RLG00000001067

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018449
Physical Location & Seq
Forward (+)
10066675 .. 10067544
870 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001067

Sequence Viewer

Length: 870 bp
ATGGATTCGCAGGATGTGGTGGTCACTTCCCGGCGGGAGGAGCGGTTCAGCGCCTCTGTGGAACAAGTCGAACCCATCTACCCACCAGGTCTGGAACAAGCACCTTCACCCGCTCGACTTCAAGTCCACTTTGACTACATTACCCAGCACCAAATCTTCTTCAGCTCATGGTCTACCCGGAGCATATACTCCCTCCCATCATCACTGGAAGAAGACTGTCCAACCCTGATCCACCATTATGAGGACCCCGTGATGAAACTGTGTGGTGAATTCGATCTGCAAGTGCTACTGAGTACAGATGACGAACTGGGTGCAAATTTTTCACCGTTTCTTTCGCAATTTGAAATCACGCAAGGCAGCGAGGAGCACACCAGAATATTGGGTGAGATTAAGCGTGTGGGCAGGGAAATTGGTCCGGAGAGGAACAATTTAGGGTTATTTGCCTTGCAAGTTTCGATTTTAAGAGTATACACGACATATCGTTGCGAGAGTAATCCTGAGCGGGATGTAGAGGGGCAAGAGATGACCGACGTTATAGAAGAGGGAGAGATGCCGGTTTGGGACGTTATAGAGGGGGATGAGATGACTGACGTTATAGAAGAGGGAGAGATGCTGGTTTGGGACATTATAGAGGGGGAAGAGATGAGGGTTAGAGTTCGAGGGGAAGAGATCTGTAGTGTGTGCTTAGAGGAGTTTAGGAGTTGGCCGCCTTGCACTGCCATGCCTTGCTCACATGTGTTTCATAGCGAATGCATTCGGACATGGCTGGCACGGAATGAACGCTCCTGTCCTCTTTGCCGATTTCAGATGCCTACTATAGTTGAACCATCCTTCACATATATGTTCCATCTTGGGCTTAATCTTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

290

Amino Acids

33.34

Weight (kDa)

4.48

Isoelectric Point (pI)

70.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 222 - 267 4.3e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 225 - 267 4e-12 Ring finger domain
zf-C3HC4 PF00097 225 - 266 8.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 225 - 252 4.1e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 225 - 266 2.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 225 - 264 2.4e-07 RING-type zinc-finger
zf-RING_5 PF14634 225 - 267 2.1e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g35350 FvH4_5g35361
rosa_chinensis RchiOBHm_Chr7g0236701
rosa_laevigata RLG00000001067
rosa_multiflora Rmu_sc0002536.1_g000017
rosa_roxburghii Rroxscaffold_3G00224950
rosa_rugosa Rorug07G0298300
rosa_samantha Rh7AG453700 Rh7CG472200 Rh7DG441100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 43, 113, 502
AccI GTMKAC 2 cut(s) 173, 468
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 5 cut(s) 34, 43, 111, 502, 707
AclWI GGATC 1 cut(s) 223
AcoI YGGCCR 1 cut(s) 704
AcsI RAATTY 2 cut(s) 269, 316
AcuI CTGAAG 1 cut(s) 145
AfaI GTAC 1 cut(s) 295
AfiI CCNNNNNNNGG 2 cut(s) 37, 241
AflIII ACRYGT 1 cut(s) 733
AgsI TTSAA 3 cut(s) 122, 344, 824
AhdI GACNNNNNGTC 1 cut(s) 122
AjnI CCWGG 1 cut(s) 85
AloI GAACNNNNNNTCC 2 cut(s) 29, 61
AluBI AGCT 1 cut(s) 165
AluI AGCT 1 cut(s) 165
Alw21I GWGCWC 1 cut(s) 369
AlwI GGATC 1 cut(s) 223
Aor13HI TCCGGA 1 cut(s) 415
AoxI GGCC 1 cut(s) 704
ApeKI GCWGC 1 cut(s) 357
ApoI RAATTY 2 cut(s) 269, 316
Asp700I GAANNNNTTC 1 cut(s) 753
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 2 cut(s) 244, 413
AsuC2I CCSGG 2 cut(s) 31, 178
AsuHPI GGTGA 4 cut(s) 99, 278, 315, 395
AvaII GGWCC 2 cut(s) 244, 413
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 369
BccI CCATC 4 cut(s) 83, 205, 835, 855
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 87
BcnI CCSGG 2 cut(s) 31, 178
BfmI CTRYAG 2 cut(s) 673, 816
BfoI RGCGCY 1 cut(s) 54
BglII AGATCT 1 cut(s) 669
BisI GCNGC 2 cut(s) 358, 707
BlsI GCNGC 2 cut(s) 359, 708
Bme1390I CCNGG 3 cut(s) 31, 87, 178
Bme18I GGWCC 2 cut(s) 244, 413
BmeRI GACNNNNNGTC 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 244, 413
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 3 cut(s) 31, 87, 178
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 3 cut(s) 540, 600, 798
BmuI ACTGGG 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 498
BpuMI CCSGG 2 cut(s) 31, 178
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 241
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 2 cut(s) 210, 312
BseAI TCCGGA 1 cut(s) 415
BseBI CCWGG 1 cut(s) 87
BseGI GGATG 4 cut(s) 19, 511, 583, 827
BseLI CCNNNNNNNGG 2 cut(s) 37, 241
BseMII CTCAG 2 cut(s) 281, 489
BseNI ACTGG 2 cut(s) 210, 312
BseRI GAGGAG 3 cut(s) 53, 377, 704
BseXI GCAGC 1 cut(s) 369
BseYI CCCAGC 1 cut(s) 144
BshFI GGCC 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 4 cut(s) 31, 178, 416, 554
BslFI GGGAC 2 cut(s) 575, 635
BslI CCNNNNNNNGG 2 cut(s) 37, 241
BsmFI GGGAC 2 cut(s) 575, 635
BsmI GAATGC 2 cut(s) 753, 755
BsnI GGCC 1 cut(s) 706
Bsp1286I GDGCHC 1 cut(s) 369
Bsp13I TCCGGA 1 cut(s) 415
Bsp143I GATC 3 cut(s) 228, 274, 669
BspACI CCGC 5 cut(s) 34, 43, 111, 502, 707
BspANI GGCC 1 cut(s) 706
BspCNI CTCAG 2 cut(s) 282, 490
BspEI TCCGGA 1 cut(s) 415
BspLI GGNNCC 1 cut(s) 246
BspPI GGATC 1 cut(s) 223
BsrBI CCGCTC 3 cut(s) 43, 113, 502
BsrFI RCCGGY 1 cut(s) 553
BsrI ACTGG 2 cut(s) 210, 312
BssAI RCCGGY 1 cut(s) 553
BssMI GATC 3 cut(s) 228, 274, 669
BssNAI GTATAC 1 cut(s) 469
Bst1107I GTATAC 1 cut(s) 469
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 3 cut(s) 218, 261, 327
Bst6I CTCTTC 4 cut(s) 534, 594, 633, 660
BstC8I GCNNGC 1 cut(s) 768
BstDEI CTNAG 3 cut(s) 290, 498, 685
BstF5I GGATG 4 cut(s) 19, 511, 583, 827
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 3 cut(s) 231, 277, 672
BstMBI GATC 3 cut(s) 228, 274, 669
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 737
BstSCI CCNGG 3 cut(s) 29, 85, 176
BstSFI CTRYAG 2 cut(s) 673, 816
BstV1I GCAGC 1 cut(s) 369
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 669
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 1 cut(s) 669
BstZ17I GTATAC 1 cut(s) 469
BsuRI GGCC 1 cut(s) 706
BtsCI GGATG 4 cut(s) 19, 511, 583, 827
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 2 cut(s) 203, 714
Cac8I GCNNGC 1 cut(s) 768
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 553
Cfr13I GGNCC 2 cut(s) 244, 413
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 1 cut(s) 294
CviAII CATG 4 cut(s) 168, 721, 734, 762
CviJI RGCY 4 cut(s) 165, 706, 766, 856
CviKI_1 RGCY 4 cut(s) 165, 706, 766, 856
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 3 cut(s) 290, 498, 685
DpnI GATC 3 cut(s) 230, 276, 671
DpnII GATC 3 cut(s) 228, 274, 669
DriI GACNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 704
Eam1104I CTCTTC 4 cut(s) 534, 594, 633, 660
Eam1105I GACNNNNNGTC 1 cut(s) 122
EarI CTCTTC 4 cut(s) 534, 594, 633, 660
Eco47I GGWCC 2 cut(s) 244, 413
Eco57I CTGAAG 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 755
FaeI CATG 4 cut(s) 171, 724, 737, 765
FaqI GGGAC 2 cut(s) 575, 635
FatI CATG 4 cut(s) 167, 720, 733, 761
FauI CCCGC 3 cut(s) 27, 118, 495
FblI GTMKAC 2 cut(s) 173, 468
Fnu4HI GCNGC 2 cut(s) 358, 707
FokI GGATG 4 cut(s) 26, 518, 590, 814
Fsp4HI GCNGC 2 cut(s) 358, 707
GlaI GCGC 1 cut(s) 52
GluI GCNGC 2 cut(s) 358, 707
GsaI CCCAGC 1 cut(s) 148
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 706
HapII CCGG 4 cut(s) 31, 178, 416, 554
HhaI GCGC 1 cut(s) 53
Hin1II CATG 4 cut(s) 171, 724, 737, 765
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HinfI GANTC 1 cut(s) 5
HpaII CCGG 4 cut(s) 31, 178, 416, 554
HphI GGTGA 4 cut(s) 99, 278, 315, 395
Hpy166II GTNNAC 3 cut(s) 127, 174, 469
Hpy188I TCNGA 2 cut(s) 759, 807
Hpy188III TCNNGA 3 cut(s) 92, 416, 497
Hpy8I GTNNAC 3 cut(s) 127, 174, 469
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 2 cut(s) 114, 841
HpyCH4III ACNGT 3 cut(s) 218, 261, 327
HpyCH4IV ACGT 3 cut(s) 531, 564, 591
HpyCH4V TGCA 5 cut(s) 280, 314, 448, 714, 753
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 3 cut(s) 290, 498, 685
HpySE526I ACGT 3 cut(s) 531, 564, 591
Hsp92II CATG 4 cut(s) 171, 724, 737, 765
HspAI GCGC 1 cut(s) 51
Kpn2I TCCGGA 1 cut(s) 415
Kzo9I GATC 3 cut(s) 228, 274, 669
LmnI GCTCC 4 cut(s) 40, 180, 364, 788
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 3 cut(s) 540, 600, 798
MabI ACCWGGT 1 cut(s) 85
MaeII ACGT 3 cut(s) 531, 564, 591
MaeIII GTNAC 1 cut(s) 22
MalI GATC 3 cut(s) 230, 276, 671
MbiI CCGCTC 3 cut(s) 43, 113, 502
MboI GATC 3 cut(s) 228, 274, 669
MboII GAAGA 8 cut(s) 148, 151, 221, 224, 551, 611, 650, 677
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 5 cut(s) 269, 316, 338, 408, 427
MmeI TCCRAC 1 cut(s) 245
Mph1103I ATGCAT 1 cut(s) 755
MroI TCCGGA 1 cut(s) 415
MroXI GAANNNNTTC 1 cut(s) 753
MseI TTAA 4 cut(s) 390, 461, 858, 868
MslI CAYNNNNRTG 3 cut(s) 237, 719, 839
MspI CCGG 4 cut(s) 31, 178, 416, 554
MspR9I CCNGG 3 cut(s) 31, 87, 178
Mva1269I GAATGC 2 cut(s) 753, 755
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 40
NciI CCSGG 2 cut(s) 31, 178
NdeII GATC 3 cut(s) 228, 274, 669
NlaIII CATG 4 cut(s) 171, 724, 737, 765
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 755
NspI RCATGY 1 cut(s) 737
PciI ACATGT 1 cut(s) 733
PctI GAATGC 2 cut(s) 753, 755
PdmI GAANNNNTTC 1 cut(s) 753
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 2 cut(s) 359, 708
PpuMI RGGWCCY 1 cut(s) 244
PscI ACATGT 1 cut(s) 733
Psp5II RGGWCCY 1 cut(s) 244
Psp6I CCWGG 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 2 cut(s) 244, 413
PspPPI RGGWCCY 1 cut(s) 244
PsuI RGATCY 1 cut(s) 669
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
RseI CAYNNNNRTG 3 cut(s) 237, 719, 839
SaqAI TTAA 4 cut(s) 390, 461, 858, 868
SatI GCNGC 2 cut(s) 358, 707
Sau3AI GATC 3 cut(s) 228, 274, 669
Sau96I GGNCC 2 cut(s) 244, 413
ScrFI CCNGG 3 cut(s) 31, 87, 178
SduI GDGCHC 1 cut(s) 369
SetI ASST 6 cut(s) 91, 106, 167, 534, 567, 594
SexAI ACCWGGT 1 cut(s) 85
SfaNI GCATC 3 cut(s) 540, 600, 798
SfcI CTRYAG 2 cut(s) 673, 816
SinI GGWCC 2 cut(s) 244, 413
SmiMI CAYNNNNRTG 3 cut(s) 237, 719, 839
Sse9I AATT 5 cut(s) 269, 316, 338, 408, 427
SsiI CCGC 5 cut(s) 34, 43, 111, 502, 707
SspI AATATT 1 cut(s) 378
StyD4I CCNGG 3 cut(s) 29, 85, 176
TaaI ACNGT 3 cut(s) 218, 261, 327
TaiI ACGT 3 cut(s) 534, 567, 594
TaqI TCGA 5 cut(s) 69, 115, 273, 455, 658
TaqII GACCGA 1 cut(s) 542
TasI AATT 5 cut(s) 269, 316, 338, 408, 427
TatI WGTACW 1 cut(s) 293
TauI GCSGC 1 cut(s) 709
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 4 cut(s) 390, 461, 858, 868
Tru9I TTAA 4 cut(s) 390, 461, 858, 868
TscAI CASTG 2 cut(s) 210, 721
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 3 cut(s) 269, 731, 792
TspGWI ACGGA 1 cut(s) 787
TspRI CASTG 2 cut(s) 210, 721
VpaK11BI GGWCC 2 cut(s) 244, 413
XapI RAATTY 2 cut(s) 269, 316
XceI RCATGY 1 cut(s) 737
XmiI GTMKAC 2 cut(s) 173, 468
XmnI GAANNNNTTC 1 cut(s) 753
Zsp2I ATGCAT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.