Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007919

Physical Location & Seq

Genomic Coordinates Reverse (-)

24823367 .. 24845327

Gene Structure 21961 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1192800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2289 bp

ATGGAAGGTGTGCCTTTTCACGACGGCAACATCCACGACTTGATTGAGAGACGATACGTCGACGTTCGGCACGATGCCAATCAAAGGTTGCAGATGCACTCGTCGTTGGTGCGAAGGCTTTCGCAGGAAAGGGAATTGGAGGGGCATCAAGGTTGTGTTAATTCTATTGCTTGGAATTCGAATGGCTCGCTTTTAATATCTGGATCAGATGACACACGGATAAATATCTGGAACTACCCTAGTCGGAAGCTTTTGCATTCTATAGAGACTGGGCATTGTGCGAACATATTTTGTACAAAATTTGTCCCTGAAACTTCTGATGAGCTCGTGGTCTCTGGAGCTGGAGATGCAGAAGTTCGATTATTTAATTTGTCTCGTTTAAATGGGAGAGGAACTGATGATAATGCTATTTCTCCATCAGCTGTGTATCAATGCCATACTAGAAGAGTAAAAAAGTTAGCTGTTGAAGTTGGAAATCCCAATGTGGTATGGAGTGCAAGTGAAGATGGAACTTTGAGACAGCATGACTTCCGAGAGGGTACTTCTTGCCCACTTGCTGGATCCACTAATCAAGAATGTCGCAATGTTTTGCTTGACTTGCGGTGTGGAGCAAAGAAGTCACTGGCTGATCCCCCCAAACAAAATTTTTCTTTAAAATCTTGTGATATCAGTTCCACTAGACCTCATTTACTCCTAGTTGGCGGGAGTGATGCATTTGCACGTTTATATGATAGAAGGATGCTGCCACCACTGACATCCTGTCGGAAACGGACAGCGCCACCTCCTTGTGTTAACTATTTTTGTCCAATGCATCTCTCTGACCGTGGACGTGCTAGCTTGCATTTGACTCATGTTGCATTTAGTCCAGATGGAGAAGAGGTTCTACTTAGTTATAGTGGGGAGCACGTATATTTGATGAATGTAAATCATGCTGGTGAGAGTGTTATGCAGTATACTCCTGGAGATGTTTCGAAACTAACGAGCTTCAGTCCTCTACTCAATGGTGCTGAACTGCATCAACCAGCATCCATTGTGTTCAGAAATGGTGTTCCTAAAAGAGGCAACATTGCTGCTAGGTTTGAGAAGTGCAGGAATCTAATTCAAATTGCTGAAAGATCCTTGGAGGGGACAGATTATTTTTATGGCATTGAAGCGTGCAATGAGGTGTTGGATGGAAACAGTTGTGGTATTGGGCCTACACTAAAGCATGATTGCCTTTGTATTCGTGCGGGATTGTTGCTTAAGCGCAAGTGGAAAAATGATGTTCATATGGCTATAAGAGATTGTTATAATGCTAGGAGGCTCAATAGCTCCTCTTTCAGTGCTCATTACTATATGTCTGATGCTTTATCGCAGTTGGCTAAACATAAAGAAGCTCTGGAATTTGCTATCGCAGCCCAATCTTTGGCTCCATCAAATTCTAAAGTAGCAGAACGATTGGAGCATGTAAAAAGGGATCTTGCTGCAGCTGAATCTGAAAGAAATGGTAAGCCAAATGATGGAGCACCCAGGTCTGAACCTCGAGGTGAAAGAGTACTATCACTGAGTGAACTACTCTACCGATCAGAGGGTAATAGTGATGTTTCACAAGATGGTCCAAGATCTGAAAGAGAAGATTCTGATTATGATGAGGAATTGGAGTTGGACTTTGAAACGTCGATCTCAGGTGATGAAGAGCATGACATCGAGCCCAATATTCTACAAGGAAGCTTAAACTTGAGAATTCATCGAAGAAGTGATTCTGCTAGAGAAGTTGGTGGTGCAAACGGCTCATCTGGATCCCCTTCTTTGTCGTCTCAAAATGAAAGACTAACATATCAGCCTGAGGCAGCGATTGATATGAAACAGAGATATGTTGGCCATTGTAATGTTGGAACTGACATAAAACAGGCCAGCTTTCTTGGGCAGCGAGGAGAATATGTTGCTAGTGGAAGTGATGATGGCAGATGGTTTGTCTGGGAGAAGCGAACTGGTAGACTGATAAAAATGTTTCAAGGAGATGAAGCTGTTGTGAACTGTATACAGTGCCATCCGTTTGATTGTGTTGTGGCAACTAGTGGAATTGACAACACAATAAAAATTTGGACGCCAAGTGCCTCAGTCCCATCTATTGTAGCTGGTGGAGCAGCAGGACCAGAAAATGCTGACGTTTCGGTAGTCATAGAATCCAATCAGCACAGATTAAGCCATAATCGCGAGACTATCCTCCGTTCTGAAATTTTGGAGCATTTTCGAATGCACGAGTTCGCTGAAGGAAGCTTGCACCCATTTGAGTGCGCTCAGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0019538 GO:0031974 GO:0031981 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

763

Amino Acids

84.41

Weight (kDa)

6.03

Isoelectric Point (pI)

52.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	27 - 125	2.7e-10	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	28 - 125	3.4e-07	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	38 - 181	1.7e-18	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	41 - 125	7.2e-13	WDR3 first beta-propeller domain
WDR55	PF24796	42 - 181	2.2e-06	WDR55
WD40	PF00400	43 - 78	1e-09	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	44 - 126	1e-11	THOC3 beta-propeller domain
EIF3I	PF24805	46 - 125	4.4e-06	EIF3I
Beta-prop_WDR5	PF25175	614 - 696	2.9e-12	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	616 - 699	1e-05	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	633 - 699	1.6e-06	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	637 - 696	7.2e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007919)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10940 AT5G10940
fragaria_vesca	FvH4_5g35830 FvH4_5g35830 FvH4_5g35830
malus_domestica	MD08G1192800.v1.1 MD15G1381500.v1.1
prunus_persica	Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1
pyrus_communis	pycom08g16630 pycom15g34170
rosa_chinensis	RchiOBHm_Chr7g0237521
rosa_laevigata	RLG00000000996
rosa_multiflora	Rmu_ssc0000152.1_g000001
rosa_roxburghii	Rroxscaffold_3G00224060
rosa_rugosa	Rorug07G0303800 Rorug07G0303900
rosa_samantha	Rh7AG461500 Rh7BG431700 Rh7CG478700 Rh7DG447900
rosa_wichuraiana	Rw7G038190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1290
AbsI	CCTCGAGG	1 cut(s)	1521
AccB7I	CCANNNNNTGG	3 cut(s)	557, 1405, 1499
AccI	GTMKAC	4 cut(s)	60, 953, 1975, 2020
AccII	CGCG	1 cut(s)	2196
AciI	CCGC	3 cut(s)	601, 702, 1229
AclWI	GGATC	8 cut(s)	211, 555, 568, 623, 1110, 1464, 1773, 1786
AcoI	YGGCCR	1 cut(s)	1858
AcsI	RAATTY	8 cut(s)	175, 299, 643, 1382, 1417, 1722, 2079, 2217
AcuI	CTGAAG	2 cut(s)	970, 2271
AcyI	GRCGYC	1 cut(s)	2087
AdeI	CACNNNGTG	1 cut(s)	1547
AfaI	GTAC	3 cut(s)	295, 541, 1536
AfiI	CCNNNNNNNGG	7 cut(s)	84, 557, 1058, 1125, 1405, 1499, 1567
AflII	CTTAAG	1 cut(s)	1241
AgsI	TTSAA	5 cut(s)	467, 1103, 1151, 1652, 1994
AhdI	GACNNNNNGTC	2 cut(s)	56, 759
AhlI	ACTAGT	1 cut(s)	2054
AjiI	CACGTC	1 cut(s)	830
AjnI	CCWGG	2 cut(s)	958, 1508
AloI	GAACNNNNNNTCC	2 cut(s)	864, 896
Alw21I	GWGCWC	4 cut(s)	327, 906, 1327, 1507
Alw26I	GTCTC	7 cut(s)	43, 260, 337, 378, 511, 1800, 2192
AlwI	GGATC	8 cut(s)	211, 555, 568, 623, 1110, 1464, 1773, 1786
Ama87I	CYCGRG	1 cut(s)	1521
AoxI	GGCC	3 cut(s)	1193, 1858, 1890
ApeKI	GCWGC	8 cut(s)	742, 1070, 1394, 1463, 1466, 1829, 1906, 2126
ApoI	RAATTY	8 cut(s)	175, 299, 643, 1382, 1417, 1722, 2079, 2217
Asp700I	GAANNNNTTC	3 cut(s)	118, 879, 1738
AspLEI	GCGC	3 cut(s)	778, 1248, 2279
AspS9I	GGNCC	3 cut(s)	1193, 1595, 2132
AsuHPI	GGTGA	3 cut(s)	947, 1538, 1679
AsuII	TTCGAA	3 cut(s)	179, 971, 2233
AsuNHI	GCTAGC	1 cut(s)	833
AvaI	CYCGRG	1 cut(s)	1521
AvaII	GGWCC	2 cut(s)	1595, 2132
AxyI	CCTNAGG	1 cut(s)	1824
BalI	TGGCCA	1 cut(s)	1860
BamHI	GGATCC	2 cut(s)	560, 1778
BanII	GRGCYC	2 cut(s)	327, 1692
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
BauI	CACGAG	2 cut(s)	326, 2240
Bbv12I	GWGCWC	4 cut(s)	327, 906, 1327, 1507
BbvI	GCAGC	8 cut(s)	729, 1057, 1406, 1450, 1478, 1841, 1918, 2138
BceAI	ACGGC	2 cut(s)	40, 1783
BciT130I	CCWGG	2 cut(s)	960, 1510
BcoDI	GTCTC	7 cut(s)	43, 260, 337, 378, 511, 1800, 2192
BcuI	ACTAGT	1 cut(s)	2054
BfmI	CTRYAG	2 cut(s)	261, 1464
BfoI	RGCGCY	1 cut(s)	779
BfrI	CTTAAG	1 cut(s)	1241
BglII	AGATCT	1 cut(s)	1601
BisI	GCNGC	8 cut(s)	743, 1071, 1395, 1464, 1467, 1830, 1907, 2127
BlsI	GCNGC	8 cut(s)	744, 1072, 1396, 1465, 1468, 1831, 1908, 2128
BmcAI	AGTACT	1 cut(s)	1536
Bme1390I	CCNGG	2 cut(s)	960, 1510
Bme18I	GGWCC	2 cut(s)	1595, 2132
BmeRI	GACNNNNNGTC	2 cut(s)	56, 759
BmeT110I	CYCGRG	1 cut(s)	1521
BmgBI	CACGTC	1 cut(s)	830
BmgT120I	GGNCC	3 cut(s)	1193, 1595, 2132
BmiI	GGNNCC	3 cut(s)	562, 1410, 1780
BmrFI	CCNGG	2 cut(s)	960, 1510
BmrI	ACTGGG	1 cut(s)	279
BmtI	GCTAGC	1 cut(s)	837
BmuI	ACTGGG	1 cut(s)	279
BpmI	CTGGAG	3 cut(s)	357, 363, 981
Bpu14I	TTCGAA	3 cut(s)	179, 971, 2233
BpuEI	CTTGAG	1 cut(s)	1738
BsaAI	YACGTR	1 cut(s)	907
BsaBI	GATNNNNATC	2 cut(s)	78, 224
BsaHI	GRCGYC	1 cut(s)	2087
BsaI	GGTCTC	1 cut(s)	337
BsaJI	CCNNGG	3 cut(s)	823, 1119, 1508
BsaXI	ACNNNNNCTCC	6 cut(s)	484, 514, 864, 894, 1905, 1935
Bsc4I	CCNNNNNNNGG	7 cut(s)	84, 557, 1058, 1125, 1405, 1499, 1567
Bse1I	ACTGG	3 cut(s)	274, 627, 1975
Bse21I	CCTNAGG	1 cut(s)	1824
Bse3DI	GCAATG	3 cut(s)	589, 1065, 1165
Bse8I	GATNNNNATC	2 cut(s)	78, 224
BseBI	CCWGG	2 cut(s)	960, 1510
BseDI	CCNNGG	3 cut(s)	823, 1119, 1508
BseGI	GGATG	6 cut(s)	30, 744, 755, 1025, 1177, 2029
BseJI	GATNNNNATC	2 cut(s)	78, 224
BseLI	CCNNNNNNNGG	7 cut(s)	84, 557, 1058, 1125, 1405, 1499, 1567
BseMI	GCAATG	3 cut(s)	589, 1065, 1165
BseMII	CTCAG	4 cut(s)	1535, 1677, 1815, 2112
BseNI	ACTGG	3 cut(s)	274, 627, 1975
BseRI	GAGGAG	2 cut(s)	1303, 1926
BseXI	GCAGC	8 cut(s)	729, 1057, 1406, 1450, 1478, 1841, 1918, 2138
BsgI	GTGCAG	1 cut(s)	1108
Bsh1236I	CGCG	1 cut(s)	2196
BshFI	GGCC	3 cut(s)	1195, 1860, 1892
BsiHKAI	GWGCWC	4 cut(s)	327, 906, 1327, 1507
BsiHKCI	CYCGRG	1 cut(s)	1521
BslFI	GGGAC	3 cut(s)	290, 1141, 2087
BslI	CCNNNNNNNGG	7 cut(s)	84, 557, 1058, 1125, 1405, 1499, 1567
BsmAI	GTCTC	7 cut(s)	43, 260, 337, 378, 511, 1800, 2192
BsmBI	CGTCTC	2 cut(s)	43, 1800
BsmFI	GGGAC	3 cut(s)	290, 1141, 2087
BsmI	GAATGC	2 cut(s)	256, 2241
BsnI	GGCC	3 cut(s)	1195, 1860, 1892
Bso31I	GGTCTC	1 cut(s)	337
BsoBI	CYCGRG	1 cut(s)	1521
Bsp119I	TTCGAA	3 cut(s)	179, 971, 2233
Bsp1286I	GDGCHC	5 cut(s)	327, 906, 1327, 1507, 1692
Bsp1407I	TGTACA	1 cut(s)	293
Bsp143I	GATC	9 cut(s)	203, 560, 628, 1115, 1456, 1562, 1601, 1659, 1778
Bsp68I	TCGCGA	1 cut(s)	2196
BspACI	CCGC	3 cut(s)	601, 702, 1229
BspANI	GGCC	3 cut(s)	1195, 1860, 1892
BspCNI	CTCAG	4 cut(s)	1536, 1676, 1816, 2111
BspFNI	CGCG	1 cut(s)	2196
BspLI	GGNNCC	3 cut(s)	562, 1410, 1780
BspMAI	CTGCAG	1 cut(s)	1468
BspOI	GCTAGC	1 cut(s)	837
BspPI	GGATC	8 cut(s)	211, 555, 568, 623, 1110, 1464, 1773, 1786
BspQI	GCTCTTC	1 cut(s)	1668
BspT104I	TTCGAA	3 cut(s)	179, 971, 2233
BspTI	CTTAAG	1 cut(s)	1241
BspTNI	GGTCTC	1 cut(s)	337
BsrDI	GCAATG	3 cut(s)	589, 1065, 1165
BsrGI	TGTACA	1 cut(s)	293
BsrI	ACTGG	3 cut(s)	274, 627, 1975
BssECI	CCNNGG	3 cut(s)	823, 1119, 1508
BssMI	GATC	9 cut(s)	203, 560, 628, 1115, 1456, 1562, 1601, 1659, 1778
BssNAI	GTATAC	2 cut(s)	954, 2021
BssNI	GRCGYC	1 cut(s)	2087
BssSI	CACGAG	2 cut(s)	326, 2240
BssT1I	CCWWGG	1 cut(s)	1119
Bst1107I	GTATAC	2 cut(s)	954, 2021
Bst2BI	CACGAG	2 cut(s)	326, 2240
Bst2UI	CCWGG	2 cut(s)	960, 1510
Bst4CI	ACNGT	4 cut(s)	824, 1181, 2018, 2025
Bst6I	CTCTTC	3 cut(s)	439, 870, 1668
BstACI	GRCGYC	1 cut(s)	2087
BstAFI	CTTAAG	1 cut(s)	1241
BstAUI	TGTACA	1 cut(s)	293
BstBAI	YACGTR	1 cut(s)	907
BstBI	TTCGAA	3 cut(s)	179, 971, 2233
BstC8I	GCNNGC	6 cut(s)	188, 835, 839, 1156, 1894, 2261
BstDEI	CTNAG	6 cut(s)	887, 1544, 1663, 1824, 2098, 2280
BstDSI	CCRYGG	1 cut(s)	823
BstENI	CCTNNNNNAGG	1 cut(s)	1056
BstF5I	GGATG	6 cut(s)	30, 744, 755, 1025, 1177, 2029
BstFNI	CGCG	1 cut(s)	2196
BstH2I	RGCGCY	1 cut(s)	779
BstHHI	GCGC	3 cut(s)	778, 1248, 2279
BstKTI	GATC	9 cut(s)	206, 563, 631, 1118, 1459, 1565, 1604, 1662, 1781
BstMAI	GTCTC	7 cut(s)	43, 260, 337, 378, 511, 1800, 2192
BstMBI	GATC	9 cut(s)	203, 560, 628, 1115, 1456, 1562, 1601, 1659, 1778
BstMWI	GCNNNNNNNGC	6 cut(s)	347, 598, 1394, 2123, 2193, 2283
BstNI	CCWGG	2 cut(s)	960, 1510
BstNSI	RCATGY	1 cut(s)	1448
BstSCI	CCNGG	2 cut(s)	958, 1508
BstSFI	CTRYAG	2 cut(s)	261, 1464
BstUI	CGCG	1 cut(s)	2196
BstV1I	GCAGC	8 cut(s)	729, 1057, 1406, 1450, 1478, 1841, 1918, 2138
BstX2I	RGATCY	5 cut(s)	560, 1115, 1456, 1601, 1778
BstYI	RGATCY	5 cut(s)	560, 1115, 1456, 1601, 1778
BstZ17I	GTATAC	2 cut(s)	954, 2021
Bsu36I	CCTNAGG	1 cut(s)	1824
BsuRI	GGCC	3 cut(s)	1195, 1860, 1892
BtgI	CCRYGG	1 cut(s)	823
BtrI	CACGTC	1 cut(s)	830
BtsCI	GGATG	6 cut(s)	30, 744, 755, 1025, 1177, 2029
BtsIMutI	CAGTG	5 cut(s)	620, 749, 1327, 1541, 2030
BtuMI	TCGCGA	1 cut(s)	2196
Cac8I	GCNNGC	6 cut(s)	188, 835, 839, 1156, 1894, 2261
CfoI	GCGC	3 cut(s)	778, 1248, 2279
Cfr13I	GGNCC	3 cut(s)	1193, 1595, 2132
CseI	GACGC	1 cut(s)	2095
Csp6I	GTAC	3 cut(s)	294, 540, 1535
CviAII	CATG	6 cut(s)	524, 851, 929, 1208, 1445, 1679
CviQI	GTAC	3 cut(s)	294, 540, 1535
DdeI	CTNAG	6 cut(s)	887, 1544, 1663, 1824, 2098, 2280
DpnI	GATC	9 cut(s)	205, 562, 630, 1117, 1458, 1564, 1603, 1661, 1780
DpnII	GATC	9 cut(s)	203, 560, 628, 1115, 1456, 1562, 1601, 1659, 1778
DraI	TTTAAA	2 cut(s)	381, 654
DraIII	CACNNNGTG	1 cut(s)	1547
DriI	GACNNNNNGTC	2 cut(s)	56, 759
EaeI	YGGCCR	1 cut(s)	1858
Eam1104I	CTCTTC	3 cut(s)	439, 870, 1668
Eam1105I	GACNNNNNGTC	2 cut(s)	56, 759
EarI	CTCTTC	3 cut(s)	439, 870, 1668
Ecl136II	GAGCTC	1 cut(s)	325
Eco130I	CCWWGG	1 cut(s)	1119
Eco24I	GRGCYC	2 cut(s)	327, 1692
Eco31I	GGTCTC	1 cut(s)	337
Eco32I	GATATC	1 cut(s)	667
Eco47I	GGWCC	2 cut(s)	1595, 2132
Eco53kI	GAGCTC	1 cut(s)	325
Eco57I	CTGAAG	2 cut(s)	970, 2271
Eco81I	CCTNAGG	1 cut(s)	1824
Eco88I	CYCGRG	1 cut(s)	1521
EcoICRI	GAGCTC	1 cut(s)	325
EcoNI	CCTNNNNNAGG	1 cut(s)	1056
EcoRI	GAATTC	2 cut(s)	175, 1722
EcoRII	CCWGG	2 cut(s)	958, 1508
EcoRV	GATATC	1 cut(s)	667
EcoT14I	CCWWGG	1 cut(s)	1119
EcoT22I	ATGCAT	2 cut(s)	715, 813
EcoT38I	GRGCYC	2 cut(s)	327, 1692
ErhI	CCWWGG	1 cut(s)	1119
Esp3I	CGTCTC	2 cut(s)	43, 1800
FaeI	CATG	6 cut(s)	527, 854, 932, 1211, 1448, 1682
FaqI	GGGAC	3 cut(s)	290, 1141, 2087
FatI	CATG	6 cut(s)	523, 850, 928, 1207, 1444, 1678
FauI	CCCGC	2 cut(s)	695, 1222
FauNDI	CATATG	1 cut(s)	1269
FblI	GTMKAC	4 cut(s)	60, 953, 1975, 2020
Fnu4HI	GCNGC	8 cut(s)	743, 1071, 1395, 1464, 1467, 1830, 1907, 2127
FokI	GGATG	6 cut(s)	17, 742, 751, 1012, 1184, 2016
FriOI	GRGCYC	2 cut(s)	327, 1692
Fsp4HI	GCNGC	8 cut(s)	743, 1071, 1395, 1464, 1467, 1830, 1907, 2127
GlaI	GCGC	3 cut(s)	777, 1247, 2278
GluI	GCNGC	8 cut(s)	743, 1071, 1395, 1464, 1467, 1830, 1907, 2127
GsuI	CTGGAG	3 cut(s)	357, 363, 981
HaeII	RGCGCY	1 cut(s)	779
HaeIII	GGCC	3 cut(s)	1195, 1860, 1892
HgaI	GACGC	1 cut(s)	2095
HhaI	GCGC	3 cut(s)	778, 1248, 2279
Hin1I	GRCGYC	1 cut(s)	2087
Hin1II	CATG	6 cut(s)	527, 854, 932, 1211, 1448, 1682
Hin6I	GCGC	3 cut(s)	776, 1246, 2277
HinP1I	GCGC	3 cut(s)	776, 1246, 2277
HincII	GTYRAC	2 cut(s)	61, 793
HindII	GTYRAC	2 cut(s)	61, 793
HindIII	AAGCTT	3 cut(s)	248, 1708, 2257
HinfI	GANTC	6 cut(s)	847, 1093, 1472, 1616, 1739, 2165
HpaI	GTTAAC	1 cut(s)	793
HphI	GGTGA	3 cut(s)	947, 1538, 1679
Hpy166II	GTNNAC	8 cut(s)	61, 793, 827, 954, 1550, 1976, 2014, 2021
Hpy188III	TCNNGA	9 cut(s)	20, 201, 229, 336, 572, 866, 1379, 1776, 2195
Hpy8I	GTNNAC	8 cut(s)	61, 793, 827, 954, 1550, 1976, 2014, 2021
Hpy99I	CGWCG	5 cut(s)	26, 62, 65, 106, 1660
HpyAV	CCTTC	4 cut(s)	108, 729, 1794, 2246
HpyCH4III	ACNGT	4 cut(s)	824, 1181, 2018, 2025
HpyCH4IV	ACGT	7 cut(s)	57, 63, 721, 829, 906, 1655, 2148
HpyF10VI	GCNNNNNNNGC	6 cut(s)	347, 598, 1394, 2123, 2193, 2283
HpyF3I	CTNAG	6 cut(s)	887, 1544, 1663, 1824, 2098, 2280
HpySE526I	ACGT	7 cut(s)	57, 63, 721, 829, 906, 1655, 2148
Hsp92I	GRCGYC	1 cut(s)	2087
Hsp92II	CATG	6 cut(s)	527, 854, 932, 1211, 1448, 1682
HspAI	GCGC	3 cut(s)	776, 1246, 2277
KspAI	GTTAAC	1 cut(s)	793
Kzo9I	GATC	9 cut(s)	203, 560, 628, 1115, 1456, 1562, 1601, 1659, 1778
LguI	GCTCTTC	1 cut(s)	1668
LmnI	GCTCC	9 cut(s)	338, 608, 901, 1316, 1414, 1441, 1502, 2123, 2224
Lsp1109I	GCAGC	8 cut(s)	729, 1057, 1406, 1450, 1478, 1841, 1918, 2138
MaeII	ACGT	7 cut(s)	57, 63, 721, 829, 906, 1655, 2148
MaeIII	GTNAC	1 cut(s)	618
MalI	GATC	9 cut(s)	205, 562, 630, 1117, 1458, 1564, 1603, 1661, 1780
MboI	GATC	9 cut(s)	203, 560, 628, 1115, 1456, 1562, 1601, 1659, 1778
MboII	GAAGA	6 cut(s)	456, 515, 887, 1625, 1685, 1743
MflI	RGATCY	5 cut(s)	560, 1115, 1456, 1601, 1778
MhlI	GDGCHC	5 cut(s)	327, 906, 1327, 1507, 1692
MlsI	TGGCCA	1 cut(s)	1860
MluNI	TGGCCA	1 cut(s)	1860
MlyI	GAGTC	1 cut(s)	841
MmeI	TCCRAC	6 cut(s)	224, 451, 743, 1149, 1623, 1852
Mox20I	TGGCCA	1 cut(s)	1860
Mph1103I	ATGCAT	2 cut(s)	715, 813
MroXI	GAANNNNTTC	3 cut(s)	118, 879, 1738
MscI	TGGCCA	1 cut(s)	1860
MseI	TTAA	9 cut(s)	159, 194, 366, 380, 653, 792, 1242, 1712, 2183
MslI	CAYNNNNRTG	3 cut(s)	933, 1866, 2272
Msp20I	TGGCCA	1 cut(s)	1860
MspA1I	CMGCKG	2 cut(s)	422, 1469
MspCI	CTTAAG	1 cut(s)	1241
MspR9I	CCNGG	2 cut(s)	960, 1510
Mva1269I	GAATGC	2 cut(s)	256, 2241
MvaI	CCWGG	2 cut(s)	960, 1510
MvnI	CGCG	1 cut(s)	2196
MwoI	GCNNNNNNNGC	6 cut(s)	347, 598, 1394, 2123, 2193, 2283
NdeI	CATATG	1 cut(s)	1269
NdeII	GATC	9 cut(s)	203, 560, 628, 1115, 1456, 1562, 1601, 1659, 1778
NheI	GCTAGC	1 cut(s)	833
NlaIII	CATG	6 cut(s)	527, 854, 932, 1211, 1448, 1682
NlaIV	GGNNCC	3 cut(s)	562, 1410, 1780
NmuCI	GTSAC	1 cut(s)	618
NruI	TCGCGA	1 cut(s)	2196
NsiI	ATGCAT	2 cut(s)	715, 813
NspI	RCATGY	1 cut(s)	1448
NspV	TTCGAA	3 cut(s)	179, 971, 2233
PaeR7I	CTCGAG	1 cut(s)	1521
PciSI	GCTCTTC	1 cut(s)	1668
PcsI	WCGNNNNNNNCGW	2 cut(s)	69, 977
PctI	GAATGC	2 cut(s)	256, 2241
PdmI	GAANNNNTTC	3 cut(s)	118, 879, 1738
PfeI	GAWTC	5 cut(s)	1093, 1472, 1616, 1739, 2165
PflMI	CCANNNNNTGG	3 cut(s)	557, 1405, 1499
PfoI	TCCNGGA	1 cut(s)	958
PkrI	GCNGC	8 cut(s)	744, 1072, 1396, 1465, 1468, 1831, 1908, 2128
PleI	GAGTC	1 cut(s)	841
PpsI	GAGTC	1 cut(s)	841
Ppu21I	YACGTR	1 cut(s)	907
PsiI	TTATAA	1 cut(s)	1290
Psp124BI	GAGCTC	1 cut(s)	327
Psp6I	CCWGG	2 cut(s)	958, 1508
PspGI	CCWGG	2 cut(s)	958, 1508
PspN4I	GGNNCC	3 cut(s)	562, 1410, 1780
PspPI	GGNCC	3 cut(s)	1193, 1595, 2132
PspXI	VCTCGAGB	1 cut(s)	1521
PstI	CTGCAG	1 cut(s)	1468
PsuI	RGATCY	5 cut(s)	560, 1115, 1456, 1601, 1778
PvuII	CAGCTG	2 cut(s)	422, 1469
RruI	TCGCGA	1 cut(s)	2196
RsaI	GTAC	3 cut(s)	295, 541, 1536
RsaNI	GTAC	3 cut(s)	294, 540, 1535
RseI	CAYNNNNRTG	3 cut(s)	933, 1866, 2272
SacI	GAGCTC	1 cut(s)	327
SalI	GTCGAC	1 cut(s)	59
SapI	GCTCTTC	1 cut(s)	1668
SaqAI	TTAA	9 cut(s)	159, 194, 366, 380, 653, 792, 1242, 1712, 2183
SatI	GCNGC	8 cut(s)	743, 1071, 1395, 1464, 1467, 1830, 1907, 2127
Sau3AI	GATC	9 cut(s)	203, 560, 628, 1115, 1456, 1562, 1601, 1659, 1778
Sau96I	GGNCC	3 cut(s)	1193, 1595, 2132
ScaI	AGTACT	1 cut(s)	1536
SchI	GAGTC	1 cut(s)	841
ScrFI	CCNGG	2 cut(s)	960, 1510
SduI	GDGCHC	5 cut(s)	327, 906, 1327, 1507, 1692
SfcI	CTRYAG	2 cut(s)	261, 1464
Sfr274I	CTCGAG	1 cut(s)	1521
SfuI	TTCGAA	3 cut(s)	179, 971, 2233
SgrDI	CGTCGACG	1 cut(s)	59
SinI	GGWCC	2 cut(s)	1595, 2132
SlaI	CTCGAG	1 cut(s)	1521
SmiMI	CAYNNNNRTG	3 cut(s)	933, 1866, 2272
SmlI	CTYRAG	3 cut(s)	1241, 1521, 1717
SmoI	CTYRAG	3 cut(s)	1241, 1521, 1717
SpeI	ACTAGT	1 cut(s)	2054
SsiI	CCGC	3 cut(s)	601, 702, 1229
SspI	AATATT	1 cut(s)	1696
SstI	GAGCTC	1 cut(s)	327
StyD4I	CCNGG	2 cut(s)	958, 1508
StyI	CCWWGG	1 cut(s)	1119
TaaI	ACNGT	4 cut(s)	824, 1181, 2018, 2025
TaiI	ACGT	7 cut(s)	60, 66, 724, 832, 909, 1658, 2151
TaqI	TCGA	9 cut(s)	60, 179, 358, 971, 1522, 1658, 1686, 1729, 2233
TatI	WGTACW	2 cut(s)	293, 1534
TfiI	GAWTC	5 cut(s)	1093, 1472, 1616, 1739, 2165
Tru1I	TTAA	9 cut(s)	159, 194, 366, 380, 653, 792, 1242, 1712, 2183
Tru9I	TTAA	9 cut(s)	159, 194, 366, 380, 653, 792, 1242, 1712, 2183
TscAI	CASTG	5 cut(s)	627, 756, 1327, 1548, 2030
TseFI	GTSAC	1 cut(s)	618
TseI	GCWGC	8 cut(s)	742, 1070, 1394, 1463, 1466, 1829, 1906, 2126
Tsp45I	GTSAC	1 cut(s)	618
TspDTI	ATGAA	7 cut(s)	932, 1256, 1686, 1715, 1818, 1856, 2016
TspGWI	ACGGA	4 cut(s)	232, 784, 2022, 2198
TspRI	CASTG	5 cut(s)	627, 756, 1327, 1548, 2030
Van91I	CCANNNNNTGG	3 cut(s)	557, 1405, 1499
Vha464I	CTTAAG	1 cut(s)	1241
VpaK11BI	GGWCC	2 cut(s)	1595, 2132
XagI	CCTNNNNNAGG	1 cut(s)	1056
XapI	RAATTY	8 cut(s)	175, 299, 643, 1382, 1417, 1722, 2079, 2217
XceI	RCATGY	1 cut(s)	1448
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1868
XhoI	CTCGAG	1 cut(s)	1521
XmiI	GTMKAC	4 cut(s)	60, 953, 1975, 2020
XmnI	GAANNNNTTC	3 cut(s)	118, 879, 1738
ZrmI	AGTACT	1 cut(s)	1536
Zsp2I	ATGCAT	2 cut(s)	715, 813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.