Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0003097

Physical Location & Seq

Genomic Coordinates Forward (+)

25902366 .. 25908290

Gene Structure 5925 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g35370.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGCTGCAAGCTGGTTCTATGAGGTTGTTGTATGAATGGAGAGGTCCTAGTGTTCATGGATGCCATAATAATCCACATGCTGGTGTTGTTGGAAAGGGTCGAGTACATATGGTTAGCATCAATCTTCCGTCGCATGGCTTGGGTGCCTGCAGTCTCCATCTTCTAAGCCGTGTTCGTGGCCCAATAGGTCCAGTATCTTCCAGATGTAATGTTTTCTTGTGTAGATCTGTTTTTATACCAAGTGGAGGAACTGGGGCTCCCGCTCTGAAATCTGCTGCTATAGTTTTAACAAGGTCTTATAATGCTTTACGTGGAAGCACTGTCATGCTTAAATTGATTCAGGCAGTTGCTATCATTGCTTTTGCTGTTTGGGGTCTTGGACCGCTGATGCGCACGGGGAGGATTATATTTTTTCAACGAACTGATAGTAGTTGGAAGAAAAGTAGGTCACACTATGCTATGACCTCTTACATTCGGCCTTTAATGCTTTGGGGCGGAGCAATGCTTGTATGCAGAGCATTGGATCCTTTAGTCCTACCATCAGAAGCTAGCCAGGCTGTCAAGCAAAGACTTGTACATTTTTTACAATCAATATCAACAGTGCTGGCATTTGCCTACTGTTTGTCGAGCTTGAACCAACAAGCACAGAAATTCTTTGCGGAGACAAATGACCCAAGTGATACCAGAAATATGGGCTTTAGTTTTGCTGGCAAAGCTGTTTATTCGGCAGTTTGGGTTGCTGCAGTGTCATTGTTTATGGAGTTGCTGGGATTCTCAACCCAGAGATGGCTAACAGCTGGGGGTCTGGGTACAGTATTGCTCACGCTTGCTGGTCGTGAGATATTCACAAACTTCCTTTCCAGTGTAATGATCCATGCCACACGGCCATTTGTTGTCAGTGAATGGATTCAGACTAAGATTGAAGGCTATGAAGTTTCTGGTACTGTCGAGCATGTGGGGTGGTGGTCACCAACAATAATTAGAGGCGACGATCGGGAAGCAGTTCACATTCCAAATCACAAGTTCACTGTGAATGTTGTGAGGAATCTTAGCCAGAAGACTCATTGGCGTATCAAGAACTACATAGCCATTAGTCATTTGGATGTCAATAAAATTAATATTATTGTAGCTGACATGCGCAAGGTTTTAGCCAAAAATCCTCAAATAGAGCAGCAAAGGTTGCATAGAAGAGTGTTTCTGGATAATATCAATCCAGAGAATCAGGCTCTTATGATTTTGGTATCTTGCTTTGTGAAAACATCTCACATTGAGGAGTATCTCTGCGTGAAGGAGGCAATACTCTTGGATCTTCTCAGAGTTGTCAGCCATCATCGTGCTCGCCTTGCCACACCTCTCCGCACAGTTCAGAAAGTATACGGGGATGCTGACTTGGAAAATGTGCCATTTGCTGACACCATTTTTGGTAATTCTACAGCGCGTACTAATCGACCATATCTCCTGATCGAGCCATCATACAGAATTAGCAGTGATGATAAAAGTAAGGCTTCTAATCGCTCAGCACGCACAAATGGAGACAAGGAAGAAGCTAAACCTGGGCCAATACCAACTCCTACACAAACTGAAGATAAGGCAAGTTTTACTTCCAACTCAAGCACTAGTCCCAAGACTTCGGAAATGCCATCTGAACCCCAGACACAGAATGAGAAAACAGAATATGCAGGGAAGGAGAAGAAAGTTGATTCCAAATATGTATCTCCAGAAAAGGTGACATCTAAGAATTCTCCTGTTACTTCAAGTGAGATGGATGGTGGAAAGGCAGATATTCCTCTGACCACTGTACATGCAAAGCAAGATGGAGAGAAACCTGTTACATCTCCGTCTACAGCCCGCCCTCCCTTAGAAGAGAATATCATTCTCGGTGTTGCCTTAGAGGGATCTAAGCGAACACTTCCAATTGAGGAAGATATGGCTCCATCTCCTATAGAATCAAAAGAATTTACTGCGAGCCGTAATGGCAGTGGCGGGTCTCCTCTTGGCACAGATTTGAAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

672

Amino Acids

73.8

Weight (kDa)

9.44

Isoelectric Point (pI)

45.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	131 - 259	4.5e-68	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	261 - 331	6.5e-12	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	336 - 422	2.3e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58200 AT1G58200
fragaria_vesca	FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370
malus_domestica	MD08G1197800.v1.1
prunus_persica	Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1
pyrus_communis	pycom15g34530
rosa_chinensis	RchiOBHm_Chr7g0236721
rosa_laevigata	RLG00000001066
rosa_multiflora	Rmu_co8258783.1_g000001 Rmu_sc0003720.1_g000014 Rmu_sc0011298.1_g000003
rosa_roxburghii	Rroxscaffold_3G00224920
rosa_rugosa	Rorug07G0298400 Rorug07G0298500 Rorug07G0298600
rosa_samantha	Rh7AG454000 Rh7BG424700 Rh7CG472300 Rh7DG441300
rosa_wichuraiana	Rw7G037640 Rw7G037670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	300
Acc16I	TGCGCA	2 cut(s)	392, 1139
AccB1I	GGYRCC	1 cut(s)	143
AccB7I	CCANNNNNTGG	1 cut(s)	80
AccBSI	CCGCTC	1 cut(s)	263
AccI	GTMKAC	2 cut(s)	1374, 1841
AccII	CGCG	1 cut(s)	1438
AciI	CCGC	7 cut(s)	261, 383, 495, 659, 1357, 1849, 1983
AclWI	GGATC	5 cut(s)	518, 531, 865, 1314, 1903
AcoI	YGGCCR	1 cut(s)	884
AcsI	RAATTY	3 cut(s)	650, 1738, 1955
AcuI	CTGAAG	1 cut(s)	1602
AfaI	GTAC	6 cut(s)	105, 576, 811, 943, 1441, 1800
AfiI	CCNNNNNNNGG	4 cut(s)	80, 134, 245, 786
AgsI	TTSAA	5 cut(s)	416, 634, 923, 1755, 2008
AhlI	ACTAGT	1 cut(s)	1616
AjnI	CCWGG	2 cut(s)	552, 1552
AloI	GAACNNNNNNTCC	2 cut(s)	241, 273
AluBI	AGCT	7 cut(s)	11, 548, 630, 716, 797, 1130, 1547
AluI	AGCT	7 cut(s)	11, 548, 630, 716, 797, 1130, 1547
Alw21I	GWGCWC	1 cut(s)	1339
Alw26I	GTCTC	4 cut(s)	158, 656, 1527, 1992
AlwI	GGATC	5 cut(s)	518, 531, 865, 1314, 1903
AoxI	GGCC	4 cut(s)	178, 476, 884, 1556
ApeKI	GCWGC	4 cut(s)	4, 275, 740, 1171
ApoI	RAATTY	3 cut(s)	650, 1738, 1955
ArsI	GACNNNNNNTTYG	2 cut(s)	1403, 1435
AseI	ATTAAT	1 cut(s)	1116
Asp700I	GAANNNNTTC	2 cut(s)	906, 1002
AspLEI	GCGC	3 cut(s)	393, 1140, 1438
AspS9I	GGNCC	5 cut(s)	44, 179, 188, 380, 1556
AsuHPI	GGTGA	2 cut(s)	960, 1738
AsuNHI	GCTAGC	1 cut(s)	548
AvaII	GGWCC	3 cut(s)	44, 188, 380
BamHI	GGATCC	1 cut(s)	523
BanI	GGYRCC	1 cut(s)	143
BanII	GRGCYC	1 cut(s)	259
BbsI	GAAGAC	1 cut(s)	1064
Bbv12I	GWGCWC	1 cut(s)	1339
BbvI	GCAGC	3 cut(s)	262, 727, 1183
BceAI	ACGGC	3 cut(s)	153, 899, 1953
BciT130I	CCWGG	2 cut(s)	554, 1554
BcoDI	GTCTC	4 cut(s)	158, 656, 1527, 1992
BcuI	ACTAGT	1 cut(s)	1616
BfaI	CTAG	3 cut(s)	48, 549, 1617
BfmI	CTRYAG	6 cut(s)	148, 279, 741, 1431, 1842, 1941
BglI	GCCNNNNNGGC	1 cut(s)	1974
BglII	AGATCT	1 cut(s)	224
BisI	GCNGC	4 cut(s)	5, 276, 741, 1172
BlpI	GCTNAGC	1 cut(s)	1516
BlsI	GCNGC	4 cut(s)	6, 277, 742, 1173
Bme1390I	CCNGG	2 cut(s)	554, 1554
Bme18I	GGWCC	3 cut(s)	44, 188, 380
BmgT120I	GGNCC	5 cut(s)	44, 179, 188, 380, 1556
BmiI	GGNNCC	4 cut(s)	145, 258, 525, 1932
BmrFI	CCNGG	2 cut(s)	554, 1554
BmrI	ACTGGG	1 cut(s)	261
BmsI	GCATC	4 cut(s)	50, 126, 378, 1372
BmtI	GCTAGC	1 cut(s)	552
BmuI	ACTGGG	1 cut(s)	261
BpiI	GAAGAC	1 cut(s)	1064
BpmI	CTGGAG	1 cut(s)	1701
Bpu1102I	GCTNAGC	1 cut(s)	1516
BpuEI	CTTGAG	1 cut(s)	1594
BsaAI	YACGTR	1 cut(s)	311
BsaI	GGTCTC	1 cut(s)	1992
BsaJI	CCNNGG	1 cut(s)	1553
BsaXI	ACNNNNNCTCC	4 cut(s)	241, 271, 1440, 1470
Bsc4I	CCNNNNNNNGG	4 cut(s)	80, 134, 245, 786
Bse1I	ACTGG	3 cut(s)	191, 256, 861
Bse3DI	GCAATG	2 cut(s)	354, 507
BseBI	CCWGG	2 cut(s)	554, 1554
BseDI	CCNNGG	1 cut(s)	1553
BseGI	GGATG	4 cut(s)	65, 1108, 1387, 1771
BseLI	CCNNNNNNNGG	4 cut(s)	80, 134, 245, 786
BseMI	GCAATG	2 cut(s)	354, 507
BseMII	CTCAG	2 cut(s)	1327, 1530
BseNI	ACTGG	3 cut(s)	191, 256, 861
BseRI	GAGGAG	2 cut(s)	1286, 1980
BseXI	GCAGC	3 cut(s)	262, 727, 1183
BseYI	CCCAGC	2 cut(s)	766, 797
Bsh1236I	CGCG	1 cut(s)	1438
Bsh1285I	CGRYCG	1 cut(s)	994
BshFI	GGCC	4 cut(s)	180, 478, 886, 1558
BshNI	GGYRCC	1 cut(s)	143
BsiEI	CGRYCG	1 cut(s)	994
BsiHKAI	GWGCWC	1 cut(s)	1339
BslFI	GGGAC	1 cut(s)	1605
BslI	CCNNNNNNNGG	4 cut(s)	80, 134, 245, 786
BsmAI	GTCTC	4 cut(s)	158, 656, 1527, 1992
BsmFI	GGGAC	1 cut(s)	1605
BsnI	GGCC	4 cut(s)	180, 478, 886, 1558
Bso31I	GGTCTC	1 cut(s)	1992
Bsp1286I	GDGCHC	2 cut(s)	259, 1339
Bsp1407I	TGTACA	2 cut(s)	574, 1798
Bsp143I	GATC	7 cut(s)	224, 523, 870, 991, 1306, 1461, 1895
Bsp1720I	GCTNAGC	1 cut(s)	1516
BspACI	CCGC	7 cut(s)	261, 383, 495, 659, 1357, 1849, 1983
BspANI	GGCC	4 cut(s)	180, 478, 886, 1558
BspCNI	CTCAG	2 cut(s)	1326, 1529
BspFNI	CGCG	1 cut(s)	1438
BspLI	GGNNCC	4 cut(s)	145, 258, 525, 1932
BspMAI	CTGCAG	2 cut(s)	152, 745
BspOI	GCTAGC	1 cut(s)	552
BspPI	GGATC	5 cut(s)	518, 531, 865, 1314, 1903
BspT107I	GGYRCC	1 cut(s)	143
BspTNI	GGTCTC	1 cut(s)	1992
BsrBI	CCGCTC	1 cut(s)	263
BsrDI	GCAATG	2 cut(s)	354, 507
BsrGI	TGTACA	2 cut(s)	574, 1798
BsrI	ACTGG	3 cut(s)	191, 256, 861
BssECI	CCNNGG	1 cut(s)	1553
BssMI	GATC	7 cut(s)	224, 523, 870, 991, 1306, 1461, 1895
BssNAI	GTATAC	1 cut(s)	1375
Bst1107I	GTATAC	1 cut(s)	1375
Bst2UI	CCWGG	2 cut(s)	554, 1554
Bst4CI	ACNGT	8 cut(s)	322, 601, 620, 814, 946, 1030, 1363, 1798
Bst6I	CTCTTC	2 cut(s)	1183, 1857
BstAPI	GCANNNNNTGC	1 cut(s)	1180
BstAUI	TGTACA	2 cut(s)	574, 1798
BstBAI	YACGTR	1 cut(s)	311
BstDEI	CTNAG	9 cut(s)	164, 915, 1049, 1313, 1516, 1734, 1858, 1888, 1899
BstEII	GGTNACC	1 cut(s)	966
BstF5I	GGATG	4 cut(s)	65, 1108, 1387, 1771
BstFNI	CGCG	1 cut(s)	1438
BstHHI	GCGC	3 cut(s)	393, 1140, 1438
BstKTI	GATC	7 cut(s)	227, 526, 873, 994, 1309, 1464, 1898
BstMAI	GTCTC	4 cut(s)	158, 656, 1527, 1992
BstMBI	GATC	7 cut(s)	224, 523, 870, 991, 1306, 1461, 1895
BstMCI	CGRYCG	1 cut(s)	994
BstMWI	GCNNNNNNNGC	8 cut(s)	356, 484, 554, 713, 1180, 1343, 1521, 1974
BstNI	CCWGG	2 cut(s)	554, 1554
BstNSI	RCATGY	4 cut(s)	80, 956, 1138, 1805
BstPI	GGTNACC	1 cut(s)	966
BstSCI	CCNGG	2 cut(s)	552, 1552
BstSFI	CTRYAG	6 cut(s)	148, 279, 741, 1431, 1842, 1941
BstUI	CGCG	1 cut(s)	1438
BstV1I	GCAGC	3 cut(s)	262, 727, 1183
BstV2I	GAAGAC	1 cut(s)	1064
BstX2I	RGATCY	4 cut(s)	224, 523, 1306, 1895
BstXI	CCANNNNNNTGG	1 cut(s)	691
BstYI	RGATCY	4 cut(s)	224, 523, 1306, 1895
BstZ17I	GTATAC	1 cut(s)	1375
BsuRI	GGCC	4 cut(s)	180, 478, 886, 1558
BtsCI	GGATG	4 cut(s)	65, 1108, 1387, 1771
BtsI	GCAGTG	3 cut(s)	750, 1492, 1984
BtsIMutI	CAGTG	9 cut(s)	318, 606, 750, 868, 904, 1026, 1492, 1794, 1984
CfoI	GCGC	3 cut(s)	393, 1140, 1438
Cfr13I	GGNCC	5 cut(s)	44, 179, 188, 380, 1556
Csp6I	GTAC	6 cut(s)	104, 575, 810, 942, 1440, 1799
CviAII	CATG	8 cut(s)	56, 77, 134, 325, 875, 953, 1135, 1802
CviQI	GTAC	6 cut(s)	104, 575, 810, 942, 1440, 1799
DdeI	CTNAG	9 cut(s)	164, 915, 1049, 1313, 1516, 1734, 1858, 1888, 1899
DpnI	GATC	7 cut(s)	226, 525, 872, 993, 1308, 1463, 1897
DpnII	GATC	7 cut(s)	224, 523, 870, 991, 1306, 1461, 1895
EaeI	YGGCCR	1 cut(s)	884
Eam1104I	CTCTTC	2 cut(s)	1183, 1857
EarI	CTCTTC	2 cut(s)	1183, 1857
EciI	GGCGGA	1 cut(s)	510
Eco24I	GRGCYC	1 cut(s)	259
Eco31I	GGTCTC	1 cut(s)	1992
Eco47I	GGWCC	3 cut(s)	44, 188, 380
Eco57I	CTGAAG	1 cut(s)	1602
Eco91I	GGTNACC	1 cut(s)	966
EcoO109I	RGGNCCY	1 cut(s)	44
EcoO65I	GGTNACC	1 cut(s)	966
EcoRI	GAATTC	1 cut(s)	1738
EcoRII	CCWGG	2 cut(s)	552, 1552
EcoT38I	GRGCYC	1 cut(s)	259
FaeI	CATG	8 cut(s)	59, 80, 137, 328, 878, 956, 1138, 1805
FalI	AAGNNNNNCTT	4 cut(s)	1489, 1521, 1585, 1617
FaqI	GGGAC	1 cut(s)	1605
FatI	CATG	8 cut(s)	55, 76, 133, 324, 874, 952, 1134, 1801
FauI	CCCGC	3 cut(s)	268, 1856, 1976
FauNDI	CATATG	1 cut(s)	108
FblI	GTMKAC	2 cut(s)	1374, 1841
Fnu4HI	GCNGC	4 cut(s)	5, 276, 741, 1172
FokI	GGATG	4 cut(s)	72, 1115, 1394, 1778
FriOI	GRGCYC	1 cut(s)	259
Fsp4HI	GCNGC	4 cut(s)	5, 276, 741, 1172
FspAI	RTGCGCAY	1 cut(s)	392
FspBI	CTAG	3 cut(s)	48, 549, 1617
FspI	TGCGCA	2 cut(s)	392, 1139
GlaI	GCGC	3 cut(s)	392, 1139, 1437
GluI	GCNGC	4 cut(s)	5, 276, 741, 1172
GsaI	CCCAGC	2 cut(s)	770, 801
GsuI	CTGGAG	1 cut(s)	1701
HaeIII	GGCC	4 cut(s)	180, 478, 886, 1558
HhaI	GCGC	3 cut(s)	393, 1140, 1438
Hin1II	CATG	8 cut(s)	59, 80, 137, 328, 878, 956, 1138, 1805
Hin6I	GCGC	3 cut(s)	391, 1138, 1436
HinP1I	GCGC	3 cut(s)	391, 1138, 1436
HinfI	GANTC	8 cut(s)	337, 771, 907, 1045, 1060, 1219, 1700, 1946
HphI	GGTGA	2 cut(s)	960, 1738
Hpy166II	GTNNAC	4 cut(s)	1006, 1026, 1375, 1842
Hpy188I	TCNGA	8 cut(s)	267, 544, 912, 1316, 1368, 1633, 1645, 1791
Hpy188III	TCNNGA	8 cut(s)	201, 836, 995, 1075, 1199, 1214, 1459, 1718
Hpy8I	GTNNAC	4 cut(s)	1006, 1026, 1375, 1842
Hpy99I	CGWCG	2 cut(s)	133, 992
HpyAV	CCTTC	3 cut(s)	917, 1282, 1678
HpyCH4III	ACNGT	8 cut(s)	322, 601, 620, 814, 946, 1030, 1363, 1798
HpyCH4IV	ACGT	1 cut(s)	310
HpyCH4V	TGCA	7 cut(s)	7, 150, 513, 743, 1183, 1679, 1805
HpyF10VI	GCNNNNNNNGC	8 cut(s)	356, 484, 554, 713, 1180, 1343, 1521, 1974
HpyF3I	CTNAG	9 cut(s)	164, 915, 1049, 1313, 1516, 1734, 1858, 1888, 1899
HpySE526I	ACGT	1 cut(s)	310
Hsp92II	CATG	8 cut(s)	59, 80, 137, 328, 878, 956, 1138, 1805
HspAI	GCGC	3 cut(s)	391, 1138, 1436
Kzo9I	GATC	7 cut(s)	224, 523, 870, 991, 1306, 1461, 1895
LmnI	GCTCC	3 cut(s)	262, 497, 1936
Lsp1109I	GCAGC	3 cut(s)	262, 727, 1183
LweI	GCATC	4 cut(s)	50, 126, 378, 1372
MaeI	CTAG	3 cut(s)	48, 549, 1617
MaeII	ACGT	1 cut(s)	310
MaeIII	GTNAC	5 cut(s)	447, 966, 1726, 1747, 1828
MalI	GATC	7 cut(s)	226, 525, 872, 993, 1308, 1463, 1897
MbiI	CCGCTC	1 cut(s)	263
MboI	GATC	7 cut(s)	224, 523, 870, 991, 1306, 1461, 1895
MfeI	CAATTG	1 cut(s)	1914
MflI	RGATCY	4 cut(s)	224, 523, 1306, 1895
MhlI	GDGCHC	2 cut(s)	259, 1339
MluCI	AATT	9 cut(s)	332, 650, 978, 1113, 1426, 1479, 1738, 1914, 1955
MlyI	GAGTC	1 cut(s)	1054
MmeI	TCCRAC	3 cut(s)	70, 413, 1629
MroXI	GAANNNNTTC	2 cut(s)	906, 1002
MseI	TTAA	4 cut(s)	287, 330, 482, 1116
MslI	CAYNNNNRTG	4 cut(s)	81, 323, 1101, 1332
MspA1I	CMGCKG	2 cut(s)	385, 797
MspR9I	CCNGG	2 cut(s)	554, 1554
MunI	CAATTG	1 cut(s)	1914
MvaI	CCWGG	2 cut(s)	554, 1554
MvnI	CGCG	1 cut(s)	1438
MwoI	GCNNNNNNNGC	8 cut(s)	356, 484, 554, 713, 1180, 1343, 1521, 1974
NdeI	CATATG	1 cut(s)	108
NdeII	GATC	7 cut(s)	224, 523, 870, 991, 1306, 1461, 1895
NheI	GCTAGC	1 cut(s)	548
NlaIII	CATG	8 cut(s)	59, 80, 137, 328, 878, 956, 1138, 1805
NlaIV	GGNNCC	4 cut(s)	145, 258, 525, 1932
NmuCI	GTSAC	3 cut(s)	447, 966, 1726
NsbI	TGCGCA	2 cut(s)	392, 1139
NspI	RCATGY	4 cut(s)	80, 956, 1138, 1805
PdmI	GAANNNNTTC	2 cut(s)	906, 1002
PfeI	GAWTC	7 cut(s)	337, 771, 907, 1045, 1219, 1700, 1946
PflMI	CCANNNNNTGG	1 cut(s)	80
PkrI	GCNGC	4 cut(s)	6, 277, 742, 1173
Ple19I	CGATCG	1 cut(s)	994
PleI	GAGTC	1 cut(s)	1054
PpsI	GAGTC	1 cut(s)	1054
Ppu21I	YACGTR	1 cut(s)	311
PpuMI	RGGWCCY	1 cut(s)	44
PshBI	ATTAAT	1 cut(s)	1116
PsiI	TTATAA	1 cut(s)	300
Psp5II	RGGWCCY	1 cut(s)	44
Psp6I	CCWGG	2 cut(s)	552, 1552
PspEI	GGTNACC	1 cut(s)	966
PspFI	CCCAGC	2 cut(s)	766, 797
PspGI	CCWGG	2 cut(s)	552, 1552
PspN4I	GGNNCC	4 cut(s)	145, 258, 525, 1932
PspPI	GGNCC	5 cut(s)	44, 179, 188, 380, 1556
PspPPI	RGGWCCY	1 cut(s)	44
PstI	CTGCAG	2 cut(s)	152, 745
PsuI	RGATCY	4 cut(s)	224, 523, 1306, 1895
PvuI	CGATCG	1 cut(s)	994
PvuII	CAGCTG	1 cut(s)	797
RsaI	GTAC	6 cut(s)	105, 576, 811, 943, 1441, 1800
RsaNI	GTAC	6 cut(s)	104, 575, 810, 942, 1440, 1799
RseI	CAYNNNNRTG	4 cut(s)	81, 323, 1101, 1332
SaqAI	TTAA	4 cut(s)	287, 330, 482, 1116
SatI	GCNGC	4 cut(s)	5, 276, 741, 1172
Sau3AI	GATC	7 cut(s)	224, 523, 870, 991, 1306, 1461, 1895
Sau96I	GGNCC	5 cut(s)	44, 179, 188, 380, 1556
SchI	GAGTC	1 cut(s)	1054
ScrFI	CCNGG	2 cut(s)	554, 1554
SduI	GDGCHC	2 cut(s)	259, 1339
SfaNI	GCATC	4 cut(s)	50, 126, 378, 1372
SfcI	CTRYAG	6 cut(s)	148, 279, 741, 1431, 1842, 1941
SinI	GGWCC	3 cut(s)	44, 188, 380
SmiMI	CAYNNNNRTG	4 cut(s)	81, 323, 1101, 1332
SmlI	CTYRAG	1 cut(s)	1609
SmoI	CTYRAG	1 cut(s)	1609
SpeI	ACTAGT	1 cut(s)	1616
Sse9I	AATT	9 cut(s)	332, 650, 978, 1113, 1426, 1479, 1738, 1914, 1955
SsiI	CCGC	7 cut(s)	261, 383, 495, 659, 1357, 1849, 1983
SspI	AATATT	1 cut(s)	1120
SspMI	CTAG	3 cut(s)	48, 549, 1617
StyD4I	CCNGG	2 cut(s)	552, 1552
TaaI	ACNGT	8 cut(s)	322, 601, 620, 814, 946, 1030, 1363, 1798
TaiI	ACGT	1 cut(s)	313
TaqI	TCGA	5 cut(s)	100, 626, 948, 1447, 1464
TasI	AATT	9 cut(s)	332, 650, 978, 1113, 1426, 1479, 1738, 1914, 1955
TatI	WGTACW	3 cut(s)	103, 574, 1798
TfiI	GAWTC	7 cut(s)	337, 771, 907, 1045, 1219, 1700, 1946
Tru1I	TTAA	4 cut(s)	287, 330, 482, 1116
Tru9I	TTAA	4 cut(s)	287, 330, 482, 1116
TscAI	CASTG	9 cut(s)	325, 606, 750, 868, 904, 1033, 1492, 1801, 1984
TseFI	GTSAC	3 cut(s)	447, 966, 1726
TseI	GCWGC	4 cut(s)	4, 275, 740, 1171
Tsp45I	GTSAC	3 cut(s)	447, 966, 1726
TspDTI	ATGAA	3 cut(s)	44, 48, 945
TspGWI	ACGGA	2 cut(s)	117, 1827
TspRI	CASTG	9 cut(s)	325, 606, 750, 868, 904, 1033, 1492, 1801, 1984
Van91I	CCANNNNNTGG	1 cut(s)	80
VpaK11BI	GGWCC	3 cut(s)	44, 188, 380
VspI	ATTAAT	1 cut(s)	1116
XapI	RAATTY	3 cut(s)	650, 1738, 1955
XceI	RCATGY	4 cut(s)	80, 956, 1138, 1805
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1096
XmiI	GTMKAC	2 cut(s)	1374, 1841
XmnI	GAANNNNTTC	2 cut(s)	906, 1002
XspI	CTAG	3 cut(s)	48, 549, 1617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.