FvH4_6g01580

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010762
Physical Location & Seq
Forward (+)
897092 .. 900345
3254 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01580.t1

Sequence Viewer

Length: 1719 bp
ATGTTTGGGTGTGAGTGTTGGTACTGGAGCAGTGAATATGAGGAGCTATATGATCAGTCTGATGAGCCAAAGCCATACTCTTTGCCTTCACCTCTTCCAAAATGGCCTCAAGGCCAAAGTTTTGCATCTGGAAGAATATGCCTCGGAGAAATTGAAGTTTTTCAAGTCACCGAGTTTGAGAGTGTTTGGAGCTGCAACCGGTTGCGTGGAAAATCCAAAGCTGTCACATTTTATAGACCTGCTGGGATTCCAGATGACTTCTTTTGCCTCGGTCACTACTGTCAACCCAATGACCAGCCGTTAAGAGGTTTTCTACTTGCTGCTCGTGACATGGTTGCTACCAGGAAGGATATGGGAGATGGTTTGGTGCAAGACTTGGTACAAAAGCTACCAGCCTTAACAAAGCCCCTTAACTACTCTTTGGTGTGGAATGCAGATTCGACTAATGGTGAGTGTGGTTATATTTGGATGCCAAACCCACCTGTGGGTTATAAGGCCATGGGTTTTGTGGTCACTGACGATAGTGAGGAGCCAAGCCTTGATGTTGTTCGATGTGTACGAGAAGATCTGACAGAGACTTGTGAAGCATGTGATCAGATACTTGCAGTGGATTCGAGGTCTTCAGAGAACCAATTTCAGGTTTGGAGTACAAGACCTTGCGAACGAGGTATGTTATGTAGGAGTGTTTCAGTTGGCACATTCTTTTGCACTAATACCTACTTGGATTCTGACGAAGAACTAGAAGTTGCGTGCTTGAAGAATATTAGTTCATCCCTACACGCAATGCCAAATCTACAGCAGGTTCATACACTCATTGAGCACTATGGGCCTACTGTATTCTTCCATCCTGATGAGGCTTATTTACCATCATCAGTACAATGGTTTTTCAAAAATGGGGCAGTTTTGTGCGGTGATTCCTGTGAAAAAGGTGAACCAATTGATCATAGAGGCTCTAATTTGCCTAGTGGAGGGGAAAATTTGCATGATTATTGGATAGATTTGCCAAATGATCATGATGCAAGACATCACATCAAAGGTGGTGACATGGAGAGTGCGAAGCTATATGTTCATGTAAAACCAGCATTAGGAGGAACTTTCACCGATGTGGCCATGTGGATATTCTGTCCCTTTAATGGACCGGCCACCATTAAAGTTGGTGTACTAAGTTTTGCAATGAACAAGATAGGAGAACATGTTGGTGATTGGGAGCACTACACTCTCCGAGTGAGCAACTTCACTGGAGAACTTTGGCAAGTGTACTTCTCAGAACACAGTGGTGGTAGATGGGTGGATGCTTTTGACTTGGAGTTCATTGAAGGGAATAAGTCAGTTGTATATTCTTCAAAACATGGTCATAGTAGCTACCCTCATTCGGGAACCTATATTCAGGGGTCTTCAAAGCTTGGAATAGGATTAAAGAATGAAGTTGCTCGAAGCAAGATGTTCATTGATTCGAGCAGCAAGTACGAGATTGTTGCAGCCGAGTACCTTGGTGATGGTGTTATTGAAGAACCGTGTTGGTTGCAATATATGAGAGATTGGGGTCCAACTGTTGTGTATGATTCGCGATCAGAACTAGATAAGATAATTGATCGTCTTCCATTCTTTGTTAGATTCTCTGTGGAGAATCTATTCGAATTGTTTCCAACCGAACTCTACGGTGAGGAAGGGCCTACTGGCCCCAAGGAGAAGGATAATTGGTTGGGAGACGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

573

Amino Acids

64.64

Weight (kDa)

4.82

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 23 - 571 2.9e-266 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010762)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04350 AT3G04350
fragaria_vesca FvH4_6g01580
malus_domestica MD00G1149300.v1.1 MD04G1232300.v1.1 MD07G1000700.v1.1
prunus_persica Prupe.2G000800_v2.0.a1 Prupe.6G351900_v2.0.a1
pyrus_communis pycom04g20570 pycom12g23000
rosa_chinensis RchiOBHm_Chr3g0449321
rosa_laevigata RLG00000025839
rosa_multiflora Rmu_sc0017357.1_g000003
rosa_roxburghii Rroxscaffold_6G00427330
rosa_rugosa Rorug02G0619700
rosa_samantha Rh3AG017700 Rh3BG017800 Rh3CG016800
rosa_wichuraiana Rw3G001360 Rw3G001390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
Acc36I ACCTGC 2 cut(s) 247, 790
AccII CGCG 1 cut(s) 1567
AciI CCGC 1 cut(s) 909
AcoI YGGCCR 2 cut(s) 1107, 1140
AcsI RAATTY 1 cut(s) 976
AcuI CTGAAG 1 cut(s) 606
AfaI GTAC 9 cut(s) 23, 381, 558, 649, 876, 1161, 1259, 1466, 1487
AfiI CCNNNNNNNGG 9 cut(s) 305, 484, 485, 637, 968, 1085, 1133, 1372, 1373
AflIII ACRYGT 1 cut(s) 1192
AgeI ACCGGT 1 cut(s) 198
AgsI TTSAA 8 cut(s) 155, 164, 757, 889, 1316, 1344, 1398, 1508
AjnI CCWGG 1 cut(s) 341
AleI CACNNNNGTG 2 cut(s) 1103, 1275
AluBI AGCT 7 cut(s) 46, 192, 221, 388, 1060, 1362, 1402
AluI AGCT 7 cut(s) 46, 192, 221, 388, 1060, 1362, 1402
Alw21I GWGCWC 2 cut(s) 822, 1212
Alw26I GTCTC 2 cut(s) 569, 1701
AoxI GGCC 8 cut(s) 104, 112, 495, 827, 1107, 1140, 1670, 1678
ApeKI GCWGC 4 cut(s) 192, 320, 1458, 1478
ApoI RAATTY 1 cut(s) 976
AsiGI ACCGGT 1 cut(s) 198
Asp700I GAANNNNTTC 3 cut(s) 159, 1631, 1641
AspS9I GGNCC 5 cut(s) 827, 1136, 1544, 1670, 1679
AsuII TTCGAA 1 cut(s) 1635
AvaII GGWCC 2 cut(s) 1136, 1544
BalI TGGCCA 1 cut(s) 1109
BauI CACGAG 1 cut(s) 324
BbsI GAAGAC 3 cut(s) 612, 1386, 1589
Bbv12I GWGCWC 2 cut(s) 822, 1212
BbvI GCAGC 4 cut(s) 179, 307, 1470, 1490
BccI CCATC 5 cut(s) 353, 852, 874, 1278, 1490
BceAI ACGGC 1 cut(s) 283
BcgI CGANNNNNNTGC 4 cut(s) 594, 628, 1457, 1491
BciT130I CCWGG 1 cut(s) 343
BclI TGATCA 4 cut(s) 52, 592, 940, 1009
BcoDI GTCTC 2 cut(s) 569, 1701
BfaI CTAG 3 cut(s) 740, 963, 1577
BfmI CTRYAG 1 cut(s) 794
BfuAI ACCTGC 2 cut(s) 247, 790
BglII AGATCT 1 cut(s) 565
BisI GCNGC 4 cut(s) 193, 321, 1459, 1479
BlsI GCNGC 4 cut(s) 194, 322, 1460, 1480
Bme1390I CCNGG 1 cut(s) 343
Bme18I GGWCC 2 cut(s) 1136, 1544
BmgT120I GGNCC 5 cut(s) 827, 1136, 1544, 1670, 1679
BmiI GGNNCC 4 cut(s) 531, 1378, 1545, 1681
BmrFI CCNGG 1 cut(s) 343
BmsI GCATC 4 cut(s) 134, 459, 1006, 1282
BpiI GAAGAC 3 cut(s) 612, 1386, 1589
BpmI CTGGAG 2 cut(s) 46, 1260
Bpu14I TTCGAA 1 cut(s) 1635
BpuEI CTTGAG 1 cut(s) 93
BsaJI CCNNGG 5 cut(s) 142, 268, 498, 1489, 1683
BsaWI WCCGGW 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 1179, 1209
Bsc4I CCNNNNNNNGG 9 cut(s) 305, 484, 485, 637, 968, 1085, 1133, 1372, 1373
Bse118I RCCGGY 2 cut(s) 198, 1138
Bse1I ACTGG 3 cut(s) 29, 1243, 1681
Bse3DI GCAATG 2 cut(s) 789, 1179
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 5 cut(s) 142, 268, 498, 1489, 1683
BseGI GGATG 4 cut(s) 474, 770, 844, 1297
BseLI CCNNNNNNNGG 9 cut(s) 305, 484, 485, 637, 968, 1085, 1133, 1372, 1373
BseMI GCAATG 2 cut(s) 789, 1179
BseMII CTCAG 1 cut(s) 1278
BseNI ACTGG 3 cut(s) 29, 1243, 1681
BseRI GAGGAG 2 cut(s) 56, 542
BseXI GCAGC 4 cut(s) 179, 307, 1470, 1490
BseYI CCCAGC 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 1567
BshFI GGCC 8 cut(s) 106, 114, 497, 829, 1109, 1142, 1672, 1680
BshTI ACCGGT 1 cut(s) 198
BsiHKAI GWGCWC 2 cut(s) 822, 1212
BsiSI CCGG 2 cut(s) 199, 1139
BslFI GGGAC 1 cut(s) 1110
BslI CCNNNNNNNGG 9 cut(s) 305, 484, 485, 637, 968, 1085, 1133, 1372, 1373
BsmAI GTCTC 2 cut(s) 569, 1701
BsmBI CGTCTC 1 cut(s) 1701
BsmFI GGGAC 1 cut(s) 1110
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 8 cut(s) 106, 114, 497, 829, 1109, 1142, 1672, 1680
Bsp119I TTCGAA 1 cut(s) 1635
Bsp1286I GDGCHC 2 cut(s) 822, 1212
Bsp143I GATC 7 cut(s) 52, 565, 592, 940, 1009, 1568, 1591
Bsp19I CCATGG 1 cut(s) 498
Bsp68I TCGCGA 1 cut(s) 1567
BspACI CCGC 1 cut(s) 909
BspANI GGCC 8 cut(s) 106, 114, 497, 829, 1109, 1142, 1672, 1680
BspCNI CTCAG 1 cut(s) 1277
BspFNI CGCG 1 cut(s) 1567
BspHI TCATGA 1 cut(s) 1012
BspLI GGNNCC 4 cut(s) 531, 1378, 1545, 1681
BspMI ACCTGC 2 cut(s) 247, 790
BspT104I TTCGAA 1 cut(s) 1635
BsrDI GCAATG 2 cut(s) 789, 1179
BsrFI RCCGGY 2 cut(s) 198, 1138
BsrI ACTGG 3 cut(s) 29, 1243, 1681
BssAI RCCGGY 2 cut(s) 198, 1138
BssECI CCNNGG 5 cut(s) 142, 268, 498, 1489, 1683
BssMI GATC 7 cut(s) 52, 565, 592, 940, 1009, 1568, 1591
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 3 cut(s) 498, 1489, 1683
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 6 cut(s) 281, 835, 1274, 1515, 1552, 1661
Bst6I CTCTTC 1 cut(s) 99
BstBI TTCGAA 1 cut(s) 1635
BstC8I GCNNGC 1 cut(s) 751
BstDEI CTNAG 2 cut(s) 1163, 1264
BstDSI CCRYGG 1 cut(s) 498
BstENI CCTNNNNNAGG 1 cut(s) 966
BstF5I GGATG 4 cut(s) 474, 770, 844, 1297
BstFNI CGCG 1 cut(s) 1567
BstKTI GATC 7 cut(s) 55, 568, 595, 943, 1012, 1571, 1594
BstMAI GTCTC 2 cut(s) 569, 1701
BstMBI GATC 7 cut(s) 52, 565, 592, 940, 1009, 1568, 1591
BstMWI GCNNNNNNNGC 1 cut(s) 826
BstNI CCWGG 1 cut(s) 343
BstNSI RCATGY 2 cut(s) 591, 1196
BstSCI CCNGG 1 cut(s) 341
BstSFI CTRYAG 1 cut(s) 794
BstUI CGCG 1 cut(s) 1567
BstV1I GCAGC 4 cut(s) 179, 307, 1470, 1490
BstV2I GAAGAC 3 cut(s) 612, 1386, 1589
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BsuRI GGCC 8 cut(s) 106, 114, 497, 829, 1109, 1142, 1672, 1680
BtgI CCRYGG 1 cut(s) 498
BtsCI GGATG 4 cut(s) 474, 770, 844, 1297
BtsI GCAGTG 2 cut(s) 37, 612
BtsIMutI CAGTG 5 cut(s) 37, 513, 612, 1236, 1279
BtuMI TCGCGA 1 cut(s) 1567
BveI ACCTGC 2 cut(s) 247, 790
Cac8I GCNNGC 1 cut(s) 751
CciI TCATGA 1 cut(s) 1012
Cfr10I RCCGGY 2 cut(s) 198, 1138
Cfr13I GGNCC 5 cut(s) 827, 1136, 1544, 1670, 1679
Csp6I GTAC 9 cut(s) 22, 380, 557, 648, 875, 1160, 1258, 1465, 1486
CspAI ACCGGT 1 cut(s) 198
CviQI GTAC 9 cut(s) 22, 380, 557, 648, 875, 1160, 1258, 1465, 1486
DdeI CTNAG 2 cut(s) 1163, 1264
DpnI GATC 7 cut(s) 54, 567, 594, 942, 1011, 1570, 1593
DpnII GATC 7 cut(s) 52, 565, 592, 940, 1009, 1568, 1591
EaeI YGGCCR 2 cut(s) 1107, 1140
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco130I CCWWGG 3 cut(s) 498, 1489, 1683
Eco47I GGWCC 2 cut(s) 1136, 1544
Eco57I CTGAAG 1 cut(s) 606
EcoNI CCTNNNNNAGG 1 cut(s) 966
EcoO109I RGGNCCY 1 cut(s) 1670
EcoRII CCWGG 1 cut(s) 341
EcoT14I CCWWGG 3 cut(s) 498, 1489, 1683
ErhI CCWWGG 3 cut(s) 498, 1489, 1683
Esp3I CGTCTC 1 cut(s) 1701
FaqI GGGAC 1 cut(s) 1110
FbaI TGATCA 4 cut(s) 52, 592, 940, 1009
Fnu4HI GCNGC 4 cut(s) 193, 321, 1459, 1479
FokI GGATG 4 cut(s) 481, 757, 831, 1304
Fsp4HI GCNGC 4 cut(s) 193, 321, 1459, 1479
FspBI CTAG 3 cut(s) 740, 963, 1577
GluI GCNGC 4 cut(s) 193, 321, 1459, 1479
GsaI CCCAGC 1 cut(s) 246
GsuI CTGGAG 2 cut(s) 46, 1260
HaeIII GGCC 8 cut(s) 106, 114, 497, 829, 1109, 1142, 1672, 1680
HapII CCGG 2 cut(s) 199, 1139
HincII GTYRAC 1 cut(s) 284
HindII GTYRAC 1 cut(s) 284
HindIII AAGCTT 1 cut(s) 1400
HinfI GANTC 9 cut(s) 247, 437, 611, 725, 914, 1451, 1562, 1614, 1627
HpaII CCGG 2 cut(s) 199, 1139
Hpy166II GTNNAC 5 cut(s) 284, 557, 932, 1160, 1258
Hpy188I TCNGA 9 cut(s) 61, 146, 570, 597, 625, 730, 1223, 1267, 1573
Hpy188III TCNNGA 7 cut(s) 129, 251, 326, 848, 1013, 1374, 1566
Hpy8I GTNNAC 5 cut(s) 284, 557, 932, 1160, 1258
HpyAV CCTTC 5 cut(s) 96, 340, 1310, 1661, 1684
HpyCH4III ACNGT 6 cut(s) 281, 835, 1274, 1515, 1552, 1661
HpyF10VI GCNNNNNNNGC 1 cut(s) 826
HpyF3I CTNAG 2 cut(s) 1163, 1264
Ksp22I TGATCA 4 cut(s) 52, 592, 940, 1009
Kzo9I GATC 7 cut(s) 52, 565, 592, 940, 1009, 1568, 1591
LmnI GCTCC 5 cut(s) 27, 43, 189, 529, 1207
Lsp1109I GCAGC 4 cut(s) 179, 307, 1470, 1490
LweI GCATC 4 cut(s) 134, 459, 1006, 1282
MaeI CTAG 3 cut(s) 740, 963, 1577
MaeIII GTNAC 6 cut(s) 166, 223, 272, 326, 511, 1040
MalI GATC 7 cut(s) 54, 567, 594, 942, 1011, 1570, 1593
MboI GATC 7 cut(s) 52, 565, 592, 940, 1009, 1568, 1591
MfeI CAATTG 1 cut(s) 936
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 2 cut(s) 822, 1212
MlsI TGGCCA 1 cut(s) 1109
MluCI AATT 8 cut(s) 150, 632, 936, 955, 976, 1587, 1637, 1696
MluNI TGGCCA 1 cut(s) 1109
MmeI TCCRAC 2 cut(s) 1571, 1670
Mox20I TGGCCA 1 cut(s) 1109
MroXI GAANNNNTTC 3 cut(s) 159, 1631, 1641
MscI TGGCCA 1 cut(s) 1109
MseI TTAA 6 cut(s) 302, 398, 411, 1131, 1149, 1415
MslI CAYNNNNRTG 4 cut(s) 849, 1103, 1197, 1275
Msp20I TGGCCA 1 cut(s) 1109
MspI CCGG 2 cut(s) 199, 1139
MspR9I CCNGG 1 cut(s) 343
MunI CAATTG 1 cut(s) 936
Mva1269I GAATGC 1 cut(s) 436
MvaI CCWGG 1 cut(s) 343
MvnI CGCG 1 cut(s) 1567
MwoI GCNNNNNNNGC 1 cut(s) 826
NcoI CCATGG 1 cut(s) 498
NdeII GATC 7 cut(s) 52, 565, 592, 940, 1009, 1568, 1591
NlaIV GGNNCC 4 cut(s) 531, 1378, 1545, 1681
NmeAIII GCCGAG 1 cut(s) 1507
NmuCI GTSAC 6 cut(s) 166, 223, 272, 326, 511, 1040
NruI TCGCGA 1 cut(s) 1567
NspI RCATGY 2 cut(s) 591, 1196
NspV TTCGAA 1 cut(s) 1635
OliI CACNNNNGTG 2 cut(s) 1103, 1275
PagI TCATGA 1 cut(s) 1012
PciI ACATGT 1 cut(s) 1192
PcsI WCGNNNNNNNCGW 1 cut(s) 556
PctI GAATGC 1 cut(s) 436
PdmI GAANNNNTTC 3 cut(s) 159, 1631, 1641
PfeI GAWTC 9 cut(s) 247, 437, 611, 725, 914, 1451, 1562, 1614, 1627
PinAI ACCGGT 1 cut(s) 198
PkrI GCNGC 4 cut(s) 194, 322, 1460, 1480
PscI ACATGT 1 cut(s) 1192
PsiI TTATAA 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 341
PspFI CCCAGC 1 cut(s) 242
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 4 cut(s) 531, 1378, 1545, 1681
PspPI GGNCC 5 cut(s) 827, 1136, 1544, 1670, 1679
PsuI RGATCY 1 cut(s) 565
RruI TCGCGA 1 cut(s) 1567
RsaI GTAC 9 cut(s) 23, 381, 558, 649, 876, 1161, 1259, 1466, 1487
RsaNI GTAC 9 cut(s) 22, 380, 557, 648, 875, 1160, 1258, 1465, 1486
RseI CAYNNNNRTG 4 cut(s) 849, 1103, 1197, 1275
SaqAI TTAA 6 cut(s) 302, 398, 411, 1131, 1149, 1415
SatI GCNGC 4 cut(s) 193, 321, 1459, 1479
Sau3AI GATC 7 cut(s) 52, 565, 592, 940, 1009, 1568, 1591
Sau96I GGNCC 5 cut(s) 827, 1136, 1544, 1670, 1679
ScrFI CCNGG 1 cut(s) 343
SduI GDGCHC 2 cut(s) 822, 1212
SfaNI GCATC 4 cut(s) 134, 459, 1006, 1282
SfcI CTRYAG 1 cut(s) 794
SfuI TTCGAA 1 cut(s) 1635
SinI GGWCC 2 cut(s) 1136, 1544
SmiMI CAYNNNNRTG 4 cut(s) 849, 1103, 1197, 1275
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
Sse9I AATT 8 cut(s) 150, 632, 936, 955, 976, 1587, 1637, 1696
SsiI CCGC 1 cut(s) 909
SspI AATATT 1 cut(s) 763
SspMI CTAG 3 cut(s) 740, 963, 1577
StyD4I CCNGG 1 cut(s) 341
StyI CCWWGG 3 cut(s) 498, 1489, 1683
TaaI ACNGT 6 cut(s) 281, 835, 1274, 1515, 1552, 1661
TaqI TCGA 6 cut(s) 440, 550, 614, 1432, 1454, 1635
TaqII GACCGA 1 cut(s) 260
TasI AATT 8 cut(s) 150, 632, 936, 955, 976, 1587, 1637, 1696
TatI WGTACW 4 cut(s) 647, 874, 1159, 1257
TfiI GAWTC 9 cut(s) 247, 437, 611, 725, 914, 1451, 1562, 1614, 1627
Tru1I TTAA 6 cut(s) 302, 398, 411, 1131, 1149, 1415
Tru9I TTAA 6 cut(s) 302, 398, 411, 1131, 1149, 1415
TscAI CASTG 5 cut(s) 37, 520, 612, 1243, 1279
TseFI GTSAC 6 cut(s) 166, 223, 272, 326, 511, 1040
TseI GCWGC 4 cut(s) 192, 320, 1458, 1478
Tsp45I GTSAC 6 cut(s) 166, 223, 272, 326, 511, 1040
TspDTI ATGAA 7 cut(s) 759, 794, 1058, 1190, 1300, 1435, 1437
TspRI CASTG 5 cut(s) 37, 520, 612, 1243, 1279
VpaK11BI GGWCC 2 cut(s) 1136, 1544
XagI CCTNNNNNAGG 1 cut(s) 966
XapI RAATTY 1 cut(s) 976
XceI RCATGY 2 cut(s) 591, 1196
XcmI CCANNNNNNNNNTGG 2 cut(s) 349, 505
XmnI GAANNNNTTC 3 cut(s) 159, 1631, 1641
XspI CTAG 3 cut(s) 740, 963, 1577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.