FvH4_5g35830

WD and tetratricopeptide repeats protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007919
Physical Location & Seq
Forward (+)
26242667 .. 26253124
10458 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35830.t2

Sequence Viewer

Length: 2289 bp
ATGGAGGCCTTGCCTTTTCACGACGGCAACATCCACGACTTGATCGAGAGCCGGTACCTCGACGTCCGTCAAGATGTTAATCACAGCTTGCAGATGCACTCGTCTTTGGTACGGCGGCTTTCGCAGGAGACGGAATTGGAGGGGCATCAGGGTTGTGTTAATTCGATTGCTTGGAATTCTAGAGGTTCACTTTTGATATCTGGATCAGACGATACACGGATAAATATCTGGAGCTATTCTGGTCGGAAGCTTTTGCATTCTATTGAGACTGGGCACTGCGCAAACATATTTTGTACAAAATTTATTCCTGAAACTTCTGATGAGCTTGTCGTCTCTGGTGCTGGGGATGCAGAAGTTCGGTTATTTAATTTGTCTCAGTTAAGTGGGAGAGGACCTGAAGATAATGCTGTTCCTCCATCAGCTCTGTATCAATGTCACTCAAGAAGAGTCAAGAAGTTAGCTGTTGAAGTTGGGAATCCCAATGTGGTATGGAGTGCAAGTGAAGATGGAACTTTGAGACAGCATGACTTTCGAGAGGGCACTTCTTGCCCCCCTGCTGGATCTGCTCATCAAGAATGTCGTAATGTTTTACTTGACTTGCGGTCTGGAGCAAAGAAGTCACTAGCTGATCCTCCGAAACAAACGTTTGCTCTAAAATCTTGTGATATCAGTTCCACTAGGCCTCATTTGCTTTTAGTTGGTGGGAGTGATGCATTTGCTCGTTTATATGATAGAAGGATGCTTCCTCCTCTGACATCCTGCCGGAAAAGGACGTCTCCACCTCCTTGTGTTAACTATTTCTGTCCAATGCATCTCTCTGATCGTGGACGTACAAGCTTGCACTTAACTCATGTTGCATTTAGCCCGGATGGAGAAGAGGTTCTACTTAGTTATAGTGGGGAGCATGTATATTTGATGAACGTAAATAATGCCGGTGGAAGTGCTGTCCAGTATACTTCAGGAGATGCTTCAAAGATAATGAGCTTCACACCTATACTGAATGGGCTAGAACTGCAGCAACCAGCATCCAGAGTCTCCACAAGTCGTCTACCTAAAAGACGCAAGGCCACTGCTATGCATGAAAAGTGCAGTAAACTAACTCAAATTGCTCTGAAGTCCTTGGAGGAGGGGGATTTTTATCATGGCATTGAGGCATGCAATGAGGTGCTGGATGGATATGGTCGCGATATTGGGCCCATACTAAGGCATGATTGTTTCTGTACTCGTGCCGCATTATTGCTGAAGCGTAATTGGAAAAATGATGCTCATATGGCTATAAGGGACTGCTATAATGCCCGGAAAATCGATAGCTCCTCTTTCAGAGCTCATTGCCATATGTCTAAAGCTTTATCACAGTTGGGCAAACATAAAGAAGCTCTAGAATTCGCTATGGCAGCCCAATCTTTAGCTCCATCAAATCCTGAGGTAGCAGCACAGTTGGAGAGTATAAAAAGAGAGCTTGCTGCAGCCGTGTCTGAAAAAAATGGTAAGGCAAGTGATGGAGCACCCAGGTCTGAACCTCGGGGTGGAAGAGTACTATCACTTAGTGACATACTTTACCGATCCGAGGGTAACAGTGAAGCTTCACAAGATGGGCCCAGATCTGAAAGAGAGGATTCTGATTACGATGAGGAATTGGAGTTGGACTTTGAAACGTCAATCTCAGGTGATGAAGAGCAAGATATCGAGCCCAACATCCTACATGGAAGTGTAAATCTGAGAATTCATCGAAGAAGTGATTCTTCTAGAGAAGTTGAAGGGGCAAATGGCTGTTGTGGATCACTGTCGTCATCATCTCAAAATGAAAGACTCCAATATCAGCCCGAGGCAGTTATTGATATGAAACAGAGATATGTCGGTCATTGTAACGTTGGAACTGACATAAAACAAGCGAGCTTTCTTGGCCAGAGAGGTGAATATATCGCTAGTGGAAGTGATGATGGCAGATGGTTTATATGGGAGAAACGAACTGGCAGACTGATAAAAATGCTTCAAGGAGATGAAGCTGTTGTGAACTGTGTACAGTGTCATCCCTCTGATTGTGTCGTGGCAACTAGTGGGATTGACAATACAATAAAGTTATGGACCCCAAGTGCTTCAGTCCCATCTATTGTAGCTGGTGGAGCCGCTGGACCAGAAACTGCTAATGTTTCTAATGTTTTGGCTGCTAATCAGCACAGATTGAGCCATAATCGTGAAACTATCCTCCATTCTGAAATTTTGGAGCATTTTCGAATGCATGAGTTCACTGAAGGAAGCTTGCACCCGTTTGAGTGTGCTCAGAGCTAA

Protein Analysis

763

Amino Acids

83.9

Weight (kDa)

6.11

Isoelectric Point (pI)

57.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 29 - 125 1.2e-09 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 32 - 126 7.5e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 38 - 181 1.6e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 41 - 126 8.4e-13 WDR3 first beta-propeller domain
WDR55 PF24796 42 - 180 8.2e-06 WDR55
WD40 PF00400 43 - 78 1.6e-08 WD domain, G-beta repeat
EIF3I PF24805 46 - 125 4.7e-06 EIF3I
Beta-prop_THOC3 PF25174 49 - 126 1.4e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 606 - 688 4.6e-13 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 608 - 691 4.3e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 621 - 692 2.2e-07 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 628 - 688 1.6e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 629 - 688 3.6e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 66, 776
Acc16I TGCGCA 1 cut(s) 280
Acc65I GGTACC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 54
AccI GTMKAC 2 cut(s) 953, 1048
AccII CGCG 1 cut(s) 1185
AciI CCGC 4 cut(s) 115, 601, 1230, 2127
AclI AACGTT 2 cut(s) 644, 1869
AclWI GGATC 5 cut(s) 211, 568, 623, 1557, 1786
AcoI YGGCCR 1 cut(s) 1903
AcsI RAATTY 5 cut(s) 175, 299, 1382, 1722, 2217
AcuI CTGAAG 6 cut(s) 417, 942, 1133, 1262, 2082, 2271
AcyI GRCGYC 2 cut(s) 63, 773
AdeI CACNNNGTG 1 cut(s) 1547
AfaI GTAC 7 cut(s) 56, 111, 295, 832, 1222, 1536, 2022
AfiI CCNNNNNNNGG 4 cut(s) 557, 1203, 1526, 1567
AgsI TTSAA 5 cut(s) 467, 972, 1652, 1757, 1994
AhdI GACNNNNNGTC 1 cut(s) 601
AhlI ACTAGT 1 cut(s) 2054
AjnI CCWGG 1 cut(s) 1508
AloI GAACNNNNNNTCC 2 cut(s) 864, 896
Alw21I GWGCWC 3 cut(s) 1327, 1507, 2281
Alw26I GTCTC 7 cut(s) 122, 260, 337, 378, 511, 780, 1039
AlwI GGATC 5 cut(s) 211, 568, 623, 1557, 1786
AlwNI CAGNNNCTG 1 cut(s) 2141
Ama87I CYCGRG 2 cut(s) 1521, 1823
AoxI GGCC 6 cut(s) 6, 680, 1065, 1193, 1595, 1903
ApaI GGGCCC 2 cut(s) 1197, 1599
ApeKI GCWGC 6 cut(s) 1015, 1394, 1430, 1463, 1466, 2165
ApoI RAATTY 5 cut(s) 175, 299, 1382, 1722, 2217
Asp700I GAANNNNTTC 2 cut(s) 879, 1738
Asp718I GGTACC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 7 cut(s) 392, 1193, 1194, 1595, 1596, 2085, 2132
AsuC2I CCSGG 2 cut(s) 866, 1297
AsuHPI GGTGA 2 cut(s) 1679, 1925
AsuII TTCGAA 1 cut(s) 2233
AvaI CYCGRG 2 cut(s) 1521, 1823
AvaII GGWCC 3 cut(s) 392, 2085, 2132
AxyI CCTNAGG 1 cut(s) 1422
BaeGI GKGCMC 4 cut(s) 276, 542, 1197, 1599
BalI TGGCCA 1 cut(s) 1905
BanI GGYRCC 1 cut(s) 54
BanII GRGCYC 4 cut(s) 1197, 1327, 1599, 1692
BarI GAAGNNNNNNTAC 2 cut(s) 867, 899
BauI CACGAG 1 cut(s) 1224
Bbv12I GWGCWC 3 cut(s) 1327, 1507, 2281
BbvI GCAGC 6 cut(s) 1027, 1406, 1442, 1450, 1478, 2152
BceAI ACGGC 3 cut(s) 40, 128, 1454
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BciT130I CCWGG 1 cut(s) 1510
BcnI CCSGG 2 cut(s) 866, 1297
BcoDI GTCTC 7 cut(s) 122, 260, 337, 378, 511, 780, 1039
BcuI ACTAGT 1 cut(s) 2054
BfaI CTAG 8 cut(s) 180, 623, 678, 1007, 1379, 1746, 1926, 2055
BfmI CTRYAG 2 cut(s) 1013, 1464
BglII AGATCT 1 cut(s) 1601
BisI GCNGC 9 cut(s) 116, 1016, 1230, 1395, 1431, 1464, 1467, 2127, 2166
BlsI GCNGC 9 cut(s) 117, 1017, 1231, 1396, 1432, 1465, 1468, 2128, 2167
BmcAI AGTACT 1 cut(s) 1536
Bme1390I CCNGG 3 cut(s) 866, 1297, 1510
Bme18I GGWCC 3 cut(s) 392, 2085, 2132
BmeRI GACNNNNNGTC 1 cut(s) 601
BmeT110I CYCGRG 2 cut(s) 1521, 1823
BmgT120I GGNCC 7 cut(s) 392, 1193, 1194, 1595, 1596, 2085, 2132
BmiI GGNNCC 5 cut(s) 56, 1195, 1597, 2087, 2125
BmrFI CCNGG 3 cut(s) 866, 1297, 1510
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 9 cut(s) 84, 154, 337, 700, 729, 820, 955, 1034, 1252
BmuI ACTGGG 1 cut(s) 279
BoxI GACNNNNGTC 1 cut(s) 66
BpmI CTGGAG 2 cut(s) 250, 627
Bpu14I TTCGAA 1 cut(s) 2233
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 2 cut(s) 866, 1297
Bsa29I ATCGAT 1 cut(s) 1305
BsaBI GATNNNNATC 3 cut(s) 78, 224, 1137
BsaHI GRCGYC 2 cut(s) 63, 773
BsaJI CCNNGG 5 cut(s) 1119, 1508, 1520, 1566, 1824
BsaXI ACNNNNNCTCC 4 cut(s) 484, 514, 864, 894
Bsc4I CCNNNNNNNGG 4 cut(s) 557, 1203, 1526, 1567
Bse118I RCCGGY 2 cut(s) 51, 932
Bse1I ACTGG 3 cut(s) 274, 949, 1975
Bse21I CCTNAGG 1 cut(s) 1422
Bse3DI GCAATG 2 cut(s) 1165, 1327
Bse8I GATNNNNATC 3 cut(s) 78, 224, 1137
BseBI CCWGG 1 cut(s) 1510
BseCI ATCGAT 1 cut(s) 1305
BseDI CCNNGG 5 cut(s) 1119, 1508, 1520, 1566, 1824
BseGI GGATG 9 cut(s) 30, 352, 744, 755, 874, 1025, 1177, 1695, 2029
BseJI GATNNNNATC 3 cut(s) 78, 224, 1137
BseLI CCNNNNNNNGG 4 cut(s) 557, 1203, 1526, 1567
BseMI GCAATG 2 cut(s) 1165, 1327
BseMII CTCAG 4 cut(s) 389, 1413, 1677, 1709
BseNI ACTGG 3 cut(s) 274, 949, 1975
BseRI GAGGAG 3 cut(s) 738, 1139, 1303
BseSI GKGCMC 4 cut(s) 276, 542, 1197, 1599
BseXI GCAGC 6 cut(s) 1027, 1406, 1442, 1450, 1478, 2152
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 1108
Bsh1236I CGCG 1 cut(s) 1185
BshFI GGCC 6 cut(s) 8, 682, 1067, 1195, 1597, 1905
BshNI GGYRCC 1 cut(s) 54
BshVI ATCGAT 1 cut(s) 1305
BsiHKAI GWGCWC 3 cut(s) 1327, 1507, 2281
BsiHKCI CYCGRG 2 cut(s) 1521, 1823
BsiSI CCGG 5 cut(s) 52, 763, 866, 933, 1297
BslFI GGGAC 2 cut(s) 1295, 2087
BslI CCNNNNNNNGG 4 cut(s) 557, 1203, 1526, 1567
BsmAI GTCTC 7 cut(s) 122, 260, 337, 378, 511, 780, 1039
BsmBI CGTCTC 3 cut(s) 122, 337, 780
BsmFI GGGAC 2 cut(s) 1295, 2087
BsmI GAATGC 2 cut(s) 256, 2241
BsnI GGCC 6 cut(s) 8, 682, 1067, 1195, 1597, 1905
BsoBI CYCGRG 2 cut(s) 1521, 1823
Bsp119I TTCGAA 1 cut(s) 2233
Bsp120I GGGCCC 2 cut(s) 1193, 1595
Bsp1286I GDGCHC 8 cut(s) 276, 542, 1197, 1327, 1507, 1599, 1692, 2281
Bsp1407I TGTACA 2 cut(s) 293, 2020
Bsp143I GATC 8 cut(s) 42, 203, 560, 628, 820, 1562, 1601, 1778
Bsp68I TCGCGA 1 cut(s) 1185
BspACI CCGC 4 cut(s) 115, 601, 1230, 2127
BspANI GGCC 6 cut(s) 8, 682, 1067, 1195, 1597, 1905
BspCNI CTCAG 4 cut(s) 388, 1414, 1676, 1710
BspDI ATCGAT 1 cut(s) 1305
BspFNI CGCG 1 cut(s) 1185
BspLI GGNNCC 5 cut(s) 56, 1195, 1597, 2087, 2125
BspMAI CTGCAG 2 cut(s) 1017, 1468
BspPI GGATC 5 cut(s) 211, 568, 623, 1557, 1786
BspQI GCTCTTC 1 cut(s) 1668
BspT104I TTCGAA 1 cut(s) 2233
BspT107I GGYRCC 1 cut(s) 54
BsrDI GCAATG 2 cut(s) 1165, 1327
BsrFI RCCGGY 2 cut(s) 51, 932
BsrGI TGTACA 2 cut(s) 293, 2020
BsrI ACTGG 3 cut(s) 274, 949, 1975
BssAI RCCGGY 2 cut(s) 51, 932
BssECI CCNNGG 5 cut(s) 1119, 1508, 1520, 1566, 1824
BssMI GATC 8 cut(s) 42, 203, 560, 628, 820, 1562, 1601, 1778
BssNAI GTATAC 1 cut(s) 954
BssNI GRCGYC 2 cut(s) 63, 773
BssSI CACGAG 1 cut(s) 1224
BssT1I CCWWGG 1 cut(s) 1119
Bst1107I GTATAC 1 cut(s) 954
Bst2BI CACGAG 1 cut(s) 1224
Bst2UI CCWGG 1 cut(s) 1510
Bst4CI ACNGT 6 cut(s) 1356, 1437, 1577, 1785, 2018, 2025
Bst6I CTCTTC 4 cut(s) 439, 870, 1525, 1668
BstACI GRCGYC 2 cut(s) 63, 773
BstAPI GCANNNNNTGC 1 cut(s) 546
BstAUI TGTACA 2 cut(s) 293, 2020
BstBI TTCGAA 1 cut(s) 2233
BstC8I GCNNGC 6 cut(s) 89, 839, 1156, 1461, 1894, 2261
BstDEI CTNAG 8 cut(s) 375, 887, 1202, 1422, 1544, 1663, 1718, 2280
BstF5I GGATG 9 cut(s) 30, 352, 744, 755, 874, 1025, 1177, 1695, 2029
BstFNI CGCG 1 cut(s) 1185
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 8 cut(s) 45, 206, 563, 631, 823, 1565, 1604, 1781
BstMAI GTCTC 7 cut(s) 122, 260, 337, 378, 511, 780, 1039
BstMBI GATC 8 cut(s) 42, 203, 560, 628, 820, 1562, 1601, 1778
BstNI CCWGG 1 cut(s) 1510
BstNSI RCATGY 2 cut(s) 908, 1158
BstPAI GACNNNNGTC 1 cut(s) 66
BstSCI CCNGG 3 cut(s) 864, 1295, 1508
BstSFI CTRYAG 2 cut(s) 1013, 1464
BstSLI GKGCMC 4 cut(s) 276, 542, 1197, 1599
BstUI CGCG 1 cut(s) 1185
BstV1I GCAGC 6 cut(s) 1027, 1406, 1442, 1450, 1478, 2152
BstX2I RGATCY 2 cut(s) 560, 1601
BstYI RGATCY 2 cut(s) 560, 1601
BstZ17I GTATAC 1 cut(s) 954
Bsu15I ATCGAT 1 cut(s) 1305
Bsu36I CCTNAGG 1 cut(s) 1422
BsuRI GGCC 6 cut(s) 8, 682, 1067, 1195, 1597, 1905
BsuTUI ATCGAT 1 cut(s) 1305
BtsCI GGATG 9 cut(s) 30, 352, 744, 755, 874, 1025, 1177, 1695, 2029
BtsI GCAGTG 2 cut(s) 274, 1068
BtsIMutI CAGTG 6 cut(s) 274, 1068, 1582, 1781, 2030, 2247
BtuMI TCGCGA 1 cut(s) 1185
Cac8I GCNNGC 6 cut(s) 89, 839, 1156, 1461, 1894, 2261
CaiI CAGNNNCTG 1 cut(s) 2141
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 2 cut(s) 51, 932
Cfr13I GGNCC 7 cut(s) 392, 1193, 1194, 1595, 1596, 2085, 2132
ClaI ATCGAT 1 cut(s) 1305
CseI GACGC 1 cut(s) 1068
Csp6I GTAC 7 cut(s) 55, 110, 294, 831, 1221, 1535, 2021
CviAII CATG 9 cut(s) 524, 851, 905, 1079, 1142, 1155, 1208, 1703, 2240
CviQI GTAC 7 cut(s) 55, 110, 294, 831, 1221, 1535, 2021
DdeI CTNAG 8 cut(s) 375, 887, 1202, 1422, 1544, 1663, 1718, 2280
DpnI GATC 8 cut(s) 44, 205, 562, 630, 822, 1564, 1603, 1780
DpnII GATC 8 cut(s) 42, 203, 560, 628, 820, 1562, 1601, 1778
DraIII CACNNNGTG 1 cut(s) 1547
DriI GACNNNNNGTC 1 cut(s) 601
EaeI YGGCCR 1 cut(s) 1903
Eam1104I CTCTTC 4 cut(s) 439, 870, 1525, 1668
Eam1105I GACNNNNNGTC 1 cut(s) 601
EarI CTCTTC 4 cut(s) 439, 870, 1525, 1668
Ecl136II GAGCTC 1 cut(s) 1325
Eco130I CCWWGG 1 cut(s) 1119
Eco147I AGGCCT 2 cut(s) 8, 682
Eco24I GRGCYC 4 cut(s) 1197, 1327, 1599, 1692
Eco32I GATATC 3 cut(s) 198, 667, 1684
Eco47I GGWCC 3 cut(s) 392, 2085, 2132
Eco53kI GAGCTC 1 cut(s) 1325
Eco57I CTGAAG 6 cut(s) 417, 942, 1133, 1262, 2082, 2271
Eco81I CCTNAGG 1 cut(s) 1422
Eco88I CYCGRG 2 cut(s) 1521, 1823
EcoICRI GAGCTC 1 cut(s) 1325
EcoO109I RGGNCCY 1 cut(s) 392
EcoRI GAATTC 3 cut(s) 175, 1382, 1722
EcoRII CCWGG 1 cut(s) 1508
EcoRV GATATC 3 cut(s) 198, 667, 1684
EcoT14I CCWWGG 1 cut(s) 1119
EcoT22I ATGCAT 4 cut(s) 715, 813, 1080, 2241
EcoT38I GRGCYC 4 cut(s) 1197, 1327, 1599, 1692
ErhI CCWWGG 1 cut(s) 1119
Esp3I CGTCTC 3 cut(s) 122, 337, 780
FaeI CATG 9 cut(s) 527, 854, 908, 1082, 1145, 1158, 1211, 1706, 2243
FalI AAGNNNNNCTT 2 cut(s) 1726, 1758
FaqI GGGAC 2 cut(s) 1295, 2087
FatI CATG 9 cut(s) 523, 850, 904, 1078, 1141, 1154, 1207, 1702, 2239
FauNDI CATATG 2 cut(s) 1269, 1335
FblI GTMKAC 2 cut(s) 953, 1048
Fnu4HI GCNGC 9 cut(s) 116, 1016, 1230, 1395, 1431, 1464, 1467, 2127, 2166
FokI GGATG 9 cut(s) 17, 359, 742, 751, 881, 1012, 1184, 1682, 2016
FriOI GRGCYC 4 cut(s) 1197, 1327, 1599, 1692
Fsp4HI GCNGC 9 cut(s) 116, 1016, 1230, 1395, 1431, 1464, 1467, 2127, 2166
FspBI CTAG 8 cut(s) 180, 623, 678, 1007, 1379, 1746, 1926, 2055
FspI TGCGCA 1 cut(s) 280
GlaI GCGC 1 cut(s) 280
GluI GCNGC 9 cut(s) 116, 1016, 1230, 1395, 1431, 1464, 1467, 2127, 2166
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 2 cut(s) 250, 627
HaeIII GGCC 6 cut(s) 8, 682, 1067, 1195, 1597, 1905
HapII CCGG 5 cut(s) 52, 763, 866, 933, 1297
HgaI GACGC 1 cut(s) 1068
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 2 cut(s) 63, 773
Hin1II CATG 9 cut(s) 527, 854, 908, 1082, 1145, 1158, 1211, 1706, 2243
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HindIII AAGCTT 5 cut(s) 248, 835, 1344, 1581, 2257
HinfI GANTC 6 cut(s) 447, 475, 1032, 1616, 1739, 1809
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 5 cut(s) 52, 763, 866, 933, 1297
HphI GGTGA 2 cut(s) 1679, 1925
Hpy166II GTNNAC 9 cut(s) 188, 793, 827, 954, 1049, 1094, 2014, 2021, 2247
Hpy8I GTNNAC 9 cut(s) 188, 793, 827, 954, 1049, 1094, 2014, 2021, 2247
Hpy99I CGWCG 2 cut(s) 26, 65
HpyAV CCTTC 3 cut(s) 729, 1751, 2246
HpyCH4III ACNGT 6 cut(s) 1356, 1437, 1577, 1785, 2018, 2025
HpyCH4IV ACGT 7 cut(s) 63, 644, 773, 829, 921, 1655, 1869
HpyF3I CTNAG 8 cut(s) 375, 887, 1202, 1422, 1544, 1663, 1718, 2280
HpySE526I ACGT 7 cut(s) 63, 644, 773, 829, 921, 1655, 1869
Hsp92I GRCGYC 2 cut(s) 63, 773
Hsp92II CATG 9 cut(s) 527, 854, 908, 1082, 1145, 1158, 1211, 1706, 2243
HspAI GCGC 1 cut(s) 279
KpnI GGTACC 1 cut(s) 58
KspAI GTTAAC 1 cut(s) 793
Kzo9I GATC 8 cut(s) 42, 203, 560, 628, 820, 1562, 1601, 1778
LguI GCTCTTC 1 cut(s) 1668
LmnI GCTCC 8 cut(s) 231, 608, 901, 1316, 1414, 1502, 2123, 2224
Lsp1109I GCAGC 6 cut(s) 1027, 1406, 1442, 1450, 1478, 2152
LweI GCATC 9 cut(s) 84, 154, 337, 700, 729, 820, 955, 1034, 1252
MaeI CTAG 8 cut(s) 180, 623, 678, 1007, 1379, 1746, 1926, 2055
MaeII ACGT 7 cut(s) 63, 644, 773, 829, 921, 1655, 1869
MaeIII GTNAC 5 cut(s) 434, 618, 1547, 1571, 1865
MalI GATC 8 cut(s) 44, 205, 562, 630, 822, 1564, 1603, 1780
MboI GATC 8 cut(s) 42, 203, 560, 628, 820, 1562, 1601, 1778
MboII GAAGA 8 cut(s) 410, 456, 515, 887, 1542, 1685, 1734, 1743
MflI RGATCY 2 cut(s) 560, 1601
MhlI GDGCHC 8 cut(s) 276, 542, 1197, 1327, 1507, 1599, 1692, 2281
MlsI TGGCCA 1 cut(s) 1905
MluNI TGGCCA 1 cut(s) 1905
MlyI GAGTC 3 cut(s) 456, 1041, 1803
MmeI TCCRAC 4 cut(s) 224, 1419, 1623, 1852
Mox20I TGGCCA 1 cut(s) 1905
Mph1103I ATGCAT 4 cut(s) 715, 813, 1080, 2241
MroXI GAANNNNTTC 2 cut(s) 879, 1738
MscI TGGCCA 1 cut(s) 1905
MseI TTAA 6 cut(s) 78, 159, 366, 380, 792, 845
MslI CAYNNNNRTG 3 cut(s) 1073, 1707, 2193
Msp20I TGGCCA 1 cut(s) 1905
MspA1I CMGCKG 1 cut(s) 2129
MspI CCGG 5 cut(s) 52, 763, 866, 933, 1297
MspR9I CCNGG 3 cut(s) 866, 1297, 1510
Mva1269I GAATGC 2 cut(s) 256, 2241
MvaI CCWGG 1 cut(s) 1510
MvnI CGCG 1 cut(s) 1185
NciI CCSGG 2 cut(s) 866, 1297
NdeI CATATG 2 cut(s) 1269, 1335
NdeII GATC 8 cut(s) 42, 203, 560, 628, 820, 1562, 1601, 1778
NlaIII CATG 9 cut(s) 527, 854, 908, 1082, 1145, 1158, 1211, 1706, 2243
NlaIV GGNNCC 5 cut(s) 56, 1195, 1597, 2087, 2125
NmuCI GTSAC 3 cut(s) 434, 618, 1547
NruI TCGCGA 1 cut(s) 1185
NsbI TGCGCA 1 cut(s) 280
NsiI ATGCAT 4 cut(s) 715, 813, 1080, 2241
NspI RCATGY 2 cut(s) 908, 1158
NspV TTCGAA 1 cut(s) 2233
PaeI GCATGC 1 cut(s) 1158
PceI AGGCCT 2 cut(s) 8, 682
PciSI GCTCTTC 1 cut(s) 1668
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PctI GAATGC 2 cut(s) 256, 2241
PdmI GAANNNNTTC 2 cut(s) 879, 1738
PfeI GAWTC 3 cut(s) 475, 1616, 1739
PkrI GCNGC 9 cut(s) 117, 1017, 1231, 1396, 1432, 1465, 1468, 2128, 2167
PleI GAGTC 3 cut(s) 455, 1040, 1803
PpsI GAGTC 3 cut(s) 455, 1040, 1803
PpuMI RGGWCCY 1 cut(s) 392
PshAI GACNNNNGTC 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 1327
Psp1406I AACGTT 2 cut(s) 644, 1869
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 1508
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 1 cut(s) 1508
PspN4I GGNNCC 5 cut(s) 56, 1195, 1597, 2087, 2125
PspOMI GGGCCC 2 cut(s) 1193, 1595
PspPI GGNCC 7 cut(s) 392, 1193, 1194, 1595, 1596, 2085, 2132
PspPPI RGGWCCY 1 cut(s) 392
PstI CTGCAG 2 cut(s) 1017, 1468
PstNI CAGNNNCTG 1 cut(s) 2141
PsuI RGATCY 2 cut(s) 560, 1601
RruI TCGCGA 1 cut(s) 1185
RsaI GTAC 7 cut(s) 56, 111, 295, 832, 1222, 1536, 2022
RsaNI GTAC 7 cut(s) 55, 110, 294, 831, 1221, 1535, 2021
RseI CAYNNNNRTG 3 cut(s) 1073, 1707, 2193
SacI GAGCTC 1 cut(s) 1327
SapI GCTCTTC 1 cut(s) 1668
SaqAI TTAA 6 cut(s) 78, 159, 366, 380, 792, 845
SatI GCNGC 9 cut(s) 116, 1016, 1230, 1395, 1431, 1464, 1467, 2127, 2166
Sau3AI GATC 8 cut(s) 42, 203, 560, 628, 820, 1562, 1601, 1778
Sau96I GGNCC 7 cut(s) 392, 1193, 1194, 1595, 1596, 2085, 2132
ScaI AGTACT 1 cut(s) 1536
SchI GAGTC 3 cut(s) 456, 1041, 1803
ScrFI CCNGG 3 cut(s) 866, 1297, 1510
SduI GDGCHC 8 cut(s) 276, 542, 1197, 1327, 1507, 1599, 1692, 2281
SfaNI GCATC 9 cut(s) 84, 154, 337, 700, 729, 820, 955, 1034, 1252
SfcI CTRYAG 2 cut(s) 1013, 1464
SfuI TTCGAA 1 cut(s) 2233
SinI GGWCC 3 cut(s) 392, 2085, 2132
SmiMI CAYNNNNRTG 3 cut(s) 1073, 1707, 2193
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
SpeI ACTAGT 1 cut(s) 2054
SphI GCATGC 1 cut(s) 1158
SseBI AGGCCT 2 cut(s) 8, 682
SsiI CCGC 4 cut(s) 115, 601, 1230, 2127
SspMI CTAG 8 cut(s) 180, 623, 678, 1007, 1379, 1746, 1926, 2055
SstI GAGCTC 1 cut(s) 1327
StuI AGGCCT 2 cut(s) 8, 682
StyD4I CCNGG 3 cut(s) 864, 1295, 1508
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 6 cut(s) 1356, 1437, 1577, 1785, 2018, 2025
TaiI ACGT 7 cut(s) 66, 647, 776, 832, 924, 1658, 1872
TaqI TCGA 8 cut(s) 45, 60, 164, 532, 1305, 1686, 1729, 2233
TaqII GACCGA 1 cut(s) 1847
TatI WGTACW 4 cut(s) 293, 1220, 1534, 2020
TauI GCSGC 3 cut(s) 118, 1232, 2129
TfiI GAWTC 3 cut(s) 475, 1616, 1739
Tru1I TTAA 6 cut(s) 78, 159, 366, 380, 792, 845
Tru9I TTAA 6 cut(s) 78, 159, 366, 380, 792, 845
TscAI CASTG 6 cut(s) 281, 1075, 1582, 1788, 2030, 2254
TseFI GTSAC 3 cut(s) 434, 618, 1547
TseI GCWGC 6 cut(s) 1015, 1394, 1430, 1463, 1466, 2165
Tsp45I GTSAC 3 cut(s) 434, 618, 1547
TspDTI ATGAA 7 cut(s) 932, 1095, 1686, 1715, 1818, 1856, 2016
TspGWI ACGGA 3 cut(s) 56, 146, 232
TspRI CASTG 6 cut(s) 281, 1075, 1582, 1788, 2030, 2254
VpaK11BI GGWCC 3 cut(s) 392, 2085, 2132
XapI RAATTY 5 cut(s) 175, 299, 1382, 1722, 2217
XbaI TCTAGA 3 cut(s) 179, 1378, 1745
XceI RCATGY 2 cut(s) 908, 1158
XmiI GTMKAC 2 cut(s) 953, 1048
XmnI GAANNNNTTC 2 cut(s) 879, 1738
XspI CTAG 8 cut(s) 180, 623, 678, 1007, 1379, 1746, 1926, 2055
ZraI GACGTC 2 cut(s) 64, 774
ZrmI AGTACT 1 cut(s) 1536
Zsp2I ATGCAT 4 cut(s) 715, 813, 1080, 2241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.