Rh7AG462600

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012089
Physical Location & Seq
Reverse (-)
63705551 .. 63710672
5122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG462600.1

Sequence Viewer

Length: 696 bp
ATGAGTGAGCAGCATCAGGTAGCCGGCGACACAAAGCCTCCTACTCACTCCCAAGACTACGCTCCTTACCCTAAGCTCGATCCAAACGACGTCGCTCCGCCTCCCCACTCCGAAACCTGGACCTCCGTCTCCATCGCCTCAGAAAAGTCCGAATCGCCGTCAGTCCCGTCAGCGCCGTCGGTCGCGAATCCCGAAGTACACCCCGAGGCTCGGGCACCGCCGATCCCCGAAGACAACGCCGCCGCCACCACCATGCCGCTGGAGTCCAACCCTTACGTCACCAATCCGGCTCCGCCTCCTTCTTCGTCCGTCAAGAATAAGGTGGATTCAGTGAAAGATGTTCTTGGAAAATGGGGAAAGAAGGCTGTGGAGACTACCAAGAAGGGTCAGGATCTTGCGGGCAACATGTGGCAACACTTGAAAACAGGACCCAGTTTTGCTGATGCTGCTGTAGGAAGAATTGCTCAGAGTGCAAAAGTGATTGCTGAAGGTGGTTATGAGAAGATCTTTCAGAAAAATTTTGAGACAGTCCCTGACGAGCAACTTCTGAAGACATTTGCTTGCTATCTGTCCACATCTGCTGGCCCCGTAATGGGAGTTATATATTTGTCAACAGCAAAGCTTGCATTTTGCAGTGATGACCCCCTTTCATACAAGACTGGTGATCAGACTGCATGGAGCTATTATAAGGTATAG

Protein Analysis

231

Amino Acids

24.77

Weight (kDa)

5.55

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 169 - 215 2.3e-08 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28200 AT1G28200
fragaria_vesca FvH4_5g35910
malus_domestica MD08G1192100.v1.1 MD15G1380800.v1.1
prunus_persica Prupe.1G527000_v2.0.a1
pyrus_communis pycom08g16590 pycom15g34130
rosa_chinensis RchiOBHm_Chr7g0237641
rosa_laevigata RLG00000000979
rosa_multiflora Rmu_sc0007826.1_g000010
rosa_roxburghii Rroxscaffold_3G00223910
rosa_rugosa Rorug07G0305800 Rorug07G0305900
rosa_samantha Rh7AG462600 Rh7BG432600 Rh7CG479400 Rh7DG448400
rosa_wichuraiana Rw7G038270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 214
AccII CGCG 1 cut(s) 185
AciI CCGC 7 cut(s) 98, 218, 240, 243, 257, 293, 398
AclWI GGATC 3 cut(s) 74, 217, 399
AcsI RAATTY 1 cut(s) 517
AcuI CTGAAG 2 cut(s) 507, 569
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 4 cut(s) 117, 210, 592, 593
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 1 cut(s) 421
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 3 cut(s) 76, 622, 681
AluI AGCT 3 cut(s) 76, 622, 681
Alw26I GTCTC 3 cut(s) 133, 365, 518
AlwI GGATC 3 cut(s) 74, 217, 399
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 2 cut(s) 203, 210
AoxI GGCC 1 cut(s) 583
ApeKI GCWGC 2 cut(s) 10, 446
ApoI RAATTY 1 cut(s) 517
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 3 cut(s) 120, 428, 584
AsuHPI GGTGA 2 cut(s) 271, 674
AvaI CYCGRG 2 cut(s) 203, 210
AvaII GGWCC 2 cut(s) 120, 428
BaeGI GKGCMC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 214
BbsI GAAGAC 2 cut(s) 237, 557
BbvI GCAGC 2 cut(s) 22, 433
BccI CCATC 1 cut(s) 140
BceAI ACGGC 2 cut(s) 142, 160
BciT130I CCWGG 1 cut(s) 118
BclI TGATCA 1 cut(s) 664
BcoDI GTCTC 3 cut(s) 133, 365, 518
BfmI CTRYAG 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 176
BglII AGATCT 1 cut(s) 504
BisI GCNGC 5 cut(s) 11, 240, 243, 257, 447
BlsI GCNGC 5 cut(s) 12, 241, 244, 258, 448
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 2 cut(s) 120, 428
BmeT110I CYCGRG 2 cut(s) 203, 210
BmgT120I GGNCC 3 cut(s) 120, 428, 584
BmiI GGNNCC 4 cut(s) 216, 291, 430, 586
BmrFI CCNGG 1 cut(s) 118
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 2 cut(s) 22, 433
BmuI ACTGGG 1 cut(s) 426
BoxI GACNNNNGTC 1 cut(s) 125
BpiI GAAGAC 2 cut(s) 237, 557
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 204
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 4 cut(s) 117, 210, 592, 593
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 2 cut(s) 432, 664
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 204
BseLI CCNNNNNNNGG 4 cut(s) 117, 210, 592, 593
BseMII CTCAG 2 cut(s) 153, 479
BseNI ACTGG 2 cut(s) 432, 664
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 22, 433
Bsh1236I CGCG 1 cut(s) 185
Bsh1285I CGRYCG 1 cut(s) 183
BshFI GGCC 1 cut(s) 585
BshNI GGYRCC 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 183
BsiHKCI CYCGRG 2 cut(s) 203, 210
BsiSI CCGG 2 cut(s) 24, 287
BslFI GGGAC 2 cut(s) 149, 515
BslI CCNNNNNNNGG 4 cut(s) 117, 210, 592, 593
BsmAI GTCTC 3 cut(s) 133, 365, 518
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 149, 515
BsnI GGCC 1 cut(s) 585
BsoBI CYCGRG 2 cut(s) 203, 210
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 5 cut(s) 79, 222, 391, 504, 664
Bsp68I TCGCGA 1 cut(s) 185
BspACI CCGC 7 cut(s) 98, 218, 240, 243, 257, 293, 398
BspANI GGCC 1 cut(s) 585
BspCNI CTCAG 2 cut(s) 152, 478
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 4 cut(s) 216, 291, 430, 586
BspPI GGATC 3 cut(s) 74, 217, 399
BspT107I GGYRCC 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 2 cut(s) 432, 664
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 5 cut(s) 79, 222, 391, 504, 664
BssNI GRCGYC 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 529
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 623
BstC8I GCNNGC 5 cut(s) 25, 400, 562, 583, 624
BstDEI CTNAG 3 cut(s) 72, 139, 465
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 5 cut(s) 82, 225, 394, 507, 667
BstMAI GTCTC 3 cut(s) 133, 365, 518
BstMBI GATC 5 cut(s) 79, 222, 391, 504, 664
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 3 cut(s) 446, 470, 623
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 1 cut(s) 409
BstPAI GACNNNNGTC 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 450
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 2 cut(s) 22, 433
BstV2I GAAGAC 2 cut(s) 237, 557
BstX2I RGATCY 2 cut(s) 391, 504
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 2 cut(s) 391, 504
BsuRI GGCC 1 cut(s) 585
BtgZI GCGATG 1 cut(s) 118
BtsI GCAGTG 1 cut(s) 640
BtsIMutI CAGTG 2 cut(s) 336, 640
BtuMI TCGCGA 1 cut(s) 185
Cac8I GCNNGC 5 cut(s) 25, 400, 562, 583, 624
CaiI CAGNNNCTG 1 cut(s) 533
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 3 cut(s) 120, 428, 584
Csp6I GTAC 1 cut(s) 197
CviAII CATG 3 cut(s) 253, 406, 675
CviJI RGCY 9 cut(s) 23, 37, 76, 209, 290, 365, 585, 622, 681
CviKI_1 RGCY 9 cut(s) 23, 37, 76, 209, 290, 365, 585, 622, 681
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 3 cut(s) 72, 139, 465
DpnI GATC 5 cut(s) 81, 224, 393, 506, 666
DpnII GATC 5 cut(s) 79, 222, 391, 504, 664
EciI GGCGGA 2 cut(s) 87, 282
Eco47I GGWCC 2 cut(s) 120, 428
Eco57I CTGAAG 2 cut(s) 507, 569
Eco88I CYCGRG 2 cut(s) 203, 210
EcoO109I RGGNCCY 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 116
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 3 cut(s) 256, 409, 678
FaiI YATR 9 cut(s) 254, 407, 498, 602, 604, 652, 676, 687, 694
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 2 cut(s) 149, 515
FatI CATG 3 cut(s) 252, 405, 674
FauI CCCGC 1 cut(s) 391
FbaI TGATCA 1 cut(s) 664
Fnu4HI GCNGC 5 cut(s) 11, 240, 243, 257, 447
Fsp4HI GCNGC 5 cut(s) 11, 240, 243, 257, 447
GlaI GCGC 1 cut(s) 174
GluI GCNGC 5 cut(s) 11, 240, 243, 257, 447
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 585
HapII CCGG 2 cut(s) 24, 287
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 3 cut(s) 256, 409, 678
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 4 cut(s) 152, 187, 263, 326
HpaII CCGG 2 cut(s) 24, 287
HphI GGTGA 2 cut(s) 271, 674
Hpy166II GTNNAC 3 cut(s) 199, 573, 612
Hpy188I TCNGA 7 cut(s) 112, 142, 151, 468, 513, 549, 669
Hpy188III TCNNGA 4 cut(s) 184, 191, 313, 389
Hpy8I GTNNAC 3 cut(s) 199, 573, 612
Hpy99I CGWCG 3 cut(s) 92, 95, 181
HpyAV CCTTC 4 cut(s) 309, 355, 376, 482
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 2 cut(s) 90, 276
HpyCH4V TGCA 4 cut(s) 473, 626, 633, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 470, 623
HpyF3I CTNAG 3 cut(s) 72, 139, 465
HpySE526I ACGT 2 cut(s) 90, 276
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 3 cut(s) 256, 409, 678
HspAI GCGC 1 cut(s) 173
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 5 cut(s) 79, 222, 391, 504, 664
LmnI GCTCC 4 cut(s) 67, 100, 295, 678
Lsp1109I GCAGC 2 cut(s) 22, 433
LweI GCATC 2 cut(s) 22, 433
MaeII ACGT 2 cut(s) 90, 276
MaeIII GTNAC 1 cut(s) 277
MalI GATC 5 cut(s) 81, 224, 393, 506, 666
MboI GATC 5 cut(s) 79, 222, 391, 504, 664
MboII GAAGA 5 cut(s) 242, 294, 468, 514, 562
MflI RGATCY 2 cut(s) 391, 504
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 2 cut(s) 459, 517
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 6 cut(s) 48, 111, 133, 148, 199, 306
MroNI GCCGGC 1 cut(s) 23
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 2 cut(s) 24, 287
MspR9I CCNGG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 3 cut(s) 446, 470, 623
NaeI GCCGGC 1 cut(s) 25
NdeII GATC 5 cut(s) 79, 222, 391, 504, 664
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 3 cut(s) 256, 409, 678
NlaIV GGNNCC 4 cut(s) 216, 291, 430, 586
NmuCI GTSAC 1 cut(s) 277
NruI TCGCGA 1 cut(s) 185
NspI RCATGY 1 cut(s) 409
PciI ACATGT 1 cut(s) 405
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 189
PdiI GCCGGC 1 cut(s) 25
PfeI GAWTC 3 cut(s) 152, 187, 326
PkrI GCNGC 5 cut(s) 12, 241, 244, 258, 448
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 428
PscI ACATGT 1 cut(s) 405
PshAI GACNNNNGTC 1 cut(s) 125
PsiI TTATAA 1 cut(s) 687
Psp5II RGGWCCY 1 cut(s) 428
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 4 cut(s) 216, 291, 430, 586
PspPI GGNCC 3 cut(s) 120, 428, 584
PspPPI RGGWCCY 1 cut(s) 428
PstNI CAGNNNCTG 1 cut(s) 533
PsuI RGATCY 2 cut(s) 391, 504
RruI TCGCGA 1 cut(s) 185
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 251
SatI GCNGC 5 cut(s) 11, 240, 243, 257, 447
Sau3AI GATC 5 cut(s) 79, 222, 391, 504, 664
Sau96I GGNCC 3 cut(s) 120, 428, 584
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 2 cut(s) 22, 433
SfcI CTRYAG 1 cut(s) 450
SinI GGWCC 2 cut(s) 120, 428
SmiMI CAYNNNNRTG 1 cut(s) 251
Sse9I AATT 2 cut(s) 459, 517
SsiI CCGC 7 cut(s) 98, 218, 240, 243, 257, 293, 398
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 2 cut(s) 93, 279
TaqI TCGA 1 cut(s) 78
TaqII GACCGA 1 cut(s) 169
TasI AATT 2 cut(s) 459, 517
TatI WGTACW 1 cut(s) 196
TauI GCSGC 3 cut(s) 242, 245, 259
TfiI GAWTC 3 cut(s) 152, 187, 326
TscAI CASTG 2 cut(s) 336, 640
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 2 cut(s) 10, 446
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 1 cut(s) 639
TspGWI ACGGA 2 cut(s) 115, 298
TspRI CASTG 2 cut(s) 336, 640
VpaK11BI GGWCC 2 cut(s) 120, 428
XapI RAATTY 1 cut(s) 517
XceI RCATGY 1 cut(s) 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.