Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0007919

Physical Location & Seq

Genomic Coordinates Reverse (-)

9010240 .. 9020297

Gene Structure 10058 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000000996

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGGAGACCGTGCCTTTTCACGACGGCAACATCCACGACTTGATTGAGAGCCGATGCCTCGACGTTCGTCAAGATGTTAATCACAGCTTGCAGATGCACTCGTCATTGGTACGGCGGCTTTCTCAGGAGACGGAGTTGGAGGGGCATCAGGGTTGTGTTAATTCGATTGCTTGGAATTCTAGAGGTTCACTTTTAGTATCTGGATCAGACGACACACGGATAAATATTTGGAGCTACTCTGGTCGGAAGCTTTTGCATTCTATTGAGACTGGGCACTGTGCAAACATATTTTGTACAAAATTTGTCCCTGAAACTTCTGATGAGCTTGTTGTCTCTGGAGCTGGAGATGCAGAAGTTCGGTTATTTAATTTGTCTCAGTTAAGTGGGAGAGGACCTGAAGATAATGCAGTTCCTCCATCAGCTCTGTATCAATGTCACACAAGAAGAGTAAAAAAGTTAGCTGTTGAAGTTGGGAATCCCAATGTGGTATGGAGTGCAAGTGAAGATGGAACTTTGAGACAGCATGACTTTCGAGAGGGTACTTCTTGCCCTCCTGCTGGATCTGTTCATCAAGAATGTCGCAACATTTTACTTGACTTGCGGTCTGGAGCAAAGAAGTCACTAGCTGATCCCCCGAAACAAACATTTGCTCTAAAATCTTGTGATATCAGTTCCACTAGGCCTCATCTGCTATTAGTTGGTGGGAGTGATGCATTTGCGCGTTTATATGATAGAAGGATGCTGCCTCCTCTGACATCCTGCCGGAAAAGGACGTCTCCACCTCCTTGTGTTAACTATTTCTGTCCAATGCATCTCTCTGATCGTGGACGTACAAGCTTGCACTTAACCCACGTTACATTTAGCCCAGATGGAGAAGAGGTTCTACTTAGTTATAGTGGGGAGCATGTATATTTGATGAATGTAAATCATGCTTGTGGAAGTGCTGTGCAGTATACTTCAGGAGATGCTTCAAAGATAATGAGCTTCATACTGAATGGGCTAGAACTGCAGCAACCAGCATCCAGTGTCTTCAGAAGTCGTCTTCCTAAAAGACGCAAGGCCACTGCTATGCATGAAAAATGCAGTAAACTAACTCAAATTGCTGAGAAGTCCTTGGAGGAGGGGAATTTTTATCATGGCATTGAGGCATGCAATGAGGTGCTGGATGGATATGGTCGCGATATTGGGCCCATACTAAGGCATGATTGTCTCTGTACTCGTGCCGCATTGTTGCTGAAGCGTAATTGGAAAAACGATGCTCATATGGCTATAAGAGATTGCTATAATGCCCGGAAAATTGATAACTACTCTTTCAGAGCTCATTACCATATGTCTGAAGCTTTATCACAGTTGGCCAAACATAAAGAAGCTGTAGAATTTGCTATGGCAGCGCAATCTTTAGCTCCGTCGAATCCTGAGGTAACAGCACAATTGGAGAGTGTAAAAAGAAAGCTTGCTGCAGCTGAGTCTGAGAGAAATGGTAAGCCAAATGATGGGGCACCCAGGTCTGAACCTCGGGGTGGAAGAGTGCTATCCCTTAGTGACATACTTTACCGATCTGAGGGTAATAGTGAAGCTTCACAAGATGGTCCAAGATCTGAAAGAGAGGATTCTGATTATGACGAGGAATTGGAGTTGGACTTTGAAACGTCCATCTCAGGTGATGAAGAGCAAGATATCGAGCCCAATATTCTACATGGAAGTGTTAATGTGAGAATTCATCGAAGAAGTGATTCTTCTAGAGAAGTTGAAGGGGCAAATGGCTCTTGTGGATCACCTTCATCATCATCTCAAAATGAAAGACTCCCGTATCAGCCCGCGGCAGTTATTGATATGAAACAGAGATATGTCGGTCATTGTAATGTTGGAACTGACATAAAACAAGCCAGCTTTCTTGGCCAGAGAGGTGAATATGTTGCTAGTGGAAGTGATGATGGCAGGTGGTTTATCTGGGAGAAACGAACTGGCAGACTAATAAAAATGCTTCAAGGAGATGAAGCTGTTGTGAACTGTGTACAGTGCCATCCCTCCGATTGTGTAGTGGCAACTAGTGGGATTGACAGTACAATAAAGTTATGGACCCCAAGTGCTTCAGTCCCATCTATTGTAGCTGGTGGAGCCTCTGGACCAGAAACTGCTAACATTTCTAGCGTTTTGTCAGCTAATCAGCGCAGATTAAGCCATAATCGTGAAACCATCTTCCGTTCTGAAATTTTGGAGCATTTTCGAATGCATGAATTTACTGAAGGAAGCTTGCACCCGTTTGAGTGTGCTCAGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0019538 GO:0031974 GO:0031981 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

761

Amino Acids

84.01

Weight (kDa)

6.19

Isoelectric Point (pI)

63.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	30 - 125	3.7e-10	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	32 - 126	5.8e-07	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	38 - 181	1.6e-17	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	41 - 126	5.7e-13	WDR3 first beta-propeller domain
WD40	PF00400	43 - 78	1.1e-08	WD domain, G-beta repeat
EIF3I	PF24805	46 - 125	4.4e-06	EIF3I
Beta-prop_THOC3	PF25174	49 - 126	6.8e-11	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	612 - 694	1e-12	WDR5 beta-propeller domain
WD40_Prp19	PF24814	629 - 698	2.5e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	634 - 694	1.9e-06	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	635 - 694	8.2e-07	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007919)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10940 AT5G10940
fragaria_vesca	FvH4_5g35830 FvH4_5g35830 FvH4_5g35830
malus_domestica	MD08G1192800.v1.1 MD15G1381500.v1.1
prunus_persica	Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1
pyrus_communis	pycom08g16630 pycom15g34170
rosa_chinensis	RchiOBHm_Chr7g0237521
rosa_laevigata	RLG00000000996
rosa_multiflora	Rmu_ssc0000152.1_g000001
rosa_roxburghii	Rroxscaffold_3G00224060
rosa_rugosa	Rorug07G0303800 Rorug07G0303900
rosa_samantha	Rh7AG461500 Rh7BG431700 Rh7CG478700 Rh7DG447900
rosa_wichuraiana	Rw7G038190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1929
AatII	GACGTC	1 cut(s)	776
Acc36I	ACCTGC	1 cut(s)	1929
AccB1I	GGYRCC	1 cut(s)	1498
AccB7I	CCANNNNNTGG	1 cut(s)	1493
AccI	GTMKAC	1 cut(s)	953
AccII	CGCG	3 cut(s)	721, 1179, 1820
AciI	CCGC	5 cut(s)	115, 601, 1224, 1818, 1820
AclWI	GGATC	4 cut(s)	211, 568, 623, 1780
AcoI	YGGCCR	2 cut(s)	1353, 1897
AcsI	RAATTY	7 cut(s)	175, 299, 1126, 1376, 1716, 2211, 2237
AcuI	CTGAAG	7 cut(s)	417, 942, 1015, 1256, 1356, 2076, 2265
AcyI	GRCGYC	1 cut(s)	773
AfaI	GTAC	7 cut(s)	111, 295, 541, 832, 1216, 2016, 2065
AfiI	CCNNNNNNNGG	5 cut(s)	557, 1197, 1493, 1520, 1561
AgsI	TTSAA	5 cut(s)	467, 972, 1646, 1751, 1988
AhdI	GACNNNNNGTC	1 cut(s)	601
AhlI	ACTAGT	1 cut(s)	2048
AjnI	CCWGG	1 cut(s)	1502
AloI	GAACNNNNNNTCC	2 cut(s)	864, 896
Alw21I	GWGCWC	2 cut(s)	1321, 2275
Alw26I	GTCTC	7 cut(s)	122, 260, 337, 378, 511, 780, 1214
AlwI	GGATC	4 cut(s)	211, 568, 623, 1780
AlwNI	CAGNNNCTG	1 cut(s)	2135
Ama87I	CYCGRG	1 cut(s)	1515
AoxI	GGCC	5 cut(s)	680, 1059, 1187, 1353, 1897
ApaI	GGGCCC	1 cut(s)	1191
ApeKI	GCWGC	5 cut(s)	742, 1009, 1388, 1457, 1460
ApoI	RAATTY	7 cut(s)	175, 299, 1126, 1376, 1716, 2211, 2237
Asp700I	GAANNNNTTC	2 cut(s)	879, 1732
AspLEI	GCGC	3 cut(s)	721, 1393, 2172
AspS9I	GGNCC	6 cut(s)	392, 1187, 1188, 1589, 2079, 2126
AsuC2I	CCSGG	1 cut(s)	1291
AsuHPI	GGTGA	3 cut(s)	1673, 1767, 1919
AsuII	TTCGAA	1 cut(s)	2227
AvaI	CYCGRG	1 cut(s)	1515
AvaII	GGWCC	4 cut(s)	392, 1589, 2079, 2126
AxyI	CCTNAGG	1 cut(s)	1416
BaeGI	GKGCMC	3 cut(s)	276, 1191, 1501
BaeI	ACNNNNGTAYC	2 cut(s)	1793, 1826
BalI	TGGCCA	2 cut(s)	1355, 1899
BanI	GGYRCC	1 cut(s)	1498
BanII	GRGCYC	3 cut(s)	1191, 1321, 1686
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
BauI	CACGAG	1 cut(s)	1218
BbsI	GAAGAC	2 cut(s)	1021, 1034
Bbv12I	GWGCWC	2 cut(s)	1321, 2275
BbvI	GCAGC	5 cut(s)	729, 1021, 1400, 1444, 1472
BceAI	ACGGC	2 cut(s)	40, 128
BcgI	CGANNNNNNTGC	2 cut(s)	512, 546
BciT130I	CCWGG	1 cut(s)	1504
BcnI	CCSGG	1 cut(s)	1291
BcoDI	GTCTC	7 cut(s)	122, 260, 337, 378, 511, 780, 1214
BcuI	ACTAGT	1 cut(s)	2048
BfaI	CTAG	8 cut(s)	180, 623, 678, 1001, 1740, 1920, 2049, 2148
BfmI	CTRYAG	3 cut(s)	1007, 1371, 1458
BfuAI	ACCTGC	1 cut(s)	1929
BglII	AGATCT	1 cut(s)	1595
BisI	GCNGC	8 cut(s)	116, 743, 1010, 1224, 1389, 1458, 1461, 1821
BlsI	GCNGC	8 cut(s)	117, 744, 1011, 1225, 1390, 1459, 1462, 1822
Bme1390I	CCNGG	2 cut(s)	1291, 1504
Bme18I	GGWCC	4 cut(s)	392, 1589, 2079, 2126
BmeRI	GACNNNNNGTC	1 cut(s)	601
BmeT110I	CYCGRG	1 cut(s)	1515
BmgT120I	GGNCC	6 cut(s)	392, 1187, 1188, 1589, 2079, 2126
BmiI	GGNNCC	4 cut(s)	1189, 1500, 2081, 2119
BmrFI	CCNGG	2 cut(s)	1291, 1504
BmrI	ACTGGG	1 cut(s)	279
BmuI	ACTGGG	1 cut(s)	279
BoxI	GACNNNNGTC	1 cut(s)	66
BpiI	GAAGAC	2 cut(s)	1021, 1034
BpmI	CTGGAG	3 cut(s)	357, 363, 627
Bpu14I	TTCGAA	1 cut(s)	2227
BpuMI	CCSGG	1 cut(s)	1291
BsaBI	GATNNNNATC	1 cut(s)	78
BsaHI	GRCGYC	1 cut(s)	773
BsaJI	CCNNGG	4 cut(s)	1113, 1502, 1514, 1818
BsaXI	ACNNNNNCTCC	6 cut(s)	119, 149, 484, 514, 864, 894
Bsc4I	CCNNNNNNNGG	5 cut(s)	557, 1197, 1493, 1520, 1561
Bse1I	ACTGG	3 cut(s)	274, 1023, 1969
Bse21I	CCTNAGG	1 cut(s)	1416
Bse3DI	GCAATG	1 cut(s)	1159
Bse8I	GATNNNNATC	1 cut(s)	78
BseBI	CCWGG	1 cut(s)	1504
BseDI	CCNNGG	4 cut(s)	1113, 1502, 1514, 1818
BseGI	GGATG	6 cut(s)	30, 744, 755, 1019, 1171, 2023
BseJI	GATNNNNATC	1 cut(s)	78
BseLI	CCNNNNNNNGG	5 cut(s)	557, 1197, 1493, 1520, 1561
BseMI	GCAATG	1 cut(s)	1159
BseMII	CTCAG	8 cut(s)	137, 389, 1095, 1407, 1455, 1461, 1551, 1671
BseNI	ACTGG	3 cut(s)	274, 1023, 1969
BseRI	GAGGAG	2 cut(s)	738, 1133
BseSI	GKGCMC	3 cut(s)	276, 1191, 1501
BseXI	GCAGC	5 cut(s)	729, 1021, 1400, 1444, 1472
BsgI	GTGCAG	1 cut(s)	968
Bsh1236I	CGCG	3 cut(s)	721, 1179, 1820
BshFI	GGCC	5 cut(s)	682, 1061, 1189, 1355, 1899
BshNI	GGYRCC	1 cut(s)	1498
BsiHKAI	GWGCWC	2 cut(s)	1321, 2275
BsiHKCI	CYCGRG	1 cut(s)	1515
BsiSI	CCGG	2 cut(s)	763, 1291
BslFI	GGGAC	2 cut(s)	290, 2081
BslI	CCNNNNNNNGG	5 cut(s)	557, 1197, 1493, 1520, 1561
BsmAI	GTCTC	7 cut(s)	122, 260, 337, 378, 511, 780, 1214
BsmBI	CGTCTC	2 cut(s)	122, 780
BsmFI	GGGAC	2 cut(s)	290, 2081
BsmI	GAATGC	2 cut(s)	256, 2235
BsnI	GGCC	5 cut(s)	682, 1061, 1189, 1355, 1899
BsoBI	CYCGRG	1 cut(s)	1515
Bsp119I	TTCGAA	1 cut(s)	2227
Bsp120I	GGGCCC	1 cut(s)	1187
Bsp1286I	GDGCHC	6 cut(s)	276, 1191, 1321, 1501, 1686, 2275
Bsp1407I	TGTACA	2 cut(s)	293, 2014
Bsp143I	GATC	7 cut(s)	203, 560, 628, 820, 1556, 1595, 1772
Bsp68I	TCGCGA	1 cut(s)	1179
BspACI	CCGC	5 cut(s)	115, 601, 1224, 1818, 1820
BspANI	GGCC	5 cut(s)	682, 1061, 1189, 1355, 1899
BspCNI	CTCAG	8 cut(s)	136, 388, 1096, 1408, 1456, 1462, 1552, 1670
BspFNI	CGCG	3 cut(s)	721, 1179, 1820
BspLI	GGNNCC	4 cut(s)	1189, 1500, 2081, 2119
BspMAI	CTGCAG	2 cut(s)	1011, 1462
BspMI	ACCTGC	1 cut(s)	1929
BspPI	GGATC	4 cut(s)	211, 568, 623, 1780
BspQI	GCTCTTC	1 cut(s)	1662
BspT104I	TTCGAA	1 cut(s)	2227
BspT107I	GGYRCC	1 cut(s)	1498
BsrDI	GCAATG	1 cut(s)	1159
BsrGI	TGTACA	2 cut(s)	293, 2014
BsrI	ACTGG	3 cut(s)	274, 1023, 1969
BssECI	CCNNGG	4 cut(s)	1113, 1502, 1514, 1818
BssMI	GATC	7 cut(s)	203, 560, 628, 820, 1556, 1595, 1772
BssNAI	GTATAC	1 cut(s)	954
BssNI	GRCGYC	1 cut(s)	773
BssSI	CACGAG	1 cut(s)	1218
BssT1I	CCWWGG	1 cut(s)	1113
Bst1107I	GTATAC	1 cut(s)	954
Bst2BI	CACGAG	1 cut(s)	1218
Bst2UI	CCWGG	1 cut(s)	1504
Bst4CI	ACNGT	6 cut(s)	10, 278, 1350, 2012, 2019, 2063
Bst6I	CTCTTC	4 cut(s)	439, 870, 1519, 1662
BstACI	GRCGYC	1 cut(s)	773
BstAUI	TGTACA	2 cut(s)	293, 2014
BstBI	TTCGAA	1 cut(s)	2227
BstC8I	GCNNGC	7 cut(s)	89, 839, 1150, 1455, 1818, 1888, 2255
BstDSI	CCRYGG	1 cut(s)	1818
BstF5I	GGATG	6 cut(s)	30, 744, 755, 1019, 1171, 2023
BstFNI	CGCG	3 cut(s)	721, 1179, 1820
BstHHI	GCGC	3 cut(s)	721, 1393, 2172
BstKTI	GATC	7 cut(s)	206, 563, 631, 823, 1559, 1598, 1775
BstMAI	GTCTC	7 cut(s)	122, 260, 337, 378, 511, 780, 1214
BstMBI	GATC	7 cut(s)	203, 560, 628, 820, 1556, 1595, 1772
BstMWI	GCNNNNNNNGC	8 cut(s)	347, 688, 1006, 1265, 1388, 1896, 2117, 2178
BstNI	CCWGG	1 cut(s)	1504
BstNSI	RCATGY	2 cut(s)	908, 1152
BstPAI	GACNNNNGTC	1 cut(s)	66
BstSCI	CCNGG	2 cut(s)	1289, 1502
BstSFI	CTRYAG	3 cut(s)	1007, 1371, 1458
BstSLI	GKGCMC	3 cut(s)	276, 1191, 1501
BstUI	CGCG	3 cut(s)	721, 1179, 1820
BstV1I	GCAGC	5 cut(s)	729, 1021, 1400, 1444, 1472
BstV2I	GAAGAC	2 cut(s)	1021, 1034
BstX2I	RGATCY	2 cut(s)	560, 1595
BstYI	RGATCY	2 cut(s)	560, 1595
BstZ17I	GTATAC	1 cut(s)	954
Bsu36I	CCTNAGG	1 cut(s)	1416
BsuRI	GGCC	5 cut(s)	682, 1061, 1189, 1355, 1899
BtgI	CCRYGG	1 cut(s)	1818
BtsCI	GGATG	6 cut(s)	30, 744, 755, 1019, 1171, 2023
BtsI	GCAGTG	1 cut(s)	1062
BtsIMutI	CAGTG	4 cut(s)	274, 1030, 1062, 2024
BtuMI	TCGCGA	1 cut(s)	1179
BveI	ACCTGC	1 cut(s)	1929
Cac8I	GCNNGC	7 cut(s)	89, 839, 1150, 1455, 1818, 1888, 2255
CaiI	CAGNNNCTG	1 cut(s)	2135
CfoI	GCGC	3 cut(s)	721, 1393, 2172
Cfr13I	GGNCC	6 cut(s)	392, 1187, 1188, 1589, 2079, 2126
Cfr42I	CCGCGG	1 cut(s)	1821
CseI	GACGC	1 cut(s)	1062
Csp6I	GTAC	7 cut(s)	110, 294, 540, 831, 1215, 2015, 2064
CviAII	CATG	9 cut(s)	524, 905, 929, 1073, 1136, 1149, 1202, 1697, 2234
CviQI	GTAC	7 cut(s)	110, 294, 540, 831, 1215, 2015, 2064
DpnI	GATC	7 cut(s)	205, 562, 630, 822, 1558, 1597, 1774
DpnII	GATC	7 cut(s)	203, 560, 628, 820, 1556, 1595, 1772
DriI	GACNNNNNGTC	1 cut(s)	601
EaeI	YGGCCR	2 cut(s)	1353, 1897
Eam1104I	CTCTTC	4 cut(s)	439, 870, 1519, 1662
Eam1105I	GACNNNNNGTC	1 cut(s)	601
EarI	CTCTTC	4 cut(s)	439, 870, 1519, 1662
Ecl136II	GAGCTC	1 cut(s)	1319
Eco130I	CCWWGG	1 cut(s)	1113
Eco147I	AGGCCT	1 cut(s)	682
Eco24I	GRGCYC	3 cut(s)	1191, 1321, 1686
Eco32I	GATATC	2 cut(s)	667, 1678
Eco47I	GGWCC	4 cut(s)	392, 1589, 2079, 2126
Eco53kI	GAGCTC	1 cut(s)	1319
Eco57I	CTGAAG	7 cut(s)	417, 942, 1015, 1256, 1356, 2076, 2265
Eco81I	CCTNAGG	1 cut(s)	1416
Eco88I	CYCGRG	1 cut(s)	1515
EcoICRI	GAGCTC	1 cut(s)	1319
EcoO109I	RGGNCCY	1 cut(s)	392
EcoRI	GAATTC	2 cut(s)	175, 1716
EcoRII	CCWGG	1 cut(s)	1502
EcoRV	GATATC	2 cut(s)	667, 1678
EcoT14I	CCWWGG	1 cut(s)	1113
EcoT22I	ATGCAT	4 cut(s)	715, 813, 1074, 2235
EcoT38I	GRGCYC	3 cut(s)	1191, 1321, 1686
ErhI	CCWWGG	1 cut(s)	1113
Esp3I	CGTCTC	2 cut(s)	122, 780
FaeI	CATG	9 cut(s)	527, 908, 932, 1076, 1139, 1152, 1205, 1700, 2237
FalI	AAGNNNNNCTT	2 cut(s)	1720, 1752
FaqI	GGGAC	2 cut(s)	290, 2081
FatI	CATG	9 cut(s)	523, 904, 928, 1072, 1135, 1148, 1201, 1696, 2233
FauI	CCCGC	1 cut(s)	1825
FauNDI	CATATG	2 cut(s)	1263, 1329
FblI	GTMKAC	1 cut(s)	953
Fnu4HI	GCNGC	8 cut(s)	116, 743, 1010, 1224, 1389, 1458, 1461, 1821
FokI	GGATG	6 cut(s)	17, 742, 751, 1006, 1178, 2010
FriOI	GRGCYC	3 cut(s)	1191, 1321, 1686
Fsp4HI	GCNGC	8 cut(s)	116, 743, 1010, 1224, 1389, 1458, 1461, 1821
FspBI	CTAG	8 cut(s)	180, 623, 678, 1001, 1740, 1920, 2049, 2148
GlaI	GCGC	3 cut(s)	720, 1392, 2171
GluI	GCNGC	8 cut(s)	116, 743, 1010, 1224, 1389, 1458, 1461, 1821
GsuI	CTGGAG	3 cut(s)	357, 363, 627
HaeIII	GGCC	5 cut(s)	682, 1061, 1189, 1355, 1899
HapII	CCGG	2 cut(s)	763, 1291
HgaI	GACGC	1 cut(s)	1062
HhaI	GCGC	3 cut(s)	721, 1393, 2172
Hin1I	GRCGYC	1 cut(s)	773
Hin1II	CATG	9 cut(s)	527, 908, 932, 1076, 1139, 1152, 1205, 1700, 2237
Hin6I	GCGC	3 cut(s)	719, 1391, 2170
HinP1I	GCGC	3 cut(s)	719, 1391, 2170
HincII	GTYRAC	1 cut(s)	793
HindII	GTYRAC	1 cut(s)	793
HindIII	AAGCTT	6 cut(s)	248, 835, 1338, 1451, 1575, 2251
HinfI	GANTC	6 cut(s)	475, 1411, 1466, 1610, 1733, 1803
HpaI	GTTAAC	1 cut(s)	793
HpaII	CCGG	2 cut(s)	763, 1291
HphI	GGTGA	3 cut(s)	1673, 1767, 1919
Hpy166II	GTNNAC	7 cut(s)	188, 793, 827, 954, 1088, 2008, 2015
Hpy8I	GTNNAC	7 cut(s)	188, 793, 827, 954, 1088, 2008, 2015
Hpy99I	CGWCG	3 cut(s)	26, 65, 1411
HpyAV	CCTTC	4 cut(s)	729, 1745, 1788, 2240
HpyCH4III	ACNGT	6 cut(s)	10, 278, 1350, 2012, 2019, 2063
HpyCH4IV	ACGT	5 cut(s)	63, 773, 829, 852, 1649
HpyF10VI	GCNNNNNNNGC	8 cut(s)	347, 688, 1006, 1265, 1388, 1896, 2117, 2178
HpySE526I	ACGT	5 cut(s)	63, 773, 829, 852, 1649
Hsp92I	GRCGYC	1 cut(s)	773
Hsp92II	CATG	9 cut(s)	527, 908, 932, 1076, 1139, 1152, 1205, 1700, 2237
HspAI	GCGC	3 cut(s)	719, 1391, 2170
KspAI	GTTAAC	1 cut(s)	793
KspI	CCGCGG	1 cut(s)	1821
Kzo9I	GATC	7 cut(s)	203, 560, 628, 820, 1556, 1595, 1772
LguI	GCTCTTC	1 cut(s)	1662
LmnI	GCTCC	7 cut(s)	231, 338, 608, 901, 1408, 2117, 2218
Lsp1109I	GCAGC	5 cut(s)	729, 1021, 1400, 1444, 1472
MaeI	CTAG	8 cut(s)	180, 623, 678, 1001, 1740, 1920, 2049, 2148
MaeII	ACGT	5 cut(s)	63, 773, 829, 852, 1649
MaeIII	GTNAC	5 cut(s)	434, 618, 853, 1420, 1541
MalI	GATC	7 cut(s)	205, 562, 630, 822, 1558, 1597, 1774
MboI	GATC	7 cut(s)	203, 560, 628, 820, 1556, 1595, 1772
MfeI	CAATTG	1 cut(s)	1430
MflI	RGATCY	2 cut(s)	560, 1595
MhlI	GDGCHC	6 cut(s)	276, 1191, 1321, 1501, 1686, 2275
MlsI	TGGCCA	2 cut(s)	1355, 1899
MluNI	TGGCCA	2 cut(s)	1355, 1899
MlyI	GAGTC	2 cut(s)	1475, 1797
MmeI	TCCRAC	4 cut(s)	117, 224, 1617, 1846
Mox20I	TGGCCA	2 cut(s)	1355, 1899
Mph1103I	ATGCAT	4 cut(s)	715, 813, 1074, 2235
MroXI	GAANNNNTTC	2 cut(s)	879, 1732
MscI	TGGCCA	2 cut(s)	1355, 1899
MseI	TTAA	8 cut(s)	78, 159, 366, 380, 792, 845, 1707, 2177
MslI	CAYNNNNRTG	5 cut(s)	933, 1067, 1701, 1860, 2187
Msp20I	TGGCCA	2 cut(s)	1355, 1899
MspA1I	CMGCKG	2 cut(s)	1463, 1820
MspI	CCGG	2 cut(s)	763, 1291
MspR9I	CCNGG	2 cut(s)	1291, 1504
MunI	CAATTG	1 cut(s)	1430
Mva1269I	GAATGC	2 cut(s)	256, 2235
MvaI	CCWGG	1 cut(s)	1504
MvnI	CGCG	3 cut(s)	721, 1179, 1820
MwoI	GCNNNNNNNGC	8 cut(s)	347, 688, 1006, 1265, 1388, 1896, 2117, 2178
NciI	CCSGG	1 cut(s)	1291
NdeI	CATATG	2 cut(s)	1263, 1329
NdeII	GATC	7 cut(s)	203, 560, 628, 820, 1556, 1595, 1772
NlaIII	CATG	9 cut(s)	527, 908, 932, 1076, 1139, 1152, 1205, 1700, 2237
NlaIV	GGNNCC	4 cut(s)	1189, 1500, 2081, 2119
NmuCI	GTSAC	3 cut(s)	434, 618, 1541
NruI	TCGCGA	1 cut(s)	1179
NsiI	ATGCAT	4 cut(s)	715, 813, 1074, 2235
NspI	RCATGY	2 cut(s)	908, 1152
NspV	TTCGAA	1 cut(s)	2227
PaeI	GCATGC	1 cut(s)	1152
PaqCI	CACCTGC	1 cut(s)	1929
PceI	AGGCCT	1 cut(s)	682
PciSI	GCTCTTC	1 cut(s)	1662
PctI	GAATGC	2 cut(s)	256, 2235
PdmI	GAANNNNTTC	2 cut(s)	879, 1732
PfeI	GAWTC	4 cut(s)	475, 1411, 1610, 1733
PflMI	CCANNNNNTGG	1 cut(s)	1493
PkrI	GCNGC	8 cut(s)	117, 744, 1011, 1225, 1390, 1459, 1462, 1822
PleI	GAGTC	2 cut(s)	1474, 1797
PpsI	GAGTC	2 cut(s)	1474, 1797
PpuMI	RGGWCCY	1 cut(s)	392
PshAI	GACNNNNGTC	1 cut(s)	66
Psp124BI	GAGCTC	1 cut(s)	1321
Psp5II	RGGWCCY	1 cut(s)	392
Psp6I	CCWGG	1 cut(s)	1502
PspGI	CCWGG	1 cut(s)	1502
PspN4I	GGNNCC	4 cut(s)	1189, 1500, 2081, 2119
PspOMI	GGGCCC	1 cut(s)	1187
PspPI	GGNCC	6 cut(s)	392, 1187, 1188, 1589, 2079, 2126
PspPPI	RGGWCCY	1 cut(s)	392
PstI	CTGCAG	2 cut(s)	1011, 1462
PstNI	CAGNNNCTG	1 cut(s)	2135
PsuI	RGATCY	2 cut(s)	560, 1595
PvuII	CAGCTG	1 cut(s)	1463
RruI	TCGCGA	1 cut(s)	1179
RsaI	GTAC	7 cut(s)	111, 295, 541, 832, 1216, 2016, 2065
RsaNI	GTAC	7 cut(s)	110, 294, 540, 831, 1215, 2015, 2064
RseI	CAYNNNNRTG	5 cut(s)	933, 1067, 1701, 1860, 2187
SacI	GAGCTC	1 cut(s)	1321
SacII	CCGCGG	1 cut(s)	1821
SapI	GCTCTTC	1 cut(s)	1662
SaqAI	TTAA	8 cut(s)	78, 159, 366, 380, 792, 845, 1707, 2177
SatI	GCNGC	8 cut(s)	116, 743, 1010, 1224, 1389, 1458, 1461, 1821
Sau3AI	GATC	7 cut(s)	203, 560, 628, 820, 1556, 1595, 1772
Sau96I	GGNCC	6 cut(s)	392, 1187, 1188, 1589, 2079, 2126
SchI	GAGTC	2 cut(s)	1475, 1797
ScrFI	CCNGG	2 cut(s)	1291, 1504
SduI	GDGCHC	6 cut(s)	276, 1191, 1321, 1501, 1686, 2275
SfcI	CTRYAG	3 cut(s)	1007, 1371, 1458
Sfr303I	CCGCGG	1 cut(s)	1821
SfuI	TTCGAA	1 cut(s)	2227
SgrBI	CCGCGG	1 cut(s)	1821
SinI	GGWCC	4 cut(s)	392, 1589, 2079, 2126
SmiMI	CAYNNNNRTG	5 cut(s)	933, 1067, 1701, 1860, 2187
SpeI	ACTAGT	1 cut(s)	2048
SphI	GCATGC	1 cut(s)	1152
SseBI	AGGCCT	1 cut(s)	682
SsiI	CCGC	5 cut(s)	115, 601, 1224, 1818, 1820
SspI	AATATT	2 cut(s)	226, 1690
SspMI	CTAG	8 cut(s)	180, 623, 678, 1001, 1740, 1920, 2049, 2148
SstI	GAGCTC	1 cut(s)	1321
StuI	AGGCCT	1 cut(s)	682
StyD4I	CCNGG	2 cut(s)	1289, 1502
StyI	CCWWGG	1 cut(s)	1113
TaaI	ACNGT	6 cut(s)	10, 278, 1350, 2012, 2019, 2063
TaiI	ACGT	5 cut(s)	66, 776, 832, 855, 1652
TaqI	TCGA	7 cut(s)	60, 164, 532, 1409, 1680, 1723, 2227
TaqII	GACCGA	1 cut(s)	1841
TatI	WGTACW	4 cut(s)	293, 1214, 2014, 2063
TauI	GCSGC	3 cut(s)	118, 1226, 1823
TfiI	GAWTC	4 cut(s)	475, 1411, 1610, 1733
Tru1I	TTAA	8 cut(s)	78, 159, 366, 380, 792, 845, 1707, 2177
Tru9I	TTAA	8 cut(s)	78, 159, 366, 380, 792, 845, 1707, 2177
TscAI	CASTG	4 cut(s)	281, 1030, 1069, 2024
TseFI	GTSAC	3 cut(s)	434, 618, 1541
TseI	GCWGC	5 cut(s)	742, 1009, 1388, 1457, 1460
Tsp45I	GTSAC	3 cut(s)	434, 618, 1541
TspGWI	ACGGA	4 cut(s)	146, 232, 1395, 2192
TspRI	CASTG	4 cut(s)	281, 1030, 1069, 2024
Van91I	CCANNNNNTGG	1 cut(s)	1493
VpaK11BI	GGWCC	4 cut(s)	392, 1589, 2079, 2126
XapI	RAATTY	7 cut(s)	175, 299, 1126, 1376, 1716, 2211, 2237
XbaI	TCTAGA	2 cut(s)	179, 1739
XceI	RCATGY	2 cut(s)	908, 1152
XmiI	GTMKAC	1 cut(s)	953
XmnI	GAANNNNTTC	2 cut(s)	879, 1732
XspI	CTAG	8 cut(s)	180, 623, 678, 1001, 1740, 1920, 2049, 2148
ZraI	GACGTC	1 cut(s)	774
Zsp2I	ATGCAT	4 cut(s)	715, 813, 1074, 2235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.