MD15G1381500.v1.1

WD and tetratricopeptide repeats protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007919
Physical Location & Seq
Reverse (-)
47020486 .. 47032874
12389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1381500.v1.1.491

Sequence Viewer

Length: 2214 bp
ATGGAAGGCGTCCCTTTTCACGACGGCAACATCCACGACTTGATTGCGAGACGATACGTCGATGTTCGGCACGATGCCAATCACAGGTTGCAGATGCACTCGTCGTTGGTGCGAAGGCTTTCTCAGGAAAGAGAATTGGAGGGGCATCAAGGTTGTGTTAATTCCATTGCTTGGAATTCGAATGGCTCGCTTTTAATGTCTGGATCAGATGACACACGGATAAATATCTGGAGCTACTCTAGTCGGAAGCTTTTGCATTCTATAGAGACTGGGCACAGTGCGAACATATTTTGTACAAAATTTGTCCCTGAAACTTCTGATGAGCTTGTGGTCTCTGGAGCTGGAGATGCAGAAGTTCGATTATTTAATTTGTCTCGTTTAAGTGGGAGAGGAACTGATGATAATACTATTTCTCCATCAGCTGTGTATCAATGCCATAGTAGAAGAGTAAAAAAGTTAGCTGTTGAAGTTGGAAATCCCAATGTGGTATGGAGTGCAAGTGAAGATGGAACTTTGAGACAGCATGACTTCCGAGAGGGCACTTCTTGCCCACCTGCTGGATCCACTAATCAAGAATGTCGCAATGTTTTGCTTGACTTGCGGTGTGGAGCAAAGAAGTCACTTGCTGATCCCCCCAAGCAAAATTTTTCTTTAAAATCTTGTGATATCAGTTCCACTAGACCTCATTTACTCCTAGTTGGTGGGAGTGATGCATTTGCCCGTTTATATGATAGAAGGATGCTGCCACCACTGACATCCTGTCGGAAACGGACAGCTCCACCTCCTTGTGTTAACTATTTTTGTCCAATGCATCTCTCCGAACGTGGACGTGCTAGCTTGCATTTGACTCATGTTGCATTTAGTCCAGATGGAGAAGAGGTTCTACTTAGTTATAGTGGGGAGCACGTATATTTGATGAATGTAAATCATGCTGGTGAGAGTGCTATGCAGTATACTTCTGGAGATGTTTTGAAACTAACCAGCTTCAGTCCTATACTCAATGGTGTCGAACTGCATCAACCAGCATCCAATGTGTTCAGAAATGGTCTTCCCAAAAGAGGCAACGTTGCTGCTAGGTTTGAGAAGTGCAGGAATCTAATTGAAATTGCTGAGCGAGCCTTGGAGGAGGGGACAGATTATTTTTATGGCATTGAAGCGTGCAATGAGGTGTTGGATGGAAACGGTTGTGATATTGGGCCTACACTAAAGCATGATTGCCTTTGTATTCGTGCGGCATTGTTGCTTAAGCGCAAGTGGAAAAATGATGTTCATATGGCTATAAGAGATTGTTATAATGCTCGGAGGCTCATTAGCTCCTCTTACAGAGCTCATTACTATATGTCCAAAGCTTTGTCGCAGTTGGCTAAACATAAAGAAGCTCTGGAATTTGCTATCGCAGCCCAATCTTTAGCTCCATCAAATTCTCAAGTAGCAGAACGATTGGAGCGTGTACAAAGGGATCTTGCTGCAGCTGAATCTGAAAGAAATAGTAAGCCAAATGATGGAGCACCCAGGTCTGAACCTCGAGGTGAAAGAGTTCTATCTCTGAGTGACATACTCTACCGATCAGAGGGTAATAGTGATGTTTCACAAGATGGTCCAAGATCTGAAAGAGAAGATTCTGATTATGATGAGGAACTAGAGCTGGACTTTGAAACGTCGATCTCAGGTGATGAAGAGCATGACATCGAGCCCAACATTGTACAAGGAAGCTTAAACTTGAGAATTCATCGAAGAAGTGATTCTGCTAGAGAAGTTGGTGGTGCAAACGGCCCATCTGGATCCCCTTCTTCGTCATCTCAAAATGAAAGAATACCATATCAGCCTGAGGCAGTTATTGATATGAAACAGAGATATGTTGGCCATTGTAATGTTGGAACGGACATAAAACAGGCCAGCTTTCTTGGCCAGCGAGGTGAATATGTTGCTAGCGGAAGTGATGATGGCAGATGGTTTGTCTGGGAGAAGCGAACTGGTAGACTGATAAAAATGCTTCAAGGAGATGAAGCTGTTGTGAACTGTGTACAGTGCCATCCGGTTGATTGTGTTGTGGCAACTAGTGGAATTGACAACACAATAAAAATTTGGACCCCCAGTGCCTCAGTCCCATCTATTGTGGCTGGTGGAGCCGCGGGACCAGAAAATGCTAACATTTCGGTAGTCATGGAATCCAATCAGCGCAGATTAAGCCATAATCGTGAGACTATCCTGTAA

Protein Analysis

738

Amino Acids

81.5

Weight (kDa)

6.19

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 27 - 125 1.2e-10 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 32 - 125 1.6e-07 TEP-1 second beta-propeller
ANAPC4_WD40 PF12894 37 - 99 7.4e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR5 PF25175 38 - 181 1.5e-19 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 41 - 125 2.2e-13 WDR3 first beta-propeller domain
WDR55 PF24796 42 - 180 2.9e-07 WDR55
WD40 PF00400 43 - 78 3.8e-09 WD domain, G-beta repeat
EIF3I PF24805 46 - 125 7.4e-07 EIF3I
Beta-prop_THOC3 PF25174 49 - 125 4.5e-12 THOC3 beta-propeller domain
WD40_Prp19 PF24814 53 - 182 1.1e-11 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 53 - 126 2.7e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 615 - 697 8.2e-13 WDR5 beta-propeller domain
WD40_Prp19 PF24814 631 - 701 3.8e-07 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 637 - 697 4.4e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 638 - 699 1.4e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1293
AarI CACCTGC 1 cut(s) 562
AbsI CCTCGAGG 1 cut(s) 1524
Acc36I ACCTGC 1 cut(s) 562
AccB7I CCANNNNNTGG 3 cut(s) 171, 557, 1502
AccI GTMKAC 2 cut(s) 953, 1978
AccII CGCG 1 cut(s) 2132
AciI CCGC 5 cut(s) 601, 1232, 1932, 2130, 2132
AclI AACGTT 1 cut(s) 1065
AclWI GGATC 7 cut(s) 211, 555, 568, 623, 1467, 1776, 1789
AcoI YGGCCR 2 cut(s) 1861, 1906
AcsI RAATTY 7 cut(s) 175, 299, 643, 1385, 1420, 1725, 2082
AcuI CTGAAG 1 cut(s) 970
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 4 cut(s) 295, 1452, 1704, 2025
AfiI CCNNNNNNNGG 6 cut(s) 84, 171, 557, 1058, 1502, 1570
AflII CTTAAG 1 cut(s) 1244
AgsI TTSAA 6 cut(s) 467, 973, 1103, 1154, 1655, 1997
AhdI GACNNNNNGTC 2 cut(s) 56, 759
AhlI ACTAGT 1 cut(s) 2057
AjiI CACGTC 1 cut(s) 830
AjnI CCWGG 1 cut(s) 1511
AloI GAACNNNNNNTCC 2 cut(s) 864, 896
Alw21I GWGCWC 3 cut(s) 906, 1330, 1510
Alw26I GTCTC 6 cut(s) 43, 260, 337, 378, 511, 2195
AlwI GGATC 7 cut(s) 211, 555, 568, 623, 1467, 1776, 1789
Ama87I CYCGRG 1 cut(s) 1524
AoxI GGCC 5 cut(s) 1196, 1771, 1861, 1893, 1906
ApeKI GCWGC 5 cut(s) 742, 1070, 1397, 1466, 1469
ApoI RAATTY 7 cut(s) 175, 299, 643, 1385, 1420, 1725, 2082
Asp700I GAANNNNTTC 4 cut(s) 118, 879, 1536, 1741
AspLEI GCGC 2 cut(s) 1251, 2181
AspS9I GGNCC 5 cut(s) 1196, 1598, 1772, 2088, 2135
AsuHPI GGTGA 4 cut(s) 947, 1541, 1682, 1928
AsuII TTCGAA 1 cut(s) 179
AsuNHI GCTAGC 2 cut(s) 833, 1928
AvaI CYCGRG 1 cut(s) 1524
AvaII GGWCC 3 cut(s) 1598, 2088, 2135
AxyI CCTNAGG 1 cut(s) 1827
BaeGI GKGCMC 2 cut(s) 276, 542
BalI TGGCCA 2 cut(s) 1863, 1908
BamHI GGATCC 2 cut(s) 560, 1781
BanII GRGCYC 2 cut(s) 1330, 1695
BarI GAAGNNNNNNTAC 2 cut(s) 867, 899
BbsI GAAGAC 1 cut(s) 1040
Bbv12I GWGCWC 3 cut(s) 906, 1330, 1510
BbvI GCAGC 5 cut(s) 729, 1057, 1409, 1453, 1481
BceAI ACGGC 2 cut(s) 40, 1786
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 1 cut(s) 1513
BcoDI GTCTC 6 cut(s) 43, 260, 337, 378, 511, 2195
BcuI ACTAGT 1 cut(s) 2057
BfaI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
BfmI CTRYAG 2 cut(s) 261, 1467
BfrI CTTAAG 1 cut(s) 1244
BfuAI ACCTGC 1 cut(s) 562
BglII AGATCT 1 cut(s) 1604
BisI GCNGC 7 cut(s) 743, 1071, 1233, 1398, 1467, 1470, 2130
BlpI GCTNAGC 1 cut(s) 1110
BlsI GCNGC 7 cut(s) 744, 1072, 1234, 1399, 1468, 1471, 2131
Bme1390I CCNGG 1 cut(s) 1513
Bme18I GGWCC 3 cut(s) 1598, 2088, 2135
BmeRI GACNNNNNGTC 2 cut(s) 56, 759
BmeT110I CYCGRG 1 cut(s) 1524
BmgBI CACGTC 1 cut(s) 830
BmgT120I GGNCC 5 cut(s) 1196, 1598, 1772, 2088, 2135
BmiI GGNNCC 5 cut(s) 562, 1783, 2090, 2128, 2136
BmrFI CCNGG 1 cut(s) 1513
BmrI ACTGGG 2 cut(s) 279, 2088
BmsI GCATC 9 cut(s) 64, 84, 154, 337, 700, 729, 820, 1024, 1034
BmtI GCTAGC 2 cut(s) 837, 1932
BmuI ACTGGG 2 cut(s) 279, 2088
BpiI GAAGAC 1 cut(s) 1040
BpmI CTGGAG 4 cut(s) 250, 357, 363, 981
Bpu1102I GCTNAGC 1 cut(s) 1110
Bpu14I TTCGAA 1 cut(s) 179
BpuEI CTTGAG 2 cut(s) 1410, 1741
BsaAI YACGTR 1 cut(s) 907
BsaBI GATNNNNATC 2 cut(s) 78, 224
BsaHI GRCGYC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 337
BsaJI CCNNGG 3 cut(s) 1119, 1511, 2130
BsaWI WCCGGW 1 cut(s) 2035
BsaXI ACNNNNNCTCC 6 cut(s) 397, 427, 484, 514, 864, 894
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 171, 557, 1058, 1502, 1570
Bse1I ACTGG 3 cut(s) 274, 1978, 2094
Bse21I CCTNAGG 1 cut(s) 1827
Bse3DI GCAATG 3 cut(s) 165, 589, 1168
Bse8I GATNNNNATC 2 cut(s) 78, 224
BseBI CCWGG 1 cut(s) 1513
BseDI CCNNGG 3 cut(s) 1119, 1511, 2130
BseGI GGATG 6 cut(s) 30, 744, 755, 1025, 1180, 2032
BseJI GATNNNNATC 2 cut(s) 78, 224
BseLI CCNNNNNNNGG 6 cut(s) 84, 171, 557, 1058, 1502, 1570
BseMI GCAATG 3 cut(s) 165, 589, 1168
BseMII CTCAG 6 cut(s) 137, 1101, 1538, 1680, 1818, 2115
BseNI ACTGG 3 cut(s) 274, 1978, 2094
BseRI GAGGAG 2 cut(s) 1139, 1306
BseSI GKGCMC 2 cut(s) 276, 542
BseXI GCAGC 5 cut(s) 729, 1057, 1409, 1453, 1481
BsgI GTGCAG 1 cut(s) 1108
Bsh1236I CGCG 1 cut(s) 2132
BshFI GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
BsiHKAI GWGCWC 3 cut(s) 906, 1330, 1510
BsiHKCI CYCGRG 1 cut(s) 1524
BsiSI CCGG 1 cut(s) 2036
BslFI GGGAC 4 cut(s) 290, 1144, 2090, 2148
BslI CCNNNNNNNGG 6 cut(s) 84, 171, 557, 1058, 1502, 1570
BsmAI GTCTC 6 cut(s) 43, 260, 337, 378, 511, 2195
BsmBI CGTCTC 1 cut(s) 43
BsmFI GGGAC 4 cut(s) 290, 1144, 2090, 2148
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
Bso31I GGTCTC 1 cut(s) 337
BsoBI CYCGRG 1 cut(s) 1524
Bsp119I TTCGAA 1 cut(s) 179
Bsp1286I GDGCHC 6 cut(s) 276, 542, 906, 1330, 1510, 1695
Bsp1407I TGTACA 4 cut(s) 293, 1450, 1702, 2023
Bsp143I GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
Bsp1720I GCTNAGC 1 cut(s) 1110
BspACI CCGC 5 cut(s) 601, 1232, 1932, 2130, 2132
BspANI GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
BspCNI CTCAG 6 cut(s) 136, 1102, 1539, 1679, 1819, 2114
BspFNI CGCG 1 cut(s) 2132
BspLI GGNNCC 5 cut(s) 562, 1783, 2090, 2128, 2136
BspMAI CTGCAG 1 cut(s) 1471
BspMI ACCTGC 1 cut(s) 562
BspOI GCTAGC 2 cut(s) 837, 1932
BspPI GGATC 7 cut(s) 211, 555, 568, 623, 1467, 1776, 1789
BspQI GCTCTTC 1 cut(s) 1671
BspT104I TTCGAA 1 cut(s) 179
BspTI CTTAAG 1 cut(s) 1244
BspTNI GGTCTC 1 cut(s) 337
BsrDI GCAATG 3 cut(s) 165, 589, 1168
BsrGI TGTACA 4 cut(s) 293, 1450, 1702, 2023
BsrI ACTGG 3 cut(s) 274, 1978, 2094
BssECI CCNNGG 3 cut(s) 1119, 1511, 2130
BssMI GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
BssNAI GTATAC 1 cut(s) 954
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 1119
Bst1107I GTATAC 1 cut(s) 954
Bst2UI CCWGG 1 cut(s) 1513
Bst4CI ACNGT 4 cut(s) 278, 1184, 2021, 2028
Bst6I CTCTTC 3 cut(s) 439, 870, 1671
BstACI GRCGYC 1 cut(s) 9
BstAFI CTTAAG 1 cut(s) 1244
BstAPI GCANNNNNTGC 1 cut(s) 546
BstAUI TGTACA 4 cut(s) 293, 1450, 1702, 2023
BstBAI YACGTR 1 cut(s) 907
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 8 cut(s) 188, 835, 839, 1116, 1159, 1897, 1910, 1930
BstDEI CTNAG 7 cut(s) 123, 887, 1110, 1547, 1666, 1827, 2101
BstDSI CCRYGG 1 cut(s) 2130
BstF5I GGATG 6 cut(s) 30, 744, 755, 1025, 1180, 2032
BstFNI CGCG 1 cut(s) 2132
BstHHI GCGC 2 cut(s) 1251, 2181
BstKTI GATC 8 cut(s) 206, 563, 631, 1462, 1568, 1607, 1665, 1784
BstMAI GTCTC 6 cut(s) 43, 260, 337, 378, 511, 2195
BstMBI GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
BstMWI GCNNNNNNNGC 8 cut(s) 347, 546, 598, 1115, 1397, 1905, 2126, 2187
BstNI CCWGG 1 cut(s) 1513
BstSCI CCNGG 1 cut(s) 1511
BstSFI CTRYAG 2 cut(s) 261, 1467
BstSLI GKGCMC 2 cut(s) 276, 542
BstUI CGCG 1 cut(s) 2132
BstV1I GCAGC 5 cut(s) 729, 1057, 1409, 1453, 1481
BstV2I GAAGAC 1 cut(s) 1040
BstX2I RGATCY 4 cut(s) 560, 1459, 1604, 1781
BstYI RGATCY 4 cut(s) 560, 1459, 1604, 1781
BstZ17I GTATAC 1 cut(s) 954
Bsu36I CCTNAGG 1 cut(s) 1827
BsuRI GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
BtgI CCRYGG 1 cut(s) 2130
BtrI CACGTC 1 cut(s) 830
BtsCI GGATG 6 cut(s) 30, 744, 755, 1025, 1180, 2032
BtsIMutI CAGTG 4 cut(s) 283, 749, 2033, 2101
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 8 cut(s) 188, 835, 839, 1116, 1159, 1897, 1910, 1930
CfoI GCGC 2 cut(s) 1251, 2181
Cfr13I GGNCC 5 cut(s) 1196, 1598, 1772, 2088, 2135
Cfr42I CCGCGG 1 cut(s) 2133
Csp6I GTAC 4 cut(s) 294, 1451, 1703, 2024
CviAII CATG 6 cut(s) 524, 851, 929, 1211, 1682, 2164
CviQI GTAC 4 cut(s) 294, 1451, 1703, 2024
DdeI CTNAG 7 cut(s) 123, 887, 1110, 1547, 1666, 1827, 2101
DpnI GATC 8 cut(s) 205, 562, 630, 1461, 1567, 1606, 1664, 1783
DpnII GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
DraI TTTAAA 1 cut(s) 654
DriI GACNNNNNGTC 2 cut(s) 56, 759
EaeI YGGCCR 2 cut(s) 1861, 1906
Eam1104I CTCTTC 3 cut(s) 439, 870, 1671
Eam1105I GACNNNNNGTC 2 cut(s) 56, 759
EarI CTCTTC 3 cut(s) 439, 870, 1671
Ecl136II GAGCTC 1 cut(s) 1328
Eco130I CCWWGG 1 cut(s) 1119
Eco24I GRGCYC 2 cut(s) 1330, 1695
Eco31I GGTCTC 1 cut(s) 337
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 3 cut(s) 1598, 2088, 2135
Eco53kI GAGCTC 1 cut(s) 1328
Eco57I CTGAAG 1 cut(s) 970
Eco81I CCTNAGG 1 cut(s) 1827
Eco88I CYCGRG 1 cut(s) 1524
EcoICRI GAGCTC 1 cut(s) 1328
EcoRI GAATTC 2 cut(s) 175, 1725
EcoRII CCWGG 1 cut(s) 1511
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 1119
EcoT22I ATGCAT 2 cut(s) 715, 813
EcoT38I GRGCYC 2 cut(s) 1330, 1695
ErhI CCWWGG 1 cut(s) 1119
Esp3I CGTCTC 1 cut(s) 43
FaeI CATG 6 cut(s) 527, 854, 932, 1214, 1685, 2167
FaqI GGGAC 4 cut(s) 290, 1144, 2090, 2148
FatI CATG 6 cut(s) 523, 850, 928, 1210, 1681, 2163
FauI CCCGC 1 cut(s) 2125
FauNDI CATATG 1 cut(s) 1272
FblI GTMKAC 2 cut(s) 953, 1978
Fnu4HI GCNGC 7 cut(s) 743, 1071, 1233, 1398, 1467, 1470, 2130
FokI GGATG 6 cut(s) 17, 742, 751, 1012, 1187, 2019
FriOI GRGCYC 2 cut(s) 1330, 1695
Fsp4HI GCNGC 7 cut(s) 743, 1071, 1233, 1398, 1467, 1470, 2130
FspBI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
GlaI GCGC 2 cut(s) 1250, 2180
GluI GCNGC 7 cut(s) 743, 1071, 1233, 1398, 1467, 1470, 2130
GsuI CTGGAG 4 cut(s) 250, 357, 363, 981
HaeIII GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
HapII CCGG 1 cut(s) 2036
HhaI GCGC 2 cut(s) 1251, 2181
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 6 cut(s) 527, 854, 932, 1214, 1685, 2167
Hin6I GCGC 2 cut(s) 1249, 2179
HinP1I GCGC 2 cut(s) 1249, 2179
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HindIII AAGCTT 3 cut(s) 248, 1347, 1711
HinfI GANTC 6 cut(s) 847, 1093, 1475, 1619, 1742, 2168
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 1 cut(s) 2036
HphI GGTGA 4 cut(s) 947, 1541, 1682, 1928
Hpy166II GTNNAC 7 cut(s) 793, 827, 954, 1451, 1979, 2017, 2024
Hpy8I GTNNAC 7 cut(s) 793, 827, 954, 1451, 1979, 2017, 2024
Hpy99I CGWCG 4 cut(s) 26, 62, 106, 1663
HpyAV CCTTC 3 cut(s) 108, 729, 1797
HpyCH4III ACNGT 4 cut(s) 278, 1184, 2021, 2028
HpyCH4IV ACGT 6 cut(s) 57, 823, 829, 906, 1065, 1658
HpyF10VI GCNNNNNNNGC 8 cut(s) 347, 546, 598, 1115, 1397, 1905, 2126, 2187
HpyF3I CTNAG 7 cut(s) 123, 887, 1110, 1547, 1666, 1827, 2101
HpySE526I ACGT 6 cut(s) 57, 823, 829, 906, 1065, 1658
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 6 cut(s) 527, 854, 932, 1214, 1685, 2167
HspAI GCGC 2 cut(s) 1249, 2179
KspAI GTTAAC 1 cut(s) 793
KspI CCGCGG 1 cut(s) 2133
Kzo9I GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
LguI GCTCTTC 1 cut(s) 1671
Lsp1109I GCAGC 5 cut(s) 729, 1057, 1409, 1453, 1481
LweI GCATC 9 cut(s) 64, 84, 154, 337, 700, 729, 820, 1024, 1034
MaeI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
MaeII ACGT 6 cut(s) 57, 823, 829, 906, 1065, 1658
MaeIII GTNAC 2 cut(s) 618, 1550
MalI GATC 8 cut(s) 205, 562, 630, 1461, 1567, 1606, 1664, 1783
MboI GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
MboII GAAGA 8 cut(s) 456, 515, 887, 1040, 1628, 1688, 1746, 1782
MflI RGATCY 4 cut(s) 560, 1459, 1604, 1781
MhlI GDGCHC 6 cut(s) 276, 542, 906, 1330, 1510, 1695
MlsI TGGCCA 2 cut(s) 1863, 1908
MluNI TGGCCA 2 cut(s) 1863, 1908
MlyI GAGTC 1 cut(s) 841
MmeI TCCRAC 5 cut(s) 224, 451, 743, 1152, 1855
Mox20I TGGCCA 2 cut(s) 1863, 1908
Mph1103I ATGCAT 2 cut(s) 715, 813
MroXI GAANNNNTTC 4 cut(s) 118, 879, 1536, 1741
MscI TGGCCA 2 cut(s) 1863, 1908
MseI TTAA 9 cut(s) 159, 194, 366, 380, 653, 792, 1245, 1715, 2186
MslI CAYNNNNRTG 3 cut(s) 933, 1869, 2196
Msp20I TGGCCA 2 cut(s) 1863, 1908
MspA1I CMGCKG 3 cut(s) 422, 1472, 2132
MspCI CTTAAG 1 cut(s) 1244
MspI CCGG 1 cut(s) 2036
MspR9I CCNGG 1 cut(s) 1513
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 1513
MvnI CGCG 1 cut(s) 2132
MwoI GCNNNNNNNGC 8 cut(s) 347, 546, 598, 1115, 1397, 1905, 2126, 2187
NdeI CATATG 1 cut(s) 1272
NdeII GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
NheI GCTAGC 2 cut(s) 833, 1928
NlaIII CATG 6 cut(s) 527, 854, 932, 1214, 1685, 2167
NlaIV GGNNCC 5 cut(s) 562, 1783, 2090, 2128, 2136
NmuCI GTSAC 2 cut(s) 618, 1550
NsiI ATGCAT 2 cut(s) 715, 813
NspV TTCGAA 1 cut(s) 179
PaeR7I CTCGAG 1 cut(s) 1524
PaqCI CACCTGC 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 1671
PcsI WCGNNNNNNNCGW 1 cut(s) 1444
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 4 cut(s) 118, 879, 1536, 1741
PfeI GAWTC 5 cut(s) 1093, 1475, 1619, 1742, 2168
PflMI CCANNNNNTGG 3 cut(s) 171, 557, 1502
PkrI GCNGC 7 cut(s) 744, 1072, 1234, 1399, 1468, 1471, 2131
PleI GAGTC 1 cut(s) 841
PpsI GAGTC 1 cut(s) 841
Ppu21I YACGTR 1 cut(s) 907
PsiI TTATAA 1 cut(s) 1293
Psp124BI GAGCTC 1 cut(s) 1330
Psp1406I AACGTT 1 cut(s) 1065
Psp6I CCWGG 1 cut(s) 1511
PspGI CCWGG 1 cut(s) 1511
PspN4I GGNNCC 5 cut(s) 562, 1783, 2090, 2128, 2136
PspPI GGNCC 5 cut(s) 1196, 1598, 1772, 2088, 2135
PspXI VCTCGAGB 1 cut(s) 1524
PstI CTGCAG 1 cut(s) 1471
PsuI RGATCY 4 cut(s) 560, 1459, 1604, 1781
PvuII CAGCTG 2 cut(s) 422, 1472
RsaI GTAC 4 cut(s) 295, 1452, 1704, 2025
RsaNI GTAC 4 cut(s) 294, 1451, 1703, 2024
RseI CAYNNNNRTG 3 cut(s) 933, 1869, 2196
SacI GAGCTC 1 cut(s) 1330
SacII CCGCGG 1 cut(s) 2133
SapI GCTCTTC 1 cut(s) 1671
SaqAI TTAA 9 cut(s) 159, 194, 366, 380, 653, 792, 1245, 1715, 2186
SatI GCNGC 7 cut(s) 743, 1071, 1233, 1398, 1467, 1470, 2130
Sau3AI GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
Sau96I GGNCC 5 cut(s) 1196, 1598, 1772, 2088, 2135
SchI GAGTC 1 cut(s) 841
ScrFI CCNGG 1 cut(s) 1513
SduI GDGCHC 6 cut(s) 276, 542, 906, 1330, 1510, 1695
SfaNI GCATC 9 cut(s) 64, 84, 154, 337, 700, 729, 820, 1024, 1034
SfcI CTRYAG 2 cut(s) 261, 1467
Sfr274I CTCGAG 1 cut(s) 1524
Sfr303I CCGCGG 1 cut(s) 2133
SfuI TTCGAA 1 cut(s) 179
SgrBI CCGCGG 1 cut(s) 2133
SinI GGWCC 3 cut(s) 1598, 2088, 2135
SlaI CTCGAG 1 cut(s) 1524
SmiMI CAYNNNNRTG 3 cut(s) 933, 1869, 2196
SmlI CTYRAG 4 cut(s) 1244, 1425, 1524, 1720
SmoI CTYRAG 4 cut(s) 1244, 1425, 1524, 1720
SpeI ACTAGT 1 cut(s) 2057
SsiI CCGC 5 cut(s) 601, 1232, 1932, 2130, 2132
SspMI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
SstI GAGCTC 1 cut(s) 1330
StyD4I CCNGG 1 cut(s) 1511
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 4 cut(s) 278, 1184, 2021, 2028
TaiI ACGT 6 cut(s) 60, 826, 832, 909, 1068, 1661
TaqI TCGA 8 cut(s) 60, 179, 358, 1008, 1525, 1661, 1689, 1732
TatI WGTACW 4 cut(s) 293, 1450, 1702, 2023
TauI GCSGC 2 cut(s) 1235, 2132
TfiI GAWTC 5 cut(s) 1093, 1475, 1619, 1742, 2168
Tru1I TTAA 9 cut(s) 159, 194, 366, 380, 653, 792, 1245, 1715, 2186
Tru9I TTAA 9 cut(s) 159, 194, 366, 380, 653, 792, 1245, 1715, 2186
TscAI CASTG 4 cut(s) 283, 756, 2033, 2101
TseFI GTSAC 2 cut(s) 618, 1550
TseI GCWGC 5 cut(s) 742, 1070, 1397, 1466, 1469
Tsp45I GTSAC 2 cut(s) 618, 1550
TspDTI ATGAA 7 cut(s) 932, 1259, 1689, 1718, 1821, 1859, 2019
TspGWI ACGGA 3 cut(s) 232, 784, 1895
TspRI CASTG 4 cut(s) 283, 756, 2033, 2101
Van91I CCANNNNNTGG 3 cut(s) 171, 557, 1502
Vha464I CTTAAG 1 cut(s) 1244
VpaK11BI GGWCC 3 cut(s) 1598, 2088, 2135
XapI RAATTY 7 cut(s) 175, 299, 643, 1385, 1420, 1725, 2082
XcmI CCANNNNNNNNNTGG 1 cut(s) 1871
XhoI CTCGAG 1 cut(s) 1524
XmiI GTMKAC 2 cut(s) 953, 1978
XmnI GAANNNNTTC 4 cut(s) 118, 879, 1536, 1741
XspI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
Zsp2I ATGCAT 2 cut(s) 715, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.