Prupe.1G527000_v2.0.a1

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012089
Physical Location & Seq
Forward (+)
43143833 .. 43147188
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G527000.1

Sequence Viewer

Length: 921 bp
ATGAGCGACCACAAGACCGCTGCAGCAGCTGCAGCACCCGATCACCACCCACCATCTCAGACCGCGAAGCCTCTTCCTCACTCCGAAGACTACGCTCCCTACCCTAAGCTCGACCCCAAGGACGTCGTGCCGCCTCCCCAGCCCGAAACTTGGACCTCTGTCTCTATCGCCTCTGAACCGCCGCCGAAATTGGAATCACCGTCAGCGCCGCCGCCTCCAGTTGCCGATCCTCCGGTTCACGTCGAGGCTCGAGCGCCGATTGCCGGCGAAGCTGCCACCTCCATGCCGACGGAGTCCAATCCCTACGTCGCCACTCCTGCTCCGGCTCCCTCCTCCTCCGTCAAGAATAAGATGGATTCAGTGAAAGATGTTCTTGGAAAATGGGGAAAGAAGGCTGCGGAGGCCACCAAGAAGGCCGAGGATCTTGCTGGCAACATGTGGCAGCACTTGAAAACGGGACCTAGTTTTGCTGATGCTGCTGTGGCAAGAATTGCGCAGAGCACTAAGGTACTTGCAGAAGGTGGTTATGAGAAGATCTTTCGACAAAATTTTGAGACTGTCCCAGAGGAGCAACTTCAGAAGACATATGCTTGCTACCTATCCACATCTGCTGGCCCAGTAATGGGAATTTTATATTTGTCAACGGCAAAGCTTGCATTTTGCAGTGACGATCCCCTTTCATACAAAAATGGCGACCAGACTGAATGGAGCTATTATAAGGTCGTTATTCCATTGCACCAGCTGAAGGCAGTCAATCCATCAACAAGCAAAGTCAAATCAGCTGAAAAATACATCCAGGTGGTCTCTGTTGACAACCATGAATTCTGGTTTATGAGCTTTGTACACTATGACAGTGCTGTGAAACATCTTCAAGAAATATTGCACCCCACCCAGCCCCAGCCCCAGCCCCAGAGTTTGTGA

Protein Analysis

307

Amino Acids

33.24

Weight (kDa)

6.12

Isoelectric Point (pI)

56.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28200 AT1G28200
fragaria_vesca FvH4_5g35910
malus_domestica MD08G1192100.v1.1 MD15G1380800.v1.1
prunus_persica Prupe.1G527000_v2.0.a1
pyrus_communis pycom08g16590 pycom15g34130
rosa_chinensis RchiOBHm_Chr7g0237641
rosa_laevigata RLG00000000979
rosa_multiflora Rmu_sc0007826.1_g000010
rosa_roxburghii Rroxscaffold_3G00223910
rosa_rugosa Rorug07G0305800 Rorug07G0305900
rosa_samantha Rh7AG462600 Rh7BG432600 Rh7CG479400 Rh7DG448400
rosa_wichuraiana Rw7G038270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
AatII GACGTC 1 cut(s) 126
Acc16I TGCGCA 1 cut(s) 495
AccII CGCG 1 cut(s) 65
AciI CCGC 8 cut(s) 18, 63, 131, 179, 182, 209, 212, 398
AclWI GGATC 3 cut(s) 221, 429, 665
AcsI RAATTY 3 cut(s) 547, 627, 821
AcuI CTGAAG 2 cut(s) 560, 764
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 2 cut(s) 510, 843
AfiI CCNNNNNNNGG 5 cut(s) 150, 263, 622, 623, 745
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 2 cut(s) 451, 872
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 1 cut(s) 795
AluBI AGCT 8 cut(s) 29, 109, 272, 652, 711, 742, 782, 837
AluI AGCT 8 cut(s) 29, 109, 272, 652, 711, 742, 782, 837
Alw21I GWGCWC 1 cut(s) 503
Alw26I GTCTC 3 cut(s) 166, 548, 808
AlwI GGATC 3 cut(s) 221, 429, 665
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 249
AoxI GGCC 3 cut(s) 402, 414, 613
ApeKI GCWGC 9 cut(s) 20, 23, 26, 29, 32, 272, 395, 442, 476
ApoI RAATTY 3 cut(s) 547, 627, 821
AspLEI GCGC 3 cut(s) 208, 256, 496
AspS9I GGNCC 3 cut(s) 153, 458, 614
AsuHPI GGTGA 2 cut(s) 35, 189
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 2 cut(s) 153, 458
BbsI GAAGAC 2 cut(s) 93, 587
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 9 cut(s) 7, 16, 35, 38, 44, 259, 382, 454, 463
BccI CCATC 3 cut(s) 61, 346, 766
BceAI ACGGC 1 cut(s) 660
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 1 cut(s) 797
BcoDI GTCTC 3 cut(s) 166, 548, 808
BfaI CTAG 1 cut(s) 462
BfmI CTRYAG 2 cut(s) 21, 30
BfoI RGCGCY 2 cut(s) 209, 257
BglII AGATCT 1 cut(s) 534
Bme1390I CCNGG 1 cut(s) 797
Bme18I GGWCC 2 cut(s) 153, 458
BmeT110I CYCGRG 1 cut(s) 249
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 153, 458, 614
BmiI GGNNCC 2 cut(s) 327, 459
BmrFI CCNGG 1 cut(s) 797
BmrI ACTGGG 1 cut(s) 611
BmsI GCATC 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 611
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 2 cut(s) 93, 587
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 808
BsaJI CCNNGG 2 cut(s) 117, 417
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 5 cut(s) 150, 263, 622, 623, 745
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 218, 617
Bse3DI GCAATG 1 cut(s) 731
BseBI CCWGG 1 cut(s) 797
BseDI CCNNGG 2 cut(s) 117, 417
BseGI GGATG 1 cut(s) 792
BseLI CCNNNNNNNGG 5 cut(s) 150, 263, 622, 623, 745
BseMI GCAATG 1 cut(s) 731
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 2 cut(s) 218, 617
BseRI GAGGAG 3 cut(s) 322, 325, 581
BseXI GCAGC 9 cut(s) 7, 16, 35, 38, 44, 259, 382, 454, 463
BseYI CCCAGC 4 cut(s) 138, 891, 897, 903
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 3 cut(s) 404, 416, 615
BsiHKAI GWGCWC 1 cut(s) 503
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 3 cut(s) 233, 264, 323
BslFI GGGAC 2 cut(s) 471, 545
BslI CCNNNNNNNGG 5 cut(s) 150, 263, 622, 623, 745
BsmAI GTCTC 3 cut(s) 166, 548, 808
BsmFI GGGAC 2 cut(s) 471, 545
BsnI GGCC 3 cut(s) 404, 416, 615
Bso31I GGTCTC 1 cut(s) 808
BsoBI CYCGRG 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 503
Bsp1407I TGTACA 1 cut(s) 841
Bsp143I GATC 5 cut(s) 40, 226, 421, 534, 670
BspACI CCGC 8 cut(s) 18, 63, 131, 179, 182, 209, 212, 398
BspANI GGCC 3 cut(s) 404, 416, 615
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 2 cut(s) 327, 459
BspMAI CTGCAG 2 cut(s) 25, 34
BspPI GGATC 3 cut(s) 221, 429, 665
BspTNI GGTCTC 1 cut(s) 808
BsrDI GCAATG 1 cut(s) 731
BsrFI RCCGGY 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 841
BsrI ACTGG 2 cut(s) 218, 617
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 117, 417
BssMI GATC 5 cut(s) 40, 226, 421, 534, 670
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 1 cut(s) 797
Bst4CI ACNGT 3 cut(s) 201, 559, 854
Bst6I CTCTTC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 3 cut(s) 29, 491, 653
BstAUI TGTACA 1 cut(s) 841
BstC8I GCNNGC 5 cut(s) 265, 430, 592, 613, 654
BstDEI CTNAG 3 cut(s) 57, 105, 504
BstF5I GGATG 1 cut(s) 792
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 2 cut(s) 209, 257
BstHHI GCGC 3 cut(s) 208, 256, 496
BstKTI GATC 5 cut(s) 43, 229, 424, 537, 673
BstMAI GTCTC 3 cut(s) 166, 548, 808
BstMBI GATC 5 cut(s) 40, 226, 421, 534, 670
BstNI CCWGG 1 cut(s) 797
BstNSI RCATGY 1 cut(s) 439
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 795
BstSFI CTRYAG 2 cut(s) 21, 30
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 9 cut(s) 7, 16, 35, 38, 44, 259, 382, 454, 463
BstV2I GAAGAC 2 cut(s) 93, 587
BstX2I RGATCY 2 cut(s) 421, 534
BstYI RGATCY 2 cut(s) 421, 534
BsuRI GGCC 3 cut(s) 404, 416, 615
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 1 cut(s) 792
BtsI GCAGTG 1 cut(s) 670
BtsIMutI CAGTG 3 cut(s) 366, 670, 859
Cac8I GCNNGC 5 cut(s) 265, 430, 592, 613, 654
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 3 cut(s) 208, 256, 496
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 153, 458, 614
Csp6I GTAC 2 cut(s) 509, 842
CviAII CATG 3 cut(s) 283, 436, 818
CviQI GTAC 2 cut(s) 509, 842
DdeI CTNAG 3 cut(s) 57, 105, 504
DpnI GATC 5 cut(s) 42, 228, 423, 536, 672
DpnII GATC 5 cut(s) 40, 226, 421, 534, 670
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 808
Eco47I GGWCC 2 cut(s) 153, 458
Eco57I CTGAAG 2 cut(s) 560, 764
Eco88I CYCGRG 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 821
EcoRII CCWGG 1 cut(s) 795
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 3 cut(s) 286, 439, 821
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 471, 545
FatI CATG 3 cut(s) 282, 435, 817
FauNDI CATATG 1 cut(s) 586
FokI GGATG 1 cut(s) 779
FspBI CTAG 1 cut(s) 462
FspI TGCGCA 1 cut(s) 495
GlaI GCGC 3 cut(s) 207, 255, 495
GsaI CCCAGC 4 cut(s) 142, 895, 901, 907
GsuI CTGGAG 1 cut(s) 201
HaeII RGCGCY 2 cut(s) 209, 257
HaeIII GGCC 3 cut(s) 404, 416, 615
HapII CCGG 3 cut(s) 233, 264, 323
HhaI GCGC 3 cut(s) 208, 256, 496
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 3 cut(s) 286, 439, 821
Hin6I GCGC 3 cut(s) 206, 254, 494
HinP1I GCGC 3 cut(s) 206, 254, 494
HincII GTYRAC 2 cut(s) 642, 811
HindII GTYRAC 2 cut(s) 642, 811
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 3 cut(s) 194, 293, 356
HpaII CCGG 3 cut(s) 233, 264, 323
HphI GGTGA 2 cut(s) 35, 189
Hpy166II GTNNAC 4 cut(s) 238, 642, 811, 844
Hpy188I TCNGA 4 cut(s) 60, 85, 175, 579
Hpy188III TCNNGA 2 cut(s) 343, 872
Hpy8I GTNNAC 4 cut(s) 238, 642, 811, 844
Hpy99I CGWCG 4 cut(s) 128, 245, 292, 311
HpyAV CCTTC 4 cut(s) 385, 406, 512, 739
HpyCH4III ACNGT 3 cut(s) 201, 559, 854
HpyCH4IV ACGT 3 cut(s) 123, 240, 306
HpyCH4V TGCA 7 cut(s) 23, 32, 515, 656, 663, 736, 883
HpyF3I CTNAG 3 cut(s) 57, 105, 504
HpySE526I ACGT 3 cut(s) 123, 240, 306
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 3 cut(s) 286, 439, 821
HspAI GCGC 3 cut(s) 206, 254, 494
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Kzo9I GATC 5 cut(s) 40, 226, 421, 534, 670
LmnI GCTCC 5 cut(s) 100, 325, 331, 568, 708
Lsp1109I GCAGC 9 cut(s) 7, 16, 35, 38, 44, 259, 382, 454, 463
LweI GCATC 1 cut(s) 463
MaeI CTAG 1 cut(s) 462
MaeII ACGT 3 cut(s) 123, 240, 306
MaeIII GTNAC 1 cut(s) 665
MalI GATC 5 cut(s) 42, 228, 423, 536, 672
MboI GATC 5 cut(s) 40, 226, 421, 534, 670
MboII GAAGA 5 cut(s) 65, 98, 544, 592, 860
MflI RGATCY 2 cut(s) 421, 534
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 5 cut(s) 188, 489, 547, 627, 821
MlyI GAGTC 1 cut(s) 302
MroNI GCCGGC 1 cut(s) 263
MslI CAYNNNNRTG 2 cut(s) 281, 797
MspA1I CMGCKG 4 cut(s) 20, 29, 742, 782
MspI CCGG 3 cut(s) 233, 264, 323
MspR9I CCNGG 1 cut(s) 797
MvaI CCWGG 1 cut(s) 797
MvnI CGCG 1 cut(s) 65
NaeI GCCGGC 1 cut(s) 265
NdeI CATATG 1 cut(s) 586
NdeII GATC 5 cut(s) 40, 226, 421, 534, 670
NgoMIV GCCGGC 1 cut(s) 263
NlaIII CATG 3 cut(s) 286, 439, 821
NlaIV GGNNCC 2 cut(s) 327, 459
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 665
NsbI TGCGCA 1 cut(s) 495
NspI RCATGY 1 cut(s) 439
PaeR7I CTCGAG 1 cut(s) 249
PciI ACATGT 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 265
PfeI GAWTC 2 cut(s) 194, 356
PflFI GACNNNGTC 1 cut(s) 292
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
PpuMI RGGWCCY 1 cut(s) 458
PscI ACATGT 1 cut(s) 435
PshAI GACNNNNGTC 1 cut(s) 158
PsiI TTATAA 1 cut(s) 717
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 795
PspFI CCCAGC 4 cut(s) 138, 891, 897, 903
PspGI CCWGG 1 cut(s) 795
PspN4I GGNNCC 2 cut(s) 327, 459
PspPI GGNCC 3 cut(s) 153, 458, 614
PspPPI RGGWCCY 1 cut(s) 458
PspXI VCTCGAGB 1 cut(s) 249
PstI CTGCAG 2 cut(s) 25, 34
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 2 cut(s) 421, 534
PsyI GACNNNGTC 1 cut(s) 292
PvuII CAGCTG 3 cut(s) 29, 742, 782
RsaI GTAC 2 cut(s) 510, 843
RsaNI GTAC 2 cut(s) 509, 842
RseI CAYNNNNRTG 2 cut(s) 281, 797
Sau3AI GATC 5 cut(s) 40, 226, 421, 534, 670
Sau96I GGNCC 3 cut(s) 153, 458, 614
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 1 cut(s) 797
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 2 cut(s) 21, 30
Sfr274I CTCGAG 1 cut(s) 249
SinI GGWCC 2 cut(s) 153, 458
SlaI CTCGAG 1 cut(s) 249
SmiMI CAYNNNNRTG 2 cut(s) 281, 797
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
Sse9I AATT 5 cut(s) 188, 489, 547, 627, 821
SsiI CCGC 8 cut(s) 18, 63, 131, 179, 182, 209, 212, 398
SspI AATATT 1 cut(s) 879
SspMI CTAG 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 795
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 3 cut(s) 201, 559, 854
TaiI ACGT 3 cut(s) 126, 243, 309
TaqI TCGA 4 cut(s) 111, 243, 250, 541
TasI AATT 5 cut(s) 188, 489, 547, 627, 821
TatI WGTACW 1 cut(s) 841
TauI GCSGC 4 cut(s) 133, 184, 211, 214
TfiI GAWTC 2 cut(s) 194, 356
TscAI CASTG 3 cut(s) 366, 670, 859
TseFI GTSAC 1 cut(s) 665
TseI GCWGC 9 cut(s) 20, 23, 26, 29, 32, 272, 395, 442, 476
Tsp45I GTSAC 1 cut(s) 665
TspDTI ATGAA 2 cut(s) 669, 834
TspGWI ACGGA 2 cut(s) 305, 328
TspRI CASTG 3 cut(s) 366, 670, 859
Tth111I GACNNNGTC 1 cut(s) 292
VpaK11BI GGWCC 2 cut(s) 153, 458
XapI RAATTY 3 cut(s) 547, 627, 821
XceI RCATGY 1 cut(s) 439
XhoI CTCGAG 1 cut(s) 249
XspI CTAG 1 cut(s) 462
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.