FvH4_5g35910

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012089
Physical Location & Seq
Reverse (-)
26323903 .. 26328368
4466 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35910.t1

Sequence Viewer

Length: 891 bp
ATGAGTGAGCATCATCAGCCAGCCGGCGACACAAAGCCTCCTACTCACTCCCAAGACTACGCGCCTTACCCTAAGCTCGATCCAAACGACGTCGCTCCGCCTCCCCACTCCGAAACCTGGACCTCCGTCTCCATCGCCTCAGAACAGCCGCCGAAGTCGGAGTCTCCGCCGTCGGCCCCGGCACCGCCGCCCGTCGCGACTCCTGAAGTACACCCCGAGGCTCGCGCACCGCCGATCGCCGCAGAAGACGCCGCCACTACCATGCCTATGGAGTCCAATCCTTACGTCAGCAATCCGGCGCAGCCTCCCGCTTCGTCCGTCAAGAATACGGTGGATTCAGTGAAAGGTGTTCTTGGAAAATGGGGAAAGAAGGCTGTGGAGACTACCAAGAAGGGTCAGGATCTTGCGGGCAACATGTGGCAACACTTGAAAACAGGACCCAGTTTTGCTGACGCTGCTGTGGGAAGAATTGCTCAGAGTGCAAAAGTGATTGCTGAAGGTGGTTATGAGAAGATCTTTCAGAAAAATTTTGAGACAGTCCCGGACGAGCAACTTCTGAAGACCTTTGCTTGCTACCTGTCCACATCTGCTGGCCCCGTGATGGGAGTTATATATTTGTCAACAGCAAAGCTTGCATTTTGCAGTGATGATCCCCTTTCATACAAAAATGGTGATCAGACAGCGTGGAGCTATTATAAGGTGGTTATTCCATTACATCAGCTTAAGGCAGTGAACCCATCAACGAGCAAAGTCAAAGCAGCTGAGAAATACATCCAGGTCATCTCTGTGGACAACCACGAGTTCTGGTTTATGAGCTTTGTGAACTATGATAGTGCTGTGAAACACCTTCAAGAAGCATTGCAGCCCCACCCTCAGCCCCACAGCTTGTAA

Protein Analysis

297

Amino Acids

32.06

Weight (kDa)

6.11

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 172 - 288 3.3e-20 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28200 AT1G28200
fragaria_vesca FvH4_5g35910
malus_domestica MD08G1192100.v1.1 MD15G1380800.v1.1
prunus_persica Prupe.1G527000_v2.0.a1
pyrus_communis pycom08g16590 pycom15g34130
rosa_chinensis RchiOBHm_Chr7g0237641
rosa_laevigata RLG00000000979
rosa_multiflora Rmu_sc0007826.1_g000010
rosa_roxburghii Rroxscaffold_3G00223910
rosa_rugosa Rorug07G0305800 Rorug07G0305900
rosa_samantha Rh7AG462600 Rh7BG432600 Rh7CG479400 Rh7DG448400
rosa_wichuraiana Rw7G038270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 181
AccII CGCG 3 cut(s) 62, 197, 225
AclWI GGATC 3 cut(s) 74, 408, 644
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 3 cut(s) 225, 516, 578
AcyI GRCGYC 2 cut(s) 90, 249
AfaI GTAC 1 cut(s) 210
AfiI CCNNNNNNNGG 3 cut(s) 117, 601, 602
AflII CTTAAG 1 cut(s) 722
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 2 cut(s) 430, 851
AjnI CCWGG 2 cut(s) 116, 774
AluBI AGCT 7 cut(s) 76, 631, 690, 721, 761, 816, 885
AluI AGCT 7 cut(s) 76, 631, 690, 721, 761, 816, 885
Alw26I GTCTC 4 cut(s) 133, 168, 374, 527
AlwI GGATC 3 cut(s) 74, 408, 644
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 2 cut(s) 174, 592
ApeKI GCWGC 4 cut(s) 301, 455, 758, 862
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspLEI GCGC 3 cut(s) 64, 227, 301
AspS9I GGNCC 4 cut(s) 120, 175, 437, 593
AsuC2I CCSGG 2 cut(s) 179, 542
AsuHPI GGTGA 1 cut(s) 683
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 2 cut(s) 120, 437
BanI GGYRCC 1 cut(s) 181
BauI CACGAG 1 cut(s) 797
BbsI GAAGAC 2 cut(s) 252, 566
BbvCI CCTCAGC 1 cut(s) 873
BbvI GCAGC 4 cut(s) 313, 442, 770, 874
BccI CCATC 3 cut(s) 140, 595, 745
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 2 cut(s) 118, 776
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 2 cut(s) 179, 542
BcoDI GTCTC 4 cut(s) 133, 168, 374, 527
BfrI CTTAAG 1 cut(s) 722
BglII AGATCT 1 cut(s) 513
BisI GCNGC 8 cut(s) 149, 188, 240, 252, 302, 456, 759, 863
BlsI GCNGC 8 cut(s) 150, 189, 241, 253, 303, 457, 760, 864
Bme1390I CCNGG 4 cut(s) 118, 179, 542, 776
Bme18I GGWCC 2 cut(s) 120, 437
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 4 cut(s) 120, 175, 437, 593
BmiI GGNNCC 4 cut(s) 177, 183, 439, 595
BmrFI CCNGG 4 cut(s) 118, 179, 542, 776
BmrI ACTGGG 1 cut(s) 435
BmsI GCATC 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 435
BoxI GACNNNNGTC 1 cut(s) 125
BpiI GAAGAC 2 cut(s) 252, 566
Bpu10I CCTNAGC 2 cut(s) 72, 873
BpuMI CCSGG 2 cut(s) 179, 542
BsaHI GRCGYC 2 cut(s) 90, 249
BsaJI CCNNGG 2 cut(s) 177, 216
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 601, 602
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 441
Bse3DI GCAATG 1 cut(s) 857
BseBI CCWGG 2 cut(s) 118, 776
BseDI CCNNGG 2 cut(s) 177, 216
BseGI GGATG 1 cut(s) 771
BseLI CCNNNNNNNGG 3 cut(s) 117, 601, 602
BseMI GCAATG 1 cut(s) 857
BseMII CTCAG 4 cut(s) 153, 488, 753, 887
BseNI ACTGG 1 cut(s) 441
BseXI GCAGC 4 cut(s) 313, 442, 770, 874
Bsh1236I CGCG 3 cut(s) 62, 197, 225
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 2 cut(s) 176, 594
BshNI GGYRCC 1 cut(s) 181
BsiEI CGRYCG 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 4 cut(s) 24, 179, 296, 542
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 3 cut(s) 117, 601, 602
BsmAI GTCTC 4 cut(s) 133, 168, 374, 527
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 2 cut(s) 176, 594
BsoBI CYCGRG 1 cut(s) 215
Bsp143I GATC 6 cut(s) 79, 234, 400, 513, 649, 673
Bsp68I TCGCGA 1 cut(s) 197
BspANI GGCC 2 cut(s) 176, 594
BspCNI CTCAG 4 cut(s) 152, 487, 754, 886
BspFNI CGCG 3 cut(s) 62, 197, 225
BspLI GGNNCC 4 cut(s) 177, 183, 439, 595
BspPI GGATC 3 cut(s) 74, 408, 644
BspT107I GGYRCC 1 cut(s) 181
BspTI CTTAAG 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 857
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 1 cut(s) 441
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 177, 216
BssMI GATC 6 cut(s) 79, 234, 400, 513, 649, 673
BssNI GRCGYC 2 cut(s) 90, 249
BssSI CACGAG 1 cut(s) 797
Bst2BI CACGAG 1 cut(s) 797
Bst2UI CCWGG 2 cut(s) 118, 776
Bst4CI ACNGT 2 cut(s) 331, 538
BstACI GRCGYC 2 cut(s) 90, 249
BstAFI CTTAAG 1 cut(s) 722
BstAPI GCANNNNNTGC 1 cut(s) 632
BstC8I GCNNGC 7 cut(s) 21, 25, 223, 409, 571, 592, 633
BstDEI CTNAG 5 cut(s) 72, 139, 474, 762, 873
BstF5I GGATG 1 cut(s) 771
BstFNI CGCG 3 cut(s) 62, 197, 225
BstHHI GCGC 3 cut(s) 64, 227, 301
BstKTI GATC 6 cut(s) 82, 237, 403, 516, 652, 676
BstMAI GTCTC 4 cut(s) 133, 168, 374, 527
BstMBI GATC 6 cut(s) 79, 234, 400, 513, 649, 673
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 5 cut(s) 16, 248, 455, 479, 632
BstNI CCWGG 2 cut(s) 118, 776
BstNSI RCATGY 1 cut(s) 418
BstPAI GACNNNNGTC 1 cut(s) 125
BstSCI CCNGG 4 cut(s) 116, 177, 540, 774
BstUI CGCG 3 cut(s) 62, 197, 225
BstV1I GCAGC 4 cut(s) 313, 442, 770, 874
BstV2I GAAGAC 2 cut(s) 252, 566
BstX2I RGATCY 2 cut(s) 400, 513
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 2 cut(s) 400, 513
BsuRI GGCC 2 cut(s) 176, 594
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 771
BtsI GCAGTG 2 cut(s) 649, 735
BtsIMutI CAGTG 3 cut(s) 345, 649, 735
BtuMI TCGCGA 1 cut(s) 197
Cac8I GCNNGC 7 cut(s) 21, 25, 223, 409, 571, 592, 633
CfoI GCGC 3 cut(s) 64, 227, 301
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 4 cut(s) 120, 175, 437, 593
CseI GACGC 2 cut(s) 257, 461
Csp6I GTAC 1 cut(s) 209
CviAII CATG 2 cut(s) 262, 415
CviQI GTAC 1 cut(s) 209
DdeI CTNAG 5 cut(s) 72, 139, 474, 762, 873
DpnI GATC 6 cut(s) 81, 236, 402, 515, 651, 675
DpnII GATC 6 cut(s) 79, 234, 400, 513, 649, 673
EciI GGCGGA 2 cut(s) 87, 156
Eco47I GGWCC 2 cut(s) 120, 437
Eco57I CTGAAG 3 cut(s) 225, 516, 578
Eco88I CYCGRG 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 116, 774
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 2 cut(s) 265, 418
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 1 cut(s) 524
FatI CATG 2 cut(s) 261, 414
FauI CCCGC 2 cut(s) 316, 400
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 8 cut(s) 149, 188, 240, 252, 302, 456, 759, 863
FokI GGATG 1 cut(s) 758
Fsp4HI GCNGC 8 cut(s) 149, 188, 240, 252, 302, 456, 759, 863
GlaI GCGC 3 cut(s) 63, 226, 300
GluI GCNGC 8 cut(s) 149, 188, 240, 252, 302, 456, 759, 863
HaeIII GGCC 2 cut(s) 176, 594
HapII CCGG 4 cut(s) 24, 179, 296, 542
HgaI GACGC 2 cut(s) 257, 461
HhaI GCGC 3 cut(s) 64, 227, 301
Hin1I GRCGYC 2 cut(s) 90, 249
Hin1II CATG 2 cut(s) 265, 418
Hin6I GCGC 3 cut(s) 62, 225, 299
HinP1I GCGC 3 cut(s) 62, 225, 299
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 4 cut(s) 161, 199, 272, 335
HpaII CCGG 4 cut(s) 24, 179, 296, 542
HphI GGTGA 1 cut(s) 683
Hpy166II GTNNAC 6 cut(s) 211, 582, 621, 733, 790, 823
Hpy188I TCNGA 7 cut(s) 112, 142, 160, 477, 522, 558, 678
Hpy188III TCNNGA 5 cut(s) 196, 203, 322, 398, 851
Hpy8I GTNNAC 6 cut(s) 211, 582, 621, 733, 790, 823
Hpy99I CGWCG 4 cut(s) 92, 95, 175, 197
HpyAV CCTTC 4 cut(s) 364, 385, 491, 857
HpyCH4III ACNGT 2 cut(s) 331, 538
HpyCH4IV ACGT 2 cut(s) 90, 285
HpyCH4V TGCA 4 cut(s) 482, 635, 642, 862
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 248, 455, 479, 632
HpyF3I CTNAG 5 cut(s) 72, 139, 474, 762, 873
HpySE526I ACGT 2 cut(s) 90, 285
Hsp92I GRCGYC 2 cut(s) 90, 249
Hsp92II CATG 2 cut(s) 265, 418
HspAI GCGC 3 cut(s) 62, 225, 299
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 6 cut(s) 79, 234, 400, 513, 649, 673
LmnI GCTCC 2 cut(s) 100, 687
Lsp1109I GCAGC 4 cut(s) 313, 442, 770, 874
LweI GCATC 1 cut(s) 19
MaeII ACGT 2 cut(s) 90, 285
MalI GATC 6 cut(s) 81, 236, 402, 515, 651, 675
MboI GATC 6 cut(s) 79, 234, 400, 513, 649, 673
MboII GAAGA 4 cut(s) 257, 477, 523, 571
MflI RGATCY 2 cut(s) 400, 513
MluCI AATT 2 cut(s) 468, 526
MlyI GAGTC 3 cut(s) 170, 193, 281
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 7 cut(s) 48, 111, 133, 148, 211, 315, 882
MroNI GCCGGC 1 cut(s) 23
MseI TTAA 1 cut(s) 723
MslI CAYNNNNRTG 3 cut(s) 260, 266, 785
MspA1I CMGCKG 1 cut(s) 761
MspCI CTTAAG 1 cut(s) 722
MspI CCGG 4 cut(s) 24, 179, 296, 542
MspR9I CCNGG 4 cut(s) 118, 179, 542, 776
MvaI CCWGG 2 cut(s) 118, 776
MvnI CGCG 3 cut(s) 62, 197, 225
MwoI GCNNNNNNNGC 5 cut(s) 16, 248, 455, 479, 632
NaeI GCCGGC 1 cut(s) 25
NciI CCSGG 2 cut(s) 179, 542
NdeII GATC 6 cut(s) 79, 234, 400, 513, 649, 673
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 2 cut(s) 265, 418
NlaIV GGNNCC 4 cut(s) 177, 183, 439, 595
NruI TCGCGA 1 cut(s) 197
NspI RCATGY 1 cut(s) 418
PciI ACATGT 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 540
PkrI GCNGC 8 cut(s) 150, 189, 241, 253, 303, 457, 760, 864
Ple19I CGATCG 1 cut(s) 237
PleI GAGTC 3 cut(s) 169, 193, 280
PpsI GAGTC 3 cut(s) 169, 193, 280
PpuMI RGGWCCY 1 cut(s) 437
PscI ACATGT 1 cut(s) 414
PshAI GACNNNNGTC 1 cut(s) 125
PsiI TTATAA 1 cut(s) 696
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 116, 774
PspGI CCWGG 2 cut(s) 116, 774
PspN4I GGNNCC 4 cut(s) 177, 183, 439, 595
PspPI GGNCC 4 cut(s) 120, 175, 437, 593
PspPPI RGGWCCY 1 cut(s) 437
PsuI RGATCY 2 cut(s) 400, 513
PvuI CGATCG 1 cut(s) 237
PvuII CAGCTG 1 cut(s) 761
RruI TCGCGA 1 cut(s) 197
RsaI GTAC 1 cut(s) 210
RsaNI GTAC 1 cut(s) 209
RseI CAYNNNNRTG 3 cut(s) 260, 266, 785
SaqAI TTAA 1 cut(s) 723
SatI GCNGC 8 cut(s) 149, 188, 240, 252, 302, 456, 759, 863
Sau3AI GATC 6 cut(s) 79, 234, 400, 513, 649, 673
Sau96I GGNCC 4 cut(s) 120, 175, 437, 593
SchI GAGTC 3 cut(s) 170, 193, 281
ScrFI CCNGG 4 cut(s) 118, 179, 542, 776
SfaNI GCATC 1 cut(s) 19
SinI GGWCC 2 cut(s) 120, 437
SmiMI CAYNNNNRTG 3 cut(s) 260, 266, 785
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse9I AATT 2 cut(s) 468, 526
StyD4I CCNGG 4 cut(s) 116, 177, 540, 774
TaaI ACNGT 2 cut(s) 331, 538
TaiI ACGT 2 cut(s) 93, 288
TaqI TCGA 1 cut(s) 78
TasI AATT 2 cut(s) 468, 526
TatI WGTACW 1 cut(s) 208
TauI GCSGC 4 cut(s) 151, 190, 242, 254
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TscAI CASTG 3 cut(s) 345, 649, 735
TseI GCWGC 4 cut(s) 301, 455, 758, 862
TspDTI ATGAA 1 cut(s) 648
TspGWI ACGGA 2 cut(s) 115, 307
TspRI CASTG 3 cut(s) 345, 649, 735
Vha464I CTTAAG 1 cut(s) 722
VpaK11BI GGWCC 2 cut(s) 120, 437
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 418
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.