Rroxscaffold_3G00223910

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012089
Physical Location & Seq
Forward (+)
7038099 .. 7042910
4812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00223910.1

Sequence Viewer

Length: 879 bp
ATGAGTGAGCAGCATCAGGTAGCCGGCGACACAAAGCCTCCTACTCACTCCCAAGACTACGCTCCTTACCCTAAGCTCGATCCAAACGACGTCGCTCCGCCTCCCCACTCCGAGACCTGGACCTCCGTCTCCATCGCCTCAGAAAAGTCCGAATCGCCGTCGGTCCCGTCAGCGCCGTCCGTCGCGAATCCCGAAGTACACCCCGAGGCTCGGGCACCGCCGATCCCCGCAGACAACGCCGCCACCACCATGCCGCTGGAGTCCAACCCTTACGTCACCAATCCGGCGCCGCCTCCTTCTTCGTCCGTCAAGAATACGGTGGATTCAGTGAAAGATGTTCTTGGAAAATGGGGAAAGAAGGCTGTGGAGACTACCAAGAAGGGTCAGGATCTTGCGGGCAACATGTGGCAACACTTGAAAACAGGACCCAGTTTTGCTGATGCTGCTGTAGGAAGAATTGCTCAGAGCGCAAAAGTGATTGCTGAAGGTGGTTATGAGAAGATCTTTCAGAAAAATTTTGAGACAGTCCCTGACGAGCAACTTCTGAAGACATTTGCTTGCTATCTGTCCACATCTGCTGGCCCCGTAATGGGAGTTATATATTTGTCAACAGCAAAGCTTGCATTTTGCAGTGATGACCCCCTTTCATACAAGACTGGTGATCAGACTGCATGGAGCTATTATAAGGTGGTTATTCCATTACATCAGCTAAAGGCAGTCAACCCATCAACGAGCAAAGTCAAAGCAGCTGAAAAATACATCCAGGTCATCTCTGTTGACAACCATGAGTTCTGGTTTATGAGCTTTGTAAACTATGATAGTGCTGTGAAACACCTTCAAGAAGCAGTGCAGCCCCACCCCCAACCCCACAGCTTGTAA

Protein Analysis

292

Amino Acids

31.68

Weight (kDa)

6.05

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 168 - 284 4.6e-20 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28200 AT1G28200
fragaria_vesca FvH4_5g35910
malus_domestica MD08G1192100.v1.1 MD15G1380800.v1.1
prunus_persica Prupe.1G527000_v2.0.a1
pyrus_communis pycom08g16590 pycom15g34130
rosa_chinensis RchiOBHm_Chr7g0237641
rosa_laevigata RLG00000000979
rosa_multiflora Rmu_sc0007826.1_g000010
rosa_roxburghii Rroxscaffold_3G00223910
rosa_rugosa Rorug07G0305800 Rorug07G0305900
rosa_samantha Rh7AG462600 Rh7BG432600 Rh7CG479400 Rh7DG448400
rosa_wichuraiana Rw7G038270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 2 cut(s) 214, 286
AccII CGCG 1 cut(s) 185
AciI CCGC 7 cut(s) 98, 218, 228, 240, 254, 290, 395
AclWI GGATC 3 cut(s) 74, 217, 396
AcsI RAATTY 1 cut(s) 514
AcuI CTGAAG 2 cut(s) 504, 566
AcyI GRCGYC 2 cut(s) 90, 287
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 4 cut(s) 117, 210, 589, 590
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 2 cut(s) 418, 839
AjnI CCWGG 2 cut(s) 116, 762
AluBI AGCT 7 cut(s) 76, 619, 678, 709, 749, 804, 873
AluI AGCT 7 cut(s) 76, 619, 678, 709, 749, 804, 873
Alw26I GTCTC 4 cut(s) 107, 133, 362, 515
AlwI GGATC 3 cut(s) 74, 217, 396
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 2 cut(s) 203, 210
AoxI GGCC 1 cut(s) 580
ApeKI GCWGC 4 cut(s) 10, 443, 746, 850
ApoI RAATTY 1 cut(s) 514
AspLEI GCGC 3 cut(s) 175, 289, 470
AspS9I GGNCC 4 cut(s) 120, 163, 425, 581
AsuHPI GGTGA 2 cut(s) 268, 671
AvaI CYCGRG 2 cut(s) 203, 210
AvaII GGWCC 3 cut(s) 120, 163, 425
BaeGI GKGCMC 1 cut(s) 217
BanI GGYRCC 2 cut(s) 214, 286
BbsI GAAGAC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 22, 430, 758, 862
BccI CCATC 2 cut(s) 140, 733
BceAI ACGGC 2 cut(s) 142, 160
BciT130I CCWGG 2 cut(s) 118, 764
BclI TGATCA 1 cut(s) 661
BcoDI GTCTC 4 cut(s) 107, 133, 362, 515
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 2 cut(s) 176, 290
BglII AGATCT 1 cut(s) 501
BisI GCNGC 7 cut(s) 11, 240, 254, 290, 444, 747, 851
BlsI GCNGC 7 cut(s) 12, 241, 255, 291, 445, 748, 852
Bme1390I CCNGG 2 cut(s) 118, 764
Bme18I GGWCC 3 cut(s) 120, 163, 425
BmeT110I CYCGRG 2 cut(s) 203, 210
BmgT120I GGNCC 4 cut(s) 120, 163, 425, 581
BmiI GGNNCC 5 cut(s) 165, 216, 288, 427, 583
BmrFI CCNGG 2 cut(s) 118, 764
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 2 cut(s) 22, 430
BmuI ACTGGG 1 cut(s) 423
BoxI GACNNNNGTC 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 554
BpmI CTGGAG 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 72
BsaHI GRCGYC 2 cut(s) 90, 287
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 204
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 4 cut(s) 117, 210, 589, 590
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 2 cut(s) 429, 661
BseBI CCWGG 2 cut(s) 118, 764
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 1 cut(s) 759
BseLI CCNNNNNNNGG 4 cut(s) 117, 210, 589, 590
BseMII CTCAG 2 cut(s) 153, 476
BseNI ACTGG 2 cut(s) 429, 661
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 4 cut(s) 22, 430, 758, 862
BsgI GTGCAG 1 cut(s) 869
Bsh1236I CGCG 1 cut(s) 185
BshFI GGCC 1 cut(s) 582
BshNI GGYRCC 2 cut(s) 214, 286
BsiHKCI CYCGRG 2 cut(s) 203, 210
BsiSI CCGG 2 cut(s) 24, 284
BslFI GGGAC 2 cut(s) 149, 512
BslI CCNNNNNNNGG 4 cut(s) 117, 210, 589, 590
BsmAI GTCTC 4 cut(s) 107, 133, 362, 515
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 149, 512
BsnI GGCC 1 cut(s) 582
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 2 cut(s) 203, 210
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 5 cut(s) 79, 222, 388, 501, 661
Bsp68I TCGCGA 1 cut(s) 185
BspACI CCGC 7 cut(s) 98, 218, 228, 240, 254, 290, 395
BspANI GGCC 1 cut(s) 582
BspCNI CTCAG 2 cut(s) 152, 475
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 5 cut(s) 165, 216, 288, 427, 583
BspPI GGATC 3 cut(s) 74, 217, 396
BspT107I GGYRCC 2 cut(s) 214, 286
BspTNI GGTCTC 1 cut(s) 107
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 2 cut(s) 429, 661
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 5 cut(s) 79, 222, 388, 501, 661
BssNI GRCGYC 2 cut(s) 90, 287
Bst2UI CCWGG 2 cut(s) 118, 764
Bst4CI ACNGT 2 cut(s) 319, 526
BstACI GRCGYC 2 cut(s) 90, 287
BstAPI GCANNNNNTGC 1 cut(s) 620
BstC8I GCNNGC 5 cut(s) 25, 397, 559, 580, 621
BstDEI CTNAG 3 cut(s) 72, 139, 462
BstF5I GGATG 1 cut(s) 759
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 2 cut(s) 176, 290
BstHHI GCGC 3 cut(s) 175, 289, 470
BstKTI GATC 5 cut(s) 82, 225, 391, 504, 664
BstMAI GTCTC 4 cut(s) 107, 133, 362, 515
BstMBI GATC 5 cut(s) 79, 222, 388, 501, 661
BstMWI GCNNNNNNNGC 4 cut(s) 236, 443, 467, 620
BstNI CCWGG 2 cut(s) 118, 764
BstNSI RCATGY 1 cut(s) 406
BstPAI GACNNNNGTC 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 116, 762
BstSFI CTRYAG 1 cut(s) 447
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 4 cut(s) 22, 430, 758, 862
BstV2I GAAGAC 1 cut(s) 554
BstX2I RGATCY 2 cut(s) 388, 501
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 2 cut(s) 388, 501
BsuRI GGCC 1 cut(s) 582
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 759
BtsI GCAGTG 2 cut(s) 637, 852
BtsIMutI CAGTG 3 cut(s) 333, 637, 852
BtuMI TCGCGA 1 cut(s) 185
Cac8I GCNNGC 5 cut(s) 25, 397, 559, 580, 621
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 3 cut(s) 175, 289, 470
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 4 cut(s) 120, 163, 425, 581
Csp6I GTAC 1 cut(s) 197
CviAII CATG 4 cut(s) 250, 403, 672, 785
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 3 cut(s) 72, 139, 462
DinI GGCGCC 1 cut(s) 288
DpnI GATC 5 cut(s) 81, 224, 390, 503, 663
DpnII GATC 5 cut(s) 79, 222, 388, 501, 661
EciI GGCGGA 1 cut(s) 87
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 3 cut(s) 120, 163, 425
Eco57I CTGAAG 2 cut(s) 504, 566
Eco88I CYCGRG 2 cut(s) 203, 210
EcoO109I RGGNCCY 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 116, 762
EgeI GGCGCC 1 cut(s) 288
EheI GGCGCC 1 cut(s) 288
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 4 cut(s) 253, 406, 675, 788
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FaqI GGGAC 2 cut(s) 149, 512
FatI CATG 4 cut(s) 249, 402, 671, 784
FauI CCCGC 2 cut(s) 235, 388
FbaI TGATCA 1 cut(s) 661
Fnu4HI GCNGC 7 cut(s) 11, 240, 254, 290, 444, 747, 851
FokI GGATG 1 cut(s) 746
Fsp4HI GCNGC 7 cut(s) 11, 240, 254, 290, 444, 747, 851
GlaI GCGC 3 cut(s) 174, 288, 469
GluI GCNGC 7 cut(s) 11, 240, 254, 290, 444, 747, 851
GsuI CTGGAG 1 cut(s) 278
HaeII RGCGCY 2 cut(s) 176, 290
HaeIII GGCC 1 cut(s) 582
HapII CCGG 2 cut(s) 24, 284
HhaI GCGC 3 cut(s) 175, 289, 470
Hin1I GRCGYC 2 cut(s) 90, 287
Hin1II CATG 4 cut(s) 253, 406, 675, 788
Hin6I GCGC 3 cut(s) 173, 287, 468
HinP1I GCGC 3 cut(s) 173, 287, 468
HincII GTYRAC 3 cut(s) 609, 721, 778
HindII GTYRAC 3 cut(s) 609, 721, 778
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 4 cut(s) 152, 187, 260, 323
HpaII CCGG 2 cut(s) 24, 284
HphI GGTGA 2 cut(s) 268, 671
Hpy166II GTNNAC 6 cut(s) 199, 570, 609, 721, 778, 811
Hpy188I TCNGA 7 cut(s) 112, 142, 151, 465, 510, 546, 666
Hpy188III TCNNGA 5 cut(s) 184, 191, 310, 386, 839
Hpy8I GTNNAC 6 cut(s) 199, 570, 609, 721, 778, 811
Hpy99I CGWCG 4 cut(s) 92, 95, 163, 185
HpyAV CCTTC 5 cut(s) 306, 352, 373, 479, 845
HpyCH4III ACNGT 2 cut(s) 319, 526
HpyCH4IV ACGT 2 cut(s) 90, 273
HpyCH4V TGCA 4 cut(s) 623, 630, 671, 850
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 443, 467, 620
HpyF3I CTNAG 3 cut(s) 72, 139, 462
HpySE526I ACGT 2 cut(s) 90, 273
Hsp92I GRCGYC 2 cut(s) 90, 287
Hsp92II CATG 4 cut(s) 253, 406, 675, 788
HspAI GCGC 3 cut(s) 173, 287, 468
KasI GGCGCC 1 cut(s) 286
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 5 cut(s) 79, 222, 388, 501, 661
LmnI GCTCC 3 cut(s) 67, 100, 675
Lsp1109I GCAGC 4 cut(s) 22, 430, 758, 862
LweI GCATC 2 cut(s) 22, 430
MaeII ACGT 2 cut(s) 90, 273
MaeIII GTNAC 1 cut(s) 274
MalI GATC 5 cut(s) 81, 224, 390, 503, 663
MboI GATC 5 cut(s) 79, 222, 388, 501, 661
MboII GAAGA 4 cut(s) 291, 465, 511, 559
MflI RGATCY 2 cut(s) 388, 501
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 2 cut(s) 456, 514
Mly113I GGCGCC 1 cut(s) 287
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 6 cut(s) 48, 111, 133, 148, 199, 303
MroNI GCCGGC 1 cut(s) 23
MslI CAYNNNNRTG 1 cut(s) 248
MspA1I CMGCKG 2 cut(s) 256, 749
MspI CCGG 2 cut(s) 24, 284
MspR9I CCNGG 2 cut(s) 118, 764
MvaI CCWGG 2 cut(s) 118, 764
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 4 cut(s) 236, 443, 467, 620
NaeI GCCGGC 1 cut(s) 25
NarI GGCGCC 1 cut(s) 287
NdeII GATC 5 cut(s) 79, 222, 388, 501, 661
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 4 cut(s) 253, 406, 675, 788
NlaIV GGNNCC 5 cut(s) 165, 216, 288, 427, 583
NmuCI GTSAC 1 cut(s) 274
NruI TCGCGA 1 cut(s) 185
NspI RCATGY 1 cut(s) 406
PciI ACATGT 1 cut(s) 402
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 189
PdiI GCCGGC 1 cut(s) 25
PfeI GAWTC 3 cut(s) 152, 187, 323
PkrI GCNGC 7 cut(s) 12, 241, 255, 291, 445, 748, 852
PleI GAGTC 1 cut(s) 268
PluTI GGCGCC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 425
PscI ACATGT 1 cut(s) 402
PshAI GACNNNNGTC 1 cut(s) 125
PsiI TTATAA 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 2 cut(s) 116, 762
PspGI CCWGG 2 cut(s) 116, 762
PspN4I GGNNCC 5 cut(s) 165, 216, 288, 427, 583
PspPI GGNCC 4 cut(s) 120, 163, 425, 581
PspPPI RGGWCCY 1 cut(s) 425
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 2 cut(s) 388, 501
PvuII CAGCTG 1 cut(s) 749
RruI TCGCGA 1 cut(s) 185
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 248
SatI GCNGC 7 cut(s) 11, 240, 254, 290, 444, 747, 851
Sau3AI GATC 5 cut(s) 79, 222, 388, 501, 661
Sau96I GGNCC 4 cut(s) 120, 163, 425, 581
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 2 cut(s) 118, 764
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 2 cut(s) 22, 430
SfcI CTRYAG 1 cut(s) 447
SfoI GGCGCC 1 cut(s) 288
SinI GGWCC 3 cut(s) 120, 163, 425
SmiMI CAYNNNNRTG 1 cut(s) 248
Sse9I AATT 2 cut(s) 456, 514
SsiI CCGC 7 cut(s) 98, 218, 228, 240, 254, 290, 395
SspDI GGCGCC 1 cut(s) 286
StyD4I CCNGG 2 cut(s) 116, 762
TaaI ACNGT 2 cut(s) 319, 526
TaiI ACGT 2 cut(s) 93, 276
TaqI TCGA 1 cut(s) 78
TaqII GACCGA 1 cut(s) 151
TasI AATT 2 cut(s) 456, 514
TatI WGTACW 1 cut(s) 196
TauI GCSGC 3 cut(s) 242, 256, 292
TfiI GAWTC 3 cut(s) 152, 187, 323
TscAI CASTG 3 cut(s) 333, 637, 852
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 4 cut(s) 10, 443, 746, 850
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 1 cut(s) 636
TspGWI ACGGA 3 cut(s) 115, 169, 295
TspRI CASTG 3 cut(s) 333, 637, 852
VpaK11BI GGWCC 3 cut(s) 120, 163, 425
XapI RAATTY 1 cut(s) 514
XceI RCATGY 1 cut(s) 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.