Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003097

Physical Location & Seq

Genomic Coordinates Reverse (-)

10047005 .. 10053742

Gene Structure 6738 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001066

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2217 bp

ATGGATAATCCAGACTCGGAAATGAAAGAATTGGAACTGAGACTGATTGGTCCACGCTGTCCCCGTCAGAACCCACCCGTAACCTCCCGGCGCACGTTAGCGCAACGCCAGCCATTTCGGGCTTCTCCACACCTCTTTCTCACACGCCAGCGTCCAGTTCACGAAAGTTTCAATCTTCAGAAGAATTATCATCGTCTTCTCTCCTTTGGAGGTGTTGCACTGATTTGGGAGTCTGAGTCTTGGAGAACCGTGCCGGGTTCGAAAGCATTGTGGAGAGAGTTTGAGAATCAAGAAATGCCTCAAGCTGTTTCTATGAGGTTGTTGTATGAATGGAGAAGTCACAGTATTCATGGATGCCATAAACAGCCACATGCTTGTGCTGTAGGAAAGGGTCGAGTACATATGGTTAGCATCAATCTACCGTCACATGGCTTGGGTGCCTGCAGTCTCCATCTTTTAAGCAGTGTACGTGGCCCAATAGGTCCATTATCTTCCAGATGTAAAGTTTTCTTGTGTAGATCTGTTTTTATACCAAGTGGAGGAACTGGGGCTCCTGCTCTGAAATCTACTGCTGTAGTTTTAACAAGGTCTTATGATGCTTTACGTGGAAGCCCTGTTGTCCTTAAATTGATTCAGGCAGTTGCTGTCATTGCTTTTGCTGTTTGGGGTCTTGGGCCCCTGATGCGCATGGGGAGGATTATATTTTTTCAACGGACTGATAGTAGTTGGAAGAAAAGTAGGTCACACTATGCTATGACCTCTTGCATTCAGCCTTTGCTGCTTTGGGGTGGAGCAATGCTTGTATGCAGAGCATTGGATCCTGTAGTTCTACCATCAGAAGCTAGCCAGGCTGTCAAGCAAAGACTTATAAATTTTTCACAATCATTATCAACAGTGCTGGCGTTTGCCTACTGTTTGTCAAGCTTGAACCAACAAGCACAGAAATTCTTTGCGGAGACAAGTGACCCGAGTGATACCAGAAATATATTCACAAACTTCCTTTCCAGTGTAATGATCCATGCCACACGGCCATTTGTTGTCAATGAATGGATTCAGACTAAGATTCAAGGCTATGAAGTTTCCGGTACCGTCGAGCATGTGGGGTGGTGGTCACCAACAATTGTAAGAGGTGATGATCGTGAAGCAGTTCACATTCCAAATCACAAGTTCACTGTGAATGTTGTGAGGAATCTTAGCCAGAAGACTCATTGGCGCATCAAGAACCACATAGCCATTAGTCATTTGGATGTCAATAAAATTAATATTATTGTAGCTGATATGCGCAAGGTTTTAGCCAAAAATCCTCAAGTAGAGCAGCAAAGGTTGCATAGAAGAGTTTTTCTGGATAATATTAATCCAGAGAATCAGGCTCTTATGATTTTGGTATCTTGCTTTGTGAAAACATCTCACATTGAGGAGTATCTCTGCGTGAAGGAGGCAATACTCTTGGATCTTCTCAGAGTTGTCAGCCATCATCGTGCTCGGCTTGCCACACCCCTCCGCACAGTTCAGAAAGTATACGGTGATGCTGACATGGAAAATGTGCCATTTGCTGACACCATTTTTGGTAGTTCTACGGCACATACTAATCATCCATATCTCCTCATTGAGCCATCTTACAGAATCAGTAGTGATGACAAAAGTAAGGCTTCTAATCGCTCAGCACGAACAAATGGAGACAAGGAAGAACCTAAAGCTGGGCCAACACTAACACCTGCGCAAACTGAAGATAAGGCAACTTCAACTTCCAACTCAAGTACAAGTCCCAAGACTTCGGAAATGCCAACATCTGAACCCGAAACACAGAATTTGGCATCTGATGGTTCAGCCCCAGACACCTCTGAAATACTGCAGTCCAAGAATGAGACGATAAGATATGCAGGGAAGGAGAAGGGAGTTGATTCCAAAAATGTATCTCCAGAAAAGACAACATCTAAGAAGTCTCCTGTTACTTCATCTGAGATGGGTGGTGGAAAGACGGATATTCCTTTGACAACTTTACAGGCAAATCAAGATGGAGAGAAACCTGTTGCATCACCGTCTACAGCCCGCGCTCCCTTAGAAGAGAATATCATTCTCGGTGTTGCCTTAGAGGGCTCAAAGCGGACACTTCCCATTGAGGAAGATATGGGTTCAACCCTTATAGAATCAAAAGAATTCACCGCCAGCCGTAATGGCAGCGGCGGGTCTCCTCTTGGCACAGATGTGAAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

739

Amino Acids

81.62

Weight (kDa)

9.31

Isoelectric Point (pI)

53.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	249 - 339	4.1e-34	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	328 - 399	1.1e-11	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	404 - 490	1.9e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58200 AT1G58200
fragaria_vesca	FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370
malus_domestica	MD08G1197800.v1.1
prunus_persica	Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1
pyrus_communis	pycom15g34530
rosa_chinensis	RchiOBHm_Chr7g0236721
rosa_laevigata	RLG00000001066
rosa_multiflora	Rmu_co8258783.1_g000001 Rmu_sc0003720.1_g000014 Rmu_sc0011298.1_g000003
rosa_roxburghii	Rroxscaffold_3G00224920
rosa_rugosa	Rorug07G0298400 Rorug07G0298500 Rorug07G0298600
rosa_samantha	Rh7AG454000 Rh7BG424700 Rh7CG472300 Rh7DG441300
rosa_wichuraiana	Rw7G037640 Rw7G037670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	869
AarI	CACCTGC	1 cut(s)	1723
AasI	GACNNNNNNGTC	1 cut(s)	48
Acc16I	TGCGCA	3 cut(s)	686, 1283, 1719
Acc36I	ACCTGC	1 cut(s)	1723
Acc65I	GGTACC	1 cut(s)	1085
AccB1I	GGYRCC	2 cut(s)	437, 1085
AccI	GTMKAC	2 cut(s)	1518, 2042
AccII	CGCG	1 cut(s)	2052
AciI	CCGC	7 cut(s)	953, 1501, 2050, 2104, 2163, 2181, 2184
AclWI	GGATC	4 cut(s)	812, 825, 1009, 1458
AcoI	YGGCCR	1 cut(s)	1028
AcsI	RAATTY	4 cut(s)	871, 944, 1807, 2156
AcuI	CTGAAG	2 cut(s)	161, 1746
AfaI	GTAC	4 cut(s)	399, 468, 1087, 1759
AfiI	CCNNNNNNNGG	3 cut(s)	428, 539, 1697
AgsI	TTSAA	6 cut(s)	172, 710, 928, 1067, 1743, 2136
AjnI	CCWGG	1 cut(s)	846
AleI	CACNNNNGTG	1 cut(s)	2204
AloI	GAACNNNNNNTCC	2 cut(s)	535, 567
AluBI	AGCT	5 cut(s)	305, 842, 924, 1274, 1697
AluI	AGCT	5 cut(s)	305, 842, 924, 1274, 1697
Alw21I	GWGCWC	1 cut(s)	1483
Alw26I	GTCTC	7 cut(s)	34, 452, 950, 1671, 1859, 1947, 2193
AlwI	GGATC	4 cut(s)	812, 825, 1009, 1458
AlwNI	CAGNNNCTG	1 cut(s)	644
Ama87I	CYCGRG	1 cut(s)	967
AoxI	GGCC	4 cut(s)	472, 674, 1028, 1700
ApaI	GGGCCC	1 cut(s)	678
ApeKI	GCWGC	3 cut(s)	778, 1315, 2178
ApoI	RAATTY	4 cut(s)	871, 944, 1807, 2156
ArsI	GACNNNNNNTTYG	2 cut(s)	1547, 1579
AseI	ATTAAT	2 cut(s)	1260, 1353
Asp700I	GAANNNNTTC	2 cut(s)	1050, 1146
Asp718I	GGTACC	1 cut(s)	1085
AspLEI	GCGC	7 cut(s)	93, 103, 687, 1215, 1284, 1720, 2054
AspS9I	GGNCC	6 cut(s)	50, 473, 482, 674, 675, 1700
AsuC2I	CCSGG	2 cut(s)	88, 255
AsuHPI	GGTGA	5 cut(s)	1104, 1142, 1535, 2028, 2152
AsuII	TTCGAA	1 cut(s)	260
AsuNHI	GCTAGC	1 cut(s)	842
AvaI	CYCGRG	1 cut(s)	967
AvaII	GGWCC	2 cut(s)	50, 482
BaeGI	GKGCMC	1 cut(s)	678
BamHI	GGATCC	1 cut(s)	817
BanI	GGYRCC	2 cut(s)	437, 1085
BanII	GRGCYC	3 cut(s)	553, 678, 2099
BbsI	GAAGAC	2 cut(s)	188, 1208
Bbv12I	GWGCWC	1 cut(s)	1483
BbvI	GCAGC	3 cut(s)	765, 1327, 2190
BccI	CCATC	7 cut(s)	459, 841, 1479, 1621, 1814, 1957, 2009
BceAI	ACGGC	3 cut(s)	1043, 1593, 2154
BciT130I	CCWGG	1 cut(s)	848
BcnI	CCSGG	2 cut(s)	88, 255
BcoDI	GTCTC	7 cut(s)	34, 452, 950, 1671, 1859, 1947, 2193
BfaI	CTAG	1 cut(s)	843
BfmI	CTRYAG	6 cut(s)	381, 442, 573, 822, 1850, 2043
BfuAI	ACCTGC	1 cut(s)	1723
BglI	GCCNNNNNGGC	1 cut(s)	2175
BglII	AGATCT	1 cut(s)	518
BisI	GCNGC	4 cut(s)	779, 1316, 2179, 2182
BlpI	GCTNAGC	1 cut(s)	1660
BlsI	GCNGC	4 cut(s)	780, 1317, 2180, 2183
Bme1390I	CCNGG	3 cut(s)	88, 255, 848
Bme18I	GGWCC	2 cut(s)	50, 482
BmeT110I	CYCGRG	1 cut(s)	967
BmgT120I	GGNCC	6 cut(s)	50, 473, 482, 674, 675, 1700
BmiI	GGNNCC	6 cut(s)	439, 552, 676, 677, 819, 1087
BmrFI	CCNGG	3 cut(s)	88, 255, 848
BmrI	ACTGGG	1 cut(s)	555
BmsI	GCATC	8 cut(s)	344, 420, 586, 672, 1224, 1516, 1823, 2042
BmtI	GCTAGC	1 cut(s)	846
BmuI	ACTGGG	1 cut(s)	555
BpiI	GAAGAC	2 cut(s)	188, 1208
BpmI	CTGGAG	1 cut(s)	1902
Bpu1102I	GCTNAGC	1 cut(s)	1660
Bpu14I	TTCGAA	1 cut(s)	260
BpuEI	CTTGAG	3 cut(s)	285, 1290, 1738
BpuMI	CCSGG	2 cut(s)	88, 255
BsaAI	YACGTR	2 cut(s)	470, 605
BsaI	GGTCTC	1 cut(s)	2193
BsaWI	WCCGGW	1 cut(s)	1082
BsaXI	ACNNNNNCTCC	4 cut(s)	221, 251, 535, 565
Bsc4I	CCNNNNNNNGG	3 cut(s)	428, 539, 1697
Bse1I	ACTGG	3 cut(s)	155, 550, 1005
Bse3DI	GCAATG	2 cut(s)	648, 801
BseBI	CCWGG	1 cut(s)	848
BseGI	GGATG	3 cut(s)	359, 1252, 1591
BseLI	CCNNNNNNNGG	3 cut(s)	428, 539, 1697
BseMI	GCAATG	2 cut(s)	648, 801
BseMII	CTCAG	5 cut(s)	29, 225, 1471, 1674, 1950
BseNI	ACTGG	3 cut(s)	155, 550, 1005
BseRI	GAGGAG	3 cut(s)	1430, 1592, 2181
BseSI	GKGCMC	1 cut(s)	678
BseXI	GCAGC	3 cut(s)	765, 1327, 2190
BseYI	CCCAGC	1 cut(s)	1697
Bsh1236I	CGCG	1 cut(s)	2052
BshFI	GGCC	4 cut(s)	474, 676, 1030, 1702
BshNI	GGYRCC	2 cut(s)	437, 1085
BsiHKAI	GWGCWC	1 cut(s)	1483
BsiHKCI	CYCGRG	1 cut(s)	967
BsiSI	CCGG	3 cut(s)	88, 254, 1083
BslFI	GGGAC	2 cut(s)	45, 1749
BslI	CCNNNNNNNGG	3 cut(s)	428, 539, 1697
BsmAI	GTCTC	7 cut(s)	34, 452, 950, 1671, 1859, 1947, 2193
BsmBI	CGTCTC	1 cut(s)	1859
BsmFI	GGGAC	2 cut(s)	45, 1749
BsmI	GAATGC	1 cut(s)	765
BsnI	GGCC	4 cut(s)	474, 676, 1030, 1702
Bso31I	GGTCTC	1 cut(s)	2193
BsoBI	CYCGRG	1 cut(s)	967
Bsp119I	TTCGAA	1 cut(s)	260
Bsp120I	GGGCCC	1 cut(s)	674
Bsp1286I	GDGCHC	4 cut(s)	553, 678, 1483, 2099
Bsp143I	GATC	5 cut(s)	518, 817, 1014, 1135, 1450
Bsp1720I	GCTNAGC	1 cut(s)	1660
BspACI	CCGC	7 cut(s)	953, 1501, 2050, 2104, 2163, 2181, 2184
BspANI	GGCC	4 cut(s)	474, 676, 1030, 1702
BspCNI	CTCAG	5 cut(s)	30, 226, 1470, 1673, 1951
BspFNI	CGCG	1 cut(s)	2052
BspLI	GGNNCC	6 cut(s)	439, 552, 676, 677, 819, 1087
BspMAI	CTGCAG	2 cut(s)	446, 1854
BspMI	ACCTGC	1 cut(s)	1723
BspOI	GCTAGC	1 cut(s)	846
BspPI	GGATC	4 cut(s)	812, 825, 1009, 1458
BspT104I	TTCGAA	1 cut(s)	260
BspT107I	GGYRCC	2 cut(s)	437, 1085
BspTNI	GGTCTC	1 cut(s)	2193
BsrDI	GCAATG	2 cut(s)	648, 801
BsrI	ACTGG	3 cut(s)	155, 550, 1005
BssMI	GATC	5 cut(s)	518, 817, 1014, 1135, 1450
BssNAI	GTATAC	1 cut(s)	1519
Bst1107I	GTATAC	1 cut(s)	1519
Bst2UI	CCWGG	1 cut(s)	848
Bst6I	CTCTTC	2 cut(s)	1327, 2058
BstAPI	GCANNNNNTGC	1 cut(s)	1324
BstBAI	YACGTR	2 cut(s)	470, 605
BstBI	TTCGAA	1 cut(s)	260
BstC8I	GCNNGC	8 cut(s)	110, 149, 442, 844, 900, 1488, 2050, 2167
BstEII	GGTNACC	1 cut(s)	1110
BstF5I	GGATG	3 cut(s)	359, 1252, 1591
BstFNI	CGCG	1 cut(s)	2052
BstHHI	GCGC	7 cut(s)	93, 103, 687, 1215, 1284, 1720, 2054
BstKTI	GATC	5 cut(s)	521, 820, 1017, 1138, 1453
BstMAI	GTCTC	7 cut(s)	34, 452, 950, 1671, 1859, 1947, 2193
BstMBI	GATC	5 cut(s)	518, 817, 1014, 1135, 1450
BstMWI	GCNNNNNNNGC	8 cut(s)	109, 650, 682, 778, 848, 1324, 1487, 2175
BstNI	CCWGG	1 cut(s)	848
BstNSI	RCATGY	2 cut(s)	374, 1100
BstPI	GGTNACC	1 cut(s)	1110
BstSCI	CCNGG	3 cut(s)	86, 253, 846
BstSFI	CTRYAG	6 cut(s)	381, 442, 573, 822, 1850, 2043
BstSLI	GKGCMC	1 cut(s)	678
BstUI	CGCG	1 cut(s)	2052
BstV1I	GCAGC	3 cut(s)	765, 1327, 2190
BstV2I	GAAGAC	2 cut(s)	188, 1208
BstX2I	RGATCY	3 cut(s)	518, 817, 1450
BstYI	RGATCY	3 cut(s)	518, 817, 1450
BstZ17I	GTATAC	1 cut(s)	1519
BsuRI	GGCC	4 cut(s)	474, 676, 1030, 1702
BtsCI	GGATG	3 cut(s)	359, 1252, 1591
BtsI	GCAGTG	1 cut(s)	469
BtsIMutI	CAGTG	5 cut(s)	218, 469, 900, 1012, 1170
BveI	ACCTGC	1 cut(s)	1723
Cac8I	GCNNGC	8 cut(s)	110, 149, 442, 844, 900, 1488, 2050, 2167
CaiI	CAGNNNCTG	1 cut(s)	644
CfoI	GCGC	7 cut(s)	93, 103, 687, 1215, 1284, 1720, 2054
Cfr13I	GGNCC	6 cut(s)	50, 473, 482, 674, 675, 1700
CseI	GACGC	1 cut(s)	140
Csp6I	GTAC	4 cut(s)	398, 467, 1086, 1758
CviAII	CATG	7 cut(s)	350, 371, 428, 688, 1019, 1097, 1534
CviQI	GTAC	4 cut(s)	398, 467, 1086, 1758
DpnI	GATC	5 cut(s)	520, 819, 1016, 1137, 1452
DpnII	GATC	5 cut(s)	518, 817, 1014, 1135, 1450
DrdI	GACNNNNNNGTC	1 cut(s)	48
DseDI	GACNNNNNNGTC	1 cut(s)	48
EaeI	YGGCCR	1 cut(s)	1028
Eam1104I	CTCTTC	2 cut(s)	1327, 2058
EarI	CTCTTC	2 cut(s)	1327, 2058
Eco24I	GRGCYC	3 cut(s)	553, 678, 2099
Eco31I	GGTCTC	1 cut(s)	2193
Eco47I	GGWCC	2 cut(s)	50, 482
Eco57I	CTGAAG	2 cut(s)	161, 1746
Eco88I	CYCGRG	1 cut(s)	967
Eco91I	GGTNACC	1 cut(s)	1110
EcoO109I	RGGNCCY	1 cut(s)	675
EcoO65I	GGTNACC	1 cut(s)	1110
EcoRI	GAATTC	1 cut(s)	2156
EcoRII	CCWGG	1 cut(s)	846
EcoT38I	GRGCYC	3 cut(s)	553, 678, 2099
Esp3I	CGTCTC	1 cut(s)	1859
FaeI	CATG	7 cut(s)	353, 374, 431, 691, 1022, 1100, 1537
FalI	AAGNNNNNCTT	2 cut(s)	1633, 1665
FaqI	GGGAC	2 cut(s)	45, 1749
FatI	CATG	7 cut(s)	349, 370, 427, 687, 1018, 1096, 1533
FauI	CCCGC	2 cut(s)	2057, 2177
FauNDI	CATATG	1 cut(s)	402
FblI	GTMKAC	2 cut(s)	1518, 2042
Fnu4HI	GCNGC	4 cut(s)	779, 1316, 2179, 2182
FokI	GGATG	3 cut(s)	366, 1259, 1578
FriOI	GRGCYC	3 cut(s)	553, 678, 2099
Fsp4HI	GCNGC	4 cut(s)	779, 1316, 2179, 2182
FspAI	RTGCGCAY	1 cut(s)	686
FspBI	CTAG	1 cut(s)	843
FspI	TGCGCA	3 cut(s)	686, 1283, 1719
GlaI	GCGC	7 cut(s)	92, 102, 686, 1214, 1283, 1719, 2053
GluI	GCNGC	4 cut(s)	779, 1316, 2179, 2182
GsaI	CCCAGC	1 cut(s)	1701
GsuI	CTGGAG	1 cut(s)	1902
HaeIII	GGCC	4 cut(s)	474, 676, 1030, 1702
HapII	CCGG	3 cut(s)	88, 254, 1083
HgaI	GACGC	1 cut(s)	140
HhaI	GCGC	7 cut(s)	93, 103, 687, 1215, 1284, 1720, 2054
Hin1II	CATG	7 cut(s)	353, 374, 431, 691, 1022, 1100, 1537
Hin6I	GCGC	7 cut(s)	91, 101, 685, 1213, 1282, 1718, 2052
HinP1I	GCGC	7 cut(s)	91, 101, 685, 1213, 1282, 1718, 2052
HindIII	AAGCTT	1 cut(s)	922
HpaII	CCGG	3 cut(s)	88, 254, 1083
HphI	GGTGA	5 cut(s)	1104, 1142, 1535, 2028, 2152
Hpy166II	GTNNAC	7 cut(s)	53, 160, 467, 1150, 1170, 1519, 2043
Hpy8I	GTNNAC	7 cut(s)	53, 160, 467, 1150, 1170, 1519, 2043
Hpy99I	CGWCG	1 cut(s)	1094
HpyAV	CCTTC	3 cut(s)	1426, 1879, 1885
HpyCH4IV	ACGT	3 cut(s)	95, 469, 604
HpyCH4V	TGCA	8 cut(s)	218, 444, 765, 807, 1327, 1852, 1880, 2033
HpyF10VI	GCNNNNNNNGC	8 cut(s)	109, 650, 682, 778, 848, 1324, 1487, 2175
HpySE526I	ACGT	3 cut(s)	95, 469, 604
Hsp92II	CATG	7 cut(s)	353, 374, 431, 691, 1022, 1100, 1537
HspAI	GCGC	7 cut(s)	91, 101, 685, 1213, 1282, 1718, 2052
KpnI	GGTACC	1 cut(s)	1089
Kzo9I	GATC	5 cut(s)	518, 817, 1014, 1135, 1450
LmnI	GCTCC	3 cut(s)	556, 791, 2059
Lsp1109I	GCAGC	3 cut(s)	765, 1327, 2190
LweI	GCATC	8 cut(s)	344, 420, 586, 672, 1224, 1516, 1823, 2042
MaeI	CTAG	1 cut(s)	843
MaeII	ACGT	3 cut(s)	95, 469, 604
MaeIII	GTNAC	7 cut(s)	79, 338, 423, 741, 962, 1110, 1948
MalI	GATC	5 cut(s)	520, 819, 1016, 1137, 1452
MboI	GATC	5 cut(s)	518, 817, 1014, 1135, 1450
MfeI	CAATTG	1 cut(s)	1119
MflI	RGATCY	3 cut(s)	518, 817, 1450
MhlI	GDGCHC	4 cut(s)	553, 678, 1483, 2099
MluCI	AATT	9 cut(s)	29, 184, 626, 871, 944, 1119, 1257, 1807, 2156
MlyI	GAGTC	4 cut(s)	8, 239, 245, 1198
MmeI	TCCRAC	2 cut(s)	707, 1773
MroXI	GAANNNNTTC	2 cut(s)	1050, 1146
MseI	TTAA	5 cut(s)	458, 581, 624, 1260, 1353
MslI	CAYNNNNRTG	4 cut(s)	375, 1245, 1476, 2204
MspA1I	CMGCKG	1 cut(s)	2181
MspI	CCGG	3 cut(s)	88, 254, 1083
MspR9I	CCNGG	3 cut(s)	88, 255, 848
MunI	CAATTG	1 cut(s)	1119
Mva1269I	GAATGC	1 cut(s)	765
MvaI	CCWGG	1 cut(s)	848
MvnI	CGCG	1 cut(s)	2052
MwoI	GCNNNNNNNGC	8 cut(s)	109, 650, 682, 778, 848, 1324, 1487, 2175
NciI	CCSGG	2 cut(s)	88, 255
NdeI	CATATG	1 cut(s)	402
NdeII	GATC	5 cut(s)	518, 817, 1014, 1135, 1450
NheI	GCTAGC	1 cut(s)	842
NlaIII	CATG	7 cut(s)	353, 374, 431, 691, 1022, 1100, 1537
NlaIV	GGNNCC	6 cut(s)	439, 552, 676, 677, 819, 1087
NmeAIII	GCCGAG	1 cut(s)	1462
NmuCI	GTSAC	5 cut(s)	338, 423, 741, 962, 1110
NsbI	TGCGCA	3 cut(s)	686, 1283, 1719
NspI	RCATGY	2 cut(s)	374, 1100
NspV	TTCGAA	1 cut(s)	260
OliI	CACNNNNGTG	1 cut(s)	2204
PaqCI	CACCTGC	1 cut(s)	1723
PcsI	WCGNNNNNNNCGW	2 cut(s)	61, 1663
PctI	GAATGC	1 cut(s)	765
PdmI	GAANNNNTTC	2 cut(s)	1050, 1146
PfeI	GAWTC	9 cut(s)	286, 631, 1051, 1063, 1189, 1363, 1623, 1901, 2147
PkrI	GCNGC	4 cut(s)	780, 1317, 2180, 2183
PleI	GAGTC	4 cut(s)	8, 238, 244, 1198
PpsI	GAGTC	4 cut(s)	8, 238, 244, 1198
Ppu21I	YACGTR	2 cut(s)	470, 605
PshBI	ATTAAT	2 cut(s)	1260, 1353
PsiI	TTATAA	1 cut(s)	869
Psp6I	CCWGG	1 cut(s)	846
PspEI	GGTNACC	1 cut(s)	1110
PspFI	CCCAGC	1 cut(s)	1697
PspGI	CCWGG	1 cut(s)	846
PspN4I	GGNNCC	6 cut(s)	439, 552, 676, 677, 819, 1087
PspOMI	GGGCCC	1 cut(s)	674
PspPI	GGNCC	6 cut(s)	50, 473, 482, 674, 675, 1700
PstI	CTGCAG	2 cut(s)	446, 1854
PstNI	CAGNNNCTG	1 cut(s)	644
PsuI	RGATCY	3 cut(s)	518, 817, 1450
RsaI	GTAC	4 cut(s)	399, 468, 1087, 1759
RsaNI	GTAC	4 cut(s)	398, 467, 1086, 1758
RseI	CAYNNNNRTG	4 cut(s)	375, 1245, 1476, 2204
SaqAI	TTAA	5 cut(s)	458, 581, 624, 1260, 1353
SatI	GCNGC	4 cut(s)	779, 1316, 2179, 2182
Sau3AI	GATC	5 cut(s)	518, 817, 1014, 1135, 1450
Sau96I	GGNCC	6 cut(s)	50, 473, 482, 674, 675, 1700
SchI	GAGTC	4 cut(s)	8, 239, 245, 1198
ScrFI	CCNGG	3 cut(s)	88, 255, 848
SduI	GDGCHC	4 cut(s)	553, 678, 1483, 2099
SfaNI	GCATC	8 cut(s)	344, 420, 586, 672, 1224, 1516, 1823, 2042
SfcI	CTRYAG	6 cut(s)	381, 442, 573, 822, 1850, 2043
SfuI	TTCGAA	1 cut(s)	260
SinI	GGWCC	2 cut(s)	50, 482
SmiMI	CAYNNNNRTG	4 cut(s)	375, 1245, 1476, 2204
SmlI	CTYRAG	3 cut(s)	300, 1305, 1753
SmoI	CTYRAG	3 cut(s)	300, 1305, 1753
Sse9I	AATT	9 cut(s)	29, 184, 626, 871, 944, 1119, 1257, 1807, 2156
SsiI	CCGC	7 cut(s)	953, 1501, 2050, 2104, 2163, 2181, 2184
SspI	AATATT	2 cut(s)	1264, 1351
SspMI	CTAG	1 cut(s)	843
StyD4I	CCNGG	3 cut(s)	86, 253, 846
TaiI	ACGT	3 cut(s)	98, 472, 607
TaqI	TCGA	3 cut(s)	260, 394, 1092
TasI	AATT	9 cut(s)	29, 184, 626, 871, 944, 1119, 1257, 1807, 2156
TatI	WGTACW	2 cut(s)	397, 1757
TauI	GCSGC	1 cut(s)	2184
TfiI	GAWTC	9 cut(s)	286, 631, 1051, 1063, 1189, 1363, 1623, 1901, 2147
Tru1I	TTAA	5 cut(s)	458, 581, 624, 1260, 1353
Tru9I	TTAA	5 cut(s)	458, 581, 624, 1260, 1353
TscAI	CASTG	5 cut(s)	225, 469, 900, 1012, 1177
TseFI	GTSAC	5 cut(s)	338, 423, 741, 962, 1110
TseI	GCWGC	3 cut(s)	778, 1315, 2178
Tsp45I	GTSAC	5 cut(s)	338, 423, 741, 962, 1110
TspDTI	ATGAA	6 cut(s)	38, 338, 342, 1059, 1089, 1944
TspGWI	ACGGA	2 cut(s)	727, 1994
TspRI	CASTG	5 cut(s)	225, 469, 900, 1012, 1177
VpaK11BI	GGWCC	2 cut(s)	50, 482
VspI	ATTAAT	2 cut(s)	1260, 1353
XapI	RAATTY	4 cut(s)	871, 944, 1807, 2156
XceI	RCATGY	2 cut(s)	374, 1100
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1240
XmiI	GTMKAC	2 cut(s)	1518, 2042
XmnI	GAANNNNTTC	2 cut(s)	1050, 1146
XspI	CTAG	1 cut(s)	843

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.