MD08G1192100.v1.1

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012089
Physical Location & Seq
Forward (+)
24631435 .. 24635337
3903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1192100.v1.1.491

Sequence Viewer

Length: 897 bp
ATGAGCGACCACAAGGTGGCAGCAGCACCGCCTGCACCTGATCACCCGCCACCACCATCTCACTCCGAAGACTACGCTCCGTACCCTAAGGTCGATCTCAACGACGTCGCTCCGCCTCCCCACTCTGAAACTTGGACCTCCGTCTCCATCGCCTCTGAAACGCCTCCGCTCCCGAAGTCGGAGGGCCCGCCGGTGCAGCCGCCTGTTCCGCCGCCGGTGGTTCACGCCGAGGCTCGCGCTCCGATCCCCGGCAACGCCGCCACCTCCATGCCGGCGGAGTCCAATCCCTACGTCGCCACTCCTGCGCCGCCTCCCCCCTCCTCCGTCAAGAAATCGATGGATTCAGTGAAAGATGCTCTCGGAAAATGGGGAAAGAAGGCTGCGGAGGCCACCAAGAAGGCCGAGGATCTTGCTGGCAACATGTGGCAACACTTGAAAACGGGACCCAGTTTTGCTGATGCCGCTGTGACAAGAATTGCTCAGAGCACAAAAGTACTTGCAGAAGGTGGTTATGAGAAGATCTTTCGACACAATTTTGAGACTGTCCCAGAGGAGCAACTTCTAAAGACATTTGCTTGCTACCTATCCACATCTGCTGGCCCAGTAATGGGAATTTTATATTTGTCATCAGCTAAGCTTGCATTTTGCAGTGACGATCCCCTTTCATACACAGTTGGTGACCGGACTGAATGGAGCTATTATAAGGTGATTATTCCATTACATCAGCTGAAGGCAGTCAATCCATCAACGAGCAAAGTGAAAGCAGCTGAAAAGTACATCCAGGTCGTCTCTGTTGACAACCATGAGTTTTGGTTCATGAGCTTTGTGCACTACGATAGTGCTGTGAAACATCTTCAAGAAGCGCTGCAGCCGCATCCTCATCCCCACAGCGTATGA

Protein Analysis

299

Amino Acids

32.26

Weight (kDa)

6.26

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 174 - 290 4.6e-19 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28200 AT1G28200
fragaria_vesca FvH4_5g35910
malus_domestica MD08G1192100.v1.1 MD15G1380800.v1.1
prunus_persica Prupe.1G527000_v2.0.a1
pyrus_communis pycom08g16590 pycom15g34130
rosa_chinensis RchiOBHm_Chr7g0237641
rosa_laevigata RLG00000000979
rosa_multiflora Rmu_sc0007826.1_g000010
rosa_roxburghii Rroxscaffold_3G00223910
rosa_rugosa Rorug07G0305800 Rorug07G0305900
rosa_samantha Rh7AG462600 Rh7BG432600 Rh7CG479400 Rh7DG448400
rosa_wichuraiana Rw7G038270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AatII GACGTC 1 cut(s) 108
AccB7I CCANNNNNTGG 1 cut(s) 16
AccBSI CCGCTC 1 cut(s) 169
AccII CGCG 1 cut(s) 237
AclWI GGATC 3 cut(s) 238, 414, 650
AcsI RAATTY 1 cut(s) 612
AcuI CTGAAG 1 cut(s) 749
AcyI GRCGYC 1 cut(s) 105
AdeI CACNNNGTG 1 cut(s) 16
AfaI GTAC 3 cut(s) 83, 495, 776
AfeI AGCGCT 1 cut(s) 864
AfiI CCNNNNNNNGG 5 cut(s) 16, 178, 248, 607, 608
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 2 cut(s) 436, 857
AjnI CCWGG 1 cut(s) 780
AluBI AGCT 6 cut(s) 632, 637, 696, 727, 767, 822
AluI AGCT 6 cut(s) 632, 637, 696, 727, 767, 822
Alw21I GWGCWC 2 cut(s) 488, 831
Alw26I GTCTC 3 cut(s) 148, 533, 793
Alw44I GTGCAC 1 cut(s) 827
AlwI GGATC 3 cut(s) 238, 414, 650
Aor51HI AGCGCT 1 cut(s) 864
AoxI GGCC 4 cut(s) 184, 387, 399, 598
ApaI GGGCCC 1 cut(s) 188
ApaLI GTGCAC 1 cut(s) 827
ApeKI GCWGC 7 cut(s) 20, 23, 196, 380, 764, 865, 868
ApoI RAATTY 1 cut(s) 612
AspLEI GCGC 3 cut(s) 239, 307, 865
AspS9I GGNCC 5 cut(s) 135, 184, 185, 443, 599
AsuC2I CCSGG 1 cut(s) 249
AsuHPI GGTGA 3 cut(s) 35, 689, 718
AvaII GGWCC 2 cut(s) 135, 443
AxyI CCTNAGG 1 cut(s) 87
BaeGI GKGCMC 2 cut(s) 188, 831
BanII GRGCYC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 488, 831
BbvI GCAGC 7 cut(s) 32, 35, 208, 367, 776, 852, 880
BccI CCATC 4 cut(s) 64, 155, 331, 751
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 1 cut(s) 782
BclI TGATCA 1 cut(s) 40
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 3 cut(s) 148, 533, 793
BfmI CTRYAG 1 cut(s) 866
BfoI RGCGCY 1 cut(s) 866
BglII AGATCT 1 cut(s) 519
BlpI GCTNAGC 1 cut(s) 633
BmcAI AGTACT 1 cut(s) 495
Bme1390I CCNGG 2 cut(s) 249, 782
Bme18I GGWCC 2 cut(s) 135, 443
BmgT120I GGNCC 5 cut(s) 135, 184, 185, 443, 599
BmiI GGNNCC 3 cut(s) 186, 444, 445
BmrFI CCNGG 2 cut(s) 249, 782
BmrI ACTGGG 2 cut(s) 441, 596
BmsI GCATC 3 cut(s) 343, 448, 883
BmuI ACTGGG 2 cut(s) 441, 596
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 75
Bpu1102I GCTNAGC 1 cut(s) 633
BpuMI CCSGG 1 cut(s) 249
Bsa29I ATCGAT 1 cut(s) 335
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 228, 247, 402
BsaWI WCCGGW 1 cut(s) 681
Bsc4I CCNNNNNNNGG 5 cut(s) 16, 178, 248, 607, 608
Bse118I RCCGGY 3 cut(s) 190, 214, 271
Bse1I ACTGG 2 cut(s) 447, 602
Bse21I CCTNAGG 1 cut(s) 87
BseBI CCWGG 1 cut(s) 782
BseCI ATCGAT 1 cut(s) 335
BseDI CCNNGG 3 cut(s) 228, 247, 402
BseGI GGATG 3 cut(s) 777, 874, 880
BseLI CCNNNNNNNGG 5 cut(s) 16, 178, 248, 607, 608
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 2 cut(s) 447, 602
BseRI GAGGAG 2 cut(s) 310, 566
BseSI GKGCMC 2 cut(s) 188, 831
BseXI GCAGC 7 cut(s) 32, 35, 208, 367, 776, 852, 880
BsgI GTGCAG 2 cut(s) 18, 215
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 4 cut(s) 186, 389, 401, 600
BshVI ATCGAT 1 cut(s) 335
BsiHKAI GWGCWC 2 cut(s) 488, 831
BsiSI CCGG 5 cut(s) 191, 215, 249, 272, 682
BslFI GGGAC 2 cut(s) 456, 530
BslI CCNNNNNNNGG 5 cut(s) 16, 178, 248, 607, 608
BsmAI GTCTC 3 cut(s) 148, 533, 793
BsmBI CGTCTC 2 cut(s) 148, 793
BsmFI GGGAC 2 cut(s) 456, 530
BsnI GGCC 4 cut(s) 186, 389, 401, 600
Bsp120I GGGCCC 1 cut(s) 184
Bsp1286I GDGCHC 3 cut(s) 188, 488, 831
Bsp143I GATC 6 cut(s) 40, 94, 243, 406, 519, 655
Bsp1720I GCTNAGC 1 cut(s) 633
BspANI GGCC 4 cut(s) 186, 389, 401, 600
BspCNI CTCAG 1 cut(s) 493
BspDI ATCGAT 1 cut(s) 335
BspFNI CGCG 1 cut(s) 237
BspHI TCATGA 1 cut(s) 816
BspLI GGNNCC 3 cut(s) 186, 444, 445
BspMAI CTGCAG 1 cut(s) 870
BspPI GGATC 3 cut(s) 238, 414, 650
BsrBI CCGCTC 1 cut(s) 169
BsrFI RCCGGY 3 cut(s) 190, 214, 271
BsrI ACTGG 2 cut(s) 447, 602
BssAI RCCGGY 3 cut(s) 190, 214, 271
BssECI CCNNGG 3 cut(s) 228, 247, 402
BssMI GATC 6 cut(s) 40, 94, 243, 406, 519, 655
BssNI GRCGYC 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 782
Bst4CI ACNGT 2 cut(s) 544, 673
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 8 cut(s) 33, 188, 235, 273, 415, 577, 598, 639
BstDEI CTNAG 3 cut(s) 87, 480, 633
BstEII GGTNACC 1 cut(s) 677
BstF5I GGATG 3 cut(s) 777, 874, 880
BstFNI CGCG 1 cut(s) 237
BstH2I RGCGCY 1 cut(s) 866
BstHHI GCGC 3 cut(s) 239, 307, 865
BstKTI GATC 6 cut(s) 43, 97, 246, 409, 522, 658
BstMAI GTCTC 3 cut(s) 148, 533, 793
BstMBI GATC 6 cut(s) 40, 94, 243, 406, 519, 655
BstMWI GCNNNNNNNGC 8 cut(s) 32, 196, 208, 302, 386, 461, 638, 871
BstNI CCWGG 1 cut(s) 782
BstNSI RCATGY 1 cut(s) 424
BstPAI GACNNNNGTC 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 677
BstSCI CCNGG 2 cut(s) 247, 780
BstSFI CTRYAG 1 cut(s) 866
BstSLI GKGCMC 2 cut(s) 188, 831
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 7 cut(s) 32, 35, 208, 367, 776, 852, 880
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 2 cut(s) 406, 519
BstYI RGATCY 2 cut(s) 406, 519
Bsu15I ATCGAT 1 cut(s) 335
Bsu36I CCTNAGG 1 cut(s) 87
BsuRI GGCC 4 cut(s) 186, 389, 401, 600
BsuTUI ATCGAT 1 cut(s) 335
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 3 cut(s) 777, 874, 880
BtsI GCAGTG 1 cut(s) 655
BtsIMutI CAGTG 2 cut(s) 351, 655
Cac8I GCNNGC 8 cut(s) 33, 188, 235, 273, 415, 577, 598, 639
CciI TCATGA 1 cut(s) 816
CfoI GCGC 3 cut(s) 239, 307, 865
Cfr10I RCCGGY 3 cut(s) 190, 214, 271
Cfr13I GGNCC 5 cut(s) 135, 184, 185, 443, 599
ClaI ATCGAT 1 cut(s) 335
Csp6I GTAC 3 cut(s) 82, 494, 775
CviAII CATG 4 cut(s) 268, 421, 803, 817
CviQI GTAC 3 cut(s) 82, 494, 775
DdeI CTNAG 3 cut(s) 87, 480, 633
DpnI GATC 6 cut(s) 42, 96, 245, 408, 521, 657
DpnII GATC 6 cut(s) 40, 94, 243, 406, 519, 655
DraIII CACNNNGTG 1 cut(s) 16
EciI GGCGGA 3 cut(s) 102, 198, 290
Eco24I GRGCYC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 135, 443
Eco47III AGCGCT 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 749
Eco81I CCTNAGG 1 cut(s) 87
Eco91I GGTNACC 1 cut(s) 677
EcoO109I RGGNCCY 2 cut(s) 184, 443
EcoO65I GGTNACC 1 cut(s) 677
EcoRII CCWGG 1 cut(s) 780
EcoT38I GRGCYC 1 cut(s) 188
Esp3I CGTCTC 2 cut(s) 148, 793
FaeI CATG 4 cut(s) 271, 424, 806, 820
FaiI YATR 9 cut(s) 269, 422, 513, 619, 667, 702, 804, 818, 895
FaqI GGGAC 2 cut(s) 456, 530
FatI CATG 4 cut(s) 267, 420, 802, 816
FauI CCCGC 2 cut(s) 54, 195
FbaI TGATCA 1 cut(s) 40
FokI GGATG 3 cut(s) 764, 861, 867
FriOI GRGCYC 1 cut(s) 188
GlaI GCGC 3 cut(s) 238, 306, 864
HaeII RGCGCY 1 cut(s) 866
HaeIII GGCC 4 cut(s) 186, 389, 401, 600
HapII CCGG 5 cut(s) 191, 215, 249, 272, 682
HhaI GCGC 3 cut(s) 239, 307, 865
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 4 cut(s) 271, 424, 806, 820
Hin6I GCGC 3 cut(s) 237, 305, 863
HinP1I GCGC 3 cut(s) 237, 305, 863
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 2 cut(s) 278, 341
HpaII CCGG 5 cut(s) 191, 215, 249, 272, 682
HphI GGTGA 3 cut(s) 35, 689, 718
Hpy166II GTNNAC 3 cut(s) 223, 796, 829
Hpy188I TCNGA 7 cut(s) 67, 127, 157, 181, 243, 362, 483
Hpy188III TCNNGA 4 cut(s) 172, 328, 817, 857
Hpy8I GTNNAC 3 cut(s) 223, 796, 829
Hpy99I CGWCG 3 cut(s) 107, 110, 296
HpyAV CCTTC 4 cut(s) 370, 391, 497, 724
HpyCH4III ACNGT 2 cut(s) 544, 673
HpyCH4IV ACGT 2 cut(s) 105, 291
HpyCH4V TGCA 7 cut(s) 35, 196, 500, 641, 648, 829, 868
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 196, 208, 302, 386, 461, 638, 871
HpyF3I CTNAG 3 cut(s) 87, 480, 633
HpySE526I ACGT 2 cut(s) 105, 291
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 4 cut(s) 271, 424, 806, 820
HspAI GCGC 3 cut(s) 237, 305, 863
KflI GGGWCCC 1 cut(s) 443
KroI GCCGGC 1 cut(s) 271
KroNI GCCGGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 6 cut(s) 40, 94, 243, 406, 519, 655
LmnI GCTCC 6 cut(s) 82, 115, 174, 244, 553, 693
Lsp1109I GCAGC 7 cut(s) 32, 35, 208, 367, 776, 852, 880
LweI GCATC 3 cut(s) 343, 448, 883
MaeII ACGT 2 cut(s) 105, 291
MaeIII GTNAC 3 cut(s) 466, 650, 677
MalI GATC 6 cut(s) 42, 96, 245, 408, 521, 657
MbiI CCGCTC 1 cut(s) 169
MboI GATC 6 cut(s) 40, 94, 243, 406, 519, 655
MboII GAAGA 3 cut(s) 80, 529, 845
MflI RGATCY 2 cut(s) 406, 519
MhlI GDGCHC 3 cut(s) 188, 488, 831
MluCI AATT 3 cut(s) 474, 532, 612
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 159
MroNI GCCGGC 1 cut(s) 271
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 3 cut(s) 464, 727, 767
MspI CCGG 5 cut(s) 191, 215, 249, 272, 682
MspR9I CCNGG 2 cut(s) 249, 782
MvaI CCWGG 1 cut(s) 782
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 8 cut(s) 32, 196, 208, 302, 386, 461, 638, 871
NaeI GCCGGC 1 cut(s) 273
NciI CCSGG 1 cut(s) 249
NdeII GATC 6 cut(s) 40, 94, 243, 406, 519, 655
NgoMIV GCCGGC 1 cut(s) 271
NlaIII CATG 4 cut(s) 271, 424, 806, 820
NlaIV GGNNCC 3 cut(s) 186, 444, 445
NmeAIII GCCGAG 2 cut(s) 253, 427
NmuCI GTSAC 3 cut(s) 466, 650, 677
NspI RCATGY 1 cut(s) 424
PagI TCATGA 1 cut(s) 816
PciI ACATGT 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PdiI GCCGGC 1 cut(s) 273
PfeI GAWTC 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 16
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PpuMI RGGWCCY 1 cut(s) 443
PscI ACATGT 1 cut(s) 420
PshAI GACNNNNGTC 1 cut(s) 140
PsiI TTATAA 1 cut(s) 702
Psp5II RGGWCCY 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 780
PspEI GGTNACC 1 cut(s) 677
PspGI CCWGG 1 cut(s) 780
PspN4I GGNNCC 3 cut(s) 186, 444, 445
PspOMI GGGCCC 1 cut(s) 184
PspPI GGNCC 5 cut(s) 135, 184, 185, 443, 599
PspPPI RGGWCCY 1 cut(s) 443
PstI CTGCAG 1 cut(s) 870
PsuI RGATCY 2 cut(s) 406, 519
PvuII CAGCTG 2 cut(s) 727, 767
RsaI GTAC 3 cut(s) 83, 495, 776
RsaNI GTAC 3 cut(s) 82, 494, 775
RseI CAYNNNNRTG 1 cut(s) 266
Sau3AI GATC 6 cut(s) 40, 94, 243, 406, 519, 655
Sau96I GGNCC 5 cut(s) 135, 184, 185, 443, 599
ScaI AGTACT 1 cut(s) 495
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 249, 782
SduI GDGCHC 3 cut(s) 188, 488, 831
SfaNI GCATC 3 cut(s) 343, 448, 883
SfcI CTRYAG 1 cut(s) 866
SgrAI CRCCGGYG 2 cut(s) 190, 214
SinI GGWCC 2 cut(s) 135, 443
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 3 cut(s) 474, 532, 612
StyD4I CCNGG 2 cut(s) 247, 780
TaaI ACNGT 2 cut(s) 544, 673
TaiI ACGT 2 cut(s) 108, 294
TaqI TCGA 3 cut(s) 93, 335, 526
TasI AATT 3 cut(s) 474, 532, 612
TatI WGTACW 2 cut(s) 493, 774
TauI GCSGC 6 cut(s) 202, 214, 260, 310, 464, 874
TfiI GAWTC 1 cut(s) 341
TscAI CASTG 2 cut(s) 351, 655
TseFI GTSAC 3 cut(s) 466, 650, 677
TseI GCWGC 7 cut(s) 20, 23, 196, 380, 764, 865, 868
Tsp45I GTSAC 3 cut(s) 466, 650, 677
TspDTI ATGAA 2 cut(s) 654, 805
TspGWI ACGGA 3 cut(s) 69, 130, 313
TspRI CASTG 2 cut(s) 351, 655
Van91I CCANNNNNTGG 1 cut(s) 16
VneI GTGCAC 1 cut(s) 827
VpaK11BI GGWCC 2 cut(s) 135, 443
XapI RAATTY 1 cut(s) 612
XceI RCATGY 1 cut(s) 424
ZraI GACGTC 1 cut(s) 106
ZrmI AGTACT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.