Rroxscaffold_3G00224950

finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0018449
Physical Location & Seq
Forward (+)
8214918 .. 8215759
842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00224950.1

Sequence Viewer

Length: 561 bp
ATGGATTCGCAGGATGTGGTGGTCACTTCCCGGCGGGAGGAGCGGTTCAGCGCCTCCGTGGAACAAGTCGAACCCATCTACCCACCAGAAGACTGCCCAACCCTGATCCACCATTATGAGGACCCCGTGATGAAACTGTGTGGTGAATTCGATCTGCAAGTTCTACCGTTTCTTTCGCAATTTGAAATCACGCAAGGCAGCGAGGAGCACACCCGAATATTGGGTGAGGTTAAGCGTGTGGGCAGGGAATTTGGCCCGGAGAGGAAGAATCAAGGGTTATTTGGCTTGCAAGTTTCGATTTTAAGAGTATACACGACATATCGTTGCGAGAAGATGAGGGTTAGAGTTCCAGGGGAAGAGATCTGTAGTGTGTGCTTGGAAGAGTTTAGGAGTTGCCCGCCTTGCACTGCCTTGCCTTGCTCACATGTGTTTCATAGCGAATGCATTCGGACATGGCTGGGACGGAATGAACGCTCTTGTCCTCTTTGCAGATTTCAGTTGCCTACTATAGTTGAACCATCCTTCACATATATGTTCCATCTTGGGCTTAATCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

186

Amino Acids

21.49

Weight (kDa)

5.56

Isoelectric Point (pI)

69.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 116 - 164 2.4e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 122 - 164 6.3e-12 Ring finger domain
zf-RING_11 PF17123 122 - 149 4.4e-08 RING-like zinc finger
zf-C3HC4 PF00097 122 - 163 7.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 122 - 163 8.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 122 - 161 2.1e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g35350 FvH4_5g35361
rosa_chinensis RchiOBHm_Chr7g0236701
rosa_laevigata RLG00000001067
rosa_multiflora Rmu_sc0002536.1_g000017
rosa_roxburghii Rroxscaffold_3G00224950
rosa_rugosa Rorug07G0298300
rosa_samantha Rh7AG453700 Rh7CG472200 Rh7DG441100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 309
AciI CCGC 3 cut(s) 34, 43, 398
AclWI GGATC 1 cut(s) 100
AcsI RAATTY 2 cut(s) 146, 248
AfiI CCNNNNNNNGG 3 cut(s) 37, 118, 220
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 2 cut(s) 185, 515
AjnI CCWGG 1 cut(s) 349
AloI GAACNNNNNNTCC 2 cut(s) 29, 61
Alw21I GWGCWC 1 cut(s) 210
AlwI GGATC 1 cut(s) 100
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 198
ApoI RAATTY 2 cut(s) 146, 248
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 2 cut(s) 121, 254
AsuC2I CCSGG 2 cut(s) 31, 257
AsuHPI GGTGA 2 cut(s) 155, 236
AvaII GGWCC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 1 cut(s) 210
BccI CCATC 3 cut(s) 83, 526, 546
BciT130I CCWGG 1 cut(s) 351
BcnI CCSGG 2 cut(s) 31, 257
BfmI CTRYAG 2 cut(s) 364, 507
BfoI RGCGCY 1 cut(s) 54
BglII AGATCT 1 cut(s) 360
BisI GCNGC 1 cut(s) 199
BlsI GCNGC 1 cut(s) 200
Bme1390I CCNGG 3 cut(s) 31, 257, 351
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 2 cut(s) 121, 254
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 3 cut(s) 31, 257, 351
BpiI GAAGAC 1 cut(s) 96
BpuMI CCSGG 2 cut(s) 31, 257
BsaJI CCNNGG 2 cut(s) 57, 350
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 118, 220
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 2 cut(s) 57, 350
BseGI GGATG 2 cut(s) 19, 518
BseLI CCNNNNNNNGG 3 cut(s) 37, 118, 220
BseRI GAGGAG 2 cut(s) 53, 218
BseXI GCAGC 1 cut(s) 210
BseYI CCCAGC 1 cut(s) 457
BshFI GGCC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 2 cut(s) 31, 257
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 3 cut(s) 37, 118, 220
BsmFI GGGAC 1 cut(s) 474
BsmI GAATGC 2 cut(s) 444, 446
BsnI GGCC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 3 cut(s) 105, 151, 360
BspACI CCGC 3 cut(s) 34, 43, 398
BspANI GGCC 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 123
BspPI GGATC 1 cut(s) 100
BsrBI CCGCTC 1 cut(s) 43
BssECI CCNNGG 2 cut(s) 57, 350
BssMI GATC 3 cut(s) 105, 151, 360
BssNAI GTATAC 1 cut(s) 310
Bst1107I GTATAC 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 138, 168
Bst6I CTCTTC 2 cut(s) 351, 375
BstC8I GCNNGC 2 cut(s) 287, 398
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 19, 518
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 3 cut(s) 108, 154, 363
BstMBI GATC 3 cut(s) 105, 151, 360
BstMWI GCNNNNNNNGC 2 cut(s) 40, 402
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 29, 255, 349
BstSFI CTRYAG 2 cut(s) 364, 507
BstV1I GCAGC 1 cut(s) 210
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
BstZ17I GTATAC 1 cut(s) 310
BsuRI GGCC 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 19, 518
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 2 cut(s) 287, 398
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 121, 254
CviAII CATG 2 cut(s) 425, 453
CviJI RGCY 4 cut(s) 255, 285, 457, 547
CviKI_1 RGCY 4 cut(s) 255, 285, 457, 547
DpnI GATC 3 cut(s) 107, 153, 362
DpnII GATC 3 cut(s) 105, 151, 360
Eam1104I CTCTTC 2 cut(s) 351, 375
EarI CTCTTC 2 cut(s) 351, 375
Eco47I GGWCC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 446
FaeI CATG 2 cut(s) 428, 456
FaqI GGGAC 1 cut(s) 474
FatI CATG 2 cut(s) 424, 452
FauI CCCGC 2 cut(s) 27, 405
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 1 cut(s) 199
FokI GGATG 2 cut(s) 26, 505
Fsp4HI GCNGC 1 cut(s) 199
GlaI GCGC 1 cut(s) 52
GluI GCNGC 1 cut(s) 199
GsaI CCCAGC 1 cut(s) 461
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 255
HapII CCGG 2 cut(s) 31, 257
HhaI GCGC 1 cut(s) 53
Hin1II CATG 2 cut(s) 428, 456
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HinfI GANTC 2 cut(s) 5, 268
HpaII CCGG 2 cut(s) 31, 257
HphI GGTGA 2 cut(s) 155, 236
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 1 cut(s) 450
Hpy8I GTNNAC 1 cut(s) 310
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 2 cut(s) 138, 168
HpyCH4V TGCA 5 cut(s) 157, 289, 405, 444, 489
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 402
Hsp92II CATG 2 cut(s) 428, 456
HspAI GCGC 1 cut(s) 51
Kzo9I GATC 3 cut(s) 105, 151, 360
LmnI GCTCC 2 cut(s) 40, 205
LpnPI CCDG 8 cut(s) 44, 99, 116, 229, 270, 336, 363, 443
Lsp1109I GCAGC 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 22
MalI GATC 3 cut(s) 107, 153, 362
MbiI CCGCTC 1 cut(s) 43
MboI GATC 3 cut(s) 105, 151, 360
MboII GAAGA 5 cut(s) 101, 277, 343, 368, 392
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 3 cut(s) 146, 179, 248
MnlI CCTC 8 cut(s) 31, 64, 112, 196, 220, 255, 330, 492
Mph1103I ATGCAT 1 cut(s) 446
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 4 cut(s) 231, 302, 549, 559
MslI CAYNNNNRTG 2 cut(s) 114, 530
MspI CCGG 2 cut(s) 31, 257
MspR9I CCNGG 3 cut(s) 31, 257, 351
Mva1269I GAATGC 2 cut(s) 444, 446
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 40, 402
NciI CCSGG 2 cut(s) 31, 257
NdeII GATC 3 cut(s) 105, 151, 360
NlaIII CATG 2 cut(s) 428, 456
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 446
NspI RCATGY 1 cut(s) 428
PciI ACATGT 1 cut(s) 424
PctI GAATGC 2 cut(s) 444, 446
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 2 cut(s) 5, 268
PkrI GCNGC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 121
PscI ACATGT 1 cut(s) 424
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 349
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 2 cut(s) 121, 254
PspPPI RGGWCCY 1 cut(s) 121
PsuI RGATCY 1 cut(s) 360
RseI CAYNNNNRTG 2 cut(s) 114, 530
SaqAI TTAA 4 cut(s) 231, 302, 549, 559
SatI GCNGC 1 cut(s) 199
Sau3AI GATC 3 cut(s) 105, 151, 360
Sau96I GGNCC 2 cut(s) 121, 254
ScrFI CCNGG 3 cut(s) 31, 257, 351
SduI GDGCHC 1 cut(s) 210
SetI ASST 1 cut(s) 231
SfcI CTRYAG 2 cut(s) 364, 507
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 2 cut(s) 114, 530
Sse9I AATT 3 cut(s) 146, 179, 248
SsiI CCGC 3 cut(s) 34, 43, 398
SspI AATATT 1 cut(s) 219
StyD4I CCNGG 3 cut(s) 29, 255, 349
TaaI ACNGT 2 cut(s) 138, 168
TaqI TCGA 3 cut(s) 69, 150, 296
TasI AATT 3 cut(s) 146, 179, 248
TfiI GAWTC 2 cut(s) 5, 268
Tru1I TTAA 4 cut(s) 231, 302, 549, 559
Tru9I TTAA 4 cut(s) 231, 302, 549, 559
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 3 cut(s) 146, 422, 483
TspGWI ACGGA 2 cut(s) 46, 478
TspRI CASTG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 121
XapI RAATTY 2 cut(s) 146, 248
XceI RCATGY 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 444
Zsp2I ATGCAT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.