Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0003097

Physical Location & Seq

Genomic Coordinates Forward (+)

48450782 .. 48464232

Gene Structure 13451 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG424700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGAATGGATCTGAGGAGCCCGAATCGAAGTATGGATCTGGTTATGGGCGAAAACTGGAGTATAAGGCACTGGAATCGGAGTATGGACGGAAAACCCGAGTACGAAGTGCCAGTCGGGTCGGGCTATGGCCAGAAGCCAAGTTACTGAGAGGATGGGAGTGCTGGATAAGGCCACGAGCCGCCGAGGAAGCTATAGGAGGCCCGGCTATGGAAAAGGGACCCGAGTTGAGAATTTGGGTTTATGGTGATGAACTGCCAAGGAAGCTTGGGTATGGAAGGTCTGGTCGTTATGGGGGAAAGGAGGAGAGTGAGAACCCACCCGGTTACCTCCCGGCGCACGTTAGCGCAACGCCAGCCATTTCGGGCTTCTCCACACCTCTTTCTCACACACGCCAGCGTCCATTTCACGAAGCCTTCAATCTTCAGAAAAATTATCATCGTCTTCTCTCCTTTGGAGGTGTTGCACTGATTTGGGAGTCTGAGTCTTGGAGAACCGTGCCGGGTTCGAAAGCATTGTGGAGAGAGTTTGAGAATCAAGAAATGCCTCAAGCTGGTTCTATGAGGTTGTTGTATGAATGGAGAAGTCACAGTATTCATGGATGCCATAAACAGCCACATGCTTGTGGTGTAGGAAAGGGTCGAGTACATATGGTTAGCATCAATCTTCCGTCGCATGGCTTGGGTGCCTGCAGTCTCCATCTTTTAAGCAGCGTACGTGGCCCAATAGGTCCAGTATCTTCCAGATGTAAAGTTTTCTTGTGTAGATCTGTTTTTATACCAAGTGGAGGAACTGGGGCTCCTGCTCTGAAATCTACTGCTGTAGTTTTAACAAGGTCTTATGATGCTTTACGTGGAAGCCCTGTTTTCCTTAAATTGATTCAGGCAGTTGCCATCATTGCTTTTGCTGTTTGGGGTCTTGGGCCCCTGATGCGCATGGGGAGGATTATATTTTTTCAACGGACTGATAGTAGTTGGAAGAAAAGTAGGTCACACTATGCTATGACCTCTTGCATTCAGCCTTTGCTGCTTTGGGGTGGAGCAATGCTTGTATGCAGAACATTGGATCCTGTAGTTCTACCATCAGAAGCTACCCAGGCTGTCAAGCAAAGACTTATAAATTTTTTACAATCATTATCAACAGTGCTGGCGTTTGCCTACTGTTTGTCAAGCTTGAACCAACAAGCACAGAAATTCTTTGCGGAGACAAGTGACCCAAGTGATACCAGAAATATGGGCTTCAATTTTGCTGGCAAAGCTGTTTATACTGCAGTTTGGGTTGCTGCAGTGTCATTGTTTATGGAGTTGCTGGGATTCTCGACCCAGAAATGGCTAACAGCTGGGGGTCTCGGCACAGTATTGCTAACCCTTGCTGGTCGTGAGATATTCACAAACTTCCTTTCCAGTGTAATGATCCATGCCACACGGCCATTTGTTGTCAATGAATGGATTCAGACTAAGATTCAAGGCTATGAAGTTTCTGGTACCGTCGAGCATGTGGGGTGGTGGTCACCAACAATTATAAGAGGCGATGATCGTGAAGCAGTTCACATTCCAAATCACAAGTTCACTGTGAATGTTGTGAGGAATCTTAGCCAGAAGACTCATTGGCGCATCAAGAACCACATAGCCATTAGTCATTTGGATGTCAATAAAATTAATATTATTGTAGCTGATATGCGCAAGGTTTTAGCCAAAAATCCTCAAGTAGAGCAGCAAAGGTTGCATAGAAGAGTTTTTCTGGATGATATTAATCCAGAGAATCAGGCTCTTATGATTTTGGTATCTTGCTTTGTGAAAACATCTCACATTGAGGAGTATCTCTGCGTGAAGGAGGCAATACTCTTGGATCTTCTCAGAGTTGTCAGTCATCATCGTGCTCGGCTTGCCACACCCCTTCGCACAGTTCAGAAAGTATACGGTGATGCTGACATGGAAAATGTGCCATTTGCTGACACCATTTTTGGTAGTTCTACGGCACGTACTAATCGTCCATATCTCCTCATTGAGCCATCTTACAGAATCAGTAGTGATGACAAAAGTAAGGCTTCTAATCGCTCAGCACGTACAAATGGAGACAAGGAAGAACCTAAAGCTGGGCCAACACTAACACCTGCGCAAACTGAAGATAAGGCAACTTCAACTTCCAACTCAAGTACAAGTCCCAAGACTTCGGAAATGCCAACATCTGAACCCCAAACACAGAATTTGGCATCTGATGGTTCAGCCCCAGACTCTTCTGAAATACTGCAGTCCAAGAATGAGATGATAAGATTTGCAGGGAAGGAGAAGGGAGTTGATTCCAAAAATGTATCTCCAGAAAAGGCGACATCTAAGAAGTCTTCTGTTACTTCATCTGAGATGGGTGGTGGAAAGACGGATATTCCTTTGACAACTTTACAGGCAAAACAAGATGGAGAGAAACCTGTTGCATCACCGTCTACAGCTCGCGCTCCCTTAGAAGAGAATATCATTCTCGGTGTTGCCTTAGAGGGCTCAAAGCGGACACTTCCCATTGAGGAAGATATGGCTCCATCTCTTATAGAATCAAAAGAATTCACCGCCAGCCGTAATGGCAGCGGCGGGTCTCCTCCTGGCACAGATGTGAAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

870

Amino Acids

95.81

Weight (kDa)

9.34

Isoelectric Point (pI)

49.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	331 - 459	5e-67	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	461 - 531	7e-12	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	536 - 622	2e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58200 AT1G58200
fragaria_vesca	FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370
malus_domestica	MD08G1197800.v1.1
prunus_persica	Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1
pyrus_communis	pycom15g34530
rosa_chinensis	RchiOBHm_Chr7g0236721
rosa_laevigata	RLG00000001066
rosa_multiflora	Rmu_co8258783.1_g000001 Rmu_sc0003720.1_g000014 Rmu_sc0011298.1_g000003
rosa_roxburghii	Rroxscaffold_3G00224920
rosa_rugosa	Rorug07G0298400 Rorug07G0298500 Rorug07G0298600
rosa_samantha	Rh7AG454000 Rh7BG424700 Rh7CG472300 Rh7DG441300
rosa_wichuraiana	Rw7G037640 Rw7G037670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1115, 1520
AarI	CACCTGC	1 cut(s)	2119
Acc16I	TGCGCA	3 cut(s)	932, 1679, 2115
Acc36I	ACCTGC	1 cut(s)	2119
Acc65I	GGTACC	1 cut(s)	1481
AccB1I	GGYRCC	2 cut(s)	683, 1481
AccI	GTMKAC	2 cut(s)	1914, 2438
AccII	CGCG	1 cut(s)	2448
AciI	CCGC	6 cut(s)	180, 1199, 2500, 2559, 2577, 2580
AclWI	GGATC	6 cut(s)	16, 43, 1058, 1071, 1405, 1854
AcoI	YGGCCR	2 cut(s)	128, 1424
AcsI	RAATTY	5 cut(s)	231, 1117, 1190, 2203, 2552
AcuI	CTGAAG	2 cut(s)	407, 2142
AfaI	GTAC	7 cut(s)	102, 645, 714, 1483, 1981, 2065, 2155
AfiI	CCNNNNNNNGG	6 cut(s)	208, 551, 674, 785, 1326, 2093
AgsI	TTSAA	6 cut(s)	418, 956, 1174, 1240, 1463, 2139
AjnI	CCWGG	2 cut(s)	1092, 2590
AleI	CACNNNNGTG	1 cut(s)	2600
AloI	GAACNNNNNNTCC	4 cut(s)	781, 813, 1048, 1080
Alw21I	GWGCWC	1 cut(s)	1879
Alw26I	GTCTC	5 cut(s)	698, 1196, 1349, 2067, 2589
AlwI	GGATC	6 cut(s)	16, 43, 1058, 1071, 1405, 1854
Ama87I	CYCGRG	2 cut(s)	96, 221
AoxI	GGCC	7 cut(s)	128, 170, 199, 718, 920, 1424, 2096
ApaI	GGGCCC	1 cut(s)	924
ApeKI	GCWGC	5 cut(s)	708, 1024, 1280, 1711, 2574
ApoI	RAATTY	5 cut(s)	231, 1117, 1190, 2203, 2552
ArsI	GACNNNNNNTTYG	4 cut(s)	97, 129, 1943, 1975
AseI	ATTAAT	2 cut(s)	1656, 1749
Asp700I	GAANNNNTTC	2 cut(s)	1446, 1542
Asp718I	GGTACC	1 cut(s)	1481
AspLEI	GCGC	7 cut(s)	337, 347, 933, 1611, 1680, 2116, 2450
AspS9I	GGNCC	7 cut(s)	200, 218, 719, 728, 920, 921, 2096
AsuC2I	CCSGG	4 cut(s)	203, 321, 332, 501
AsuHPI	GGTGA	5 cut(s)	257, 1500, 1931, 2424, 2548
AsuII	TTCGAA	1 cut(s)	506
AvaI	CYCGRG	2 cut(s)	96, 221
AvaII	GGWCC	2 cut(s)	218, 728
BaeGI	GKGCMC	1 cut(s)	924
BalI	TGGCCA	1 cut(s)	130
BamHI	GGATCC	1 cut(s)	1063
BanI	GGYRCC	2 cut(s)	683, 1481
BanII	GRGCYC	4 cut(s)	21, 799, 924, 2495
BarI	GAAGNNNNNNTAC	2 cut(s)	126, 158
BauI	CACGAG	1 cut(s)	174
BbsI	GAAGAC	3 cut(s)	434, 1604, 2331
Bbv12I	GWGCWC	1 cut(s)	1879
BbvI	GCAGC	5 cut(s)	720, 1011, 1267, 1723, 2586
BccI	CCATC	9 cut(s)	147, 705, 899, 1087, 2017, 2210, 2353, 2405, 2539
BceAI	ACGGC	3 cut(s)	1439, 1989, 2550
BcgI	CGANNNNNNTGC	2 cut(s)	57, 91
BciT130I	CCWGG	2 cut(s)	1094, 2592
BcnI	CCSGG	4 cut(s)	203, 321, 332, 501
BcoDI	GTCTC	5 cut(s)	698, 1196, 1349, 2067, 2589
BfmI	CTRYAG	8 cut(s)	192, 688, 819, 1068, 1266, 1281, 2246, 2439
BfuAI	ACCTGC	1 cut(s)	2119
BglI	GCCNNNNNGGC	1 cut(s)	2571
BglII	AGATCT	1 cut(s)	764
BisI	GCNGC	7 cut(s)	180, 709, 1025, 1281, 1712, 2575, 2578
BlpI	GCTNAGC	1 cut(s)	2056
BlsI	GCNGC	7 cut(s)	181, 710, 1026, 1282, 1713, 2576, 2579
Bme1390I	CCNGG	6 cut(s)	203, 321, 332, 501, 1094, 2592
Bme18I	GGWCC	2 cut(s)	218, 728
BmeT110I	CYCGRG	2 cut(s)	96, 221
BmgT120I	GGNCC	7 cut(s)	200, 218, 719, 728, 920, 921, 2096
BmrFI	CCNGG	6 cut(s)	203, 321, 332, 501, 1094, 2592
BmrI	ACTGGG	1 cut(s)	801
BmsI	GCATC	8 cut(s)	590, 666, 832, 918, 1620, 1912, 2219, 2438
BmuI	ACTGGG	1 cut(s)	801
BpiI	GAAGAC	3 cut(s)	434, 1604, 2331
BpmI	CTGGAG	2 cut(s)	77, 2298
Bpu1102I	GCTNAGC	1 cut(s)	2056
Bpu14I	TTCGAA	1 cut(s)	506
BpuEI	CTTGAG	3 cut(s)	531, 1686, 2134
BpuMI	CCSGG	4 cut(s)	203, 321, 332, 501
BsaAI	YACGTR	4 cut(s)	716, 851, 1979, 2063
BsaBI	GATNNNNATC	1 cut(s)	1749
BsaI	GGTCTC	2 cut(s)	1349, 2589
BsaJI	CCNNGG	3 cut(s)	183, 257, 1092
BsaXI	ACNNNNNCTCC	4 cut(s)	467, 497, 781, 811
Bsc4I	CCNNNNNNNGG	6 cut(s)	208, 551, 674, 785, 1326, 2093
Bse1I	ACTGG	6 cut(s)	60, 75, 111, 731, 796, 1401
Bse3DI	GCAATG	2 cut(s)	894, 1047
Bse8I	GATNNNNATC	1 cut(s)	1749
BseBI	CCWGG	2 cut(s)	1094, 2592
BseDI	CCNNGG	3 cut(s)	183, 257, 1092
BseGI	GGATG	4 cut(s)	158, 605, 1648, 1747
BseJI	GATNNNNATC	1 cut(s)	1749
BseLI	CCNNNNNNNGG	6 cut(s)	208, 551, 674, 785, 1326, 2093
BseMI	GCAATG	2 cut(s)	894, 1047
BseMII	CTCAG	5 cut(s)	137, 471, 1867, 2070, 2346
BseNI	ACTGG	6 cut(s)	60, 75, 111, 731, 796, 1401
BseRI	GAGGAG	5 cut(s)	29, 317, 1826, 1988, 2577
BseSI	GKGCMC	1 cut(s)	924
BseXI	GCAGC	5 cut(s)	720, 1011, 1267, 1723, 2586
BseYI	CCCAGC	3 cut(s)	1306, 1337, 2093
Bsh1236I	CGCG	1 cut(s)	2448
BshFI	GGCC	7 cut(s)	130, 172, 201, 720, 922, 1426, 2098
BshNI	GGYRCC	2 cut(s)	683, 1481
BsiHKAI	GWGCWC	1 cut(s)	1879
BsiHKCI	CYCGRG	2 cut(s)	96, 221
BsiSI	CCGG	4 cut(s)	203, 321, 332, 500
BsiWI	CGTACG	1 cut(s)	712
BslFI	GGGAC	2 cut(s)	231, 2145
BslI	CCNNNNNNNGG	6 cut(s)	208, 551, 674, 785, 1326, 2093
BsmAI	GTCTC	5 cut(s)	698, 1196, 1349, 2067, 2589
BsmFI	GGGAC	2 cut(s)	231, 2145
BsmI	GAATGC	1 cut(s)	1011
BsnI	GGCC	7 cut(s)	130, 172, 201, 720, 922, 1426, 2098
Bso31I	GGTCTC	2 cut(s)	1349, 2589
BsoBI	CYCGRG	2 cut(s)	96, 221
Bsp119I	TTCGAA	1 cut(s)	506
Bsp120I	GGGCCC	1 cut(s)	920
Bsp1286I	GDGCHC	5 cut(s)	21, 799, 924, 1879, 2495
Bsp143I	GATC	7 cut(s)	8, 35, 764, 1063, 1410, 1531, 1846
Bsp1720I	GCTNAGC	1 cut(s)	2056
BspACI	CCGC	6 cut(s)	180, 1199, 2500, 2559, 2577, 2580
BspANI	GGCC	7 cut(s)	130, 172, 201, 720, 922, 1426, 2098
BspCNI	CTCAG	6 cut(s)	4, 138, 472, 1866, 2069, 2347
BspFNI	CGCG	1 cut(s)	2448
BspMAI	CTGCAG	4 cut(s)	692, 1270, 1285, 2250
BspMI	ACCTGC	1 cut(s)	2119
BspPI	GGATC	6 cut(s)	16, 43, 1058, 1071, 1405, 1854
BspT104I	TTCGAA	1 cut(s)	506
BspT107I	GGYRCC	2 cut(s)	683, 1481
BspTNI	GGTCTC	2 cut(s)	1349, 2589
BsrDI	GCAATG	2 cut(s)	894, 1047
BsrI	ACTGG	6 cut(s)	60, 75, 111, 731, 796, 1401
BssECI	CCNNGG	3 cut(s)	183, 257, 1092
BssMI	GATC	7 cut(s)	8, 35, 764, 1063, 1410, 1531, 1846
BssNAI	GTATAC	1 cut(s)	1915
BssSI	CACGAG	1 cut(s)	174
BssT1I	CCWWGG	1 cut(s)	257
Bst1107I	GTATAC	1 cut(s)	1915
Bst2BI	CACGAG	1 cut(s)	174
Bst2UI	CCWGG	2 cut(s)	1094, 2592
Bst6I	CTCTTC	3 cut(s)	1723, 2239, 2454
BstAPI	GCANNNNNTGC	1 cut(s)	1720
BstBAI	YACGTR	4 cut(s)	716, 851, 1979, 2063
BstBI	TTCGAA	1 cut(s)	506
BstC8I	GCNNGC	8 cut(s)	354, 395, 688, 1146, 1249, 1884, 2446, 2563
BstEII	GGTNACC	2 cut(s)	323, 1506
BstF5I	GGATG	4 cut(s)	158, 605, 1648, 1747
BstFNI	CGCG	1 cut(s)	2448
BstHHI	GCGC	7 cut(s)	337, 347, 933, 1611, 1680, 2116, 2450
BstKTI	GATC	7 cut(s)	11, 38, 767, 1066, 1413, 1534, 1849
BstMAI	GTCTC	5 cut(s)	698, 1196, 1349, 2067, 2589
BstMBI	GATC	7 cut(s)	8, 35, 764, 1063, 1410, 1531, 1846
BstNI	CCWGG	2 cut(s)	1094, 2592
BstNSI	RCATGY	2 cut(s)	620, 1496
BstPI	GGTNACC	2 cut(s)	323, 1506
BstSCI	CCNGG	6 cut(s)	201, 319, 330, 499, 1092, 2590
BstSFI	CTRYAG	8 cut(s)	192, 688, 819, 1068, 1266, 1281, 2246, 2439
BstSLI	GKGCMC	1 cut(s)	924
BstUI	CGCG	1 cut(s)	2448
BstV1I	GCAGC	5 cut(s)	720, 1011, 1267, 1723, 2586
BstV2I	GAAGAC	3 cut(s)	434, 1604, 2331
BstX2I	RGATCY	5 cut(s)	8, 35, 764, 1063, 1846
BstXI	CCANNNNNNTGG	1 cut(s)	1231
BstYI	RGATCY	5 cut(s)	8, 35, 764, 1063, 1846
BstZ17I	GTATAC	1 cut(s)	1915
BsuRI	GGCC	7 cut(s)	130, 172, 201, 720, 922, 1426, 2098
BtgZI	GCGATG	1 cut(s)	1542
BtsCI	GGATG	4 cut(s)	158, 605, 1648, 1747
BtsI	GCAGTG	1 cut(s)	1290
BtsIMutI	CAGTG	6 cut(s)	68, 464, 1146, 1290, 1408, 1566
BveI	ACCTGC	1 cut(s)	2119
Cac8I	GCNNGC	8 cut(s)	354, 395, 688, 1146, 1249, 1884, 2446, 2563
CfoI	GCGC	7 cut(s)	337, 347, 933, 1611, 1680, 2116, 2450
Cfr13I	GGNCC	7 cut(s)	200, 218, 719, 728, 920, 921, 2096
CseI	GACGC	1 cut(s)	386
Csp6I	GTAC	7 cut(s)	101, 644, 713, 1482, 1980, 2064, 2154
CviAII	CATG	7 cut(s)	596, 617, 674, 934, 1415, 1493, 1930
CviQI	GTAC	7 cut(s)	101, 644, 713, 1482, 1980, 2064, 2154
DpnI	GATC	7 cut(s)	10, 37, 766, 1065, 1412, 1533, 1848
DpnII	GATC	7 cut(s)	8, 35, 764, 1063, 1410, 1531, 1846
EaeI	YGGCCR	2 cut(s)	128, 1424
Eam1104I	CTCTTC	3 cut(s)	1723, 2239, 2454
EarI	CTCTTC	3 cut(s)	1723, 2239, 2454
Eco130I	CCWWGG	1 cut(s)	257
Eco24I	GRGCYC	4 cut(s)	21, 799, 924, 2495
Eco31I	GGTCTC	2 cut(s)	1349, 2589
Eco47I	GGWCC	2 cut(s)	218, 728
Eco57I	CTGAAG	2 cut(s)	407, 2142
Eco88I	CYCGRG	2 cut(s)	96, 221
Eco91I	GGTNACC	2 cut(s)	323, 1506
EcoO109I	RGGNCCY	2 cut(s)	218, 921
EcoO65I	GGTNACC	2 cut(s)	323, 1506
EcoRI	GAATTC	1 cut(s)	2552
EcoRII	CCWGG	2 cut(s)	1092, 2590
EcoT14I	CCWWGG	1 cut(s)	257
EcoT38I	GRGCYC	4 cut(s)	21, 799, 924, 2495
ErhI	CCWWGG	1 cut(s)	257
FaeI	CATG	7 cut(s)	599, 620, 677, 937, 1418, 1496, 1933
FalI	AAGNNNNNCTT	2 cut(s)	2029, 2061
FaqI	GGGAC	2 cut(s)	231, 2145
FatI	CATG	7 cut(s)	595, 616, 673, 933, 1414, 1492, 1929
FauI	CCCGC	1 cut(s)	2573
FauNDI	CATATG	1 cut(s)	648
FblI	GTMKAC	2 cut(s)	1914, 2438
Fnu4HI	GCNGC	7 cut(s)	180, 709, 1025, 1281, 1712, 2575, 2578
FokI	GGATG	4 cut(s)	165, 612, 1655, 1754
FriOI	GRGCYC	4 cut(s)	21, 799, 924, 2495
Fsp4HI	GCNGC	7 cut(s)	180, 709, 1025, 1281, 1712, 2575, 2578
FspAI	RTGCGCAY	1 cut(s)	932
FspI	TGCGCA	3 cut(s)	932, 1679, 2115
GlaI	GCGC	7 cut(s)	336, 346, 932, 1610, 1679, 2115, 2449
GluI	GCNGC	7 cut(s)	180, 709, 1025, 1281, 1712, 2575, 2578
GsaI	CCCAGC	3 cut(s)	1310, 1341, 2097
GsuI	CTGGAG	2 cut(s)	77, 2298
HaeIII	GGCC	7 cut(s)	130, 172, 201, 720, 922, 1426, 2098
HapII	CCGG	4 cut(s)	203, 321, 332, 500
HgaI	GACGC	1 cut(s)	386
HhaI	GCGC	7 cut(s)	337, 347, 933, 1611, 1680, 2116, 2450
Hin1II	CATG	7 cut(s)	599, 620, 677, 937, 1418, 1496, 1933
Hin6I	GCGC	7 cut(s)	335, 345, 931, 1609, 1678, 2114, 2448
HinP1I	GCGC	7 cut(s)	335, 345, 931, 1609, 1678, 2114, 2448
HindIII	AAGCTT	2 cut(s)	263, 1168
HpaII	CCGG	4 cut(s)	203, 321, 332, 500
HphI	GGTGA	5 cut(s)	257, 1500, 1931, 2424, 2548
Hpy166II	GTNNAC	4 cut(s)	1546, 1566, 1915, 2439
Hpy8I	GTNNAC	4 cut(s)	1546, 1566, 1915, 2439
Hpy99I	CGWCG	2 cut(s)	673, 1490
HpyAV	CCTTC	6 cut(s)	270, 424, 1822, 1904, 2275, 2281
HpyCH4IV	ACGT	5 cut(s)	339, 715, 850, 1978, 2062
HpySE526I	ACGT	5 cut(s)	339, 715, 850, 1978, 2062
Hsp92II	CATG	7 cut(s)	599, 620, 677, 937, 1418, 1496, 1933
HspAI	GCGC	7 cut(s)	335, 345, 931, 1609, 1678, 2114, 2448
KflI	GGGWCCC	1 cut(s)	218
KpnI	GGTACC	1 cut(s)	1485
Kzo9I	GATC	7 cut(s)	8, 35, 764, 1063, 1410, 1531, 1846
LmnI	GCTCC	5 cut(s)	16, 802, 1037, 2455, 2533
Lsp1109I	GCAGC	5 cut(s)	720, 1011, 1267, 1723, 2586
LweI	GCATC	8 cut(s)	590, 666, 832, 918, 1620, 1912, 2219, 2438
MaeII	ACGT	5 cut(s)	339, 715, 850, 1978, 2062
MaeIII	GTNAC	7 cut(s)	141, 323, 584, 987, 1208, 1506, 2344
MalI	GATC	7 cut(s)	10, 37, 766, 1065, 1412, 1533, 1848
MboI	GATC	7 cut(s)	8, 35, 764, 1063, 1410, 1531, 1846
MflI	RGATCY	5 cut(s)	8, 35, 764, 1063, 1846
MhlI	GDGCHC	5 cut(s)	21, 799, 924, 1879, 2495
MlsI	TGGCCA	1 cut(s)	130
MluNI	TGGCCA	1 cut(s)	130
MlyI	GAGTC	4 cut(s)	485, 491, 1594, 2225
MmeI	TCCRAC	2 cut(s)	953, 2169
Mox20I	TGGCCA	1 cut(s)	130
MroXI	GAANNNNTTC	2 cut(s)	1446, 1542
MscI	TGGCCA	1 cut(s)	130
MseI	TTAA	5 cut(s)	704, 827, 870, 1656, 1749
MslI	CAYNNNNRTG	4 cut(s)	621, 1641, 1872, 2600
Msp20I	TGGCCA	1 cut(s)	130
MspA1I	CMGCKG	2 cut(s)	1337, 2577
MspI	CCGG	4 cut(s)	203, 321, 332, 500
MspR9I	CCNGG	6 cut(s)	203, 321, 332, 501, 1094, 2592
Mva1269I	GAATGC	1 cut(s)	1011
MvaI	CCWGG	2 cut(s)	1094, 2592
MvnI	CGCG	1 cut(s)	2448
NciI	CCSGG	4 cut(s)	203, 321, 332, 501
NdeI	CATATG	1 cut(s)	648
NdeII	GATC	7 cut(s)	8, 35, 764, 1063, 1410, 1531, 1846
NlaIII	CATG	7 cut(s)	599, 620, 677, 937, 1418, 1496, 1933
NmeAIII	GCCGAG	3 cut(s)	208, 1326, 1858
NmuCI	GTSAC	4 cut(s)	584, 987, 1208, 1506
NsbI	TGCGCA	3 cut(s)	932, 1679, 2115
NspI	RCATGY	2 cut(s)	620, 1496
NspV	TTCGAA	1 cut(s)	506
OliI	CACNNNNGTG	1 cut(s)	2600
PaqCI	CACCTGC	1 cut(s)	2119
PcsI	WCGNNNNNNNCGW	3 cut(s)	94, 1984, 2059
PctI	GAATGC	1 cut(s)	1011
PdmI	GAANNNNTTC	2 cut(s)	1446, 1542
Pfl23II	CGTACG	1 cut(s)	712
PkrI	GCNGC	7 cut(s)	181, 710, 1026, 1282, 1713, 2576, 2579
PleI	GAGTC	4 cut(s)	484, 490, 1594, 2225
PpsI	GAGTC	4 cut(s)	484, 490, 1594, 2225
Ppu21I	YACGTR	4 cut(s)	716, 851, 1979, 2063
PpuMI	RGGWCCY	1 cut(s)	218
PshBI	ATTAAT	2 cut(s)	1656, 1749
PsiI	TTATAA	2 cut(s)	1115, 1520
Psp5II	RGGWCCY	1 cut(s)	218
Psp6I	CCWGG	2 cut(s)	1092, 2590
PspEI	GGTNACC	2 cut(s)	323, 1506
PspFI	CCCAGC	3 cut(s)	1306, 1337, 2093
PspGI	CCWGG	2 cut(s)	1092, 2590
PspLI	CGTACG	1 cut(s)	712
PspOMI	GGGCCC	1 cut(s)	920
PspPI	GGNCC	7 cut(s)	200, 218, 719, 728, 920, 921, 2096
PspPPI	RGGWCCY	1 cut(s)	218
PstI	CTGCAG	4 cut(s)	692, 1270, 1285, 2250
PsuI	RGATCY	5 cut(s)	8, 35, 764, 1063, 1846
PvuII	CAGCTG	1 cut(s)	1337
RsaI	GTAC	7 cut(s)	102, 645, 714, 1483, 1981, 2065, 2155
RsaNI	GTAC	7 cut(s)	101, 644, 713, 1482, 1980, 2064, 2154
RseI	CAYNNNNRTG	4 cut(s)	621, 1641, 1872, 2600
SaqAI	TTAA	5 cut(s)	704, 827, 870, 1656, 1749
SatI	GCNGC	7 cut(s)	180, 709, 1025, 1281, 1712, 2575, 2578
Sau3AI	GATC	7 cut(s)	8, 35, 764, 1063, 1410, 1531, 1846
Sau96I	GGNCC	7 cut(s)	200, 218, 719, 728, 920, 921, 2096
SchI	GAGTC	4 cut(s)	485, 491, 1594, 2225
ScrFI	CCNGG	6 cut(s)	203, 321, 332, 501, 1094, 2592
SduI	GDGCHC	5 cut(s)	21, 799, 924, 1879, 2495
SfaNI	GCATC	8 cut(s)	590, 666, 832, 918, 1620, 1912, 2219, 2438
SfcI	CTRYAG	8 cut(s)	192, 688, 819, 1068, 1266, 1281, 2246, 2439
SfuI	TTCGAA	1 cut(s)	506
SinI	GGWCC	2 cut(s)	218, 728
SmiMI	CAYNNNNRTG	4 cut(s)	621, 1641, 1872, 2600
SmlI	CTYRAG	3 cut(s)	546, 1701, 2149
SmoI	CTYRAG	3 cut(s)	546, 1701, 2149
SsiI	CCGC	6 cut(s)	180, 1199, 2500, 2559, 2577, 2580
SspI	AATATT	1 cut(s)	1660
StyD4I	CCNGG	6 cut(s)	201, 319, 330, 499, 1092, 2590
StyI	CCWWGG	1 cut(s)	257
TaiI	ACGT	5 cut(s)	342, 718, 853, 1981, 2065
TaqI	TCGA	5 cut(s)	26, 506, 640, 1316, 1488
TatI	WGTACW	2 cut(s)	643, 2153
TauI	GCSGC	2 cut(s)	182, 2580
Tru1I	TTAA	5 cut(s)	704, 827, 870, 1656, 1749
Tru9I	TTAA	5 cut(s)	704, 827, 870, 1656, 1749
TscAI	CASTG	6 cut(s)	75, 471, 1146, 1290, 1408, 1573
TseFI	GTSAC	4 cut(s)	584, 987, 1208, 1506
TseI	GCWGC	5 cut(s)	708, 1024, 1280, 1711, 2574
Tsp45I	GTSAC	4 cut(s)	584, 987, 1208, 1506
TspDTI	ATGAA	7 cut(s)	17, 264, 584, 588, 1455, 1485, 2340
TspGWI	ACGGA	4 cut(s)	103, 657, 973, 2390
TspRI	CASTG	6 cut(s)	75, 471, 1146, 1290, 1408, 1573
VpaK11BI	GGWCC	2 cut(s)	218, 728
VspI	ATTAAT	2 cut(s)	1656, 1749
XapI	RAATTY	5 cut(s)	231, 1117, 1190, 2203, 2552
XceI	RCATGY	2 cut(s)	620, 1496
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1636
XmiI	GTMKAC	2 cut(s)	1914, 2438
XmnI	GAANNNNTTC	2 cut(s)	1446, 1542

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.