pycom12g23000

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010762
Physical Location & Seq
Reverse (-)
23711507 .. 23714588
3082 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23000.2

Sequence Viewer

Length: 1716 bp
ATGTTGGGGTGTGAGTGCTGGTGGTGGAGGAGCAGTGACTACCAATTGTATGATTACCCAGAGCCGCAGCCGCAGCCGCAGCAGCCCTTCTCTCTGCCTTCACCTCTTCCACACTGGCCTCAAGGCGAAGGATTTGCTACTGGAAGAATACGCCTCGGAGAAATTGAAGTTATCCAAGTCACCAAGTTTGAGAGCATCTGGAGCTGTAGTCTGTTGCGTGGCAAATCAAAGGGTGTCACCTTTTATAGACCTGCAGGGATCCCCGATGACTTCTTTTGCCTTGGTTACTACTGCCAGCAGAATGACCAGCCATTGAGAGGGTATGTTCTTGTGGTGCGTGAAACGGTTGCCAAAAGGGTGGAAGATGGTTGTACTCGAGGCTCAGCATTAGAGTTACCGGCTTTAAAAAAGCCGGTTAACTACTCATTAGTCTGGAATGCGGATTTAAGTAATAATGGTTGTGGTTATATTTGGTTGCCAAACCCACCTGTTGGTTACAAGGCCATGGGTTTTGTGGTCACTGACAAGCCTGAGGAACCCAGCCCTGAAGAAGTTAGGTGCATTCGAGAAGATCTTACAGAGACTTGTGAAACACATGATCGGATACTTGCCATGGATTCAAAGTTGGACCAATTTCAGGTGTGGAATACAAGACCCTGCAAACGAGGTATGTTCTGCAGTGGTGTTTCGGTTGGTACATTCTTTTGCAGTACCTACTTGGATTCCGATGAAGAGCTAGAAGTTGCGTGCTTGAAGAATTTTGATCCGTCCTTACACGCAATGCCAAATCTGAACCAGATTCATTCACTAATTGAGCACTACGGCCCCACAGTGTTCTTCCATCCTAATGAGGTTTATTTGCCATCATCAGTACAATGGTTTTTCAAAAACGGGGCACTTTTGTACCGGGAAGACACTGCGAATGGCGAACCGATTGATTACAGAGGCTCCAATTTGCCTGCTGGAGGGGAAAATGATAGTGATTTTTGGATAGATTTGCCAAGCGATGATGAAGCTAAAAATCTTCTCAAGGCCGGCAACATAGACAGTGCGGAGCTATACGTTCATGTAAAACCAGCATTGGGAGGAACTTTTACAGATATTGCTATGTGGGTGTTCTGCCCCTTCAATGGACCGTCCACCATTAAAATTGGTTTGGTAAATATTGCACTGAACAAGATAGGCGAACATGTCGGTGACTGGGAGCACTTCACTCTCCGAGTGAACAACTTCACGGGAGAACTCTGGCAAGTGTATTTCTCAGAGCACAGCGGTGGCAGATGGGTGGATGCTTTTGACCTGGAGTTCATCGAAGGGAGTAAGCCGATTGTGTACTCATCAAAACACGGTCATAGTAGCTATGCTCATCCCGGGACGTACCTCCAGGGTTCATCCAAGTTTGACATAGGAGTAAGGAATGATGCTGCGCGGAGCAAGTTCTTCATTGATTCGAGCACCAAGTATCAAATTGTTGCGGCCGAGTACCTTGGCAATGGTGTTATATCCGAACCATGTTGGTTGCAGTATATGCGAGACTGGGGTCCGACCATTGTGTATGATGGACGATCGGAACTAGATAAGTTGATCGATCGCCTTCCGTTCTTTGTTCGATTCTCTGTCGAGAACTTAATCGAGTTGTTTCCTACTCAAATCTACGGTGAGGAAGGGCCAACTGGCCCTAAGGAGAAGGATAATTGGGTAGGAGATGAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

572

Amino Acids

64.72

Weight (kDa)

4.91

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010762)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04350 AT3G04350
fragaria_vesca FvH4_6g01580
malus_domestica MD00G1149300.v1.1 MD04G1232300.v1.1 MD07G1000700.v1.1
prunus_persica Prupe.2G000800_v2.0.a1 Prupe.6G351900_v2.0.a1
pyrus_communis pycom04g20570 pycom12g23000
rosa_chinensis RchiOBHm_Chr3g0449321
rosa_laevigata RLG00000025839
rosa_multiflora Rmu_sc0017357.1_g000003
rosa_roxburghii Rroxscaffold_6G00427330
rosa_rugosa Rorug02G0619700
rosa_samantha Rh3AG017700 Rh3BG017800 Rh3CG016800
rosa_wichuraiana Rw3G001360 Rw3G001390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 259
AccB7I CCANNNNNTGG 1 cut(s) 491
AccII CGCG 1 cut(s) 1429
AciI CCGC 8 cut(s) 65, 71, 77, 440, 1052, 1272, 1429, 1475
AclWI GGATC 3 cut(s) 253, 266, 758
AcoI YGGCCR 1 cut(s) 1476
AcsI RAATTY 1 cut(s) 757
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 8 cut(s) 373, 697, 712, 873, 905, 1334, 1379, 1484
AfiI CCNNNNNNNGG 6 cut(s) 317, 491, 637, 965, 1082, 1130
AflIII ACRYGT 1 cut(s) 1189
AgsI TTSAA 5 cut(s) 167, 621, 754, 886, 1129
AjnI CCWGG 2 cut(s) 1299, 1383
AleI CACNNNNGTG 1 cut(s) 1272
AluBI AGCT 5 cut(s) 204, 736, 1016, 1057, 1359
AluI AGCT 5 cut(s) 204, 736, 1016, 1057, 1359
Alw21I GWGCWC 4 cut(s) 819, 1209, 1269, 1457
Alw26I GTCTC 2 cut(s) 575, 1527
AlwI GGATC 3 cut(s) 253, 266, 758
Ama87I CYCGRG 2 cut(s) 375, 1370
AoxI GGCC 7 cut(s) 116, 501, 823, 1032, 1476, 1667, 1675
ApeKI GCWGC 5 cut(s) 67, 73, 79, 82, 1424
ApoI RAATTY 1 cut(s) 757
Asp700I GAANNNNTTC 1 cut(s) 1229
AspLEI GCGC 1 cut(s) 1429
AspS9I GGNCC 6 cut(s) 628, 824, 1133, 1541, 1667, 1676
AsuC2I CCSGG 3 cut(s) 908, 1371, 1372
AsuHPI GGTGA 5 cut(s) 93, 172, 229, 1208, 1670
AvaI CYCGRG 2 cut(s) 375, 1370
AvaII GGWCC 3 cut(s) 628, 1133, 1541
AxyI CCTNAGG 2 cut(s) 531, 1680
BaeGI GKGCMC 1 cut(s) 898
BaeI ACNNNNGTAYC 2 cut(s) 887, 920
BamHI GGATCC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 918
Bbv12I GWGCWC 4 cut(s) 819, 1209, 1269, 1457
BbvI GCAGC 5 cut(s) 79, 85, 91, 94, 1411
BccI CCATC 5 cut(s) 359, 849, 871, 1275, 1553
BceAI ACGGC 1 cut(s) 838
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 2 cut(s) 1301, 1385
BciVI GTATCC 1 cut(s) 597
BcnI CCSGG 3 cut(s) 908, 1371, 1372
BcoDI GTCTC 2 cut(s) 575, 1527
BfaI CTAG 2 cut(s) 737, 1574
BfmI CTRYAG 3 cut(s) 205, 252, 676
BfuAI ACCTGC 1 cut(s) 259
BfuI GTATCC 1 cut(s) 597
BglII AGATCT 1 cut(s) 571
BisI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 83, 1425, 1476
BlpI GCTNAGC 1 cut(s) 382
BlsI GCNGC 9 cut(s) 66, 69, 72, 75, 78, 81, 84, 1426, 1477
Bme1390I CCNGG 5 cut(s) 908, 1301, 1371, 1372, 1385
Bme18I GGWCC 3 cut(s) 628, 1133, 1541
BmeT110I CYCGRG 2 cut(s) 375, 1370
BmgT120I GGNCC 6 cut(s) 628, 824, 1133, 1541, 1667, 1676
BmiI GGNNCC 5 cut(s) 260, 537, 826, 949, 1542
BmrFI CCNGG 5 cut(s) 908, 1301, 1371, 1372, 1385
BmrI ACTGGG 2 cut(s) 1210, 1546
BmsI GCATC 3 cut(s) 204, 1279, 1411
BmuI ACTGGG 2 cut(s) 1210, 1546
BoxI GACNNNNGTC 1 cut(s) 1539
BpiI GAAGAC 1 cut(s) 918
BpmI CTGGAG 4 cut(s) 220, 984, 1322, 1367
Bpu1102I GCTNAGC 1 cut(s) 382
BpuEI CTTGAG 2 cut(s) 105, 1013
BpuMI CCSGG 3 cut(s) 908, 1371, 1372
Bsa29I ATCGAT 1 cut(s) 1587
BsaJI CCNNGG 7 cut(s) 154, 280, 504, 612, 1370, 1384, 1486
BsaXI ACNNNNNCTCC 6 cut(s) 19, 49, 193, 223, 932, 962
Bsc4I CCNNNNNNNGG 6 cut(s) 317, 491, 637, 965, 1082, 1130
Bse118I RCCGGY 3 cut(s) 397, 412, 1034
Bse1I ACTGG 5 cut(s) 119, 145, 1205, 1541, 1678
Bse21I CCTNAGG 2 cut(s) 531, 1680
Bse3DI GCAATG 2 cut(s) 786, 1498
BseBI CCWGG 2 cut(s) 1301, 1385
BseCI ATCGAT 1 cut(s) 1587
BseDI CCNNGG 7 cut(s) 154, 280, 504, 612, 1370, 1384, 1486
BseGI GGATG 4 cut(s) 841, 1294, 1366, 1391
BseLI CCNNNNNNNGG 6 cut(s) 317, 491, 637, 965, 1082, 1130
BseMI GCAATG 2 cut(s) 786, 1498
BseMII CTCAG 3 cut(s) 396, 522, 1275
BseNI ACTGG 5 cut(s) 119, 145, 1205, 1541, 1678
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 1 cut(s) 898
BseX3I CGGCCG 1 cut(s) 1476
BseXI GCAGC 5 cut(s) 79, 85, 91, 94, 1411
BseYI CCCAGC 1 cut(s) 539
Bsh1236I CGCG 1 cut(s) 1429
Bsh1285I CGRYCG 3 cut(s) 1479, 1568, 1591
BshFI GGCC 7 cut(s) 118, 503, 825, 1034, 1478, 1669, 1677
BshVI ATCGAT 1 cut(s) 1587
BsiEI CGRYCG 3 cut(s) 1479, 1568, 1591
BsiHKAI GWGCWC 4 cut(s) 819, 1209, 1269, 1457
BsiHKCI CYCGRG 2 cut(s) 375, 1370
BsiSI CCGG 5 cut(s) 398, 413, 907, 1035, 1371
BslFI GGGAC 1 cut(s) 1387
BslI CCNNNNNNNGG 6 cut(s) 317, 491, 637, 965, 1082, 1130
BsmAI GTCTC 2 cut(s) 575, 1527
BsmFI GGGAC 1 cut(s) 1387
BsmI GAATGC 2 cut(s) 442, 561
BsnI GGCC 7 cut(s) 118, 503, 825, 1034, 1478, 1669, 1677
BsoBI CYCGRG 2 cut(s) 375, 1370
Bsp1286I GDGCHC 5 cut(s) 819, 898, 1209, 1269, 1457
Bsp143I GATC 7 cut(s) 258, 571, 598, 763, 1565, 1584, 1588
Bsp1720I GCTNAGC 1 cut(s) 382
Bsp19I CCATGG 2 cut(s) 504, 612
BspACI CCGC 8 cut(s) 65, 71, 77, 440, 1052, 1272, 1429, 1475
BspANI GGCC 7 cut(s) 118, 503, 825, 1034, 1478, 1669, 1677
BspCNI CTCAG 3 cut(s) 395, 523, 1274
BspDI ATCGAT 1 cut(s) 1587
BspFNI CGCG 1 cut(s) 1429
BspLI GGNNCC 5 cut(s) 260, 537, 826, 949, 1542
BspMAI CTGCAG 2 cut(s) 256, 680
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 3 cut(s) 253, 266, 758
BspQI GCTCTTC 1 cut(s) 726
BsrDI GCAATG 2 cut(s) 786, 1498
BsrFI RCCGGY 3 cut(s) 397, 412, 1034
BsrI ACTGG 5 cut(s) 119, 145, 1205, 1541, 1678
BssAI RCCGGY 3 cut(s) 397, 412, 1034
BssECI CCNNGG 7 cut(s) 154, 280, 504, 612, 1370, 1384, 1486
BssMI GATC 7 cut(s) 258, 571, 598, 763, 1565, 1584, 1588
BssT1I CCWWGG 4 cut(s) 280, 504, 612, 1486
Bst2UI CCWGG 2 cut(s) 1301, 1385
Bst4CI ACNGT 6 cut(s) 346, 832, 1049, 1137, 1349, 1658
Bst6I CTCTTC 2 cut(s) 111, 726
BstAPI GCANNNNNTGC 1 cut(s) 1528
BstC8I GCNNGC 4 cut(s) 296, 748, 960, 1036
BstDEI CTNAG 4 cut(s) 382, 531, 1261, 1680
BstDSI CCRYGG 2 cut(s) 504, 612
BstENI CCTNNNNNAGG 1 cut(s) 963
BstF5I GGATG 4 cut(s) 841, 1294, 1366, 1391
BstFNI CGCG 1 cut(s) 1429
BstHHI GCGC 1 cut(s) 1429
BstKTI GATC 7 cut(s) 261, 574, 601, 766, 1568, 1587, 1591
BstMAI GTCTC 2 cut(s) 575, 1527
BstMBI GATC 7 cut(s) 258, 571, 598, 763, 1565, 1584, 1588
BstMCI CGRYCG 3 cut(s) 1479, 1568, 1591
BstMWI GCNNNNNNNGC 7 cut(s) 70, 73, 76, 79, 82, 201, 1528
BstNI CCWGG 2 cut(s) 1301, 1385
BstNSI RCATGY 1 cut(s) 1193
BstPAI GACNNNNGTC 1 cut(s) 1539
BstSCI CCNGG 5 cut(s) 906, 1299, 1369, 1370, 1383
BstSFI CTRYAG 3 cut(s) 205, 252, 676
BstSLI GKGCMC 1 cut(s) 898
BstUI CGCG 1 cut(s) 1429
BstV1I GCAGC 5 cut(s) 79, 85, 91, 94, 1411
BstV2I GAAGAC 1 cut(s) 918
BstX2I RGATCY 2 cut(s) 258, 571
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 2 cut(s) 258, 571
BstZI CGGCCG 1 cut(s) 1476
Bsu15I ATCGAT 1 cut(s) 1587
Bsu36I CCTNAGG 2 cut(s) 531, 1680
BsuI GTATCC 1 cut(s) 597
BsuRI GGCC 7 cut(s) 118, 503, 825, 1034, 1478, 1669, 1677
BsuTUI ATCGAT 1 cut(s) 1587
BtgI CCRYGG 2 cut(s) 504, 612
BtgZI GCGATG 1 cut(s) 1020
BtsCI GGATG 4 cut(s) 841, 1294, 1366, 1391
BtsI GCAGTG 3 cut(s) 40, 685, 915
BtsIMutI CAGTG 8 cut(s) 40, 112, 519, 685, 837, 915, 1054, 1169
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 4 cut(s) 296, 748, 960, 1036
CfoI GCGC 1 cut(s) 1429
Cfr10I RCCGGY 3 cut(s) 397, 412, 1034
Cfr13I GGNCC 6 cut(s) 628, 824, 1133, 1541, 1667, 1676
Cfr9I CCCGGG 1 cut(s) 1370
ClaI ATCGAT 1 cut(s) 1587
Csp6I GTAC 8 cut(s) 372, 696, 711, 872, 904, 1333, 1378, 1483
CviAII CATG 6 cut(s) 505, 596, 613, 1067, 1190, 1512
CviQI GTAC 8 cut(s) 372, 696, 711, 872, 904, 1333, 1378, 1483
DdeI CTNAG 4 cut(s) 382, 531, 1261, 1680
DpnI GATC 7 cut(s) 260, 573, 600, 765, 1567, 1586, 1590
DpnII GATC 7 cut(s) 258, 571, 598, 763, 1565, 1584, 1588
DraI TTTAAA 1 cut(s) 405
EaeI YGGCCR 1 cut(s) 1476
EagI CGGCCG 1 cut(s) 1476
Eam1104I CTCTTC 2 cut(s) 111, 726
EarI CTCTTC 2 cut(s) 111, 726
EclXI CGGCCG 1 cut(s) 1476
Eco130I CCWWGG 4 cut(s) 280, 504, 612, 1486
Eco47I GGWCC 3 cut(s) 628, 1133, 1541
Eco52I CGGCCG 1 cut(s) 1476
Eco57I CTGAAG 1 cut(s) 567
Eco81I CCTNAGG 2 cut(s) 531, 1680
Eco88I CYCGRG 2 cut(s) 375, 1370
EcoNI CCTNNNNNAGG 1 cut(s) 963
EcoRII CCWGG 2 cut(s) 1299, 1383
EcoT14I CCWWGG 4 cut(s) 280, 504, 612, 1486
ErhI CCWWGG 4 cut(s) 280, 504, 612, 1486
FaeI CATG 6 cut(s) 508, 599, 616, 1070, 1193, 1515
FaqI GGGAC 1 cut(s) 1387
FatI CATG 6 cut(s) 504, 595, 612, 1066, 1189, 1511
Fnu4HI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 83, 1425, 1476
FokI GGATG 4 cut(s) 828, 1301, 1353, 1378
Fsp4HI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 83, 1425, 1476
FspBI CTAG 2 cut(s) 737, 1574
GlaI GCGC 1 cut(s) 1428
GluI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 83, 1425, 1476
GsaI CCCAGC 1 cut(s) 543
GsuI CTGGAG 4 cut(s) 220, 984, 1322, 1367
HaeIII GGCC 7 cut(s) 118, 503, 825, 1034, 1478, 1669, 1677
HapII CCGG 5 cut(s) 398, 413, 907, 1035, 1371
HhaI GCGC 1 cut(s) 1429
Hin1II CATG 6 cut(s) 508, 599, 616, 1070, 1193, 1515
Hin6I GCGC 1 cut(s) 1427
HinP1I GCGC 1 cut(s) 1427
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 5 cut(s) 617, 722, 799, 1448, 1611
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 5 cut(s) 398, 413, 907, 1035, 1371
HphI GGTGA 5 cut(s) 93, 172, 229, 1208, 1670
Hpy166II GTNNAC 4 cut(s) 418, 1140, 1225, 1333
Hpy188I TCNGA 9 cut(s) 158, 603, 727, 792, 1220, 1264, 1507, 1545, 1570
Hpy188III TCNNGA 4 cut(s) 199, 433, 566, 1621
Hpy8I GTNNAC 4 cut(s) 418, 1140, 1225, 1333
HpyAV CCTTC 8 cut(s) 97, 108, 122, 1135, 1307, 1604, 1658, 1681
HpyCH4III ACNGT 6 cut(s) 346, 832, 1049, 1137, 1349, 1658
HpyCH4IV ACGT 2 cut(s) 1062, 1376
HpyCH4V TGCA 7 cut(s) 254, 561, 660, 678, 708, 1169, 1522
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 73, 76, 79, 82, 201, 1528
HpyF3I CTNAG 4 cut(s) 382, 531, 1261, 1680
HpySE526I ACGT 2 cut(s) 1062, 1376
Hsp92II CATG 6 cut(s) 508, 599, 616, 1070, 1193, 1515
HspAI GCGC 1 cut(s) 1427
KroI GCCGGC 1 cut(s) 1034
KroNI GCCGGC 1 cut(s) 1036
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 7 cut(s) 258, 571, 598, 763, 1565, 1584, 1588
LguI GCTCTTC 1 cut(s) 726
LmnI GCTCC 6 cut(s) 30, 201, 953, 1054, 1204, 1431
Lsp1109I GCAGC 5 cut(s) 79, 85, 91, 94, 1411
LweI GCATC 3 cut(s) 204, 1279, 1411
MaeI CTAG 2 cut(s) 737, 1574
MaeII ACGT 2 cut(s) 1062, 1376
MaeIII GTNAC 8 cut(s) 35, 178, 235, 284, 393, 494, 517, 1196
MalI GATC 7 cut(s) 260, 573, 600, 765, 1567, 1586, 1590
MboI GATC 7 cut(s) 258, 571, 598, 763, 1565, 1584, 1588
MfeI CAATTG 1 cut(s) 44
MflI RGATCY 2 cut(s) 258, 571
MhlI GDGCHC 5 cut(s) 819, 898, 1209, 1269, 1457
MluCI AATT 9 cut(s) 44, 162, 632, 757, 810, 952, 1149, 1467, 1693
MmeI TCCRAC 2 cut(s) 606, 1568
MroNI GCCGGC 1 cut(s) 1034
MroXI GAANNNNTTC 1 cut(s) 1229
MseI TTAA 5 cut(s) 404, 417, 446, 1146, 1628
MslI CAYNNNNRTG 3 cut(s) 846, 1194, 1272
MspA1I CMGCKG 1 cut(s) 1272
MspI CCGG 5 cut(s) 398, 413, 907, 1035, 1371
MspR9I CCNGG 5 cut(s) 908, 1301, 1371, 1372, 1385
MunI CAATTG 1 cut(s) 44
Mva1269I GAATGC 2 cut(s) 442, 561
MvaI CCWGG 2 cut(s) 1301, 1385
MvnI CGCG 1 cut(s) 1429
MwoI GCNNNNNNNGC 7 cut(s) 70, 73, 76, 79, 82, 201, 1528
NaeI GCCGGC 1 cut(s) 1036
NciI CCSGG 3 cut(s) 908, 1371, 1372
NcoI CCATGG 2 cut(s) 504, 612
NdeII GATC 7 cut(s) 258, 571, 598, 763, 1565, 1584, 1588
NgoMIV GCCGGC 1 cut(s) 1034
NlaIII CATG 6 cut(s) 508, 599, 616, 1070, 1193, 1515
NlaIV GGNNCC 5 cut(s) 260, 537, 826, 949, 1542
NmeAIII GCCGAG 1 cut(s) 1504
NmuCI GTSAC 5 cut(s) 35, 178, 235, 517, 1196
NspI RCATGY 1 cut(s) 1193
OliI CACNNNNGTG 1 cut(s) 1272
PaeR7I CTCGAG 1 cut(s) 375
PciI ACATGT 1 cut(s) 1189
PciSI GCTCTTC 1 cut(s) 726
PctI GAATGC 2 cut(s) 442, 561
PdiI GCCGGC 1 cut(s) 1036
PdmI GAANNNNTTC 1 cut(s) 1229
PfeI GAWTC 5 cut(s) 617, 722, 799, 1448, 1611
PflMI CCANNNNNTGG 1 cut(s) 491
PkrI GCNGC 9 cut(s) 66, 69, 72, 75, 78, 81, 84, 1426, 1477
Ple19I CGATCG 2 cut(s) 1568, 1591
PscI ACATGT 1 cut(s) 1189
PshAI GACNNNNGTC 1 cut(s) 1539
Psp6I CCWGG 2 cut(s) 1299, 1383
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 2 cut(s) 1299, 1383
PspN4I GGNNCC 5 cut(s) 260, 537, 826, 949, 1542
PspPI GGNCC 6 cut(s) 628, 824, 1133, 1541, 1667, 1676
PspXI VCTCGAGB 1 cut(s) 375
PstI CTGCAG 2 cut(s) 256, 680
PsuI RGATCY 2 cut(s) 258, 571
PvuI CGATCG 2 cut(s) 1568, 1591
RsaI GTAC 8 cut(s) 373, 697, 712, 873, 905, 1334, 1379, 1484
RsaNI GTAC 8 cut(s) 372, 696, 711, 872, 904, 1333, 1378, 1483
RseI CAYNNNNRTG 3 cut(s) 846, 1194, 1272
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 5 cut(s) 404, 417, 446, 1146, 1628
SatI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 83, 1425, 1476
Sau3AI GATC 7 cut(s) 258, 571, 598, 763, 1565, 1584, 1588
Sau96I GGNCC 6 cut(s) 628, 824, 1133, 1541, 1667, 1676
SbfI CCTGCAGG 1 cut(s) 256
ScrFI CCNGG 5 cut(s) 908, 1301, 1371, 1372, 1385
SdaI CCTGCAGG 1 cut(s) 256
SduI GDGCHC 5 cut(s) 819, 898, 1209, 1269, 1457
SfaNI GCATC 3 cut(s) 204, 1279, 1411
SfcI CTRYAG 3 cut(s) 205, 252, 676
Sfr274I CTCGAG 1 cut(s) 375
SinI GGWCC 3 cut(s) 628, 1133, 1541
SlaI CTCGAG 1 cut(s) 375
SmaI CCCGGG 1 cut(s) 1372
SmiMI CAYNNNNRTG 3 cut(s) 846, 1194, 1272
SmlI CTYRAG 3 cut(s) 120, 375, 1028
SmoI CTYRAG 3 cut(s) 120, 375, 1028
Sse8387I CCTGCAGG 1 cut(s) 256
Sse9I AATT 9 cut(s) 44, 162, 632, 757, 810, 952, 1149, 1467, 1693
SsiI CCGC 8 cut(s) 65, 71, 77, 440, 1052, 1272, 1429, 1475
SspI AATATT 1 cut(s) 1165
SspMI CTAG 2 cut(s) 737, 1574
StyD4I CCNGG 5 cut(s) 906, 1299, 1369, 1370, 1383
StyI CCWWGG 4 cut(s) 280, 504, 612, 1486
TaaI ACNGT 6 cut(s) 346, 832, 1049, 1137, 1349, 1658
TaiI ACGT 2 cut(s) 1065, 1379
TaqI TCGA 8 cut(s) 376, 565, 1311, 1451, 1587, 1609, 1620, 1632
TasI AATT 9 cut(s) 44, 162, 632, 757, 810, 952, 1149, 1467, 1693
TatI WGTACW 3 cut(s) 371, 871, 1332
TauI GCSGC 4 cut(s) 67, 73, 79, 1478
TfiI GAWTC 5 cut(s) 617, 722, 799, 1448, 1611
Tru1I TTAA 5 cut(s) 404, 417, 446, 1146, 1628
Tru9I TTAA 5 cut(s) 404, 417, 446, 1146, 1628
TscAI CASTG 8 cut(s) 40, 119, 526, 685, 837, 922, 1054, 1176
TseFI GTSAC 5 cut(s) 35, 178, 235, 517, 1196
TseI GCWGC 5 cut(s) 67, 73, 79, 82, 1424
Tsp45I GTSAC 5 cut(s) 35, 178, 235, 517, 1196
TspDTI ATGAA 7 cut(s) 744, 791, 1026, 1055, 1297, 1380, 1432
TspGWI ACGGA 2 cut(s) 756, 1587
TspMI CCCGGG 1 cut(s) 1370
TspRI CASTG 8 cut(s) 40, 119, 526, 685, 837, 922, 1054, 1176
Van91I CCANNNNNTGG 1 cut(s) 491
VpaK11BI GGWCC 3 cut(s) 628, 1133, 1541
XagI CCTNNNNNAGG 1 cut(s) 963
XapI RAATTY 1 cut(s) 757
XceI RCATGY 1 cut(s) 1193
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XhoI CTCGAG 1 cut(s) 375
XmaI CCCGGG 1 cut(s) 1370
XmnI GAANNNNTTC 1 cut(s) 1229
XspI CTAG 2 cut(s) 737, 1574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.