MD12G1259300.v1.1

trigalactosyldiacylglycerol 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014402
Physical Location & Seq
Reverse (-)
32575473 .. 32582055
6583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1259300.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGTTTTCTCTGTCGAATTCAGTGTTGTTTCCTCTGGCGACATCCAGCGGCTTCACATCCAAAGGTTTTTCTCGGCCCGTTCGATTTGCCAGGACGATCAATTGGCATAGTAGTGACAAGCAAAAGGAAGAGCCCAGAAACGTTTTGTGCGCTTGTATGGCGCCTCACCCGAACCTGCGCAGCGATGAATCCTTTGCTACAAAATTTGAGGATTCATTTAAATCAGAGGATTTAAGTAGAGTTCAGGAGCCTGAGGGTGATTCGGATGTTCTGATCGAGTGTAGAGACGTCTACAAATCGTTTGGCGAGAAGAATATATTGAGAGGTGTGAGCTTCAAGATTAGGCATGGCGAAGCTGTTGGAATAATTGGGCCTTCTGGCACTGGAAAATCTACAGTTCTGAAGATAATGGCAGGGCTTCTTGCTCCTGACAAGGGAGAGGTGTACATTCGAGGTCGAAAGAGAGTTGGTTTGATCAGCGATGATGAGATATCCGGTCTTCGGATTGGATTGGTGTTTCAGAGCGCTGCACTTTTTGATTCTTTGACTGTTCGTGAAAATGTCGGTTTTCTTCTGTACGAAAATTCGAGCATGCCCGAGGATCAAATTGCAAAGCTTGTGACTGAGAGCTTGGCCGCAGTCGGGTTAAAGGGAGTTGAAGATCGATTACCTTCGGAGCTGTCTGGTGGAATGAAGAAACGAGTTGCTCTGGCTCGATCCATAATTTATGATACCACAAAGGAAGCGATAGAGCCAGAGGTGCTCTTATATGATGAGCCAACAGCTGGACTTGATCCTATTGCATCTACTGTGGTAGAAGACCTCATCCGCTCTGTTCATACAAAAGGTCAGGATGCAGTCGAGAAACCTGGGCAGATCGCATCTTATGTTGTTGTTACTCACCAACATAGTACCATCAGACGAGCTGTCGACAGGTTGTTGTTTCTCTACGAGGGCGAGATAGTCTGGCAAGGAATGACTCATGAATTCACAACCTCAACAAATCCAATCGTTCAACAGTTTGCATCCGGGAACTTGGACGGTCCGATTAAATATTAG

Protein Analysis

353

Amino Acids

38.72

Weight (kDa)

5.75

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 107 - 262 7e-30 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65410
fragaria_vesca FvH4_6g00670 FvH4_6g00670
malus_domestica MD04G1243700.v1.1 MD12G1259300.v1.1
prunus_persica Prupe.6G359900_v2.0.a1
pyrus_communis pycom04g21440 pycom12g23770
rosa_chinensis RchiOBHm_Chr3g0448111
rosa_laevigata RLG00000025946
rosa_multiflora Rmu_sc0005969.1_g000027
rosa_roxburghii Rroxscaffold_6G00426240
rosa_rugosa Rorug02G0608200
rosa_samantha Rh3AG007700 Rh3CG007400 Rh3DG007900
rosa_wichuraiana Rw3G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
Acc16I TGCGCA 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 160
AccB7I CCANNNNNTGG 1 cut(s) 785
AccBSI CCGCTC 1 cut(s) 831
AccI GTMKAC 2 cut(s) 291, 930
AciI CCGC 3 cut(s) 48, 636, 829
AclI AACGTT 1 cut(s) 141
AclWI GGATC 3 cut(s) 609, 711, 788
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 4 cut(s) 16, 203, 583, 986
AcuI CTGAAG 1 cut(s) 422
AcyI GRCGYC 2 cut(s) 161, 288
AfaI GTAC 3 cut(s) 446, 578, 913
AfeI AGCGCT 1 cut(s) 526
AfiI CCNNNNNNNGG 4 cut(s) 434, 501, 642, 785
AgsI TTSAA 3 cut(s) 337, 659, 1016
AhdI GACNNNNNGTC 1 cut(s) 926
AjnI CCWGG 2 cut(s) 89, 868
AluBI AGCT 7 cut(s) 333, 356, 616, 630, 679, 785, 926
AluI AGCT 7 cut(s) 333, 356, 616, 630, 679, 785, 926
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 3 cut(s) 609, 711, 788
Ama87I CYCGRG 1 cut(s) 596
Aor51HI AGCGCT 1 cut(s) 526
AoxI GGCC 3 cut(s) 74, 371, 633
ApeKI GCWGC 2 cut(s) 180, 527
ApoI RAATTY 4 cut(s) 16, 203, 583, 986
AspLEI GCGC 4 cut(s) 152, 163, 180, 527
AspS9I GGNCC 3 cut(s) 75, 371, 1043
AsuC2I CCSGG 1 cut(s) 1030
AsuHPI GGTGA 3 cut(s) 158, 269, 893
AvaI CYCGRG 1 cut(s) 596
AvaII GGWCC 1 cut(s) 1043
AxyI CCTNAGG 1 cut(s) 252
BanI GGYRCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 135
BarI GAAGNNNNNNTAC 2 cut(s) 651, 683
BbsI GAAGAC 2 cut(s) 491, 825
Bbv12I GWGCWC 1 cut(s) 765
BbvI GCAGC 2 cut(s) 192, 514
BccI CCATC 1 cut(s) 923
BciT130I CCWGG 2 cut(s) 91, 870
BclI TGATCA 1 cut(s) 474
BcnI CCSGG 1 cut(s) 1030
BcoDI GTCTC 1 cut(s) 279
BfmI CTRYAG 1 cut(s) 393
BfoI RGCGCY 2 cut(s) 164, 528
BfuAI ACCTGC 1 cut(s) 183
BisI GCNGC 4 cut(s) 49, 181, 528, 636
BlsI GCNGC 4 cut(s) 50, 182, 529, 637
Bme1390I CCNGG 3 cut(s) 91, 870, 1030
Bme18I GGWCC 1 cut(s) 1043
BmeRI GACNNNNNGTC 1 cut(s) 926
BmeT110I CYCGRG 1 cut(s) 596
BmgT120I GGNCC 3 cut(s) 75, 371, 1043
BmiI GGNNCC 2 cut(s) 162, 249
BmrFI CCNGG 3 cut(s) 91, 870, 1030
BmsI GCATC 4 cut(s) 812, 844, 890, 1034
BpiI GAAGAC 2 cut(s) 491, 825
BpuMI CCSGG 1 cut(s) 1030
Bsa29I ATCGAT 1 cut(s) 664
BsaHI GRCGYC 2 cut(s) 161, 288
BsaJI CCNNGG 2 cut(s) 597, 869
BsaWI WCCGGW 1 cut(s) 494
Bsc4I CCNNNNNNNGG 4 cut(s) 434, 501, 642, 785
Bse1I ACTGG 1 cut(s) 388
Bse21I CCTNAGG 1 cut(s) 252
BseBI CCWGG 2 cut(s) 91, 870
BseCI ATCGAT 1 cut(s) 664
BseDI CCNNGG 2 cut(s) 597, 869
BseGI GGATG 6 cut(s) 41, 56, 271, 825, 859, 1025
BseLI CCNNNNNNNGG 4 cut(s) 434, 501, 642, 785
BseMII CTCAG 2 cut(s) 243, 615
BseNI ACTGG 1 cut(s) 388
BseXI GCAGC 2 cut(s) 192, 514
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 3 cut(s) 76, 373, 635
BshNI GGYRCC 1 cut(s) 160
BshVI ATCGAT 1 cut(s) 664
BsiHKAI GWGCWC 1 cut(s) 765
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 2 cut(s) 495, 1029
BslI CCNNNNNNNGG 4 cut(s) 434, 501, 642, 785
BsmAI GTCTC 1 cut(s) 279
BsmBI CGTCTC 1 cut(s) 279
BsnI GGCC 3 cut(s) 76, 373, 635
BsoBI CYCGRG 1 cut(s) 596
Bsp1286I GDGCHC 2 cut(s) 135, 765
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
BspACI CCGC 3 cut(s) 48, 636, 829
BspANI GGCC 3 cut(s) 76, 373, 635
BspCNI CTCAG 2 cut(s) 244, 616
BspDI ATCGAT 1 cut(s) 664
BspHI TCATGA 1 cut(s) 982
BspLI GGNNCC 2 cut(s) 162, 249
BspMI ACCTGC 1 cut(s) 183
BspPI GGATC 3 cut(s) 609, 711, 788
BspQI GCTCTTC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 160
BsrBI CCGCTC 1 cut(s) 831
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 1 cut(s) 388
BssECI CCNNGG 2 cut(s) 597, 869
BssMI GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
BssNI GRCGYC 2 cut(s) 161, 288
Bst2UI CCWGG 2 cut(s) 91, 870
Bst4CI ACNGT 5 cut(s) 397, 550, 811, 1020, 1043
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 2 cut(s) 161, 288
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 593
BstDEI CTNAG 2 cut(s) 252, 624
BstF5I GGATG 6 cut(s) 41, 56, 271, 825, 859, 1025
BstH2I RGCGCY 2 cut(s) 164, 528
BstHHI GCGC 4 cut(s) 152, 163, 180, 527
BstKTI GATC 8 cut(s) 99, 276, 477, 604, 664, 719, 796, 879
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
BstMWI GCNNNNNNNGC 2 cut(s) 158, 760
BstNI CCWGG 2 cut(s) 91, 870
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 3 cut(s) 89, 868, 1028
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 2 cut(s) 192, 514
BstV2I GAAGAC 2 cut(s) 491, 825
Bsu15I ATCGAT 1 cut(s) 664
Bsu36I CCTNAGG 1 cut(s) 252
BsuRI GGCC 3 cut(s) 76, 373, 635
BsuTUI ATCGAT 1 cut(s) 664
BtgZI GCGATG 2 cut(s) 198, 495
BtsCI GGATG 6 cut(s) 41, 56, 271, 825, 859, 1025
BtsIMutI CAGTG 2 cut(s) 27, 381
BveI ACCTGC 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 593
CciI TCATGA 1 cut(s) 982
CfoI GCGC 4 cut(s) 152, 163, 180, 527
Cfr13I GGNCC 3 cut(s) 75, 371, 1043
ClaI ATCGAT 1 cut(s) 664
CpoI CGGWCCG 1 cut(s) 1043
Csp6I GTAC 3 cut(s) 445, 577, 912
CspI CGGWCCG 1 cut(s) 1043
CviAII CATG 3 cut(s) 347, 592, 983
CviQI GTAC 3 cut(s) 445, 577, 912
DdeI CTNAG 2 cut(s) 252, 624
DinI GGCGCC 1 cut(s) 162
DpnI GATC 8 cut(s) 98, 275, 476, 603, 663, 718, 795, 878
DpnII GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
DraI TTTAAA 1 cut(s) 220
DriI GACNNNNNGTC 1 cut(s) 926
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 1 cut(s) 123
Eam1105I GACNNNNNGTC 1 cut(s) 926
EarI CTCTTC 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 1043
Eco47III AGCGCT 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 422
Eco81I CCTNAGG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 596
EcoRI GAATTC 2 cut(s) 16, 986
EcoRII CCWGG 2 cut(s) 89, 868
EcoRV GATATC 1 cut(s) 492
EcoT38I GRGCYC 1 cut(s) 135
EgeI GGCGCC 1 cut(s) 162
EheI GGCGCC 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 3 cut(s) 350, 595, 986
FatI CATG 3 cut(s) 346, 591, 982
FbaI TGATCA 1 cut(s) 474
FblI GTMKAC 2 cut(s) 291, 930
Fnu4HI GCNGC 4 cut(s) 49, 181, 528, 636
FokI GGATG 6 cut(s) 28, 43, 278, 812, 866, 1012
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 4 cut(s) 49, 181, 528, 636
FspI TGCGCA 1 cut(s) 179
GlaI GCGC 4 cut(s) 151, 162, 179, 526
GluI GCNGC 4 cut(s) 49, 181, 528, 636
HaeII RGCGCY 2 cut(s) 164, 528
HaeIII GGCC 3 cut(s) 76, 373, 635
HapII CCGG 2 cut(s) 495, 1029
HhaI GCGC 4 cut(s) 152, 163, 180, 527
Hin1I GRCGYC 2 cut(s) 161, 288
Hin1II CATG 3 cut(s) 350, 595, 986
Hin6I GCGC 4 cut(s) 150, 161, 178, 525
HinP1I GCGC 4 cut(s) 150, 161, 178, 525
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 5 cut(s) 188, 212, 260, 539, 979
HpaII CCGG 2 cut(s) 495, 1029
HphI GGTGA 3 cut(s) 158, 269, 893
Hpy166II GTNNAC 3 cut(s) 292, 445, 931
Hpy188I TCNGA 9 cut(s) 226, 265, 273, 402, 504, 522, 676, 920, 1047
Hpy188III TCNNGA 7 cut(s) 245, 337, 428, 554, 851, 862, 983
Hpy8I GTNNAC 3 cut(s) 292, 445, 931
HpyAV CCTTC 2 cut(s) 384, 681
HpyCH4III ACNGT 5 cut(s) 397, 550, 811, 1020, 1043
HpyCH4IV ACGT 2 cut(s) 141, 288
HpyCH4V TGCA 5 cut(s) 530, 611, 803, 857, 1025
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 760
HpyF3I CTNAG 2 cut(s) 252, 624
HpySE526I ACGT 2 cut(s) 141, 288
Hsp92I GRCGYC 2 cut(s) 161, 288
Hsp92II CATG 3 cut(s) 350, 595, 986
HspAI GCGC 4 cut(s) 150, 161, 178, 525
KasI GGCGCC 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 3 cut(s) 247, 430, 676
Lsp1109I GCAGC 2 cut(s) 192, 514
LweI GCATC 4 cut(s) 812, 844, 890, 1034
MaeII ACGT 2 cut(s) 141, 288
MaeIII GTNAC 3 cut(s) 113, 619, 895
MalI GATC 8 cut(s) 98, 275, 476, 603, 663, 718, 795, 878
MbiI CCGCTC 1 cut(s) 831
MboI GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
MboII GAAGA 8 cut(s) 140, 322, 415, 491, 563, 671, 706, 830
MfeI CAATTG 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 135, 765
MluCI AATT 8 cut(s) 16, 100, 203, 366, 583, 606, 723, 986
Mly113I GGCGCC 1 cut(s) 161
MlyI GAGTC 1 cut(s) 973
MmeI TCCRAC 1 cut(s) 340
MseI TTAA 4 cut(s) 219, 233, 647, 1050
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 2 cut(s) 48, 785
MspI CCGG 2 cut(s) 495, 1029
MspR9I CCNGG 3 cut(s) 91, 870, 1030
MunI CAATTG 1 cut(s) 100
MvaI CCWGG 2 cut(s) 91, 870
MwoI GCNNNNNNNGC 2 cut(s) 158, 760
NarI GGCGCC 1 cut(s) 161
NciI CCSGG 1 cut(s) 1030
NdeII GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
NlaIII CATG 3 cut(s) 350, 595, 986
NlaIV GGNNCC 2 cut(s) 162, 249
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 113, 619
NsbI TGCGCA 1 cut(s) 179
NspI RCATGY 1 cut(s) 595
PaeI GCATGC 1 cut(s) 595
PagI TCATGA 1 cut(s) 982
PciSI GCTCTTC 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PfeI GAWTC 4 cut(s) 188, 212, 260, 539
PflMI CCANNNNNTGG 1 cut(s) 785
PfoI TCCNGGA 1 cut(s) 1028
PkrI GCNGC 4 cut(s) 50, 182, 529, 637
PleI GAGTC 1 cut(s) 973
PluTI GGCGCC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 973
Psp1406I AACGTT 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 89, 868
PspGI CCWGG 2 cut(s) 89, 868
PspN4I GGNNCC 2 cut(s) 162, 249
PspPI GGNCC 3 cut(s) 75, 371, 1043
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 3 cut(s) 446, 578, 913
RsaNI GTAC 3 cut(s) 445, 577, 912
RseI CAYNNNNRTG 1 cut(s) 111
Rsr2I CGGWCCG 1 cut(s) 1043
RsrII CGGWCCG 1 cut(s) 1043
SalI GTCGAC 1 cut(s) 929
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 4 cut(s) 219, 233, 647, 1050
SatI GCNGC 4 cut(s) 49, 181, 528, 636
Sau3AI GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
Sau96I GGNCC 3 cut(s) 75, 371, 1043
SchI GAGTC 1 cut(s) 973
ScrFI CCNGG 3 cut(s) 91, 870, 1030
SduI GDGCHC 2 cut(s) 135, 765
SfaNI GCATC 4 cut(s) 812, 844, 890, 1034
SfcI CTRYAG 1 cut(s) 393
SfoI GGCGCC 1 cut(s) 162
SinI GGWCC 1 cut(s) 1043
SmiI ATTTAAAT 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 111
SphI GCATGC 1 cut(s) 595
Sse9I AATT 8 cut(s) 16, 100, 203, 366, 583, 606, 723, 986
SsiI CCGC 3 cut(s) 48, 636, 829
SspDI GGCGCC 1 cut(s) 160
SspI AATATT 1 cut(s) 1055
StyD4I CCNGG 3 cut(s) 89, 868, 1028
SwaI ATTTAAAT 1 cut(s) 220
TaaI ACNGT 5 cut(s) 397, 550, 811, 1020, 1043
TaiI ACGT 2 cut(s) 144, 291
TasI AATT 8 cut(s) 16, 100, 203, 366, 583, 606, 723, 986
TatI WGTACW 1 cut(s) 444
TauI GCSGC 2 cut(s) 51, 638
TfiI GAWTC 4 cut(s) 188, 212, 260, 539
Tru1I TTAA 4 cut(s) 219, 233, 647, 1050
Tru9I TTAA 4 cut(s) 219, 233, 647, 1050
TscAI CASTG 2 cut(s) 27, 388
TseFI GTSAC 2 cut(s) 113, 619
TseI GCWGC 2 cut(s) 180, 527
Tsp45I GTSAC 2 cut(s) 113, 619
TspDTI ATGAA 5 cut(s) 201, 204, 707, 827, 999
TspRI CASTG 2 cut(s) 27, 388
Van91I CCANNNNNTGG 1 cut(s) 785
VpaK11BI GGWCC 1 cut(s) 1043
XapI RAATTY 4 cut(s) 16, 203, 583, 986
XceI RCATGY 1 cut(s) 595
XmiI GTMKAC 2 cut(s) 291, 930
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.