pycom04g21440

trigalactosyldiacylglycerol 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014402
Physical Location & Seq
Reverse (-)
22604434 .. 22607572
3139 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21440.2

Sequence Viewer

Length: 1056 bp
ATGGTTTCTGTGTCGAATTCAGTGTTGTTTCCTCTGGCGACATCCAACGGCTTCACATCCGATGGTTTTTCTCGGTCCGTTAGATTTGCAAGGACGATCAATTGTAGTAGTGACAAGCAAAAGGGAGAGCAAAGAAACGTTTTGTGTGCTTGTATGGAGCCTCCCCCCAAACTGCGGAGCGATGAATCCTTTGCAGCTAAATTCGAGGATTCATTTAAATCAGAGGATTTGAGTACAGTTTGGGAGCCCAAGGATGACTCTGATGTTCTAATCGAGTGTAGAGACGTCTACAAATCGTTTGGTGAGAAGCATATATTGAGAGGTGTGAGCTTCAAGATTAGGCATGGCGAAGCTGTTGGAATAATTGGGCCTTCTGGCACGGGAAAATCTACAGTTCTGAAGATTATGGCAGGGCTTCTCACTCCTGACAAGGGAGAGGTGTACATTCGAGGTCGAAAGAGAGTTGGTTTGATCAGCGATGATGAGATGTCTGGTCTTCGGATTGGATTGGTGTTTCAGAGCGCTGCACTATTTGATTCTTTGACTGTTCGTGAAAATGTTGGTTTCCTTCTGTATGAAAATTCGAGCATGCCCGAGGATCAAATTTCAAAGCTTGTGACTGAGAGCTTGGCCGCAGTCGGGTTAAAGGGAGTTGAGGATCGATTACCTTCGGAGCTATCTGGTGGAATGAAGAAACGAGTTGCTCTGGCTCGATCCATAATTTATGATACCACAAAGGAGGCGATAGAGCCAGAGGTGCTCTTATATGATGAGCCAACAGCTGGACTTGATCCTATTGCTTCTACAGTGGTAGAAGACCTCATCCGCTCTGTCCATACAAAAGGTCGCGATGCAATCAAGAAACCTGGGCAGATCGCATCTTATGTTGTTGTTACTCACCAACATAGTACCATCAGACGAGCTGTTGACAGGCTGTTGTTTCTCTACGAGGGCAAGATAGTGTGGCAAGGAATGACTCATGAATTCACAACCTCAACAAATCCAATCGTTCAACAGTTTGCTTCCGGGAGCTTGGACGGCCCGATTAAATATTAG

Protein Analysis

352

Amino Acids

38.56

Weight (kDa)

6.35

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65410
fragaria_vesca FvH4_6g00670 FvH4_6g00670
malus_domestica MD04G1243700.v1.1 MD12G1259300.v1.1
prunus_persica Prupe.6G359900_v2.0.a1
pyrus_communis pycom04g21440 pycom12g23770
rosa_chinensis RchiOBHm_Chr3g0448111
rosa_laevigata RLG00000025946
rosa_multiflora Rmu_sc0005969.1_g000027
rosa_roxburghii Rroxscaffold_6G00426240
rosa_rugosa Rorug02G0608200
rosa_samantha Rh3AG007700 Rh3CG007400 Rh3DG007900
rosa_wichuraiana Rw3G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
AccB7I CCANNNNNTGG 1 cut(s) 782
AccBSI CCGCTC 1 cut(s) 828
AccI GTMKAC 1 cut(s) 288
AccII CGCG 1 cut(s) 849
AciI CCGC 3 cut(s) 175, 633, 826
AclI AACGTT 1 cut(s) 138
AclWI GGATC 4 cut(s) 606, 666, 708, 785
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 5 cut(s) 16, 200, 580, 603, 983
AcuI CTGAAG 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 3 cut(s) 235, 443, 910
AfeI AGCGCT 1 cut(s) 523
AfiI CCNNNNNNNGG 4 cut(s) 174, 431, 639, 782
AgsI TTSAA 3 cut(s) 334, 609, 1013
AjnI CCWGG 1 cut(s) 865
AluBI AGCT 9 cut(s) 197, 330, 353, 613, 627, 676, 782, 923, 1032
AluI AGCT 9 cut(s) 197, 330, 353, 613, 627, 676, 782, 923, 1032
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 4 cut(s) 606, 666, 708, 785
Ama87I CYCGRG 1 cut(s) 593
Aor51HI AGCGCT 1 cut(s) 523
AoxI GGCC 3 cut(s) 368, 630, 1039
ApeKI GCWGC 2 cut(s) 194, 524
ApoI RAATTY 5 cut(s) 16, 200, 580, 603, 983
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 3 cut(s) 75, 368, 1040
AsuC2I CCSGG 1 cut(s) 1027
AsuHPI GGTGA 2 cut(s) 314, 890
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 1 cut(s) 75
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 2 cut(s) 488, 822
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 2 cut(s) 206, 511
BccI CCATC 2 cut(s) 56, 920
BceAI ACGGC 2 cut(s) 64, 1054
BciT130I CCWGG 1 cut(s) 867
BclI TGATCA 1 cut(s) 471
BcnI CCSGG 1 cut(s) 1027
BcoDI GTCTC 1 cut(s) 276
BfmI CTRYAG 2 cut(s) 390, 804
BfoI RGCGCY 1 cut(s) 525
BisI GCNGC 3 cut(s) 195, 525, 633
BlsI GCNGC 3 cut(s) 196, 526, 634
Bme1390I CCNGG 2 cut(s) 867, 1027
Bme18I GGWCC 1 cut(s) 75
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 3 cut(s) 75, 368, 1040
BmiI GGNNCC 2 cut(s) 159, 246
BmrFI CCNGG 2 cut(s) 867, 1027
BmsI GCATC 2 cut(s) 841, 887
BpiI GAAGAC 2 cut(s) 488, 822
BpuMI CCSGG 1 cut(s) 1027
Bsa29I ATCGAT 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 249, 594, 866
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 431, 639, 782
BseBI CCWGG 1 cut(s) 867
BseCI ATCGAT 1 cut(s) 661
BseDI CCNNGG 3 cut(s) 249, 594, 866
BseGI GGATG 4 cut(s) 41, 56, 259, 822
BseLI CCNNNNNNNGG 4 cut(s) 174, 431, 639, 782
BseMII CTCAG 1 cut(s) 612
BseXI GCAGC 2 cut(s) 206, 511
BsgI GTGCAG 1 cut(s) 510
Bsh1236I CGCG 1 cut(s) 849
BshFI GGCC 3 cut(s) 370, 632, 1041
BshVI ATCGAT 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 1 cut(s) 1026
BslI CCNNNNNNNGG 4 cut(s) 174, 431, 639, 782
BsmAI GTCTC 1 cut(s) 276
BsmBI CGTCTC 1 cut(s) 276
BsnI GGCC 3 cut(s) 370, 632, 1041
BsoBI CYCGRG 1 cut(s) 593
Bsp1286I GDGCHC 2 cut(s) 249, 762
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 7 cut(s) 96, 471, 598, 658, 713, 790, 873
Bsp68I TCGCGA 1 cut(s) 849
BspACI CCGC 3 cut(s) 175, 633, 826
BspANI GGCC 3 cut(s) 370, 632, 1041
BspCNI CTCAG 1 cut(s) 613
BspDI ATCGAT 1 cut(s) 661
BspFNI CGCG 1 cut(s) 849
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 2 cut(s) 159, 246
BspPI GGATC 4 cut(s) 606, 666, 708, 785
BsrBI CCGCTC 1 cut(s) 828
BsrGI TGTACA 1 cut(s) 441
BssECI CCNNGG 3 cut(s) 249, 594, 866
BssMI GATC 7 cut(s) 96, 471, 598, 658, 713, 790, 873
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 867
Bst4CI ACNGT 5 cut(s) 238, 394, 547, 808, 1017
BstACI GRCGYC 1 cut(s) 285
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 590
BstDEI CTNAG 1 cut(s) 621
BstF5I GGATG 4 cut(s) 41, 56, 259, 822
BstFNI CGCG 1 cut(s) 849
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 7 cut(s) 99, 474, 601, 661, 716, 793, 876
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 7 cut(s) 96, 471, 598, 658, 713, 790, 873
BstMWI GCNNNNNNNGC 2 cut(s) 757, 1038
BstNI CCWGG 1 cut(s) 867
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 2 cut(s) 865, 1025
BstSFI CTRYAG 2 cut(s) 390, 804
BstUI CGCG 1 cut(s) 849
BstV1I GCAGC 2 cut(s) 206, 511
BstV2I GAAGAC 2 cut(s) 488, 822
Bsu15I ATCGAT 1 cut(s) 661
BsuRI GGCC 3 cut(s) 370, 632, 1041
BsuTUI ATCGAT 1 cut(s) 661
BtgZI GCGATG 3 cut(s) 195, 492, 864
BtsCI GGATG 4 cut(s) 41, 56, 259, 822
BtsIMutI CAGTG 2 cut(s) 27, 813
BtuMI TCGCGA 1 cut(s) 849
Cac8I GCNNGC 1 cut(s) 590
CciI TCATGA 1 cut(s) 979
CfoI GCGC 1 cut(s) 524
Cfr13I GGNCC 3 cut(s) 75, 368, 1040
ClaI ATCGAT 1 cut(s) 661
CpoI CGGWCCG 1 cut(s) 75
Csp6I GTAC 3 cut(s) 234, 442, 909
CspI CGGWCCG 1 cut(s) 75
CviAII CATG 3 cut(s) 344, 589, 980
CviQI GTAC 3 cut(s) 234, 442, 909
DdeI CTNAG 1 cut(s) 621
DpnI GATC 7 cut(s) 98, 473, 600, 660, 715, 792, 875
DpnII GATC 7 cut(s) 96, 471, 598, 658, 713, 790, 873
DraI TTTAAA 1 cut(s) 217
EaeI YGGCCR 1 cut(s) 630
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 75
Eco47III AGCGCT 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 419
Eco88I CYCGRG 1 cut(s) 593
EcoRI GAATTC 2 cut(s) 16, 983
EcoRII CCWGG 1 cut(s) 865
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 3 cut(s) 347, 592, 983
FatI CATG 3 cut(s) 343, 588, 979
FbaI TGATCA 1 cut(s) 471
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 195, 525, 633
FokI GGATG 4 cut(s) 28, 43, 266, 809
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 3 cut(s) 195, 525, 633
GlaI GCGC 1 cut(s) 523
GluI GCNGC 3 cut(s) 195, 525, 633
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 3 cut(s) 370, 632, 1041
HapII CCGG 1 cut(s) 1026
HhaI GCGC 1 cut(s) 524
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 3 cut(s) 347, 592, 983
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 5 cut(s) 185, 209, 257, 536, 976
HpaII CCGG 1 cut(s) 1026
HphI GGTGA 2 cut(s) 314, 890
Hpy166II GTNNAC 3 cut(s) 289, 442, 928
Hpy188I TCNGA 8 cut(s) 61, 223, 262, 399, 501, 519, 673, 917
Hpy188III TCNNGA 6 cut(s) 334, 425, 551, 848, 859, 980
Hpy8I GTNNAC 3 cut(s) 289, 442, 928
HpyAV CCTTC 3 cut(s) 381, 578, 678
HpyCH4III ACNGT 5 cut(s) 238, 394, 547, 808, 1017
HpyCH4IV ACGT 2 cut(s) 138, 285
HpyCH4V TGCA 4 cut(s) 89, 194, 527, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 757, 1038
HpyF3I CTNAG 1 cut(s) 621
HpySE526I ACGT 2 cut(s) 138, 285
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 3 cut(s) 347, 592, 983
HspAI GCGC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 471
Kzo9I GATC 7 cut(s) 96, 471, 598, 658, 713, 790, 873
LmnI GCTCC 5 cut(s) 157, 177, 244, 673, 1029
Lsp1109I GCAGC 2 cut(s) 206, 511
LweI GCATC 2 cut(s) 841, 887
MaeII ACGT 2 cut(s) 138, 285
MaeIII GTNAC 3 cut(s) 110, 616, 892
MalI GATC 7 cut(s) 98, 473, 600, 660, 715, 792, 875
MbiI CCGCTC 1 cut(s) 828
MboI GATC 7 cut(s) 96, 471, 598, 658, 713, 790, 873
MboII GAAGA 4 cut(s) 412, 488, 703, 827
MfeI CAATTG 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 249, 762
MluCI AATT 8 cut(s) 16, 100, 200, 363, 580, 603, 720, 983
MlyI GAGTC 2 cut(s) 251, 970
MmeI TCCRAC 2 cut(s) 69, 337
MseI TTAA 3 cut(s) 216, 644, 1047
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 1 cut(s) 1026
MspR9I CCNGG 2 cut(s) 867, 1027
MunI CAATTG 1 cut(s) 100
MvaI CCWGG 1 cut(s) 867
MvnI CGCG 1 cut(s) 849
MwoI GCNNNNNNNGC 2 cut(s) 757, 1038
NciI CCSGG 1 cut(s) 1027
NdeII GATC 7 cut(s) 96, 471, 598, 658, 713, 790, 873
NlaIII CATG 3 cut(s) 347, 592, 983
NlaIV GGNNCC 2 cut(s) 159, 246
NmuCI GTSAC 2 cut(s) 110, 616
NruI TCGCGA 1 cut(s) 849
NspI RCATGY 1 cut(s) 592
PaeI GCATGC 1 cut(s) 592
PagI TCATGA 1 cut(s) 979
PfeI GAWTC 3 cut(s) 185, 209, 536
PflMI CCANNNNNTGG 1 cut(s) 782
PfoI TCCNGGA 1 cut(s) 1025
PkrI GCNGC 3 cut(s) 196, 526, 634
PleI GAGTC 2 cut(s) 251, 970
PpsI GAGTC 2 cut(s) 251, 970
Psp1406I AACGTT 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 865
PspGI CCWGG 1 cut(s) 865
PspN4I GGNNCC 2 cut(s) 159, 246
PspPI GGNCC 3 cut(s) 75, 368, 1040
PvuII CAGCTG 1 cut(s) 782
RruI TCGCGA 1 cut(s) 849
RsaI GTAC 3 cut(s) 235, 443, 910
RsaNI GTAC 3 cut(s) 234, 442, 909
Rsr2I CGGWCCG 1 cut(s) 75
RsrII CGGWCCG 1 cut(s) 75
SaqAI TTAA 3 cut(s) 216, 644, 1047
SatI GCNGC 3 cut(s) 195, 525, 633
Sau3AI GATC 7 cut(s) 96, 471, 598, 658, 713, 790, 873
Sau96I GGNCC 3 cut(s) 75, 368, 1040
SchI GAGTC 2 cut(s) 251, 970
ScrFI CCNGG 2 cut(s) 867, 1027
SduI GDGCHC 2 cut(s) 249, 762
SfaNI GCATC 2 cut(s) 841, 887
SfcI CTRYAG 2 cut(s) 390, 804
SinI GGWCC 1 cut(s) 75
SmiI ATTTAAAT 1 cut(s) 217
SphI GCATGC 1 cut(s) 592
Sse9I AATT 8 cut(s) 16, 100, 200, 363, 580, 603, 720, 983
SsiI CCGC 3 cut(s) 175, 633, 826
SspI AATATT 1 cut(s) 1052
StyD4I CCNGG 2 cut(s) 865, 1025
StyI CCWWGG 1 cut(s) 249
SwaI ATTTAAAT 1 cut(s) 217
TaaI ACNGT 5 cut(s) 238, 394, 547, 808, 1017
TaiI ACGT 2 cut(s) 141, 288
TaqI TCGA 8 cut(s) 14, 204, 273, 448, 454, 584, 661, 712
TaqII GACCGA 1 cut(s) 63
TasI AATT 8 cut(s) 16, 100, 200, 363, 580, 603, 720, 983
TatI WGTACW 2 cut(s) 233, 441
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 3 cut(s) 185, 209, 536
Tru1I TTAA 3 cut(s) 216, 644, 1047
Tru9I TTAA 3 cut(s) 216, 644, 1047
TscAI CASTG 2 cut(s) 27, 813
TseFI GTSAC 2 cut(s) 110, 616
TseI GCWGC 2 cut(s) 194, 524
Tsp45I GTSAC 2 cut(s) 110, 616
TspDTI ATGAA 5 cut(s) 198, 201, 591, 704, 996
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 2 cut(s) 27, 813
Van91I CCANNNNNTGG 1 cut(s) 782
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 5 cut(s) 16, 200, 580, 603, 983
XceI RCATGY 1 cut(s) 592
XmiI GTMKAC 1 cut(s) 288
ZraI GACGTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.