Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003097

Physical Location & Seq

Genomic Coordinates Forward (+)

61401166 .. 61406935

Gene Structure 5770 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ21214

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGCCTCAAGCTGGTTCTATGAGGTTGTTGTATGAATGGAGAAGTCACAGTATTCATGGATGCCATAAACAGCCACATGCTTGTGGTGTAGGAAAGGGTCGAGTACATATGGTTAGCATCAATCTACCGTCACATGGCTTGGGTGCCTGCAGTCTCCATCTTTTAAGCAGTGTACGTGGCCCAATAGGTCCAGTATCTTCCAGATGTAAAGTTTTCTTGTGTAGATCTGTTTTTATACCAAGTGGAGGAACTGGGGCTCCTGCTCTGAAATCTACTGCTGTAGTTTTAACAAGGTCTTATGATGCTTTACGTGGAAGCCCTGTTTTCCTTAAATTGATTCAGGCAGTTGCTGTCATTGCTTTTGCTGTTTGGGGTCTTGGGCCCCTGATGCGCATGGGGAGGATTATATTTTTTCAACGGACTGATAGTAGTTGGAAGAAAAGTAGGTCACACTATGCTATGACCTCTTGCATTCAGCCTTTGCTGCTTTGGGGTGGAGCAATGCTTGTATGCAGGGCATTGGATCCTGTAGTTCTACCATCAGAAGCTAGCCAGGCTGTCAAGCAAAGACTTGTAAATTTTTCACAATCATTATCAACAGTGCTGGCGTTTGCCTACTGTTTGTCAAGCTTGAACCAACAAGCACAGAAATTCTTTGCGGAGACAAGTGACCCAAGTGATACCAGAAATATGGGATTCAATTTTGCTGGCAAAGCTGTTTATACTGCAGTTTGGGTTGCTGCAGTGTCATTGTTTATGGAGTTGCTGGGATTCTCGACCCAGAAATGGCTAACAGCTGGGGGTCTCGGCACAGTATTGCTAACCCTTGCTGGTCGTGAGATATTCACAAACTTCCTTTCCAGTGTAATGATCCATGCCACACGGCCATTTGTTGTCAATGAATGGATTCAGACTAAGATTGAAGGCTATGAGGTTTCTGGTACTGTCGAGCATGTGGGGTGGTGGTCACCAACAATTATAAGAGGCGATGATCGTGAAGCAGTTCACATTCCAAATCACAAGTTCACTGTGAATGTTGTGAGGAATCTTAGCCAGAAGACTCATTGGCGCATCAAGAACCACATAGCCATTAGTCATTTGGATGTCAATAAAATTAATATTATTGTAGCTGATATGCGCAAAGTTTTAGCCAAAAATCCTCAAGTAGAGCAGCAAAGGTTGCATAGAAGAGTTTTTCTGGATAATATTAATCCAGAGAATCAGGCTCTTATGATTTTGGTATCTTGCTTTGTGAAAACATCTCACATTGAGGAGTATCTCTGCGTGAAGGAGGCAATACTCTTGGATCTTCTCAGAGTTGTCAGCCATCATCGTGCTCGGCTTGCCACACCCCTCCGCACAGTTCAGAAAGTATATGGTGATGCTGACATGGAAAATGTGCCATTTGCTGACACCATTTTTGGTAGTTCTACGGCACGTACTAATCGTCCATATGTCCTCATTGAGCCATCCTATAGAATCAGTAGTGATGACAAAAGTAAGGCTTCTAATCGCTCAGCAGGTACAAATGGAGACAAGGAAGAACCTATAGCTGGGCCAACACTAACACCTGCGCAAACTGAAGATAAGGCAACTTCAACTTCCAACTCAAGTACAAGTCCCAAGACTTCGCAAATGCCAACATCTGAACTCCAAACACAGAATTTGGCATCTGATGGTTCAGCCCCAGACACCTCTGAAATACTGCAGTCCAAGAATGAGAAGATAAGATATGCAAGGAAGGAGAAGGGAGTTGATTCCAAAAATGTATCCCCAGAAAAGGCGACATCTAAGAAGTCTCCTGTTACTTCATCTGAGATGGGTGGTGGAAAGACGGATATTCCATTGACAACGTTACAGGCAAATCAAGATGGAGAGAAACCTGTTGCATCACCGTCTACAGCTCGCGCTCCCTTAGAAGAGAATATCATTCTCGGTGTTGCCTTGGAGGGCTCAAAGCGGACACTTCCCATTGAGGAAGATATGGCTCCATCTCTTATAGAATCAAAAGAATTCACCGCCAACCGTAATGGCGGCGGGTCTCCTCTTGGCACAGATGTGAAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

689

Amino Acids

75.27

Weight (kDa)

9.34

Isoelectric Point (pI)

49.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	151 - 279	1.5e-67	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	281 - 351	5.1e-12	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	356 - 442	1.7e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58200 AT1G58200
fragaria_vesca	FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370
malus_domestica	MD08G1197800.v1.1
prunus_persica	Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1
pyrus_communis	pycom15g34530
rosa_chinensis	RchiOBHm_Chr7g0236721
rosa_laevigata	RLG00000001066
rosa_multiflora	Rmu_co8258783.1_g000001 Rmu_sc0003720.1_g000014 Rmu_sc0011298.1_g000003
rosa_roxburghii	Rroxscaffold_3G00224920
rosa_rugosa	Rorug07G0298400 Rorug07G0298500 Rorug07G0298600
rosa_samantha	Rh7AG454000 Rh7BG424700 Rh7CG472300 Rh7DG441300
rosa_wichuraiana	Rw7G037640 Rw7G037670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	980
AarI	CACCTGC	1 cut(s)	1579
Acc16I	TGCGCA	3 cut(s)	392, 1139, 1575
Acc36I	ACCTGC	2 cut(s)	1511, 1579
AccB1I	GGYRCC	1 cut(s)	143
AccI	GTMKAC	1 cut(s)	1898
AccII	CGCG	1 cut(s)	1908
AciI	CCGC	6 cut(s)	659, 1357, 1960, 2019, 2034, 2037
AclI	AACGTT	1 cut(s)	1853
AclWI	GGATC	4 cut(s)	518, 531, 865, 1314
AcoI	YGGCCR	1 cut(s)	884
AcsI	RAATTY	4 cut(s)	577, 650, 1663, 2012
AcuI	CTGAAG	1 cut(s)	1602
AfaI	GTAC	6 cut(s)	105, 174, 943, 1441, 1525, 1615
AfiI	CCNNNNNNNGG	6 cut(s)	11, 134, 245, 786, 1553, 1780
AgsI	TTSAA	5 cut(s)	416, 634, 700, 923, 1599
AjnI	CCWGG	1 cut(s)	552
AleI	CACNNNNGTG	1 cut(s)	2057
AloI	GAACNNNNNNTCC	2 cut(s)	241, 273
AluBI	AGCT	8 cut(s)	11, 548, 630, 716, 797, 1130, 1553, 1904
AluI	AGCT	8 cut(s)	11, 548, 630, 716, 797, 1130, 1553, 1904
Alw21I	GWGCWC	1 cut(s)	1339
Alw26I	GTCTC	6 cut(s)	158, 656, 809, 1527, 1803, 2046
AlwI	GGATC	4 cut(s)	518, 531, 865, 1314
AlwNI	CAGNNNCTG	1 cut(s)	350
AoxI	GGCC	4 cut(s)	178, 380, 884, 1556
ApaI	GGGCCC	1 cut(s)	384
ApeKI	GCWGC	3 cut(s)	484, 740, 1171
ApoI	RAATTY	4 cut(s)	577, 650, 1663, 2012
ArsI	GACNNNNNNTTYG	2 cut(s)	1403, 1435
AseI	ATTAAT	2 cut(s)	1116, 1209
Asp700I	GAANNNNTTC	2 cut(s)	906, 1002
AspLEI	GCGC	5 cut(s)	393, 1071, 1140, 1576, 1910
AspS9I	GGNCC	5 cut(s)	179, 188, 380, 381, 1556
AsuHPI	GGTGA	4 cut(s)	960, 1391, 1884, 2008
AsuNHI	GCTAGC	1 cut(s)	548
AvaII	GGWCC	1 cut(s)	188
BaeGI	GKGCMC	1 cut(s)	384
BamHI	GGATCC	1 cut(s)	523
BanI	GGYRCC	1 cut(s)	143
BanII	GRGCYC	3 cut(s)	259, 384, 1955
BbsI	GAAGAC	1 cut(s)	1064
Bbv12I	GWGCWC	1 cut(s)	1339
BbvI	GCAGC	3 cut(s)	471, 727, 1183
BccI	CCATC	8 cut(s)	165, 547, 1335, 1477, 1670, 1813, 1865, 1999
BceAI	ACGGC	2 cut(s)	899, 1449
BciT130I	CCWGG	1 cut(s)	554
BciVI	GTATCC	1 cut(s)	1780
BcoDI	GTCTC	6 cut(s)	158, 656, 809, 1527, 1803, 2046
BfaI	CTAG	1 cut(s)	549
BfmI	CTRYAG	9 cut(s)	148, 279, 528, 726, 741, 1474, 1548, 1706, 1899
BfuAI	ACCTGC	2 cut(s)	1511, 1579
BfuI	GTATCC	1 cut(s)	1780
BglII	AGATCT	1 cut(s)	224
BisI	GCNGC	4 cut(s)	485, 741, 1172, 2035
BlpI	GCTNAGC	1 cut(s)	1516
BlsI	GCNGC	4 cut(s)	486, 742, 1173, 2036
Bme1390I	CCNGG	1 cut(s)	554
Bme18I	GGWCC	1 cut(s)	188
BmgT120I	GGNCC	5 cut(s)	179, 188, 380, 381, 1556
BmiI	GGNNCC	6 cut(s)	145, 258, 382, 383, 525, 1989
BmrFI	CCNGG	1 cut(s)	554
BmrI	ACTGGG	1 cut(s)	261
BmsI	GCATC	8 cut(s)	50, 126, 292, 378, 1080, 1372, 1679, 1898
BmtI	GCTAGC	1 cut(s)	552
BmuI	ACTGGG	1 cut(s)	261
BpiI	GAAGAC	1 cut(s)	1064
Bpu1102I	GCTNAGC	1 cut(s)	1516
BpuEI	CTTGAG	2 cut(s)	1146, 1594
BsaAI	YACGTR	3 cut(s)	176, 311, 1439
BsaI	GGTCTC	2 cut(s)	809, 2046
BsaJI	CCNNGG	1 cut(s)	1944
BsaXI	ACNNNNNCTCC	2 cut(s)	241, 271
Bsc4I	CCNNNNNNNGG	6 cut(s)	11, 134, 245, 786, 1553, 1780
Bse1I	ACTGG	3 cut(s)	191, 256, 861
Bse3DI	GCAATG	2 cut(s)	354, 507
BseBI	CCWGG	1 cut(s)	554
BseDI	CCNNGG	1 cut(s)	1944
BseGI	GGATG	3 cut(s)	65, 1108, 1469
BseLI	CCNNNNNNNGG	6 cut(s)	11, 134, 245, 786, 1553, 1780
BseMI	GCAATG	2 cut(s)	354, 507
BseMII	CTCAG	3 cut(s)	1327, 1530, 1806
BseNI	ACTGG	3 cut(s)	191, 256, 861
BseRI	GAGGAG	2 cut(s)	1286, 2034
BseSI	GKGCMC	1 cut(s)	384
BseXI	GCAGC	3 cut(s)	471, 727, 1183
BseYI	CCCAGC	3 cut(s)	766, 797, 1553
Bsh1236I	CGCG	1 cut(s)	1908
BshFI	GGCC	4 cut(s)	180, 382, 886, 1558
BshNI	GGYRCC	1 cut(s)	143
BsiHKAI	GWGCWC	1 cut(s)	1339
BslFI	GGGAC	1 cut(s)	1605
BslI	CCNNNNNNNGG	6 cut(s)	11, 134, 245, 786, 1553, 1780
BsmAI	GTCTC	6 cut(s)	158, 656, 809, 1527, 1803, 2046
BsmFI	GGGAC	1 cut(s)	1605
BsmI	GAATGC	1 cut(s)	471
BsnI	GGCC	4 cut(s)	180, 382, 886, 1558
Bso31I	GGTCTC	2 cut(s)	809, 2046
Bsp120I	GGGCCC	1 cut(s)	380
Bsp1286I	GDGCHC	4 cut(s)	259, 384, 1339, 1955
Bsp143I	GATC	5 cut(s)	224, 523, 870, 991, 1306
Bsp1720I	GCTNAGC	1 cut(s)	1516
BspACI	CCGC	6 cut(s)	659, 1357, 1960, 2019, 2034, 2037
BspANI	GGCC	4 cut(s)	180, 382, 886, 1558
BspCNI	CTCAG	3 cut(s)	1326, 1529, 1807
BspFNI	CGCG	1 cut(s)	1908
BspLI	GGNNCC	6 cut(s)	145, 258, 382, 383, 525, 1989
BspMAI	CTGCAG	4 cut(s)	152, 730, 745, 1710
BspMI	ACCTGC	2 cut(s)	1511, 1579
BspOI	GCTAGC	1 cut(s)	552
BspPI	GGATC	4 cut(s)	518, 531, 865, 1314
BspT107I	GGYRCC	1 cut(s)	143
BspTNI	GGTCTC	2 cut(s)	809, 2046
BsrDI	GCAATG	2 cut(s)	354, 507
BsrI	ACTGG	3 cut(s)	191, 256, 861
BssECI	CCNNGG	1 cut(s)	1944
BssMI	GATC	5 cut(s)	224, 523, 870, 991, 1306
BssT1I	CCWWGG	1 cut(s)	1944
Bst2UI	CCWGG	1 cut(s)	554
Bst6I	CTCTTC	2 cut(s)	1183, 1914
BstAPI	GCANNNNNTGC	1 cut(s)	1180
BstBAI	YACGTR	3 cut(s)	176, 311, 1439
BstC8I	GCNNGC	6 cut(s)	148, 550, 606, 709, 1344, 1906
BstDEI	CTNAG	7 cut(s)	915, 1049, 1313, 1516, 1791, 1815, 1915
BstEII	GGTNACC	1 cut(s)	966
BstF5I	GGATG	3 cut(s)	65, 1108, 1469
BstFNI	CGCG	1 cut(s)	1908
BstHHI	GCGC	5 cut(s)	393, 1071, 1140, 1576, 1910
BstKTI	GATC	5 cut(s)	227, 526, 873, 994, 1309
BstMAI	GTCTC	6 cut(s)	158, 656, 809, 1527, 1803, 2046
BstMBI	GATC	5 cut(s)	224, 523, 870, 991, 1306
BstMWI	GCNNNNNNNGC	7 cut(s)	356, 388, 484, 554, 713, 1180, 1343
BstNI	CCWGG	1 cut(s)	554
BstNSI	RCATGY	2 cut(s)	80, 956
BstPI	GGTNACC	1 cut(s)	966
BstSCI	CCNGG	1 cut(s)	552
BstSFI	CTRYAG	9 cut(s)	148, 279, 528, 726, 741, 1474, 1548, 1706, 1899
BstSLI	GKGCMC	1 cut(s)	384
BstUI	CGCG	1 cut(s)	1908
BstV1I	GCAGC	3 cut(s)	471, 727, 1183
BstV2I	GAAGAC	1 cut(s)	1064
BstX2I	RGATCY	3 cut(s)	224, 523, 1306
BstXI	CCANNNNNNTGG	1 cut(s)	691
BstYI	RGATCY	3 cut(s)	224, 523, 1306
BsuI	GTATCC	1 cut(s)	1780
BsuRI	GGCC	4 cut(s)	180, 382, 886, 1558
BtgZI	GCGATG	1 cut(s)	1002
BtsCI	GGATG	3 cut(s)	65, 1108, 1469
BtsI	GCAGTG	2 cut(s)	175, 750
BtsIMutI	CAGTG	5 cut(s)	175, 606, 750, 868, 1026
BveI	ACCTGC	2 cut(s)	1511, 1579
Cac8I	GCNNGC	6 cut(s)	148, 550, 606, 709, 1344, 1906
CaiI	CAGNNNCTG	1 cut(s)	350
CfoI	GCGC	5 cut(s)	393, 1071, 1140, 1576, 1910
Cfr13I	GGNCC	5 cut(s)	179, 188, 380, 381, 1556
Csp6I	GTAC	6 cut(s)	104, 173, 942, 1440, 1524, 1614
CviAII	CATG	7 cut(s)	56, 77, 134, 394, 875, 953, 1390
CviQI	GTAC	6 cut(s)	104, 173, 942, 1440, 1524, 1614
DdeI	CTNAG	7 cut(s)	915, 1049, 1313, 1516, 1791, 1815, 1915
DpnI	GATC	5 cut(s)	226, 525, 872, 993, 1308
DpnII	GATC	5 cut(s)	224, 523, 870, 991, 1306
EaeI	YGGCCR	1 cut(s)	884
Eam1104I	CTCTTC	2 cut(s)	1183, 1914
EarI	CTCTTC	2 cut(s)	1183, 1914
Eco130I	CCWWGG	1 cut(s)	1944
Eco24I	GRGCYC	3 cut(s)	259, 384, 1955
Eco31I	GGTCTC	2 cut(s)	809, 2046
Eco47I	GGWCC	1 cut(s)	188
Eco57I	CTGAAG	1 cut(s)	1602
Eco91I	GGTNACC	1 cut(s)	966
EcoO109I	RGGNCCY	1 cut(s)	381
EcoO65I	GGTNACC	1 cut(s)	966
EcoRI	GAATTC	1 cut(s)	2012
EcoRII	CCWGG	1 cut(s)	552
EcoT14I	CCWWGG	1 cut(s)	1944
EcoT38I	GRGCYC	3 cut(s)	259, 384, 1955
ErhI	CCWWGG	1 cut(s)	1944
FaeI	CATG	7 cut(s)	59, 80, 137, 397, 878, 956, 1393
FalI	AAGNNNNNCTT	2 cut(s)	1489, 1521
FaqI	GGGAC	1 cut(s)	1605
FatI	CATG	7 cut(s)	55, 76, 133, 393, 874, 952, 1389
FauI	CCCGC	1 cut(s)	2030
FauNDI	CATATG	2 cut(s)	108, 1453
FblI	GTMKAC	1 cut(s)	1898
Fnu4HI	GCNGC	4 cut(s)	485, 741, 1172, 2035
FokI	GGATG	3 cut(s)	72, 1115, 1456
FriOI	GRGCYC	3 cut(s)	259, 384, 1955
Fsp4HI	GCNGC	4 cut(s)	485, 741, 1172, 2035
FspAI	RTGCGCAY	1 cut(s)	392
FspBI	CTAG	1 cut(s)	549
FspI	TGCGCA	3 cut(s)	392, 1139, 1575
GlaI	GCGC	5 cut(s)	392, 1070, 1139, 1575, 1909
GluI	GCNGC	4 cut(s)	485, 741, 1172, 2035
GsaI	CCCAGC	3 cut(s)	770, 801, 1557
HaeIII	GGCC	4 cut(s)	180, 382, 886, 1558
HhaI	GCGC	5 cut(s)	393, 1071, 1140, 1576, 1910
Hin1II	CATG	7 cut(s)	59, 80, 137, 397, 878, 956, 1393
Hin6I	GCGC	5 cut(s)	391, 1069, 1138, 1574, 1908
HinP1I	GCGC	5 cut(s)	391, 1069, 1138, 1574, 1908
HindIII	AAGCTT	1 cut(s)	628
HphI	GGTGA	4 cut(s)	960, 1391, 1884, 2008
Hpy166II	GTNNAC	4 cut(s)	173, 1006, 1026, 1899
Hpy188I	TCNGA	9 cut(s)	267, 544, 912, 1316, 1368, 1648, 1675, 1699, 1816
Hpy188III	TCNNGA	8 cut(s)	201, 775, 836, 995, 1075, 1199, 1214, 1868
Hpy8I	GTNNAC	4 cut(s)	173, 1006, 1026, 1899
HpyAV	CCTTC	4 cut(s)	917, 1282, 1735, 1741
HpyCH4IV	ACGT	4 cut(s)	175, 310, 1438, 1853
HpyCH4V	TGCA	9 cut(s)	150, 471, 513, 728, 743, 1183, 1708, 1736, 1889
HpyF10VI	GCNNNNNNNGC	7 cut(s)	356, 388, 484, 554, 713, 1180, 1343
HpyF3I	CTNAG	7 cut(s)	915, 1049, 1313, 1516, 1791, 1815, 1915
HpySE526I	ACGT	4 cut(s)	175, 310, 1438, 1853
Hsp92II	CATG	7 cut(s)	59, 80, 137, 397, 878, 956, 1393
HspAI	GCGC	5 cut(s)	391, 1069, 1138, 1574, 1908
Kzo9I	GATC	5 cut(s)	224, 523, 870, 991, 1306
LmnI	GCTCC	4 cut(s)	262, 497, 1915, 1993
Lsp1109I	GCAGC	3 cut(s)	471, 727, 1183
LweI	GCATC	8 cut(s)	50, 126, 292, 378, 1080, 1372, 1679, 1898
MaeI	CTAG	1 cut(s)	549
MaeII	ACGT	4 cut(s)	175, 310, 1438, 1853
MaeIII	GTNAC	7 cut(s)	44, 129, 447, 668, 966, 1804, 1854
MalI	GATC	5 cut(s)	226, 525, 872, 993, 1308
MboI	GATC	5 cut(s)	224, 523, 870, 991, 1306
MflI	RGATCY	3 cut(s)	224, 523, 1306
MhlI	GDGCHC	4 cut(s)	259, 384, 1339, 1955
MluCI	AATT	8 cut(s)	332, 577, 650, 700, 975, 1113, 1663, 2012
MlyI	GAGTC	1 cut(s)	1054
MmeI	TCCRAC	2 cut(s)	413, 1629
MroXI	GAANNNNTTC	2 cut(s)	906, 1002
MseI	TTAA	5 cut(s)	164, 287, 330, 1116, 1209
MslI	CAYNNNNRTG	4 cut(s)	81, 1101, 1332, 2057
MspA1I	CMGCKG	1 cut(s)	797
MspR9I	CCNGG	1 cut(s)	554
Mva1269I	GAATGC	1 cut(s)	471
MvaI	CCWGG	1 cut(s)	554
MvnI	CGCG	1 cut(s)	1908
MwoI	GCNNNNNNNGC	7 cut(s)	356, 388, 484, 554, 713, 1180, 1343
NdeI	CATATG	2 cut(s)	108, 1453
NdeII	GATC	5 cut(s)	224, 523, 870, 991, 1306
NheI	GCTAGC	1 cut(s)	548
NlaIII	CATG	7 cut(s)	59, 80, 137, 397, 878, 956, 1393
NlaIV	GGNNCC	6 cut(s)	145, 258, 382, 383, 525, 1989
NmeAIII	GCCGAG	2 cut(s)	786, 1318
NmuCI	GTSAC	5 cut(s)	44, 129, 447, 668, 966
NsbI	TGCGCA	3 cut(s)	392, 1139, 1575
NspI	RCATGY	2 cut(s)	80, 956
OliI	CACNNNNGTG	1 cut(s)	2057
PaqCI	CACCTGC	1 cut(s)	1579
PcsI	WCGNNNNNNNCGW	1 cut(s)	1444
PctI	GAATGC	1 cut(s)	471
PdmI	GAANNNNTTC	2 cut(s)	906, 1002
PfeI	GAWTC	9 cut(s)	337, 696, 771, 907, 1045, 1219, 1479, 1757, 2003
PkrI	GCNGC	4 cut(s)	486, 742, 1173, 2036
PleI	GAGTC	1 cut(s)	1054
PpsI	GAGTC	1 cut(s)	1054
Ppu21I	YACGTR	3 cut(s)	176, 311, 1439
PshBI	ATTAAT	2 cut(s)	1116, 1209
PsiI	TTATAA	1 cut(s)	980
Psp1406I	AACGTT	1 cut(s)	1853
Psp6I	CCWGG	1 cut(s)	552
PspEI	GGTNACC	1 cut(s)	966
PspFI	CCCAGC	3 cut(s)	766, 797, 1553
PspGI	CCWGG	1 cut(s)	552
PspN4I	GGNNCC	6 cut(s)	145, 258, 382, 383, 525, 1989
PspOMI	GGGCCC	1 cut(s)	380
PspPI	GGNCC	5 cut(s)	179, 188, 380, 381, 1556
PstI	CTGCAG	4 cut(s)	152, 730, 745, 1710
PstNI	CAGNNNCTG	1 cut(s)	350
PsuI	RGATCY	3 cut(s)	224, 523, 1306
PvuII	CAGCTG	1 cut(s)	797
RsaI	GTAC	6 cut(s)	105, 174, 943, 1441, 1525, 1615
RsaNI	GTAC	6 cut(s)	104, 173, 942, 1440, 1524, 1614
RseI	CAYNNNNRTG	4 cut(s)	81, 1101, 1332, 2057
SaqAI	TTAA	5 cut(s)	164, 287, 330, 1116, 1209
SatI	GCNGC	4 cut(s)	485, 741, 1172, 2035
Sau3AI	GATC	5 cut(s)	224, 523, 870, 991, 1306
Sau96I	GGNCC	5 cut(s)	179, 188, 380, 381, 1556
SchI	GAGTC	1 cut(s)	1054
ScrFI	CCNGG	1 cut(s)	554
SduI	GDGCHC	4 cut(s)	259, 384, 1339, 1955
SfaNI	GCATC	8 cut(s)	50, 126, 292, 378, 1080, 1372, 1679, 1898
SfcI	CTRYAG	9 cut(s)	148, 279, 528, 726, 741, 1474, 1548, 1706, 1899
SinI	GGWCC	1 cut(s)	188
SmiMI	CAYNNNNRTG	4 cut(s)	81, 1101, 1332, 2057
SmlI	CTYRAG	3 cut(s)	6, 1161, 1609
SmoI	CTYRAG	3 cut(s)	6, 1161, 1609
Sse9I	AATT	8 cut(s)	332, 577, 650, 700, 975, 1113, 1663, 2012
SsiI	CCGC	6 cut(s)	659, 1357, 1960, 2019, 2034, 2037
SspI	AATATT	2 cut(s)	1120, 1207
SspMI	CTAG	1 cut(s)	549
StyD4I	CCNGG	1 cut(s)	552
StyI	CCWWGG	1 cut(s)	1944
TaiI	ACGT	4 cut(s)	178, 313, 1441, 1856
TaqI	TCGA	3 cut(s)	100, 776, 948
TasI	AATT	8 cut(s)	332, 577, 650, 700, 975, 1113, 1663, 2012
TatI	WGTACW	2 cut(s)	103, 1613
TauI	GCSGC	1 cut(s)	2037
TfiI	GAWTC	9 cut(s)	337, 696, 771, 907, 1045, 1219, 1479, 1757, 2003
Tru1I	TTAA	5 cut(s)	164, 287, 330, 1116, 1209
Tru9I	TTAA	5 cut(s)	164, 287, 330, 1116, 1209
TscAI	CASTG	5 cut(s)	175, 606, 750, 868, 1033
TseFI	GTSAC	5 cut(s)	44, 129, 447, 668, 966
TseI	GCWGC	3 cut(s)	484, 740, 1171
Tsp45I	GTSAC	5 cut(s)	44, 129, 447, 668, 966
TspDTI	ATGAA	4 cut(s)	44, 48, 915, 1800
TspGWI	ACGGA	2 cut(s)	433, 1850
TspRI	CASTG	5 cut(s)	175, 606, 750, 868, 1033
VpaK11BI	GGWCC	1 cut(s)	188
VspI	ATTAAT	2 cut(s)	1116, 1209
XapI	RAATTY	4 cut(s)	577, 650, 1663, 2012
XceI	RCATGY	2 cut(s)	80, 956
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1096
XmiI	GTMKAC	1 cut(s)	1898
XmnI	GAANNNNTTC	2 cut(s)	906, 1002
XspI	CTAG	1 cut(s)	549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.