Rw1G000110

Cyclic pyranopterin monophosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002342
Physical Location & Seq
Reverse (-)
153290 .. 158172
4883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000110.1

Sequence Viewer

Length: 873 bp
ATGTTGAAGCGCCAACTATCAAAGCTTCCCCACTTACGTACAGGCTTTGGAAATTTCAATTCTTTCCTGTGCTCATGCTTTGAAACTGGGTCCACCAGTGGTGACGCCAAGGGCTTACTTCATCAGCAAATGCGAATGAATGGCTCCCCTTACGCAACTTGTTGTGCCAAGTTGCCGGAGGAGCTTCCAAAAGATAATCCTGTTTCCAATATGTTGATTGATTCTTTTGGAAGGCAGCACACTTACTTGAGGATCTCTTTAACAGAGCGTTGCAATCTGCGATGTCAATACTGCATGCCGGCCGAGGGTGTCAGCCTCACTCCTGGCCCTCAAATTCTCTCGCAGAATGAGCTTGTTCGCTTGGCTAATCTGTTTGTAAGCTCCGGAGTCAATAAAATTCGTTTGACTGGAGGGGAGCCAACCGTCAGGAAAGATATTGAAGACATATGCTTGCACTTGTCTAACTTGAAGGGACTGAAAACACTGGCTATCACTACTAATGGAATCACTCTTGCACGAAAACTTCCCAAGTTGAAAGAATGCGGCCTTAGTGCGGTGAATATTAGTTTAGACACCTTGGTCCCTGCAAAGTTTGAATTTCTGACCAGGCGTAGAGGGCATGAAAGGGTTATGGACTCAATTAATGCTGCCATAGACCTCGGTTATAATCCTGTTAAGGTAAATTGCGTTGTAATGCGTGGGTTCAATGATGATGAGATTTGCAATTTCGTAGAGCTGACACGTGAAAAGCCAATTAACATTCGATTCATTGAGTTCATGCCTTTTGATGGAAATGTATGGAATGTTAAGAAACTTGTACCCTACGCAGAAATGTTGGATAGAGTGGTAAGGCAAAACATTTTACAGGCCTAA

Protein Analysis

290

Amino Acids

32.56

Weight (kDa)

9.05

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 85 - 246 1.1e-34 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AasI GACNNNNNNGTC 1 cut(s) 578
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 2 cut(s) 543, 554
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 4 cut(s) 52, 333, 396, 596
AcvI CACGTG 1 cut(s) 743
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 2 cut(s) 40, 819
AfiI CCNNNNNNNGG 3 cut(s) 305, 553, 788
AflIII ACRYGT 1 cut(s) 740
AgsI TTSAA 8 cut(s) 7, 58, 83, 440, 469, 535, 596, 706
AjnI CCWGG 2 cut(s) 322, 605
AluBI AGCT 5 cut(s) 25, 184, 352, 381, 736
AluI AGCT 5 cut(s) 25, 184, 352, 381, 736
Alw21I GWGCWC 1 cut(s) 74
AlwI GGATC 1 cut(s) 260
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 4 cut(s) 300, 325, 544, 867
ApeKI GCWGC 2 cut(s) 235, 647
ApoI RAATTY 4 cut(s) 52, 333, 396, 596
AseI ATTAAT 1 cut(s) 642
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 3 cut(s) 90, 326, 580
AsuHPI GGTGA 2 cut(s) 113, 568
AvaII GGWCC 2 cut(s) 90, 580
BbrPI CACGTG 1 cut(s) 743
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 2 cut(s) 247, 634
BccI CCATC 1 cut(s) 782
BciT130I CCWGG 2 cut(s) 324, 607
BfoI RGCGCY 1 cut(s) 13
BisI GCNGC 3 cut(s) 236, 544, 648
BlsI GCNGC 3 cut(s) 237, 545, 649
Bme1390I CCNGG 2 cut(s) 324, 607
Bme18I GGWCC 2 cut(s) 90, 580
BmgT120I GGNCC 3 cut(s) 90, 326, 580
BmiI GGNNCC 4 cut(s) 91, 145, 417, 582
BmrFI CCNGG 2 cut(s) 324, 607
BmrI ACTGGG 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 447
BpmI CTGGAG 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 268
BsaAI YACGTR 2 cut(s) 38, 743
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 4 cut(s) 108, 303, 576, 658
BsaWI WCCGGW 1 cut(s) 383
Bsc4I CCNNNNNNNGG 3 cut(s) 305, 553, 788
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 4 cut(s) 91, 96, 412, 489
BseAI TCCGGA 1 cut(s) 383
BseBI CCWGG 2 cut(s) 324, 607
BseDI CCNNGG 4 cut(s) 108, 303, 576, 658
BseLI CCNNNNNNNGG 3 cut(s) 305, 553, 788
BseNI ACTGG 4 cut(s) 91, 96, 412, 489
BseRI GAGGAG 1 cut(s) 194
BseX3I CGGCCG 1 cut(s) 300
BseXI GCAGC 2 cut(s) 247, 634
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 4 cut(s) 302, 327, 546, 869
BsiEI CGRYCG 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 3 cut(s) 176, 299, 384
BslFI GGGAC 2 cut(s) 486, 566
BslI CCNNNNNNNGG 3 cut(s) 305, 553, 788
BsmFI GGGAC 2 cut(s) 486, 566
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 4 cut(s) 302, 327, 546, 869
Bsp1286I GDGCHC 1 cut(s) 74
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 1 cut(s) 252
BspACI CCGC 2 cut(s) 543, 554
BspANI GGCC 4 cut(s) 302, 327, 546, 869
BspEI TCCGGA 1 cut(s) 383
BspLI GGNNCC 4 cut(s) 91, 145, 417, 582
BspPI GGATC 1 cut(s) 260
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 4 cut(s) 91, 96, 412, 489
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 4 cut(s) 108, 303, 576, 658
BssMI GATC 1 cut(s) 252
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 2 cut(s) 108, 576
Bst2UI CCWGG 2 cut(s) 324, 607
Bst4CI ACNGT 1 cut(s) 424
BstACI GRCGYC 1 cut(s) 105
BstBAI YACGTR 2 cut(s) 38, 743
BstC8I GCNNGC 3 cut(s) 296, 300, 452
BstDEI CTNAG 1 cut(s) 548
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 1 cut(s) 255
BstMBI GATC 1 cut(s) 252
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 3 cut(s) 181, 349, 616
BstNI CCWGG 2 cut(s) 324, 607
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 2 cut(s) 322, 605
BstSNI TACGTA 1 cut(s) 38
BstV1I GCAGC 2 cut(s) 247, 634
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BstZI CGGCCG 1 cut(s) 300
BsuRI GGCC 4 cut(s) 302, 327, 546, 869
BtgZI GCGATG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 103, 482
Cac8I GCNNGC 3 cut(s) 296, 300, 452
CfoI GCGC 1 cut(s) 12
Cfr10I RCCGGY 1 cut(s) 298
Cfr13I GGNCC 3 cut(s) 90, 326, 580
CseI GACGC 1 cut(s) 113
Csp6I GTAC 2 cut(s) 39, 818
CviAII CATG 4 cut(s) 75, 295, 620, 778
CviQI GTAC 2 cut(s) 39, 818
DdeI CTNAG 1 cut(s) 548
DpnI GATC 1 cut(s) 254
DpnII GATC 1 cut(s) 252
DrdI GACNNNNNNGTC 1 cut(s) 578
DseDI GACNNNNNNGTC 1 cut(s) 578
EaeI YGGCCR 1 cut(s) 300
EagI CGGCCG 1 cut(s) 300
EclXI CGGCCG 1 cut(s) 300
Eco105I TACGTA 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 108, 576
Eco147I AGGCCT 1 cut(s) 869
Eco47I GGWCC 2 cut(s) 90, 580
Eco52I CGGCCG 1 cut(s) 300
Eco72I CACGTG 1 cut(s) 743
EcoRII CCWGG 2 cut(s) 322, 605
EcoT14I CCWWGG 2 cut(s) 108, 576
ErhI CCWWGG 2 cut(s) 108, 576
FaeI CATG 4 cut(s) 78, 298, 623, 781
FaqI GGGAC 2 cut(s) 486, 566
FatI CATG 4 cut(s) 74, 294, 619, 777
FauNDI CATATG 1 cut(s) 446
Fnu4HI GCNGC 3 cut(s) 236, 544, 648
Fsp4HI GCNGC 3 cut(s) 236, 544, 648
GlaI GCGC 1 cut(s) 11
GluI GCNGC 3 cut(s) 236, 544, 648
GsuI CTGGAG 1 cut(s) 429
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 4 cut(s) 302, 327, 546, 869
HapII CCGG 3 cut(s) 176, 299, 384
HgaI GACGC 1 cut(s) 113
HhaI GCGC 1 cut(s) 12
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 4 cut(s) 78, 298, 623, 781
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 5 cut(s) 221, 387, 504, 635, 765
HpaII CCGG 3 cut(s) 176, 299, 384
HphI GGTGA 2 cut(s) 113, 568
Hpy166II GTNNAC 1 cut(s) 93
Hpy188I TCNGA 1 cut(s) 603
Hpy188III TCNNGA 2 cut(s) 384, 427
Hpy8I GTNNAC 1 cut(s) 93
HpyAV CCTTC 2 cut(s) 225, 463
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 2 cut(s) 37, 742
HpyCH4V TGCA 6 cut(s) 273, 294, 454, 515, 587, 723
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 349, 616
HpyF3I CTNAG 1 cut(s) 548
HpySE526I ACGT 2 cut(s) 37, 742
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 4 cut(s) 78, 298, 623, 781
HspAI GCGC 1 cut(s) 10
Kpn2I TCCGGA 1 cut(s) 383
KroI GCCGGC 1 cut(s) 298
KroNI GCCGGC 1 cut(s) 300
Kzo9I GATC 1 cut(s) 252
LmnI GCTCC 4 cut(s) 149, 181, 386, 415
Lsp1109I GCAGC 2 cut(s) 247, 634
MaeII ACGT 2 cut(s) 37, 742
MaeIII GTNAC 1 cut(s) 101
MalI GATC 1 cut(s) 254
MboI GATC 1 cut(s) 252
MboII GAAGA 1 cut(s) 452
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 9 cut(s) 52, 58, 333, 396, 596, 639, 682, 724, 753
MlyI GAGTC 2 cut(s) 396, 629
MmeI TCCRAC 1 cut(s) 816
MnlI CCTC 8 cut(s) 172, 243, 298, 326, 339, 404, 608, 668
MroI TCCGGA 1 cut(s) 383
MroNI GCCGGC 1 cut(s) 298
MseI TTAA 5 cut(s) 260, 642, 675, 756, 807
MspI CCGG 3 cut(s) 176, 299, 384
MspR9I CCNGG 2 cut(s) 324, 607
Mva1269I GAATGC 1 cut(s) 545
MvaI CCWGG 2 cut(s) 324, 607
MwoI GCNNNNNNNGC 3 cut(s) 181, 349, 616
NaeI GCCGGC 1 cut(s) 300
NdeI CATATG 1 cut(s) 446
NdeII GATC 1 cut(s) 252
NgoMIV GCCGGC 1 cut(s) 298
NlaIII CATG 4 cut(s) 78, 298, 623, 781
NlaIV GGNNCC 4 cut(s) 91, 145, 417, 582
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 1 cut(s) 101
NspI RCATGY 1 cut(s) 298
PaeI GCATGC 1 cut(s) 298
PceI AGGCCT 1 cut(s) 869
PctI GAATGC 1 cut(s) 545
PdiI GCCGGC 1 cut(s) 300
PfeI GAWTC 3 cut(s) 221, 504, 765
PkrI GCNGC 3 cut(s) 237, 545, 649
PleI GAGTC 2 cut(s) 395, 629
PmaCI CACGTG 1 cut(s) 743
PmlI CACGTG 1 cut(s) 743
PpsI GAGTC 2 cut(s) 395, 629
Ppu21I YACGTR 2 cut(s) 38, 743
PshBI ATTAAT 1 cut(s) 642
PsiI TTATAA 1 cut(s) 666
Psp6I CCWGG 2 cut(s) 322, 605
PspCI CACGTG 1 cut(s) 743
PspGI CCWGG 2 cut(s) 322, 605
PspN4I GGNNCC 4 cut(s) 91, 145, 417, 582
PspPI GGNCC 3 cut(s) 90, 326, 580
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 2 cut(s) 40, 819
RsaNI GTAC 2 cut(s) 39, 818
SaqAI TTAA 5 cut(s) 260, 642, 675, 756, 807
SatI GCNGC 3 cut(s) 236, 544, 648
Sau3AI GATC 1 cut(s) 252
Sau96I GGNCC 3 cut(s) 90, 326, 580
SchI GAGTC 2 cut(s) 396, 629
ScrFI CCNGG 2 cut(s) 324, 607
SduI GDGCHC 1 cut(s) 74
SinI GGWCC 2 cut(s) 90, 580
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
SnaBI TACGTA 1 cut(s) 38
SphI GCATGC 1 cut(s) 298
Sse9I AATT 9 cut(s) 52, 58, 333, 396, 596, 639, 682, 724, 753
SseBI AGGCCT 1 cut(s) 869
SsiI CCGC 2 cut(s) 543, 554
SspI AATATT 1 cut(s) 562
StuI AGGCCT 1 cut(s) 869
StyD4I CCNGG 2 cut(s) 322, 605
StyI CCWWGG 2 cut(s) 108, 576
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 2 cut(s) 40, 745
TaqI TCGA 1 cut(s) 763
TasI AATT 9 cut(s) 52, 58, 333, 396, 596, 639, 682, 724, 753
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 3 cut(s) 221, 504, 765
Tru1I TTAA 5 cut(s) 260, 642, 675, 756, 807
Tru9I TTAA 5 cut(s) 260, 642, 675, 756, 807
TscAI CASTG 2 cut(s) 103, 489
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 2 cut(s) 235, 647
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 5 cut(s) 110, 152, 636, 757, 766
TspRI CASTG 2 cut(s) 103, 489
VpaK11BI GGWCC 2 cut(s) 90, 580
VspI ATTAAT 1 cut(s) 642
XapI RAATTY 4 cut(s) 52, 333, 396, 596
XceI RCATGY 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.