FvH4_6g00670

trigalactosyldiacylglycerol 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014402
Physical Location & Seq
Forward (+)
387123 .. 392153
5031 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00670.t2

Sequence Viewer

Length: 1041 bp
ATGGTTTCTCTGGCGAATTCAGTGTTGTTTCCTGTGACTACATCCAATGGCATTTCTGGGCCGGTTAGATTAGCAAAGACGGTCAGTTTGTGTAGCTATAAGCAGAAGAGAGAGCCCAGAAACATTGTGTGTGCTTGTATGGCGCCTCCCCATAACTTGAGGAGCGATGAATCCTTTGCAGCTAAATTCGAGGATTCATTGAAATCAGAGGATTTAACCACAGTAAGAGAGCCCGAGGATGACTCTGATGTTCTGATTGAGTGCAGAGATGTCTACAAGTCATTTGGTGAGAAGGATATATTGAGAGGTGTGAGCTTCAAGATTAGACATGGCGAAGCAGTTGGAATAATTGGTCCTTCTGGCACGGGAAAATCTACAGTTTTAAAGATTATGGCAGGGCTTCTTGCGCCTGACAAGGGAGAGGTATACATTCGAGGTCGAAAGAGAGCTGGTTTGATAAGCGACGAAGAGTTATCTGGTCTTCGAATTGGATTGGTGTTTCAGAGTGCTGCACTTTTTGATTCGTTGACTGTTCGTGAAAATGTTGGTTTTCTTCTGTATGAAAATTCAAGCATGCCCGAGGAACAGATTTCAAAGCTCGTGACTGCTAGCTTAGCTGCTGTTGGGTTAAAGGGAGTTGAGGATCGATTACCTTCAGAGCTGTCGGGTGGTATGAAGAAAAGAGTTGCATTGGCTCGATCTATAATATGTGATACGACAAAGGAATCCATAGAGCCAGAGGTGCTCCTATATGATGAACCAACAGCTGGACTTGATCCGATTGCATCTACTGTTGTAGAAGACCTCATCCGCTTTGTCCATACAGAAGGGCAGGCCGCATCAGAGAAGCCTGGGCAGATTGCATCTTATGTTGTTGTAACTCACCAACATAGCACCATCAGACGAGCTGTTGACAGGTTATTGTTTCTGTATGAGGGCAAGATAGTCTGGCAAGGAATGACTGACGAATTCACAACCTCAACAAATCCAATCGTTCAACAGTTTGCATCTGGGAACTTGGATGGCCCAATTAAATACTAG

Protein Analysis

347

Amino Acids

37.7

Weight (kDa)

5.57

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 101 - 256 1.2e-30 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65410
fragaria_vesca FvH4_6g00670 FvH4_6g00670
malus_domestica MD04G1243700.v1.1 MD12G1259300.v1.1
prunus_persica Prupe.6G359900_v2.0.a1
pyrus_communis pycom04g21440 pycom12g23770
rosa_chinensis RchiOBHm_Chr3g0448111
rosa_laevigata RLG00000025946
rosa_multiflora Rmu_sc0005969.1_g000027
rosa_roxburghii Rroxscaffold_6G00426240
rosa_rugosa Rorug02G0608200
rosa_samantha Rh3AG007700 Rh3CG007400 Rh3DG007900
rosa_wichuraiana Rw3G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 142
AccB7I CCANNNNNTGG 1 cut(s) 767
AccI GTMKAC 2 cut(s) 273, 426
AciI CCGC 2 cut(s) 811, 837
AclWI GGATC 2 cut(s) 651, 770
AcsI RAATTY 4 cut(s) 16, 185, 565, 968
AcuI CTGAAG 1 cut(s) 639
AcyI GRCGYC 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 416, 767
AgsI TTSAA 5 cut(s) 202, 319, 570, 594, 998
AjnI CCWGG 1 cut(s) 850
Alw21I GWGCWC 1 cut(s) 747
AlwI GGATC 2 cut(s) 651, 770
Ama87I CYCGRG 2 cut(s) 233, 578
AoxI GGCC 3 cut(s) 59, 834, 1024
ApeKI GCWGC 3 cut(s) 179, 509, 617
ApoI RAATTY 4 cut(s) 16, 185, 565, 968
ArsI GACNNNNNNTTYG 2 cut(s) 70, 102
AspLEI GCGC 2 cut(s) 145, 409
AspS9I GGNCC 3 cut(s) 59, 353, 1025
AsuHPI GGTGA 2 cut(s) 299, 875
AsuII TTCGAA 1 cut(s) 484
AsuNHI GCTAGC 1 cut(s) 608
AvaI CYCGRG 2 cut(s) 233, 578
AvaII GGWCC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 142
BanII GRGCYC 2 cut(s) 117, 234
BauI CACGAG 1 cut(s) 599
BbsI GAAGAC 2 cut(s) 473, 807
Bbv12I GWGCWC 1 cut(s) 747
BbvI GCAGC 3 cut(s) 191, 496, 604
BccI CCATC 2 cut(s) 905, 1016
BciT130I CCWGG 1 cut(s) 852
BfaI CTAG 2 cut(s) 609, 1039
BfmI CTRYAG 1 cut(s) 375
BfoI RGCGCY 1 cut(s) 146
BisI GCNGC 4 cut(s) 180, 510, 618, 837
BlpI GCTNAGC 1 cut(s) 613
BlsI GCNGC 4 cut(s) 181, 511, 619, 838
Bme1390I CCNGG 1 cut(s) 852
Bme18I GGWCC 1 cut(s) 353
BmeT110I CYCGRG 2 cut(s) 233, 578
BmgT120I GGNCC 3 cut(s) 59, 353, 1025
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 852
BmsI GCATC 4 cut(s) 794, 848, 872, 1016
BmtI GCTAGC 1 cut(s) 612
BpiI GAAGAC 2 cut(s) 473, 807
BplI GAGNNNNNCTC 2 cut(s) 227, 259
Bpu1102I GCTNAGC 1 cut(s) 613
Bpu14I TTCGAA 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 178
Bsa29I ATCGAT 1 cut(s) 646
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 3 cut(s) 234, 579, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 416, 767
Bse118I RCCGGY 1 cut(s) 61
BseBI CCWGG 1 cut(s) 852
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 3 cut(s) 234, 579, 851
BseGI GGATG 4 cut(s) 41, 244, 807, 1027
BseLI CCNNNNNNNGG 2 cut(s) 416, 767
BseRI GAGGAG 1 cut(s) 175
BseXI GCAGC 3 cut(s) 191, 496, 604
BsgI GTGCAG 2 cut(s) 283, 495
BshFI GGCC 3 cut(s) 61, 836, 1026
BshNI GGYRCC 1 cut(s) 142
BshVI ATCGAT 1 cut(s) 646
BsiHKAI GWGCWC 1 cut(s) 747
BsiHKCI CYCGRG 2 cut(s) 233, 578
BsiSI CCGG 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 416, 767
BsnI GGCC 3 cut(s) 61, 836, 1026
BsoBI CYCGRG 2 cut(s) 233, 578
Bsp119I TTCGAA 1 cut(s) 484
Bsp1286I GDGCHC 3 cut(s) 117, 234, 747
Bsp143I GATC 3 cut(s) 643, 698, 775
Bsp1720I GCTNAGC 1 cut(s) 613
BspACI CCGC 2 cut(s) 811, 837
BspANI GGCC 3 cut(s) 61, 836, 1026
BspDI ATCGAT 1 cut(s) 646
BspLI GGNNCC 1 cut(s) 144
BspOI GCTAGC 1 cut(s) 612
BspPI GGATC 2 cut(s) 651, 770
BspT104I TTCGAA 1 cut(s) 484
BspT107I GGYRCC 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 61
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 3 cut(s) 234, 579, 851
BssMI GATC 3 cut(s) 643, 698, 775
BssNAI GTATAC 1 cut(s) 427
BssNI GRCGYC 1 cut(s) 143
BssSI CACGAG 1 cut(s) 599
Bst1107I GTATAC 1 cut(s) 427
Bst2BI CACGAG 1 cut(s) 599
Bst2UI CCWGG 1 cut(s) 852
Bst4CI ACNGT 6 cut(s) 82, 223, 379, 532, 793, 1002
Bst6I CTCTTC 2 cut(s) 101, 462
BstACI GRCGYC 1 cut(s) 143
BstBI TTCGAA 1 cut(s) 484
BstC8I GCNNGC 3 cut(s) 575, 610, 834
BstDEI CTNAG 1 cut(s) 613
BstF5I GGATG 4 cut(s) 41, 244, 807, 1027
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 2 cut(s) 145, 409
BstKTI GATC 3 cut(s) 646, 701, 778
BstMBI GATC 3 cut(s) 643, 698, 775
BstMWI GCNNNNNNNGC 4 cut(s) 140, 406, 614, 742
BstNI CCWGG 1 cut(s) 852
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 1 cut(s) 850
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 3 cut(s) 191, 496, 604
BstV2I GAAGAC 2 cut(s) 473, 807
BstZ17I GTATAC 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 646
BsuRI GGCC 3 cut(s) 61, 836, 1026
BsuTUI ATCGAT 1 cut(s) 646
BtgZI GCGATG 1 cut(s) 180
BtsCI GGATG 4 cut(s) 41, 244, 807, 1027
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 3 cut(s) 575, 610, 834
CfoI GCGC 2 cut(s) 145, 409
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 3 cut(s) 59, 353, 1025
ClaI ATCGAT 1 cut(s) 646
CviAII CATG 2 cut(s) 329, 574
DdeI CTNAG 1 cut(s) 613
DinI GGCGCC 1 cut(s) 144
DpnI GATC 3 cut(s) 645, 700, 777
DpnII GATC 3 cut(s) 643, 698, 775
DraI TTTAAA 1 cut(s) 384
Eam1104I CTCTTC 2 cut(s) 101, 462
EarI CTCTTC 2 cut(s) 101, 462
Eco24I GRGCYC 2 cut(s) 117, 234
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 2 cut(s) 233, 578
EcoRI GAATTC 2 cut(s) 16, 968
EcoRII CCWGG 1 cut(s) 850
EcoT38I GRGCYC 2 cut(s) 117, 234
EgeI GGCGCC 1 cut(s) 144
EheI GGCGCC 1 cut(s) 144
FaeI CATG 2 cut(s) 332, 577
FatI CATG 2 cut(s) 328, 573
FblI GTMKAC 2 cut(s) 273, 426
Fnu4HI GCNGC 4 cut(s) 180, 510, 618, 837
FokI GGATG 4 cut(s) 28, 251, 794, 1034
FriOI GRGCYC 2 cut(s) 117, 234
Fsp4HI GCNGC 4 cut(s) 180, 510, 618, 837
FspBI CTAG 2 cut(s) 609, 1039
GlaI GCGC 2 cut(s) 144, 408
GluI GCNGC 4 cut(s) 180, 510, 618, 837
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 3 cut(s) 61, 836, 1026
HapII CCGG 1 cut(s) 62
HhaI GCGC 2 cut(s) 145, 409
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 2 cut(s) 332, 577
Hin6I GCGC 2 cut(s) 143, 407
HinP1I GCGC 2 cut(s) 143, 407
HincII GTYRAC 2 cut(s) 528, 913
HindII GTYRAC 2 cut(s) 528, 913
HinfI GANTC 5 cut(s) 170, 194, 242, 521, 725
HpaII CCGG 1 cut(s) 62
HphI GGTGA 2 cut(s) 299, 875
Hpy166II GTNNAC 4 cut(s) 274, 427, 528, 913
Hpy188I TCNGA 8 cut(s) 208, 247, 255, 504, 658, 780, 844, 902
Hpy188III TCNNGA 3 cut(s) 319, 536, 601
Hpy8I GTNNAC 4 cut(s) 274, 427, 528, 913
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 4 cut(s) 286, 366, 663, 821
HpyCH4III ACNGT 6 cut(s) 82, 223, 379, 532, 793, 1002
HpyCH4V TGCA 7 cut(s) 179, 264, 512, 689, 785, 863, 1007
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 406, 614, 742
HpyF3I CTNAG 1 cut(s) 613
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 2 cut(s) 332, 577
HspAI GCGC 2 cut(s) 143, 407
KasI GGCGCC 1 cut(s) 142
Kzo9I GATC 3 cut(s) 643, 698, 775
LmnI GCTCC 2 cut(s) 162, 750
Lsp1109I GCAGC 3 cut(s) 191, 496, 604
LweI GCATC 4 cut(s) 794, 848, 872, 1016
MaeI CTAG 2 cut(s) 609, 1039
MaeIII GTNAC 3 cut(s) 34, 601, 877
MalI GATC 3 cut(s) 645, 700, 777
MboI GATC 3 cut(s) 643, 698, 775
MboII GAAGA 6 cut(s) 118, 473, 479, 545, 688, 812
MhlI GDGCHC 3 cut(s) 117, 234, 747
MluCI AATT 7 cut(s) 16, 185, 348, 486, 565, 968, 1029
Mly113I GGCGCC 1 cut(s) 143
MlyI GAGTC 1 cut(s) 236
MmeI TCCRAC 1 cut(s) 322
MseI TTAA 4 cut(s) 215, 383, 629, 1032
MspA1I CMGCKG 1 cut(s) 767
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 852
MvaI CCWGG 1 cut(s) 852
MwoI GCNNNNNNNGC 4 cut(s) 140, 406, 614, 742
NarI GGCGCC 1 cut(s) 143
NdeII GATC 3 cut(s) 643, 698, 775
NheI GCTAGC 1 cut(s) 608
NlaIII CATG 2 cut(s) 332, 577
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 34, 601
NspI RCATGY 1 cut(s) 577
NspV TTCGAA 1 cut(s) 484
PaeI GCATGC 1 cut(s) 577
PfeI GAWTC 4 cut(s) 170, 194, 521, 725
PflMI CCANNNNNTGG 1 cut(s) 767
PkrI GCNGC 4 cut(s) 181, 511, 619, 838
PleI GAGTC 1 cut(s) 236
PluTI GGCGCC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 850
PspGI CCWGG 1 cut(s) 850
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 3 cut(s) 59, 353, 1025
PvuII CAGCTG 1 cut(s) 767
SaqAI TTAA 4 cut(s) 215, 383, 629, 1032
SatI GCNGC 4 cut(s) 180, 510, 618, 837
Sau3AI GATC 3 cut(s) 643, 698, 775
Sau96I GGNCC 3 cut(s) 59, 353, 1025
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 1 cut(s) 852
SduI GDGCHC 3 cut(s) 117, 234, 747
SfaNI GCATC 4 cut(s) 794, 848, 872, 1016
SfcI CTRYAG 1 cut(s) 375
SfoI GGCGCC 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 484
SinI GGWCC 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SphI GCATGC 1 cut(s) 577
Sse9I AATT 7 cut(s) 16, 185, 348, 486, 565, 968, 1029
SsiI CCGC 2 cut(s) 811, 837
SspDI GGCGCC 1 cut(s) 142
SspMI CTAG 2 cut(s) 609, 1039
StyD4I CCNGG 1 cut(s) 850
TaaI ACNGT 6 cut(s) 82, 223, 379, 532, 793, 1002
TaqI TCGA 6 cut(s) 189, 433, 439, 484, 646, 697
TasI AATT 7 cut(s) 16, 185, 348, 486, 565, 968, 1029
TauI GCSGC 1 cut(s) 839
TfiI GAWTC 4 cut(s) 170, 194, 521, 725
Tru1I TTAA 4 cut(s) 215, 383, 629, 1032
Tru9I TTAA 4 cut(s) 215, 383, 629, 1032
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 2 cut(s) 34, 601
TseI GCWGC 3 cut(s) 179, 509, 617
Tsp45I GTSAC 2 cut(s) 34, 601
TspDTI ATGAA 5 cut(s) 183, 186, 576, 689, 771
TspRI CASTG 1 cut(s) 27
Van91I CCANNNNNTGG 1 cut(s) 767
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 4 cut(s) 16, 185, 565, 968
XceI RCATGY 1 cut(s) 577
XmiI GTMKAC 2 cut(s) 273, 426
XspI CTAG 2 cut(s) 609, 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.