MD04G1243700.v1.1

trigalactosyldiacylglycerol 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014402
Physical Location & Seq
Reverse (-)
31994068 .. 31997745
3678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1243700.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGTTTCTGTGTCGAATTCAGTGTTGTTTCCTCTGGCGACATCTAGCGGCTTCACATCCGATGGTTTTTCTCGGTCCGTTAGATTTGCAAGGACGATCAATTGTAGTAGTGACAAGCAAAAGGGAGAGCAAAGAAACGTTTTGTGTGCTTGTATGGAGCCTCCCCCCAAACTGCGGAGCGATGAATCCTTTGCAGCTAAATTCGAGGATCCATTTAAATCAGAGGATTTAAGTACAGTTTGGGAACCCGAGGATGACTCTGATGTTCTAATCGAGTGTAGAGACGTCTACAAATCGTTTGGCGAGAAGCATATATTGAGAGGTGTGAGCTTCAAGATTAGGCATGGCGAAGCTGTTGGAATAATTGGGCCTTCTGGCACGGGAAAATCTACAGTTCTGAAGATTATGGCAGGGCTTCTTGCTCCTGACAAGGGAGAGGTGTACATTCGAGGTCGAAAGAGAATTGGTTTGATCAGCGATGATGAGATATCTGGTCTTCGGATTGGATTGGTGTTTCAGAGCGCTGCACTATTTGATTCTTTGACTGTTCGTGATAATGTTGGTTTCCTTCTGTATGAAAATTCGAGCATGCCCGAGGATCAAATTTCAAAGCTTGTGACTGAGAGCTTGGCCGCAGTCGGGTTAAAGGGAGTTGAGGATCGATTACCTTCGGAGCTATCTGGTGGAATGAAGAAACGAGTTGCTCTGGCTCGATCCATAATTTATGATACCACAAAGGAGGCGATAGAGCCAGAGGTGCTCTTATATGATGAGCCAACAGCTGGACTTGATCCTATTGCTTCTACTGTGGTAGAAGACCTCATCCGCTCTGTACATACAAAAGGTCGGGATGCAATCAAGAAACCTGGGCAGATCGCATCTTACGTTGTTGTTACTCACCAACATAGTACCATCAGACGAGCTGTTGACAGGTTGTTGTTTCTCTACGAGGGCAAGATAGTATGGCAAGGAATGACTCATGAATTCACAACCTCAACAAATCCAATCGTTCAACAGTTTGCTTCCGGGAGCTTGGACGGCCCGATTAAATATTAG

Protein Analysis

352

Amino Acids

38.49

Weight (kDa)

5.96

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 106 - 261 2e-29 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65410
fragaria_vesca FvH4_6g00670 FvH4_6g00670
malus_domestica MD04G1243700.v1.1 MD12G1259300.v1.1
prunus_persica Prupe.6G359900_v2.0.a1
pyrus_communis pycom04g21440 pycom12g23770
rosa_chinensis RchiOBHm_Chr3g0448111
rosa_laevigata RLG00000025946
rosa_multiflora Rmu_sc0005969.1_g000027
rosa_roxburghii Rroxscaffold_6G00426240
rosa_rugosa Rorug02G0608200
rosa_samantha Rh3AG007700 Rh3CG007400 Rh3DG007900
rosa_wichuraiana Rw3G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
AccB7I CCANNNNNTGG 1 cut(s) 782
AccBSI CCGCTC 1 cut(s) 828
AccI GTMKAC 1 cut(s) 288
AciI CCGC 4 cut(s) 48, 175, 633, 826
AclI AACGTT 1 cut(s) 138
AclWI GGATC 6 cut(s) 203, 216, 606, 666, 708, 785
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 5 cut(s) 16, 200, 580, 603, 983
AcuI CTGAAG 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 4 cut(s) 235, 443, 834, 910
AfeI AGCGCT 1 cut(s) 523
AfiI CCNNNNNNNGG 4 cut(s) 174, 431, 639, 782
AgsI TTSAA 3 cut(s) 334, 609, 1013
AjnI CCWGG 1 cut(s) 865
AluBI AGCT 9 cut(s) 197, 330, 353, 613, 627, 676, 782, 923, 1032
AluI AGCT 9 cut(s) 197, 330, 353, 613, 627, 676, 782, 923, 1032
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 6 cut(s) 203, 216, 606, 666, 708, 785
Ama87I CYCGRG 2 cut(s) 248, 593
Aor51HI AGCGCT 1 cut(s) 523
AoxI GGCC 3 cut(s) 368, 630, 1039
ApeKI GCWGC 2 cut(s) 194, 524
ApoI RAATTY 5 cut(s) 16, 200, 580, 603, 983
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 3 cut(s) 75, 368, 1040
AsuC2I CCSGG 1 cut(s) 1027
AsuHPI GGTGA 1 cut(s) 890
AvaI CYCGRG 2 cut(s) 248, 593
AvaII GGWCC 1 cut(s) 75
BamHI GGATCC 1 cut(s) 208
BbsI GAAGAC 2 cut(s) 488, 822
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 2 cut(s) 206, 511
BccI CCATC 2 cut(s) 56, 920
BceAI ACGGC 1 cut(s) 1054
BciT130I CCWGG 1 cut(s) 867
BclI TGATCA 1 cut(s) 471
BcnI CCSGG 1 cut(s) 1027
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 1 cut(s) 45
BfmI CTRYAG 1 cut(s) 390
BfoI RGCGCY 1 cut(s) 525
BisI GCNGC 4 cut(s) 49, 195, 525, 633
BlsI GCNGC 4 cut(s) 50, 196, 526, 634
Bme1390I CCNGG 2 cut(s) 867, 1027
Bme18I GGWCC 1 cut(s) 75
BmeT110I CYCGRG 2 cut(s) 248, 593
BmgT120I GGNCC 3 cut(s) 75, 368, 1040
BmiI GGNNCC 3 cut(s) 159, 210, 246
BmrFI CCNGG 2 cut(s) 867, 1027
BmsI GCATC 2 cut(s) 841, 887
BpiI GAAGAC 2 cut(s) 488, 822
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpuMI CCSGG 1 cut(s) 1027
Bsa29I ATCGAT 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 249, 594, 866
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 431, 639, 782
BseBI CCWGG 1 cut(s) 867
BseCI ATCGAT 1 cut(s) 661
BseDI CCNNGG 3 cut(s) 249, 594, 866
BseGI GGATG 4 cut(s) 56, 259, 822, 856
BseLI CCNNNNNNNGG 4 cut(s) 174, 431, 639, 782
BseMII CTCAG 1 cut(s) 612
BseXI GCAGC 2 cut(s) 206, 511
BsgI GTGCAG 1 cut(s) 510
BshFI GGCC 3 cut(s) 370, 632, 1041
BshVI ATCGAT 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 2 cut(s) 248, 593
BsiSI CCGG 1 cut(s) 1026
BslI CCNNNNNNNGG 4 cut(s) 174, 431, 639, 782
BsmAI GTCTC 1 cut(s) 276
BsmBI CGTCTC 1 cut(s) 276
BsnI GGCC 3 cut(s) 370, 632, 1041
BsoBI CYCGRG 2 cut(s) 248, 593
Bsp1286I GDGCHC 1 cut(s) 762
Bsp1407I TGTACA 2 cut(s) 441, 832
Bsp143I GATC 8 cut(s) 96, 208, 471, 598, 658, 713, 790, 873
BspACI CCGC 4 cut(s) 48, 175, 633, 826
BspANI GGCC 3 cut(s) 370, 632, 1041
BspCNI CTCAG 1 cut(s) 613
BspDI ATCGAT 1 cut(s) 661
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 3 cut(s) 159, 210, 246
BspPI GGATC 6 cut(s) 203, 216, 606, 666, 708, 785
BsrBI CCGCTC 1 cut(s) 828
BsrGI TGTACA 2 cut(s) 441, 832
BssECI CCNNGG 3 cut(s) 249, 594, 866
BssMI GATC 8 cut(s) 96, 208, 471, 598, 658, 713, 790, 873
BssNI GRCGYC 1 cut(s) 285
Bst2UI CCWGG 1 cut(s) 867
Bst4CI ACNGT 5 cut(s) 238, 394, 547, 808, 1017
BstACI GRCGYC 1 cut(s) 285
BstAUI TGTACA 2 cut(s) 441, 832
BstC8I GCNNGC 1 cut(s) 590
BstDEI CTNAG 1 cut(s) 621
BstF5I GGATG 4 cut(s) 56, 259, 822, 856
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 8 cut(s) 99, 211, 474, 601, 661, 716, 793, 876
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 8 cut(s) 96, 208, 471, 598, 658, 713, 790, 873
BstMWI GCNNNNNNNGC 2 cut(s) 757, 1038
BstNI CCWGG 1 cut(s) 867
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 2 cut(s) 865, 1025
BstSFI CTRYAG 1 cut(s) 390
BstV1I GCAGC 2 cut(s) 206, 511
BstV2I GAAGAC 2 cut(s) 488, 822
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
Bsu15I ATCGAT 1 cut(s) 661
BsuRI GGCC 3 cut(s) 370, 632, 1041
BsuTUI ATCGAT 1 cut(s) 661
BtgZI GCGATG 2 cut(s) 195, 492
BtsCI GGATG 4 cut(s) 56, 259, 822, 856
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 590
CciI TCATGA 1 cut(s) 979
CfoI GCGC 1 cut(s) 524
Cfr13I GGNCC 3 cut(s) 75, 368, 1040
ClaI ATCGAT 1 cut(s) 661
CpoI CGGWCCG 1 cut(s) 75
Csp6I GTAC 4 cut(s) 234, 442, 833, 909
CspI CGGWCCG 1 cut(s) 75
CviAII CATG 3 cut(s) 344, 589, 980
CviQI GTAC 4 cut(s) 234, 442, 833, 909
DdeI CTNAG 1 cut(s) 621
DpnI GATC 8 cut(s) 98, 210, 473, 600, 660, 715, 792, 875
DpnII GATC 8 cut(s) 96, 208, 471, 598, 658, 713, 790, 873
DraI TTTAAA 1 cut(s) 217
EaeI YGGCCR 1 cut(s) 630
Eco32I GATATC 1 cut(s) 489
Eco47I GGWCC 1 cut(s) 75
Eco47III AGCGCT 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 419
Eco88I CYCGRG 2 cut(s) 248, 593
EcoRI GAATTC 2 cut(s) 16, 983
EcoRII CCWGG 1 cut(s) 865
EcoRV GATATC 1 cut(s) 489
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 3 cut(s) 347, 592, 983
FatI CATG 3 cut(s) 343, 588, 979
FbaI TGATCA 1 cut(s) 471
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 4 cut(s) 49, 195, 525, 633
FokI GGATG 4 cut(s) 43, 266, 809, 863
Fsp4HI GCNGC 4 cut(s) 49, 195, 525, 633
FspBI CTAG 1 cut(s) 45
GlaI GCGC 1 cut(s) 523
GluI GCNGC 4 cut(s) 49, 195, 525, 633
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 3 cut(s) 370, 632, 1041
HapII CCGG 1 cut(s) 1026
HhaI GCGC 1 cut(s) 524
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 3 cut(s) 347, 592, 983
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 4 cut(s) 185, 257, 536, 976
HpaII CCGG 1 cut(s) 1026
HphI GGTGA 1 cut(s) 890
Hpy166II GTNNAC 3 cut(s) 289, 442, 928
Hpy188I TCNGA 8 cut(s) 61, 223, 262, 399, 501, 519, 673, 917
Hpy188III TCNNGA 6 cut(s) 334, 425, 551, 848, 859, 980
Hpy8I GTNNAC 3 cut(s) 289, 442, 928
HpyAV CCTTC 3 cut(s) 381, 578, 678
HpyCH4III ACNGT 5 cut(s) 238, 394, 547, 808, 1017
HpyCH4IV ACGT 3 cut(s) 138, 285, 885
HpyCH4V TGCA 4 cut(s) 89, 194, 527, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 757, 1038
HpyF3I CTNAG 1 cut(s) 621
HpySE526I ACGT 3 cut(s) 138, 285, 885
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 3 cut(s) 347, 592, 983
HspAI GCGC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 471
Kzo9I GATC 8 cut(s) 96, 208, 471, 598, 658, 713, 790, 873
LmnI GCTCC 5 cut(s) 157, 177, 427, 673, 1029
Lsp1109I GCAGC 2 cut(s) 206, 511
LweI GCATC 2 cut(s) 841, 887
MaeI CTAG 1 cut(s) 45
MaeII ACGT 3 cut(s) 138, 285, 885
MaeIII GTNAC 3 cut(s) 110, 616, 892
MalI GATC 8 cut(s) 98, 210, 473, 600, 660, 715, 792, 875
MbiI CCGCTC 1 cut(s) 828
MboI GATC 8 cut(s) 96, 208, 471, 598, 658, 713, 790, 873
MboII GAAGA 4 cut(s) 412, 488, 703, 827
MfeI CAATTG 1 cut(s) 100
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 762
MluCI AATT 9 cut(s) 16, 100, 200, 363, 462, 580, 603, 720, 983
MlyI GAGTC 2 cut(s) 251, 970
MmeI TCCRAC 1 cut(s) 337
MseI TTAA 4 cut(s) 216, 230, 644, 1047
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 1 cut(s) 1026
MspR9I CCNGG 2 cut(s) 867, 1027
MunI CAATTG 1 cut(s) 100
MvaI CCWGG 1 cut(s) 867
MwoI GCNNNNNNNGC 2 cut(s) 757, 1038
NciI CCSGG 1 cut(s) 1027
NdeII GATC 8 cut(s) 96, 208, 471, 598, 658, 713, 790, 873
NlaIII CATG 3 cut(s) 347, 592, 983
NlaIV GGNNCC 3 cut(s) 159, 210, 246
NmuCI GTSAC 2 cut(s) 110, 616
NspI RCATGY 1 cut(s) 592
PaeI GCATGC 1 cut(s) 592
PagI TCATGA 1 cut(s) 979
PcsI WCGNNNNNNNCGW 1 cut(s) 882
PfeI GAWTC 2 cut(s) 185, 536
PflMI CCANNNNNTGG 1 cut(s) 782
PfoI TCCNGGA 1 cut(s) 1025
PkrI GCNGC 4 cut(s) 50, 196, 526, 634
PleI GAGTC 2 cut(s) 251, 970
PpsI GAGTC 2 cut(s) 251, 970
Psp1406I AACGTT 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 865
PspGI CCWGG 1 cut(s) 865
PspN4I GGNNCC 3 cut(s) 159, 210, 246
PspPI GGNCC 3 cut(s) 75, 368, 1040
PsuI RGATCY 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 4 cut(s) 235, 443, 834, 910
RsaNI GTAC 4 cut(s) 234, 442, 833, 909
Rsr2I CGGWCCG 1 cut(s) 75
RsrII CGGWCCG 1 cut(s) 75
SaqAI TTAA 4 cut(s) 216, 230, 644, 1047
SatI GCNGC 4 cut(s) 49, 195, 525, 633
Sau3AI GATC 8 cut(s) 96, 208, 471, 598, 658, 713, 790, 873
Sau96I GGNCC 3 cut(s) 75, 368, 1040
SchI GAGTC 2 cut(s) 251, 970
ScrFI CCNGG 2 cut(s) 867, 1027
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 2 cut(s) 841, 887
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 75
SmiI ATTTAAAT 1 cut(s) 217
SphI GCATGC 1 cut(s) 592
Sse9I AATT 9 cut(s) 16, 100, 200, 363, 462, 580, 603, 720, 983
SsiI CCGC 4 cut(s) 48, 175, 633, 826
SspI AATATT 1 cut(s) 1052
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 2 cut(s) 865, 1025
SwaI ATTTAAAT 1 cut(s) 217
TaaI ACNGT 5 cut(s) 238, 394, 547, 808, 1017
TaiI ACGT 3 cut(s) 141, 288, 888
TaqI TCGA 8 cut(s) 14, 204, 273, 448, 454, 584, 661, 712
TaqII GACCGA 1 cut(s) 63
TasI AATT 9 cut(s) 16, 100, 200, 363, 462, 580, 603, 720, 983
TatI WGTACW 3 cut(s) 233, 441, 832
TauI GCSGC 2 cut(s) 51, 635
TfiI GAWTC 2 cut(s) 185, 536
Tru1I TTAA 4 cut(s) 216, 230, 644, 1047
Tru9I TTAA 4 cut(s) 216, 230, 644, 1047
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 2 cut(s) 110, 616
TseI GCWGC 2 cut(s) 194, 524
Tsp45I GTSAC 2 cut(s) 110, 616
TspDTI ATGAA 4 cut(s) 198, 591, 704, 996
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 27
Van91I CCANNNNNTGG 1 cut(s) 782
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 5 cut(s) 16, 200, 580, 603, 983
XceI RCATGY 1 cut(s) 592
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 1 cut(s) 45
ZraI GACGTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.