FvH4_5g32260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006189
Physical Location & Seq
Forward (+)
23271635 .. 23275369
3735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32260.t1

Sequence Viewer

Length: 1521 bp
ATGCTTAATTTGTTTCTGTCGGAGCCCAATTGGAAAGATGATGACTCTGCTGAAATGAGAATGTCTCTCTTGAACAAATTGGAATCTGTCATTTGGTCGTTGATGGCTTCTGGAGGTCGATCAGAGGCTAGACTATGGCTTTGCAACACTATAGCTGGCATAACTTCTATCACTCCTAATCATCAACGTGAGTTATTTATCGACTTATTGAGATCTAAGCCACTGAAGAAGCGCTTAGCAACTCAGCTTCTGCACATGATATTTGATAATAGGCCACAAAGAGCAGGGTCTATCATAGCCAAGAGAAGTTATGTGCTTAGGAAATTTTTCAAAGGAAACCCAACACGCATATCTCAGTGGTTTTCTAAAACTGTTTCTGGATTGGGGCAGGGACAAGGTGCACTATCCCAATTTGCTTTTGTTAATCGTGATAATTGTTGGGAGGAGCTTGAGTGGAAGGGAAAACATGGGCAGTCACCGGCAGTAGTTGCTACAAAACCCCATTACTTTCTCGATTTGGATGTGCAGCAAACTGTAGAGAATTTCCTTGAAAATGTGCCTGAATTTTGGTCTTCCAATGAGTTTTCTGAGTCACTTAAAGGTGGTGAAATTTTGTTCATTGATAGGAACTTTTTTGTTCTATATTTTCTGGATTTGATGTACAAAGAAGATGCCAGAGATGTATGGGAGGTTACAAATGAGTTCCTGGAGGAGGAGCAATTTTCTTCCTTGTGTAAACACCTTCTTATTACTCTTGAGGAGTCGGACTTATGTGAGTTTCTTAAAATGCTTCATAAACATGTCAAGCCTAGAGTGGAATGCAAAGATCCTATGGACTCCTCTTATTTGTTTGAAGTTGTAGTATCTAAGTGCAGAGATTGTGGGTGCTTTGATCAGATATTACTGTTAAATGCAGTTTTCAACTATGGACGCCAGCTTCTACGGCTTTTACGAGATGAAGGAGCGCCTGAGGATCAAGCAAAACTACAAGATATTGTGTACGAGATTTGTGCAATCCCAGGCAATGAAAACAGCATGGCCCCAATAATTAAAGGGGGATCTAAGCTGAAGATTATAGAAGCATTTAAATTAATGGGGCTTCAGTCATGGGTTCTCTTTTATAGTTTATCAGAAAAGTGTCAGACTCTTCAGTCTTGGGAATCATTATTTATTGATAATGCAATAAGCTTTCGAAAGTACGATAAATATTCATTTCTTCATGGTAATGAAGCTGCTGAAGAAGGGGGTTCTGATTTGGATTACAGAGCATCCAAAAAAATTAAGCAAAGGAAAAAGAGTAGTAGAAGGAAAAGAAGAAGGGACTTTGATTATGATGACAATGAGTTGCTGGATTTTGATACTACAACTATTAGGCTGGATGTGCATTCCACTGCCGGAAGTTGGTTGCTCTCACTCGATGGGTATTCTGCATCATGGAACAGCGCGGATTTACCTGAATATCTTTCCAAGTATTGCTTGTCGAGATGGATAAAGTGGACTGTAGAACAGGGGGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

507

Amino Acids

58.69

Weight (kDa)

5.86

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1150
AccII CGCG 1 cut(s) 1445
AciI CCGC 1 cut(s) 1445
AclWI GGATC 3 cut(s) 821, 981, 1066
AcsI RAATTY 4 cut(s) 323, 541, 563, 609
AcuI CTGAAG 5 cut(s) 245, 1085, 1088, 1133, 1257
AcyI GRCGYC 1 cut(s) 931
AfaI GTAC 3 cut(s) 662, 1001, 1199
AfeI AGCGCT 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 712, 1401
AflIII ACRYGT 1 cut(s) 799
AgsI TTSAA 5 cut(s) 73, 331, 551, 854, 922
AjnI CCWGG 2 cut(s) 705, 1018
AluBI AGCT 7 cut(s) 155, 247, 448, 937, 1066, 1188, 1232
AluI AGCT 7 cut(s) 155, 247, 448, 937, 1066, 1188, 1232
Alw21I GWGCWC 1 cut(s) 403
Alw26I GTCTC 1 cut(s) 69
Alw44I GTGCAC 1 cut(s) 399
AlwI GGATC 3 cut(s) 821, 981, 1066
AlwNI CAGNNNCTG 1 cut(s) 250
Aor51HI AGCGCT 1 cut(s) 233
AoxI GGCC 2 cut(s) 272, 1038
ApaLI GTGCAC 1 cut(s) 399
ApeKI GCWGC 2 cut(s) 526, 1232
ApoI RAATTY 4 cut(s) 323, 541, 563, 609
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
AseI ATTAAT 1 cut(s) 1091
Asp700I GAANNNNTTC 1 cut(s) 326
AspLEI GCGC 3 cut(s) 234, 967, 1445
AspS9I GGNCC 1 cut(s) 1039
AsuHPI GGTGA 2 cut(s) 468, 617
AsuII TTCGAA 1 cut(s) 1192
AxyI CCTNAGG 1 cut(s) 969
BaeGI GKGCMC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 2 cut(s) 538, 1219
BccI CCATC 3 cut(s) 97, 1412, 1479
BceAI ACGGC 1 cut(s) 959
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BciT130I CCWGG 2 cut(s) 707, 1020
BclI TGATCA 1 cut(s) 892
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 2 cut(s) 129, 810
BfmI CTRYAG 3 cut(s) 150, 534, 1500
BfoI RGCGCY 2 cut(s) 235, 968
BglII AGATCT 1 cut(s) 212
BisI GCNGC 2 cut(s) 527, 1233
BlpI GCTNAGC 1 cut(s) 235
BlsI GCNGC 2 cut(s) 528, 1234
Bme1390I CCNGG 2 cut(s) 707, 1020
BmgT120I GGNCC 1 cut(s) 1039
BmiI GGNNCC 2 cut(s) 24, 1041
BmrFI CCNGG 2 cut(s) 707, 1020
BmsI GCATC 3 cut(s) 661, 1277, 1439
BpiI GAAGAC 1 cut(s) 564
BplI GAGNNNNNCTC 2 cut(s) 49, 81
BpmI CTGGAG 2 cut(s) 132, 728
Bpu10I CCTNAGC 1 cut(s) 317
Bpu1102I GCTNAGC 1 cut(s) 235
Bpu14I TTCGAA 1 cut(s) 1192
BpuEI CTTGAG 2 cut(s) 470, 776
BsaHI GRCGYC 1 cut(s) 931
BsaJI CCNNGG 1 cut(s) 1018
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 712, 1401
Bse118I RCCGGY 1 cut(s) 478
Bse21I CCTNAGG 1 cut(s) 969
Bse3DI GCAATG 1 cut(s) 1030
BseBI CCWGG 2 cut(s) 707, 1020
BseDI CCNNGG 1 cut(s) 1018
BseGI GGATG 3 cut(s) 526, 1268, 1384
BseLI CCNNNNNNNGG 2 cut(s) 712, 1401
BseMI GCAATG 1 cut(s) 1030
BseMII CTCAG 4 cut(s) 257, 368, 579, 960
BseRI GAGGAG 5 cut(s) 458, 725, 728, 773, 829
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 2 cut(s) 538, 1219
BsgI GTGCAG 3 cut(s) 236, 545, 892
Bsh1236I CGCG 1 cut(s) 1445
BshFI GGCC 2 cut(s) 274, 1040
BsiHKAI GWGCWC 1 cut(s) 403
BsiSI CCGG 2 cut(s) 479, 1395
BslFI GGGAC 2 cut(s) 405, 1334
BslI CCNNNNNNNGG 2 cut(s) 712, 1401
BsmAI GTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 405, 1334
BsmI GAATGC 2 cut(s) 824, 1384
BsnI GGCC 2 cut(s) 274, 1040
Bsp119I TTCGAA 1 cut(s) 1192
Bsp1286I GDGCHC 2 cut(s) 27, 403
Bsp1407I TGTACA 1 cut(s) 660
Bsp143I GATC 6 cut(s) 119, 212, 826, 892, 973, 1058
Bsp1720I GCTNAGC 1 cut(s) 235
BspACI CCGC 1 cut(s) 1445
BspANI GGCC 2 cut(s) 274, 1040
BspCNI CTCAG 4 cut(s) 256, 367, 580, 961
BspFNI CGCG 1 cut(s) 1445
BspLI GGNNCC 2 cut(s) 24, 1041
BspPI GGATC 3 cut(s) 821, 981, 1066
BspT104I TTCGAA 1 cut(s) 1192
BsrDI GCAATG 1 cut(s) 1030
BsrFI RCCGGY 1 cut(s) 478
BsrGI TGTACA 1 cut(s) 660
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 1018
BssMI GATC 6 cut(s) 119, 212, 826, 892, 973, 1058
BssNI GRCGYC 1 cut(s) 931
Bst2UI CCWGG 2 cut(s) 707, 1020
Bst4CI ACNGT 4 cut(s) 373, 535, 906, 1501
Bst6I CTCTTC 1 cut(s) 1152
BstACI GRCGYC 1 cut(s) 931
BstAPI GCANNNNNTGC 1 cut(s) 488
BstAUI TGTACA 1 cut(s) 660
BstBI TTCGAA 1 cut(s) 1192
BstC8I GCNNGC 2 cut(s) 157, 935
BstDEI CTNAG 9 cut(s) 216, 235, 243, 317, 354, 588, 867, 969, 1062
BstENI CCTNNNNNAGG 1 cut(s) 710
BstF5I GGATG 3 cut(s) 526, 1268, 1384
BstFNI CGCG 1 cut(s) 1445
BstH2I RGCGCY 2 cut(s) 235, 968
BstHHI GCGC 3 cut(s) 234, 967, 1445
BstKTI GATC 6 cut(s) 122, 215, 829, 895, 976, 1061
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 6 cut(s) 119, 212, 826, 892, 973, 1058
BstMWI GCNNNNNNNGC 3 cut(s) 488, 943, 1381
BstNI CCWGG 2 cut(s) 707, 1020
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 2 cut(s) 705, 1018
BstSFI CTRYAG 3 cut(s) 150, 534, 1500
BstSLI GKGCMC 1 cut(s) 403
BstUI CGCG 1 cut(s) 1445
BstV1I GCAGC 2 cut(s) 538, 1219
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 3 cut(s) 212, 826, 1058
BstYI RGATCY 3 cut(s) 212, 826, 1058
Bsu36I CCTNAGG 1 cut(s) 969
BsuRI GGCC 2 cut(s) 274, 1040
BtsCI GGATG 3 cut(s) 526, 1268, 1384
BtsI GCAGTG 1 cut(s) 1389
BtsIMutI CAGTG 3 cut(s) 221, 362, 1389
Cac8I GCNNGC 2 cut(s) 157, 935
CaiI CAGNNNCTG 1 cut(s) 250
CfoI GCGC 3 cut(s) 234, 967, 1445
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 1039
CseI GACGC 1 cut(s) 939
Csp6I GTAC 3 cut(s) 661, 1000, 1198
CviAII CATG 7 cut(s) 256, 467, 800, 1036, 1107, 1220, 1434
CviQI GTAC 3 cut(s) 661, 1000, 1198
DdeI CTNAG 9 cut(s) 216, 235, 243, 317, 354, 588, 867, 969, 1062
DpnI GATC 6 cut(s) 121, 214, 828, 894, 975, 1060
DpnII GATC 6 cut(s) 119, 212, 826, 892, 973, 1058
DraI TTTAAA 1 cut(s) 1087
DrdI GACNNNNNNGTC 1 cut(s) 1150
DseDI GACNNNNNNGTC 1 cut(s) 1150
Eam1104I CTCTTC 1 cut(s) 1152
EarI CTCTTC 1 cut(s) 1152
Eco24I GRGCYC 1 cut(s) 27
Eco47III AGCGCT 1 cut(s) 233
Eco57I CTGAAG 5 cut(s) 245, 1085, 1088, 1133, 1257
Eco81I CCTNAGG 1 cut(s) 969
EcoNI CCTNNNNNAGG 1 cut(s) 710
EcoRII CCWGG 2 cut(s) 705, 1018
EcoT38I GRGCYC 1 cut(s) 27
FaeI CATG 7 cut(s) 259, 470, 803, 1039, 1110, 1223, 1437
FalI AAGNNNNNCTT 4 cut(s) 218, 250, 1460, 1492
FaqI GGGAC 2 cut(s) 405, 1334
FatI CATG 7 cut(s) 255, 466, 799, 1035, 1106, 1219, 1433
FbaI TGATCA 1 cut(s) 892
Fnu4HI GCNGC 2 cut(s) 527, 1233
FokI GGATG 3 cut(s) 533, 1255, 1391
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 2 cut(s) 527, 1233
FspBI CTAG 2 cut(s) 129, 810
GlaI GCGC 3 cut(s) 233, 966, 1444
GluI GCNGC 2 cut(s) 527, 1233
GsuI CTGGAG 2 cut(s) 132, 728
HaeII RGCGCY 2 cut(s) 235, 968
HaeIII GGCC 2 cut(s) 274, 1040
HapII CCGG 2 cut(s) 479, 1395
HgaI GACGC 1 cut(s) 939
HhaI GCGC 3 cut(s) 234, 967, 1445
Hin1I GRCGYC 1 cut(s) 931
Hin1II CATG 7 cut(s) 259, 470, 803, 1039, 1110, 1223, 1437
Hin6I GCGC 3 cut(s) 232, 965, 1443
HinP1I GCGC 3 cut(s) 232, 965, 1443
HindIII AAGCTT 1 cut(s) 1186
HinfI GANTC 7 cut(s) 44, 83, 590, 761, 836, 1144, 1160
HpaII CCGG 2 cut(s) 479, 1395
HphI GGTGA 2 cut(s) 468, 617
Hpy166II GTNNAC 4 cut(s) 401, 737, 1000, 1497
Hpy188I TCNGA 8 cut(s) 22, 124, 589, 766, 897, 1132, 1143, 1252
Hpy188III TCNNGA 8 cut(s) 70, 111, 378, 428, 512, 650, 755, 1482
Hpy8I GTNNAC 4 cut(s) 401, 737, 1000, 1497
HpyAV CCTTC 6 cut(s) 451, 752, 953, 1235, 1299, 1311
HpyCH4III ACNGT 4 cut(s) 373, 535, 906, 1501
HpyCH4IV ACGT 1 cut(s) 187
HpyF10VI GCNNNNNNNGC 3 cut(s) 488, 943, 1381
HpyF3I CTNAG 9 cut(s) 216, 235, 243, 317, 354, 588, 867, 969, 1062
HpySE526I ACGT 1 cut(s) 187
Hsp92I GRCGYC 1 cut(s) 931
Hsp92II CATG 7 cut(s) 259, 470, 803, 1039, 1110, 1223, 1437
HspAI GCGC 3 cut(s) 232, 965, 1443
Ksp22I TGATCA 1 cut(s) 892
Kzo9I GATC 6 cut(s) 119, 212, 826, 892, 973, 1058
LmnI GCTCC 4 cut(s) 22, 445, 715, 962
Lsp1109I GCAGC 2 cut(s) 538, 1219
LweI GCATC 3 cut(s) 661, 1277, 1439
MaeI CTAG 2 cut(s) 129, 810
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 3 cut(s) 474, 591, 691
MalI GATC 6 cut(s) 121, 214, 828, 894, 975, 1060
MboI GATC 6 cut(s) 119, 212, 826, 892, 973, 1058
MboII GAAGA 9 cut(s) 238, 564, 680, 717, 1081, 1139, 1208, 1250, 1326
MfeI CAATTG 1 cut(s) 28
MflI RGATCY 3 cut(s) 212, 826, 1058
MhlI GDGCHC 2 cut(s) 27, 403
MlyI GAGTC 5 cut(s) 38, 599, 770, 830, 1138
MmeI TCCRAC 1 cut(s) 744
MnlI CCTC 9 cut(s) 107, 118, 436, 682, 703, 706, 751, 850, 964
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 9 cut(s) 6, 423, 597, 783, 908, 1050, 1086, 1091, 1281
MslI CAYNNNNRTG 3 cut(s) 186, 798, 1224
MspI CCGG 2 cut(s) 479, 1395
MspR9I CCNGG 2 cut(s) 707, 1020
MunI CAATTG 1 cut(s) 28
Mva1269I GAATGC 2 cut(s) 824, 1384
MvaI CCWGG 2 cut(s) 707, 1020
MvnI CGCG 1 cut(s) 1445
MwoI GCNNNNNNNGC 3 cut(s) 488, 943, 1381
NdeII GATC 6 cut(s) 119, 212, 826, 892, 973, 1058
NlaIII CATG 7 cut(s) 259, 470, 803, 1039, 1110, 1223, 1437
NlaIV GGNNCC 2 cut(s) 24, 1041
NmuCI GTSAC 2 cut(s) 474, 591
NspI RCATGY 1 cut(s) 803
NspV TTCGAA 1 cut(s) 1192
PciI ACATGT 1 cut(s) 799
PcsI WCGNNNNNNNCGW 1 cut(s) 949
PctI GAATGC 2 cut(s) 824, 1384
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 2 cut(s) 83, 1160
PfoI TCCNGGA 1 cut(s) 705
PkrI GCNGC 2 cut(s) 528, 1234
PleI GAGTC 5 cut(s) 38, 598, 769, 830, 1138
PpsI GAGTC 5 cut(s) 38, 598, 769, 830, 1138
PscI ACATGT 1 cut(s) 799
PshBI ATTAAT 1 cut(s) 1091
Psp6I CCWGG 2 cut(s) 705, 1018
PspGI CCWGG 2 cut(s) 705, 1018
PspN4I GGNNCC 2 cut(s) 24, 1041
PspPI GGNCC 1 cut(s) 1039
PstNI CAGNNNCTG 1 cut(s) 250
PsuI RGATCY 3 cut(s) 212, 826, 1058
RsaI GTAC 3 cut(s) 662, 1001, 1199
RsaNI GTAC 3 cut(s) 661, 1000, 1198
RseI CAYNNNNRTG 3 cut(s) 186, 798, 1224
SaqAI TTAA 9 cut(s) 6, 423, 597, 783, 908, 1050, 1086, 1091, 1281
SatI GCNGC 2 cut(s) 527, 1233
Sau3AI GATC 6 cut(s) 119, 212, 826, 892, 973, 1058
Sau96I GGNCC 1 cut(s) 1039
SchI GAGTC 5 cut(s) 38, 599, 770, 830, 1138
ScrFI CCNGG 2 cut(s) 707, 1020
SduI GDGCHC 2 cut(s) 27, 403
SfaNI GCATC 3 cut(s) 661, 1277, 1439
SfcI CTRYAG 3 cut(s) 150, 534, 1500
SfuI TTCGAA 1 cut(s) 1192
SmiI ATTTAAAT 1 cut(s) 1087
SmiMI CAYNNNNRTG 3 cut(s) 186, 798, 1224
SmlI CTYRAG 2 cut(s) 449, 755
SmoI CTYRAG 2 cut(s) 449, 755
SsiI CCGC 1 cut(s) 1445
SspI AATATT 1 cut(s) 1208
SspMI CTAG 2 cut(s) 129, 810
StyD4I CCNGG 2 cut(s) 705, 1018
SwaI ATTTAAAT 1 cut(s) 1087
TaaI ACNGT 4 cut(s) 373, 535, 906, 1501
TaiI ACGT 1 cut(s) 190
TaqI TCGA 6 cut(s) 118, 201, 513, 1192, 1416, 1481
TatI WGTACW 1 cut(s) 660
TfiI GAWTC 2 cut(s) 83, 1160
Tru1I TTAA 9 cut(s) 6, 423, 597, 783, 908, 1050, 1086, 1091, 1281
Tru9I TTAA 9 cut(s) 6, 423, 597, 783, 908, 1050, 1086, 1091, 1281
TscAI CASTG 3 cut(s) 228, 362, 1396
TseFI GTSAC 2 cut(s) 474, 591
TseI GCWGC 2 cut(s) 526, 1232
Tsp45I GTSAC 2 cut(s) 474, 591
TspDTI ATGAA 7 cut(s) 607, 782, 972, 1041, 1200, 1208, 1242
TspRI CASTG 3 cut(s) 228, 362, 1396
VneI GTGCAC 1 cut(s) 399
VspI ATTAAT 1 cut(s) 1091
XagI CCTNNNNNAGG 1 cut(s) 710
XapI RAATTY 4 cut(s) 323, 541, 563, 609
XceI RCATGY 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 2 cut(s) 129, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.