 cut(s) 116, 315
HgaI GACGC 1 cut(s) 8
HhaI GCGC 2 cut(s) 48, 630
Hin1II CATG 4 cut(s) 554, 712, 1013, 1064
Hin6I GCGC 2 cut(s) 46, 628
HinP1I GCGC 2 cut(s) 46, 628
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 5 cut(s) 152, 328, 566, 696, 921
HpaII CCGG 2 cut(s) 116, 315
HphI GGTGA 2 cut(s) 806, 1051
Hpy188I TCNGA 3 cut(s) 161, 534, 967
Hpy188III TCNNGA 4 cut(s) 315, 809, 841, 1010
HpyAV CCTTC 5 cut(s) 156, 166, 296, 394, 972
HpyCH4III ACNGT 3 cut(s) 224, 355, 865
HpyCH4IV ACGT 2 cut(s) 673, 690
HpyCH4V TGCA 7 cut(s) 59, 204, 385, 446, 518, 909, 1082
HpyF10VI GCNNNNNNNGC 3 cut(s) 547, 1061, 1088
HpyF3I CTNAG 5 cut(s) 279, 479, 888, 925, 989
HpySE526I ACGT 2 cut(s) 673, 690
Hsp92II CATG 4 cut(s) 554, 712, 1013, 1064
HspAI GCGC 2 cut(s) 46, 628
Kpn2I TCCGGA 1 cut(s) 314
Ksp22I TGATCA 1 cut(s) 1006
Kzo9I GATC 5 cut(s) 147, 183, 811, 982, 1006
LmnI GCTCC 4 cut(s) 288, 317, 346, 664
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 2 cut(s) 175, 1064
MaeI CTAG 2 cut(s) 416, 1016
MaeII ACGT 2 cut(s) 673, 690
MaeIII GTNAC 2 cut(s) 421, 593
MalI GATC 5 cut(s) 149, 185, 813, 984, 1008
MbiI CCGCTC 1 cut(s) 199
MboI GATC 5 cut(s) 147, 183, 811, 982, 1006
MboII GAAGA 4 cut(s) 383, 421, 1054, 1060
MflI RGATCY 2 cut(s) 183, 982
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 4 cut(s) 613, 684, 835, 1023
MlyI GAGTC 2 cut(s) 560, 915
MmeI TCCRAC 1 cut(s) 747
MnlI CCTC 9 cut(s) 112, 174, 229, 257, 335, 351, 599, 961, 1038
MroI TCCGGA 1 cut(s) 314
MroXI GAANNNNTTC 1 cut(s) 97
MseI TTAA 6 cut(s) 29, 573, 606, 687, 738, 977
MslI CAYNNNNRTG 2 cut(s) 897, 1113
MspA1I CMGCKG 1 cut(s) 1037
MspCI CTTAAG 1 cut(s) 976
MspI CCGG 2 cut(s) 116, 315
MspR9I CCNGG 1 cut(s) 509
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 2 cut(s) 630, 758
MwoI GCNNNNNNNGC 3 cut(s) 547, 1061, 1088
NdeII GATC 5 cut(s) 147, 183, 811, 982, 1006
NlaIII CATG 4 cut(s) 554, 712, 1013, 1064
NlaIV GGNNCC 2 cut(s) 348, 513
NmeAIII GCCGAG 1 cut(s) 259
NmuCI GTSAC 1 cut(s) 421
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 1 cut(s) 1064
PaeI GCATGC 1 cut(s) 1064
PagI TCATGA 1 cut(s) 1009
PceI AGGCCT 1 cut(s) 795
PctI GAATGC 1 cut(s) 476
PdmI GAANNNNTTC 1 cut(s) 97
PfeI GAWTC 3 cut(s) 152, 328, 696
PkrI GCNGC 2 cut(s) 476, 580
PleI GAGTC 2 cut(s) 560, 915
PmaCI CACGTG 1 cut(s) 674
PmlI CACGTG 1 cut(s) 674
PpsI GAGTC 2 cut(s) 560, 915
Ppu21I YACGTR 1 cut(s) 674
PshBI ATTAAT 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 285
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 507
PspCI CACGTG 1 cut(s) 674
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 2 cut(s) 348, 513
PspPI GGNCC 2 cut(s) 511, 959
PsuI RGATCY 2 cut(s) 183, 982
RsaI GTAC 2 cut(s) 750, 862
RsaNI GTAC 2 cut(s) 749, 861
RseI CAYNNNNRTG 2 cut(s) 897, 1113
SacI GAGCTC 1 cut(s) 285
SaqAI TTAA 6 cut(s) 29, 573, 606, 687, 738, 977
SatI GCNGC 2 cut(s) 475, 579
Sau3AI GATC 5 cut(s) 147, 183, 811, 982, 1006
Sau96I GGNCC 2 cut(s) 511, 959
SchI GAGTC 2 cut(s) 560, 915
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 1 cut(s) 285
SfaNI GCATC 2 cut(s) 175, 1064
SinI GGWCC 2 cut(s) 511, 959
SmiMI CAYNNNNRTG 2 cut(s) 897, 1113
SmlI CTYRAG 2 cut(s) 178, 976
SmoI CTYRAG 2 cut(s) 178, 976
SphI GCATGC 1 cut(s) 1064
Sse9I AATT 4 cut(s) 613, 684, 835, 1023
SseBI AGGCCT 1 cut(s) 795
SsiI CCGC 5 cut(s) 199, 474, 630, 758, 1037
SspI AATATT 1 cut(s) 493
SspMI CTAG 2 cut(s) 416, 1016
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 3 cut(s) 224, 355, 865
TaiI ACGT 2 cut(s) 676, 693
TaqI TCGA 6 cut(s) 137, 150, 289, 369, 569, 694
TasI AATT 4 cut(s) 613, 684, 835, 1023
TauI GCSGC 1 cut(s) 477
TfiI GAWTC 3 cut(s) 152, 328, 696
Tru1I TTAA 6 cut(s) 29, 573, 606, 687, 738, 977
Tru9I TTAA 6 cut(s) 29, 573, 606, 687, 738, 977
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 1 cut(s) 421
TseI GCWGC 1 cut(s) 578
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 5 cut(s) 29, 567, 688, 697, 1026
TspRI CASTG 1 cut(s) 283
Vha464I CTTAAG 1 cut(s) 976
VpaK11BI GGWCC 2 cut(s) 511, 959
VspI ATTAAT 1 cut(s) 573
XagI CCTNNNNNAGG 2 cut(s) 160, 992
XapI RAATTY 1 cut(s) 835
XceI RCATGY 1 cut(s) 1064
XmnI GAANNNNTTC 1 cut(s) 97
XspI CTAG 2 cut(s) 416, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.