MD00G1149300.v1.1

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010762
Physical Location & Seq
Reverse (-)
32831445 .. 32835074
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1149300.v1.1.491

Sequence Viewer

Length: 1713 bp
ATGTTGGGGTGTGAGTGTTGGTGGTGGAGGAGCAGTGACTACCAATTGTATGATTACTCAGAGCCGCAGCCGCAGCCGCAGCCCTTCTCTCTGCCTTCACCTCTTCCACACTGGCCCCAAGGCGAAGGATTTGCTGCTGGAAGAATACGTCTCGGAGAAATTGAGGTTATCCAAGTCACCAAGTTTGAGAGCATCTGGAGCTGTAGTCTGTTGCGTGGAAAATCAAGGGGTGTCACATTTTATAGACCTGCAGGGATCCCCGATGACTTCTTTTGCCTCGGTTACTACTGCCAGCGGAATGACCAGCCATTGAGAGGGTATGTTCTTGTGGCACGTGAAACGGTTGCCAGAATGGTGGATGATGGTTGTACCCGAGGCTCAGCATTAGAGTTACCGGCTTTAAAAAGGCCGGTTAGCTACTCATTAGTCTGGAACGCGGATTCAAGTAAAAATGGTTGTGGTTATATTTGGTTGCCAAACCCACCTGTTGGTTATAAGGCCATGGGTTTTGTGGTCACTGACAAGCCTGAGGAACCTAGCCCCGAAGAAGTTAGGTGCGTACGAGAAGATCTTACAGAGACTTGTGAAACACATGATCGGATGCTAGCCATGGATTCAAAGTTGGACCAATTTCAGGTGTGGAATACAAGACCCTGCAAACGAGGTATGTTCTACAGTGGTGTTTCCGTTGGTACATTCTTTTGCAGTACATACTTGGATTCCGATGAAGAGCTAGAAGTTGCGTGCTTGAAGAATTTTGATTCGTCTTTACACGCAATGCCAAATCTGAACCAGATTCATTCACTAATTGAGCACTACGGCCCCACGGTGTTCTTCCATCCTAATGAGGTTTATTTGCCATCATCGGTGCAATGGTTTTTCAAAAACGGGGCACTTTTGTACCGGGAAGACACTGGAAATGGCAAACCGATTGATTACAGAGGCTCCAATTTGCCTGCTGGTGGGGAAAATGATAGTGATTTTTGGATAGATTTGCCAAGCGATGATGAAGCGAAAAATCTTCTCAAAGCCGGAAACATAGACAGTGCGGAGCTATACGTTCATGTAAAACCAGCATTGGGAGGAACTTTTACAGATATTGCTATGTGGGTGTTCTGCCCCTTCAATGGACCATCCACCATTAAAATTGGTTTGGTAAATATTGCACTGAACAAGATAGGCGAACATGTCGGTGACTGGGAGCACTTCACTCTCCGAGTGAACAACTTCACGGGAGAACTCTGGCAAGTGTATTTCTCAGAGCACAGCGGTGGCAGATGGGTGGATGCTTTTGATTTGGAGTTCATCGAAGGGAGTAAGCCGATTGTGTACTCATCAAAACACGGTCATAGTAGCTATGCTCATCCCGGGACTTACCTCCAGGGTTCATCCAAGTTTGACATAGGAGTAAGAAATGACGCTGCGCGGAGCAAGTTCTTCATTGATTCGAGCTCCAAGTATCAAATTGTTGCGGCCGAGTACCTTGGCAATGGCGTTATATCCGAACCATCTTGGTTGCAGTATATGCGAGACTGGGGTCCGACCATTGTGTATGATGGACGATCGGAACTAGATAAGTTGATTGATCGCCTTCCCTTCTTTGTTCGATTCTCTGTCGAGAACTTAATCGAGTTGTTTCCTACTCAAATCTATGGCGAGGAAGGGCCAACTGGCCCTAAGGAGAAGGATAATTGGGTAGGAGATGAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

571

Amino Acids

64.57

Weight (kDa)

5.0

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 26 - 569 5.7e-265 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010762)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04350 AT3G04350
fragaria_vesca FvH4_6g01580
malus_domestica MD00G1149300.v1.1 MD04G1232300.v1.1 MD07G1000700.v1.1
prunus_persica Prupe.2G000800_v2.0.a1 Prupe.6G351900_v2.0.a1
pyrus_communis pycom04g20570 pycom12g23000
rosa_chinensis RchiOBHm_Chr3g0449321
rosa_laevigata RLG00000025839
rosa_multiflora Rmu_sc0017357.1_g000003
rosa_roxburghii Rroxscaffold_6G00427330
rosa_rugosa Rorug02G0619700
rosa_samantha Rh3AG017700 Rh3BG017800 Rh3CG016800
rosa_wichuraiana Rw3G001360 Rw3G001390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
Acc36I ACCTGC 1 cut(s) 256
AccB7I CCANNNNNTGG 1 cut(s) 488
AccII CGCG 2 cut(s) 437, 1426
AciI CCGC 9 cut(s) 65, 71, 77, 295, 437, 1049, 1269, 1426, 1472
AclWI GGATC 2 cut(s) 250, 263
AcoI YGGCCR 1 cut(s) 1473
AcsI RAATTY 1 cut(s) 754
AcvI CACGTG 1 cut(s) 335
AfaI GTAC 7 cut(s) 370, 561, 694, 709, 902, 1331, 1481
AfiI CCNNNNNNNGG 6 cut(s) 314, 488, 634, 962, 1079, 1127
AflIII ACRYGT 1 cut(s) 1186
AgsI TTSAA 5 cut(s) 444, 618, 751, 883, 1126
AjnI CCWGG 1 cut(s) 1380
AleI CACNNNNGTG 1 cut(s) 1269
AluBI AGCT 6 cut(s) 201, 417, 733, 1054, 1356, 1452
AluI AGCT 6 cut(s) 201, 417, 733, 1054, 1356, 1452
Alw21I GWGCWC 4 cut(s) 816, 1206, 1266, 1454
Alw26I GTCTC 3 cut(s) 155, 572, 1524
AlwI GGATC 2 cut(s) 250, 263
Ama87I CYCGRG 2 cut(s) 372, 1367
AoxI GGCC 7 cut(s) 113, 407, 498, 820, 1473, 1664, 1672
ApeKI GCWGC 5 cut(s) 67, 73, 79, 134, 1421
ApoI RAATTY 1 cut(s) 754
Asp700I GAANNNNTTC 1 cut(s) 1226
AspLEI GCGC 1 cut(s) 1426
AspS9I GGNCC 7 cut(s) 114, 625, 821, 1130, 1538, 1664, 1673
AsuC2I CCSGG 3 cut(s) 905, 1368, 1369
AsuHPI GGTGA 3 cut(s) 90, 169, 1205
AsuNHI GCTAGC 1 cut(s) 604
AvaI CYCGRG 2 cut(s) 372, 1367
AvaII GGWCC 3 cut(s) 625, 1130, 1538
AxyI CCTNAGG 2 cut(s) 528, 1677
BaeGI GKGCMC 1 cut(s) 895
BaeI ACNNNNGTAYC 2 cut(s) 884, 917
BamHI GGATCC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 1454
BbrPI CACGTG 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 915
Bbv12I GWGCWC 4 cut(s) 816, 1206, 1266, 1454
BbvI GCAGC 5 cut(s) 79, 85, 91, 121, 1408
BccI CCATC 7 cut(s) 356, 846, 868, 1141, 1272, 1516, 1550
BceAI ACGGC 1 cut(s) 835
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 1382
BcnI CCSGG 3 cut(s) 905, 1368, 1369
BcoDI GTCTC 3 cut(s) 155, 572, 1524
BfaI CTAG 4 cut(s) 537, 605, 734, 1571
BfmI CTRYAG 3 cut(s) 202, 249, 673
BfuAI ACCTGC 1 cut(s) 256
BglII AGATCT 1 cut(s) 568
BisI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 135, 1422, 1473
BlpI GCTNAGC 1 cut(s) 379
BlsI GCNGC 9 cut(s) 66, 69, 72, 75, 78, 81, 136, 1423, 1474
Bme1390I CCNGG 4 cut(s) 905, 1368, 1369, 1382
Bme18I GGWCC 3 cut(s) 625, 1130, 1538
BmeT110I CYCGRG 2 cut(s) 372, 1367
BmgT120I GGNCC 7 cut(s) 114, 625, 821, 1130, 1538, 1664, 1673
BmiI GGNNCC 6 cut(s) 116, 257, 534, 823, 946, 1539
BmrFI CCNGG 4 cut(s) 905, 1368, 1369, 1382
BmrI ACTGGG 2 cut(s) 1207, 1543
BmsI GCATC 3 cut(s) 201, 591, 1276
BmtI GCTAGC 1 cut(s) 608
BmuI ACTGGG 2 cut(s) 1207, 1543
BoxI GACNNNNGTC 1 cut(s) 1536
BpiI GAAGAC 1 cut(s) 915
BpmI CTGGAG 2 cut(s) 217, 1364
Bpu1102I GCTNAGC 1 cut(s) 379
BpuMI CCSGG 3 cut(s) 905, 1368, 1369
BsaAI YACGTR 1 cut(s) 335
BsaJI CCNNGG 9 cut(s) 118, 277, 373, 501, 609, 825, 1367, 1381, 1483
BsaXI ACNNNNNCTCC 6 cut(s) 19, 49, 190, 220, 929, 959
Bsc4I CCNNNNNNNGG 6 cut(s) 314, 488, 634, 962, 1079, 1127
Bse118I RCCGGY 2 cut(s) 394, 409
Bse1I ACTGG 5 cut(s) 116, 919, 1202, 1538, 1675
Bse21I CCTNAGG 2 cut(s) 528, 1677
Bse3DI GCAATG 3 cut(s) 783, 878, 1495
BseBI CCWGG 1 cut(s) 1382
BseDI CCNNGG 9 cut(s) 118, 277, 373, 501, 609, 825, 1367, 1381, 1483
BseGI GGATG 7 cut(s) 364, 606, 838, 1133, 1291, 1363, 1388
BseLI CCNNNNNNNGG 6 cut(s) 314, 488, 634, 962, 1079, 1127
BseMI GCAATG 3 cut(s) 783, 878, 1495
BseMII CTCAG 4 cut(s) 72, 393, 519, 1272
BseNI ACTGG 5 cut(s) 116, 919, 1202, 1538, 1675
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 1 cut(s) 895
BseX3I CGGCCG 1 cut(s) 1473
BseXI GCAGC 5 cut(s) 79, 85, 91, 121, 1408
Bsh1236I CGCG 2 cut(s) 437, 1426
Bsh1285I CGRYCG 2 cut(s) 1476, 1565
BshFI GGCC 7 cut(s) 115, 409, 500, 822, 1475, 1666, 1674
BsiEI CGRYCG 2 cut(s) 1476, 1565
BsiHKAI GWGCWC 4 cut(s) 816, 1206, 1266, 1454
BsiHKCI CYCGRG 2 cut(s) 372, 1367
BsiSI CCGG 5 cut(s) 395, 410, 904, 1032, 1368
BsiWI CGTACG 1 cut(s) 559
BslFI GGGAC 1 cut(s) 1384
BslI CCNNNNNNNGG 6 cut(s) 314, 488, 634, 962, 1079, 1127
BsmAI GTCTC 3 cut(s) 155, 572, 1524
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 1384
BsnI GGCC 7 cut(s) 115, 409, 500, 822, 1475, 1666, 1674
BsoBI CYCGRG 2 cut(s) 372, 1367
Bsp1286I GDGCHC 5 cut(s) 816, 895, 1206, 1266, 1454
Bsp143I GATC 5 cut(s) 255, 568, 595, 1562, 1585
Bsp1720I GCTNAGC 1 cut(s) 379
Bsp19I CCATGG 2 cut(s) 501, 609
BspACI CCGC 9 cut(s) 65, 71, 77, 295, 437, 1049, 1269, 1426, 1472
BspANI GGCC 7 cut(s) 115, 409, 500, 822, 1475, 1666, 1674
BspCNI CTCAG 4 cut(s) 71, 392, 520, 1271
BspFNI CGCG 2 cut(s) 437, 1426
BspLI GGNNCC 6 cut(s) 116, 257, 534, 823, 946, 1539
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 256
BspOI GCTAGC 1 cut(s) 608
BspPI GGATC 2 cut(s) 250, 263
BspQI GCTCTTC 1 cut(s) 723
BsrDI GCAATG 3 cut(s) 783, 878, 1495
BsrFI RCCGGY 2 cut(s) 394, 409
BsrI ACTGG 5 cut(s) 116, 919, 1202, 1538, 1675
BssAI RCCGGY 2 cut(s) 394, 409
BssECI CCNNGG 9 cut(s) 118, 277, 373, 501, 609, 825, 1367, 1381, 1483
BssMI GATC 5 cut(s) 255, 568, 595, 1562, 1585
BssT1I CCWWGG 4 cut(s) 118, 501, 609, 1483
Bst2UI CCWGG 1 cut(s) 1382
Bst4CI ACNGT 5 cut(s) 343, 677, 829, 1046, 1346
Bst6I CTCTTC 2 cut(s) 108, 723
BstAPI GCANNNNNTGC 1 cut(s) 1525
BstBAI YACGTR 1 cut(s) 335
BstC8I GCNNGC 4 cut(s) 293, 606, 745, 957
BstDEI CTNAG 5 cut(s) 58, 379, 528, 1258, 1677
BstDSI CCRYGG 3 cut(s) 501, 609, 825
BstF5I GGATG 7 cut(s) 364, 606, 838, 1133, 1291, 1363, 1388
BstFNI CGCG 2 cut(s) 437, 1426
BstHHI GCGC 1 cut(s) 1426
BstKTI GATC 5 cut(s) 258, 571, 598, 1565, 1588
BstMAI GTCTC 3 cut(s) 155, 572, 1524
BstMBI GATC 5 cut(s) 255, 568, 595, 1562, 1585
BstMCI CGRYCG 2 cut(s) 1476, 1565
BstMWI GCNNNNNNNGC 6 cut(s) 70, 73, 76, 79, 198, 1525
BstNI CCWGG 1 cut(s) 1382
BstNSI RCATGY 1 cut(s) 1190
BstPAI GACNNNNGTC 1 cut(s) 1536
BstSCI CCNGG 4 cut(s) 903, 1366, 1367, 1380
BstSFI CTRYAG 3 cut(s) 202, 249, 673
BstSLI GKGCMC 1 cut(s) 895
BstUI CGCG 2 cut(s) 437, 1426
BstV1I GCAGC 5 cut(s) 79, 85, 91, 121, 1408
BstV2I GAAGAC 1 cut(s) 915
BstX2I RGATCY 2 cut(s) 255, 568
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 2 cut(s) 255, 568
BstZI CGGCCG 1 cut(s) 1473
Bsu36I CCTNAGG 2 cut(s) 528, 1677
BsuRI GGCC 7 cut(s) 115, 409, 500, 822, 1475, 1666, 1674
BtgI CCRYGG 3 cut(s) 501, 609, 825
BtgZI GCGATG 1 cut(s) 1017
BtsCI GGATG 7 cut(s) 364, 606, 838, 1133, 1291, 1363, 1388
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 7 cut(s) 40, 109, 516, 682, 912, 1051, 1166
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 4 cut(s) 293, 606, 745, 957
CfoI GCGC 1 cut(s) 1426
Cfr10I RCCGGY 2 cut(s) 394, 409
Cfr13I GGNCC 7 cut(s) 114, 625, 821, 1130, 1538, 1664, 1673
Cfr9I CCCGGG 1 cut(s) 1367
CseI GACGC 1 cut(s) 1427
Csp6I GTAC 7 cut(s) 369, 560, 693, 708, 901, 1330, 1480
CviAII CATG 5 cut(s) 502, 593, 610, 1064, 1187
CviQI GTAC 7 cut(s) 369, 560, 693, 708, 901, 1330, 1480
DdeI CTNAG 5 cut(s) 58, 379, 528, 1258, 1677
DpnI GATC 5 cut(s) 257, 570, 597, 1564, 1587
DpnII GATC 5 cut(s) 255, 568, 595, 1562, 1585
DraI TTTAAA 1 cut(s) 402
EaeI YGGCCR 1 cut(s) 1473
EagI CGGCCG 1 cut(s) 1473
Eam1104I CTCTTC 2 cut(s) 108, 723
EarI CTCTTC 2 cut(s) 108, 723
Ecl136II GAGCTC 1 cut(s) 1452
EclXI CGGCCG 1 cut(s) 1473
Eco130I CCWWGG 4 cut(s) 118, 501, 609, 1483
Eco24I GRGCYC 1 cut(s) 1454
Eco47I GGWCC 3 cut(s) 625, 1130, 1538
Eco52I CGGCCG 1 cut(s) 1473
Eco53kI GAGCTC 1 cut(s) 1452
Eco72I CACGTG 1 cut(s) 335
Eco81I CCTNAGG 2 cut(s) 528, 1677
Eco88I CYCGRG 2 cut(s) 372, 1367
EcoICRI GAGCTC 1 cut(s) 1452
EcoRII CCWGG 1 cut(s) 1380
EcoT14I CCWWGG 4 cut(s) 118, 501, 609, 1483
EcoT38I GRGCYC 1 cut(s) 1454
ErhI CCWWGG 4 cut(s) 118, 501, 609, 1483
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 5 cut(s) 505, 596, 613, 1067, 1190
FaqI GGGAC 1 cut(s) 1384
FatI CATG 5 cut(s) 501, 592, 609, 1063, 1186
Fnu4HI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 135, 1422, 1473
FokI GGATG 7 cut(s) 371, 613, 825, 1120, 1298, 1350, 1375
FriOI GRGCYC 1 cut(s) 1454
Fsp4HI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 135, 1422, 1473
FspBI CTAG 4 cut(s) 537, 605, 734, 1571
GlaI GCGC 1 cut(s) 1425
GluI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 135, 1422, 1473
GsuI CTGGAG 2 cut(s) 217, 1364
HaeIII GGCC 7 cut(s) 115, 409, 500, 822, 1475, 1666, 1674
HapII CCGG 5 cut(s) 395, 410, 904, 1032, 1368
HgaI GACGC 1 cut(s) 1427
HhaI GCGC 1 cut(s) 1426
Hin1II CATG 5 cut(s) 505, 596, 613, 1067, 1190
Hin6I GCGC 1 cut(s) 1424
HinP1I GCGC 1 cut(s) 1424
HinfI GANTC 7 cut(s) 440, 614, 719, 761, 796, 1445, 1608
HpaII CCGG 5 cut(s) 395, 410, 904, 1032, 1368
HphI GGTGA 3 cut(s) 90, 169, 1205
Hpy166II GTNNAC 2 cut(s) 1222, 1330
Hpy188III TCNNGA 3 cut(s) 196, 430, 1618
Hpy8I GTNNAC 2 cut(s) 1222, 1330
HpyAV CCTTC 9 cut(s) 94, 105, 119, 1132, 1304, 1601, 1606, 1655, 1678
HpyCH4III ACNGT 5 cut(s) 343, 677, 829, 1046, 1346
HpyCH4IV ACGT 3 cut(s) 148, 334, 1059
HpyCH4V TGCA 6 cut(s) 251, 657, 705, 871, 1166, 1519
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 73, 76, 79, 198, 1525
HpyF3I CTNAG 5 cut(s) 58, 379, 528, 1258, 1677
HpySE526I ACGT 3 cut(s) 148, 334, 1059
Hsp92II CATG 5 cut(s) 505, 596, 613, 1067, 1190
HspAI GCGC 1 cut(s) 1424
Kzo9I GATC 5 cut(s) 255, 568, 595, 1562, 1585
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 7 cut(s) 30, 198, 950, 1051, 1201, 1428, 1457
Lsp1109I GCAGC 5 cut(s) 79, 85, 91, 121, 1408
LweI GCATC 3 cut(s) 201, 591, 1276
MaeI CTAG 4 cut(s) 537, 605, 734, 1571
MaeII ACGT 3 cut(s) 148, 334, 1059
MaeIII GTNAC 7 cut(s) 35, 175, 232, 281, 390, 514, 1193
MalI GATC 5 cut(s) 257, 570, 597, 1564, 1587
MboI GATC 5 cut(s) 255, 568, 595, 1562, 1585
MfeI CAATTG 1 cut(s) 44
MflI RGATCY 2 cut(s) 255, 568
MhlI GDGCHC 5 cut(s) 816, 895, 1206, 1266, 1454
MluCI AATT 9 cut(s) 44, 159, 629, 754, 807, 949, 1146, 1464, 1690
MmeI TCCRAC 2 cut(s) 603, 1565
MroXI GAANNNNTTC 1 cut(s) 1226
MseI TTAA 3 cut(s) 401, 1143, 1625
MslI CAYNNNNRTG 3 cut(s) 843, 1191, 1269
MspA1I CMGCKG 2 cut(s) 295, 1269
MspI CCGG 5 cut(s) 395, 410, 904, 1032, 1368
MspR9I CCNGG 4 cut(s) 905, 1368, 1369, 1382
MunI CAATTG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 1382
MvnI CGCG 2 cut(s) 437, 1426
MwoI GCNNNNNNNGC 6 cut(s) 70, 73, 76, 79, 198, 1525
NciI CCSGG 3 cut(s) 905, 1368, 1369
NcoI CCATGG 2 cut(s) 501, 609
NdeII GATC 5 cut(s) 255, 568, 595, 1562, 1585
NheI GCTAGC 1 cut(s) 604
NlaIII CATG 5 cut(s) 505, 596, 613, 1067, 1190
NlaIV GGNNCC 6 cut(s) 116, 257, 534, 823, 946, 1539
NmeAIII GCCGAG 1 cut(s) 1501
NmuCI GTSAC 5 cut(s) 35, 175, 232, 514, 1193
NspI RCATGY 1 cut(s) 1190
OliI CACNNNNGTG 1 cut(s) 1269
PciI ACATGT 1 cut(s) 1186
PciSI GCTCTTC 1 cut(s) 723
PdmI GAANNNNTTC 1 cut(s) 1226
PfeI GAWTC 7 cut(s) 440, 614, 719, 761, 796, 1445, 1608
Pfl23II CGTACG 1 cut(s) 559
PflMI CCANNNNNTGG 1 cut(s) 488
PkrI GCNGC 9 cut(s) 66, 69, 72, 75, 78, 81, 136, 1423, 1474
Ple19I CGATCG 1 cut(s) 1565
PmaCI CACGTG 1 cut(s) 335
PmlI CACGTG 1 cut(s) 335
Ppu21I YACGTR 1 cut(s) 335
PscI ACATGT 1 cut(s) 1186
PshAI GACNNNNGTC 1 cut(s) 1536
PsiI TTATAA 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 1454
Psp6I CCWGG 1 cut(s) 1380
PspCI CACGTG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 1380
PspLI CGTACG 1 cut(s) 559
PspN4I GGNNCC 6 cut(s) 116, 257, 534, 823, 946, 1539
PspPI GGNCC 7 cut(s) 114, 625, 821, 1130, 1538, 1664, 1673
PstI CTGCAG 1 cut(s) 253
PsuI RGATCY 2 cut(s) 255, 568
PvuI CGATCG 1 cut(s) 1565
RsaI GTAC 7 cut(s) 370, 561, 694, 709, 902, 1331, 1481
RsaNI GTAC 7 cut(s) 369, 560, 693, 708, 901, 1330, 1480
RseI CAYNNNNRTG 3 cut(s) 843, 1191, 1269
SacI GAGCTC 1 cut(s) 1454
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 3 cut(s) 401, 1143, 1625
SatI GCNGC 9 cut(s) 65, 68, 71, 74, 77, 80, 135, 1422, 1473
Sau3AI GATC 5 cut(s) 255, 568, 595, 1562, 1585
Sau96I GGNCC 7 cut(s) 114, 625, 821, 1130, 1538, 1664, 1673
SbfI CCTGCAGG 1 cut(s) 253
ScrFI CCNGG 4 cut(s) 905, 1368, 1369, 1382
SdaI CCTGCAGG 1 cut(s) 253
SduI GDGCHC 5 cut(s) 816, 895, 1206, 1266, 1454
SfaNI GCATC 3 cut(s) 201, 591, 1276
SfcI CTRYAG 3 cut(s) 202, 249, 673
SinI GGWCC 3 cut(s) 625, 1130, 1538
SmaI CCCGGG 1 cut(s) 1369
SmiMI CAYNNNNRTG 3 cut(s) 843, 1191, 1269
Sse8387I CCTGCAGG 1 cut(s) 253
Sse9I AATT 9 cut(s) 44, 159, 629, 754, 807, 949, 1146, 1464, 1690
SsiI CCGC 9 cut(s) 65, 71, 77, 295, 437, 1049, 1269, 1426, 1472
SspI AATATT 1 cut(s) 1162
SspMI CTAG 4 cut(s) 537, 605, 734, 1571
SstI GAGCTC 1 cut(s) 1454
StyD4I CCNGG 4 cut(s) 903, 1366, 1367, 1380
StyI CCWWGG 4 cut(s) 118, 501, 609, 1483
TaaI ACNGT 5 cut(s) 343, 677, 829, 1046, 1346
TaiI ACGT 3 cut(s) 151, 337, 1062
TaqI TCGA 5 cut(s) 1308, 1448, 1606, 1617, 1629
TasI AATT 9 cut(s) 44, 159, 629, 754, 807, 949, 1146, 1464, 1690
TatI WGTACW 2 cut(s) 707, 1329
TauI GCSGC 4 cut(s) 67, 73, 79, 1475
TfiI GAWTC 7 cut(s) 440, 614, 719, 761, 796, 1445, 1608
Tru1I TTAA 3 cut(s) 401, 1143, 1625
Tru9I TTAA 3 cut(s) 401, 1143, 1625
TscAI CASTG 7 cut(s) 40, 116, 523, 682, 919, 1051, 1173
TseFI GTSAC 5 cut(s) 35, 175, 232, 514, 1193
TseI GCWGC 5 cut(s) 67, 73, 79, 134, 1421
Tsp45I GTSAC 5 cut(s) 35, 175, 232, 514, 1193
TspDTI ATGAA 7 cut(s) 741, 788, 1023, 1052, 1294, 1377, 1429
TspGWI ACGGA 1 cut(s) 676
TspMI CCCGGG 1 cut(s) 1367
TspRI CASTG 7 cut(s) 40, 116, 523, 682, 919, 1051, 1173
Van91I CCANNNNNTGG 1 cut(s) 488
VpaK11BI GGWCC 3 cut(s) 625, 1130, 1538
XapI RAATTY 1 cut(s) 754
XceI RCATGY 1 cut(s) 1190
XcmI CCANNNNNNNNNTGG 1 cut(s) 508
XmaI CCCGGG 1 cut(s) 1367
XmnI GAANNNNTTC 1 cut(s) 1226
XspI CTAG 4 cut(s) 537, 605, 734, 1571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.