Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0007919

Physical Location & Seq

Genomic Coordinates Reverse (-)

7227241 .. 7238895

Gene Structure 11655 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00224060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGGAGGCCGTGCCTTTTCACGACGGCAACATCCACGACTTGATTGAGAGCCGATGCCTCGACGTTCGTCAAGATGTTAATCACAGCTTGCAGATGCACTCGTCATTGGTACGGCGGCTTTCTCAGGAGACGGAGTTGGAGGGGCATCAGGGTTGTGTTAATTCGATTGCTTGGAATTCTAGAGGTTCACTTTTAGTATCTGGATCAGACGACACACGGATAAATATTTGGAGCTGCTCTGGTCGGAAGCTTTTGCATTCTATTGAGACTGGGCACTGTGCAAACATATTTTGTACAAAATTTGTCCCTGAAACTTCTGATGAGCTTGTTGTCTCTGGAGCTGGAGATGCAGAAGTTCGGTTATTTAATTTGTCTCAGTTAAGTGGGAGAGGACCTGAAGATAATGCAGTTCCTCCATCAGCTCTGTATCAATGTCACACAAGAAGAGTAAAAAAGTTAGCTGTTGAAGTTGGGAATCCCAATGTGGTATGGAGTGCAAGTGAAGATGGAACTTTGAGACAGCATGACTTTCGAGAGGGTACTTCTTGCCCTCCTGCTGGATCTGCTCGTCAAGAATGTCGCAACATTTTACTTGACTTGCGGTCTGGAGCAAAGAAGTCACTAGCTGATCCCCCGAAACAAACATTTGCTCTAAAATCTTGTGATATCAGTTCCACTAGGCCTCATCTGCTATTAGTTGGTGGGAGTGATGCATTTGCACGTTTATATGATAGAAGGATGCTGCCTCCTCTGACATCCTGCCAGAAAAGGACGTCTCCACCTCCTTGTGTTAACTATTTCTGTCCGATGCATCTCTCTGATCATGGACGTACAAGCTTGCACTTAACTCACGTTACATTTAGCCCAGATGGAGAAGAGGTTCTACTTAGTTATAGTGGGGAGCATGTATATTTGATGAACGTAAATCATGCTGGTGGAAGTGCTGTGCAGTATACTTCAGGAGATGCTTCAAAGATAATGAGCTTCATACCTATGCTGAATGGGCTAGAACTGCAGCAACCAGCATCCAGTGTCTTCAGAAGTCGTCTTCCTAAAAGACGCAAGGCCACTGCTATGCATGAAAAGTGCAGTAAACTAACTCAAATTGCTGAGAAGTCCTTGGAGGAGGGGAATTTTTATCATGGTATAGAGGCATGTAATGAGGTGCTGGATGGATATGGTCGCGATATTGGGCCCGTACTAAGGCATGATTGTCTCTGTACTCGTGCCACATTGTTGCTGAAGCGTAATTGGAAAAACGATGCTCATATGGCTATAAGAGATTGCTATAATGCCCGGAAAATTGATAACTCCTCTTTCAGAGCTCATTACTATATGTCTGAAGCTTTATCACAGTTGGCCAAACATAAAGAAGCTGTAGAATTTGCTATGGCAGCGCAATCTTTAGCTCCGTCAAATCCTGAGGTAACAGCACAATTGGAGAGTGTAAAAAGAAAGCTTGCTGCAGCTGAGTCTGAGAGAAATGGTAAGCCAAATGATGGGGCACCCAGGTTTGAACCTCGGGGTGGAAGAGTGCTATCCCTTAGTGACATACTTTACCGATCTGAGGGTAATAGTGAAGCTTCACAAGATGGTCCAAGATCTGAAAGAGAGGATTCTGATTATGACGAGGAATTGGAGTTGGACTTTGAAACGTCCATCTCAGGTGATGAAGAGCAAGATATTGAGCCCAATATTCTACATGGAAGTGTAAATCTGAGAATTCATCGAAGAAGTGATTCTTCTAGAGAAGTTGAAGGGGCAAATGGCTCTTGTGGATCACCTTCATCATCATCTCAAAATGAAAGACTCCCATATCAGCCTGCGGCAGTTATTGATATGAAACAGAGATATGTCGGTCATTGTAATGTTGGAACTGACATAAAACAAGCCAGCTTTCTTGGCCAGAGAGGTGAATATGTTGCTAGTGGAAGTGATGATGGCAGATGGTTTATCTGGGAGAAACGAACTGGCAGACTAATAAAAATGCTTCAAGGAGATGAAGCTGTTGTGAACTGTGTACAGTGCCATCCCTCCGATTGTGTTGTGGCAACTAGTGGGATTGACAGTACAATAAAGTTATGGACCCCAAGTGCTTCAGTCCCATCTATTGTAGCTGGTGGAGCCGCTGGACTAGAAACTTCTAACATTTCTAGTGTTTTGTCAGCTAATCAGCGCAGATTAAGCCATAATCGTGAAACCATCCTGTACAGCCGTTCTGAAATTTTGGAGCATTTTCGAATGCATGAATTTACTGAAGGAAGCTTGCGCCCGTTTGAGTGTGCTCAGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0019538 GO:0031974 GO:0031981 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

764

Amino Acids

84.34

Weight (kDa)

6.15

Isoelectric Point (pI)

64.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	30 - 125	4.9e-10	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	32 - 126	4.8e-07	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	38 - 181	1.2e-17	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	41 - 126	4.8e-13	WDR3 first beta-propeller domain
WD40	PF00400	43 - 78	1.1e-08	WD domain, G-beta repeat
EIF3I	PF24805	46 - 125	4.6e-06	EIF3I
Beta-prop_THOC3	PF25174	49 - 126	6.7e-11	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	53 - 126	9.2e-06	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	614 - 696	1e-12	WDR5 beta-propeller domain
WD40_Prp19	PF24814	631 - 700	2.5e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	636 - 696	1.9e-06	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	637 - 696	8.4e-07	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0007919)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10940 AT5G10940
fragaria_vesca	FvH4_5g35830 FvH4_5g35830 FvH4_5g35830
malus_domestica	MD08G1192800.v1.1 MD15G1381500.v1.1
prunus_persica	Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1
pyrus_communis	pycom08g16630 pycom15g34170
rosa_chinensis	RchiOBHm_Chr7g0237521
rosa_laevigata	RLG00000000996
rosa_multiflora	Rmu_ssc0000152.1_g000001
rosa_roxburghii	Rroxscaffold_3G00224060
rosa_rugosa	Rorug07G0303800 Rorug07G0303900
rosa_samantha	Rh7AG461500 Rh7BG431700 Rh7CG478700 Rh7DG447900
rosa_wichuraiana	Rw7G038190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	776
AccB1I	GGYRCC	1 cut(s)	1504
AccB7I	CCANNNNNTGG	1 cut(s)	1499
AccI	GTMKAC	1 cut(s)	953
AccII	CGCG	1 cut(s)	1185
AciI	CCGC	4 cut(s)	115, 601, 1826, 2127
AclWI	GGATC	4 cut(s)	211, 568, 623, 1786
AcoI	YGGCCR	2 cut(s)	1359, 1903
AcsI	RAATTY	7 cut(s)	175, 299, 1132, 1382, 1722, 2223, 2249
AcuI	CTGAAG	7 cut(s)	417, 942, 1021, 1262, 1362, 2082, 2277
AcyI	GRCGYC	1 cut(s)	773
AfaI	GTAC	9 cut(s)	111, 295, 541, 832, 1200, 1222, 2022, 2071, 2210
AfiI	CCNNNNNNNGG	5 cut(s)	557, 1203, 1499, 1526, 1567
AgsI	TTSAA	6 cut(s)	467, 972, 1517, 1652, 1757, 1994
AhdI	GACNNNNNGTC	1 cut(s)	601
AhlI	ACTAGT	1 cut(s)	2054
AjnI	CCWGG	1 cut(s)	1508
AloI	GAACNNNNNNTCC	2 cut(s)	864, 896
Alw21I	GWGCWC	2 cut(s)	1327, 2287
Alw26I	GTCTC	7 cut(s)	122, 260, 337, 378, 511, 780, 1220
AlwI	GGATC	4 cut(s)	211, 568, 623, 1786
Ama87I	CYCGRG	1 cut(s)	1521
AoxI	GGCC	6 cut(s)	6, 680, 1065, 1193, 1359, 1903
ApaI	GGGCCC	1 cut(s)	1197
ApeKI	GCWGC	6 cut(s)	234, 742, 1015, 1394, 1463, 1466
ApoI	RAATTY	7 cut(s)	175, 299, 1132, 1382, 1722, 2223, 2249
Asp700I	GAANNNNTTC	2 cut(s)	879, 1738
AspLEI	GCGC	3 cut(s)	1399, 2178, 2271
AspS9I	GGNCC	5 cut(s)	392, 1193, 1194, 1595, 2085
AsuC2I	CCSGG	1 cut(s)	1297
AsuHPI	GGTGA	3 cut(s)	1679, 1773, 1925
AsuII	TTCGAA	1 cut(s)	2239
AvaI	CYCGRG	1 cut(s)	1521
AvaII	GGWCC	3 cut(s)	392, 1595, 2085
AxyI	CCTNAGG	1 cut(s)	1422
BaeGI	GKGCMC	3 cut(s)	276, 1197, 1507
BalI	TGGCCA	2 cut(s)	1361, 1905
BanI	GGYRCC	1 cut(s)	1504
BanII	GRGCYC	3 cut(s)	1197, 1327, 1692
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
BauI	CACGAG	1 cut(s)	1224
BbsI	GAAGAC	2 cut(s)	1027, 1040
Bbv12I	GWGCWC	2 cut(s)	1327, 2287
BbvI	GCAGC	6 cut(s)	221, 729, 1027, 1406, 1450, 1478
BceAI	ACGGC	3 cut(s)	40, 128, 2199
BcgI	CGANNNNNNTGC	2 cut(s)	512, 546
BciT130I	CCWGG	1 cut(s)	1510
BclI	TGATCA	1 cut(s)	820
BcnI	CCSGG	1 cut(s)	1297
BcoDI	GTCTC	7 cut(s)	122, 260, 337, 378, 511, 780, 1220
BcuI	ACTAGT	1 cut(s)	2054
BfaI	CTAG	9 cut(s)	180, 623, 678, 1007, 1746, 1926, 2055, 2135, 2154
BfmI	CTRYAG	3 cut(s)	1013, 1377, 1464
BglII	AGATCT	1 cut(s)	1601
BisI	GCNGC	9 cut(s)	116, 235, 743, 1016, 1395, 1464, 1467, 1827, 2127
BlsI	GCNGC	9 cut(s)	117, 236, 744, 1017, 1396, 1465, 1468, 1828, 2128
Bme1390I	CCNGG	2 cut(s)	1297, 1510
Bme18I	GGWCC	3 cut(s)	392, 1595, 2085
BmeRI	GACNNNNNGTC	1 cut(s)	601
BmeT110I	CYCGRG	1 cut(s)	1521
BmgT120I	GGNCC	5 cut(s)	392, 1193, 1194, 1595, 2085
BmiI	GGNNCC	4 cut(s)	1195, 1506, 2087, 2125
BmrFI	CCNGG	2 cut(s)	1297, 1510
BmrI	ACTGGG	1 cut(s)	279
BmuI	ACTGGG	1 cut(s)	279
BoxI	GACNNNNGTC	1 cut(s)	66
BpiI	GAAGAC	2 cut(s)	1027, 1040
BpmI	CTGGAG	3 cut(s)	357, 363, 627
Bpu14I	TTCGAA	1 cut(s)	2239
BpuMI	CCSGG	1 cut(s)	1297
BsaBI	GATNNNNATC	1 cut(s)	78
BsaHI	GRCGYC	1 cut(s)	773
BsaJI	CCNNGG	3 cut(s)	1119, 1508, 1520
BsaXI	ACNNNNNCTCC	6 cut(s)	119, 149, 484, 514, 864, 894
Bsc4I	CCNNNNNNNGG	5 cut(s)	557, 1203, 1499, 1526, 1567
Bse1I	ACTGG	3 cut(s)	274, 1029, 1975
Bse21I	CCTNAGG	1 cut(s)	1422
Bse8I	GATNNNNATC	1 cut(s)	78
BseBI	CCWGG	1 cut(s)	1510
BseDI	CCNNGG	3 cut(s)	1119, 1508, 1520
BseGI	GGATG	7 cut(s)	30, 744, 755, 1025, 1177, 2029, 2202
BseJI	GATNNNNATC	1 cut(s)	78
BseLI	CCNNNNNNNGG	5 cut(s)	557, 1203, 1499, 1526, 1567
BseMII	CTCAG	9 cut(s)	137, 389, 1101, 1413, 1461, 1467, 1557, 1677, 1709
BseNI	ACTGG	3 cut(s)	274, 1029, 1975
BseRI	GAGGAG	3 cut(s)	738, 1139, 1303
BseSI	GKGCMC	3 cut(s)	276, 1197, 1507
BseXI	GCAGC	6 cut(s)	221, 729, 1027, 1406, 1450, 1478
BsgI	GTGCAG	2 cut(s)	968, 1108
Bsh1236I	CGCG	1 cut(s)	1185
BshFI	GGCC	6 cut(s)	8, 682, 1067, 1195, 1361, 1905
BshNI	GGYRCC	1 cut(s)	1504
BsiHKAI	GWGCWC	2 cut(s)	1327, 2287
BsiHKCI	CYCGRG	1 cut(s)	1521
BsiSI	CCGG	1 cut(s)	1297
BslFI	GGGAC	2 cut(s)	290, 2087
BslI	CCNNNNNNNGG	5 cut(s)	557, 1203, 1499, 1526, 1567
BsmAI	GTCTC	7 cut(s)	122, 260, 337, 378, 511, 780, 1220
BsmBI	CGTCTC	2 cut(s)	122, 780
BsmFI	GGGAC	2 cut(s)	290, 2087
BsmI	GAATGC	2 cut(s)	256, 2247
BsnI	GGCC	6 cut(s)	8, 682, 1067, 1195, 1361, 1905
BsoBI	CYCGRG	1 cut(s)	1521
Bsp119I	TTCGAA	1 cut(s)	2239
Bsp120I	GGGCCC	1 cut(s)	1193
Bsp1286I	GDGCHC	6 cut(s)	276, 1197, 1327, 1507, 1692, 2287
Bsp1407I	TGTACA	3 cut(s)	293, 2020, 2208
Bsp143I	GATC	7 cut(s)	203, 560, 628, 820, 1562, 1601, 1778
Bsp68I	TCGCGA	1 cut(s)	1185
BspACI	CCGC	4 cut(s)	115, 601, 1826, 2127
BspANI	GGCC	6 cut(s)	8, 682, 1067, 1195, 1361, 1905
BspCNI	CTCAG	9 cut(s)	136, 388, 1102, 1414, 1462, 1468, 1558, 1676, 1710
BspFNI	CGCG	1 cut(s)	1185
BspLI	GGNNCC	4 cut(s)	1195, 1506, 2087, 2125
BspMAI	CTGCAG	2 cut(s)	1017, 1468
BspPI	GGATC	4 cut(s)	211, 568, 623, 1786
BspQI	GCTCTTC	1 cut(s)	1668
BspT104I	TTCGAA	1 cut(s)	2239
BspT107I	GGYRCC	1 cut(s)	1504
BsrGI	TGTACA	3 cut(s)	293, 2020, 2208
BsrI	ACTGG	3 cut(s)	274, 1029, 1975
BssECI	CCNNGG	3 cut(s)	1119, 1508, 1520
BssMI	GATC	7 cut(s)	203, 560, 628, 820, 1562, 1601, 1778
BssNAI	GTATAC	1 cut(s)	954
BssNI	GRCGYC	1 cut(s)	773
BssSI	CACGAG	1 cut(s)	1224
BssT1I	CCWWGG	1 cut(s)	1119
Bst1107I	GTATAC	1 cut(s)	954
Bst2BI	CACGAG	1 cut(s)	1224
Bst2UI	CCWGG	1 cut(s)	1510
Bst4CI	ACNGT	5 cut(s)	278, 1356, 2018, 2025, 2069
Bst6I	CTCTTC	4 cut(s)	439, 870, 1525, 1668
BstACI	GRCGYC	1 cut(s)	773
BstAUI	TGTACA	3 cut(s)	293, 2020, 2208
BstBI	TTCGAA	1 cut(s)	2239
BstC8I	GCNNGC	6 cut(s)	89, 839, 1461, 1824, 1894, 2267
BstF5I	GGATG	7 cut(s)	30, 744, 755, 1025, 1177, 2029, 2202
BstFNI	CGCG	1 cut(s)	1185
BstHHI	GCGC	3 cut(s)	1399, 2178, 2271
BstKTI	GATC	7 cut(s)	206, 563, 631, 823, 1565, 1604, 1781
BstMAI	GTCTC	7 cut(s)	122, 260, 337, 378, 511, 780, 1220
BstMBI	GATC	7 cut(s)	203, 560, 628, 820, 1562, 1601, 1778
BstNI	CCWGG	1 cut(s)	1510
BstNSI	RCATGY	2 cut(s)	908, 1158
BstPAI	GACNNNNGTC	1 cut(s)	66
BstSCI	CCNGG	2 cut(s)	1295, 1508
BstSFI	CTRYAG	3 cut(s)	1013, 1377, 1464
BstSLI	GKGCMC	3 cut(s)	276, 1197, 1507
BstUI	CGCG	1 cut(s)	1185
BstV1I	GCAGC	6 cut(s)	221, 729, 1027, 1406, 1450, 1478
BstV2I	GAAGAC	2 cut(s)	1027, 1040
BstX2I	RGATCY	2 cut(s)	560, 1601
BstYI	RGATCY	2 cut(s)	560, 1601
BstZ17I	GTATAC	1 cut(s)	954
Bsu36I	CCTNAGG	1 cut(s)	1422
BsuRI	GGCC	6 cut(s)	8, 682, 1067, 1195, 1361, 1905
BtsCI	GGATG	7 cut(s)	30, 744, 755, 1025, 1177, 2029, 2202
BtsI	GCAGTG	1 cut(s)	1068
BtsIMutI	CAGTG	4 cut(s)	274, 1036, 1068, 2030
BtuMI	TCGCGA	1 cut(s)	1185
Cac8I	GCNNGC	6 cut(s)	89, 839, 1461, 1824, 1894, 2267
CfoI	GCGC	3 cut(s)	1399, 2178, 2271
Cfr13I	GGNCC	5 cut(s)	392, 1193, 1194, 1595, 2085
CseI	GACGC	1 cut(s)	1068
Csp6I	GTAC	9 cut(s)	110, 294, 540, 831, 1199, 1221, 2021, 2070, 2209
CviQI	GTAC	9 cut(s)	110, 294, 540, 831, 1199, 1221, 2021, 2070, 2209
DpnI	GATC	7 cut(s)	205, 562, 630, 822, 1564, 1603, 1780
DpnII	GATC	7 cut(s)	203, 560, 628, 820, 1562, 1601, 1778
DriI	GACNNNNNGTC	1 cut(s)	601
EaeI	YGGCCR	2 cut(s)	1359, 1903
Eam1104I	CTCTTC	4 cut(s)	439, 870, 1525, 1668
Eam1105I	GACNNNNNGTC	1 cut(s)	601
EarI	CTCTTC	4 cut(s)	439, 870, 1525, 1668
Ecl136II	GAGCTC	1 cut(s)	1325
Eco130I	CCWWGG	1 cut(s)	1119
Eco147I	AGGCCT	1 cut(s)	682
Eco24I	GRGCYC	3 cut(s)	1197, 1327, 1692
Eco32I	GATATC	1 cut(s)	667
Eco47I	GGWCC	3 cut(s)	392, 1595, 2085
Eco53kI	GAGCTC	1 cut(s)	1325
Eco57I	CTGAAG	7 cut(s)	417, 942, 1021, 1262, 1362, 2082, 2277
Eco81I	CCTNAGG	1 cut(s)	1422
Eco88I	CYCGRG	1 cut(s)	1521
EcoICRI	GAGCTC	1 cut(s)	1325
EcoO109I	RGGNCCY	1 cut(s)	392
EcoRI	GAATTC	2 cut(s)	175, 1722
EcoRII	CCWGG	1 cut(s)	1508
EcoRV	GATATC	1 cut(s)	667
EcoT14I	CCWWGG	1 cut(s)	1119
EcoT22I	ATGCAT	4 cut(s)	715, 813, 1080, 2247
EcoT38I	GRGCYC	3 cut(s)	1197, 1327, 1692
ErhI	CCWWGG	1 cut(s)	1119
Esp3I	CGTCTC	2 cut(s)	122, 780
FalI	AAGNNNNNCTT	2 cut(s)	1726, 1758
FaqI	GGGAC	2 cut(s)	290, 2087
FauNDI	CATATG	1 cut(s)	1269
FbaI	TGATCA	1 cut(s)	820
FblI	GTMKAC	1 cut(s)	953
Fnu4HI	GCNGC	9 cut(s)	116, 235, 743, 1016, 1395, 1464, 1467, 1827, 2127
FokI	GGATG	7 cut(s)	17, 742, 751, 1012, 1184, 2016, 2189
FriOI	GRGCYC	3 cut(s)	1197, 1327, 1692
Fsp4HI	GCNGC	9 cut(s)	116, 235, 743, 1016, 1395, 1464, 1467, 1827, 2127
FspBI	CTAG	9 cut(s)	180, 623, 678, 1007, 1746, 1926, 2055, 2135, 2154
GlaI	GCGC	3 cut(s)	1398, 2177, 2270
GluI	GCNGC	9 cut(s)	116, 235, 743, 1016, 1395, 1464, 1467, 1827, 2127
GsuI	CTGGAG	3 cut(s)	357, 363, 627
HaeIII	GGCC	6 cut(s)	8, 682, 1067, 1195, 1361, 1905
HapII	CCGG	1 cut(s)	1297
HgaI	GACGC	1 cut(s)	1068
HhaI	GCGC	3 cut(s)	1399, 2178, 2271
Hin1I	GRCGYC	1 cut(s)	773
Hin6I	GCGC	3 cut(s)	1397, 2176, 2269
HinP1I	GCGC	3 cut(s)	1397, 2176, 2269
HincII	GTYRAC	1 cut(s)	793
HindII	GTYRAC	1 cut(s)	793
HindIII	AAGCTT	6 cut(s)	248, 835, 1344, 1457, 1581, 2263
HinfI	GANTC	5 cut(s)	475, 1472, 1616, 1739, 1809
HpaI	GTTAAC	1 cut(s)	793
HpaII	CCGG	1 cut(s)	1297
HphI	GGTGA	3 cut(s)	1679, 1773, 1925
Hpy166II	GTNNAC	6 cut(s)	188, 793, 954, 1094, 2014, 2021
Hpy8I	GTNNAC	6 cut(s)	188, 793, 954, 1094, 2014, 2021
Hpy99I	CGWCG	2 cut(s)	26, 65
HpyAV	CCTTC	4 cut(s)	729, 1751, 1794, 2252
HpyCH4III	ACNGT	5 cut(s)	278, 1356, 2018, 2025, 2069
HpyCH4IV	ACGT	7 cut(s)	63, 721, 773, 829, 852, 921, 1655
HpySE526I	ACGT	7 cut(s)	63, 721, 773, 829, 852, 921, 1655
Hsp92I	GRCGYC	1 cut(s)	773
HspAI	GCGC	3 cut(s)	1397, 2176, 2269
Ksp22I	TGATCA	1 cut(s)	820
KspAI	GTTAAC	1 cut(s)	793
Kzo9I	GATC	7 cut(s)	203, 560, 628, 820, 1562, 1601, 1778
LguI	GCTCTTC	1 cut(s)	1668
LmnI	GCTCC	7 cut(s)	231, 338, 608, 901, 1414, 2123, 2230
Lsp1109I	GCAGC	6 cut(s)	221, 729, 1027, 1406, 1450, 1478
MaeI	CTAG	9 cut(s)	180, 623, 678, 1007, 1746, 1926, 2055, 2135, 2154
MaeII	ACGT	7 cut(s)	63, 721, 773, 829, 852, 921, 1655
MaeIII	GTNAC	5 cut(s)	434, 618, 853, 1426, 1547
MalI	GATC	7 cut(s)	205, 562, 630, 822, 1564, 1603, 1780
MboI	GATC	7 cut(s)	203, 560, 628, 820, 1562, 1601, 1778
MfeI	CAATTG	1 cut(s)	1436
MflI	RGATCY	2 cut(s)	560, 1601
MhlI	GDGCHC	6 cut(s)	276, 1197, 1327, 1507, 1692, 2287
MlsI	TGGCCA	2 cut(s)	1361, 1905
MluNI	TGGCCA	2 cut(s)	1361, 1905
MlyI	GAGTC	2 cut(s)	1481, 1803
MmeI	TCCRAC	4 cut(s)	117, 224, 1623, 1852
Mox20I	TGGCCA	2 cut(s)	1361, 1905
Mph1103I	ATGCAT	4 cut(s)	715, 813, 1080, 2247
MroXI	GAANNNNTTC	2 cut(s)	879, 1738
MscI	TGGCCA	2 cut(s)	1361, 1905
MseI	TTAA	7 cut(s)	78, 159, 366, 380, 792, 845, 2183
MslI	CAYNNNNRTG	6 cut(s)	933, 992, 1073, 1707, 1866, 2193
Msp20I	TGGCCA	2 cut(s)	1361, 1905
MspA1I	CMGCKG	2 cut(s)	1469, 2129
MspI	CCGG	1 cut(s)	1297
MspR9I	CCNGG	2 cut(s)	1297, 1510
MunI	CAATTG	1 cut(s)	1436
Mva1269I	GAATGC	2 cut(s)	256, 2247
MvaI	CCWGG	1 cut(s)	1510
MvnI	CGCG	1 cut(s)	1185
NciI	CCSGG	1 cut(s)	1297
NdeI	CATATG	1 cut(s)	1269
NdeII	GATC	7 cut(s)	203, 560, 628, 820, 1562, 1601, 1778
NlaIV	GGNNCC	4 cut(s)	1195, 1506, 2087, 2125
NmuCI	GTSAC	3 cut(s)	434, 618, 1547
NruI	TCGCGA	1 cut(s)	1185
NsiI	ATGCAT	4 cut(s)	715, 813, 1080, 2247
NspI	RCATGY	2 cut(s)	908, 1158
NspV	TTCGAA	1 cut(s)	2239
PceI	AGGCCT	1 cut(s)	682
PciSI	GCTCTTC	1 cut(s)	1668
PctI	GAATGC	2 cut(s)	256, 2247
PdmI	GAANNNNTTC	2 cut(s)	879, 1738
PfeI	GAWTC	3 cut(s)	475, 1616, 1739
PflMI	CCANNNNNTGG	1 cut(s)	1499
PkrI	GCNGC	9 cut(s)	117, 236, 744, 1017, 1396, 1465, 1468, 1828, 2128
PleI	GAGTC	2 cut(s)	1480, 1803
PpsI	GAGTC	2 cut(s)	1480, 1803
PpuMI	RGGWCCY	1 cut(s)	392
PshAI	GACNNNNGTC	1 cut(s)	66
Psp124BI	GAGCTC	1 cut(s)	1327
Psp5II	RGGWCCY	1 cut(s)	392
Psp6I	CCWGG	1 cut(s)	1508
PspGI	CCWGG	1 cut(s)	1508
PspN4I	GGNNCC	4 cut(s)	1195, 1506, 2087, 2125
PspOMI	GGGCCC	1 cut(s)	1193
PspPI	GGNCC	5 cut(s)	392, 1193, 1194, 1595, 2085
PspPPI	RGGWCCY	1 cut(s)	392
PstI	CTGCAG	2 cut(s)	1017, 1468
PsuI	RGATCY	2 cut(s)	560, 1601
PvuII	CAGCTG	1 cut(s)	1469
RruI	TCGCGA	1 cut(s)	1185
RsaI	GTAC	9 cut(s)	111, 295, 541, 832, 1200, 1222, 2022, 2071, 2210
RsaNI	GTAC	9 cut(s)	110, 294, 540, 831, 1199, 1221, 2021, 2070, 2209
RseI	CAYNNNNRTG	6 cut(s)	933, 992, 1073, 1707, 1866, 2193
SacI	GAGCTC	1 cut(s)	1327
SapI	GCTCTTC	1 cut(s)	1668
SaqAI	TTAA	7 cut(s)	78, 159, 366, 380, 792, 845, 2183
SatI	GCNGC	9 cut(s)	116, 235, 743, 1016, 1395, 1464, 1467, 1827, 2127
Sau3AI	GATC	7 cut(s)	203, 560, 628, 820, 1562, 1601, 1778
Sau96I	GGNCC	5 cut(s)	392, 1193, 1194, 1595, 2085
SchI	GAGTC	2 cut(s)	1481, 1803
ScrFI	CCNGG	2 cut(s)	1297, 1510
SduI	GDGCHC	6 cut(s)	276, 1197, 1327, 1507, 1692, 2287
SfcI	CTRYAG	3 cut(s)	1013, 1377, 1464
SfuI	TTCGAA	1 cut(s)	2239
SinI	GGWCC	3 cut(s)	392, 1595, 2085
SmiMI	CAYNNNNRTG	6 cut(s)	933, 992, 1073, 1707, 1866, 2193
SpeI	ACTAGT	1 cut(s)	2054
SseBI	AGGCCT	1 cut(s)	682
SsiI	CCGC	4 cut(s)	115, 601, 1826, 2127
SspI	AATATT	2 cut(s)	226, 1696
SspMI	CTAG	9 cut(s)	180, 623, 678, 1007, 1746, 1926, 2055, 2135, 2154
SstI	GAGCTC	1 cut(s)	1327
StuI	AGGCCT	1 cut(s)	682
StyD4I	CCNGG	2 cut(s)	1295, 1508
StyI	CCWWGG	1 cut(s)	1119
TaaI	ACNGT	5 cut(s)	278, 1356, 2018, 2025, 2069
TaiI	ACGT	7 cut(s)	66, 724, 776, 832, 855, 924, 1658
TaqI	TCGA	5 cut(s)	60, 164, 532, 1729, 2239
TaqII	GACCGA	1 cut(s)	1847
TatI	WGTACW	5 cut(s)	293, 1220, 2020, 2069, 2208
TauI	GCSGC	3 cut(s)	118, 1829, 2129
TfiI	GAWTC	3 cut(s)	475, 1616, 1739
Tru1I	TTAA	7 cut(s)	78, 159, 366, 380, 792, 845, 2183
Tru9I	TTAA	7 cut(s)	78, 159, 366, 380, 792, 845, 2183
TscAI	CASTG	4 cut(s)	281, 1036, 1075, 2030
TseFI	GTSAC	3 cut(s)	434, 618, 1547
TseI	GCWGC	6 cut(s)	234, 742, 1015, 1394, 1463, 1466
Tsp45I	GTSAC	3 cut(s)	434, 618, 1547
TspGWI	ACGGA	3 cut(s)	146, 232, 1401
TspRI	CASTG	4 cut(s)	281, 1036, 1075, 2030
Van91I	CCANNNNNTGG	1 cut(s)	1499
VpaK11BI	GGWCC	3 cut(s)	392, 1595, 2085
XapI	RAATTY	7 cut(s)	175, 299, 1132, 1382, 1722, 2223, 2249
XbaI	TCTAGA	2 cut(s)	179, 1745
XceI	RCATGY	2 cut(s)	908, 1158
XmiI	GTMKAC	1 cut(s)	953
XmnI	GAANNNNTTC	2 cut(s)	879, 1738
XspI	CTAG	9 cut(s)	180, 623, 678, 1007, 1746, 1926, 2055, 2135, 2154
ZraI	GACGTC	1 cut(s)	774
Zsp2I	ATGCAT	4 cut(s)	715, 813, 1080, 2247

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.