FvH4_5g35361

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018449
Physical Location & Seq
Reverse (-)
25901220 .. 25901978
759 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35361.t1

Sequence Viewer

Length: 555 bp
ATGGTGGTCGATTCCCGGGAGGAGAAGCGGCTCAGCGCCACCGTGGAACTAGTGGAACCCATCAACACACCAAATCTGCAAGAAGCATTTTCACCGGCTCGACTTCAAGTCCAGTTTCTCTACACTACCCAACACCAAATCTTCGCCAGTACATCGTCTACCAAGAGCACATACTTCATCGAATCATCACCACAAGAAACCGGCTGGCCAACTCTAATCCATCATTATGAGGACGCCGGGAAGAAACTGTGCGGTGAGTTCGATCTGAACGTGCTACTGAGTACGGTTGATGAACTGGGTGCAAAGCTTTCTCCATTTCTTTCGCAATTTAGAGTCGAGCAAGGTAGCGAGGAGCACGCCCTTATAGTTGGTGAGGTTAAGCGCGTGGGGATGGGAATTGGTCCGGAGAGGAAGAATCAGGGTTTGTTTGCGTTGCAAACATGGCTGGATCGGAAAAAACGCTCTTGTCCTCTTTGTCGATCTGATAAGATTCCTACTGGTCTTGTAAGACTATCCCTCACAGATATGTACCATCGTGGTATTATCATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.71

Weight (kDa)

6.52

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g35350 FvH4_5g35361
rosa_chinensis RchiOBHm_Chr7g0236701
rosa_laevigata RLG00000001067
rosa_multiflora Rmu_sc0002536.1_g000017
rosa_roxburghii Rroxscaffold_3G00224950
rosa_rugosa Rorug07G0298300
rosa_samantha Rh7AG453700 Rh7CG472200 Rh7DG441100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 158
AccII CGCG 1 cut(s) 384
AccIII TCCGGA 1 cut(s) 403
AciI CCGC 2 cut(s) 28, 252
AclWI GGATC 1 cut(s) 456
AcoI YGGCCR 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 3 cut(s) 151, 283, 530
AgsI TTSAA 1 cut(s) 107
AhdI GACNNNNNGTC 1 cut(s) 107
AhlI ACTAGT 1 cut(s) 49
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw21I GWGCWC 2 cut(s) 170, 357
AlwI GGATC 1 cut(s) 456
Ama87I CYCGRG 1 cut(s) 15
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 1 cut(s) 206
AspLEI GCGC 2 cut(s) 38, 384
AspS9I GGNCC 1 cut(s) 401
AsuC2I CCSGG 3 cut(s) 16, 17, 238
AsuHPI GGTGA 4 cut(s) 84, 180, 266, 383
AvaI CYCGRG 1 cut(s) 15
AvaII GGWCC 1 cut(s) 401
BalI TGGCCA 1 cut(s) 208
Bbv12I GWGCWC 2 cut(s) 170, 357
BccI CCATC 4 cut(s) 68, 228, 385, 540
BcnI CCSGG 3 cut(s) 16, 17, 238
BcuI ACTAGT 1 cut(s) 49
BfaI CTAG 1 cut(s) 50
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 1 cut(s) 29
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 1 cut(s) 30
Bme1390I CCNGG 3 cut(s) 16, 17, 238
Bme18I GGWCC 1 cut(s) 401
BmeRI GACNNNNNGTC 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 57
BmrFI CCNGG 3 cut(s) 16, 17, 238
BmrI ACTGGG 1 cut(s) 305
BmuI ACTGGG 1 cut(s) 305
Bpu1102I GCTNAGC 1 cut(s) 32
BpuMI CCSGG 3 cut(s) 16, 17, 238
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 15, 42
BsaWI WCCGGW 1 cut(s) 403
Bse118I RCCGGY 2 cut(s) 94, 200
Bse1I ACTGG 4 cut(s) 112, 147, 300, 502
BseAI TCCGGA 1 cut(s) 403
BseDI CCNNGG 2 cut(s) 15, 42
BseGI GGATG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 46, 269
BseNI ACTGG 4 cut(s) 112, 147, 300, 502
BseRI GAGGAG 2 cut(s) 35, 365
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 170, 357
BsiHKCI CYCGRG 1 cut(s) 15
BsiSI CCGG 5 cut(s) 16, 95, 201, 237, 404
BsnI GGCC 1 cut(s) 208
BsoBI CYCGRG 1 cut(s) 15
Bsp1286I GDGCHC 2 cut(s) 170, 357
Bsp13I TCCGGA 1 cut(s) 403
Bsp143I GATC 3 cut(s) 262, 448, 479
Bsp1720I GCTNAGC 1 cut(s) 32
BspACI CCGC 2 cut(s) 28, 252
BspANI GGCC 1 cut(s) 208
BspCNI CTCAG 2 cut(s) 45, 270
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 57
BspPI GGATC 1 cut(s) 456
BsrFI RCCGGY 2 cut(s) 94, 200
BsrI ACTGG 4 cut(s) 112, 147, 300, 502
BssAI RCCGGY 2 cut(s) 94, 200
BssECI CCNNGG 2 cut(s) 15, 42
BssMI GATC 3 cut(s) 262, 448, 479
BssNI GRCGYC 1 cut(s) 234
Bst4CI ACNGT 3 cut(s) 43, 249, 286
BstACI GRCGYC 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 206, 357
BstDEI CTNAG 2 cut(s) 32, 278
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 1 cut(s) 396
BstFNI CGCG 1 cut(s) 384
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 384
BstKTI GATC 3 cut(s) 265, 451, 482
BstMBI GATC 3 cut(s) 262, 448, 479
BstMWI GCNNNNNNNGC 1 cut(s) 442
BstSCI CCNGG 3 cut(s) 14, 15, 236
BstUI CGCG 1 cut(s) 384
BsuRI GGCC 1 cut(s) 208
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 206, 357
CfoI GCGC 2 cut(s) 38, 384
Cfr10I RCCGGY 2 cut(s) 94, 200
Cfr13I GGNCC 1 cut(s) 401
Cfr9I CCCGGG 1 cut(s) 15
CseI GACGC 1 cut(s) 242
Csp6I GTAC 3 cut(s) 150, 282, 529
CviAII CATG 1 cut(s) 441
CviJI RGCY 6 cut(s) 31, 98, 204, 208, 307, 445
CviKI_1 RGCY 6 cut(s) 31, 98, 204, 208, 307, 445
CviQI GTAC 3 cut(s) 150, 282, 529
DdeI CTNAG 2 cut(s) 32, 278
DpnI GATC 3 cut(s) 264, 450, 481
DpnII GATC 3 cut(s) 262, 448, 479
DriI GACNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 206
Eam1105I GACNNNNNGTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 15
FaeI CATG 1 cut(s) 444
FaiI YATR 5 cut(s) 172, 228, 365, 442, 527
FatI CATG 1 cut(s) 440
FblI GTMKAC 1 cut(s) 158
Fnu4HI GCNGC 1 cut(s) 29
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 1 cut(s) 29
FspBI CTAG 1 cut(s) 50
GlaI GCGC 2 cut(s) 37, 383
GluI GCNGC 1 cut(s) 29
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 1 cut(s) 208
HapII CCGG 5 cut(s) 16, 95, 201, 237, 404
HgaI GACGC 1 cut(s) 242
HhaI GCGC 2 cut(s) 38, 384
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 1 cut(s) 444
Hin6I GCGC 2 cut(s) 36, 382
HinP1I GCGC 2 cut(s) 36, 382
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 5 cut(s) 11, 182, 333, 415, 490
HpaII CCGG 5 cut(s) 16, 95, 201, 237, 404
HphI GGTGA 4 cut(s) 84, 180, 266, 383
Hpy166II GTNNAC 1 cut(s) 159
Hpy188I TCNGA 3 cut(s) 267, 453, 484
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 1 cut(s) 159
HpyCH4III ACNGT 3 cut(s) 43, 249, 286
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 79, 302, 436
HpyF10VI GCNNNNNNNGC 1 cut(s) 442
HpyF3I CTNAG 2 cut(s) 32, 278
HpySE526I ACGT 1 cut(s) 270
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 1 cut(s) 444
HspAI GCGC 2 cut(s) 36, 382
Kpn2I TCCGGA 1 cut(s) 403
Kzo9I GATC 3 cut(s) 262, 448, 479
LmnI GCTCC 1 cut(s) 352
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 270
MalI GATC 3 cut(s) 264, 450, 481
MboI GATC 3 cut(s) 262, 448, 479
MboII GAAGA 3 cut(s) 133, 253, 424
MhlI GDGCHC 2 cut(s) 170, 357
MlsI TGGCCA 1 cut(s) 208
MluCI AATT 2 cut(s) 326, 396
MluNI TGGCCA 1 cut(s) 208
MlyI GAGTC 1 cut(s) 342
MnlI CCTC 7 cut(s) 13, 223, 343, 367, 402, 480, 527
Mox20I TGGCCA 1 cut(s) 208
MroI TCCGGA 1 cut(s) 403
MscI TGGCCA 1 cut(s) 208
MseI TTAA 2 cut(s) 378, 553
MslI CAYNNNNRTG 2 cut(s) 225, 524
Msp20I TGGCCA 1 cut(s) 208
MspI CCGG 5 cut(s) 16, 95, 201, 237, 404
MspR9I CCNGG 3 cut(s) 16, 17, 238
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 442
NciI CCSGG 3 cut(s) 16, 17, 238
NdeII GATC 3 cut(s) 262, 448, 479
NlaIII CATG 1 cut(s) 444
NlaIV GGNNCC 1 cut(s) 57
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PfeI GAWTC 4 cut(s) 11, 182, 415, 490
PkrI GCNGC 1 cut(s) 30
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 57
PspPI GGNCC 1 cut(s) 401
RsaI GTAC 3 cut(s) 151, 283, 530
RsaNI GTAC 3 cut(s) 150, 282, 529
RseI CAYNNNNRTG 2 cut(s) 225, 524
SaqAI TTAA 2 cut(s) 378, 553
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 3 cut(s) 262, 448, 479
Sau96I GGNCC 1 cut(s) 401
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 3 cut(s) 16, 17, 238
SduI GDGCHC 2 cut(s) 170, 357
SetI ASST 4 cut(s) 273, 309, 346, 378
SinI GGWCC 1 cut(s) 401
SmaI CCCGGG 1 cut(s) 17
SmiMI CAYNNNNRTG 2 cut(s) 225, 524
SpeI ACTAGT 1 cut(s) 49
Sse9I AATT 2 cut(s) 326, 396
SsiI CCGC 2 cut(s) 28, 252
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 3 cut(s) 14, 15, 236
TaaI ACNGT 3 cut(s) 43, 249, 286
TaiI ACGT 1 cut(s) 273
TaqI TCGA 6 cut(s) 9, 100, 180, 261, 336, 478
TasI AATT 2 cut(s) 326, 396
TatI WGTACW 1 cut(s) 149
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 4 cut(s) 11, 182, 415, 490
Tru1I TTAA 2 cut(s) 378, 553
Tru9I TTAA 2 cut(s) 378, 553
TspDTI ATGAA 2 cut(s) 166, 306
TspMI CCCGGG 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 401
XmaI CCCGGG 1 cut(s) 15
XmiI GTMKAC 1 cut(s) 158
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.