Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0003097

Physical Location & Seq

Genomic Coordinates Forward (+)

57088107 .. 57095096

Gene Structure 6990 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G037640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGAGAGATGGCAACAAGCGAAGGTGCAGAGATGCCAGGCCCAAGGGCTGCACTCACAAAGCACTAAAAGATCTGAGTCTGGGAGAATCGTGCCGGGGTGCCTGCAGTCTCCATCTTTTAAGCAGTGTACGTGGCCCAATAGGTCCAGTATCTTCCAGATGTAAAGTTTTCTTGTGTAGATCTGTTTTTATACCAAGTGGAGGAACTGGGGCTCCTGCTCTGAAATCTACTGCTCTAGTTTTAACAAGGTCTTATGATGCTTTACGTGGAAGCCCTGTTTTCCTTAAATTGATTCAGGCAGTTGCTGTCATTGCTTTTGCTGTTTGGGGTCTTGGGCCCCTGATGCGCATGGGGAGGATTATAATTTTTCAACGGACTGATAGTAGTTGGAAGAAAAGTAGGTCACACGATGCTATGACCTCTTGCATTCAGCCTTTGCTGCTTTGGGGTGGAGCAATGCTTGTATGCAGAGCATTGGATCCTGTAGTTCTACCATCAGAAGCTAGCCAGGCTGTCAAGCAAAGACTTATAAATTTTTCACAATCATTATCAACAGTGCTGGCGTTTGCCTACTGTTTGTCAAGCTTGAACCAACAAGCACAGAAATTCTTTGCGGTGACAAGTGACCCAAGTGATACCAGAAATATGGGCTTCAATTTTGCTGGCAAAGCTGTTTATACTGCAGTTTGGGTTGCTGCAGTGTCATTGTTTATGGAGTTGCTGGGATTCTCGACCCAGAAATGGCTAACAGCTGGGGGTCTCGGCACAGTATTGCTAACCCTTGCTGGTCGTGAGATATTCACAAACTTCCTTTCCAGTGTAATGATCCATGCCACTCGGCCATTTGTTGTCAATGAATGGATTCAGACTAAGATTGAAGGCTATGAAGTTTCTGGTACTGTCGAGCATGTGGGGTGGTGGTCACCAACAATTATAAGAGGTGATGATCGTGAAGCAGTTCACATTCCAAATCACAAGTTCACTGTGAATGTTGTGAGGAATCTTAGCCAGAAGACTCATTGGCGCATCAAGAACCACATAGCCATTAGTCATTTGGATGTCAATAAAATTAATATTATTGTAGCTGATATGCGCAAAGTTTTAGCCAAAAATCCTCAAGTAGAGCAGCAAAGGTTGCATAGAAGAGTTTTTCTGGATAATATTAATCCGGAGAATCAGGCTCTTATGATTTTGGTATCTTGCTTTGTGAAAACATCTCACATTGAGGAGTATCTCTGCGTGAAGGAGGCAATACTCTTGGATCTTCTCAGAGTTGTCAGCCATCATCGTGCTCGGCTTGCCACACCCCTCCGCACAGTTCAGAAAGTATATGGTGATGCTGATATGGAAAATGTGCCATTTGCTGACACCATTTTTGGTAGTTCTACGGCACGTACTAATCGTCCATATGTCCTCATTGAGCCATCCTATAGAATCAGTAGTGATGACAAAAGTAAGGCTTCTAATCGCTCAGCAGGTACAAATGGAGACAAGGAAGAACCTATAGCTGGGCCAACACTAACACCTGCGCAAACTGAAGATAAGGCAACTTCAACTTCCAACTCAAGTACAGTCCCAAGACTTCGCAAATGCCAACATCTGAACCCCAAACACAGAATTTGGCATCTGATGGCGACATCTAAGAAGTCTCCTGTTACTTCATCTGAGATGGGTGGTGGAAAGACGGATATTCCATTGACAACTTTACAGGCAAATCAAGATGGAGAGAAACCTGTTGCATCGCCGTCTACAGCCCGCGCTCCCTTAGAAGAGAATATCATTCTCGGTGTTGCCTTGGAGGGCTCGAAGCGGACACTTCCCATTGAGGAAGATATGGCTCCATCTCTTACAGAATCAAAAGAATTCACCGCCAACCGTAATGGCGGCGGGTCTCTTGGCACAGATGTGAAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

638

Amino Acids

70.06

Weight (kDa)

9.59

Isoelectric Point (pI)

45.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	136 - 264	4.2e-66	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	266 - 336	4.6e-12	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	341 - 427	1.6e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58200 AT1G58200
fragaria_vesca	FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370
malus_domestica	MD08G1197800.v1.1
prunus_persica	Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1
pyrus_communis	pycom15g34530
rosa_chinensis	RchiOBHm_Chr7g0236721
rosa_laevigata	RLG00000001066
rosa_multiflora	Rmu_co8258783.1_g000001 Rmu_sc0003720.1_g000014 Rmu_sc0011298.1_g000003
rosa_roxburghii	Rroxscaffold_3G00224920
rosa_rugosa	Rorug07G0298400 Rorug07G0298500 Rorug07G0298600
rosa_samantha	Rh7AG454000 Rh7BG424700 Rh7CG472300 Rh7DG441300
rosa_wichuraiana	Rw7G037640 Rw7G037670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	362, 530, 935
AarI	CACCTGC	1 cut(s)	1534
Acc16I	TGCGCA	3 cut(s)	347, 1094, 1530
Acc36I	ACCTGC	2 cut(s)	1466, 1534
AccB1I	GGYRCC	1 cut(s)	98
AccI	GTMKAC	1 cut(s)	1748
AccII	CGCG	1 cut(s)	1758
AccIII	TCCGGA	1 cut(s)	1168
AciI	CCGC	7 cut(s)	614, 1312, 1756, 1810, 1869, 1884, 1887
AclWI	GGATC	4 cut(s)	473, 486, 820, 1269
AcoI	YGGCCR	1 cut(s)	839
AcsI	RAATTY	4 cut(s)	532, 605, 1617, 1862
AcuI	CTGAAG	1 cut(s)	1557
AfaI	GTAC	5 cut(s)	129, 898, 1396, 1480, 1570
AfiI	CCNNNNNNNGG	3 cut(s)	200, 741, 1508
AgsI	TTSAA	5 cut(s)	371, 589, 655, 878, 1554
AjnI	CCWGG	2 cut(s)	35, 507
AleI	CACNNNNGTG	1 cut(s)	1904
AloI	GAACNNNNNNTCC	2 cut(s)	196, 228
AluBI	AGCT	6 cut(s)	503, 585, 671, 752, 1085, 1508
AluI	AGCT	6 cut(s)	503, 585, 671, 752, 1085, 1508
Alw21I	GWGCWC	1 cut(s)	1294
Alw26I	GTCTC	5 cut(s)	113, 764, 1482, 1653, 1896
AlwI	GGATC	4 cut(s)	473, 486, 820, 1269
AlwNI	CAGNNNCTG	1 cut(s)	305
Aor13HI	TCCGGA	1 cut(s)	1168
AoxI	GGCC	5 cut(s)	38, 133, 335, 839, 1511
ApaI	GGGCCC	1 cut(s)	339
ApeKI	GCWGC	4 cut(s)	48, 439, 695, 1126
ApoI	RAATTY	4 cut(s)	532, 605, 1617, 1862
ArsI	GACNNNNNNTTYG	2 cut(s)	1358, 1390
AseI	ATTAAT	2 cut(s)	1071, 1164
Asp700I	GAANNNNTTC	2 cut(s)	861, 957
AspLEI	GCGC	5 cut(s)	348, 1026, 1095, 1531, 1760
AspS9I	GGNCC	6 cut(s)	39, 134, 143, 335, 336, 1511
AsuC2I	CCSGG	1 cut(s)	95
AsuHPI	GGTGA	5 cut(s)	628, 915, 953, 1346, 1858
AsuNHI	GCTAGC	1 cut(s)	503
AvaII	GGWCC	1 cut(s)	143
BaeGI	GKGCMC	1 cut(s)	339
BamHI	GGATCC	1 cut(s)	478
BanI	GGYRCC	1 cut(s)	98
BanII	GRGCYC	3 cut(s)	214, 339, 1805
BbsI	GAAGAC	1 cut(s)	1019
Bbv12I	GWGCWC	1 cut(s)	1294
BbvI	GCAGC	4 cut(s)	35, 426, 682, 1138
BccI	CCATC	9 cut(s)	2, 120, 502, 1290, 1432, 1624, 1663, 1715, 1849
BceAI	ACGGC	2 cut(s)	1404, 1729
BciT130I	CCWGG	2 cut(s)	37, 509
BcnI	CCSGG	1 cut(s)	95
BcoDI	GTCTC	5 cut(s)	113, 764, 1482, 1653, 1896
BfaI	CTAG	2 cut(s)	236, 504
BfmI	CTRYAG	7 cut(s)	103, 483, 681, 696, 1429, 1503, 1749
BfuAI	ACCTGC	2 cut(s)	1466, 1534
BglII	AGATCT	2 cut(s)	70, 179
BisI	GCNGC	5 cut(s)	49, 440, 696, 1127, 1885
BlpI	GCTNAGC	1 cut(s)	1471
BlsI	GCNGC	5 cut(s)	50, 441, 697, 1128, 1886
Bme1390I	CCNGG	3 cut(s)	37, 95, 509
Bme18I	GGWCC	1 cut(s)	143
BmgT120I	GGNCC	6 cut(s)	39, 134, 143, 335, 336, 1511
BmiI	GGNNCC	6 cut(s)	100, 213, 337, 338, 480, 1839
BmrFI	CCNGG	3 cut(s)	37, 95, 509
BmrI	ACTGGG	1 cut(s)	216
BmsI	GCATC	8 cut(s)	22, 247, 333, 400, 1035, 1327, 1633, 1748
BmtI	GCTAGC	1 cut(s)	507
BmuI	ACTGGG	1 cut(s)	216
BpiI	GAAGAC	1 cut(s)	1019
Bpu1102I	GCTNAGC	1 cut(s)	1471
BpuEI	CTTGAG	2 cut(s)	1101, 1549
BpuMI	CCSGG	1 cut(s)	95
BsaAI	YACGTR	3 cut(s)	131, 266, 1394
BsaI	GGTCTC	2 cut(s)	764, 1896
BsaJI	CCNNGG	3 cut(s)	42, 94, 1794
BsaWI	WCCGGW	1 cut(s)	1168
BsaXI	ACNNNNNCTCC	2 cut(s)	196, 226
Bsc4I	CCNNNNNNNGG	3 cut(s)	200, 741, 1508
Bse1I	ACTGG	3 cut(s)	146, 211, 816
Bse3DI	GCAATG	2 cut(s)	309, 462
BseAI	TCCGGA	1 cut(s)	1168
BseBI	CCWGG	2 cut(s)	37, 509
BseDI	CCNNGG	3 cut(s)	42, 94, 1794
BseGI	GGATG	2 cut(s)	1063, 1424
BseLI	CCNNNNNNNGG	3 cut(s)	200, 741, 1508
BseMI	GCAATG	2 cut(s)	309, 462
BseMII	CTCAG	4 cut(s)	65, 1282, 1485, 1656
BseNI	ACTGG	3 cut(s)	146, 211, 816
BseRI	GAGGAG	1 cut(s)	1241
BseSI	GKGCMC	1 cut(s)	339
BseXI	GCAGC	4 cut(s)	35, 426, 682, 1138
BseYI	CCCAGC	3 cut(s)	721, 752, 1508
BsgI	GTGCAG	2 cut(s)	34, 46
Bsh1236I	CGCG	1 cut(s)	1758
BshFI	GGCC	5 cut(s)	40, 135, 337, 841, 1513
BshNI	GGYRCC	1 cut(s)	98
BsiHKAI	GWGCWC	1 cut(s)	1294
BsiSI	CCGG	2 cut(s)	94, 1169
BslFI	GGGAC	1 cut(s)	1559
BslI	CCNNNNNNNGG	3 cut(s)	200, 741, 1508
BsmAI	GTCTC	5 cut(s)	113, 764, 1482, 1653, 1896
BsmFI	GGGAC	1 cut(s)	1559
BsmI	GAATGC	1 cut(s)	426
BsnI	GGCC	5 cut(s)	40, 135, 337, 841, 1513
Bso31I	GGTCTC	2 cut(s)	764, 1896
Bsp120I	GGGCCC	1 cut(s)	335
Bsp1286I	GDGCHC	4 cut(s)	214, 339, 1294, 1805
Bsp13I	TCCGGA	1 cut(s)	1168
Bsp143I	GATC	6 cut(s)	70, 179, 478, 825, 946, 1261
Bsp1720I	GCTNAGC	1 cut(s)	1471
BspACI	CCGC	7 cut(s)	614, 1312, 1756, 1810, 1869, 1884, 1887
BspANI	GGCC	5 cut(s)	40, 135, 337, 841, 1513
BspCNI	CTCAG	4 cut(s)	66, 1281, 1484, 1657
BspEI	TCCGGA	1 cut(s)	1168
BspFNI	CGCG	1 cut(s)	1758
BspLI	GGNNCC	6 cut(s)	100, 213, 337, 338, 480, 1839
BspMAI	CTGCAG	3 cut(s)	107, 685, 700
BspMI	ACCTGC	2 cut(s)	1466, 1534
BspOI	GCTAGC	1 cut(s)	507
BspPI	GGATC	4 cut(s)	473, 486, 820, 1269
BspT107I	GGYRCC	1 cut(s)	98
BspTNI	GGTCTC	2 cut(s)	764, 1896
BsrDI	GCAATG	2 cut(s)	309, 462
BsrI	ACTGG	3 cut(s)	146, 211, 816
BssECI	CCNNGG	3 cut(s)	42, 94, 1794
BssMI	GATC	6 cut(s)	70, 179, 478, 825, 946, 1261
BssT1I	CCWWGG	2 cut(s)	42, 1794
Bst2UI	CCWGG	2 cut(s)	37, 509
Bst4CI	ACNGT	8 cut(s)	556, 575, 769, 901, 985, 1318, 1573, 1877
Bst6I	CTCTTC	2 cut(s)	1138, 1764
BstAPI	GCANNNNNTGC	1 cut(s)	1135
BstBAI	YACGTR	3 cut(s)	131, 266, 1394
BstC8I	GCNNGC	6 cut(s)	103, 505, 561, 664, 1299, 1756
BstDEI	CTNAG	8 cut(s)	74, 870, 1004, 1268, 1471, 1641, 1665, 1765
BstEII	GGTNACC	1 cut(s)	921
BstF5I	GGATG	2 cut(s)	1063, 1424
BstFNI	CGCG	1 cut(s)	1758
BstHHI	GCGC	5 cut(s)	348, 1026, 1095, 1531, 1760
BstKTI	GATC	6 cut(s)	73, 182, 481, 828, 949, 1264
BstMAI	GTCTC	5 cut(s)	113, 764, 1482, 1653, 1896
BstMBI	GATC	6 cut(s)	70, 179, 478, 825, 946, 1261
BstMWI	GCNNNNNNNGC	7 cut(s)	311, 343, 439, 509, 668, 1135, 1298
BstNI	CCWGG	2 cut(s)	37, 509
BstNSI	RCATGY	1 cut(s)	911
BstPI	GGTNACC	1 cut(s)	921
BstSCI	CCNGG	3 cut(s)	35, 93, 507
BstSFI	CTRYAG	7 cut(s)	103, 483, 681, 696, 1429, 1503, 1749
BstSLI	GKGCMC	1 cut(s)	339
BstUI	CGCG	1 cut(s)	1758
BstV1I	GCAGC	4 cut(s)	35, 426, 682, 1138
BstV2I	GAAGAC	1 cut(s)	1019
BstX2I	RGATCY	4 cut(s)	70, 179, 478, 1261
BstXI	CCANNNNNNTGG	1 cut(s)	646
BstYI	RGATCY	4 cut(s)	70, 179, 478, 1261
BsuRI	GGCC	5 cut(s)	40, 135, 337, 841, 1513
BtgZI	GCGATG	1 cut(s)	1725
BtsCI	GGATG	2 cut(s)	1063, 1424
BtsI	GCAGTG	2 cut(s)	130, 705
BtsIMutI	CAGTG	5 cut(s)	130, 561, 705, 823, 981
BveI	ACCTGC	2 cut(s)	1466, 1534
Cac8I	GCNNGC	6 cut(s)	103, 505, 561, 664, 1299, 1756
CaiI	CAGNNNCTG	1 cut(s)	305
CfoI	GCGC	5 cut(s)	348, 1026, 1095, 1531, 1760
Cfr13I	GGNCC	6 cut(s)	39, 134, 143, 335, 336, 1511
Csp6I	GTAC	5 cut(s)	128, 897, 1395, 1479, 1569
CviAII	CATG	3 cut(s)	349, 830, 908
CviQI	GTAC	5 cut(s)	128, 897, 1395, 1479, 1569
DdeI	CTNAG	8 cut(s)	74, 870, 1004, 1268, 1471, 1641, 1665, 1765
DpnI	GATC	6 cut(s)	72, 181, 480, 827, 948, 1263
DpnII	GATC	6 cut(s)	70, 179, 478, 825, 946, 1261
EaeI	YGGCCR	1 cut(s)	839
Eam1104I	CTCTTC	2 cut(s)	1138, 1764
EarI	CTCTTC	2 cut(s)	1138, 1764
Eco130I	CCWWGG	2 cut(s)	42, 1794
Eco24I	GRGCYC	3 cut(s)	214, 339, 1805
Eco31I	GGTCTC	2 cut(s)	764, 1896
Eco47I	GGWCC	1 cut(s)	143
Eco57I	CTGAAG	1 cut(s)	1557
Eco91I	GGTNACC	1 cut(s)	921
EcoO109I	RGGNCCY	1 cut(s)	336
EcoO65I	GGTNACC	1 cut(s)	921
EcoRI	GAATTC	1 cut(s)	1862
EcoRII	CCWGG	2 cut(s)	35, 507
EcoT14I	CCWWGG	2 cut(s)	42, 1794
EcoT38I	GRGCYC	3 cut(s)	214, 339, 1805
ErhI	CCWWGG	2 cut(s)	42, 1794
FaeI	CATG	3 cut(s)	352, 833, 911
FalI	AAGNNNNNCTT	2 cut(s)	1444, 1476
FaqI	GGGAC	1 cut(s)	1559
FatI	CATG	3 cut(s)	348, 829, 907
FauI	CCCGC	2 cut(s)	1763, 1880
FauNDI	CATATG	1 cut(s)	1408
FblI	GTMKAC	1 cut(s)	1748
Fnu4HI	GCNGC	5 cut(s)	49, 440, 696, 1127, 1885
FokI	GGATG	2 cut(s)	1070, 1411
FriOI	GRGCYC	3 cut(s)	214, 339, 1805
Fsp4HI	GCNGC	5 cut(s)	49, 440, 696, 1127, 1885
FspAI	RTGCGCAY	1 cut(s)	347
FspBI	CTAG	2 cut(s)	236, 504
FspI	TGCGCA	3 cut(s)	347, 1094, 1530
GlaI	GCGC	5 cut(s)	347, 1025, 1094, 1530, 1759
GluI	GCNGC	5 cut(s)	49, 440, 696, 1127, 1885
GsaI	CCCAGC	3 cut(s)	725, 756, 1512
HaeIII	GGCC	5 cut(s)	40, 135, 337, 841, 1513
HapII	CCGG	2 cut(s)	94, 1169
HhaI	GCGC	5 cut(s)	348, 1026, 1095, 1531, 1760
Hin1II	CATG	3 cut(s)	352, 833, 911
Hin6I	GCGC	5 cut(s)	346, 1024, 1093, 1529, 1758
HinP1I	GCGC	5 cut(s)	346, 1024, 1093, 1529, 1758
HindIII	AAGCTT	1 cut(s)	583
HpaII	CCGG	2 cut(s)	94, 1169
HphI	GGTGA	5 cut(s)	628, 915, 953, 1346, 1858
Hpy166II	GTNNAC	4 cut(s)	128, 961, 981, 1749
Hpy188I	TCNGA	9 cut(s)	75, 222, 499, 867, 1271, 1323, 1602, 1629, 1666
Hpy188III	TCNNGA	8 cut(s)	156, 730, 791, 950, 1030, 1154, 1169, 1718
Hpy8I	GTNNAC	4 cut(s)	128, 961, 981, 1749
HpyAV	CCTTC	3 cut(s)	15, 872, 1237
HpyCH4III	ACNGT	8 cut(s)	556, 575, 769, 901, 985, 1318, 1573, 1877
HpyCH4IV	ACGT	3 cut(s)	130, 265, 1393
HpyCH4V	TGCA	9 cut(s)	27, 51, 105, 426, 468, 683, 698, 1138, 1739
HpyF10VI	GCNNNNNNNGC	7 cut(s)	311, 343, 439, 509, 668, 1135, 1298
HpyF3I	CTNAG	8 cut(s)	74, 870, 1004, 1268, 1471, 1641, 1665, 1765
HpySE526I	ACGT	3 cut(s)	130, 265, 1393
Hsp92II	CATG	3 cut(s)	352, 833, 911
HspAI	GCGC	5 cut(s)	346, 1024, 1093, 1529, 1758
Kpn2I	TCCGGA	1 cut(s)	1168
Kzo9I	GATC	6 cut(s)	70, 179, 478, 825, 946, 1261
LmnI	GCTCC	4 cut(s)	217, 452, 1765, 1843
Lsp1109I	GCAGC	4 cut(s)	35, 426, 682, 1138
LweI	GCATC	8 cut(s)	22, 247, 333, 400, 1035, 1327, 1633, 1748
MaeI	CTAG	2 cut(s)	236, 504
MaeII	ACGT	3 cut(s)	130, 265, 1393
MaeIII	GTNAC	5 cut(s)	402, 616, 623, 921, 1654
MalI	GATC	6 cut(s)	72, 181, 480, 827, 948, 1263
MboI	GATC	6 cut(s)	70, 179, 478, 825, 946, 1261
MboII	GAAGA	9 cut(s)	144, 403, 1024, 1155, 1256, 1508, 1550, 1781, 1841
MflI	RGATCY	4 cut(s)	70, 179, 478, 1261
MhlI	GDGCHC	4 cut(s)	214, 339, 1294, 1805
MluCI	AATT	9 cut(s)	287, 363, 532, 605, 655, 930, 1068, 1617, 1862
MlyI	GAGTC	2 cut(s)	85, 1009
MmeI	TCCRAC	2 cut(s)	368, 1584
MroI	TCCGGA	1 cut(s)	1168
MroXI	GAANNNNTTC	2 cut(s)	861, 957
MseI	TTAA	5 cut(s)	119, 242, 285, 1071, 1164
MslI	CAYNNNNRTG	3 cut(s)	1056, 1287, 1904
MspA1I	CMGCKG	1 cut(s)	752
MspI	CCGG	2 cut(s)	94, 1169
MspR9I	CCNGG	3 cut(s)	37, 95, 509
Mva1269I	GAATGC	1 cut(s)	426
MvaI	CCWGG	2 cut(s)	37, 509
MvnI	CGCG	1 cut(s)	1758
MwoI	GCNNNNNNNGC	7 cut(s)	311, 343, 439, 509, 668, 1135, 1298
NciI	CCSGG	1 cut(s)	95
NdeI	CATATG	1 cut(s)	1408
NdeII	GATC	6 cut(s)	70, 179, 478, 825, 946, 1261
NheI	GCTAGC	1 cut(s)	503
NlaIII	CATG	3 cut(s)	352, 833, 911
NlaIV	GGNNCC	6 cut(s)	100, 213, 337, 338, 480, 1839
NmeAIII	GCCGAG	3 cut(s)	741, 817, 1273
NmuCI	GTSAC	4 cut(s)	402, 616, 623, 921
NsbI	TGCGCA	3 cut(s)	347, 1094, 1530
NspI	RCATGY	1 cut(s)	911
OliI	CACNNNNGTG	1 cut(s)	1904
PaqCI	CACCTGC	1 cut(s)	1534
PcsI	WCGNNNNNNNCGW	1 cut(s)	1399
PctI	GAATGC	1 cut(s)	426
PdmI	GAANNNNTTC	2 cut(s)	861, 957
PfeI	GAWTC	8 cut(s)	86, 292, 726, 862, 1000, 1174, 1434, 1853
PkrI	GCNGC	5 cut(s)	50, 441, 697, 1128, 1886
PleI	GAGTC	2 cut(s)	84, 1009
PpsI	GAGTC	2 cut(s)	84, 1009
Ppu21I	YACGTR	3 cut(s)	131, 266, 1394
PshBI	ATTAAT	2 cut(s)	1071, 1164
PsiI	TTATAA	3 cut(s)	362, 530, 935
Psp6I	CCWGG	2 cut(s)	35, 507
PspEI	GGTNACC	1 cut(s)	921
PspFI	CCCAGC	3 cut(s)	721, 752, 1508
PspGI	CCWGG	2 cut(s)	35, 507
PspN4I	GGNNCC	6 cut(s)	100, 213, 337, 338, 480, 1839
PspOMI	GGGCCC	1 cut(s)	335
PspPI	GGNCC	6 cut(s)	39, 134, 143, 335, 336, 1511
PstI	CTGCAG	3 cut(s)	107, 685, 700
PstNI	CAGNNNCTG	1 cut(s)	305
PsuI	RGATCY	4 cut(s)	70, 179, 478, 1261
PvuII	CAGCTG	1 cut(s)	752
RsaI	GTAC	5 cut(s)	129, 898, 1396, 1480, 1570
RsaNI	GTAC	5 cut(s)	128, 897, 1395, 1479, 1569
RseI	CAYNNNNRTG	3 cut(s)	1056, 1287, 1904
SaqAI	TTAA	5 cut(s)	119, 242, 285, 1071, 1164
SatI	GCNGC	5 cut(s)	49, 440, 696, 1127, 1885
Sau3AI	GATC	6 cut(s)	70, 179, 478, 825, 946, 1261
Sau96I	GGNCC	6 cut(s)	39, 134, 143, 335, 336, 1511
SchI	GAGTC	2 cut(s)	85, 1009
ScrFI	CCNGG	3 cut(s)	37, 95, 509
SduI	GDGCHC	4 cut(s)	214, 339, 1294, 1805
SfaNI	GCATC	8 cut(s)	22, 247, 333, 400, 1035, 1327, 1633, 1748
SfcI	CTRYAG	7 cut(s)	103, 483, 681, 696, 1429, 1503, 1749
SinI	GGWCC	1 cut(s)	143
SmiMI	CAYNNNNRTG	3 cut(s)	1056, 1287, 1904
SmlI	CTYRAG	2 cut(s)	1116, 1564
SmoI	CTYRAG	2 cut(s)	1116, 1564
Sse9I	AATT	9 cut(s)	287, 363, 532, 605, 655, 930, 1068, 1617, 1862
SsiI	CCGC	7 cut(s)	614, 1312, 1756, 1810, 1869, 1884, 1887
SspI	AATATT	2 cut(s)	1075, 1162
SspMI	CTAG	2 cut(s)	236, 504
StyD4I	CCNGG	3 cut(s)	35, 93, 507
StyI	CCWWGG	2 cut(s)	42, 1794
TaaI	ACNGT	8 cut(s)	556, 575, 769, 901, 985, 1318, 1573, 1877
TaiI	ACGT	3 cut(s)	133, 268, 1396
TaqI	TCGA	3 cut(s)	731, 903, 1805
TasI	AATT	9 cut(s)	287, 363, 532, 605, 655, 930, 1068, 1617, 1862
TatI	WGTACW	1 cut(s)	1568
TauI	GCSGC	1 cut(s)	1887
TfiI	GAWTC	8 cut(s)	86, 292, 726, 862, 1000, 1174, 1434, 1853
Tru1I	TTAA	5 cut(s)	119, 242, 285, 1071, 1164
Tru9I	TTAA	5 cut(s)	119, 242, 285, 1071, 1164
TscAI	CASTG	5 cut(s)	130, 561, 705, 823, 988
TseFI	GTSAC	4 cut(s)	402, 616, 623, 921
TseI	GCWGC	4 cut(s)	48, 439, 695, 1126
Tsp45I	GTSAC	4 cut(s)	402, 616, 623, 921
TspDTI	ATGAA	3 cut(s)	870, 900, 1650
TspGWI	ACGGA	2 cut(s)	388, 1700
TspRI	CASTG	5 cut(s)	130, 561, 705, 823, 988
VpaK11BI	GGWCC	1 cut(s)	143
VspI	ATTAAT	2 cut(s)	1071, 1164
XapI	RAATTY	4 cut(s)	532, 605, 1617, 1862
XceI	RCATGY	1 cut(s)	911
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1051
XmiI	GTMKAC	1 cut(s)	1748
XmnI	GAANNNNTTC	2 cut(s)	861, 957
XspI	CTAG	2 cut(s)	236, 504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.