Rh3CG007400

trigalactosyldiacylglycerol 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0014402
Physical Location & Seq
Forward (+)
521411 .. 525497
4087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG007400.1

Sequence Viewer

Length: 1044 bp
ATGGGAAGAAGCATGGTTTCTCTGTCGAATTCAGTGTTGTTTCCTGTGACTACATCCAATGGCATTTCTCGGCCGGCTAGGTTTCCAAAGACGATCAATTTGTGTAGTTATAAGCAGAAAAGAGAGCCCAGAAACATTGTTTGTGCTTGTATGGCGCCTCCCCAGAACTATGAATCCTATGCAGCAAAATTCGAGGATTCATTGAAATCAGAGGATTTATCCACAGTAAGAGAACCCGAGGATGACTCTGATGTTCTGATTGAGTGCAGAGACGTCCATAAGTCATTTGGTGAGAAGGATATATTGAGAGGTGTGAGCTTCAAGATTAGACATGGCGAAGCAGTTGGAATAATTGGTCCTTCTGGCACCGGGAAATCTACAGTTTTAAAGATTATGGCTGGTCTTCTTGCGCCTGACAAGGGAGAGGTATACATTCGAGGTCGAAAGAGGGATGGTTTGATCAGCGATGAGGAGTTATCTGGTCTTCGAATTGGATTGGTGTTTCAGAGTGCTGCACTTTTTGATTCGTTGACTGTTCGTGAAAATGTTGGTTTCCTTCTGTATGAAAATTCAAGCATGCCCGAGGAACAAATTTCAAAGCTCGTGACTGATAGCTTGGCTGCTGTTGGGTTAAAGGGAGTTGAGGATCGATTACCATCGGAGCTGTCTGGTGGAATGAAGAAAAGAGTTGCATTGGCTCGATCTATAATTTGTGATACCACAAAGGAAGCCATAGAGCCAGAGGTGCTCTTATATGATGAACCAACAGCTGGACTTGATCCGATTGCATCTACTGTTGTAGAAGACCTCATCCGCTCTGTCCATACAGAAGAGCAGGCAGCGTCAGAGAAGTCTGGGCAGATTGCGTCTTACGTTGTTGTAACTCACCAACATAGCACCATCAGACGAGCTGTTGACAGGTTATTGTTTCTGTATGAGGGCAAGATAGTCTGGCAAGGAATGACTGATGAATTCACAACCTCAACAAATCCAATCGTTCAACAGTTTGCATCTGGGAGCTTGGATGGCCCAATTAAATATTAG

Protein Analysis

347

Amino Acids

38.02

Weight (kDa)

5.47

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 102 - 257 8.4e-31 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65410
fragaria_vesca FvH4_6g00670 FvH4_6g00670
malus_domestica MD04G1243700.v1.1 MD12G1259300.v1.1
prunus_persica Prupe.6G359900_v2.0.a1
pyrus_communis pycom04g21440 pycom12g23770
rosa_chinensis RchiOBHm_Chr3g0448111
rosa_laevigata RLG00000025946
rosa_multiflora Rmu_sc0005969.1_g000027
rosa_roxburghii Rroxscaffold_6G00426240
rosa_rugosa Rorug02G0608200
rosa_samantha Rh3AG007700 Rh3CG007400 Rh3DG007900
rosa_wichuraiana Rw3G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 111
AatII GACGTC 1 cut(s) 276
AccB1I GGYRCC 2 cut(s) 154, 365
AccB7I CCANNNNNTGG 1 cut(s) 770
AccBSI CCGCTC 1 cut(s) 816
AccI GTMKAC 1 cut(s) 429
AciI CCGC 1 cut(s) 814
AclWI GGATC 2 cut(s) 654, 773
AcoI YGGCCR 1 cut(s) 71
AcsI RAATTY 5 cut(s) 28, 188, 568, 591, 971
AcyI GRCGYC 2 cut(s) 155, 273
AfiI CCNNNNNNNGG 2 cut(s) 419, 770
AgsI TTSAA 5 cut(s) 205, 322, 573, 597, 1001
AloI GAACNNNNNNTCC 2 cut(s) 158, 190
AluBI AGCT 7 cut(s) 318, 601, 615, 664, 770, 911, 1020
AluI AGCT 7 cut(s) 318, 601, 615, 664, 770, 911, 1020
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 2 cut(s) 654, 773
Ama87I CYCGRG 2 cut(s) 236, 581
AoxI GGCC 2 cut(s) 71, 1027
ApeKI GCWGC 4 cut(s) 182, 512, 620, 839
ApoI RAATTY 5 cut(s) 28, 188, 568, 591, 971
ArsI GACNNNNNNTTYG 2 cut(s) 82, 114
AspLEI GCGC 2 cut(s) 157, 412
AspS9I GGNCC 2 cut(s) 356, 1028
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 2 cut(s) 302, 878
AsuII TTCGAA 1 cut(s) 487
AvaI CYCGRG 2 cut(s) 236, 581
AvaII GGWCC 1 cut(s) 356
BanI GGYRCC 2 cut(s) 154, 365
BanII GRGCYC 1 cut(s) 129
BauI CACGAG 1 cut(s) 602
BbsI GAAGAC 3 cut(s) 395, 476, 810
Bbv12I GWGCWC 1 cut(s) 750
BbvI GCAGC 4 cut(s) 194, 499, 607, 851
BccI CCATC 4 cut(s) 446, 664, 908, 1019
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 264
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 4 cut(s) 183, 513, 621, 840
BlsI GCNGC 4 cut(s) 184, 514, 622, 841
Bme1390I CCNGG 1 cut(s) 370
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 2 cut(s) 236, 581
BmgT120I GGNCC 2 cut(s) 356, 1028
BmiI GGNNCC 2 cut(s) 156, 367
BmrFI CCNGG 1 cut(s) 370
BmsI GCATC 2 cut(s) 797, 1019
BpiI GAAGAC 3 cut(s) 395, 476, 810
BplI GAGNNNNNCTC 2 cut(s) 230, 262
Bpu14I TTCGAA 1 cut(s) 487
BpuMI CCSGG 1 cut(s) 370
Bsa29I ATCGAT 1 cut(s) 649
BsaBI GATNNNNATC 1 cut(s) 655
BsaHI GRCGYC 2 cut(s) 155, 273
BsaJI CCNNGG 2 cut(s) 237, 582
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 770
Bse118I RCCGGY 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 655
BseCI ATCGAT 1 cut(s) 649
BseDI CCNNGG 2 cut(s) 237, 582
BseGI GGATG 5 cut(s) 53, 247, 457, 810, 1030
BseJI GATNNNNATC 1 cut(s) 655
BseLI CCNNNNNNNGG 2 cut(s) 419, 770
BseRI GAGGAG 1 cut(s) 485
BseX3I CGGCCG 1 cut(s) 71
BseXI GCAGC 4 cut(s) 194, 499, 607, 851
BsgI GTGCAG 2 cut(s) 286, 498
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 2 cut(s) 73, 1029
BshNI GGYRCC 2 cut(s) 154, 365
BshVI ATCGAT 1 cut(s) 649
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 750
BsiHKCI CYCGRG 2 cut(s) 236, 581
BsiSI CCGG 2 cut(s) 74, 369
BslI CCNNNNNNNGG 2 cut(s) 419, 770
BsmAI GTCTC 1 cut(s) 264
BsmBI CGTCTC 1 cut(s) 264
BsnI GGCC 2 cut(s) 73, 1029
BsoBI CYCGRG 2 cut(s) 236, 581
Bsp119I TTCGAA 1 cut(s) 487
Bsp1286I GDGCHC 2 cut(s) 129, 750
Bsp143I GATC 5 cut(s) 93, 459, 646, 701, 778
BspACI CCGC 1 cut(s) 814
BspANI GGCC 2 cut(s) 73, 1029
BspDI ATCGAT 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 156, 367
BspPI GGATC 2 cut(s) 654, 773
BspQI GCTCTTC 1 cut(s) 825
BspT104I TTCGAA 1 cut(s) 487
BspT107I GGYRCC 2 cut(s) 154, 365
BsrBI CCGCTC 1 cut(s) 816
BsrFI RCCGGY 1 cut(s) 73
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 237, 582
BssMI GATC 5 cut(s) 93, 459, 646, 701, 778
BssNAI GTATAC 1 cut(s) 430
BssNI GRCGYC 2 cut(s) 155, 273
BssSI CACGAG 1 cut(s) 602
Bst1107I GTATAC 1 cut(s) 430
Bst2BI CACGAG 1 cut(s) 602
Bst4CI ACNGT 5 cut(s) 226, 382, 535, 796, 1005
Bst6I CTCTTC 1 cut(s) 825
BstACI GRCGYC 2 cut(s) 155, 273
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 3 cut(s) 75, 578, 837
BstF5I GGATG 5 cut(s) 53, 247, 457, 810, 1030
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 2 cut(s) 157, 412
BstKTI GATC 5 cut(s) 96, 462, 649, 704, 781
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 5 cut(s) 93, 459, 646, 701, 778
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 3 cut(s) 152, 745, 1026
BstNSI RCATGY 1 cut(s) 580
BstSCI CCNGG 1 cut(s) 368
BstSFI CTRYAG 1 cut(s) 378
BstV1I GCAGC 4 cut(s) 194, 499, 607, 851
BstV2I GAAGAC 3 cut(s) 395, 476, 810
BstZ17I GTATAC 1 cut(s) 430
BstZI CGGCCG 1 cut(s) 71
Bsu15I ATCGAT 1 cut(s) 649
BsuRI GGCC 2 cut(s) 73, 1029
BsuTUI ATCGAT 1 cut(s) 649
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 5 cut(s) 53, 247, 457, 810, 1030
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 3 cut(s) 75, 578, 837
CfoI GCGC 2 cut(s) 157, 412
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 2 cut(s) 356, 1028
ClaI ATCGAT 1 cut(s) 649
CseI GACGC 2 cut(s) 831, 855
CviAII CATG 3 cut(s) 13, 332, 577
DinI GGCGCC 1 cut(s) 156
DpnI GATC 5 cut(s) 95, 461, 648, 703, 780
DpnII GATC 5 cut(s) 93, 459, 646, 701, 778
DraI TTTAAA 1 cut(s) 387
EaeI YGGCCR 1 cut(s) 71
EagI CGGCCG 1 cut(s) 71
Eam1104I CTCTTC 1 cut(s) 825
EarI CTCTTC 1 cut(s) 825
EclXI CGGCCG 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 356
Eco52I CGGCCG 1 cut(s) 71
Eco88I CYCGRG 2 cut(s) 236, 581
EcoRI GAATTC 2 cut(s) 28, 971
EcoT38I GRGCYC 1 cut(s) 129
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 3 cut(s) 16, 335, 580
FatI CATG 3 cut(s) 12, 331, 576
FbaI TGATCA 1 cut(s) 459
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 4 cut(s) 183, 513, 621, 840
FokI GGATG 5 cut(s) 40, 254, 464, 797, 1037
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 4 cut(s) 183, 513, 621, 840
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 156, 411
GluI GCNGC 4 cut(s) 183, 513, 621, 840
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 2 cut(s) 73, 1029
HapII CCGG 2 cut(s) 74, 369
HgaI GACGC 2 cut(s) 831, 855
HhaI GCGC 2 cut(s) 157, 412
Hin1I GRCGYC 2 cut(s) 155, 273
Hin1II CATG 3 cut(s) 16, 335, 580
Hin6I GCGC 2 cut(s) 155, 410
HinP1I GCGC 2 cut(s) 155, 410
HincII GTYRAC 2 cut(s) 531, 916
HindII GTYRAC 2 cut(s) 531, 916
HinfI GANTC 4 cut(s) 173, 197, 245, 524
HpaII CCGG 2 cut(s) 74, 369
HphI GGTGA 2 cut(s) 302, 878
Hpy166II GTNNAC 3 cut(s) 430, 531, 916
Hpy188I TCNGA 8 cut(s) 211, 250, 258, 507, 661, 783, 847, 905
Hpy188III TCNNGA 3 cut(s) 322, 539, 604
Hpy8I GTNNAC 3 cut(s) 430, 531, 916
HpyAV CCTTC 3 cut(s) 289, 369, 566
HpyCH4III ACNGT 5 cut(s) 226, 382, 535, 796, 1005
HpyCH4IV ACGT 2 cut(s) 273, 873
HpyCH4V TGCA 6 cut(s) 182, 267, 515, 692, 788, 1010
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 745, 1026
HpySE526I ACGT 2 cut(s) 273, 873
Hsp92I GRCGYC 2 cut(s) 155, 273
Hsp92II CATG 3 cut(s) 16, 335, 580
HspAI GCGC 2 cut(s) 155, 410
KasI GGCGCC 1 cut(s) 154
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 5 cut(s) 93, 459, 646, 701, 778
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 2 cut(s) 661, 1017
Lsp1109I GCAGC 4 cut(s) 194, 499, 607, 851
LweI GCATC 2 cut(s) 797, 1019
MaeI CTAG 1 cut(s) 78
MaeII ACGT 2 cut(s) 273, 873
MaeIII GTNAC 3 cut(s) 46, 604, 880
MalI GATC 5 cut(s) 95, 461, 648, 703, 780
MbiI CCGCTC 1 cut(s) 816
MboI GATC 5 cut(s) 93, 459, 646, 701, 778
MboII GAAGA 6 cut(s) 18, 395, 476, 691, 815, 842
MhlI GDGCHC 2 cut(s) 129, 750
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 325
MroNI GCCGGC 1 cut(s) 73
MseI TTAA 3 cut(s) 386, 632, 1035
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 2 cut(s) 74, 369
MspR9I CCNGG 1 cut(s) 370
MwoI GCNNNNNNNGC 3 cut(s) 152, 745, 1026
NaeI GCCGGC 1 cut(s) 75
NarI GGCGCC 1 cut(s) 155
NciI CCSGG 1 cut(s) 370
NdeII GATC 5 cut(s) 93, 459, 646, 701, 778
NgoMIV GCCGGC 1 cut(s) 73
NlaIII CATG 3 cut(s) 16, 335, 580
NlaIV GGNNCC 2 cut(s) 156, 367
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 2 cut(s) 46, 604
NspI RCATGY 1 cut(s) 580
NspV TTCGAA 1 cut(s) 487
PaeI GCATGC 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 825
PdiI GCCGGC 1 cut(s) 75
PfeI GAWTC 3 cut(s) 173, 197, 524
PflMI CCANNNNNTGG 1 cut(s) 770
PkrI GCNGC 4 cut(s) 184, 514, 622, 841
PleI GAGTC 1 cut(s) 239
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 239
PsiI TTATAA 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 156, 367
PspPI GGNCC 2 cut(s) 356, 1028
PvuII CAGCTG 1 cut(s) 770
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 3 cut(s) 386, 632, 1035
SatI GCNGC 4 cut(s) 183, 513, 621, 840
Sau3AI GATC 5 cut(s) 93, 459, 646, 701, 778
Sau96I GGNCC 2 cut(s) 356, 1028
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 370
SduI GDGCHC 2 cut(s) 129, 750
SfaNI GCATC 2 cut(s) 797, 1019
SfcI CTRYAG 1 cut(s) 378
SfoI GGCGCC 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 487
SinI GGWCC 1 cut(s) 356
SphI GCATGC 1 cut(s) 580
SsiI CCGC 1 cut(s) 814
SspDI GGCGCC 1 cut(s) 154
SspI AATATT 1 cut(s) 1040
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 368
TaaI ACNGT 5 cut(s) 226, 382, 535, 796, 1005
TaiI ACGT 2 cut(s) 276, 876
TaqI TCGA 7 cut(s) 26, 192, 436, 442, 487, 649, 700
TfiI GAWTC 3 cut(s) 173, 197, 524
Tru1I TTAA 3 cut(s) 386, 632, 1035
Tru9I TTAA 3 cut(s) 386, 632, 1035
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 2 cut(s) 46, 604
TseI GCWGC 4 cut(s) 182, 512, 620, 839
Tsp45I GTSAC 2 cut(s) 46, 604
TspDTI ATGAA 6 cut(s) 186, 189, 579, 692, 774, 984
TspRI CASTG 1 cut(s) 39
Van91I CCANNNNNTGG 1 cut(s) 770
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 5 cut(s) 28, 188, 568, 591, 971
XceI RCATGY 1 cut(s) 580
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.