Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003097

Physical Location & Seq

Genomic Coordinates Reverse (-)

25638827 .. 25644586

Gene Structure 5760 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1197800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGCCTCATGCGGCTGCTACGAGGTTTTTGTATGATTGGAGAAGTCACAATAATCGTGGATGCCGTAATCCAGATGCTTGTCTTGTAGGAAAGGGTCGAGTGCATATGGTTAGCATCAATCTTCCGTCACATGGTTTGCGACAGGGTGCCTGCAGTCTCCATCTTTTAAGCAGTGTACGTGGCCCAATAGGTCCAGTGTCTTCCAGATGCAATGTCTTCGTTTGCAGATCTGTTTTAGGTCCAGGTGGAGGAAACGGGGTTCCTGTTCTGAAATCTGCTACTCTTGTTTTGACAAGGTCCTATGATACTTTACGTGGAAGCCCTGCTTTACTTAAATTGATCCCAGCAGTGGCTATCATTGCTTTTGCTGTTTGGGGTATTGGGCCACTATTGCGTCTCGGCAGGGTTATTTTTCTTCGACGGAATGATAATACTTGGATGAAAAGTAGGTCACACTATGTCATGACTTCTTACCTTCAGCCCTTGCTTCTCTGGACTGGAGCGACACTTTTATGCAGAGCATTGGATCCTGTAGTCCTACCTTCAGAAGCTAGCCAGGCTGTCAAGCAACGTCTTATAAATTTTACACAATCATTATCAACAGTGTTGGCATTTGCCTATTGTTTGTCAAGCTTAATTCAACAAGCACAAAAGTTCTTTATGGAGACAAGAGACCCAAGTGACACAAGAAATATGGGCTTCAATTTCGCTGGAAAGGCGGTTTATTCTGCAGTTTGGGTTGCTGCGGTATCATTGTTCATGGAATTGCTGGGATTCTCAACCCAGAAATGGGTAACGGCTGGGGGTCTTGGCACAGTATTGCTTACCCTTGCTGGTCGTGAGATATTCACAAACTTCCTTTCAAGTGTAATGATCCATGCCACACGCCCATTTGTTGTTAACGAATGGATCCAGACTAAGATTGAAGGTTATGAAGTTTCTGGTACTGTTGAGCATGTGGGTTGGTGGTCTCCAACAATTATAAGGGGCGATGATCGTGAAGCAGTTCACATTCCAAATCACAAGTTCACTGTAAATGTTGTCCGGAATCTTAGCCAGAAAAGTCATTGGCGAATCAAGACTCACCTCGCAGTCAGTCACTTGGATGTAAATAAAATTAATACTATAGTAGCTGATATGCGCAAGGTTCTGGCAAAAAATCCTCAAGTAGAGCAGCAAAGGTTGCATAGAAGAGTTTTTCTGGACAATATTAATCCAGAAAATCAGGCTCTTATGATTTTGGTGTCTTGCTTTGTTAAAACATCTCATATTGAGGAGTATCTCTGCGTCAAGGAGGCAATACTTTTGGATCTTCTCAGAGTTATTAGCCATCACCATGCCCGGCTTGCCACACCCATCCGCACAGTCCAGAAATATTACAGTGAGGCTGACTTGGAAAATGTGCCATTCGCTGACACCATTTTCACTCGTTCTAGGGCAGCCACTAATCGTCCGTATCTACTTATTGAACCATCTTACAAAATCAGTAGTGACGACAAAGGCAAGGCTTCAACTCGCACAATGCGTACAAATGGAGAAAAACAGGCCCAAGCTGAAGCAGCCTCACCATCTGACTCCAAGGGATCAGATGCTAAAGCTGGGCCAGCCGTAACACATGCACAAACTGACGATAAGGTTGCAGCGACTTCAAGTTCCAACTCAAGTACAACCTCTAAGACTTCTGAAATGCCAACACCCGAACCCCAAACTTGGAATCCCACATCTGATGATTCAGTTAGAAACAACTCCGAAATGCTGCAGTCCAAGAACGAGAGTATAAAATATCCAGGGAAGGAGACAGGAGCTGATTCCAAAGGTGTGTCTCCACAAAAGGTGACGGCTAAGAAGCCTCATGTTGCTTCACCCGAGACTGGTAGCGAAAAAGCAGATGTTCCTTTGACCACTACACAGCCAAAGCAAGATGGCGAGAAGCCTGTTGCAGCACCATCGACAGCTAGGCCTCCATTAGAAGAGAATATTATCCTTGGCGTTGCTTTGGAGGGCTCAAAGCGAACGCTTCCAATCGAGGAGGATATGACTCCATCTCCTAATGCAGTATCAAAAGAATTCAGTGGCAGCCGTAATGGTGGCGGGTCTCCTTCCGTTGGCACGGATGTGAAGGATGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

710

Amino Acids

77.51

Weight (kDa)

9.44

Isoelectric Point (pI)

46.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	152 - 280	2.5e-71	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	282 - 352	5.3e-12	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	357 - 443	1.9e-45	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58200 AT1G58200
fragaria_vesca	FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370 FvH4_5g35370
malus_domestica	MD08G1197800.v1.1
prunus_persica	Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1 Prupe.1G531900_v2.0.a1
pyrus_communis	pycom15g34530
rosa_chinensis	RchiOBHm_Chr7g0236721
rosa_laevigata	RLG00000001066
rosa_multiflora	Rmu_co8258783.1_g000001 Rmu_sc0003720.1_g000014 Rmu_sc0011298.1_g000003
rosa_roxburghii	Rroxscaffold_3G00224920
rosa_rugosa	Rorug07G0298400 Rorug07G0298500 Rorug07G0298600
rosa_samantha	Rh7AG454000 Rh7BG424700 Rh7CG472300 Rh7DG441300
rosa_wichuraiana	Rw7G037640 Rw7G037670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	578, 983
Acc16I	TGCGCA	1 cut(s)	1142
AccB1I	GGYRCC	1 cut(s)	146
AccIII	TCCGGA	1 cut(s)	1044
AciI	CCGC	5 cut(s)	11, 719, 746, 1360, 2091
AclWI	GGATC	8 cut(s)	334, 521, 534, 868, 904, 917, 1317, 1591
AcsI	RAATTY	2 cut(s)	580, 2066
AcuI	CTGAAG	3 cut(s)	461, 528, 1575
AfaI	GTAC	4 cut(s)	177, 946, 1528, 1666
AfiI	CCNNNNNNNGG	8 cut(s)	131, 248, 349, 789, 790, 1710, 1871, 2105
AgsI	TTSAA	7 cut(s)	641, 703, 864, 926, 1469, 1512, 1650
AjnI	CCWGG	3 cut(s)	241, 555, 1786
AleI	CACNNNNGTG	1 cut(s)	2114
AloI	GAACNNNNNNTCC	2 cut(s)	243, 275
AluBI	AGCT	7 cut(s)	551, 633, 1133, 1553, 1598, 1805, 1955
AluI	AGCT	7 cut(s)	551, 633, 1133, 1553, 1598, 1805, 1955
Alw26I	GTCTC	9 cut(s)	161, 401, 659, 666, 975, 1790, 1827, 1862, 2100
AlwI	GGATC	8 cut(s)	334, 521, 534, 868, 904, 917, 1317, 1591
AlwNI	CAGNNNCTG	1 cut(s)	1805
Ama87I	CYCGRG	1 cut(s)	1865
Aor13HI	TCCGGA	1 cut(s)	1044
AoxI	GGCC	5 cut(s)	181, 383, 1545, 1601, 1958
ApeKI	GCWGC	9 cut(s)	14, 743, 1174, 1439, 1559, 1640, 1756, 1940, 2076
ApoI	RAATTY	2 cut(s)	580, 2066
AseI	ATTAAT	2 cut(s)	1119, 1212
Asp700I	GAANNNNTTC	1 cut(s)	1005
AspLEI	GCGC	1 cut(s)	1143
AspS9I	GGNCC	7 cut(s)	182, 191, 239, 297, 383, 1546, 1601
AsuC2I	CCSGG	1 cut(s)	1342
AsuHPI	GGTGA	5 cut(s)	1076, 1325, 1557, 1846, 1854
AsuNHI	GCTAGC	1 cut(s)	551
AvaI	CYCGRG	1 cut(s)	1865
AvaII	GGWCC	3 cut(s)	191, 239, 297
BamHI	GGATCC	2 cut(s)	526, 909
BanI	GGYRCC	1 cut(s)	146
BanII	GRGCYC	1 cut(s)	2006
BbsI	GAAGAC	2 cut(s)	192, 208
BbvI	GCAGC	8 cut(s)	730, 1186, 1451, 1571, 1652, 1743, 1952, 2088
BccI	CCATC	9 cut(s)	168, 1338, 1364, 1480, 1576, 1916, 1954, 2050, 2117
BceAI	ACGGC	5 cut(s)	48, 813, 1592, 1854, 2064
BcgI	CGANNNNNNTGC	6 cut(s)	199, 233, 1619, 1653, 1929, 1963
BciT130I	CCWGG	3 cut(s)	243, 557, 1788
BcnI	CCSGG	1 cut(s)	1342
BcoDI	GTCTC	9 cut(s)	161, 401, 659, 666, 975, 1790, 1827, 1862, 2100
BfaI	CTAG	3 cut(s)	552, 1434, 1956
BfmI	CTRYAG	5 cut(s)	151, 531, 729, 1125, 1757
BglII	AGATCT	1 cut(s)	227
Bme1390I	CCNGG	4 cut(s)	243, 557, 1342, 1788
Bme18I	GGWCC	3 cut(s)	191, 239, 297
BmeT110I	CYCGRG	1 cut(s)	1865
BmgT120I	GGNCC	7 cut(s)	182, 191, 239, 297, 383, 1546, 1601
BmiI	GGNNCC	4 cut(s)	148, 261, 528, 911
BmrFI	CCNGG	4 cut(s)	243, 557, 1342, 1788
BmsI	GCATC	5 cut(s)	50, 64, 123, 197, 1579
BmtI	GCTAGC	1 cut(s)	555
BpiI	GAAGAC	2 cut(s)	192, 208
BpmI	CTGGAG	1 cut(s)	519
BpuEI	CTTGAG	2 cut(s)	1149, 1645
BpuMI	CCSGG	1 cut(s)	1342
BsaAI	YACGTR	2 cut(s)	179, 314
BsaI	GGTCTC	3 cut(s)	666, 975, 2100
BsaJI	CCNNGG	3 cut(s)	1578, 1787, 1984
BsaWI	WCCGGW	1 cut(s)	1044
BsaXI	ACNNNNNCTCC	2 cut(s)	2029, 2059
Bsc4I	CCNNNNNNNGG	8 cut(s)	131, 248, 349, 789, 790, 1710, 1871, 2105
Bse1I	ACTGG	3 cut(s)	194, 502, 1876
Bse3DI	GCAATG	2 cut(s)	217, 357
BseAI	TCCGGA	1 cut(s)	1044
BseBI	CCWGG	3 cut(s)	243, 557, 1788
BseDI	CCNNGG	3 cut(s)	1578, 1787, 1984
BseGI	GGATG	6 cut(s)	65, 444, 1111, 1356, 2119, 2128
BseLI	CCNNNNNNNGG	8 cut(s)	131, 248, 349, 789, 790, 1710, 1871, 2105
BseMI	GCAATG	2 cut(s)	217, 357
BseMII	CTCAG	1 cut(s)	1330
BseNI	ACTGG	3 cut(s)	194, 502, 1876
BseRI	GAGGAG	2 cut(s)	1289, 2042
BseXI	GCAGC	8 cut(s)	730, 1186, 1451, 1571, 1652, 1743, 1952, 2088
BseYI	CCCAGC	4 cut(s)	343, 769, 800, 1598
BshFI	GGCC	5 cut(s)	183, 385, 1547, 1603, 1960
BshNI	GGYRCC	1 cut(s)	146
BsiHKCI	CYCGRG	1 cut(s)	1865
BsiSI	CCGG	2 cut(s)	1045, 1342
BslI	CCNNNNNNNGG	8 cut(s)	131, 248, 349, 789, 790, 1710, 1871, 2105
BsmAI	GTCTC	9 cut(s)	161, 401, 659, 666, 975, 1790, 1827, 1862, 2100
BsmBI	CGTCTC	1 cut(s)	401
BsnI	GGCC	5 cut(s)	183, 385, 1547, 1603, 1960
Bso31I	GGTCTC	3 cut(s)	666, 975, 2100
BsoBI	CYCGRG	1 cut(s)	1865
Bsp1286I	GDGCHC	1 cut(s)	2006
Bsp13I	TCCGGA	1 cut(s)	1044
Bsp143I	GATC	8 cut(s)	227, 339, 526, 873, 909, 994, 1309, 1583
BspACI	CCGC	5 cut(s)	11, 719, 746, 1360, 2091
BspANI	GGCC	5 cut(s)	183, 385, 1547, 1603, 1960
BspCNI	CTCAG	1 cut(s)	1329
BspEI	TCCGGA	1 cut(s)	1044
BspHI	TCATGA	1 cut(s)	462
BspLI	GGNNCC	4 cut(s)	148, 261, 528, 911
BspMAI	CTGCAG	3 cut(s)	155, 733, 1761
BspOI	GCTAGC	1 cut(s)	555
BspPI	GGATC	8 cut(s)	334, 521, 534, 868, 904, 917, 1317, 1591
BspT107I	GGYRCC	1 cut(s)	146
BspTNI	GGTCTC	3 cut(s)	666, 975, 2100
BsrDI	GCAATG	2 cut(s)	217, 357
BsrI	ACTGG	3 cut(s)	194, 502, 1876
BssECI	CCNNGG	3 cut(s)	1578, 1787, 1984
BssMI	GATC	8 cut(s)	227, 339, 526, 873, 909, 994, 1309, 1583
BssT1I	CCWWGG	2 cut(s)	1578, 1984
Bst2UI	CCWGG	3 cut(s)	243, 557, 1788
Bst4CI	ACNGT	6 cut(s)	604, 817, 949, 1033, 1366, 1382
Bst6I	CTCTTC	2 cut(s)	1186, 1965
BstAPI	GCANNNNNTGC	1 cut(s)	1183
BstBAI	YACGTR	2 cut(s)	179, 314
BstC8I	GCNNGC	4 cut(s)	151, 553, 1347, 1605
BstDEI	CTNAG	5 cut(s)	918, 1052, 1316, 1674, 1842
BstF5I	GGATG	6 cut(s)	65, 444, 1111, 1356, 2119, 2128
BstHHI	GCGC	1 cut(s)	1143
BstKTI	GATC	8 cut(s)	230, 342, 529, 876, 912, 997, 1312, 1586
BstMAI	GTCTC	9 cut(s)	161, 401, 659, 666, 975, 1790, 1827, 1862, 2100
BstMBI	GATC	8 cut(s)	227, 339, 526, 873, 909, 994, 1309, 1583
BstMWI	GCNNNNNNNGC	8 cut(s)	359, 391, 557, 716, 1183, 1346, 1559, 1604
BstNI	CCWGG	3 cut(s)	243, 557, 1788
BstNSI	RCATGY	2 cut(s)	959, 1619
BstSCI	CCNGG	4 cut(s)	241, 555, 1340, 1786
BstSFI	CTRYAG	5 cut(s)	151, 531, 729, 1125, 1757
BstV1I	GCAGC	8 cut(s)	730, 1186, 1451, 1571, 1652, 1743, 1952, 2088
BstV2I	GAAGAC	2 cut(s)	192, 208
BstX2I	RGATCY	4 cut(s)	227, 526, 909, 1309
BstYI	RGATCY	4 cut(s)	227, 526, 909, 1309
BsuRI	GGCC	5 cut(s)	183, 385, 1547, 1603, 1960
BtgZI	GCGATG	1 cut(s)	1005
BtsCI	GGATG	6 cut(s)	65, 444, 1111, 1356, 2119, 2128
BtsI	GCAGTG	2 cut(s)	178, 354
BtsIMutI	CAGTG	7 cut(s)	178, 201, 354, 609, 1029, 1387, 2077
Cac8I	GCNNGC	4 cut(s)	151, 553, 1347, 1605
CaiI	CAGNNNCTG	1 cut(s)	1805
CciI	TCATGA	1 cut(s)	462
CfoI	GCGC	1 cut(s)	1143
Cfr13I	GGNCC	7 cut(s)	182, 191, 239, 297, 383, 1546, 1601
CseI	GACGC	2 cut(s)	383, 1276
Csp6I	GTAC	4 cut(s)	176, 945, 1527, 1665
CspCI	CAANNNNNGTGG	2 cut(s)	37, 72
CviAII	CATG	9 cut(s)	8, 131, 463, 760, 878, 956, 1337, 1616, 1853
CviQI	GTAC	4 cut(s)	176, 945, 1527, 1665
DdeI	CTNAG	5 cut(s)	918, 1052, 1316, 1674, 1842
DpnI	GATC	8 cut(s)	229, 341, 528, 875, 911, 996, 1311, 1585
DpnII	GATC	8 cut(s)	227, 339, 526, 873, 909, 994, 1309, 1583
Eam1104I	CTCTTC	2 cut(s)	1186, 1965
EarI	CTCTTC	2 cut(s)	1186, 1965
Eco130I	CCWWGG	2 cut(s)	1578, 1984
Eco147I	AGGCCT	1 cut(s)	1960
Eco24I	GRGCYC	1 cut(s)	2006
Eco31I	GGTCTC	3 cut(s)	666, 975, 2100
Eco47I	GGWCC	3 cut(s)	191, 239, 297
Eco57I	CTGAAG	3 cut(s)	461, 528, 1575
Eco88I	CYCGRG	1 cut(s)	1865
EcoO109I	RGGNCCY	1 cut(s)	297
EcoRI	GAATTC	1 cut(s)	2066
EcoRII	CCWGG	3 cut(s)	241, 555, 1786
EcoT14I	CCWWGG	2 cut(s)	1578, 1984
EcoT38I	GRGCYC	1 cut(s)	2006
ErhI	CCWWGG	2 cut(s)	1578, 1984
Esp3I	CGTCTC	1 cut(s)	401
FaeI	CATG	9 cut(s)	11, 134, 466, 763, 881, 959, 1340, 1619, 1856
FalI	AAGNNNNNCTT	2 cut(s)	310, 342
FatI	CATG	9 cut(s)	7, 130, 462, 759, 877, 955, 1336, 1615, 1852
FauI	CCCGC	1 cut(s)	2084
FauNDI	CATATG	1 cut(s)	105
FokI	GGATG	5 cut(s)	72, 451, 1118, 1343, 2126
FriOI	GRGCYC	1 cut(s)	2006
FspBI	CTAG	3 cut(s)	552, 1434, 1956
FspI	TGCGCA	1 cut(s)	1142
GlaI	GCGC	1 cut(s)	1142
GsaI	CCCAGC	4 cut(s)	347, 773, 804, 1602
GsuI	CTGGAG	1 cut(s)	519
HaeIII	GGCC	5 cut(s)	183, 385, 1547, 1603, 1960
HapII	CCGG	2 cut(s)	1045, 1342
HgaI	GACGC	2 cut(s)	383, 1276
HhaI	GCGC	1 cut(s)	1143
Hin1II	CATG	9 cut(s)	11, 134, 466, 763, 881, 959, 1340, 1619, 1856
Hin6I	GCGC	1 cut(s)	1141
HinP1I	GCGC	1 cut(s)	1141
HincII	GTYRAC	1 cut(s)	901
HindII	GTYRAC	1 cut(s)	901
HindIII	AAGCTT	1 cut(s)	631
HinfI	GANTC	9 cut(s)	774, 1048, 1074, 1081, 1574, 1714, 1730, 1808, 2038
HpaI	GTTAAC	1 cut(s)	901
HpaII	CCGG	2 cut(s)	1045, 1342
HphI	GGTGA	5 cut(s)	1076, 1325, 1557, 1846, 1854
Hpy166II	GTNNAC	4 cut(s)	176, 901, 1009, 1029
Hpy188I	TCNGA	8 cut(s)	270, 547, 1319, 1573, 1588, 1684, 1726, 1750
Hpy8I	GTNNAC	4 cut(s)	176, 901, 1009, 1029
Hpy99I	CGWCG	1 cut(s)	423
HpyAV	CCTTC	6 cut(s)	485, 552, 920, 1786, 2109, 2113
HpyCH4III	ACNGT	6 cut(s)	604, 817, 949, 1033, 1366, 1382
HpyCH4IV	ACGT	3 cut(s)	178, 313, 571
HpyF10VI	GCNNNNNNNGC	8 cut(s)	359, 391, 557, 716, 1183, 1346, 1559, 1604
HpyF3I	CTNAG	5 cut(s)	918, 1052, 1316, 1674, 1842
HpySE526I	ACGT	3 cut(s)	178, 313, 571
Hsp92II	CATG	9 cut(s)	11, 134, 466, 763, 881, 959, 1340, 1619, 1856
HspAI	GCGC	1 cut(s)	1141
Kpn2I	TCCGGA	1 cut(s)	1044
KspAI	GTTAAC	1 cut(s)	901
Kzo9I	GATC	8 cut(s)	227, 339, 526, 873, 909, 994, 1309, 1583
LmnI	GCTCC	2 cut(s)	500, 1802
Lsp1109I	GCAGC	8 cut(s)	730, 1186, 1451, 1571, 1652, 1743, 1952, 2088
LweI	GCATC	5 cut(s)	50, 64, 123, 197, 1579
MaeI	CTAG	3 cut(s)	552, 1434, 1956
MaeII	ACGT	3 cut(s)	178, 313, 571
MaeIII	GTNAC	9 cut(s)	44, 126, 450, 680, 793, 1097, 1490, 1609, 1834
MalI	GATC	8 cut(s)	229, 341, 528, 875, 911, 996, 1311, 1585
MboI	GATC	8 cut(s)	227, 339, 526, 873, 909, 994, 1309, 1583
MboII	GAAGA	7 cut(s)	113, 192, 208, 407, 1203, 1304, 1982
MflI	RGATCY	4 cut(s)	227, 526, 909, 1309
MhlI	GDGCHC	1 cut(s)	2006
MluCI	AATT	8 cut(s)	335, 580, 636, 703, 764, 978, 1116, 2066
MlyI	GAGTC	3 cut(s)	1075, 1568, 2032
MmeI	TCCRAC	2 cut(s)	998, 1680
MroI	TCCGGA	1 cut(s)	1044
MroXI	GAANNNNTTC	1 cut(s)	1005
MseI	TTAA	7 cut(s)	167, 333, 635, 900, 1119, 1212, 1257
MslI	CAYNNNNRTG	4 cut(s)	511, 1104, 1335, 2114
MspI	CCGG	2 cut(s)	1045, 1342
MspR9I	CCNGG	4 cut(s)	243, 557, 1342, 1788
MvaI	CCWGG	3 cut(s)	243, 557, 1788
MwoI	GCNNNNNNNGC	8 cut(s)	359, 391, 557, 716, 1183, 1346, 1559, 1604
NciI	CCSGG	1 cut(s)	1342
NdeI	CATATG	1 cut(s)	105
NdeII	GATC	8 cut(s)	227, 339, 526, 873, 909, 994, 1309, 1583
NheI	GCTAGC	1 cut(s)	551
NlaIII	CATG	9 cut(s)	11, 134, 466, 763, 881, 959, 1340, 1619, 1856
NlaIV	GGNNCC	4 cut(s)	148, 261, 528, 911
NmeAIII	GCCGAG	1 cut(s)	378
NmuCI	GTSAC	7 cut(s)	44, 126, 450, 680, 1097, 1490, 1834
NsbI	TGCGCA	1 cut(s)	1142
NspI	RCATGY	2 cut(s)	959, 1619
OliI	CACNNNNGTG	1 cut(s)	2114
PagI	TCATGA	1 cut(s)	462
PceI	AGGCCT	1 cut(s)	1960
PcsI	WCGNNNNNNNCGW	2 cut(s)	61, 1522
PdmI	GAANNNNTTC	1 cut(s)	1005
PfeI	GAWTC	6 cut(s)	774, 1048, 1074, 1714, 1730, 1808
PflFI	GACNNNGTC	1 cut(s)	295
PleI	GAGTC	3 cut(s)	1075, 1568, 2032
PpsI	GAGTC	3 cut(s)	1075, 1568, 2032
Ppu21I	YACGTR	2 cut(s)	179, 314
PpuMI	RGGWCCY	1 cut(s)	297
PshBI	ATTAAT	2 cut(s)	1119, 1212
PsiI	TTATAA	2 cut(s)	578, 983
Psp5II	RGGWCCY	1 cut(s)	297
Psp6I	CCWGG	3 cut(s)	241, 555, 1786
PspFI	CCCAGC	4 cut(s)	343, 769, 800, 1598
PspGI	CCWGG	3 cut(s)	241, 555, 1786
PspN4I	GGNNCC	4 cut(s)	148, 261, 528, 911
PspPI	GGNCC	7 cut(s)	182, 191, 239, 297, 383, 1546, 1601
PspPPI	RGGWCCY	1 cut(s)	297
PstI	CTGCAG	3 cut(s)	155, 733, 1761
PstNI	CAGNNNCTG	1 cut(s)	1805
PsuI	RGATCY	4 cut(s)	227, 526, 909, 1309
PsyI	GACNNNGTC	1 cut(s)	295
RsaI	GTAC	4 cut(s)	177, 946, 1528, 1666
RsaNI	GTAC	4 cut(s)	176, 945, 1527, 1665
RseI	CAYNNNNRTG	4 cut(s)	511, 1104, 1335, 2114
SaqAI	TTAA	7 cut(s)	167, 333, 635, 900, 1119, 1212, 1257
Sau3AI	GATC	8 cut(s)	227, 339, 526, 873, 909, 994, 1309, 1583
Sau96I	GGNCC	7 cut(s)	182, 191, 239, 297, 383, 1546, 1601
SchI	GAGTC	3 cut(s)	1075, 1568, 2032
ScrFI	CCNGG	4 cut(s)	243, 557, 1342, 1788
SduI	GDGCHC	1 cut(s)	2006
SfaNI	GCATC	5 cut(s)	50, 64, 123, 197, 1579
SfcI	CTRYAG	5 cut(s)	151, 531, 729, 1125, 1757
SinI	GGWCC	3 cut(s)	191, 239, 297
SmiMI	CAYNNNNRTG	4 cut(s)	511, 1104, 1335, 2114
SmlI	CTYRAG	2 cut(s)	1164, 1660
SmoI	CTYRAG	2 cut(s)	1164, 1660
Sse9I	AATT	8 cut(s)	335, 580, 636, 703, 764, 978, 1116, 2066
SseBI	AGGCCT	1 cut(s)	1960
SsiI	CCGC	5 cut(s)	11, 719, 746, 1360, 2091
SspI	AATATT	3 cut(s)	1210, 1376, 1978
SspMI	CTAG	3 cut(s)	552, 1434, 1956
StuI	AGGCCT	1 cut(s)	1960
StyD4I	CCNGG	4 cut(s)	241, 555, 1340, 1786
StyI	CCWWGG	2 cut(s)	1578, 1984
TaaI	ACNGT	6 cut(s)	604, 817, 949, 1033, 1366, 1382
TaiI	ACGT	3 cut(s)	181, 316, 574
TaqI	TCGA	4 cut(s)	97, 418, 1949, 2025
TasI	AATT	8 cut(s)	335, 580, 636, 703, 764, 978, 1116, 2066
TatI	WGTACW	1 cut(s)	1664
TauI	GCSGC	1 cut(s)	14
TfiI	GAWTC	6 cut(s)	774, 1048, 1074, 1714, 1730, 1808
Tru1I	TTAA	7 cut(s)	167, 333, 635, 900, 1119, 1212, 1257
Tru9I	TTAA	7 cut(s)	167, 333, 635, 900, 1119, 1212, 1257
TscAI	CASTG	7 cut(s)	178, 201, 354, 609, 1036, 1387, 2077
TseFI	GTSAC	7 cut(s)	44, 126, 450, 680, 1097, 1490, 1834
TseI	GCWGC	9 cut(s)	14, 743, 1174, 1439, 1559, 1640, 1756, 1940, 2076
Tsp45I	GTSAC	7 cut(s)	44, 126, 450, 680, 1097, 1490, 1834
TspDTI	ATGAA	3 cut(s)	455, 748, 948
TspGWI	ACGGA	5 cut(s)	114, 436, 1443, 2092, 2126
TspRI	CASTG	7 cut(s)	178, 201, 354, 609, 1036, 1387, 2077
Tth111I	GACNNNGTC	1 cut(s)	295
VpaK11BI	GGWCC	3 cut(s)	191, 239, 297
VspI	ATTAAT	2 cut(s)	1119, 1212
XapI	RAATTY	2 cut(s)	580, 2066
XceI	RCATGY	2 cut(s)	959, 1619
XmnI	GAANNNNTTC	1 cut(s)	1005
XspI	CTAG	3 cut(s)	552, 1434, 1956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.