Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0003097

Physical Location & Seq

Genomic Coordinates Forward (+)

63391494 .. 63398249

Gene Structure 6756 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG472300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGGATAATCCAGACTCGAAAATGAAAGAATTGGAACTGAGCGTCCATTTCACGGAAAGCTTCAATCTTCAGAAAAATTATCATCGTCTTCTCTCCTTTGGAGGTGTTGGACTGATTTGGGAGTTCGTGCCGGGTTCGAAAGCATTGTGCAGAGCTGTGATTCAGTTTGAGAATCAAGAAATGCCTCAAGCTGGTTCTATGAGGTTGTTGTATGAATGGAGAAGTCACAGTATTCATGGATGCCATAAACAGCCACATGCTATATTCACAAACTTCCTTTCCAGTGTAATGATCCATGCCACACGGCCATTTGTTGTCAATGAATGGATTCAGACTAAGATTCAAGGCTATGAAGTTTCTGGTACCGTCGAGCATGTGGGGTGGTGGTCACCAACAATTATAAGAGGCGATGATCGTGAAGCAGTTCACATTCCAAATCACAAGTTCACTGTGAATGTTGTGAGGAATCTTAGCCAGAAGACTCATTGGCGCATCAAGAACCACATAGCCATTAGTCATTTGGATGTCAATAAAATTAATATTATTGTAGCTGATATGCGCAAGGTTTTAGCCAAAAATCCTCAAGTAGAGCAGCAAAGGTTGCATAGAAGAGTTTTTCTGGATAATATTAATCCAGAGAATCAGGCTCTTATGATTTTGGTATCTTGCTTTGTGAAAACATCTCACATTGAGGAGTATCTCTGCGTGAAGGAGGCAATACTCTTGGATCTTCTCAGAGTTGTCAGCCATCATCGTGCTAGGCTTGCCACACCCCTCCGCACAGTTCAGAAAGTATACGGTGATGCTGACATGGAAAATGTGCCATTTGCTGACACCATTTTTGGTAGTTCTACGGCACGTACTAATCGTCCATATCTCCTCATTGAGCCATCTTACAGAATCAGTAGTGATGACAAAAGTAAGGCTTCTAATCGCTCAGCACGTACAAATGGAGACAAGGAAGAACCTAAAGCTGGGCCAACACTAACACCTGCGCAAACTGAAGATAAGGCAACTTCAACTTCCAACTCAAGTACAAGTCCCAAGACTTCGGAAATGCCAACATCTGAACCCCAAACACAGAATTTGGCATCTGATGGTTCAGCCCCAGACTCCTCTGAAATACTGCAGTCCAAGAATGAGATGATAAGATTTGCAGGGAAGGAGAAGGGAGTTGATTCCAAAAATGTATCTCCAGAAAAGGCAACATCTAAGAAGTCTCCTGTTACTTCATCTGAGATGGGTGGTCGAAAGACGGATATTCCTTTGACAACTTTACAGGCAAAACAAGATGGAGAGAAACCTGTTGCATCACCATCTACAGCTCGCGCTCCCTTAGAAGAGAATATCATTCTCGGTGTTGCCTTAGAGGGCTCAAAGCGGACACTTCCCATTGAGGAAGATATGGCTCCATCTCTTATAGAATCAAAAGAATTCACCGCCAGCCGTAATGGCAGCGGCGGGTCTCCTCCTGGCACAGATGTGAAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

497

Amino Acids

55.26

Weight (kDa)

8.19

Isoelectric Point (pI)

53.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	88 - 158	4.7e-12	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	163 - 249	1.1e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58200 AT1G58200
fragaria_vesca	FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370
malus_domestica	MD08G1197800.v1.1
prunus_persica	Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1
pyrus_communis	pycom15g34530
rosa_chinensis	RchiOBHm_Chr7g0236721
rosa_laevigata	RLG00000001066
rosa_multiflora	Rmu_co8258783.1_g000001 Rmu_sc0003720.1_g000014 Rmu_sc0011298.1_g000003
rosa_roxburghii	Rroxscaffold_3G00224920
rosa_rugosa	Rorug07G0298400 Rorug07G0298500 Rorug07G0298600
rosa_samantha	Rh7AG454000 Rh7BG424700 Rh7CG472300 Rh7DG441300
rosa_wichuraiana	Rw7G037640 Rw7G037670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	401
AarI	CACCTGC	1 cut(s)	1000
Acc16I	TGCGCA	2 cut(s)	560, 996
Acc36I	ACCTGC	1 cut(s)	1000
Acc65I	GGTACC	1 cut(s)	362
AccB1I	GGYRCC	1 cut(s)	362
AccI	GTMKAC	1 cut(s)	795
AccII	CGCG	1 cut(s)	1329
AciI	CCGC	5 cut(s)	778, 1381, 1440, 1458, 1461
AclWI	GGATC	2 cut(s)	286, 735
AcoI	YGGCCR	1 cut(s)	305
AcsI	RAATTY	2 cut(s)	1084, 1433
AcuI	CTGAAG	2 cut(s)	53, 1023
AfaI	GTAC	4 cut(s)	364, 862, 946, 1036
AfiI	CCNNNNNNNGG	3 cut(s)	52, 191, 974
AgsI	TTSAA	3 cut(s)	64, 344, 1020
AjnI	CCWGG	1 cut(s)	1471
AleI	CACNNNNGTG	1 cut(s)	1481
AloI	GAACNNNNNNTCC	2 cut(s)	27, 59
AluBI	AGCT	6 cut(s)	60, 155, 191, 551, 974, 1325
AluI	AGCT	6 cut(s)	60, 155, 191, 551, 974, 1325
Alw26I	GTCTC	3 cut(s)	948, 1224, 1470
AlwI	GGATC	2 cut(s)	286, 735
AoxI	GGCC	2 cut(s)	305, 977
ApeKI	GCWGC	2 cut(s)	592, 1455
ApoI	RAATTY	2 cut(s)	1084, 1433
ArsI	GACNNNNNNTTYG	2 cut(s)	824, 856
AseI	ATTAAT	2 cut(s)	537, 630
Asp700I	GAANNNNTTC	2 cut(s)	327, 423
Asp718I	GGTACC	1 cut(s)	362
AspLEI	GCGC	4 cut(s)	492, 561, 997, 1331
AspS9I	GGNCC	1 cut(s)	977
AsuC2I	CCSGG	1 cut(s)	132
AsuHPI	GGTGA	4 cut(s)	381, 812, 1305, 1429
AsuII	TTCGAA	1 cut(s)	137
BanI	GGYRCC	1 cut(s)	362
BanII	GRGCYC	1 cut(s)	1376
BbsI	GAAGAC	2 cut(s)	80, 485
BbvI	GCAGC	2 cut(s)	604, 1467
BccI	CCATC	7 cut(s)	756, 898, 1091, 1234, 1286, 1324, 1420
BceAI	ACGGC	3 cut(s)	320, 870, 1431
BciT130I	CCWGG	1 cut(s)	1473
BcnI	CCSGG	1 cut(s)	132
BcoDI	GTCTC	3 cut(s)	948, 1224, 1470
BfaI	CTAG	1 cut(s)	759
BfmI	CTRYAG	2 cut(s)	1127, 1320
BfuAI	ACCTGC	1 cut(s)	1000
BglI	GCCNNNNNGGC	1 cut(s)	1452
BisI	GCNGC	3 cut(s)	593, 1456, 1459
BlpI	GCTNAGC	1 cut(s)	937
BlsI	GCNGC	3 cut(s)	594, 1457, 1460
Bme1390I	CCNGG	2 cut(s)	132, 1473
BmgT120I	GGNCC	1 cut(s)	977
BmiI	GGNNCC	2 cut(s)	364, 1410
BmrFI	CCNGG	2 cut(s)	132, 1473
BmsI	GCATC	5 cut(s)	230, 501, 793, 1100, 1319
BpiI	GAAGAC	2 cut(s)	80, 485
BpmI	CTGGAG	1 cut(s)	1179
Bpu1102I	GCTNAGC	1 cut(s)	937
Bpu14I	TTCGAA	1 cut(s)	137
BpuEI	CTTGAG	3 cut(s)	171, 567, 1015
BpuMI	CCSGG	1 cut(s)	132
BsaAI	YACGTR	2 cut(s)	860, 944
BsaI	GGTCTC	1 cut(s)	1470
Bsc4I	CCNNNNNNNGG	3 cut(s)	52, 191, 974
Bse1I	ACTGG	1 cut(s)	282
BseBI	CCWGG	1 cut(s)	1473
BseGI	GGATG	2 cut(s)	245, 529
BseLI	CCNNNNNNNGG	3 cut(s)	52, 191, 974
BseMII	CTCAG	4 cut(s)	29, 748, 951, 1227
BseNI	ACTGG	1 cut(s)	282
BseRI	GAGGAG	4 cut(s)	707, 869, 1105, 1458
BseXI	GCAGC	2 cut(s)	604, 1467
BseYI	CCCAGC	1 cut(s)	974
BsgI	GTGCAG	1 cut(s)	169
Bsh1236I	CGCG	1 cut(s)	1329
BshFI	GGCC	2 cut(s)	307, 979
BshNI	GGYRCC	1 cut(s)	362
BsiSI	CCGG	1 cut(s)	131
BslFI	GGGAC	1 cut(s)	1026
BslI	CCNNNNNNNGG	3 cut(s)	52, 191, 974
BsmAI	GTCTC	3 cut(s)	948, 1224, 1470
BsmFI	GGGAC	1 cut(s)	1026
BsnI	GGCC	2 cut(s)	307, 979
Bso31I	GGTCTC	1 cut(s)	1470
Bsp119I	TTCGAA	1 cut(s)	137
Bsp1286I	GDGCHC	1 cut(s)	1376
Bsp143I	GATC	3 cut(s)	291, 412, 727
Bsp1720I	GCTNAGC	1 cut(s)	937
BspACI	CCGC	5 cut(s)	778, 1381, 1440, 1458, 1461
BspANI	GGCC	2 cut(s)	307, 979
BspCNI	CTCAG	4 cut(s)	30, 747, 950, 1228
BspFNI	CGCG	1 cut(s)	1329
BspLI	GGNNCC	2 cut(s)	364, 1410
BspMAI	CTGCAG	1 cut(s)	1131
BspMI	ACCTGC	1 cut(s)	1000
BspPI	GGATC	2 cut(s)	286, 735
BspT104I	TTCGAA	1 cut(s)	137
BspT107I	GGYRCC	1 cut(s)	362
BspTNI	GGTCTC	1 cut(s)	1470
BsrI	ACTGG	1 cut(s)	282
BssMI	GATC	3 cut(s)	291, 412, 727
BssNAI	GTATAC	1 cut(s)	796
Bst1107I	GTATAC	1 cut(s)	796
Bst2UI	CCWGG	1 cut(s)	1473
Bst4CI	ACNGT	5 cut(s)	230, 367, 451, 784, 800
Bst6I	CTCTTC	2 cut(s)	604, 1335
BstAPI	GCANNNNNTGC	1 cut(s)	601
BstBAI	YACGTR	2 cut(s)	860, 944
BstBI	TTCGAA	1 cut(s)	137
BstC8I	GCNNGC	3 cut(s)	765, 1327, 1444
BstDEI	CTNAG	9 cut(s)	38, 336, 470, 734, 937, 1212, 1236, 1336, 1366
BstEII	GGTNACC	1 cut(s)	387
BstF5I	GGATG	2 cut(s)	245, 529
BstFNI	CGCG	1 cut(s)	1329
BstHHI	GCGC	4 cut(s)	492, 561, 997, 1331
BstKTI	GATC	3 cut(s)	294, 415, 730
BstMAI	GTCTC	3 cut(s)	948, 1224, 1470
BstMBI	GATC	3 cut(s)	291, 412, 727
BstMWI	GCNNNNNNNGC	3 cut(s)	601, 764, 1452
BstNI	CCWGG	1 cut(s)	1473
BstNSI	RCATGY	2 cut(s)	260, 377
BstPI	GGTNACC	1 cut(s)	387
BstSCI	CCNGG	2 cut(s)	130, 1471
BstSFI	CTRYAG	2 cut(s)	1127, 1320
BstUI	CGCG	1 cut(s)	1329
BstV1I	GCAGC	2 cut(s)	604, 1467
BstV2I	GAAGAC	2 cut(s)	80, 485
BstX2I	RGATCY	1 cut(s)	727
BstYI	RGATCY	1 cut(s)	727
BstZ17I	GTATAC	1 cut(s)	796
BsuRI	GGCC	2 cut(s)	307, 979
BtgZI	GCGATG	1 cut(s)	423
BtsCI	GGATG	2 cut(s)	245, 529
BtsIMutI	CAGTG	2 cut(s)	289, 447
BveI	ACCTGC	1 cut(s)	1000
Cac8I	GCNNGC	3 cut(s)	765, 1327, 1444
CfoI	GCGC	4 cut(s)	492, 561, 997, 1331
Cfr13I	GGNCC	1 cut(s)	977
CseI	GACGC	1 cut(s)	31
Csp6I	GTAC	4 cut(s)	363, 861, 945, 1035
CviAII	CATG	5 cut(s)	236, 257, 296, 374, 811
CviQI	GTAC	4 cut(s)	363, 861, 945, 1035
DdeI	CTNAG	9 cut(s)	38, 336, 470, 734, 937, 1212, 1236, 1336, 1366
DpnI	GATC	3 cut(s)	293, 414, 729
DpnII	GATC	3 cut(s)	291, 412, 727
EaeI	YGGCCR	1 cut(s)	305
Eam1104I	CTCTTC	2 cut(s)	604, 1335
EarI	CTCTTC	2 cut(s)	604, 1335
Eco24I	GRGCYC	1 cut(s)	1376
Eco31I	GGTCTC	1 cut(s)	1470
Eco57I	CTGAAG	2 cut(s)	53, 1023
Eco91I	GGTNACC	1 cut(s)	387
EcoO65I	GGTNACC	1 cut(s)	387
EcoRI	GAATTC	1 cut(s)	1433
EcoRII	CCWGG	1 cut(s)	1471
EcoT38I	GRGCYC	1 cut(s)	1376
FaeI	CATG	5 cut(s)	239, 260, 299, 377, 814
FalI	AAGNNNNNCTT	2 cut(s)	910, 942
FaqI	GGGAC	1 cut(s)	1026
FatI	CATG	5 cut(s)	235, 256, 295, 373, 810
FauI	CCCGC	1 cut(s)	1454
FblI	GTMKAC	1 cut(s)	795
Fnu4HI	GCNGC	3 cut(s)	593, 1456, 1459
FokI	GGATG	2 cut(s)	252, 536
FriOI	GRGCYC	1 cut(s)	1376
Fsp4HI	GCNGC	3 cut(s)	593, 1456, 1459
FspBI	CTAG	1 cut(s)	759
FspI	TGCGCA	2 cut(s)	560, 996
GlaI	GCGC	4 cut(s)	491, 560, 996, 1330
GluI	GCNGC	3 cut(s)	593, 1456, 1459
GsaI	CCCAGC	1 cut(s)	978
GsuI	CTGGAG	1 cut(s)	1179
HaeIII	GGCC	2 cut(s)	307, 979
HapII	CCGG	1 cut(s)	131
HgaI	GACGC	1 cut(s)	31
HhaI	GCGC	4 cut(s)	492, 561, 997, 1331
Hin1II	CATG	5 cut(s)	239, 260, 299, 377, 814
Hin6I	GCGC	4 cut(s)	490, 559, 995, 1329
HinP1I	GCGC	4 cut(s)	490, 559, 995, 1329
HindIII	AAGCTT	1 cut(s)	58
HpaII	CCGG	1 cut(s)	131
HphI	GGTGA	4 cut(s)	381, 812, 1305, 1429
Hpy166II	GTNNAC	3 cut(s)	427, 447, 796
Hpy188I	TCNGA	9 cut(s)	72, 333, 737, 789, 1054, 1069, 1096, 1120, 1237
Hpy188III	TCNNGA	7 cut(s)	11, 176, 416, 496, 620, 635, 1196
Hpy8I	GTNNAC	3 cut(s)	427, 447, 796
Hpy99I	CGWCG	1 cut(s)	371
HpyAV	CCTTC	3 cut(s)	703, 1156, 1162
HpyCH4III	ACNGT	5 cut(s)	230, 367, 451, 784, 800
HpyCH4IV	ACGT	2 cut(s)	859, 943
HpyCH4V	TGCA	5 cut(s)	150, 604, 1129, 1157, 1310
HpyF10VI	GCNNNNNNNGC	3 cut(s)	601, 764, 1452
HpyF3I	CTNAG	9 cut(s)	38, 336, 470, 734, 937, 1212, 1236, 1336, 1366
HpySE526I	ACGT	2 cut(s)	859, 943
Hsp92II	CATG	5 cut(s)	239, 260, 299, 377, 814
HspAI	GCGC	4 cut(s)	490, 559, 995, 1329
KpnI	GGTACC	1 cut(s)	366
Kzo9I	GATC	3 cut(s)	291, 412, 727
LmnI	GCTCC	2 cut(s)	1336, 1414
Lsp1109I	GCAGC	2 cut(s)	604, 1467
LweI	GCATC	5 cut(s)	230, 501, 793, 1100, 1319
MaeI	CTAG	1 cut(s)	759
MaeII	ACGT	2 cut(s)	859, 943
MaeIII	GTNAC	3 cut(s)	224, 387, 1225
MalI	GATC	3 cut(s)	293, 414, 729
MboI	GATC	3 cut(s)	291, 412, 727
MboII	GAAGA	9 cut(s)	59, 80, 490, 621, 722, 974, 1016, 1352, 1412
MflI	RGATCY	1 cut(s)	727
MhlI	GDGCHC	1 cut(s)	1376
MluCI	AATT	6 cut(s)	29, 76, 396, 534, 1084, 1433
MlyI	GAGTC	3 cut(s)	8, 475, 1106
MmeI	TCCRAC	2 cut(s)	88, 1050
MroXI	GAANNNNTTC	2 cut(s)	327, 423
MseI	TTAA	2 cut(s)	537, 630
MslI	CAYNNNNRTG	3 cut(s)	522, 753, 1481
MspA1I	CMGCKG	1 cut(s)	1458
MspI	CCGG	1 cut(s)	131
MspR9I	CCNGG	2 cut(s)	132, 1473
MvaI	CCWGG	1 cut(s)	1473
MvnI	CGCG	1 cut(s)	1329
MwoI	GCNNNNNNNGC	3 cut(s)	601, 764, 1452
NciI	CCSGG	1 cut(s)	132
NdeII	GATC	3 cut(s)	291, 412, 727
NlaIII	CATG	5 cut(s)	239, 260, 299, 377, 814
NlaIV	GGNNCC	2 cut(s)	364, 1410
NmuCI	GTSAC	2 cut(s)	224, 387
NsbI	TGCGCA	2 cut(s)	560, 996
NspI	RCATGY	2 cut(s)	260, 377
NspV	TTCGAA	1 cut(s)	137
OliI	CACNNNNGTG	1 cut(s)	1481
PaqCI	CACCTGC	1 cut(s)	1000
PcsI	WCGNNNNNNNCGW	2 cut(s)	865, 940
PdmI	GAANNNNTTC	2 cut(s)	327, 423
PfeI	GAWTC	9 cut(s)	160, 172, 328, 340, 466, 640, 900, 1178, 1424
PkrI	GCNGC	3 cut(s)	594, 1457, 1460
PleI	GAGTC	3 cut(s)	8, 475, 1106
PpsI	GAGTC	3 cut(s)	8, 475, 1106
Ppu21I	YACGTR	2 cut(s)	860, 944
PshBI	ATTAAT	2 cut(s)	537, 630
PsiI	TTATAA	1 cut(s)	401
Psp6I	CCWGG	1 cut(s)	1471
PspEI	GGTNACC	1 cut(s)	387
PspFI	CCCAGC	1 cut(s)	974
PspGI	CCWGG	1 cut(s)	1471
PspN4I	GGNNCC	2 cut(s)	364, 1410
PspPI	GGNCC	1 cut(s)	977
PstI	CTGCAG	1 cut(s)	1131
PsuI	RGATCY	1 cut(s)	727
RsaI	GTAC	4 cut(s)	364, 862, 946, 1036
RsaNI	GTAC	4 cut(s)	363, 861, 945, 1035
RseI	CAYNNNNRTG	3 cut(s)	522, 753, 1481
SaqAI	TTAA	2 cut(s)	537, 630
SatI	GCNGC	3 cut(s)	593, 1456, 1459
Sau3AI	GATC	3 cut(s)	291, 412, 727
Sau96I	GGNCC	1 cut(s)	977
SchI	GAGTC	3 cut(s)	8, 475, 1106
ScrFI	CCNGG	2 cut(s)	132, 1473
SduI	GDGCHC	1 cut(s)	1376
SfaNI	GCATC	5 cut(s)	230, 501, 793, 1100, 1319
SfcI	CTRYAG	2 cut(s)	1127, 1320
SfuI	TTCGAA	1 cut(s)	137
SmiMI	CAYNNNNRTG	3 cut(s)	522, 753, 1481
SmlI	CTYRAG	3 cut(s)	186, 582, 1030
SmoI	CTYRAG	3 cut(s)	186, 582, 1030
Sse9I	AATT	6 cut(s)	29, 76, 396, 534, 1084, 1433
SsiI	CCGC	5 cut(s)	778, 1381, 1440, 1458, 1461
SspI	AATATT	2 cut(s)	541, 628
SspMI	CTAG	1 cut(s)	759
StyD4I	CCNGG	2 cut(s)	130, 1471
TaaI	ACNGT	5 cut(s)	230, 367, 451, 784, 800
TaiI	ACGT	2 cut(s)	862, 946
TaqI	TCGA	4 cut(s)	17, 137, 369, 1249
TasI	AATT	6 cut(s)	29, 76, 396, 534, 1084, 1433
TatI	WGTACW	1 cut(s)	1034
TauI	GCSGC	1 cut(s)	1461
TfiI	GAWTC	9 cut(s)	160, 172, 328, 340, 466, 640, 900, 1178, 1424
Tru1I	TTAA	2 cut(s)	537, 630
Tru9I	TTAA	2 cut(s)	537, 630
TscAI	CASTG	2 cut(s)	289, 454
TseFI	GTSAC	2 cut(s)	224, 387
TseI	GCWGC	2 cut(s)	592, 1455
Tsp45I	GTSAC	2 cut(s)	224, 387
TspDTI	ATGAA	6 cut(s)	38, 224, 228, 336, 366, 1221
TspGWI	ACGGA	2 cut(s)	68, 1271
TspRI	CASTG	2 cut(s)	289, 454
VspI	ATTAAT	2 cut(s)	537, 630
XapI	RAATTY	2 cut(s)	1084, 1433
XceI	RCATGY	2 cut(s)	260, 377
XcmI	CCANNNNNNNNNTGG	1 cut(s)	517
XmiI	GTMKAC	1 cut(s)	795
XmnI	GAANNNNTTC	2 cut(s)	327, 423
XspI	CTAG	1 cut(s)	759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.