pycom04g20570

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010762
Physical Location & Seq
Reverse (-)
22036575 .. 22039440
2866 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20570.2

Sequence Viewer

Length: 1695 bp
ATGTTGGGGTGTGAGTGTTGGTGGTGGAGCAGCAGTGAGTACCAATTGTATGATTACTCAGAGCCACAGCCCTTCTCTCTGCCTTCACCTCTTCCACACTGGCCTCAAGGCGAAGGATTTGCTACTGGAAGAATACGCCTCGGAGAAATTGAAGTTAGCCAAATAACCAAGTTTGAGAGCATCTGGAGCTGCAATCTGTTGCGTGGAAAATCAAAGGGTGTCACATTTTATAGACCTGCAGGGATCCCTGATGGCTTCTTTTGCCTCGGTTACTACTGCCAGCCCAATGACCAGCCATTGCAAGGGTATGTTCTTGTGGCTCGTGAAACAGTTGCCAGAAGGGCGGAAGATGGTTGTACCCCGGACTCAGCATTAGAGTTACCAGCTTTAGAAAAACCGGTTAACTACTCGTTAGTCTGCAATGCAGATTTAAGTGAAAATGGTTGTGGTTATATTTGGTTGCCAAACCCACCTGTTGGTTATAAGGCCATGGGTTTTGTGGTCACTGACATGCCTGAGGAACCCAGCCCCGAAGAAGTTAGATGCGTACGAGAAGATCTTACAGAGACTTGTGAAACATGTGATCGGATACTTGCAATGGATTCAAAATTTGAGGTGTGGAATACACGACCCTGCAAACGGGGTATGTTTTGCACTGGTGTTCCGGTGGGTACATTCTTTTGCAGTACCTACTTGGATTCTAATGAAGAGCTACAAGTTTCTTGCTTGAAGAATTTTGATTCGTCCTTGCACGCAATGCCAAATCTGAACCAGATTCATTCGCTAATTGAGCACTACGGTCCCACAGTGTTCTTCCATCCTAATGAGGTTTATATGCCATCTTCGGTACAATGGTTTTTCAAAAATGGGGCACTTTTGTACCAGGAAGACACTGGGAATGGTGAACCGATTGATCACAGAGGCTCCAATTTGCCTGGTGGAGGGGAAAATGACAGTGATTTTTGGATAGATTTGCCAAATGACGATGAAGTTAAAAATCTTATCAAGGCTGGAAACATAGACAGCGCGGAGCTATACGTTCATGTAAAACCAGCACTGGGAGGAACTTTTACAGATATTGCCATGTGGGTGTTCTGCCCCTTCAACGGACCATCCGCCATTAAAATTGGTTTGGTAAGTTTTGCATTGAACAAGATAGGCGAACATGTCGGTGACTGGGAGCACTTCACTCTCCGAGTGAACAACTTCACCGGAGAACTCTGGCAAGTGTACTTCTCAGAGCACAGCGGTGGCAGATGGGTGGATGCTTTTGACTTGGAGTTCATTGAAGGCAATAAGCCGATTGTGTATTCTTCAAAACACGGTCATAGCAGCTACGCTCATCCCGGGACTTACCTCCAGGGTTCATCAAAGTTTGACATAGGGGTAAGGAATGACGCTGCTCGGAGCAAGTTTTTCATTGATTCGAGCACCAAGTATCAAATTGTCGCGGCCGAGTACCTTGGCAAAGGCGTTATATCCGAACCATGTTGGTTGCAGTATATGCGAGACTGGGGTCCGACCATTGTGTATGATGGACGAGAGGAACTAGATAAGTTGATTGATCGTCTTCCGTTCTTTGTTCGATTCTCAGTCGAGAACTTAATCGAGTTGTTTCCTACGCAACTCTATGGGGAGGAAGGACCAACTGGCCCTAAGGAGAAGGACAATTGGTTAGGAGATGAAAGATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

565

Amino Acids

63.67

Weight (kDa)

4.75

Isoelectric Point (pI)

48.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010762)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04350 AT3G04350
fragaria_vesca FvH4_6g01580
malus_domestica MD00G1149300.v1.1 MD04G1232300.v1.1 MD07G1000700.v1.1
prunus_persica Prupe.2G000800_v2.0.a1 Prupe.6G351900_v2.0.a1
pyrus_communis pycom04g20570 pycom12g23000
rosa_chinensis RchiOBHm_Chr3g0449321
rosa_laevigata RLG00000025839
rosa_multiflora Rmu_sc0017357.1_g000003
rosa_roxburghii Rroxscaffold_6G00427330
rosa_rugosa Rorug02G0619700
rosa_samantha Rh3AG017700 Rh3BG017800 Rh3CG016800
rosa_wichuraiana Rw3G001360 Rw3G001390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
Acc36I ACCTGC 1 cut(s) 244
AccB7I CCANNNNNTGG 1 cut(s) 476
AccII CGCG 2 cut(s) 1028, 1451
AciI CCGC 5 cut(s) 344, 1028, 1116, 1248, 1451
AclWI GGATC 2 cut(s) 238, 251
AcoI YGGCCR 1 cut(s) 1452
AcsI RAATTY 2 cut(s) 608, 733
AfaI GTAC 9 cut(s) 41, 358, 549, 673, 688, 849, 881, 1232, 1460
AfiI CCNNNNNNNGG 6 cut(s) 302, 476, 639, 941, 1058, 1106
AflIII ACRYGT 2 cut(s) 578, 1165
AgeI ACCGGT 1 cut(s) 397
AgsI TTSAA 8 cut(s) 152, 606, 730, 862, 1105, 1150, 1289, 1317
AjnI CCWGG 3 cut(s) 882, 934, 1359
AleI CACNNNNGTG 1 cut(s) 1248
AluBI AGCT 5 cut(s) 189, 386, 712, 1033, 1335
AluI AGCT 5 cut(s) 189, 386, 712, 1033, 1335
Alw21I GWGCWC 4 cut(s) 795, 1185, 1245, 1433
Alw26I GTCTC 2 cut(s) 560, 1503
AlwI GGATC 2 cut(s) 238, 251
Ama87I CYCGRG 1 cut(s) 1346
AoxI GGCC 4 cut(s) 101, 486, 1452, 1651
ApeKI GCWGC 4 cut(s) 30, 189, 1332, 1400
ApoI RAATTY 2 cut(s) 608, 733
AsiGI ACCGGT 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 1205
AspLEI GCGC 1 cut(s) 1028
AspS9I GGNCC 5 cut(s) 800, 1109, 1517, 1643, 1652
AsuC2I CCSGG 3 cut(s) 362, 1347, 1348
AsuHPI GGTGA 4 cut(s) 78, 914, 1184, 1201
AvaI CYCGRG 1 cut(s) 1346
AvaII GGWCC 4 cut(s) 800, 1109, 1517, 1643
AxyI CCTNAGG 2 cut(s) 516, 1656
BaeGI GKGCMC 1 cut(s) 874
BaeI ACNNNNGTAYC 4 cut(s) 839, 863, 872, 896
BamHI GGATCC 1 cut(s) 243
BauI CACGAG 1 cut(s) 321
BbsI GAAGAC 2 cut(s) 894, 1562
Bbv12I GWGCWC 4 cut(s) 795, 1185, 1245, 1433
BbvI GCAGC 4 cut(s) 42, 176, 1344, 1387
BccI CCATC 7 cut(s) 245, 344, 825, 847, 1120, 1251, 1529
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 3 cut(s) 884, 936, 1361
BciVI GTATCC 1 cut(s) 582
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 3 cut(s) 362, 1347, 1348
BcoDI GTCTC 2 cut(s) 560, 1503
BfaI CTAG 2 cut(s) 1550, 1693
BfmI CTRYAG 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 244
BfuI GTATCC 1 cut(s) 582
BglI GCCNNNNNGGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 556
BisI GCNGC 5 cut(s) 31, 190, 1333, 1401, 1452
BlsI GCNGC 5 cut(s) 32, 191, 1334, 1402, 1453
Bme1390I CCNGG 6 cut(s) 362, 884, 936, 1347, 1348, 1361
Bme18I GGWCC 4 cut(s) 800, 1109, 1517, 1643
BmeT110I CYCGRG 1 cut(s) 1346
BmgT120I GGNCC 5 cut(s) 800, 1109, 1517, 1643, 1652
BmiI GGNNCC 5 cut(s) 245, 522, 802, 925, 1518
BmrFI CCNGG 6 cut(s) 362, 884, 936, 1347, 1348, 1361
BmrI ACTGGG 4 cut(s) 903, 1067, 1186, 1522
BmsI GCATC 4 cut(s) 189, 533, 1255, 1679
BmuI ACTGGG 4 cut(s) 903, 1067, 1186, 1522
BoxI GACNNNNGTC 1 cut(s) 1515
BpiI GAAGAC 2 cut(s) 894, 1562
BpmI CTGGAG 2 cut(s) 205, 1343
BpuEI CTTGAG 1 cut(s) 90
BpuMI CCSGG 3 cut(s) 362, 1347, 1348
BsaJI CCNNGG 7 cut(s) 139, 265, 360, 489, 1346, 1360, 1462
BsaWI WCCGGW 3 cut(s) 397, 664, 1211
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 908, 938
Bsc4I CCNNNNNNNGG 6 cut(s) 302, 476, 639, 941, 1058, 1106
Bse118I RCCGGY 1 cut(s) 397
Bse1I ACTGG 8 cut(s) 104, 130, 661, 898, 1062, 1181, 1517, 1654
Bse21I CCTNAGG 2 cut(s) 516, 1656
Bse3DI GCAATG 4 cut(s) 296, 427, 603, 762
BseBI CCWGG 3 cut(s) 884, 936, 1361
BseDI CCNNGG 7 cut(s) 139, 265, 360, 489, 1346, 1360, 1462
BseGI GGATG 4 cut(s) 817, 1112, 1270, 1342
BseLI CCNNNNNNNGG 6 cut(s) 302, 476, 639, 941, 1058, 1106
BseMI GCAATG 4 cut(s) 296, 427, 603, 762
BseMII CTCAG 5 cut(s) 72, 381, 507, 1251, 1605
BseNI ACTGG 8 cut(s) 104, 130, 661, 898, 1062, 1181, 1517, 1654
BseSI GKGCMC 1 cut(s) 874
BseX3I CGGCCG 1 cut(s) 1452
BseXI GCAGC 4 cut(s) 42, 176, 1344, 1387
BseYI CCCAGC 1 cut(s) 524
Bsh1236I CGCG 2 cut(s) 1028, 1451
Bsh1285I CGRYCG 1 cut(s) 1455
BshFI GGCC 4 cut(s) 103, 488, 1454, 1653
BshTI ACCGGT 1 cut(s) 397
BsiEI CGRYCG 1 cut(s) 1455
BsiHKAI GWGCWC 4 cut(s) 795, 1185, 1245, 1433
BsiHKCI CYCGRG 1 cut(s) 1346
BsiSI CCGG 5 cut(s) 362, 398, 665, 1212, 1347
BsiWI CGTACG 1 cut(s) 547
BslFI GGGAC 2 cut(s) 786, 1363
BslI CCNNNNNNNGG 6 cut(s) 302, 476, 639, 941, 1058, 1106
BsmAI GTCTC 2 cut(s) 560, 1503
BsmFI GGGAC 2 cut(s) 786, 1363
BsnI GGCC 4 cut(s) 103, 488, 1454, 1653
BsoBI CYCGRG 1 cut(s) 1346
Bsp1286I GDGCHC 5 cut(s) 795, 874, 1185, 1245, 1433
Bsp143I GATC 5 cut(s) 243, 556, 583, 913, 1564
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 5 cut(s) 344, 1028, 1116, 1248, 1451
BspANI GGCC 4 cut(s) 103, 488, 1454, 1653
BspCNI CTCAG 5 cut(s) 71, 380, 508, 1250, 1604
BspFNI CGCG 2 cut(s) 1028, 1451
BspLI GGNNCC 5 cut(s) 245, 522, 802, 925, 1518
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 2 cut(s) 238, 251
BspQI GCTCTTC 1 cut(s) 702
BsrDI GCAATG 4 cut(s) 296, 427, 603, 762
BsrFI RCCGGY 1 cut(s) 397
BsrI ACTGG 8 cut(s) 104, 130, 661, 898, 1062, 1181, 1517, 1654
BssAI RCCGGY 1 cut(s) 397
BssECI CCNNGG 7 cut(s) 139, 265, 360, 489, 1346, 1360, 1462
BssMI GATC 5 cut(s) 243, 556, 583, 913, 1564
BssSI CACGAG 1 cut(s) 321
BssT1I CCWWGG 2 cut(s) 489, 1462
Bst2BI CACGAG 1 cut(s) 321
Bst2UI CCWGG 3 cut(s) 884, 936, 1361
Bst4CI ACNGT 5 cut(s) 331, 800, 808, 956, 1325
Bst6I CTCTTC 2 cut(s) 96, 702
BstAPI GCANNNNNTGC 2 cut(s) 757, 1504
BstC8I GCNNGC 2 cut(s) 281, 753
BstDEI CTNAG 6 cut(s) 58, 367, 516, 1237, 1591, 1656
BstDSI CCRYGG 1 cut(s) 489
BstENI CCTNNNNNAGG 1 cut(s) 939
BstF5I GGATG 4 cut(s) 817, 1112, 1270, 1342
BstFNI CGCG 2 cut(s) 1028, 1451
BstHHI GCGC 1 cut(s) 1028
BstKTI GATC 5 cut(s) 246, 559, 586, 916, 1567
BstMAI GTCTC 2 cut(s) 560, 1503
BstMBI GATC 5 cut(s) 243, 556, 583, 913, 1564
BstMCI CGRYCG 1 cut(s) 1455
BstMWI GCNNNNNNNGC 6 cut(s) 186, 261, 341, 757, 790, 1504
BstNI CCWGG 3 cut(s) 884, 936, 1361
BstNSI RCATGY 3 cut(s) 514, 582, 1169
BstPAI GACNNNNGTC 1 cut(s) 1515
BstSCI CCNGG 6 cut(s) 360, 882, 934, 1345, 1346, 1359
BstSFI CTRYAG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 874
BstUI CGCG 2 cut(s) 1028, 1451
BstV1I GCAGC 4 cut(s) 42, 176, 1344, 1387
BstV2I GAAGAC 2 cut(s) 894, 1562
BstX2I RGATCY 2 cut(s) 243, 556
BstYI RGATCY 2 cut(s) 243, 556
BstZI CGGCCG 1 cut(s) 1452
Bsu36I CCTNAGG 2 cut(s) 516, 1656
BsuI GTATCC 1 cut(s) 582
BsuRI GGCC 4 cut(s) 103, 488, 1454, 1653
BtgI CCRYGG 1 cut(s) 489
BtsCI GGATG 4 cut(s) 817, 1112, 1270, 1342
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 8 cut(s) 40, 97, 504, 654, 813, 891, 961, 1055
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 2 cut(s) 281, 753
CfoI GCGC 1 cut(s) 1028
Cfr10I RCCGGY 1 cut(s) 397
Cfr13I GGNCC 5 cut(s) 800, 1109, 1517, 1643, 1652
Cfr9I CCCGGG 1 cut(s) 1346
CseI GACGC 1 cut(s) 1406
Csp6I GTAC 9 cut(s) 40, 357, 548, 672, 687, 848, 880, 1231, 1459
CspAI ACCGGT 1 cut(s) 397
CviAII CATG 7 cut(s) 490, 511, 579, 1043, 1084, 1166, 1488
CviQI GTAC 9 cut(s) 40, 357, 548, 672, 687, 848, 880, 1231, 1459
DdeI CTNAG 6 cut(s) 58, 367, 516, 1237, 1591, 1656
DpnI GATC 5 cut(s) 245, 558, 585, 915, 1566
DpnII GATC 5 cut(s) 243, 556, 583, 913, 1564
EaeI YGGCCR 1 cut(s) 1452
EagI CGGCCG 1 cut(s) 1452
Eam1104I CTCTTC 2 cut(s) 96, 702
EarI CTCTTC 2 cut(s) 96, 702
EciI GGCGGA 2 cut(s) 359, 1105
EclXI CGGCCG 1 cut(s) 1452
Eco130I CCWWGG 2 cut(s) 489, 1462
Eco47I GGWCC 4 cut(s) 800, 1109, 1517, 1643
Eco52I CGGCCG 1 cut(s) 1452
Eco81I CCTNAGG 2 cut(s) 516, 1656
Eco88I CYCGRG 1 cut(s) 1346
EcoNI CCTNNNNNAGG 1 cut(s) 939
EcoRII CCWGG 3 cut(s) 882, 934, 1359
EcoT14I CCWWGG 2 cut(s) 489, 1462
ErhI CCWWGG 2 cut(s) 489, 1462
FaeI CATG 7 cut(s) 493, 514, 582, 1046, 1087, 1169, 1491
FaqI GGGAC 2 cut(s) 786, 1363
FatI CATG 7 cut(s) 489, 510, 578, 1042, 1083, 1165, 1487
FbaI TGATCA 1 cut(s) 913
Fnu4HI GCNGC 5 cut(s) 31, 190, 1333, 1401, 1452
FokI GGATG 4 cut(s) 804, 1099, 1277, 1329
Fsp4HI GCNGC 5 cut(s) 31, 190, 1333, 1401, 1452
FspBI CTAG 2 cut(s) 1550, 1693
GlaI GCGC 1 cut(s) 1027
GluI GCNGC 5 cut(s) 31, 190, 1333, 1401, 1452
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 2 cut(s) 205, 1343
HaeIII GGCC 4 cut(s) 103, 488, 1454, 1653
HapII CCGG 5 cut(s) 362, 398, 665, 1212, 1347
HgaI GACGC 1 cut(s) 1406
HhaI GCGC 1 cut(s) 1028
Hin1II CATG 7 cut(s) 493, 514, 582, 1046, 1087, 1169, 1491
Hin6I GCGC 1 cut(s) 1026
HinP1I GCGC 1 cut(s) 1026
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 7 cut(s) 365, 602, 698, 740, 775, 1424, 1587
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 5 cut(s) 362, 398, 665, 1212, 1347
HphI GGTGA 4 cut(s) 78, 914, 1184, 1201
Hpy166II GTNNAC 4 cut(s) 403, 905, 1201, 1231
Hpy188I TCNGA 9 cut(s) 61, 143, 588, 768, 1196, 1240, 1407, 1483, 1521
Hpy188III TCNNGA 3 cut(s) 184, 323, 1597
Hpy8I GTNNAC 4 cut(s) 403, 905, 1201, 1231
HpyAV CCTTC 8 cut(s) 82, 93, 107, 333, 1111, 1283, 1634, 1657
HpyCH4III ACNGT 5 cut(s) 331, 800, 808, 956, 1325
HpyCH4IV ACGT 1 cut(s) 1038
HpyF10VI GCNNNNNNNGC 6 cut(s) 186, 261, 341, 757, 790, 1504
HpyF3I CTNAG 6 cut(s) 58, 367, 516, 1237, 1591, 1656
HpySE526I ACGT 1 cut(s) 1038
Hsp92II CATG 7 cut(s) 493, 514, 582, 1046, 1087, 1169, 1491
HspAI GCGC 1 cut(s) 1026
Ksp22I TGATCA 1 cut(s) 913
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 5 cut(s) 243, 556, 583, 913, 1564
LguI GCTCTTC 1 cut(s) 702
LmnI GCTCC 6 cut(s) 27, 186, 929, 1030, 1180, 1407
Lsp1109I GCAGC 4 cut(s) 42, 176, 1344, 1387
LweI GCATC 4 cut(s) 189, 533, 1255, 1679
MaeI CTAG 2 cut(s) 1550, 1693
MaeII ACGT 1 cut(s) 1038
MaeIII GTNAC 5 cut(s) 220, 269, 378, 502, 1172
MalI GATC 5 cut(s) 245, 558, 585, 915, 1566
MboI GATC 5 cut(s) 243, 556, 583, 913, 1564
MfeI CAATTG 2 cut(s) 44, 1669
MflI RGATCY 2 cut(s) 243, 556
MhlI GDGCHC 5 cut(s) 795, 874, 1185, 1245, 1433
MluCI AATT 9 cut(s) 44, 147, 608, 733, 786, 928, 1125, 1443, 1669
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 1544
MroXI GAANNNNTTC 1 cut(s) 1205
MseI TTAA 5 cut(s) 402, 431, 993, 1122, 1604
MslI CAYNNNNRTG 5 cut(s) 509, 822, 1088, 1170, 1248
MspA1I CMGCKG 1 cut(s) 1248
MspI CCGG 5 cut(s) 362, 398, 665, 1212, 1347
MspR9I CCNGG 6 cut(s) 362, 884, 936, 1347, 1348, 1361
MunI CAATTG 2 cut(s) 44, 1669
MvaI CCWGG 3 cut(s) 884, 936, 1361
MvnI CGCG 2 cut(s) 1028, 1451
MwoI GCNNNNNNNGC 6 cut(s) 186, 261, 341, 757, 790, 1504
NciI CCSGG 3 cut(s) 362, 1347, 1348
NcoI CCATGG 1 cut(s) 489
NdeII GATC 5 cut(s) 243, 556, 583, 913, 1564
NlaIII CATG 7 cut(s) 493, 514, 582, 1046, 1087, 1169, 1491
NlaIV GGNNCC 5 cut(s) 245, 522, 802, 925, 1518
NmeAIII GCCGAG 1 cut(s) 1480
NmuCI GTSAC 3 cut(s) 220, 502, 1172
NspI RCATGY 3 cut(s) 514, 582, 1169
OliI CACNNNNGTG 1 cut(s) 1248
PciI ACATGT 2 cut(s) 578, 1165
PciSI GCTCTTC 1 cut(s) 702
PdmI GAANNNNTTC 1 cut(s) 1205
PfeI GAWTC 6 cut(s) 602, 698, 740, 775, 1424, 1587
Pfl23II CGTACG 1 cut(s) 547
PflMI CCANNNNNTGG 1 cut(s) 476
PinAI ACCGGT 1 cut(s) 397
PkrI GCNGC 5 cut(s) 32, 191, 1334, 1402, 1453
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
PscI ACATGT 2 cut(s) 578, 1165
PshAI GACNNNNGTC 1 cut(s) 1515
PsiI TTATAA 1 cut(s) 483
Psp6I CCWGG 3 cut(s) 882, 934, 1359
PspFI CCCAGC 1 cut(s) 524
PspGI CCWGG 3 cut(s) 882, 934, 1359
PspLI CGTACG 1 cut(s) 547
PspN4I GGNNCC 5 cut(s) 245, 522, 802, 925, 1518
PspPI GGNCC 5 cut(s) 800, 1109, 1517, 1643, 1652
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 243, 556
RsaI GTAC 9 cut(s) 41, 358, 549, 673, 688, 849, 881, 1232, 1460
RsaNI GTAC 9 cut(s) 40, 357, 548, 672, 687, 848, 880, 1231, 1459
RseI CAYNNNNRTG 5 cut(s) 509, 822, 1088, 1170, 1248
SapI GCTCTTC 1 cut(s) 702
SaqAI TTAA 5 cut(s) 402, 431, 993, 1122, 1604
SatI GCNGC 5 cut(s) 31, 190, 1333, 1401, 1452
Sau3AI GATC 5 cut(s) 243, 556, 583, 913, 1564
Sau96I GGNCC 5 cut(s) 800, 1109, 1517, 1643, 1652
SbfI CCTGCAGG 1 cut(s) 241
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 6 cut(s) 362, 884, 936, 1347, 1348, 1361
SdaI CCTGCAGG 1 cut(s) 241
SduI GDGCHC 5 cut(s) 795, 874, 1185, 1245, 1433
SfaNI GCATC 4 cut(s) 189, 533, 1255, 1679
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 4 cut(s) 800, 1109, 1517, 1643
SmaI CCCGGG 1 cut(s) 1348
SmiMI CAYNNNNRTG 5 cut(s) 509, 822, 1088, 1170, 1248
SmlI CTYRAG 1 cut(s) 105
SmoI CTYRAG 1 cut(s) 105
Sse8387I CCTGCAGG 1 cut(s) 241
Sse9I AATT 9 cut(s) 44, 147, 608, 733, 786, 928, 1125, 1443, 1669
SsiI CCGC 5 cut(s) 344, 1028, 1116, 1248, 1451
SspMI CTAG 2 cut(s) 1550, 1693
StyD4I CCNGG 6 cut(s) 360, 882, 934, 1345, 1346, 1359
StyI CCWWGG 2 cut(s) 489, 1462
TaaI ACNGT 5 cut(s) 331, 800, 808, 956, 1325
TaiI ACGT 1 cut(s) 1041
TaqI TCGA 4 cut(s) 1427, 1585, 1596, 1608
TasI AATT 9 cut(s) 44, 147, 608, 733, 786, 928, 1125, 1443, 1669
TatI WGTACW 1 cut(s) 1230
TauI GCSGC 1 cut(s) 1454
TfiI GAWTC 6 cut(s) 602, 698, 740, 775, 1424, 1587
Tru1I TTAA 5 cut(s) 402, 431, 993, 1122, 1604
Tru9I TTAA 5 cut(s) 402, 431, 993, 1122, 1604
TscAI CASTG 8 cut(s) 40, 104, 511, 661, 813, 898, 961, 1062
TseFI GTSAC 3 cut(s) 220, 502, 1172
TseI GCWGC 4 cut(s) 30, 189, 1332, 1400
Tsp45I GTSAC 3 cut(s) 220, 502, 1172
TspDTI ATGAA 7 cut(s) 720, 767, 1002, 1031, 1273, 1356, 1408
TspGWI ACGGA 2 cut(s) 1122, 1563
TspMI CCCGGG 1 cut(s) 1346
TspRI CASTG 8 cut(s) 40, 104, 511, 661, 813, 898, 961, 1062
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 4 cut(s) 800, 1109, 1517, 1643
XagI CCTNNNNNAGG 1 cut(s) 939
XapI RAATTY 2 cut(s) 608, 733
XceI RCATGY 3 cut(s) 514, 582, 1169
XcmI CCANNNNNNNNNTGG 2 cut(s) 496, 890
XmaI CCCGGG 1 cut(s) 1346
XmnI GAANNNNTTC 1 cut(s) 1205
XspI CTAG 2 cut(s) 1550, 1693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.