Rh7AG401900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006189
Physical Location & Seq
Forward (+)
52955884 .. 52959884
4001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG401900.1

Sequence Viewer

Length: 1506 bp
ATGCTTGATCTGTTTCTGTCGGAGCCCAATTGGAAAGAAGATGACTCTGCCGAGATGAGAGTGTCTCTCTTGAACAAATTGGAATCTGTTATTTGGTCGTTGATGGCTTCTGGAGGTCGATCAGAGGCTAGACTATGGCTTTGCAACACTATAGCTAGCATAAGTTCTATCACTCATCATCATCAACGTGAGTTATTTATCGACTTATTGAGATCTAAGCCGCTGAAGAAGCGCTTAGCAACTCAGCTCCTGCACATGATATTTGATAATAGGCCACAAAAGGCAGGGTCTGTCATATCCAAGAGAAGCTATGCACTTAGGAAATTTTTTCAAGGAAACCCAACACGAATATCTCAGTGGTTTTCCAAAACTGGTTCTGGATTGAGGCAGGGACAAGGTGCTAATGCGTTATCCCAATTTGCTTTTGTTAATCGAGATAATTGTTGGGAGGAGCTTGAGTGGAAGGGAAAACATGGGCAATCACCGGCGGTAGTTGCTACAAAACCCCATTACTTTCTTGATTTGGACGTCCAGCAAACCGTAGAGAATTTCCTTGAAAATGTGCCTGAATTTTGGTCATCCAATGAGTTTTCTGAGTCACTTAAAGGTGGAGAAATTTTGATCATTGATAGAAACTTTTTTGTTCTATATTTTCTGGATTTGATGTACAAAGAAGATGCCAGAGATGTATGGGAAGTTACGAATGAGTTCCTGGAGGAGGAGCACTTTTCTTCCCTGTGTAAACATCTTCTTATTACTCTTGAGGAGTCAGACTTATGTGATTTTCTTAAAATGCTTCATAAACATGTCAAGCCTAAAGTAGAATGCAAAGATCCTATGGACTCCTCTTATTTGTTTGAAGTTGTAGTATCCAGGTGCAGTGATTGTGGGTGCTTTGATCAGATATTACTGTTAAATGCAGTTTTTAACTATGGCCGCCAGCTTCTACGACTCTTACGAGATGAAGGAGCGCCTGAGGATCAAGCAAAACTACAAGATATTGTGTCCGAGATTTGTGCAATCCCAGGCAATGAAAATAGCATGGCACCAATAATTAAAGGGGGCTCTAAACTGAAGACTATAGAAGCATTTAAATTGATGGGGCTTCAGTCATGGGTCCTCTTTTTTAGTTTATCAGAAAAATGCCAGACTCCTCAGTCTTGGGAATCATTATTTGTTGATAATGAAATAAGTTTTCGAAAGTATGATAAATATTCATTTCTTCATGATAATGAAGTTGCCGGAGAAGGTGGTTCTGATTTGGATTATAGAGGGTCCAAAAAAGTTAAGCGAAGGAAAAAGACTAGTAGTAGAAGGAAAAGAAGAAGGGACTTTGATCATGATGACAATGAGTTGCTGGATTCCGATACTACAAATAATAGGCTGGATTTGCATTCTAGTGTGGGAAGTTGGTTGCTCTCACTTGATGGGTATTCTGCATCATGGAACAGTGTAAGTATCTCACATTTTTTTTTCTTTGGTCTGAACTTTATTGGAGACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

501

Amino Acids

57.7

Weight (kDa)

5.92

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1156
AatII GACGTC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 1045
AciI CCGC 3 cut(s) 221, 488, 937
AclWI GGATC 2 cut(s) 827, 987
AcoI YGGCCR 1 cut(s) 934
AcsI RAATTY 4 cut(s) 323, 547, 569, 615
AcuI CTGAAG 3 cut(s) 245, 1091, 1094
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 1 cut(s) 668
AfeI AGCGCT 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 718
AflIII ACRYGT 1 cut(s) 805
AgsI TTSAA 4 cut(s) 73, 332, 557, 860
AhlI ACTAGT 1 cut(s) 1304
AjnI CCWGG 3 cut(s) 711, 872, 1024
AluBI AGCT 5 cut(s) 155, 247, 309, 454, 943
AluI AGCT 5 cut(s) 155, 247, 309, 454, 943
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 2 cut(s) 69, 1491
AlwI GGATC 2 cut(s) 827, 987
AlwNI CAGNNNCTG 2 cut(s) 250, 290
Aor51HI AGCGCT 1 cut(s) 233
AoxI GGCC 2 cut(s) 272, 934
ApoI RAATTY 4 cut(s) 323, 547, 569, 615
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
Asp700I GAANNNNTTC 1 cut(s) 707
AspLEI GCGC 2 cut(s) 234, 973
AspS9I GGNCC 2 cut(s) 1117, 1275
AsuHPI GGTGA 1 cut(s) 474
AsuII TTCGAA 1 cut(s) 1198
AsuNHI GCTAGC 1 cut(s) 155
AvaII GGWCC 2 cut(s) 1117, 1275
AxyI CCTNAGG 1 cut(s) 975
BanI GGYRCC 1 cut(s) 1045
BanII GRGCYC 2 cut(s) 27, 1067
BbsI GAAGAC 1 cut(s) 1082
Bbv12I GWGCWC 1 cut(s) 726
BccI CCATC 3 cut(s) 97, 1093, 1421
BcgI CGANNNNNNTGC 2 cut(s) 998, 1032
BciT130I CCWGG 3 cut(s) 713, 874, 1026
BciVI GTATCC 1 cut(s) 880
BclI TGATCA 3 cut(s) 621, 898, 1336
BcoDI GTCTC 2 cut(s) 69, 1491
BcuI ACTAGT 1 cut(s) 1304
BfaI CTAG 4 cut(s) 129, 156, 1305, 1398
BfmI CTRYAG 2 cut(s) 150, 1080
BfoI RGCGCY 2 cut(s) 235, 974
BfuI GTATCC 1 cut(s) 880
BglII AGATCT 1 cut(s) 212
BisI GCNGC 2 cut(s) 221, 937
BlpI GCTNAGC 1 cut(s) 235
BlsI GCNGC 2 cut(s) 222, 938
Bme1390I CCNGG 3 cut(s) 713, 874, 1026
Bme18I GGWCC 2 cut(s) 1117, 1275
BmgT120I GGNCC 2 cut(s) 1117, 1275
BmiI GGNNCC 4 cut(s) 24, 1047, 1118, 1276
BmrFI CCNGG 3 cut(s) 713, 874, 1026
BmsI GCATC 2 cut(s) 667, 1448
BmtI GCTAGC 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 1082
BplI GAGNNNNNCTC 4 cut(s) 49, 81, 51, 83
BpmI CTGGAG 2 cut(s) 132, 734
Bpu1102I GCTNAGC 1 cut(s) 235
Bpu14I TTCGAA 1 cut(s) 1198
BpuEI CTTGAG 2 cut(s) 476, 782
BsaHI GRCGYC 1 cut(s) 528
BsaJI CCNNGG 1 cut(s) 1024
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 1 cut(s) 718
Bse118I RCCGGY 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 376
Bse21I CCTNAGG 1 cut(s) 975
Bse3DI GCAATG 1 cut(s) 1036
BseBI CCWGG 3 cut(s) 713, 874, 1026
BseDI CCNNGG 1 cut(s) 1024
BseGI GGATG 1 cut(s) 578
BseLI CCNNNNNNNGG 1 cut(s) 718
BseMI GCAATG 1 cut(s) 1036
BseMII CTCAG 5 cut(s) 257, 368, 585, 966, 1169
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 6 cut(s) 464, 731, 734, 779, 835, 1143
BsgI GTGCAG 2 cut(s) 236, 898
BshFI GGCC 2 cut(s) 274, 936
BshNI GGYRCC 1 cut(s) 1045
BsiHKAI GWGCWC 1 cut(s) 726
BsiSI CCGG 2 cut(s) 485, 1242
BslFI GGGAC 2 cut(s) 405, 1343
BslI CCNNNNNNNGG 1 cut(s) 718
BsmAI GTCTC 2 cut(s) 69, 1491
BsmFI GGGAC 2 cut(s) 405, 1343
BsmI GAATGC 2 cut(s) 830, 1393
BsnI GGCC 2 cut(s) 274, 936
Bsp119I TTCGAA 1 cut(s) 1198
Bsp1286I GDGCHC 3 cut(s) 27, 726, 1067
Bsp1407I TGTACA 1 cut(s) 666
Bsp143I GATC 8 cut(s) 7, 119, 212, 621, 832, 898, 979, 1336
Bsp1720I GCTNAGC 1 cut(s) 235
BspACI CCGC 3 cut(s) 221, 488, 937
BspANI GGCC 2 cut(s) 274, 936
BspCNI CTCAG 5 cut(s) 256, 367, 586, 967, 1168
BspHI TCATGA 2 cut(s) 1225, 1339
BspLI GGNNCC 4 cut(s) 24, 1047, 1118, 1276
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 2 cut(s) 827, 987
BspT104I TTCGAA 1 cut(s) 1198
BspT107I GGYRCC 1 cut(s) 1045
BsrDI GCAATG 1 cut(s) 1036
BsrFI RCCGGY 1 cut(s) 484
BsrGI TGTACA 1 cut(s) 666
BsrI ACTGG 1 cut(s) 376
BssAI RCCGGY 1 cut(s) 484
BssECI CCNNGG 1 cut(s) 1024
BssMI GATC 8 cut(s) 7, 119, 212, 621, 832, 898, 979, 1336
BssNI GRCGYC 1 cut(s) 528
Bst2UI CCWGG 3 cut(s) 713, 874, 1026
Bst4CI ACNGT 3 cut(s) 541, 912, 1451
BstACI GRCGYC 1 cut(s) 528
BstAUI TGTACA 1 cut(s) 666
BstBI TTCGAA 1 cut(s) 1198
BstC8I GCNNGC 2 cut(s) 157, 941
BstDEI CTNAG 8 cut(s) 216, 235, 243, 317, 354, 594, 975, 1155
BstENI CCTNNNNNAGG 1 cut(s) 716
BstF5I GGATG 1 cut(s) 578
BstH2I RGCGCY 2 cut(s) 235, 974
BstHHI GCGC 2 cut(s) 234, 973
BstKTI GATC 8 cut(s) 10, 122, 215, 624, 835, 901, 982, 1339
BstMAI GTCTC 2 cut(s) 69, 1491
BstMBI GATC 8 cut(s) 7, 119, 212, 621, 832, 898, 979, 1336
BstMWI GCNNNNNNNGC 3 cut(s) 229, 494, 1390
BstNI CCWGG 3 cut(s) 713, 874, 1026
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 3 cut(s) 711, 872, 1024
BstSFI CTRYAG 2 cut(s) 150, 1080
BstV2I GAAGAC 1 cut(s) 1082
BstX2I RGATCY 2 cut(s) 212, 832
BstYI RGATCY 2 cut(s) 212, 832
Bsu36I CCTNAGG 1 cut(s) 975
BsuI GTATCC 1 cut(s) 880
BsuRI GGCC 2 cut(s) 274, 936
BtsCI GGATG 1 cut(s) 578
BtsI GCAGTG 1 cut(s) 886
BtsIMutI CAGTG 3 cut(s) 362, 886, 1456
Cac8I GCNNGC 2 cut(s) 157, 941
CaiI CAGNNNCTG 2 cut(s) 250, 290
CciI TCATGA 2 cut(s) 1225, 1339
CfoI GCGC 2 cut(s) 234, 973
Cfr10I RCCGGY 1 cut(s) 484
Cfr13I GGNCC 2 cut(s) 1117, 1275
Csp6I GTAC 1 cut(s) 667
CviAII CATG 8 cut(s) 256, 473, 806, 1042, 1113, 1226, 1340, 1443
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 8 cut(s) 216, 235, 243, 317, 354, 594, 975, 1155
DpnI GATC 8 cut(s) 9, 121, 214, 623, 834, 900, 981, 1338
DpnII GATC 8 cut(s) 7, 119, 212, 621, 832, 898, 979, 1336
DraI TTTAAA 1 cut(s) 1093
DrdI GACNNNNNNGTC 1 cut(s) 1156
DseDI GACNNNNNNGTC 1 cut(s) 1156
EaeI YGGCCR 1 cut(s) 934
Eco24I GRGCYC 2 cut(s) 27, 1067
Eco47I GGWCC 2 cut(s) 1117, 1275
Eco47III AGCGCT 1 cut(s) 233
Eco57I CTGAAG 3 cut(s) 245, 1091, 1094
Eco81I CCTNAGG 1 cut(s) 975
EcoNI CCTNNNNNAGG 1 cut(s) 716
EcoO109I RGGNCCY 1 cut(s) 1117
EcoRII CCWGG 3 cut(s) 711, 872, 1024
EcoT38I GRGCYC 2 cut(s) 27, 1067
FaeI CATG 8 cut(s) 259, 476, 809, 1045, 1116, 1229, 1343, 1446
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 2 cut(s) 405, 1343
FatI CATG 8 cut(s) 255, 472, 805, 1041, 1112, 1225, 1339, 1442
FbaI TGATCA 3 cut(s) 621, 898, 1336
Fnu4HI GCNGC 2 cut(s) 221, 937
FokI GGATG 1 cut(s) 565
FriOI GRGCYC 2 cut(s) 27, 1067
Fsp4HI GCNGC 2 cut(s) 221, 937
FspBI CTAG 4 cut(s) 129, 156, 1305, 1398
GlaI GCGC 2 cut(s) 233, 972
GluI GCNGC 2 cut(s) 221, 937
GsuI CTGGAG 2 cut(s) 132, 734
HaeII RGCGCY 2 cut(s) 235, 974
HaeIII GGCC 2 cut(s) 274, 936
HapII CCGG 2 cut(s) 485, 1242
HhaI GCGC 2 cut(s) 234, 973
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 8 cut(s) 259, 476, 809, 1045, 1116, 1229, 1343, 1446
Hin6I GCGC 2 cut(s) 232, 971
HinP1I GCGC 2 cut(s) 232, 971
HpaII CCGG 2 cut(s) 485, 1242
HphI GGTGA 1 cut(s) 474
Hpy166II GTNNAC 1 cut(s) 743
Hpy188III TCNNGA 9 cut(s) 70, 111, 378, 434, 518, 656, 761, 1226, 1340
Hpy8I GTNNAC 1 cut(s) 743
HpyAV CCTTC 6 cut(s) 457, 959, 1241, 1287, 1308, 1320
HpyCH4III ACNGT 3 cut(s) 541, 912, 1451
HpyCH4IV ACGT 2 cut(s) 187, 528
HpyCH4V TGCA 9 cut(s) 144, 253, 314, 828, 879, 920, 1019, 1393, 1439
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 494, 1390
HpyF3I CTNAG 8 cut(s) 216, 235, 243, 317, 354, 594, 975, 1155
HpySE526I ACGT 2 cut(s) 187, 528
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 8 cut(s) 259, 476, 809, 1045, 1116, 1229, 1343, 1446
HspAI GCGC 2 cut(s) 232, 971
Ksp22I TGATCA 3 cut(s) 621, 898, 1336
Kzo9I GATC 8 cut(s) 7, 119, 212, 621, 832, 898, 979, 1336
LmnI GCTCC 5 cut(s) 22, 252, 451, 721, 968
LweI GCATC 2 cut(s) 667, 1448
MaeI CTAG 4 cut(s) 129, 156, 1305, 1398
MaeII ACGT 2 cut(s) 187, 528
MaeIII GTNAC 2 cut(s) 597, 697
MalI GATC 8 cut(s) 9, 121, 214, 623, 834, 900, 981, 1338
MboI GATC 8 cut(s) 7, 119, 212, 621, 832, 898, 979, 1336
MboII GAAGA 8 cut(s) 50, 238, 686, 723, 740, 1087, 1214, 1335
MfeI CAATTG 1 cut(s) 28
MflI RGATCY 2 cut(s) 212, 832
MhlI GDGCHC 3 cut(s) 27, 726, 1067
MlyI GAGTC 7 cut(s) 38, 605, 776, 836, 945, 1144, 1493
MroXI GAANNNNTTC 1 cut(s) 707
MseI TTAA 8 cut(s) 429, 603, 789, 914, 927, 1056, 1092, 1287
MslI CAYNNNNRTG 4 cut(s) 186, 804, 1230, 1398
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 2 cut(s) 485, 1242
MspR9I CCNGG 3 cut(s) 713, 874, 1026
MunI CAATTG 1 cut(s) 28
Mva1269I GAATGC 2 cut(s) 830, 1393
MvaI CCWGG 3 cut(s) 713, 874, 1026
MwoI GCNNNNNNNGC 3 cut(s) 229, 494, 1390
NdeII GATC 8 cut(s) 7, 119, 212, 621, 832, 898, 979, 1336
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 8 cut(s) 259, 476, 809, 1045, 1116, 1229, 1343, 1446
NlaIV GGNNCC 4 cut(s) 24, 1047, 1118, 1276
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 597
NspI RCATGY 1 cut(s) 809
NspV TTCGAA 1 cut(s) 1198
PagI TCATGA 2 cut(s) 1225, 1339
PciI ACATGT 1 cut(s) 805
PcsI WCGNNNNNNNCGW 1 cut(s) 955
PctI GAATGC 2 cut(s) 830, 1393
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 3 cut(s) 83, 1166, 1361
PfoI TCCNGGA 1 cut(s) 711
PkrI GCNGC 2 cut(s) 222, 938
PleI GAGTC 7 cut(s) 38, 604, 775, 836, 945, 1144, 1493
PpsI GAGTC 7 cut(s) 38, 604, 775, 836, 945, 1144, 1493
PpuMI RGGWCCY 1 cut(s) 1117
PscI ACATGT 1 cut(s) 805
Psp5II RGGWCCY 1 cut(s) 1117
Psp6I CCWGG 3 cut(s) 711, 872, 1024
PspGI CCWGG 3 cut(s) 711, 872, 1024
PspN4I GGNNCC 4 cut(s) 24, 1047, 1118, 1276
PspPI GGNCC 2 cut(s) 1117, 1275
PspPPI RGGWCCY 1 cut(s) 1117
PstNI CAGNNNCTG 2 cut(s) 250, 290
PsuI RGATCY 2 cut(s) 212, 832
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
RseI CAYNNNNRTG 4 cut(s) 186, 804, 1230, 1398
SaqAI TTAA 8 cut(s) 429, 603, 789, 914, 927, 1056, 1092, 1287
SatI GCNGC 2 cut(s) 221, 937
Sau3AI GATC 8 cut(s) 7, 119, 212, 621, 832, 898, 979, 1336
Sau96I GGNCC 2 cut(s) 1117, 1275
SchI GAGTC 7 cut(s) 38, 605, 776, 836, 945, 1144, 1493
ScrFI CCNGG 3 cut(s) 713, 874, 1026
SduI GDGCHC 3 cut(s) 27, 726, 1067
SfaNI GCATC 2 cut(s) 667, 1448
SfcI CTRYAG 2 cut(s) 150, 1080
SfuI TTCGAA 1 cut(s) 1198
SgrAI CRCCGGYG 1 cut(s) 484
SinI GGWCC 2 cut(s) 1117, 1275
SmiI ATTTAAAT 1 cut(s) 1093
SmiMI CAYNNNNRTG 4 cut(s) 186, 804, 1230, 1398
SmlI CTYRAG 2 cut(s) 455, 761
SmoI CTYRAG 2 cut(s) 455, 761
SpeI ACTAGT 1 cut(s) 1304
SsiI CCGC 3 cut(s) 221, 488, 937
SspI AATATT 1 cut(s) 1214
SspMI CTAG 4 cut(s) 129, 156, 1305, 1398
StyD4I CCNGG 3 cut(s) 711, 872, 1024
SwaI ATTTAAAT 1 cut(s) 1093
TaaI ACNGT 3 cut(s) 541, 912, 1451
TaiI ACGT 2 cut(s) 190, 531
TaqI TCGA 4 cut(s) 118, 201, 433, 1198
TatI WGTACW 1 cut(s) 666
TauI GCSGC 2 cut(s) 223, 939
TfiI GAWTC 3 cut(s) 83, 1166, 1361
Tru1I TTAA 8 cut(s) 429, 603, 789, 914, 927, 1056, 1092, 1287
Tru9I TTAA 8 cut(s) 429, 603, 789, 914, 927, 1056, 1092, 1287
TscAI CASTG 3 cut(s) 362, 886, 1456
TseFI GTSAC 1 cut(s) 597
Tsp45I GTSAC 1 cut(s) 597
TspDTI ATGAA 7 cut(s) 788, 978, 1047, 1200, 1206, 1214, 1248
TspRI CASTG 3 cut(s) 362, 886, 1456
VpaK11BI GGWCC 2 cut(s) 1117, 1275
XagI CCTNNNNNAGG 1 cut(s) 716
XapI RAATTY 4 cut(s) 323, 547, 569, 615
XceI RCATGY 1 cut(s) 809
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 4 cut(s) 129, 156, 1305, 1398
ZraI GACGTC 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.